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副猪嗜血杆菌毒力因子研究进展 总被引：1，自引：0，他引：1
副猪嗜血杆菌(Haemophilus parasuis)是引起猪革拉泽氏病(Glsser’s disease)的病原,能在特定的条件下侵入机体而引起严重的全身性疾病,以纤维素性多发性浆膜炎、关节炎和脑膜炎为主要特征。副猪嗜血杆菌的毒力因子仍不十分清楚。H.parasuis的全基因组测序工作的完成和遗传操作系统的建立极大促进了毒力因子、致病机制和基因功能的研究。近年证实了H.parasuis的外膜蛋白(Omp)和脂多糖(LPS)与毒力有一定的联系,特别是OmpP2、OmpP5和LPS的庚糖基转移。为了进一步鉴定毒力因子,利用在体外模拟体内感染环境和比较基因组学等方法鉴定了H.parasuis潜在的毒力因子。论文对H.pa-rasuis的毒力因子进行综述,以供参考。 相似文献
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副猪嗜血杆菌抗原性研究进展 总被引：2，自引：0，他引：2
副猪嗜血杆菌是猪格拉瑟氏病的病原,主要引起SPF猪和断奶前后仔猪的纤维素性多发性浆膜炎、多发性关节炎和脑膜炎等。其抗原主要有荚膜、脂多糖和外膜蛋白等,不是所有的菌株都存在荚膜抗原,外膜蛋白抗原是刺激机体产生体液免疫的主要抗原,菌株抗原性的高低与毒力的强弱呈正相关,副猪嗜血杆菌的毒力因子至今仍不清楚,推测其荚膜、外膜蛋白、菌体细胞膜表面的自动传输三聚体蛋白等与副猪嗜血杆菌的毒力有关。至今已经定型的副猪嗜血杆菌有15个血清型,还有约30%为未定型的菌株,其中血清5型副猪嗜血杆菌为强毒力代表菌株,常作为研究副猪嗜血杆菌抗原性的代表菌株。副猪嗜血杆菌抗原性的深入研究必将为新型、高效疫苗的研究奠定坚实的基础。 相似文献
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《畜牧与兽医》2016,(8):1-7
为了掌握浙江省近几年副猪嗜血杆菌(Haemophilus parasuis,HPs)的血清型流行情况,通过PCR扩增的方法来鉴定浙江省2010—2015年从113个猪场中分离鉴定的副猪嗜血杆菌血清型,同时检测6个可能与副猪嗜血杆菌毒力相关的基因(B14、D45、nha C、fhu A、hhd A、hhd B),并分析其与血清型的相关性。结果显示:浙江省副猪嗜血杆菌近几年的优势血清型为5/12型、4型、13型和14型,未检测到3型、9型、11型和15型;2010—2015年副猪嗜血杆菌的主要血清型流行趋势并未发生明显改变,部分猪场存在2种血清型菌株共感染现象;对潜在毒力基因的检测结果表明,D45基因在毒力菌株中出现的比例(82.4%~87.5%)高于其在无毒力菌株中的检测比例(50%);fhu A基因在强毒力菌株中的检测比例(55%)却低于其在中等毒力和无毒力菌株中的检测比例(70.6%~90%);其他4个毒力基因(B14、nha C、hhd A、hhd B)在所有菌株中均有较高的比例。提示:副猪嗜血杆菌潜在的毒力基因与血清型之间并没有明显的相关性。 相似文献
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副猪嗜血杆菌15个血清型标准分离株对小鼠毒力的研究 总被引：1，自引：0，他引：1
为了确定Balb/c小鼠是否适合作为副猪嗜血杆菌(Haemophilus parasuis,HPS)的试验动物模型,试验用1～15血清型HPS标准菌株感染Balb/c小鼠,每只小鼠接种量为1.0×109 cfu。结果表明:用血清型1,2,5,10,12,13,14接种的5只小鼠均死亡4只,用血清型4,15接种的5只小鼠分别死亡2,3只,用血清型3,6,7,8,9,11接种的5只小鼠均没有死亡;各血清型的毒力与猪体试验的结果基本相符。说明Balb/c小鼠适合作为副猪嗜血杆菌的试验动物模型。 相似文献
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副猪嗜血杆菌主要毒力因子和致病机理的研究进展 总被引：4，自引：0，他引：4
副猪嗜血杆菌病又称革拉斯氏病(Glasser’s disease),引起猪的多发性浆膜炎、关节炎和脑膜脑炎,是近年来严重危害养猪业的细菌性传染病,呈世界性分布,并已证实在中国绝大多数猪场存在和流行。副猪嗜血杆菌的血清型众多,不同血清型的副猪嗜血杆菌毒力差异很大,对其毒力因子了解很少,对其主要的致病机理更是知之甚少,现已发现该病原可能的毒力相关因子有荚膜、菌毛、脂多糖、外膜蛋白及酶类等。笔者对这些毒力因子及该病的致病机理进行综述,以期为该病的防控提供科学依据。 相似文献
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以HPS的16SrRNA中的基因序列设计合成引物，进行PCR扩增，标准菌株NR4、SW124、SW114、SW140和分离株XX、FS在预计的821bp位置上扩增出特异性条带，NJING株、胸膜肺炎（APP）等没有特异性条带产生。这证明PCR检测副猪嗜血杆菌具有较高的特异性和敏感性。 相似文献
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Michael Carnevale Jeryl Jones Ida Holskov D. Phillip Sponenberg 《Veterinary radiology & ultrasound》2019,60(4):378-389
Degenerative sacroiliac joint disease is a cause of lumbosacral pain in dogs; however, published information on cross‐sectional imaging characteristics is limited. Objectives of this retrospective, secondary analysis, methods‐comparison study were to test hypotheses that CT lesions reported in humans with degenerative sacroiliac joint disease are also present in dogs, and that CT is comparable to gross pathology for detecting these lesions. Matched CT and gross pathology slice images of 30 sacroiliac joints were retrieved from a previous prospective, canine cadaver study. A veterinary radiologist interpreted randomized CT images for each joint based on previously published CT characteristics of lesions in humans with degenerative sacroiliac joint disease. A veterinary pathologist independently interpreted randomized gross pathology images using the same criteria. All joints had at least one CT lesion consistent with degenerative sacroiliac joint disease. A new CT lesion was also identified and termed “subarticular cleft.” The CT and gross pathology methods agreed for detecting joints with subchondral sclerosis, subchondral erosion, and intra‐articular ankylosis lesions (P > .05, McNemar's test), but disagreed for detection of joints with subchondral cyst, para‐articular ankylosis, and subarticular cleft lesions (P ≤ .05). Using gross pathology as the reference standard, CT had 100% sensitivity for detection of subarticular cleft and subchondral cyst lesions, with 56% and 22% specificity, respectively. Para‐articular ankylosis lesions were detected by CT but not by gross pathology. Findings supported the hypothesis that CT lesions reported in humans with degenerative sacroiliac joint disease are also present in dogs, and partially supported the hypothesis that CT is comparable to gross pathology for detecting joints with these lesions. 相似文献
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Magori E Nakamura M Inoue A Tanaka A Sasaki N Fukuda H Mizutani H Sako T Kimura N Arai T 《Research in veterinary science》2005,78(1):39-44
The activities of the enzymes in the malate-aspartate shuttle were measured in peripheral leucocytes of spontaneous type 1 diabetic dogs and cats treated with insulin injections. In the diabetic dogs and cats, fasting plasma glucose concentrations were three- or fourfold greater than the control levels in spite of insulin injections and the activities of cytosolic malate dehydrogenase (MDH), one of pivotal enzymes in the malate-aspartate shuttle, were remarkably lower than the controls. Depressed expression of cytosolic MDH mRNA was confirmed by RT-PCR analysis in the diabetic animals. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity (M / L ratio) in leucocytes of the diabetic animals was significantly lower than that of normal control animals. The smaller M / L ratio appeared to reflect depression of energy metabolism in the diabetic animals. Intrinsically lower and further decreased MDH activities may be factors that induce insulin resistance observed in diabetic cats. 相似文献
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使它隆对几种草坪草生理特性的影响 总被引：4，自引：0，他引：4
通过对狗牙根Cynodon dactylon,杂交狗牙根C.dactylon ×C. transvaalensis品种Tifgreen(328)、Tifway(419)、Tifdwaf以及海雀稗Paspalum vaginatum,两耳草P.conjugatum,百喜草P.notatum,地毯草Axonopus compressus,类地毯草A.affinis,金叶钝叶草Stenotaphrum secundatum,竹节草Chrysopogon aciculatus,假俭草Eremochloa ophiuroides,匍匐剪股颖粤选1号Agrostis stolonifera cv. Yuexuan No.1新品系叶面喷施使它隆溶液,测定了部分生理指标.结果表明,使它隆对百喜草安全,0.1%使它隆溶液可用于狗牙根、海雀稗、两耳草、金叶钝叶草及假俭草草坪;高浓度使它隆总体上使草坪草叶片叶绿素含量降低、膜透性增加、可溶性糖和可溶性蛋白含量增加. 相似文献
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JONES LM 《The Cornell veterinarian》1956,46(3):382-396
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抗菌肽以其优越的特点,如具有广谱抗菌活性,选择性杀灭病原体,水溶性好和耐热性能高等,在渔业养殖上有取代抗生素的趋势。文章综述几种鱼类抗菌肽的种类及其生物学活性、抗菌机制、抗菌肽基因的表达调控以及一些分子生物手段在此方面的研究应用,并做出展望。 相似文献
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