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1.
Some recalcitrant organic wastes, which contain a large proportion of lignin or cellulose, are not changed much by composting, and thus the effectiveness of the compost as fertilizer is usua.lly low. In this study, incubation of unripe compost with ligno-cellulolytic microorganisms —Trichoderma viride orBacillus spp. — was investigated to increase the degree of humification of the organic matter present, and improve ils quality as a soil amendment. High-performance liquid chromatography (HPLC) analyses together with humification indices and electrofocusing patterns were used to monitor the evolution of the humic substances during the incubation process. Plant growth effects exerted by Azotobacter chroococcum on lettuce plants growing on the previously incubated compost were affected by the length of incubation and by changes in the composition of humic substances. Higher organic matter content and better humification seem to be important factors for predictingA. chroococcum hebaviour in the rhizosphere.  相似文献   

2.
The goal of this experiment was to investigate the effect of aeration quantity (0, 11, 33, 55, and 77 L·min?1) on the growth of aerated compost extracts from a pig manure–straw compost. When the aeration quantity was 11 L·min?1, lettuce root growth enhancement of normalized compost extracts was at a maximum. As the aeration quantity increased, the total water-soluble organic carbon (TWSOC), total nitrogen (TN), total phosphorus (TP), humic carbon (humic C) content, and humification degree of compost extracts improved gradually. No differences in functional group structure were found among the aerated compost extracts. The positive root growth could be attributed to physicochemical and spectral characteristics, such as TN content, humic substances content, humification, aromaticity, and the low content of carboxyl groups. In conclusion, the aeration quantity of 11 L·min?1 was suitable for the production of aerated compost extracts, which obtained much greater promotion growth.  相似文献   

3.
The fate of organic matter during composting is poorly understood. Therefore, we analysed composts of sewage sludges and green wastes (44 samples representative of 11 stages of biodegradation) by conventional chemical methods: pH, humic (HA) and fulvic acid (FA) content, C, N and organic matter (OM) content, and by 13C CPMAS NMR to assess the decomposition process of the organic matter. Chemical changes clearly occurred in two phases: first, decomposition of OM during the first 2 months was characterized by decreased C/N ratios, OM content and increased pH; and second, a humification process with increased HA/FA ratios. NMR spectrum changes confirmed this pattern, with an increase in aromaticity and a decrease in alkyl C. A decrease of syringyl to guaiacyl ratio (S/G), a sign of lignin transformation, also indicated humification during composting. NMR spectroscopic properties of composts were also studied by means of principal components analysis (PCA) and revealed changes according to the degree of compost maturation. The factorial map presents a chronological distribution of composts on the two first principal components. The influences of eight chemical factors on the PCA ordination of composts as monitored by their evolution by NMR were also studied by multivariate analyses. PCA clearly indicated two phases: the rapid decomposition of organic matter followed by the formation of humic‐like substances. The first phase, that is ‘new’ composts, was strongly correlated with OM contents, pH and C/N ratios whereas the second phase, corresponding to ‘old’ compost, was correlated with pH, HA content and HA/FA ratio. These results confirm that knowledge of the formation of humic substances is indispensable to suitable monitoring of the composting process.  相似文献   

4.
隋宗明  殷洁  李轩  袁玲 《土壤》2017,49(3):527-533
为紫茎泽兰是我国危害最严重的外来入侵植物,具有生物毒性,无害化处理与资源化利用可经济、有效地防除紫茎泽兰。试验检测了紫茎泽兰生物堆肥的质量,并将紫茎泽兰生物堆肥(M)、化肥(CF)和生物堆肥+化肥(CFM)分别施入土壤,利用培养试验比较了它们对土壤有机质、养分和微生物的影响,为客观评价紫茎泽兰生物堆肥质量、微生物毒性和肥效提供有益信息。结果表明,紫茎泽兰生物堆肥质量符合NY525-2011国家标准(水分除外),且优于自然堆肥。在120 d的培养期间,CF处理降低土壤真菌和放线菌数量,对细菌数量无显著影响;CFM和M处理可提高土壤有机质和微生物量碳、氮,增加土壤细菌、真菌和放线菌数量。在添加化肥的土壤中,培养前期的碱解氮、脲酶和蔗糖酶活性高于CK(不施肥),后期与CK无显著差异。在CFM和M处理的土壤中,碱解氮变化平缓,但持续高于CK,蔗糖酶、脲酶、酸性磷酸酶和过氧化氢酶活性显著高于CF和CK。因此,紫茎泽兰生物堆肥对土壤有机质、氮、微生物生物量及土壤酶活性的影响类似于普通有机肥。  相似文献   

5.
The development of humic substances in the course of four composting processes was monitored quantitatively, recording both relative and absolute contents. Relative data showed contrasting results if the humic substances (HS) were related to the dry matter (d.m.) or to volatile solids (VS). Humic substances were apparently formed because of a concentration effect due to organic matter degradation. If absolute contents were considered, a decrease in the humic substances was observed, above all in the early stages of the process, due, probably, to degradation of the organic material, such as proteins, carbohydrates and lipids, coextracted with the humic substances. Processing of the data in respect of humic substance content over 13 composting processes and one study on the degradation of plant residues in soils, confirmed that no net humic substances are formed during composting and that the humification should be interpreted merely as degradation of the organic matter associated with the humic substances, after uncovering what is known as the core of the humus. The concept of humification during the composting process therefore needs to be reviewed, bearing in mind that neither the method commonly used for humic substance extraction nor the relative results obtained enable it to be interpreted satisfactorily.  相似文献   

6.
Two commonly-used composts from dairy cow manure that are used to improve poor structure and fertility of desert soils have inhibitory effects on wheat seed germination, probably as a result of their high levels of humic acids. Inoculation of wheat seeds with two species of the plant growth-promoting bacteria Azospirillum brasilense Cd and A. lipoferum JA4 (separately) prior to sowing in these amended soils improved germination, similar to the natural level of germination of seeds in desert soil without compost amendment. Both compost amendments increased height of wheat seedlings in the range of 20–25%, increased shoot dry weight by 15–19%, but severely decreased (51–54% less) root dry weight. Inoculation of wheat seeds with A. brasilense Cd, but not with A. lipoferum JA4, significantly increased plant growth parameters (height, shoot and root dry weight) over control plants grown in soil-compost mixtures. This bacterial species could survive for a period of 20 days in compost humic acid solution, could increase its population when the humic acids served as the sole carbon source, and may change the composition of humic acids in which it grows. We suggest that inoculation with A. brasilense may alleviate noxious effects on germinating seeds caused by compost application by possibly transforming the composition of humic acids in the compost.  相似文献   

7.
Organic wastes such as sewage sludge and compost increase the input of carbon and nutrients to the soil. However, sewage sludge-applied heavy metals, and organic pollutants adversely affect soil biochemical properties. Therefore, an incubation experiment lasting 90 days was carried out to evaluate the effect of the addition of two sources of organic C: sewage sludge or composted turf and plant residues to a calcareous soil at three rates (15, 45, and 90 t of dry matter ha–1) on pH, EC, dissolved organic C, humic substances C, organic matter mineralization, microbial biomass C, and metabolic quotient. The mobile fraction of heavy metals (Zn, Cd, Cu, Ni, and Pb) extracted by NH4NO3 was also investigated.The addition of sewage sludge decreased soil pH and increased soil salinity to a greater extent than the addition of compost. Both sewage sludge and compost increased significantly the values of the cumulative C mineralized, dissolved organic C, humic and fulvic acid C, microbial biomass C, and metabolic quotient (qCO2), especially with increasing application rate. Compared to compost, the addition of sewage sludge caused higher increases in the values of these parameters. The values of dissolved organic C, fulvic acid C, microbial biomass C, metabolic quotient, and C/N ratio tended to decrease with time. The soil treated with sewage sludge showed a significant increase in the mobile fractions of Zn, Cd, Cu, and Ni and a significant decrease in the mobile fraction of Pb compared to control. The high application rate of compost resulted in the lowest mobility of Cu, Ni, and Pb. The results suggest that biochemical properties of calcareous soil can be enhanced by both organic wastes. But, the high salinity and extractability of heavy metals, due to the addition of sewage sludge, may limit the application of sewage sludge.  相似文献   

8.
Two types of compost, consisting of sweet sorghum bagasse with either sewage sludge or a mixture of pig slurry and poultry manure, were studied in a pilot plant using the Rutgers system. The total degradation of the piles as determined by the weight loss of organic matter during the bio-oxidative and maturation phases accounted for 64% of the organic matter applied and followed a first-order kinetic function. Concentrations of total and organic N increased during the composting process as the degradation of organic C compounds reduced the compost weight. Losses of N through NH3 volatilization were low, particularly in the compost with sewage sludge due to pH values of <7.0 and the low temperatures reached in the compost during the first 2 weeks. The C:N ratio in the two composts decreased from 24.0 and 15.4 to values between 12 and 10. Increases in cation exchange capacity and in fulvic and humic acid-like C revealed that the organic matter had been humified during composting. The humification index, the C:N ratio, fulvic:humic acid-like C, and cation exchange capacity proved to be the most suitable parameters for assessing the maturity of these composts.  相似文献   

9.
Humic substances improve the efficiency of different iron (Fe) sources overcoming Fe deficiency chlorosis of plants. However, applied at high rates, they can promote negative effects on plants. The main objective of this work was to study the potential adverse effect of three humic acids from different origin when they were applied with two effective Fe sources for plants: Fe- ethylenediaminedihydroxyphenylacetic acid (EDDHA) and Vivianite. To this end, an experiment with lupin (Lupinus albus L.) was performed involving two factors: (i) Fe source, and (ii) humic substances from three different origin (composted cork, leonardite, and compost obtained from a mixture of olive husk with cotton gin trash) applied at 0, 0.1, and 0.5 g organic carbon (C) kg?1 of growing media. At the rates used, humic substances promoted adverse effects on plant development, chlorophyll meter readings, and Fe content in lupin grown in calcareous media. Overall, the effect on dry matter and Fe content in plants was more relevant when Fe was supplied with Vivianite, the effect on chlorophyll meter readings being more significant when Fe was applied as Fe-EDDHA. Differences were also observed depending on the source of humic substances, those from leonardite promoting the greatest decrease in dry matter in roots and shoots. These humic substances possessed the highest values of spectroscopy index for aromaticity (A254 ). On the other hand, the application of humic substances from olive husk compost, which exhibited the lower aromaticity index, resulted in the smallest decrease in dry matter production and chlorophyll meter readings. Dry matter in roots decreased logarithmically with increased values of the estimates of the amounts of aromatic compounds accumulated in the growing media (R2 = 0.92; P < 0.01) with Vivianite as Fe source. Thus, the effects decreasing dry matter production, particularly in roots, and chlorophyll meter readings can be ascribed at least partially to the presence of phytotoxic aromatic compounds in humic substances.  相似文献   

10.
A range of low-molecular-weight organic acids were identified in rhizosphere soil, leaf litter, and poultry manure compost. Laboratory and greenhouse experiments were carried out to examine the effects of seven low-molecular-weight organic acids on phosphate adsorption by soils, and the solubilization and plant uptake of P from soil pre-incubated with monocalcium phosphate and North Carolina phosphate rock. Acetic, formic, lactic (monocarboxylic), malic, tartaric, oxalic (dicarboxylic), and citric (tricarboxylic) acids were used in the study. The addition of organic acids decreased the adsorption of P by soils in the order tricarboxylic acid>dicarboxylic acid>monocarboxylic acid. The decreases in P adsorption with organic acid addition increased with an increase in the stability constant of the organic acid for Al (logK Al). Organic acids extracted greater amounts of P from soils meubated with both monocalcium phosphate and phosphate rock than water did. Although more phosphate was extracted by the organic acids from monocalcium phosphate — than from phosphate rock — treated soils in absolute terms, when the results were expressed as a percentage of dissolved phosphate there was little difference between the two fertilizers. The amount of P extracted by the organic acids from both fertilizers increased with an increase in logK Al values. The addition of oxalic and citric acids increased the dry matter yield of ryegrass and the uptake of P in soils treated with both fertilizers. The agronomic effectiveness of both fertilizers increased in the presence of organic acids and the increase was greater with the phosphate rock than with the monocalcium phosphate. The results indicated that organic acids increase the availability of P in soils mainly through both decreased adsorption of P and increased solubilization of P compounds.  相似文献   

11.
Summary Isoelectric focusing was used to characterize the organic matter of composted and uncomposted sewage sludge. The technique was applied to organic matter extracts and to three fractions, obtained by ultrafiltration, with different molecular weights (<103, 103–104, >104). The elution curves of the extracts through Sephadex G-50 revealed a loss in the proportion of organic matter of low molecular weight as composting progressed, together with an enrichment of the high-molecular-weight proportion. Separation into fractions by controlled ultrafiltration proved to be valid, as deduced from the chromatograms obtained by Sephadex G-50 filtration. The extracts of uncomposted sludge showed a greater number of bands with a low isoelectric point than the composted extract, because there were more acidic molecules in the samples that had not undergone humification. The spectrum corresponding to the extract of the 210-day compost showed greater homogeneity with a lower number of bands. A great part of the organic matter extracted with 0.1 M Na4P2O7 at pH 7.1 corresponded to an intermediate molecular weight. The ampholytes at pH 4–6 gave better resolution than those at pH 3.5–10, in the focusing of fractions with the lowest and the greatest molecular weight. A more homogeneous spectrum was observed for the high-molecular-weight fractions from extracts of the 210-day composted sample; in addition, the bands were displaced towards higher isoelectric points, which indicated that the molecules were more condensed, with a minor content of negatively charged groups and a spectrum similar to that of relative fractions of true humic acids.  相似文献   

12.
Parallel experiments were carried out to evaluate the induction of genotoxic effects in Vicia faba seedlings treated both with aqueous and organic extracts (dimethyl sulphoxide 5%) of 13 composts, or grown on soil samples to which different doses of the same composts were added. Pearsons linear correlation coefficient and multiple regression were used to study how these two different methodological approaches (treatment with the extracts versus direct contact with the composts in soil) agree. In addition, multiple regression analysis was used to investigate the role of heavy metals and humic substances in genotoxic effects and to construct a model to be used to accurately predict potential genotoxicity of the composts. Experimental data obtained at the highest application rate of compost on soil were found to fit the model and evidenced the role of the humic substances in modulating the potential genotoxicity of the different composts.  相似文献   

13.
A screening assay for isolating humic-substances degrading fungi from biosolids compost at the thermophillic phase employed using plates containing 2,2′-azino-bis(3-ethylbenzothiazoline-sulfonic acid) (ABTS), MnCl2 or the monoazo dye Acid Red 183. Two of the most active fungi out of 70 fungal strains isolated were identified based on rDNA sequences and designated Trametes sp. M23 and Phanerochaete sp. Y6 (accessions no. DQ408582 and DQ438910). These isolates, when compared to a model white-rot fungi T. versicolor and P. chrysosporium, showed the ability to bleach humic acids extracted from biosolids compost while growing under solid-state conditions using perlite as a solid support. T. versicolor and Trametes sp. M23 also exhibited the ability to bleach humic acids from a peat source. Interestingly, only Trametes sp. M23 bleached leonardite humic acid, which is considered to be a highly aromatic and stable type of natural organic matter. To the best of our knowledge, this is the first report of white-rot fungi being isolated and identified from thermophilic composts. Since these fungi are capable of degrading lignin and humic acid and were found active in organic-matter-degradation processes, we suggest that they may play a significant role in the degradation and transformation of these refractory substances during composting.  相似文献   

14.
Microbial communities are responsible for soil organic matter cycling and thus for maintaining soil fertility. A typical Orthic Luvisol was freed from organic carbon by thermal destruction at 600°C. Then the degradation and humification of 14C‐labelled maize straw by defined microbial communities was analysed. To study the role of microbial diversity on the humification of plant material, microcosms containing sterilized soil were inoculated with a natural microbial community or with microbial consortia consisting of bacterial and fungal soil isolates. Within 6 weeks, 41 ± 4% of applied 14C‐labelled maize straw was mineralized in the soil microcosms containing complex communities derived from a soil suspension, whilst the most efficient communities composed of soil isolates mineralized less than 35%. The humification products were analysed by solution state 13C‐NMR‐spectroscopy and gel permeation chromatography (GPC). The analyses of humic acids extracts by solution state 13C‐NMR‐spectroscopy revealed no difference in the development of typical chemical functional groups for humic substances during incubation. However, the increase in specific molecular size fractions of the extracted humic acids occurred only after inoculation with complex communities, but not with defined isolates. While it seems to be true that redundancy in soil microbial communities contributes to the resilience of soils, specific soil functions may no longer be performed if a microbial community is harshly affected in its diversity or growth conditions.  相似文献   

15.
Summary Leaf explants of Nicotiana plumbaginifolia were compared in cultures supplemented with hormones or humic substances (extracted from faeces of Allolobophora caliginosa) of various molecular complexity and concentration. The results showed that the humic substances (F1, F2, and T) at the concentration of 1 mg C l-1 produced greater leaf explants than those grown in the control. Furthermore, humic fractions like gibberellic alone induced a rhizogenic activity in leaf explants. Quantitative differences were also observed in the peroxidase activity induced in Nicotiana plumbaginifolia by humic matter (F2, F2, and T). In addition, the Nicotiana sp. tissue treated with humic fractions revealed, in the esterase enzyme pattern, the appearance of the 2a band, which was attributable to indoleacetic acid, since its profile was consistent with those obtained from tissues treated with indoleacetic acid. These differences demonstrate that humic substances exhibited a hormone-like behaviour, but no evidence of a relationship between biological activities and chemical characteristics of humus substances was found.  相似文献   

16.
Abstract

The cation exchange capacity (CEC) and content of COOH groups in bulky water hyacinth compost were estimated over the rotting period. The progressive increase observed in the estimates was ascribed to continuing oxidation processes in the composted materials.

Fractionation of organic carbon extracted with Na4P2O7-NaOH from the composted materials over the rotting period on the basis of acid-soluble and -insoluble fractions, was carried out. The carbon of humic-like substances (precipitated at pH 2) in the dry matter was almost stable from 0- to 124-day composted materials. There were successive increases in total acidity, and content of carboxyl and phenolic hydroxyl groups in the humic substance extracts indicating progressive oxidation processes in these substances over the rotting period. Elementary analysis and IR spectra provided fürther support for the above inference and led us to conclude that humification of the water hyacinth consists mainly of structural modification of humic-like substances initially occurring rather than a condensation of simple organic carbon to form high molecular weight material. Such structural modification is dominated by biochemical oxidation and increases in oxygen-containing functional groups. This conclusion is consistent with the view that “humic acids are formed by processes involving oxidation of plant lignin and lignoprotein entering the soil.”  相似文献   

17.
The objective of this study was to investigate differences in organic matter fractions, such as dissolved organic carbon and humic substances, in soils under different land uses. Soil samples were collected from the upper layer of arable lands and grasslands. Humic substances (HS) were chemically fractionated into fulvic acids (FA), humic acids (HA) and humins (HUM), and based on the separated fractions, the humification index (HI) and the degree of HS transformation (DT) were calculated. Dissolved organic carbon (DOC) was determined by cold (CWE) and hot water (HWE) extractions. Regardless of land use, the results indicated significant differences in soil organic carbon (SOC) and HS composition, with HA and HUM as the dominant fractions. Total SOC was higher in grassland (median = 17.51 g kg?1) than arable soils (median = 9.98 g kg?1); the HI and DT indices did not differ significantly between land uses (HI = 0.3–10.3 and DT = 0.2–6.2 for grasslands, p > 0.05; HI = 0.3–3.9 and DT = 0.2–20.1 for arable lands, p > 0.05). This indicates the relatively high stability of organic carbon and efficient humification processes in both land uses. Additionally, in arable soils lower CWE‐C (0.75 g kg?1) and higher HWE‐C (2.59 g kg?1) than in grasslands (CWE‐C = 1.13 g kg?1, HWE‐C = 1.60 g kg?1) can be related to farming practice and application of soil amendments. The results showed that both labile and humified organic matter are better protected in grassland soils and are consequently less vulnerable to mineralization.  相似文献   

18.
To characterize the nature of humus in paddy soils, a comparison was made between the properties of humus in paddy soils and those in adjacent unflooded arable soils.

Rice cultivation generally brought about a considerable increase in organic matter and in the PQ-value, with the exception of Andosol-paddy soils in which organic matter tended to decrease somewhat and the PQ-value remained virtually unchanged. The humification degree of humic acid as judged from Δ log k and RF values was generally lowered by rice cultivation except in the case of Yellow soil-paddy soil in which humic acid was originally low in the degree of humification.

The accumulation of poorly humified humic acid may be a characteristic feature common to all paddy soils. These changes by rice cultivation are observed only in the upper part of the profiles, and seem to be associated with seasonally flooded conditions ot paddy soils. Iron oxides accumulated in subsurface soil have virtually no effect on the properties of humus.  相似文献   

19.
Strongly reducing organic substances (SROS) and iron oxides exist widely in soils and sediments and have been implicated in many soil and sediment processes. In the present work, the sorptive interaction between goethite and SROS derived from anaerobic decomposition of green manures was investigated by differential pulse voltammetry (DPV). Both green manures, Astragalus sinicus (Astragalus) and Vicia varia (Vicia) were chosen to be anaerobically decomposed by the mixed microorganisms isolated from paddy soils for 30 d to prepare different SROS. Goethite used in experiments was synthesized in laboratory. The anaerobic incubation solutions from green manures at different incubation time were arranged to react with goethite, in which SROS concentration and Fe(II) species were analyzed. The anaerobic decomposition of Astragalus generally produced SROS more in amount but weaker in reducibility than that of Vicia in the same incubation time. The available SROS from Astragalus that could interact with goethite was 0.69 ± 0.04, 0.84 ± 0.04 and 1.09 ± 0.03 cmol kg−1 as incubated for 10, 15 and 30 d, respectively, for Vicia, it was 0.12 ± 0.03, 0.46 ± 0.02 and 0.70 ± 0.02 cmol kg−1. One of the fates of SROS as they interacted with goethite was oxidation. The amounts of oxidizable SROS from Astragalus decreased over increasing incubation time from 0.51 ± 0.05 cmol kg−1 at day 10 to 0.39 ± 0.04 cmol kg−1 at day 30, but for Vicia, it increased with the highest reaching to 0.58 ± 0.04 cmol kg−1 at day 30. Another fate of these substances was sorption by goethite. The SROS from Astragalus were sorbed more readily than those from Vicia, and closely depended upon the incubation time, whereas for those from Vicia, the corresponding values were remarkably less and apparently unchangeable with incubation time. The extent of goethite dissolution induced by the anaerobic solution from Vicia was greater than that from Astragalus, showing its higher reactivity.  相似文献   

20.
To determine whether there is a relationship between the composition of soil organic matter and the activity of the soil microbial biomass, the composition of the organic matter in 12 typical arable soils in Northwest Germany was investigated by wet chemical analysis and CPMAS cross polarization magic angle spinning 13C-NMR spectroscopy. The data were correlated with the microbial biomass as estimated by substrate-induced respiration. A strong correlation between the microbial biomass and alkylic C compounds was observed (r=-0.960***). Recalcitrant substances were enriched in this fraction, which were classified as humic acids according to the wet chemical procedure. The microbial decomposition of these humic acids is probably retarded, due to their chemical structure and/or physical bonding, when the soil microbial biomass activity is limited.  相似文献   

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