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1.
L-精氨酸对脂多糖刺激断奶仔猪肠黏膜免疫屏障的影响 总被引：1，自引：0，他引：1
本试验旨在研究L-精氨酸(L-Arg)对脂多糖(LPS)刺激仔猪肠黏膜免疫的影响。选用24头21日龄断奶仔猪,随机分为4个处理,即对照组(基础日粮)、LPS组(基础日粮+LPS)、0.5%Arg组(基础日粮添加0.5%Arg+LPS)、1.0%Arg组(基础日粮添加1.0%Arg+LPS)。结果表明:LPS刺激损伤小肠黏膜结构;Arg则有效阻止LPS应激导致的肠道损伤,维持肠黏膜结构的完整性;LPS刺激显著增加仔猪小肠肥大细胞数(P<0.01);1.0%Arg则减少肥大细胞数(P<0.05);LPS刺激降低回肠IgA分泌细胞、CD4+、CD8+阳性细胞数(P<0.05),而0.5%Arg则增加IgA分泌细胞、CD4+、CD8+阳性细胞数(P<0.05)。这表明LPS刺激可导致仔猪小肠黏膜结构受损,免疫屏障功能降低,而日粮添加精氨酸可有效阻止LPS对肠黏膜屏障的损伤,促进及改善肠黏膜结构及免疫屏障功能。 相似文献
2.
L-精氨酸对脂多糖刺激断奶仔猪生产性能、血液生化指标和内脏器官重量的影响 总被引：2，自引：0，他引：2
试验研究L-精氨酸(Arg)对脂多糖(LPS)刺激断奶仔猪[(21±1)d,平均体重(5.78±0.26)kg]生产性能、血液生化指标和内脏器官重量的影响。试验采用单因子设计,分为以下4个处理组:(1)基础日粮(非应激对照组);(2)基础日粮 LPS(应激对照组);(3)基础日粮 LPS 0.5%Arg(0.5%Arg组);(4)基础日粮 LPS 1.0%Arg(1.0%Arg组)。在试验第16天,给处理2、处理3和处理4组仔猪按每千克体重注射100μgLPS,处理1组注射等量的生理盐水,3h后采血。试验第18天屠宰仔猪测定内脏器官的重量。结果表明:(1)LPS刺激导致断奶仔猪日增重和日采食量显著下降(P<0.001),而0.5%Arg可缓解LPS刺激所导致的日增重下降(P<0.001);(2)LPS刺激导致断奶仔猪血浆葡萄糖、总蛋白、白蛋白和球蛋白含量显著降低(P<0.05),而0.5%或1.0%Arg可在一定程度上缓解LPS刺激导致的血浆总蛋白、白蛋白和球蛋白含量降低;(3)LPS刺激导致胃绝对重量显著降低(P<0.05)和肝脏相对重量增加(P<0.05),0.5%或1.0%Arg可在一定程度上缓解LPS刺激导致的胃绝对重量的减轻和肝脏相对重量的增加。这表明Arg在一定程度上缓解了LPS刺激所导致生长抑制和应激反应。 相似文献
3.
L-精氨酸对脂多糖刺激的断奶仔猪肠道损伤的缓解作用 总被引：2，自引：1，他引：2
研究L-精氨酸(Arg)对脂多糖(LPS)刺激的断奶仔猪小肠黏膜蛋白质、DNA、RNA含量、二糖酶活性和血浆D-木糖含量的影响,旨在探讨L-Arg是否可以缓解LPS刺激所导致的肠道损伤.选取24头健康的(21±1)d断奶仔猪,分成4个处理,每个处理6个重复.采用单因子设计(1)基础日粮(非应激对照组);(2)基础日粮 LPS(应激对照组);(3)基础日粮 LPS 0.5%Arg(0.5%Arg组);(4)基础日粮 LPS 1.0%Arg(1.0%Arg组).在第16天,给应激对照组、0.5%和1.0%Arg组注射100 μg/kg BW的LPS,非应激对照组注射等量的生理盐水.注射LPS 2 h后,按1 mL/kg BW的剂量分别给仔猪灌服D-木糖溶液,注射LPS 3 h后,采血,分离血浆待测;注射LPS后48 h屠宰仔猪,刮取小肠黏膜待测.结果表明(1)和非应激对照组相比,应激对照组的十二指肠黏膜蛋白质含量,空肠、回肠黏膜DNA含量,空肠黏膜蔗糖酶活性,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量均显著降低(P＜0.05),这表明LPS刺激导致断奶仔猪肠道损伤;(2)0.5%Arg组十二指肠黏膜蛋白质含量,空肠黏膜DNA含量,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量以及1.0%Arg组空肠DNA含量,空肠黏膜蔗糖酶活性,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量均高于应激对照组,且与非应激对照组无显著差异(P＞0.05).这表明L-Arg在一定程度上缓解了LPS刺激所造成的肠道损伤. 相似文献
4.
L-精氨酸对脂多糖刺激仔鹅生长发育的影响 总被引：1，自引：0，他引：1
本试验旨在研究脂多糖(LPS)刺激所产生的免疫应激对仔鹅早期生长发育的影响及L-精氨酸对免疫应激的缓解作用.选取128只14日龄健康扬州鹅公鹅,随机分为A、B、C、D 4个组,每组4个重复,每个重复8只鹅.A组饲喂基础饲粮,并静脉注射生理盐水;B组饲喂基础饲粮,并注射细菌LPS;C组饲喂添加0.5%L-精氨酸的基础饲粮,并注射LPS;D组饲喂添加1.0%L-精氨酸的基础饲粮,并注射LPS.14日龄逐一称重,并分别于15、17、19日龄进行注射,21日龄时屠宰采样,称量试验鹅活重以及心脏、肝脏、肌胃、脾脏、胸腺、腔上囊的重量.结果表明:1)LPs刺激导致3周龄仔鹅体增重显著下降(P<0.05),而0.5%精氨酸可在一定程度上缓解LPS刺激导致的3周龄仔鹅体增重的下降(P>0.05);2)LPS刺激导致3周龄仔鹅腔上囊重量显著降低(P<0.05),而0.5%精氨酸可在一定程度上缓解LPS刺激导致的腔上囊重量的降低(P>0.05);LPS刺激导致3周龄仔鹅腔上囊指数显著降低(P<0.05);0.5%或1.0%精氨酸使LPS刺激3周龄仔鹅的脾脏指数显著增加(P<0.05);3)LPS刺激导致3周龄仔鹅肠道长度显著减小(P<0.05),而0.5%精氨酸町在一定程度上缓解LPS刺激导致的肠道长度的减小(P>0.05).本试验以仔鹅生长发育为衡量指标,表明适当的精氨酸添加量可以缓解鹅由LPS刺激所产生的免疫应激. 相似文献
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《畜牧与兽医》2017,(4):40-45
旨在探讨精氨酸(Arg)或N-氨甲酰谷氨酸(NCG)对35 d哺乳期母獭兔繁殖性能及断奶幼兔生长性能和机体抗氧化能力的影响。选取哺乳期母兔45只,随机分为5组:基础日粮组(对照组)、基础日粮+0.3%Arg组(试验Ⅰ组)、基础日粮+0.6%Arg组(试验Ⅱ组)、基础日粮+0.03%NCG组(试验Ⅲ组)、基础日粮+0.06%NCG组(试验Ⅳ组),试验期35 d;选取35 d断奶体重相近健康幼兔100只,分为5个组,饲喂日粮和母兔组一致,试验期21 d。结果表明:试验Ⅱ、Ⅳ组母兔体重出现不同程度增长,再次受胎率Ⅱ组最好;哺乳仔兔21 d、35 d个体重,试验Ⅱ、Ⅳ组显著高于对照组(P0.05)。断奶幼兔试验中,各试验组末重、日增重均显著高于对照组(P0.05);试验Ⅱ、Ⅲ组的日采食量显著高于对照组(P0.05),但料重比无差异,试验Ⅰ、Ⅳ组日采食量、料重比均显著低于对照组(P0.05)。与对照组相比,哺乳35 d的各试验组仔兔血清抗氧化指标无显著差异(P0.05);而断奶幼兔在试验21 d时,试验Ⅲ、Ⅳ组血清SOD活力均显著高于对照组(P0.05)。结论:日粮添加精氨酸或NCG能维持母兔体重,提高哺乳仔兔个体重,并能提高断奶后幼兔个体重、平均日增重及降低料重比,且NCG能提高断奶后幼兔的抗氧化能力。 相似文献
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L-精氨酸对脂多糖诱导的断奶仔猪肠道黏膜屏障损伤的缓解作用 总被引：1，自引：0，他引：1
试验研究L-精氨酸(L-Arg)对脂多糖(LPS)诱导的断奶仔猪肠道黏膜屏障损伤的缓解作用.选择21日龄断奶的杜×长×大断奶仔猪18头,平均体重(7.52±0.92)kg,采用单因子试验设计,分为3个组:基础日粮(对照组)、基础日粮+LPS(LPS组)和基础日粮+LPS+0.5%Arg组(0.5%Arg组)(每组6个重复,每个重复1头猪).试验期为18 d.试验第18天,LPS组和0.5%Arg组腹腔注射100μg/kg BW LPS,对照组注射等量的生理盐水.注射LPS后4 h前腔静脉采血,屠宰,刮取小肠黏膜.结果表明:1)与对照组相比,LPS刺激显著降低十二指肠黏膜中二胺氧化酶(DAO)活性(P<0.05),与LPS组相比,0.5%Arg显著增加了十二指肠和回肠黏膜中DAO活性(P<0.05);2)与对照组相比,LPS对血浆D-乳酸含量无显著影响(P>0.05),而与LPS组相比,0.5%Arg显著降低了血浆中D-乳酸的含量(P<0.05);3)与对照组相比,LPS刺激显著增加了十二指肠黏膜中内皮素-1(ET-1)的含量(P<0.05).而与LPS组相比,0.5%Arg显著缓解了十二指肠黏膜中ET-1含量的上升(P<0.05);4)与对照组相比,LPS刺激显著增加了回肠黏膜中丙二醛(MDA)和空肠黏膜中还原型谷胱甘肽(GSH)的含量以及空肠黏膜中GSH与氧化型谷胱甘肽(GSSG)的比值(GSH/GSSG)(P<0.05),而与LPS组相比,0.5%Arg显著缓解了LPS导致的回肠黏膜MDA和空肠黏膜GSH含量的上升(P<0.05).这表明,L-Arg可通过降低肠黏膜中ET-1含量、降低机体的过氧化水平来缓解LPS导致的肠道黏膜屏障损伤. 相似文献
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试验研究了乙酰半胱氨酸(NAC)对脂多糖(LPS)多次刺激造成的免疫应激仔猪炎性介质的影响,旨在探讨NAC是否有缓解仔猪免疫应激的作用。选取来源一致、体重相近的18头健康仔猪[杜洛克×长白×大白,体重(11.58±0.26)kg],随机分成3个处理(对照组、LPS组和NAC组),每个处理6个重复,每个重复1头猪。对照组和LPS组饲喂基础日粮,试验组(NAC组)为基础日粮+500 mg/kg NAC,LPS组和NAC组仔猪分别于试验的第101、3、20 d腹膜注射100μg/kg BW的LPS,对照组注射等量的灭菌生理盐水。试验第10、20 d在注射LPS 3 h后前腔静脉采血,测定血浆TNF-α、CORI、L-6和PGE2含量。试验结果显示:日粮中添加NAC显著缓解了LPS刺激导致的血浆(第10 d)和血浆(第20 d)中TNF-α、CORI、L-6和PGE2含量的升高(P<0.05)。由此可见,在本试验条件下,LPS刺激引起仔猪血浆中炎性介质升高,日粮中添加500 mg/kg NAC缓解LPS导致的血浆中炎性介质的升高,缓解免疫应激,最终可能缓解LPS刺激引起的生长抑制。 相似文献
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精氨酸和精氨酸生素对断奶仔猪生长性能、器官重及生化指标的影响 总被引：3，自引：1，他引：2
试验选用相同胎次母猪的21日龄断奶杜洛克×长白×大约克健康仔猪120头,以栏为单位,依据仔猪栏平均体重(5.27 kg±0.63 kg)随机分为3组,每组5个重复,每个重复(栏)8头仔猪(公、母各半),分别饲喂对照日粮(基础日粮+2.05%丙氨酸)、精氨酸日粮(基础日粮+0.6%精氨酸)和精氨酸生素日粮(基础日粮+0.09% AAA),试验期为7 d,研究了日粮中添加精氨酸和精氨酸生素对断奶仔猪生长性能、器官相对重量和血液生化参数的影响。结果表明,日粮添加0.6%的精氨酸和0.09%的AAA,仔猪的平均日采食量、日增重有显著提高(P＜0.05);肝脏、肾脏、脾脏和心脏的重量高于对照组,但差异均不显著(P＞0.05);与对照组相比,精氨酸组和精氨酸生素组的血清尿素氮和碱性磷酸酶活性显著降低 (P＜0.05),其它血液生化参数差异都不显著(P＞0.05)。试验结果显示,日粮中添加精氨酸和精氨酸生素有利于缓解早期仔猪断奶应激,提高其生长性能,0.09%的AAA可以有效替代0.6%的精氨酸。 相似文献
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目的:试验目的是研究牡蛎粗多糖对脂多糖(LPS)刺激雄性大鼠产生的免疫应激是否产生缓解效果。方法:选择30只成年SD大鼠,按照体重靠近的原则,随机分成5个试验组(即空白对照组,免疫应激对照组,牡蛎粗多糖高剂量组、牡蛎粗多糖中剂量组、牡蛎粗多糖低剂量组),每组6只雄性大鼠。空白对照组和免疫应激对照组饲喂基础日粮,牡蛎粗多糖高、中、低剂量组为基础日粮添加0.9%、0.6%、0.3%牡蛎粗多糖。饲喂28 d后,免疫应激对照组、牡蛎粗多糖高、中、低剂量组按体重计算腹腔注LPS 100μg/kg,空白对照组则注射相等量的生理盐水。于注射3 h后采血,剖检取肝脏、脾脏、肾上腺、淋巴结,用RT-PCR检测TLR-4 m RNA的相对表达量。结果 :牡蛎粗多糖高剂量组、中剂量组、低剂量组和空白对照组肝脏、脾脏、肾上腺和淋巴结TLR-4 m RNA的相对表达量与应激对照组相比出现极显著性降低(P0.01),而牡蛎粗多糖中剂量组、高剂量TLR-4 m RNA的相对表达量较低剂量组更趋于生理状态下空白对照组的相对表达量。结论:LPS作为免疫原可以引起雄性大鼠产生免疫应激反应,在基础日粮中添加牡蛎粗多糖可以缓解免疫应激,且日粮中的添加量为0.6%~0.9%时,缓解效果较好。 相似文献
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选取24只28日龄健康扬州鹅公鹅,随机分为3组,每组4个重复。A、B组饲喂基础日粮并分别注射生理盐水、新城疫1(以下简称ND-1)系疫苗,C组饲喂添加0.5%精氨酸的基础日粮并注射ND-1系疫苗。试验期间于29和43日龄注射疫苗或生理盐水,旨在研究日粮添加精氨酸对免疫应激扬州鹅生长性能和内脏器官发育的影响。结果表明:C组鹅各个阶段的平均体增重均高于其他两组,而饲料增重比低于其他2组(P>0.05);C组鹅十二指肠长度显著大于B组(P<0.05);ND-1系疫苗刺激导致鹅肝脏显著增大(P<0.05)。ND-1系疫苗刺激导致鹅的脾脏重量增加(P>0.05),而胸腺和法氏囊重量减小(P>0.05),饲料中添加0.5%的精氨酸对鹅血清新城疫抗体效价无显著影响。本试验表明,精氨酸在一定程度上缓解了免疫应激对鹅产生的不利影响。 相似文献
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REASONS FOR PERFORMING STUDY: Based on clinical observation, it is hypothesised that horses with duodenitis-proximal jejunitis (DPJ) that are treated surgically have a shorter duration, smaller volume, and slower rate of nasogastric reflux (NGR) compared to horses treated medically, are more likely to develop diarrhoea than medically managed cases, and have a higher incisional infection rate than a sample population of horses undergoing abdominal exploration for gastrointestinal disease other than DPJ. OBJECTIVES: To compare: 1) duration, volume and rate of NGR and the percentage of horses with diarrhoea between medically and surgically treated DPJ cases; and 2) incisional infection rate in horses with DPJ undergoing abdominal exploration to a sample population of horses undergoing abdominal exploration for gastrointestinal disease other than DPJ. METHODS: Medical records of cases with DPJ diagnosed 1995-2006 were reviewed. Information obtained included subject details, presenting clinical findings, treatment category (medical/surgical), complications (diarrhoea, incisional infection), and outcome (survival/nonsurvival). Data were analysed using a Chi-squared test and a mixed model analysis of variance. Level of significance was P<0.05. RESULTS: Compared to medical cases, surgical cases had significantly decreased survival, a longer duration and larger total volume of NGR, and were more likely to develop diarrhoea. The incisional infection rate for horses with DPJ undergoing abdominal exploration was 16% compared to 7% for the sample population of horses. CONCLUSIONS: Surgical treatment of horses with DPJ did not lead to resolution of NGR faster than medical treatment. Surgical cases were more likely to develop diarrhoea and did not have a significantly higher incisional infection rate than the sample population. 相似文献
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Kluger EK Malik R Ilkin WJ Snow D Sullivan DR Govendir M 《Journal of the American Veterinary Medical Association》2008,233(8):1270-1277
OBJECTIVE: To compare serum triglyceride concentrations obtained after food had been withheld (i.e., fasting concentrations) in dogs with epilepsy that had been treated long term (> or = 3 months) with phenobarbital or with phenobarbital and potassium bromide with concentrations in healthy control dogs. DESIGN: Cross-sectional study. ANIMALS: 57 epileptic dogs that had been treated with phenobarbital (n=28) or with phenobarbital and bromide (29) and 57 healthy, untreated control dogs matched on the basis of age, breed, sex, neuter status, and body condition score. PROCEDURES: Blood samples were collected after food had been withheld for at least 12 hours, and serum biochemical and lipid concentrations were determined. Oral fat tolerance tests were performed in 15 control dogs and 9 dogs with epilepsy treated with phenobarbital alone. RESULTS: 19 of the 57 (33%) epileptic dogs had fasting serum triglyceride concentrations greater than the upper reference limit. Nine (16%) dogs had a history of pancreatitis, and 5 of the 9 had high fasting serum triglyceride concentrations at the time of the study. A significant relationship was found between body condition score and fasting serum triglyceride concentration in all dogs, but serum triglyceride concentration was not significantly associated with phenobarbital dosage or serum phenobarbital concentration. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that dogs treated long term with phenobarbital or with phenobarbital and bromide may develop hypertriglyceridemia. Fasting serum triglyceride concentration should be periodically monitored in dogs treated with phenobarbital because hypertriglyceridemia is a risk factor for pancreatitis. 相似文献
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三月底一个风和日丽的日子,我们去镇江采访土著菌养鸡的情况。对于土著菌,我们并不佰生,因为,我们几年前就报道过镇江广电总局基地的土著菌养鸡。只是那时镇江的主要精力放在稻鸭共作的研究和推广上,这几年土著菌养鸡养猪才被越来越多的人重视起来。不但如此,镇江人又用推广稻鸭共作的热情推广起土著菌养鸡养猪来。 相似文献
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The pain-relieving effect of carprofen and tolerance to the drug were investigated in 805 dogs that were lame as a result of osteoarthritis. The dogs were of different breeds, ages and bodyweights and of both sexes, and were selected from 51 veterinary clinics. Each dog was treated orally by its owner with 4 mg/kg carprofen for 84 consecutive days. Twenty-four dogs were removed from the study because of side effects, and 55 left the study for reasons unrelated to the treatment. The condition of the dogs and the benefit of the treatment were evaluated by the veterinary surgeons and the owners after 14 days, and at the end of the period of treatment, when 194 of the dogs (26.7 per cent) were no longer lame, and 357 (49.2 per cent) had improved. The period for which the dogs had been lame before entering the study significantly (P<0.01) affected the results and the rate of improvement. Too much exercise during the 84 days of treatment caused some dogs to relapse. 相似文献
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Ohno K Konishi S Kobayashi S Nakashima K Setoguchi A Fujino Y Nakayama H Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(9):929-933
Prognostic factors associated with survival in dogs with lymphocytic-plasmacytic enteritis (LPE) were investigated through a retrospective study. Using case records, 48 dogs diagnosed with LPE were classified as survivors (n = 32) or non-survivors (n = 16), and the clinical and clinicopathological parameters were reviewed between the 2 groups by using univariate and multivariate prognostic analysis. Compared to the hospital population, non-survivors had an overrepresentation of the Shiba breed. Results of univariate analysis indicated that anorexia, severe weight loss, packed cell volume, and total protein were significantly associated with survival for 6 months after diagnosis. In multivariate analysis, anorexia and hypoproteinemia were significantly associated with survival. Furthermore, initial response to treatment was strongly associated with poor prognosis. Based on these clinical and laboratory parameters such as anorexia, hypoproteinemia and initial response to treatment, it may be possible to predict poor prognosis in canine LPE. 相似文献
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