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1.
番茄Hsp70基因鉴定及系统发育关系分析 总被引：1，自引：0，他引：1
热激蛋白70(heat shock protein 70,Hsp70)是植物应对高温和其他胁迫环境时所产生的一类特定的应激蛋白。本文以番茄基因组数据为基础,利用生物信息学方法对Hsp70基因家族进行鉴定与分析。结果表明,番茄至少含有22个Hsp70基因成员,蛋白质序列长度为210-890个氨基酸之间,具有0~12个内含子;具有4对重复基因;滑动窗口分析结果表明,这些基因中的一些区段(或者位点)可能受到正选择的压力。此外,序列比对发现这些Hsp70基因家族成员具有多个保守基序;染色体定位发现他们不均匀分布在番茄的11条染色体上;系统发育关系揭示番茄和拟南芥Hsp70基因家族成员可以分为4组,每组成员数目是变异的,并且存在3对旁系同源基因和7对直系同源基因,表明Hsp70基因家族在番茄和拟南芥分化之前就已经存在。 相似文献
2.
番茄SlMAPK9-2基因分离及表达分析 总被引：1，自引：0，他引：1
促分裂原活化蛋白激酶(MAPK)信号途径在植物生长发育以及多种逆境胁迫响应和激素调控过程中发挥着至关重要的作用。为了研究MAPK基因的结构和功能,利用生物信息学分析以及PCR扩增方法从番茄中分离了1个新的MAPK基因,克隆其c DNA全长序列,命名为Sl MAPK9-2;利用NCBI数据库Blast P工具和在线软件MEME分析发现其具有MAPK保守的结构域和保守基序。系统进化树分析显示Sl MAPK9-2属于D组MAPK,与茄科植物马铃薯和烟草MAPK9位于同一进化树分支。利用数据库PGDD分析发现,Sl MAPK9-2与Sl MAPK16以及Pm MAPK9之间发生了大片段复制事件,且2个基因对的Ka/Ks均小于1,说明经历了达尔文纯化选择。实时荧光定量PCR分析发现,Sl MAPK9-2在不同组织器官中均有表达,在开放花中表达量最高。非生物逆境(高温和盐胁迫)和外源激素(脱落酸和水杨酸)处理可以显著改变Sl MAPK9-2的表达水平。启动子预测分析也发现,Sl MAPK9-2启动子区含有大量响应病原菌、激素、高温及脱水的顺式作用元件。综上,Sl MAPK9-2可能在番茄逆境胁迫应答中发挥重要调控作用。本研究为进一步探索Sl MAPK9-2的生物学功能及作用机制奠定了基础。 相似文献
3.
GRX基因家族是从原核生物到真核生物中普遍存在的一类巯基-二硫键氧化还原酶,在植物的生长发育、器官构建及逆境胁迫和激素信号应答中均发挥重要作用.本研究在番茄基因组范围内,利用生物信息学方法对番茄的GRX基因组家族的成员、分布及结构和功能等进行分析.预测结果显示,番茄GRX家族包含55个蛋白质,分为4个亚族,其中植物特有的CC亚族成员最多,有35个,其他GRX基因成员与拟南芥GRX家族具有相似分类.在番茄GRX结构域中包含12个重要的基序,主要分布在序列的N端,相同亚族中的GRX成员蛋白序列的氨基酸保守域构成基本一致,且各亚族成员的氨基酸保守域组成特异,表明这些基序的存在对GRX蛋白功能的执行是必需的.利用实时荧光定量PCR对番茄GRX基因的组织表达和胁迫响应分析,结果表明,GRX基因具有组织特异性表达差异,CC型在根和花中表达较高,在果实中表达较低;在盐、SA、ABA、高温和低温胁迫条件下,22个番茄GRX基因的表达模式被阐明;其中部分基因的表达水平被显著地诱导增加或者降低,很可能参与了调控番茄逆境胁迫条件下的防御应答反应.本研究结果将为番茄GRX家族基因的深入研究提供依据,为进一步解析GRX基因功能奠定基础. 相似文献
4.
大豆（Glycine max）GmMYBZ2基因是典型的植物R2R3-MYB转录因子家族成员之一。通过DNAMAN和酵母(Saccharomyces cerevisiae)单杂交系统分别对其蛋白结构和转录活性进行了分析鉴定,并在大肠杆菌(Escherichia coli )中进行了原核表达。GmMYBZ2除含有典型的R2R3-MYB转录因子的结构特征外,在C端含有1个少有的保守氨基酸基序PDLNLELTIS及一个隐藏的锌指结构。基因组序列分析表明,在263～395 bp位含1个132 bp的内含子。为明确GmMYBZ2 C端保守氨基酸基序及锌指结构对目的蛋白转录活性的影响,分别进行了PCR介导的序列删除突变。酵母单杂交系统分析显示,内含子、保守基序及锌指结构对目的蛋白的转录活性均有抑制作用;原核表达显示,目的蛋白能在补充有稀有密码子的大肠杆菌中成功表达。 相似文献
5.
番茄KNOX基因家族鉴定及茄科作物KNOX基因进化关系分析 总被引：1，自引：0，他引：1
为了探明番茄KNOX基因家族特征及茄科作物中KNOX的进化关系,利用生物信息学方法,在全基因组水平上对番茄及其它茄科作物KNOX基因家族成员进行鉴定和分析。结果表明,番茄基因组中共含有8个KNOX基因(Sl KNOX1~Sl KNOX8),分别分布在番茄8条染色体上;多序列比对发现,除Sl KNOX5外,所有基因均含有同源异型盒蛋白特有的4种结构域,即KNOX1、KNOX2、ELK和Homeobox KN。利用系统发育树将茄科作物KNOX基因分为2类:ClassⅠ和ClassⅡ。茄科作物KNOX基因与拟南芥同源基因可能经历了不同的进化历程,在茄科作物中,特有的进化分支(Class I C)已经出现。qRTPCR分析发现,番茄Sl KNOX1在花蕾和花中具有较高表达,在果实生长发育过程中表达量逐渐降低,Sl KNOX2~Sl KNOX4在果实生长发育过程中几乎不表达,Sl KNOX6和Sl KNOX7在所测组织中均有表达,Sl KNOX8在果实成熟过程中表达量逐渐增加,且在根中表达量最大。本研究在全基因组水平上揭示了番茄中KNOX基因家族特征以及茄科作物中KNOX基因的系统发育关系,为进一步揭示KNOX基因家族在番茄组织器官中的功能及进化模式奠定了基础。 相似文献
6.
本研究中，通过隐马尔科夫模型（HMM）和杨树蛋白质库搜索，共找到17个杨树铵转运体蛋白（PtAMTs）。利用生物信息学方法，我们对杨树家族17条AMT蛋白序列的系统发生和AMT基因组定位进行分析，然后对其氨基酸组成成分、理化性质以及二级结构进行预测和分析，同时还分析了杨树与拟南芥、水稻、番茄、百脉根和欧洲油菜的AMT基因家族之间的联系。二级结构预测结果发现不同成员间氨基酸数目、氨基酸序列间的疏水性存在一定的差异；仪一螺旋和无规则卷曲为主要二级结构组成部分。同源性比对分析表明，PtAMT基因家族主要分为2个亚家族，AMT1（11个成员）和AMT2（6个成员），基因结果分析表明AMT2亚家族成员不含内含子。杨树AMT蛋白的亚细胞定位分析表明PtAMT主要定位于膜结构上。电子表达图谱分析结果表明：只有XP002309151和XP002334025基因有对应的EST序列，并有相应的电子表达谱，并主要在花蕾表达。 相似文献
7.
为探究过氧化物酶Ⅲ（peroxidase classⅢ，PERs）基因家族在药用植物响应电离辐射中的作用，本试验研究了中药材桔梗的PERs(PgPERs)家族成员特性，及桔梗幼苗经高能重离子束和X射线辐射处理后PgPERs基因的表达量变化。通过序列比对、结构域鉴定和蛋白保守基序分析，共获得53个PgPERs，其蛋白的序列长度多数为258～621个氨基酸，碱性蛋白占多数。启动子区调控元件分析结果说明PgPERs存在多个与生长发育、植物激素和逆境胁迫相关的顺式作用元件。进化分析结果显示，PgPERs基因家族主要分为5个簇，与拟南芥过氧化物酶Ⅲ（AtPERs）有29对共线性关系。高能重离子束辐射桔梗幼苗后检测到3个PgPERs基因表达量上调，X射线辐射处理后有8个PgPERs基因差异表达。这些差异基因编码的蛋白质二级结构以α-螺旋和无规则卷曲为主，三级结构的相似度高，并含有跨膜螺旋。以上结果表明，不同类型的电离辐射处理桔梗幼苗后，其PgPERs响应机制并不相同。本研究结果为探究电离辐射影响桔梗生长发育和辐射响应过程提供了新的视角。 相似文献
8.
为挖掘果梅中NAC基因成员,探讨其组织表达特异性,本研究采用生物信息学方法鉴定了果梅NAC基因家族,并对其理化性质、染色体分布、亚细胞定位、保守基序、蛋白结构和亚族分类进行预测分析。结果表明,果梅NAC基因家族包含106个NAC基因成员,根据系统发育特征将其分为12个亚族。PmNAC基因在果梅的8条染色体上呈现不均匀分布,多编码酸性氨基酸;大部分NAC蛋白为亲水蛋白,其二级结构多以无规则卷曲为主要构成元件,且三级结构相似;亚细胞定位预测显示,果梅NAC蛋白为典型的核蛋白。选取12个NAC基因家族成员,利用实时荧光定量PCR技术进行组织表达分析,结果表明,12个果梅NAC基因在不同组织中均有表达,但表达差异明显。其中3个基因(PmNAC54、PmNAC32、PmNAC68)在果皮中表达最高,4个基因(PmNAC60、PmNAC95、PmNAC51、PmNAC2)在果肉中表达最高,3个基因(PmNAC31、PmNAC62、PmNAC48)在嫩茎中表达最高,其余2个基因(PmNAC61和PmNAC71)分别在果胚和花芽中具有最高的表达,表达特征的差异表明它们可能具有不同的生物学功能。本研究结果为果梅NAC蛋白的进一步功能分析奠定了一定的理论基础。 相似文献
9.
番茄中有10个14-3-3蛋白成员,分别命名为Sl TFT1-Sl TFT10,这些蛋白成员虽具有一个共同的14-3-3蛋白结构域,但因它们所在的染色体、细胞亚环境的差异使得功能上呈现多样化,其中的Sl TFT3可能参与番茄的抗病应答,通过启动子分析预测可为后续的功能验证提供参考。生物信息学分析结果表明:Sl TFT3启动子中可能的转录起始位点是位于序列的2844-2894的T,Sl TFT3基因启动子上具有真菌诱导反应元件、热应激反应顺式作用元件、防御和应激反应中的顺式作用元件等,可能与抗逆反应相关。 相似文献
11.
低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响 总被引：2，自引：2，他引：0
【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。 相似文献
12.
Kyle Devey Michael Mucalo Gordon Rajendram Joseph Lane 《Communications in Soil Science and Plant Analysis》2015,46(4):72-80
Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing. 相似文献
13.
I.L. Boyd 《Biological conservation》2010,143(7):1664-1674
“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options. 相似文献
14.
Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage 总被引：1，自引：0，他引：1
Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate. 相似文献
15.
《中国生态农业学报》2014,(8)
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology, 相似文献
16.
M.A. Callaham Jr. J.M. BlairT.C. Todd D.J. KitchenM.R. Whiles 《Soil biology & biochemistry》2003,35(8):1079-1093
The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils. 相似文献
17.
Yoav Bashan Bernardo Salazar Ma. Esther Puente Macario Bacilio Robert Linderman 《Biology and Fertility of Soils》2009,45(6):585-594
To evaluate the feasibility of long-term desert reforestation technology of mixed vegetation, cardon cactus (Pachycereus pringlei) seedlings from indoor and outdoor nurseries were planted in the field adjacent to one seedling of potential legume nurse
trees: mesquite amargo (Prosopis articulata), yellow palo verde (Parkinsonia microphylla), and blue palo verde (Parkinsonia florida). Some of the planting holes were also supplemented with common dairy compost. Additionally, the combinations of legume tree–cactus
were inoculated with either a consortium of desert arbuscular mycorrhizal (AM) fungi, plant growth promoting bacteria (PGPB;
the diazotroph Azospirillum brasilense Cd, and the phosphate solubilizer Paenibacillus sp.), or a mixture of all. The field experiments were evaluated periodically during 30 months for survival and growth. Cardons
reared in an outdoor screen house survived better in the field than those reared in a controlled growth chamber and hardened
later outdoors. Association with any legume nurse tree increased survival and enhanced growth of untreated cardons. For cardons
growing alone, application of either compost, AM fungi, and all the treatments combined increased survival. For these plants,
no treatment affected plant growth during the first 3 months after transplanting. Later, all treatments, except for AM fungi,
enhanced plant growth. However, only 2 years after transplanting the enhanced growth effect of AM fungi was also significant.
In the presence of the legume nurse trees, transient positive effects on cardon growth were recorded. General evaluation after
30 months of cultivation showed that the treatments positively affected cardon growth when growing alone or in combination
only with mesquite amargo but not with the other two legume trees. This study proposes that young legume trees have the capacity
to enhance survival and growth of cardon cactus, depending on the legume cactus combination. Additional treatments such as
compost or PGPB can either amplify the effect or else attenuate it. 相似文献
18.
Grete Skrede Berit Karoline Martinsen Anne-Berit Wold Stein-Erik Birkeland Kjersti Aaby 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):193-208
Abstract A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species. 相似文献
19.
Detailed karyotypes and 4C DNA amounts have been studied in five cultivars of Cajanus cajan and 20 species belonging to Cajanus, Rhynchosia, Dunbaria, Flemingia and Paracalyx. C. cajan shows intraspecific variability and its karyotype is most similar to that of C. cajanifolius (sect. Cajanus) in the morphology and number of satellite chromosomes and the lack of any correlation between chromosome size within the complement and asymmetry. Karyotypes of C. lineatus and C. sericeus belonging to sect. Atylia are similar with respect to maximum r-index and the ratio of longest and shortest chromosomes in their respective complements. C. acutifolius (sect. Frutocosa) is distinct in having a very low ratio (1.44) between the longest and the shortest chromosomes, while in C. albicans, C. goensis, C. scarabaeoides (sect. Cantharospermum) there is no chromosome pair with r-index >2.0. C. mollis and C. volubilis (sect. Volubilis) show similarity with regard to ratio between the longest and the shortest chromosomes and a distinct chromosome pair with an arm ratio of 2.7 and C. platycarpus is distinct (sect. Rhynchosoides) in having the smallest ratio (1.36) between the longest and the shortest chromosomes. 4C DNA amounts in Cajanus and Rhynchosia vary between 3.28 pg to 11.69 pg and 4.92 pg to 11.13 pg respectively, while in Dunbaria, Flemingia and Paracalyx these vary between 4.70 and 7:06 pg. In the genus Cajanus karyotypic features and 4C DNA amounts agree with the sectional classification and bring out a close relationship between C. cajan and C. cajanifolius. This is supported by studies based on seed protein patterns, isozyme analysis, trypsin and chymotrypsin inhibitor patterns, RFLP and RAPD data and crossability relationships. A clear relation between DNA amount and abnormalities effecting the extent of pairing and recombination in interspecific hybrids shows the importance of this study in developing future breeding programme involving C. cajan and its wild relatives that are of potential value. 相似文献
20.
Several commercial fungicide seed treatments were evaluated for their possible effect on the survival of Bradyrhizobium japonicum on seeds and on the nodulation and yield of soybeans in a greenhouse and a field experiment. quinolate Pro (carbendazim and oxine copper), Vitavax 200FF (carboxin and thiram), and Monceren (pencycuron) had a small effect or no effect on the survival of B. japonicum and on the nodulation and yield of soybeans. They can thus be considered compatible with soybean seed inoculation. Germipro UFB (carbendazim and iprodione), Apron 35J (metalaxyl), and Tachigaren (hymexazol) decreased B. japonicum survival and the nodulation and yield of soybeans and thus cannot be considered compatible with soybean seed inoculation. 相似文献