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Bovine papilloma (BP) is a infectious papilloma induced by bovine papillomavirus (BPV).This disease is easily to recur and evolve into cancer,leading to severe loss of breeding industry.The author mainly summarize the gene types,genetic structure,function of genes and pathogenic mechanism of BPV,providing important references to further study the carcinogenic potential of BPV and to develop antiviral vaccines. 相似文献
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PANG Feng SHI Qiao-yun ZHU Hua-pei XU Kai-lian ZHAO Tian-jing LI Ya-ying PENG Dong-mei LI Guo-hua ZHOU Hai-long WANG Feng-yang 《中国畜牧兽医》2015,42(5):1088-1092
The PCR technology was used to amplify the E5 gene according to the genome sequence of bovine papillomavirus type 13 (BPV-13) Hainan strain with the GenBank accession number KM258443.2.Then it was ligated into pEGFP-N1 vector.The recombinant plasmid pEGFP-E5-N1 was transfected into HEK293 cells with liposomes by transient transfection.The expression of fusion protein was identified by fluorescence microscope, flow cytometry and Western blotting.The results showed that the E5 gene was 135 bp with the 100% homology with the sequence deposited in GenBank.Bright green fluorescence could be seen in HEK293 cells transfected by pEGFP-E5-N1.The expressed fusion protein was about 32 ku.This experiment laid the foundation for further study of the functions of E5 gene in eukaryotic cells. 相似文献
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基于海口市动物基因工程重点实验室获得的牛乳状瘤病毒13型(BPV-13)基因组序列信息(GenBank登录号:KM258443.2),以基因组为模板,扩增E5基因片段,将其连入pEGFP-N1质粒,构建重组质粒pEGFP-E5-N1,测序正确后瞬时转染HEK293细胞,应用荧光显微镜、流式细胞仪、Western blotting 鉴定融合蛋白的表达。结果显示,E5片段大小为135 bp;重组质粒pEGFP-E5-N1构建正确;荧光显微镜下观察转染重组质粒pEGFP-E5-N1的HEK293细胞,可见明亮的绿色荧光;流式细胞术分析结果显示,转染重组质粒pEGFP-E5-N1 48 h后,约55.59% 的细胞表达绿色荧光蛋白;Western blotting结果表明,表达的融合蛋白大小约为32 ku。本试验结果为下一步研究E5基因的作用机理奠定了基础。 相似文献
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Qingling Meng Chengcheng Ning Lixia Wang Yan Ren Jie Li Chencheng Xiao Yanfang Li Zhiyuan Li Zhihao He Xuepeng Cai Jun Qiao 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(4)
BackgroundBovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry.ObjectivesThe aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China.Methods122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree.ResultsTen genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions.ConclusionsGenotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows. 相似文献
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Hainisch EK Brandt S Shafti-Keramat S Van den Hoven R Kirnbauer R 《Equine veterinary journal》2012,44(1):107-111
Reasons for performing study: Infection with bovine papillomaviruses types 1 and 2 (BPV‐1, BPV‐2) can lead to the development of therapy‐resistant skin tumours termed sarcoids and possibly other skin diseases in equids. Although sarcoids seriously compromise the welfare of affected animals and cause considerable economic losses, no prophylactic vaccine is available to prevent this common disease. In several animal species and man, immunisation with papillomavirus‐like particles (VLP) has been shown to protect efficiently from papillomaviral infection. Hypothesis: BPV‐1 L1 VLPs may constitute a safe and highly immunogenic vaccine candidate for protection of horses against BPV‐1/‐2‐induced disease. Methods: Three groups of 4 horses each received 50, 100 or 150 µg of BPV‐1 L1 VLPs, respectively, on Days 0, 28 and 168. Three control horses received adjuvant only. Horses were monitored on a daily basis for one week after each immunisation and then in 2 week intervals. Sera were collected immediately before, 2 weeks after each vaccination and one and 2 years after the final boost and analysed by pseudovirion neutralisation assay. Results: None of the horses showed adverse reactions upon vaccination apart from mild and transient swelling in 2 individuals. Irrespective of the VLP dose, all VLP‐immunised horses had developed a BPV‐1‐neutralising antibody titre of ≥1600 plaque forming units (pfu)/ml 2 weeks after the third vaccination. Eight of 10 trial horses still available for follow‐up had neutralising antibody titres ≥1600 pfu/ml one year and ≥800 pfu/ml 2 years after the last immunisation. Conclusion: Intramuscular BPV‐1 L1 VLP vaccination in horses is safe and results in a long‐lasting antibody response against BPV‐1. Neutralisation titres were induced at levels that correlate with protection in experimental animals and man. Potential relevance: BPV‐1 L1 VLPs constitute a promising vaccine candidate for prevention of BPV‐1/‐2‐induced disease in equids. 相似文献
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Papillomaviruses are oncogenic DNA viruses and induce hyperplastic benign lesions of both cutaneous and mucosal tissues in their various hosts, including many domestic and wild animals as well as humans. There are some Papillomavirus genotypes that can infect hosts different from their own, such as BPV 1 and BPV 2 originated from cattle, which can also infect horses and are responsible for fibroblastic tumours in horses. This review article summarizes the origin and evolution of papillomaviruses as an etiological agent in the historical process. The main focus in this review is the evaluation of the interactions between high‐risk papillomavirus oncoproteins and programmed cell‐death pathways. It further exemplifies the role of these interactions in the malignant cell transformation process. In parallel with this, the use and importance of the bovine model system to enlighten the papillomavirus‐associated cancers is discussed with an in‐depth examination. Furthermore, it focuses on the epidemiological situation of BPV infections in Turkey in the cattle herds. 相似文献
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Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells 
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下载免费PDF全文 N. Terron‐Canedo W. Weir L. Nicolson C. Britton L. Nasir 《Veterinary and comparative oncology》2017,15(3):764-774
Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses. MicroRNAs (miRNAs), small non‐coding RNAs that regulate essential biological and cellular processes, have been found dysregulated in a wide range of tumours. The aim of this study was to identify miRNAs associated with ES. Differential expression of miRNAs was assessed in control equine fibroblasts (EqPalFs) and EqPalFs transformed with the BPV‐1 genome (S6‐2 cells). Using a commercially available miRNA microarray, 492 mature miRNAs were interrogated. In total, 206 mature miRNAs were differentially expressed in EqPalFs compared with S6‐2 cells. Aberrant expression of these miRNAs in S6‐2 cells can be attributed to the presence of BPV‐1 genomes. Furthermore, we confirm the presence of 124 miRNAs previously computationally predicted in the horse. Our data supports the involvement of miRNAs in the pathogenesis of ES. 相似文献
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Equine sarcoid of the glans penis with bovine papillomavirus type 1 in a
miniature horse (Falabella)
Kikumi OGIHARA Akikazu ISHIHARA Makoto NAGAI Kazutaka YAMADA Testuya MIZUTANI Mei HARAFUJI Hisanari NISHIO Hiroo MADARAME 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):1016
A 23-year-old Falabella gelding kept in Tochigi, Japan, for more than 20 years presented with a recurrent mass of the glans penis that was first noticed about a year earlier. Partial phallectomy was performed with no adjunctive therapy for local regrowth of the mass. The horse was euthanized 3 months after surgery for urinary retention due to suspected regrowth. The resected mass affected the genital and urethral mucosa of the glans penis, and was diagnosed as equine sarcoid by histopathology and identification of bovine papillomavirus (BPV) DNA. Phylogenetic analysis of the BPV genome of the sarcoid showed high sequence homology to BPV type 1 (BPV-1) from Hokkaido, Japan, suggesting a geographical relationship for BPV-1 in Japan. 相似文献
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The bovine enterovirus type 1 (BEV-1) infection has a wide range of host spectrum including humans. In this study, seroprevalence of BEV-1 was investigated in eight mammalian species. Blood serum samples were collected from 244 humans, 1520 cattle, 272 horse, 126 dog, 281 sheep, 477 goat, 18 camel (Camelus dromedarius) and 82 gazelle (Gazella subgutturosa subgutturosa) in different regions of Turkey. Microneutralization tests showed that gazelle and camel did not have any seropositivities, but seropositivities were detected in humans (30.3%), cattle (64.8%), horse (12.8%), dog (3.2%), sheep (32.8%) and goat (27.6%). 相似文献
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为了解牛乳头瘤病毒1型（bovine papillomavirus genotype 1,BPV-1）广西GX01株全基因组序列、结构特征及遗传变异情况,同时了解该毒株引起宿主产生的病理组织学变化情况,本研究选取广西贺州市患病牛皮肤肿瘤样物制作石蜡切片后镜检观察,提取病料DNA,以乳头瘤病毒L1基因的简并引物FAP59/FAP64进行PCR扩增以确定此病毒的基因型,根据GenBank中BPV参考株设计嵌套引物,对GX01株进行全基因组扩增、克隆测序及序列分析。病理组织学检查结果显示,可在病变部位发现表皮细胞增生、肿胀,角质过度及挖空细胞等乳头瘤病毒感染的特征性病变。序列分析结果表明,GX01株为BPV-1,其全基因组长为7 945 bp,包含E1、E2、E4、E5、E6、E7、L1、L2 8个开放阅读框,符合BPV-1型基因组的结构特征;GX01与BPV-1参考株全基因组核苷酸序列同源性为98.6%~99.6%,与BPV-2型参考株（M20219.1）、BPV-13型参考株（JQ798171.1）同源性分别为86.9%和87.2%。GX01株为广西地区首次经检测确认并测定全基因组序列的牛乳头瘤病毒。本研究为广西地区乃至全国的牛乳头状瘤的病原鉴定、流行规律、遗传变异、疫源追溯及科学防控提供了基础数据。 相似文献
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Brandt S Schoster A Tober R Kainzbauer C Burgstaller JP Haralambus R Steinborn R Hinterhofer C Stanek C 《Equine veterinary journal》2011,43(2):202-209
Reasons for performing the study: Equine hoof canker is a chronic proliferative pododermatitis of as yet unknown aetiology. Like equine sarcoid disease, canker is a therapy‐resistant disorder characterised by hyperkeratosis, acanthosis and a marked tendency to recur. Hypothesis: There is an association of sarcoid‐inducing bovine papillomaviruses of types 1 and 2 (BPV‐1, BPV‐2) with hoof canker disease. Methods: Using PCR‐based techniques, we assessed canker tissue, intact skin and/or peripheral blood mononuclear cells (PBMCs) of 25 canker‐affected horses for the presence of sarcoid‐associated BPV‐1 and ‐2. Results: Conventional PCR revealed BPV‐1/‐2 DNA in 24/24 canker, 12/13 skin and 10/11 PBMC DNA isolates. Using inverse PCR, full‐length BPV episomes were detected in 1/5 canker specimens. Sequencing of viral early and late genes amplified from canker, intact skin and PBMC DNA of 2 cases revealed an overall identity of 98% to BPV‐1. Viral DNA loads amounted to ≤16 copies per cell in canker tissue and intact skin, and to ≤0.35 copies per PBMC, as determined by quantitative PCR. Using RT‐PCR, the viral major oncogene E5 was shown to be transcribed in 2/4 canker tissue specimens and 5/7 PBMC isolates. Immunocapture PCR from 7 canker and 6 skin extract supernatants revealed capsomere‐associated viral DNA in one canker and one skin sample. Hoof tissue, skin and PBMCs collected from 13 individuals with no signs of canker or BPV‐related malignancies scored negative throughout the experiments. Conclusion: These findings suggest that the observed presence of BPV‐1/‐2 in canker‐affected horses is not coincidental but indicative of an active contribution to hoof canker disease. Potential relevance: The use of antivirals and/or immune modulators may help improving canker therapy. 相似文献
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R. HARALAMBUS J. BURGSTALLER J. KLUKOWSKA‐RÖTZLER R. STEINBORN S. BUCHINGER V. GERBER S. BRANDT 《Equine veterinary journal》2010,42(4):327-331
Reasons for performing study: Sarcoids are nonmetastasising, yet locally aggressive skin tumours that constitute the most frequent neoplasm in equids. Infection by bovine papillomaviruses types 1 and 2 (BPV‐1, BPV‐2) has been recognised as major causative factor in sarcoid pathogenesis, but a possible correlation of intralesional virus load with disease severity has not been established thus far. Hypothesis: Given the pathogenic role of BPV‐1 and BPV‐2 in sarcoid disease, we suggest that intralesional viral DNA concentration may reflect the degree of affection. Methods: Severity of disease was addressed by recording the tumour growth kinetics, lesion number and tumour type for 37 sarcoid‐bearing horses and one donkey. Viral load was estimated via quantitative real‐time PCR (qPCR) of the E2, E5, L1 and L2 genes from the BPV‐1/‐2 genome for one randomly selected lesion per horse and correlated with disease severity. Results: Quantitative PCR against E2 identified viral DNA concentrations ranging from 0–556 copies/tumour cell. Of 16 horses affected by quiescent, slowly growing single tumours or multiple mild‐type lesions, 15 showed a viral load up to 1.4 copies per cell. In stark contrast, all equids (22/22) bearing rapidly growing and/or multiple aggressive sarcoids had a viral load between 3 and 569 copies per cell. Consistent results were obtained with qPCR against E5, L1 and L2. Conclusions: While tumours of the same clinical type carried variable virus load, confirming that viral titre does not determine clinical appearance, we identified a highly significant correlation between intralesional viral load and disease severity. Potential relevance: The rapid determination of BPV viral load will give a reliable marker for disease severity and may also be considered when establishing a therapeutic strategy. 相似文献
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吉林省黄牛“猝死症”的病因学研究 总被引：2，自引：0，他引：2
１９９４年以来，对吉林省黄牛“猝死症”的病因进行了多方面的研究，包括细菌学检验、病毒学检验、人工感染试验、毒物检验、牛毛硒元素分析及病尸病理形态学观察。结果表明，Ａ型魏氏梭菌是主要病原菌，其他细菌有协同作用；牛冠状病毒或粘膜病病毒感染、缺硒等为本病的诱因 相似文献
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Experimental and field studies have proven that the bovine herpesvirus type‐4 (BHV‐4) infection leads to various reproductive system problems. In this study, the role of BHV‐4 infection in repeat breeding was investigated serologically. Eighty‐four samples were obtained from repeat‐breeding diagnosed cows in two organized dairy herds; an equal number of healthy cows were sampled from the same farms. The rest of the samples (105) were obtained from reproductively normal cows that were breeding in 18 small enterprises as a control group. The seropositivity proportion in repeat‐breeding diagnosed cows was found to be significantly higher (69% (58/84)) than other cows (44% (37/84)) on the same farms. The lowest antibody positivity value for BHV‐4 was detected as 24.7% (26/105) in the samples from family‐type small farms. The odds ratio (OR) value was calculated as 2.834 in repeat‐breeding diagnosed and healthy cows on the same farms, while 6.778 was determined in cows with and without reproductive problems on organized farms compared to small farms. As a result, the BHV‐4 infection can be considered one of the reasons for repeat breeding besides other reproductive disorders. 相似文献