共查询到19条相似文献，搜索用时 328 毫秒
1.
为了掌握山东地区水貂出血性肺炎流行情况,从2012年山东省内多个养貂社区进行水貂出血性肺炎流行病学调查。从病貂中分离获得48株绿脓杆菌,分别测定了分离株的生化特性、血清型以及部分菌株对小鼠与水貂的半数致死量（LD50）,同时运用Eric-PCR方法对分离进行了进行分型。结果显示,48株分离菌株与标准菌株生化特性基本一致,血清型G型为36株,血清型Ⅰ型为7株,血清型C型为4株;48株分离株大多数对恩诺沙星最敏感;动物试验显示,48株分离株对小鼠与水貂均能致病,所选4株分离株对小鼠与水貂的LD50有差异,且水貂对所选菌株显著敏感于小鼠;分子分型分为13个基因型。结果表明,该地区水貂出血性肺炎病原呈现遗传多样性。本试验为水貂出血性肺炎的防治提供了理论依据。 相似文献
2.
2013～2016年山东省、河北省、黑龙江省、吉林省的部分水貂养殖场发生了严重的水貂出血性肺炎,导致水貂大量死亡,为了客观评估水貂出血性肺炎的危害及为综合防控提供参考依据,试验对423个水貂病例样本进行分离鉴定并且对所分离的细菌进行了形态特征观察[1]、16SrRNA基因测序、血清型鉴定、小鼠毒力试验测定、生化试验及药敏试验测定.结果表明:423个样本中分离到90株绿脓杆菌,其中最为流行的血清型为G型(25株)、B型(21株)、I型(17株)、C、E型及未定型(27株);并筛选出12株菌株对小鼠有很强的毒力性.生化试验结果表明:同一血清型的不同菌株之间也存在明显差异.药敏试验结果表明:12株菌株对强力霉素、林可霉素均耐药;对阿米卡星为敏感;对庆大霉素等4种抗生素均为耐药或介于中间;对环丙沙星等6种抗生素的敏感性不同;对氧氟沙星为敏感或介于中间. 相似文献
3.
2013~2016年山东省、河北省、黑龙江省、吉林省的部分水貂养殖场发生了严重的出血性肺炎,导致水貂大量死亡,为了客观评估水貂出血性肺炎的危害及为综合防控提供参考依据,试验对423个水貂病例样本进行分离鉴定并且对所分离的细菌进行了形态特征观察、16SrRNA基因测序、血清型鉴定、小鼠毒力试验测定、生化试验鉴定及药敏试验测定。结果表明:423个样本中分离到68株肺炎克雷伯氏菌,其中最为流行的血清型为K2型(35株);并筛选出4株菌株对小鼠有很强毒力性;生化试验结果表明同一血清型的不同菌株之间也存在明显差异;药敏试验结果表明,4株K2型肺炎克雷伯氏菌强毒菌株对氧氟沙星、阿米卡星2种抗生素均为敏感或介于中间;对强力霉素、环丙沙星等8种抗生素均耐药;对诺氟沙星、庆大霉素等4种抗生素均为耐药或介于中间。 相似文献
4.
为确诊引起水貂死亡的主要细菌性病原,对采集的病料进行细菌分离和培养,分离出3株菌。通过对分离株的形态、生化和培养特性研究,分子生物学和血清型鉴定,并对其中一分离菌株生长曲线、毒力和免疫原性进行测定。结果表明,分离株均为血清C型水貂源绿脓杆菌;分离株16S r RNA基因序列与已知的绿脓杆菌相应序列同源性为99.7%~99.98%不等;生长曲线结果表明,C型绿脓杆菌4~14 h为对数生长期;对小鼠的半数致死量(LD_(50))为8.11×105 CFU;制备的单价疫苗对小鼠的免疫原性良好,攻毒保护率为100%,对照组小鼠全部死亡。 相似文献
5.
2011~2013年间,从山东省、河北省和江苏省等地的多个貂场疑似水貂出血性肺炎病貂的肺脏、肝脏和脾脏中分离到425株疑似绿脓杆菌。用绿脓杆菌的OprF和PEA基因的PCR引物对分离菌株的目的基因扩增,经核苷酸测序确定其均为绿脓杆菌序列。通过日本生研株式会社血清学分型系统进行分型：G型376株,占88.5%;B型34株,占8%;C型12株,占2.8%;E型3株,占0.7%。取其中6株细菌（G血清型3株,其他血清型各1株）进行详细生化试验,结果显示该6株菌均符合绿脓杆菌的生化特性,并且从这6株中选4株细菌（每血清型各1株）腹腔接种小白鼠和气管内接种水貂,结果表明4株菌对其均有致病性。 相似文献
6.
7.
《中国兽医杂志》2016,(9)
为了探明水貂出血性肺炎病原和流行特点,2011-2012年对山东省15个水貂养殖场进行水貂出血性肺炎流行病学调查。从病貂中分离出19株绿脓杆菌,分别测定了分离株的血清型,同时应用肠杆菌科基因间重复序列聚合酶链反应(ERIC-PCR)对菌株进行DNA分型。结果 19株分离株中血清型G型15株,血清型B型2株,血清型E型和I型各1株;ERIC-PCR谱型表现为7种基因型,其中15株G型株存在于谱型Ⅰ~Ⅶ中(Ⅵ除外),B型株属于谱型Ⅱ,I型株属于谱型Ⅳ,E型株属于谱型Ⅵ。表明山东地区水貂出血性肺炎由绿脓杆菌引起,其分离株血清型以G型为主,基因型呈现多样性。 相似文献
8.
为了获得针对水貂出血性肺炎的特异性疫苗,试验从分离自2012年水貂出血性肺炎病例的24株绿脓杆菌中筛选出血清G型(D4株)与血清C型(C1株)各1株;同时研制血清G型、血清C型及血清G-C型二价灭活疫苗,分别免疫小鼠与水貂,攻病原菌,测定疫苗的保护率、血清抗体消长规律。结果表明:分离株D4与C1在30代内菌体含量稳定,D4株在24代内毒力稳定,C1株在21代内毒力稳定;血清G型与C型单价疫苗都可对50倍LD50本型病原菌产生完全保护,二价灭活疫苗可对50倍LD50血清G型与C型病原菌完全保护。一免后第21天小鼠血清抗体水平达到峰值,抗G型与C型菌中和抗体效价为1∶3 200,1∶6 400,在35 d后抗体水平下降;二免后,水貂55 d内血清中和抗体维持在较高水平。说明D4株与C1株可以作为良好的水貂出血性肺炎疫苗的候选株,制备的二价灭活疫苗能对水貂产生良好保护力,为水貂出血性肺炎防治奠定坚实基础。 相似文献
9.
为了鉴定引起水貂发生出血性肺炎的病原体,试验采用病原分离鉴定、分离株16S rRNA扩增与序列分析、分离株对小鼠致病力试验、分离株血清分型鉴定、大肠杆菌H基因型鉴定、分离株药敏试验和分离株动物感染试验对来自山东省、辽宁省、河北省和黑龙江省的28份具有典型出血性肺炎症状死亡水貂的病料进行鉴定。结果表明:混合感染12例,单一病原菌感染16例,镜检可见短小的阴性杆菌和两端钝圆的阴性长杆菌; 16S rRNA扩增与序列分析共分离出22株绿脓杆菌和18株大肠杆菌,其中有4株对小鼠致死率达到50%以上,9株对小鼠致死率在10%～40%;从22株绿脓杆菌中共分离出8株G型、7株E型和7株D型,未检测出大肠杆菌血清型,大肠杆菌H基因型为H11;绿脓杆菌分离株对环丙沙星、妥布霉素、阿米卡星等6种药物敏感,大肠杆菌分离株对环丙沙星、妥布霉素、阿米卡星等10种药物均耐药;大肠杆菌和绿脓杆菌分离株均可导致水貂死亡并造成肺泡壁毛细血管瘀血、脾脏淋巴细胞数量减少、肺脏上皮细胞坏死、红髓内巨噬细胞数量增多等明显病理变化。说明目前引起水貂出血性肺炎的病原体已由传统的绿脓杆菌单一感染逐渐转变为大肠杆菌和绿脓杆菌的混合感染。 相似文献
10.
《中国兽医学报》2020,(4)
为分析河北部分地区狐狸源肺炎克雷伯菌(Klebsiella pneumoniae)的荚膜血清型、毒力基因流行情况及致病性,从河北地区养殖场中采集患病狐狸肺脏、心血、肝脏等病料组织217份,分离得到63株致病性肺炎克雷伯菌。采用PCR法分别检测63株肺炎克雷伯菌荚膜血清型及19种毒力基因;通过人工感染小鼠致病试验验证4株优势血清型流行株的半数致死量(LD_(50))。结果显示,63株肺炎克雷伯菌中23株为K2血清型,7株为K20血清型。63株肺炎克雷伯菌携带13种毒力基因,毒力基因fimH、mrkD、wabG、uge、ybt、ybtA、alls、ompK35等检出率均在58.73%以上,其他毒力基因检测率较低;分离菌株呈现多重毒力基因型,同时携带9种毒力基因的菌株最多,占致病性菌株的33.33%。优势血清型流行株KD1(K20)和KD45(K2)的致病性最强,其LD_(50)分别为3.16×10~5,3.16×10~6 CFU/mL。本研究为该地区肺炎克雷伯菌病的防控提供参考。 相似文献
11.
C O Thoen E M Himes W D Richards J L Jarnagin R Harrington 《American journal of veterinary research》1979,40(1):118-120
Mycobacteria were isolated from 14.3% of the tissues submitted to the National Veterinary Services Laboratories over a 5-year period (July 1, 1972, to June 30, 1977). The isolates were identified by drug susceptibility, and biochemical and serologic tests. Mycobacterium bovis isolated from tissues of cattle originating in 32 states and Puerto Rico accounted for 78% of the acid-fast isolations. Of the Mycobacterium bovis isolates, 4% were from tissues in which no microscopic tuberculous granulomas were observed on examination of tissue sections. Of the 119 Mycobacterium avium isolates, 18 were serotype 1, 44 were serotype 2, and 45 isolates represented 12 other serotypes. 相似文献
12.
珠三角及邻地鸭疫里默氏杆菌主要生物学特性的研究 总被引：1，自引：0，他引：1
为明确珠三角地区鸭疫里默氏杆菌(RA)的药物敏感性、血清型类型及其同源性,本研究对100株鸭源及鹅源RA进行分离与鉴定,并采用琼脂扩散试验进行血清型分类及其对12种药物的敏感性试验;选取10个代表株测定其对7日龄雏鸭的致病性及ompA基因序列,比较相互间的同源性以及其它生物特性的关系。结果表明:分离株的生化特性基本一致,代表株对雏鸭具有强的致病性;所有菌株具有一重以上的耐药性,63株细菌具有双重或多重耐药性,97%的菌株对奥格门丁敏感,其它药物敏感度依次为壮观霉素、青霉素、环丙沙星、氨苄西林、链霉素、诺氟沙星、罗红霉素、磺胺甲噁唑、利福平和卡那霉素;93%的菌株对庆大霉素耐药,表明该地区RA对大部分常用药物产生一重或多重耐药;100株菌株分属于4种血清型,其中83株属于血清1型,属于其它3个血清型的菌株分别有10株、5株和2株,以第4种血清型致病性最强;10个代表株中9株的外膜蛋白基因序列同源性达96%以上,5株为100%,1株与其它株的同源性为90.1%～90.4%,表明10株菌的外膜蛋白基因序列的同源性较高,外膜蛋白基因与血清型和菌株的致病性无直接关系。 相似文献
13.
L Fodor J Varga I Hajtós T Molnár 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(4):241-247
The biochemical and serological characteristics of 486 P. haemolytica and 31 P. trehalosi strains (517 in total) isolated from different lesions of cattle, sheep, goats, pigs and poultry were examined. A total of 476 P. haemolytica strains (97.9%) showed the characteristics typical of the former biotype A of P. haemolytica, while 10 isolates (2.1%), all from poultry, could not be biotyped. A total of 481 strains (93.0%) could be assigned to one of the 17 serotypes of P. haemolytica-P. trehalosi and 36 strains (7.0%) could not. The majority (83.6%) of the cattle isolates were serotypes A1 and A2. Among strains isolated from sheep all serotypes of P. haemolytica could be identified with the exception of A14, but serotypes A1, A2, A6, A8 and A5 were the most frequent. The overwhelming majority (94%) of the caprine isolates were A2, other serotypes occurred only sporadically. The pig isolates, which could be isolated only very rarely, represented different serotypes, while none of the 10 strains isolated from poultry could be biotyped or serotyped. 相似文献
14.
15.
为了解陕西省宁强县部分地区引起仔猪黄白痢的致病性大肠埃希菌的主要血清型及其主要毒力因子,用细菌分离培养方法,从宁强县5个猪场采集的60份有疑似黄白痢症状仔猪的肛门拭子中分离出20个大肠埃希菌菌株。经菌落形态观察、革兰染色镜检、培养特性、生化试验、血清型鉴定,确定分离菌株均为致病性大肠埃希菌。血清分型表明,20个分离菌株中有18株能确定血清型,分布在7个血清型中,其中优势血清型为O8(8株),占总数的40%。药物敏感性试验表明,分离菌株对临床常用药物具有抗药性。毒力因子的PCR检测结果表明,分离菌株均不携带STa、STb、LT和SLT-2e四个毒力基因。 相似文献
16.
Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine 总被引：14，自引：0，他引：14
One hundred and forty-one isolates of Haemophilus pleuropneumoniae from Iowa and Illinois swine were characterized morphologically and biochemically and serotyped by rapid slide agglutination (RSA) and indirect fluorescent antibody (IFA) tests. Hyperimmune antisera were produced in rabbits using inactivated whole-cell suspensions of the reference strains for H pleuropneumoniae serotypes 1 to 7 and strain 202, representing the taxon "minor group." Cross testing of the reference strains and reference antisera indicated the antisera to be essentially serotype-specific, although reactivity of some antisera with heterologous strains was observed. Cultures of the 141 isolates formed adherent or smooth colonies or mixtures of these colony forms. Adherent and smooth colony types were found in all serotypes identified. Microscopic and biochemical characteristics of all isolates were typical of those previously described for H pleuropneumoniae. The overall incidence of H pleuropneumoniae serotypes was serotype 5, 55.3%; serotype 1, 34.0%; serotype 7, 7.8%; and nontypeable, 2.8%. Comparing the 2 test procedures, 87.2% of the isolates could be typed by RSA, and 66.0% could be typed by IFA. Cross-reactions between serotype 4 antisera and serotype 5 and 7 isolates were common with the IFA test. The reactions with serotype 7, but not serotype 5, were eliminated by cross adsorption of serotype 4 antisera. There was good correlation between the 2 test procedures, but RSA was judged to be more specific and sensitive than IFA. 相似文献
17.
Cho WS Chae C 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(2):90-94
Twelve Actinobacillus pleuropneumoniae serotypes were differentiated by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified fragments from the outer membrane lipoprotein (omlA) gene. All 12 reference serotypes and 80 field isolates produced the expected 950-base pair (bp) fragment of the omlA gene by PCR. Combining the RFLP patterns obtained with SfaNI, Bst71I, AluI, NciI, nine distinct patterns were observed in the 12 serotype reference strains. The PCR-based RFLP analysis of omlA genes allows differentiation among the 12 serotypes, with the exception of group 1 (serotypes 1, 9 and 11), and group 2 (serotypes 2 and 8). When the PCR products from the 70 field isolates were subjected to RFLP analysis, 68 showed the same RFLP patterns as their respective serotype reference strain. Two isolates that could not be typed had the same RFLP patterns as those of serotype 5. These results suggest that PCR-based RFLP analysis of the omlA genes may be of value in differentiating among 12 A. pleuropneumoniae serotypes. 相似文献
18.
An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes--evaluation of isolates from lungs and tonsils of pigs 总被引：14，自引：0，他引：14
The genetic variability of a gene coding for an outer membrane lipoprotein (omlA) was used to develop a PCR typing system for Actinobacillus pleuropneumoniae. Sequence differences in the middle region of the gene divided the A. pleuropneumoniae serotypes in five distinct groups. Group I included serotypes 1, 9, 11 and 12 (omlA l), Group II consisted of serotypes 2 and 8 (omlA II), Group III included serotypes 3, 6 and 7 (omlA III), Group IV (omlA IV) consisted of serotype 4 and Group V of serotypes 5a, 5b and 10 (omlA V). The sequence differences were utilized to construct PCR primers specific for each group, except of Group IV, as the amplicon of serotype 4 could be separated from Group III by size. Together with a PCR apx typing system, the omlA PCR typing system could discriminate the majority of A. pleuropneumoniae serotypes of biovar 1 except of serotypes 1, 9 and 11 and serotypes 2 and 8. The PCR typing system was tested on 102 field strains of A. pleuropneumoniae isolated from lungs of diseased pigs. The serotyping results of the investigated field strains were in agreement with the apx and omlA gene patterns found in the reference strains of the bacteria, with the exception of the omlA gene of five strains of serotype 8. To examine the apx and omlA gene pattern of tonsil isolates, the PCR typing system was tested on a total of 280 A. pleuropneumoniae field strains isolated from tonsils of pigs. Agreement between serotyping and DNA typing was found in 96% of the isolates using the apx gene patterns and in 89% of the isolates using the omlA gene. The same serotype specific apx/omlA gene pattern was thus found in the majority of the tonsil isolates and in isolates from diseased lungs. Most of the differences in the omlA gene were found in 18 tonsil isolates of serotype 12. The omlA/apx PCR typing system described in the present study makes it possible to determine the type specificity of the majority of A. pleuropneumoniae isolates by simple PCR technique and enables phenotype independent characterization of isolates non-typable by serotyping. 相似文献
19.
SHEN Xue-huai ZHANG Dan-jun PAN Xiao-cheng ZHAO Rui-hong DAI Yin HU Xiao-miao 《中国畜牧兽医》2017,44(12):3663-3669
In order to investigate the situation of predominant strain and antibiotic resistance of avian Salmonella in Anhui province, 42 strains Salmonella were isolated and identified from 116 suspected samples by culture characteristics, microscopic examination, biochemical test, PCR and serological identification,then drug resistance was analyzed by Kindy-Bauer method. There were 5 groups of 11 serotypes in 42 strains Salmonella, of which 30 strains belonged to B serogroup, 2 strains to A serogroup, 7 strains to D serogroup, 2 strains to C1 serogroup, 1 strain to C2 serogroup, and Salmonella Typhimurium of B serogroup were the predominant serotypes. All Salmonella isolates resistant rates to amikacin, levofloxacin and ciprofloxacin were less than 20%, and the resistance rates to ampicillin, streptomycin, furazolidone, spectinomycin, cotrimoxazole and doxycycline were more than 50%, while resistance rate to ampicillin was 97.62%, and 41 isolates had multiple drug resistance. The results showed that Salmonella Typhimurium was the predominant serotypes of avian Salmonella in Anhui, and isolates showed multiple drug resistance. This study would provide the data basis for the comprehensive prevention and treatment of clinical medicine of avian Salmonella. 相似文献