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1.
试验旨在探索致脑膜炎粪肠球菌对体外血脑屏障功能的影响，寻求稳定的构建体外血脑屏障损伤模型的方法。选用1周龄ICR小鼠进行原代脑微血管内皮细胞（BMEC）分离并通过BMEC特有的凝血因子Ⅷ（coagulation factor Ⅷ，Factor Ⅷ）进行荧光标记，鉴定分离的细胞并判定分离率。选用1~3日龄ICR小鼠分离星形胶质细胞并通过其特有的胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）对进行荧光标记，鉴定分离的细胞并判定分离率。将原代细胞传至3代，用Transwell二维小孔分别构建单层BMEC和BMEC与星形胶质细胞共培养的血脑屏障模型。选用致脑膜炎粪肠球菌、非致脑膜炎粪肠球菌和大肠杆菌DH5α感受态细胞分别与构建的体外血脑屏障模型共培养，评估致脑膜炎粪肠球菌对体外血脑屏障模型的穿透性。通过4 h渗透试验、荧光素钠通透性试验和血脑屏障损伤相关标志基因基质金属蛋白酶2（MMP-2）表达量的检测，评估致脑膜炎粪肠球菌对体外血脑屏障模型的影响。结果显示，试验成功分离到BMEC和星形胶质细胞，纯度分别达90%和95%以上。细菌穿透试验结果显示，所选的3株细菌只有致脑膜炎粪肠球菌可穿越体外血脑屏障模型。4 h渗透试验和荧光素钠通透性试验结果显示，共培养模型优于单层BMEC模型。与其他组相比，构建的体外血脑屏障与致脑膜炎粪肠球菌共培养组荧光素钠的穿透力更高，MMP-2基因的表达量明显上升。综上，BMEC与星形胶质细胞共培养的模型优于单层BMEC模型，致脑膜炎粪肠球菌可穿越体外血脑屏障模型，并介导体外血脑屏障损伤，本试验结果为揭示致脑膜炎粪肠球菌对体外血脑屏障损伤机制提供了理论依据。 相似文献
2.
《中国兽医杂志》2015,(12)
探讨获取纯度较高的原代猪脑微血管内皮细胞的分离和培养方法。采用1月龄的三元杂交猪,通过两次酶消化、BSA和Percoll非连续梯度离心获得较纯的脑微血管段后,接种于涂布有鼠尾胶的培养瓶进行原代培养;相差显微镜观察细胞并进行纯化和传代,采用Ⅷ因子相关抗原免疫荧光检测法对培养的细胞进行鉴定。结果表明,培养24 h即可见细胞从贴壁的脑微血管段周围爬出,细胞呈短梭形,集落呈典型的"鹅卵石样",区域性单层生长,6~7 d内皮细胞开始融合,血管内皮细胞特异性标志物Ⅷ因子相关抗原表达阳性。说明本试验成功的培养出了纯度较高的脑微血管内皮细胞,为后续的体外血脑屏障模型的建立奠定了基础。 相似文献
3.
《中国兽医学报》2016,(7):1151-1156
为了研究兔脑炎原虫如何破坏血脑屏障,侵入脑组织并引起脑肉芽肿形成,本试验采用IG染色、MGP染色,TUNEL染色和anti-GFAP染色等方法对26只有典型脑炎原虫病神经症状的病兔和10只健康兔的脑组织进行了详细研究。结果显示:病兔大脑皮质有少部分锥体细胞、血管内皮细胞及星形胶质细胞发生不同程度的凋亡。病损的血管旁有时检出兔脑炎原虫集落,附近的脑组织有巨噬细胞和小胶质细胞聚集形成的细胞性肉芽肿。用特殊染色可在血管内皮细胞、单核细胞和巨噬细胞中检出大量脑炎原虫和集虫泡。大量星形胶质细胞在病损的脑组织部位增生,包绕在聚集的巨噬细胞和小胶质细胞周围形成了肉芽肿。其粗大的足突在血管周围形成了厚层血脑屏障。总之,兔脑炎原虫通过导致血管内皮细胞和星形胶质细胞凋亡的方式而破坏了血脑屏障;通过侵入血管内皮细胞和单核细胞的方式进入脑组织,引起脑损伤。 相似文献
4.
血脑屏障（Blood brain barrier，BBB）是指脑壁与神经胶质细胞形成的阻止血液中的物质进入大脑的一道屏障，是维持脑内环境稳定所必须的膜性结构。因其位置的特殊性具备内皮细胞之间覆盖重叠，紧密连接，有效地阻止大分子物质通过连接处；连续不断的一层基底膜紧紧包围着内皮细胞；神经胶质细胞的终足包围住脑毛细血管壁的外表面的结构特征。从而形成了BBB的多层膜性结构，构成了脑组织的防护性屏障[1]。现对动物血脑屏障的紧密连接结构和细胞结构及其在神经性疾病中的应用，进行综述。 相似文献
5.
大鼠脑皮质微血管内皮细胞的分离培养及鉴定 总被引：1，自引：0，他引：1
本研究目的在于建立一种简易可行、获得细胞纯度较高的体外分离、培养大鼠脑皮质微血管内皮细胞的方法,为进一步研究其功能打好基础。新生SD大鼠,分离大脑皮质,经匀浆、两步过滤法获得大鼠脑微血管段,用胶原酶消化后,在37℃、5%CO2培养箱中进行原代细胞培养。通过差速消化和差速贴壁方法并结合倒置显微镜下形态学的观察进行传代和纯化,利用第Ⅷ因子相关抗原免疫荧光检测法对培养的细胞进行鉴定。结果显示:成功分离培养了大鼠脑微血管内皮细胞,倒置显微镜下单个细胞呈多角形,互相融合后呈"铺路石"样,单层贴壁生长;免疫荧光检测第Ⅷ因子相关抗原呈阳性。说明本研究建立的大鼠脑微血管内皮细胞的体外培养方法,程序简单,获得的细胞纯度较高。 相似文献
6.
应用组织学方法对猪延髓微血管及静脉注射呕吐毒素(DON)后在猪延髓的分布进行了初步观察.结果显示,进入延髓的动脉有较厚的弹性纤维膜,平滑肌层厚度为弹性膜的一半,向背侧延伸的分支,管壁薄,仅见内皮细胞和周细胞.在网状结构与纤维束间见有微血管,管壁未见弹性纤维.电镜免疫组化染色结果显示,延髓最后区毛细血管内皮细胞间有的部位有紧密连接,而有些部位缺失,内皮细胞与胶质细胞之间有较宽的空隙,有些部位缺少周细胞.D()N免疫阳性反应为棕褐色或棕黄色颗粒,主要分布于延髓微动脉管壁及最后区毛细血管周边神经元细胞质中,细胞核内量少或无反应.结果表明,猪延髓内不同部位的微血管的分布及结构均有差异,为猪的中枢神经系统脑血管的组织学增加了内容. 相似文献
7.
《中国兽医学报》2016,(6):979-984
为了研究兔脑炎原虫(EC)性脑肉芽肿的病理学发生及形态特点,通过特殊染色、TUNEL染色、anti-GFAP染色和anti-NGF染色对26只病兔和10只健康对照兔的脑组织进行了详细地观察与分析。结果显示,EC能侵入血管内皮细胞引起血管损伤,可被单核细胞吞噬但不被消灭,并随之进入脑组织,导致大量星状胶质细胞增生,包围脑炎原虫集落或带虫的巨噬细胞,从而形成肉芽肿。EC性肉芽肿主要有3种形态,即细胞性肉芽肿、增生性肉芽肿和坏死性肉芽肿。构成脑肉芽肿的主要细胞为上皮样细胞、小胶质细胞、星状胶质细胞及内皮细胞,而淋巴细胞较少。结论,EC可侵入血管内皮破坏血脑屏障或随单核细胞的浸润而进入脑组织;脑肉芽肿的形成限制了EC的扩散;3种形态的脑肉芽肿实际上是一个病理过程的不同发展阶段。 相似文献
8.
9.
试验旨在研究过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptors γ,PPARγ）对猪血管内皮细胞增殖、迁移及小管形成的影响,并探讨PPARγ在猪体外血管生成中的作用。设置PPARγ激动剂组（5、10、15、20 μmol/L罗格列酮）、抑制剂组（5、10、15、20 μmol/L T0070907）及对照组,通过iCelligence细胞功能分析、划痕试验和Matrigel基质胶三维培养,构建猪体外血管生成的模型,模拟猪体内血管生成的环境,分别对猪血管内皮细胞的增殖、迁移、小管形成能力进行测定,同时根据NCBI已有的相关序列,应用Primer Premier 5.0软件设计PPARγ基因特异性引物,利用SYBR GreenⅠ实时荧光定量PCR检测PPARγ基因mRNA相对表达量,对PPARγ的体外作用效果进行验证。结果显示,5、10 μmol/L罗格列酮能促进猪血管内皮细胞增殖、迁移及小管形成,T0070907的抑制效果在试验浓度区间内（5~15 μmol/L）随浓度升高而加强,较高浓度（20 μmol/L）的两种药物均由于药物毒性的影响对细胞活动产生干扰。此外,5~20 μmol/L罗格列酮和5~20 μmol/L T0070907能分别提高和降低PPARγ基因mRNA相对表达量,且浓度趋势与增殖、迁移、小管形成的试验结果一致。综上所述,通过激活PPARγ可以对猪血管内皮细胞的增殖、迁移及小管形成产生促进效果,提示其在猪体外血管生成中具有积极作用,可为研究PPARγ对猪胎盘血管发生的影响提供参考依据。 相似文献
10.
《中国畜牧兽医》2017,(9)
试验旨在研究过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorsγ,PPARγ)对猪血管内皮细胞增殖、迁移及小管形成的影响,并探讨PPARγ在猪体外血管生成中的作用。设置PPARγ激动剂组(5、10、15、20μmol/L罗格列酮)、抑制剂组(5、10、15、20μmol/L T0070907)及对照组,通过iCelligence细胞功能分析、划痕试验和Matrigel基质胶三维培养,构建猪体外血管生成的模型,模拟猪体内血管生成的环境,分别对猪血管内皮细胞的增殖、迁移、小管形成能力进行测定,同时根据NCBI已有的相关序列,应用Primer Premier 5.0软件设计PPARγ基因特异性引物,利用SYBR GreenⅠ实时荧光定量PCR检测PPARγ基因mRNA相对表达量,对PPARγ的体外作用效果进行验证。结果显示,5、10μmol/L罗格列酮能促进猪血管内皮细胞增殖、迁移及小管形成,T0070907的抑制效果在试验浓度区间内(5~15μmol/L)随浓度升高而加强,较高浓度(20μmol/L)的两种药物均由于药物毒性的影响对细胞活动产生干扰。此外,5~20μmol/L罗格列酮和5~20μmol/L T0070907能分别提高和降低PPARγ基因mRNA相对表达量,且浓度趋势与增殖、迁移、小管形成的试验结果一致。综上所述,通过激活PPARγ可以对猪血管内皮细胞的增殖、迁移及小管形成产生促进效果,提示其在猪体外血管生成中具有积极作用,可为研究PPARγ对猪胎盘血管发生的影响提供参考依据。 相似文献
11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
13.
Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
14.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
15.
B. Cinar Kul N. Bilgen J.A. Lenstra O. Korkmaz Agaoglu B. Akyuz O. Ertugrul 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):449-453
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded. 相似文献
16.
Waruiru RM Thamsborg SM Nansen P Kyvsgaard NC Bogh HO Munyua WK Gathuma JM 《Tropical animal health and production》2001,33(3):173-187
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round. 相似文献
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The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host. 相似文献
18.
Troxel MT Vite CH Van Winkle TJ Newton AL Tiches D Dayrell-Hart B Kapatkin AS Shofer FS Steinberg SA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):850-859
The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma. 相似文献
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Evaluation of biohydrogenation rate of canola vs. soya bean seeds as unsaturated fatty acids sources for ruminants in situ 
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下载免费PDF全文 S. Pashaei T. Ghoorchi A. Yamchi 《Journal of animal physiology and animal nutrition》2016,100(2):211-216
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed. 相似文献