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1.
选用2月龄生长肉兔150只,随机分成5组,在基础日粮中添加不同水平的精氨酸(0,0.2%、0.4%、0.6%和0.8%),预试期7 d,正试期23 d.结果显示,日粮精氨酸水平对2～3月龄生长肉兔的平均日采食量、平均日增重、料重比、胸腺指数、脾脏指数、肝脏指数、免疫球蛋白M和G(IgM和IgG)、血清总蛋白(TP)、血清类胰岛素生长因子1(IGF-1)、血清生长激素(GH)和血清胰岛素(Ins)含量均无显著影响(P>0.05);血清免疫球蛋白A(IgA)含量随日粮精氨酸水平的增加极显著提高(P<0.01),日粮精氨酸水平对血清胆固醇(CHO)含量影响极显著(P=0.002 3),时血清尿素氮(SUN)含量影响显著(P=0.011 6).结果表明,2～3月龄生长肉兔日粮中已不需要再额外添加精氨酸. 相似文献
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高铜日粮对雏鸡血清中白介素-2含量的影响 总被引：6，自引：0，他引：6
1日龄艾维茵肉鸡健雏420只,随机分为7组,分别喂以对照日粮(Cu11mg/kg)和高铜日粮6周,以放射免疫法研究日粮高铜对雏鸡血清IL-2含量的影响。高铜Ⅰ组雏鸡血清IL-2含量升高,4、6周龄的IL-2含量与对照组比较差异显著(P<0.05);日粮铜含量超过300mg/kg,雏鸡血清IL-2含量程度不同地降低,与日粮含铜量呈负相关。结果表明:日粮含铜100mg/kg对雏鸡血清IL-2合成有明显的促进作用,日粮含铜300mg/kg及其以上对IL-2合成产生程度不同的抑制作用。 相似文献
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摘SSSS要：试验以育肥牦牛为研究对象,采用随机分组设计,试验选取了20头状况良好,体重差异小(180±20)kg的牦牛作为实验对象,随机分为2组,每组10头牦牛。让两组牦牛摄入不同蛋白水平日粮(低蛋白日粮组：13%;高蛋白日粮组16%)。研究分析了不同蛋白水平日粮对育肥牦牛血液生化指标、激素水平及生产性能的影响。结果表明：饲喂不同蛋白水平日粮对牦牛的生产性能产生了积极影响,试验组牦牛干物质采食量随日粮蛋白的水平的升高显著下降(P<0.05),试验指标中的的日增重、干物质采食量低蛋白日粮组显著均高于高蛋白日粮组组(P<0.05),高蛋白日粮组的饲料转化率相较低蛋白日粮组显著升高(P<0.05)。相较于低蛋白日粮组,高蛋白日粮组牦牛血液中的血糖(GLU)、甘油三酯(TG)、胆固醇(CH)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)浓度均显著高于低蛋白日粮组((P<0.05),游离脂肪酸NEFAl高蛋白日粮组均显著高于低蛋白日粮组(P<0.05)。血清中胰岛素(INS)、胰岛素样生长因子1 (IGF-1)、胰岛素样生长因子2 (IGF-2)的浓度随日粮蛋白水平的升高均有了显著升高,但牦牛血清中生长激素(GH)却随日粮蛋白水平的升高显著降低(P<0.05)。同时,经济效益数据表明低蛋白日粮组增重养殖效益更好,产生的经济利润最高。 相似文献
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为研究日粮能量水平对塞北乌骨鸡血清促性腺激素分泌的影响,试验选60只30周龄体重相近、来源相同、产蛋性能相似的塞北乌骨鸡,随机分为5组,每组3个重复,每个重复4只鸡。试验1组为对照组,饲喂基础日粮;试验2~5组分别饲喂能量降低5%和10%及提高5%和10%日粮。采用放射免疫分析方法(RIA)测定血浆促卵泡素(FSH)和促黄体素(LH)。结果表明,试验结束时,与试验1组相比,试验2组FSH含量差异极显著(P<0.01);试验2组与试验1、3、4、5组组间LH含量差异极显著(P<0.01)。提示,能量通过促性腺激素分泌影响生殖性能。 相似文献
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试验研究不同日粮结构和能量水平对后备期特种野母猪饲料养分消化率、血清生化指标的影响。通过采用2(日粮结构)×4(能量水平)因子设计,选用后备特种野母猪32头随机分为8组",玉米-豆粕"型和"40%的稻谷-玉米-豆粕"型日粮各设4个能量水平。结果表明:①养分表观消化率:不同日粮结构对各营养物质表观消化率的影响未达显著水平(P>0.05)。不同能量水平对试验猪只总能、粗蛋白、干物质的表观消化率的影响显著(P<0.05),对粗灰分的表观消化率影响不显著(P>0.05)。②生化指标:不同日粮结构对各生化指标影响均不显著(P>0.05)。不同能量水平对血清TP、血清Alb、血清Glo的影响达到了显著水平(P<0.05)。结论:后备期特种野母猪最适宜的日粮结构为"40%的稻谷-玉米-豆粕"型日粮,消化能为13.00 MJ/kg。 相似文献
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不同日粮结构和能量水平对后备期特种野母猪血清酶活指标的影响。通过采用2(日粮结构)×4(能量水平)因子设计,选用32头后备特种野母猪随机分为8组,玉米-豆粕型和40%的稻谷-玉米-豆粕型日粮各设4个能量水平。结果表明:不同日粮结构对各血清酶活性指标影响均不显著。不同能量水平对血清谷丙转氨酶和血清谷草转氨酶的影响达到了显著水平,对血清碱性磷酸酶和血清胆碱酯酶的影响不显著。后备期特种野母猪最适宜的日粮结构为40%的稻谷-玉米-豆粕型日粮,消化能为13 MJ/kg。 相似文献
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本试验在蛋鸡日粮中添加不同浓度的酵母硒,测定蛋鸡血清生化指标,旨在探究酵母硒对蛋鸡肝脏功能的影响。结果表明:日粮添加酵母硒对蛋鸡血清谷草转氨酶(Aspartate aminotransferase,AST)、谷丙转氨酶(Alanine aminotransferase,ALT)、总胆红素(Total bilirubin,TBIL)无显著影响;日粮添加酵母硒显著降低了血清总胆固醇(Total cholesterol,TC)含量,其中0.80 mg/kg的酵母硒效果最好,第28天比对照组下降了38.06%。认为日粮添加酵母硒没有对蛋鸡肝脏功能产生不良影响,且有保护心血管的作用。 相似文献
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《畜牧兽医学报》2017,(12)
旨在探讨不同淀粉类型日粮对育肥猪肠道消化吸收、血清生化指标和激素水平的影响。将90头((68±2.0)kg)阉公猪随机分配到3种日粮处理,每个处理5个重复,每个重复6头猪。采用纯的蜡质玉米淀粉(WMS)、非蜡质玉米淀粉(NMS)和豌豆淀粉(PS)作为淀粉来源来配合3种试验日粮,日粮的直链淀粉/支链淀粉比例分别为0.07、0.19和0.28。饲养28d后,每个重复选择2头猪(体重接近于每个重复的平均体重)称重,屠宰,取空肠黏膜和血液样品进行指标测定。结果表明:1)不同淀粉类型日粮对育肥猪空肠黏膜消化酶活性无显著影响(P0.05)。2)与WMS日粮相比,PS日粮显著下调感受甜味的味觉受体家族1的成员2和成员3(T1R2/T1R3)的mRNA表达水平(P0.05),而显著上调了可溶性载体家族1成员5(SLC1A5)的mRNA表达水平(P0.05);NMS日粮显著上调了可溶性载体家族7成员7(SLC7A7)的mRNA表达水平(P0.05);PS日粮和NMS日粮显著下调了钠/葡萄糖协同转运蛋白1(SGLT1)和葡萄糖转运蛋白2(GLUT2)的mRNA表达水平(P0.05)。3)在血清生化指标上,不同淀粉类型日粮对血液葡萄糖(Glu)含量并无显著影响(P0.05);与WMS日粮相比,PS日粮和NMS日粮显著降低了游离脂肪酸(FFA)含量(P0.05)。4)不同淀粉类型日粮对血清激素水平并无显著影响(P0.05)。本试验结果揭示,育肥猪饲喂高直支链比例淀粉日粮会下调甜味感受体、葡萄糖转运载体的mRNA表达水平,上调部分氨基酸转运载体mRNA表达水平,从而减弱肠道对葡萄糖的吸收,有利于对氨基酸的吸收。 相似文献
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Carstanjen B Sulon J Banga-Mboko H Beckers JF Remy B 《Domestic animal endocrinology》2003,24(1):31-41
This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine serum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine-specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet. 相似文献
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Insulin-like growth factor I (IGF-I) circulates in serum bound to a number of different binding proteins (BPs). With antibodies currently available, BPs must be dissociated and inactivated or removed from serum prior to measurement of IGF-I by radioimmunoassay (RIA). Serum samples which spanned a 13-fold range in IGF-I concentration were obtained from lactating dairy cows and used to develop conditions for assay of IGF-I with minimal interference from BPs. Removal of BPs from serum by acid-ethanol extraction resulted in interference in the RIA. Therefore, serum was incubated with 0.1 M glycyl-glycine HCl to inactivate BPs as suggested by Underwood et al. Time, temperature and pH were optimum when serum was incubated for 48 hr at 37 C, pH 3.7. Binding protein inactivation was evaluated by ability of glycyl-glycine incubated serum to reassociate with 125I-IGF-I. In addition, BPs isolated by gel filtration of glycyl-glycine incubated serum were tested for interference in the RIA. The concentration of IGF-I in serum where inactivated BPs were removed by acid gel filtration was compared to corresponding glycyl-glycine incubated serum. There was a 1:1 relationship which intersected at zero indicating that total IGF-I could be measured. Therefore, incubation of serum with glycyl-glycine is a reliable method for measuring total IGF-I in serum from dairy cows. 相似文献
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Comparing the values of progesterone in the blood of bitches as measured with a chemiluminescence immunoassay and a radioimmunoassay 
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下载免费PDF全文 A 125I radioimmunoassay (RIA) has long been used to determine the value of progesterone in serum or plasma of bitches but was discontinued in 2014. A chemiluminescence immunoassay (CLIA) gained prominence since 2003 to determine the value of progesterone in serum of bitches but the assay changed in 2012. This study assessed the agreement between progesterone values obtained with RIA in plasma (progRIA) and with the post‐2012 CLIA (progCLIA) in the serum of bitches. ProgCLIA was determined in 110 serum samples from 40 bitches in pro‐oestrus or early oestrus and compared to progRIA in plasma samples collected from the same bitches at the same time, where progRIA had a uniform distribution between 0.5 and 25 nmol/L. Two replicate analyses of each serum or plasma sample were simultaneously done in the same assay. For RIA and CLIA, the intra‐assay CVs were 5.85% and 6.70% and the interassay CVs 8.45% and 9.16%. For RIA and CLIA the progesterone values obtained with replicate analyses differed by as much as 11%–31% in 25% of samples. On average, the value of progCLIA was 85% of that of progRIA (95% CI 58%–112%, n = 110), with 88% of progCLIAs being lower than the progRIAs. This study shows that RIA and CLIA may yield replicate values that differ by as much as 11%–30% in about a quarter of samples analysed, necessitating replicate analyses if precise values are required. The study provides an equation by which to estimate progCLIA from progRIA. 相似文献
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Klein BS Squires RA Lloyd JK Ruge DR Legendre AM 《American journal of veterinary research》2000,61(5):554-558
OBJECTIVE: To assess whether dogs with blastomycosis produce antibodies against the WI-1 and A-antigens of Blastomyces dermatitidis and whether the antibodies are useful in serodiagnosis. SAMPLE POPULATION: 359 serum samples obtained from 245 dogs. PROCEDURE: 233 samples from 122 dogs with blastomycosis, and 1 sample each from 24 dogs with suspected blastomycosis, 51 control dogs without infection, and 48 healthy dogs from an enzootic region were obtained. Antibodies against WI-1 antigen were detected by radioimmunoassay (RIA). Serum samples were tested in parallel for antibodies against the A-antigen of B dermatitidis by commercial agar-gel immunodiffusion (AGID) in a reference laboratory. RESULTS: Antibodies were detected in 92% of infected dogs by RIA and in 41 % by AGID. For 29 serum samples that were obtained 11 to 1,545 days after diagnosis, antibodies were detected in 92% of samples by RIA and 7% by AGID. For 93 serial serum samples from 29 dogs with blastomycosis, the mean anti-WI-1 titer was 1:18,761 at the time of diagnosis, and decreased to a mean of 1:1,338 by 210 days after treatment was initiated. Of 24 dogs with suspected infection, antibodies were detected in 67% by RIA and 33% by AGID. Control dogs without blastomycosis had no detectable antibodies in either assay. Thus, sensitivity was 92% for RIA and 41 % for AGID, and specificity was 100% for both tests. CONCLUSIONS AND CLINICAL RELEVANCE: Anti-WI-1 antibodies are readily detected by RIA in dogs with blastomycosis. Titers become high, decline during treatment, and persist for months. Anti-A antibodies are sometimes detected with AGID, but these decrease quickly. 相似文献
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The radioimmunologic assay (RIA) was elaborated for a demonstration of serum antibodies to bovine leukosis virus. The procedure makes use of the viral antigen bond to the fixed phase of a polystyrene carrier. The method was compared with the ELISA method and pseudoneutralizing and immunodiffusion tests. High congruence of the results of the RIA and ELISA methods was achieved, making 95%. The RIA method is more sensitive than the immunodiffusion test. 相似文献
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Lurye JC Behrend EN Kemppainen RJ 《Journal of the American Veterinary Medical Association》2002,221(2):243-249
OBJECTIVE: To compare serum total thyroxine (T4) concentrations obtained with an in-house ELISA and a validated radioimmunoassay (RIA). DESIGN: Laboratory trial. SAMPLE POPULATION: 50 canine and 50 feline serum samples submitted for measurement of total T4 concentration with the RIA; samples were selected to represent a wide range of concentrations (< 6 to 167 nmol/L). PROCEDURE: Results of the ELISA and RIA were compared by calculating correlation coefficients, examining linearity, determining bias and precision, and evaluating clinical interpretations. RESULTS: Correlation coefficients for results of the 2 methods were 0.84 for the canine samples and 0.59 for the feline samples. Examination of bias plots revealed large variations in ELISA results, compared with RIA results. For the feline samples, the ELISA consistently overestimated total T4 concentration obtained with the RIA. When results of the 2 methods were categorized (low, borderline low, normal, borderline high, or high), results were discordant for 24 (48%) and 29 (58%) of the canine samples and for 18 (36%) and 28 (56%) of the feline samples (depending on whether borderline high ELISA results were considered normal or high). Reliance on results of the ELISA would have led to inappropriate clinical decisions for 31 (62%) canine samples and 25 (50%) feline samples. The ELISA coefficients of variation for the pooled canine and feline samples were 18 and 28%, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Substantial discrepancies between ELISA and RIA results for T4 concentrations were detected. Thus, we concluded that the in-house ELISA kit was not accurate for determining serum total T4 concentrations in dogs and cats. 相似文献
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Comparison of four serologic tests for the detection of antibodies to bovine leukemia virus 总被引：5，自引：0，他引：5
Four tests for detection of antibodies to bovine leukemia virus (BLV) were compared. The sera that were tested came from cattle in naturally infected commercial dairy herds, cattle that were infected under experimental conditions, and cattle in an isolated BLV-free herd. The tests that were compared included a radioimmunoprecipitation assay (RIA) with p24 antigen, a RIA with glycoprotein (gp) antigen, an agar-gel immunodiffusion (AGID) test with gp antigen, and a virus-neutralization (VN) test that was based on inhibition of BLV-induced syncytia in cell culture. Results of the 4 serologic tests agreed for 96.8% of the sera from cattle in commercial herds. The gp RIA detected the greatest number of positive sera (188); it was followed in turn by the p24 RIA (187), the VN test (183), and the AGID test (176). The gpd RIA titers of the 12 sera that gave negative AGID results were 175 or less. In RIA, the percentage of precipitation of labeled antigen by positive sera was almost always higher with gp antigen than with p24 antigen. Satisfactory sensitivity in the p24 RIA required the acceptance of a low level of antigen precipitation, 15%, as a positive test. In the gp RIA, however, almost all positive sera precipitated at least 50% of the labeled antigen. Nonspecific precipitation of antigen in the RIA by sera from BLV-free cattle ranged from 4% to 10%. Examination of sequential serum samples from 17 experimentally infected cattle showed that BLV antibody was first detected 2 to 8 weeks after inoculation. In 9 cattle, seroconversion was detected simultaneously by all of the tests. Results from the other 8 cattle indicated that seroconversion could be detected first by p24 RIA, followed by the gp RIA and the VN test. The longest interval between RIA seroconversion and AGID seroconversion was 10 days. Monthly tests of sera from 10 laboratory cattle that were infected by contact exposure showed that 7 animals seroconverted in all tests at the same time. Two cattle were positive first in RIA, but the next month they were also positive in the VN and AGID tests. One animal was positive in the RIA and the VN test for 2 months before antibody was detected by AGID. 相似文献
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Serum progesterone determination as an aid for pregnancy diagnosis in goats bred out of season 下载免费PDF全文
下载免费PDF全文 Fleming SA Van Camp SD Chapin HM 《The Canadian veterinary journal. La revue veterinaire canadienne》1990,31(2):104-107
Nubian does (n = 12) were bred by artificial insemination after induction of estrus with medroxyprogesterone acetate impregnated vaginal sponges and pregnant mare serum gonadotrophin injections during the anestrous season. Pregnancy status was predicted from serum samples collected 21 days following the last breeding and analyzed using 1) a commercial bovine milk progesterone enzyme immunoassay test (EIA), and 2) a radioimmunoassay progesterone (RIA) test. Both tests detected nonpregnancy (EIA 100%, RIA 80%) more accurately than pregnancy (EIA 66%, RIA 75%). Commercial bovine progesterone EIA kits have potential as rapid, inexpensive screening tests for nonpregnant does bred out of season. 相似文献
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ZS Perényi O Szenci PV Drion H Banga-Mboko NM Sousa B El Amiri JF Beckers 《Reproduction in domestic animals》2002,37(6):324-329
Pregnancy‐associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml – 1 mg/ml). Pepsinogen cross‐reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 μg/ml and 500 μg/ml concentrations, respectively. In the presence of pepsin, cross‐reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 μg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross‐react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross‐reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. 相似文献