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为研究妊娠早期子宫内交感神经对PCNA的调控效应,选取180~200日龄健康的性成熟Wistar雌性大鼠36只,随机分为对照组和处理组,处理组采用静脉注射6-OHDA损毁交感神经。采用免疫组化法和qRT-PCR法,观察和分析交感神经对妊娠早期大鼠子宫内膜组织学结构以及PCNA分布的影响。结果表明,PCNA阳性物质主要分布在固有层浅层的蜕膜细胞及肌层的外纵肌细胞核内存在。损毁交感神经后,子宫腺较正常的妊娠状态减少,腺上皮变薄,PCNA免疫阳性细胞减少,子宫内膜的蜕膜细胞及平滑肌细胞等的PCNA发生异常表达,特别是着床前的妊娠第4天(处理组)大鼠子宫腺数量减少,但腺上皮内PCNA免疫阳性细胞增强,子宫正常的组织学微环境被打乱,导致囊胚不能与子宫建立联系,出现着床数下降而排卵数基本正常的状况。免疫组化观察和mRNA相对表达量的统计分析均显示损毁支配子宫的交感神经后,导致子宫组织的蜕膜细胞及平滑肌细胞等的PCNA发生异常表达,打破了正常生理状态下子宫内膜发育与胚泡发育的同步性,影响妊娠早期的胚泡着床。 相似文献
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应用HE染色方法和免疫荧光组织化学技术对小鼠生后不同发育阶段子宫组织结构的发育以及极性调控蛋白Crb1的定位表达进行了研究,结果显示,随个体发育,子宫管腔及子宫腺腔不断扩大,子宫内膜上皮持续增厚、固有层内子宫腺逐渐发达;4周龄时子宫腔面出现纤毛,随发育进程越来越发达;在各发育阶段的小鼠子宫组织中均有Crb1的表达,随个体发育表达呈现先增强后减弱的趋势,在8周龄时达到最强;Crb1主要定位于子宫内膜上皮细胞和子宫腺上皮细胞的胞膜和胞质,提示Crb1可能与小鼠子宫内膜上皮细胞和腺上皮细胞的极性建立和维持有关。 相似文献
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牦牛发情周期子宫组织结构的观察 总被引：3，自引：2，他引：1
采用组织学和形态计量学的方法对发情周期牦牛子宫的组织结构及形态变化进行了观察。结果表明,牦牛子宫壁具有3层结构,分别为黏膜(子宫内膜)、肌层(子宫肌层)和浆膜(子宫外膜)。子宫内膜表面上皮和腺上皮在卵泡期为单层柱状上皮,在黄体期上皮高度明显增加,为假复层柱状上皮。表面上皮主要由分泌细胞及少量淋巴细胞组成。分泌细胞可分为亮细胞和暗细胞2种。亮细胞呈柱状,细胞体积较大,胞质弱嗜酸性;暗细胞呈两侧压扁的细条状,胞质强嗜酸性。腺上皮的细胞组成与表面上皮相似。PAS反应可见黄体期子宫内膜表面上皮、腺上皮和基质中阳性分泌颗粒较卵泡期的多。卵泡期固有层内偶见嵌套腺管、扩张腺管和弥散淋巴组织,但黄体期固有层内仅偶见嵌套腺管。此外,与卵泡期相比,黄体期子宫内膜和子宫肌层的厚度显著增加(P0.01)。黄体期子宫内膜腺管的平均直径、单个腺管的面积和周长、每平方毫米内腺管的总面积和总周长都较卵泡期的大,差异极显著(P0.01),而每平方毫米内腺管的数量差异并不显著(P0.05)。可见,牦牛子宫的组织结构在发情周期发生着有规律的变化。 相似文献
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猪胚胎的附植是通过广泛的细胞运动和重塑发生。胚胎的成功附植主要取决于胚胎延伸和滋养外胚层与子宫内膜上皮紧密连接的成功，这种紧密连接保证了孕体摄取其存活所必需的子宫腺上皮分泌物质。这些过程需要子宫和胚胎来源的细胞因子完成子宫内膜和滋养外胚层之间的细胞重塑，营造胚胎附植时的促炎微环境，维持附植阶段免疫系统的平衡，协调子宫和胚胎之间的相互作用等。本文主要综述了猪胚胎附植时期，由子宫和胚胎来源的细胞因子包括白细胞介素-1β(IL-1β)、干扰素（IFN）、成纤维细胞生长因子（FGF）、肿瘤坏死因子-α(TNF-α)、转化生长因子-β(TGF-β)发挥的作用及其作用机理，以期为胚胎附植及发育调控相关研究提供参考。 相似文献
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成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点 总被引：2，自引：0，他引：2
成纤维生长因子4(FGF4)是在哺乳动物胚胎发育过程中第一个被发现的FGF分子,在促进滋养层细胞的增殖方面具有广泛的作用。已经证实,FGF4是小鼠早期胚胎发育过程中的一个重要因子,异常表达会影响早期胚胎的发育,并影响小鼠胎盘的发育及功能。截至目前,有关FGF4在牛胎盘发育过程中的表达尚无报道。本试验运用石蜡切片HE、PAS、免疫荧光染色技术和实时荧光定量PCR(qRT-PCR)技术对妊娠早期牛胎盘组织结构特征及胎盘组织中FGF4的细胞定位和mRNA表达规律进行研究。结果显示,在所检测的各胎龄胎盘的羊膜、子叶及肉阜组织均检测到Fgf4 mRNA,各部位表达量依区域和孕龄而异;在牛胎盘块的单核滋养层细胞、滋养层巨细胞、肉阜上皮、子宫内膜上皮及子宫腺等部位的细胞质均观察到FGF4阳性反应,滋养层巨细胞及子宫腺上皮呈强染色。可见,在妊娠早期的牛胎盘组织存在FGF4及其mRNA,随着胎龄的增加其表达量在胎儿胎盘(子叶)表现为先降低而后增高的规律,而在母体胎盘(肉阜组织)则呈现先逐渐升高然后降低的趋势。 相似文献
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雌性哺乳动物在进入初情期（青春期）之后,其子宫内膜随着发情周期（月经周期）及妊娠建立等机体生理机能的转换,发生一系列周期性组织结构上的变化,而这种周期性变化主要与其本身血管周期性新生密切相关,血管新生则主要受血管内皮生长因子（VEGF）的调控。本文对雌性哺乳动物子宫内膜在发情周期、胚胎植入过程及胚胎发育过程及胎盘形成等的血管新生特点及VEGF的表达规律进行综述,以便系统地了解雌性动物子宫内膜的血管新生过程及调控机制,为提高雌性动物繁殖效率提供参考依据。 相似文献
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胚胎附植是一个复杂的生物过程，在哺乳动物的妊娠环节中至关重要，其发生受到很多转录调控因子的影响。长链非编码RNA(Long Non-coding RNA,lncRNA)是一类长度大于200核苷酸（nt）的转录本，基本不编码蛋白质，但其参与胚胎发育、肌肉发育、性别决定与分化等多种生物学过程和疾病的调控。本文简述lncRNA的研究现状，主要介绍了lncRNA通过影响子宫内膜容受性、调节蜕膜、调节子宫内膜增殖、调节激素水平等途径促进胚胎附植，以期为哺乳动物胚胎附植期中lncRNA的功能研究提供参考。 相似文献
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《中国兽医学报》2017,(7):1300-1308
为研究交感神经对妊娠早期子宫内DKK-1和Wnt-1的调控作用,选取180~200日龄健康的性成熟Wistar雌性大鼠,随机分为对照组和处理组,处理组采用静脉注射6-OHDA损毁交感神经,用免疫组化法和qRT-PCR法,观察和分析交感神经对妊娠早期大鼠子宫内膜的DKK-1和Wnt-1蛋白及mRNA相对表达量的影响。结果显示:6-OHDA损毁交感神经后,对卵巢排卵数无显著性影响,但子宫囊胚数量显著下降、囊胚发育延迟,导致子宫腔上皮和腺上皮细胞自分泌DKK-1和Wnt-1紊乱,呈现与正常状态相反的变化趋势。结果表明:在着床窗口期,交感神经通过影响DKK-1对Wnt-1信号分子的调控作用而影响囊胚着床、发育。 相似文献
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JC Dockweiler B Cossic CG Donnelly RO Gilbert E Buckles SH Cheong 《Reproduction in domestic animals》2017,52(1):174-178
A three‐year‐old intact female Old English sheepdog was presented for evaluation of infertility. A uterine biopsy was performed during dioestrus, and the microscopic appearance was inconsistent with progesterone stimulation; the glands were sparse, simple and failed to show coiling, while the glandular epithelium was cuboidal instead of columnar. There was very little evidence of glandular activity. Due to the inappropriate appearance of the glands for the stage of the cycle, immunohistochemistry for progesterone receptors was performed. No progesterone receptor‐positive immunoreactivity was identified in the endometrial luminal epithelium, glandular epithelium or stroma. Weak intranuclear immunoreactivity was identified within the smooth muscle cells of the myometrium. The absence of progesterone receptors within the endometrial glands is the most likely explanation for the abnormal appearance of the endometrium and for this bitch's infertility. To our knowledge, this is the first report of endometrial progesterone receptor absence in a bitch. 相似文献
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运用形态计量学方法对31头牦牛肉阜间区子宫腺的相关组织学数据进行了测定,并进行统计分析。研究发现,除了妊娠151~180 d,牦牛肉阜间区子宫腺上皮高度随着妊娠时间的增长极显著降低(P0.01);妊娠期子宫腺管周长和面积非常明显增大(P0.01);不同妊娠阶段之间体积密度差异极显著(P0.01),先增加后下降,而表面密度无显著差异(P0.05);除了妊娠151~180 d,扭曲腺管和管状腺管的比例随着妊娠时间的增长分别非常显著地增大或减小(P0.01);分泌腺管的比例在妊娠期间有明显变化(P0.05),牦牛肉阜间区还存在嵌套腺管。结果显示,妊娠期牦牛肉阜间区子宫腺形态结构有明显的变化,子宫腺变的更大、更长,形状更加扭曲。 相似文献
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A-M Tunón H Ekwall A Nummijärvi H Rodríguez-Martínez 《Reproduction in domestic animals》2000,35(5):221-227
X‐ray microanalysis was used to determine the occurrence of transudation as a source of intrauterine fluid in mares during oestrus. The content of some selected elements was studied in secretory vesicles of the epithelium of endometrial glands and in intraluminal fluid of healthy mares of varying age and parity. An in situ cotton tampon was used to absorb intrauterine fluid from 23 gynaecologically healthy mares during oestrus. Immediately after tampon withdrawal, endometrial tissue for biopsy was removed from the uterine body/horn junction while videoendoscopy was performed. The tissue specimens were frozen ultra‐rapidly against a copper surface chilled with liquid N2. X‐ray microanalysis of frozen fully hydrated endometrial samples and of microdroplets of intraluminal fluid (n = 20) was performed, using a SEM microscope equipped with a cryo‐stage and an X‐ray microanalysis system. The concentrations of sodium (Na), potassium (K), chlorine (Cl), sulphur (S), phosphorus (P) and calcium (Ca) in the epithelium of endometrial glands and in intraluminal fluid were compared with those in serum. There was a significant difference in the concentrations of all analysed elements between the secretory gland epithelium and the intraluminal fluid apart from S. The major elements in glandular epithelium were K and P. Although there was a significant difference in the concentrations of Na, Cl, S and Ca between the uterine fluid and serum, there was still a conspicuous resemblance in the relationship between the different elements in uterine fluid and serum, with Na and Cl being the dominant elements. Sulphur and especially Ca were present only in small amounts throughout. Neither age nor parity affected the elemental concentrations of Na, K, Cl, S, P and Ca in gland epithelium, uterine fluid or serum. It was concluded that serum transudation contributes to a great extent to the composition of uterine fluid, having a diluting effect upon the specific secretion of the uterine glands. 相似文献
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Chronic degenerative disease of the mare's endometrium is characterised by changes in the uterine glands, including cystic dilation, hyperplasia and periglandular fibrosis. Endometrial biopsies were taken from 23 mares with different grades of endometrial degeneration. Solid structures were identified within the lumina of the uterine glands and shown to be calcified by histochemical staining. Most of them were not homogenous but composed of a mixture of mineral and organic substances. Further examinations of these mineralised structures by immunohistochemical methods revealed the presence of the non-collagenous matrix proteins osteopontin, osteonectin and bone sialoprotein, which are known to be involved in calcification processes such as urolithiosis. Osteopontin and bone sialoprotein were identified within the calculi, frequently arranged in concentric layers. Osteonectin was the only matrix protein that was also present in the glandular epithelium. Osteocalcin was not found in either the calculi or the glandular epithelial cells. 相似文献
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Misirlioglu D Nak D Sevimli A Nak Y Ozyigit MO Akkoc A Cangul IT 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(5):225-229
The presence of oestrogen-alpha receptor (ER), progesterone receptor (PR), and HER-2/neu (c-erbB-2) oncoprotein in the uterine walls of 10 healthy cats and 20 subjects with cystic endometrial hyperplasia-pyometra (CEH-P) were evaluated. Lesions were graded according to the severity of cystic dilation, hyperplasia and inflammation, and were classified as normal, mild uterine hyperplasia and severe uterine hyperplasia. The ER, PR and c-erbB-2 expression in the endometrium, glandular epithelium, stromal fibroblasts and myometrial smooth muscle cells was quantified by immunohistochemistry. The ER, PR and c-erbB-2 staining patterns differed between normal uteri and uteri with CEH-P. The ER expression was tended to be higher in the endometrial surface and glandular epithelium in the severe hyperplasia group (P > 0.05) and significantly lower in the mild hyperplasia cases compared with normal endometrium (P < 0.05), whereas the PR expression in both severe and mild hyperplasia cases tended to be higher in stromal cells and glandular epithelium than those in the normal uteri. C-erbB-2 immunoreactivity was observed only in the endometrial surface and glandular epithelium of the uterine wall and immunostaining was found to be highest in cases with severe hyperplasia. As a conclusion, we suggest that c-erbB-2 oncoprotein may play a role in the pathogenesis of the CEH together with the ER and PR in cats, and that ER does not have a role in the mechanism of pyometra, whereas PR plays a role in the pathogenesis of both CEH and pyometra. 相似文献
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本研究旨在探明犬子宫和卵巢在正常发情期与患子宫蓄脓时，其组织结构及乳铁蛋白（lactoferrin，LF）表达的变化特征。应用Masson’s、VVG、PAS组织化学染色方法观察乏情期、发情期、患子宫蓄脓时犬子宫及卵巢的组织结构特点，用免疫组织化学SP法观察LF的分布特征。结果显示，正常发情期犬：乏情期、发情期子宫内膜肌层厚度比分别为0.762 0、0.924 3；乏情期子宫固有层中胶原纤维含量大于发情期，发情期卵巢中胶原纤维含量大于乏情期；子宫血管层及卵巢血管内弹性膜清晰完整；子宫腺在乏情期时浅层管腔小，深层较大、腺管上皮为单层柱状上皮、上皮细胞及管腔内PAS阳性反应较强，发情期子宫腺管腔变大、腺管上皮为单层立方上皮、上皮细胞及管腔内有PAS阳性反应；子宫黏膜上皮在乏情期和发情期均为单层柱状上皮，但胞核位置不同，乏情期胞核位于中央，发情期胞核位于顶部。患子宫蓄脓犬：子宫内膜肌层厚度比为1.615 0；子宫固有层和卵巢中胶原纤维含量少于正常发情期；子宫腺管腔大，且形状不规则，管腔内有炎性细胞浸润，腺管上皮为单层立方上皮，有淋巴细胞位于基膜，上皮细胞及管腔内PAS阳性反应较弱；子宫血管层及卵巢血管内弹性膜较正常发情期变薄，且有断裂现象；子宫黏膜上皮为单层柱状上皮，胞核位于基底。LF在乏情期子宫腺上皮和卵巢中的表达水平高于发情期，而在子宫黏膜上皮中发情期的表达水平高于乏情期。患子宫蓄脓时犬子宫和卵巢中LF的表达水平均较低。综上表明，犬正常发情期与患子宫蓄脓时的子宫和卵巢组织结构特点显著不同，LF的表达水平也存在差异。 相似文献
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A quantitative study of the histological morphology of the endometrium of normal and barren mares. 
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下载免费PDF全文 The density of uterine glands, height of surface epithelium, numbers of hemosiderin laden macrophages, inflammatory cells and layers of periglandular fibrosis were evaluated in uterine biopsies from 40 mares. These features were found to be highly variable in normal equine endometrium. Minor pathological changes appeared to be masked by this normal variability. Atrophy of uterine glands was recognized in mares which had been barren for more than three years. No significant differences were found between barren and normal mares in the height of epithelium, number of hemosiderin laden macrophages, inflammatory cells or layers of collagen surrounding glands in the superficial portion of endometrium. The number of layers of fibrosis surrounding glands in the deep part of lamina propria were found to be correlated with years of barrenness. This finding appears to have prognostic potential. 相似文献
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The test was aimed to study the expression of nuclear protein 1 (Nupr1) mRNA in mouse uterus during early pregnancy.The method of in situ hybridization was used to investigate Nupr1 mRNA expression in animal models that included early pregnancy,pseudopregnancy,delayed implantation and activation,artificial decidualization and hormonal treatments.The relative expression level of Nupr1 mRNA was detected in early pregnancy and pseudopregnancy using Real-time PCR.During mouse early pregnancy,the signal of Nupr1 mRNA was detected in luminal epithelium and glandular epithelium during the 1st to 4th day and in the decidua area during the 5th to 8th day.Nupr1 mRNA was mainly expressed in the luminal epithelium and glandular epithelium of mose uterus on the 1st to 5th day of pseudopregnancy.The signal was detected in luminal epithelium and glandular epithelium of the mouse uterus in the delayed implantation,which was similar to the results of early pregnancy on the 4th day.The signal was detected in decidua in the model of delayed activation,which was similar to the results of early pregnancy on the 5th day.The expression of Nupr1 mRNA in the model of artificial decidualization was detected in decidua area.In the control of artificial decidualization the slight signal appeared in luminal epithelium and glandular epithelium of the mouse uterus.After treated with oestrogen (E2) the signal appeared in luminal epithelium and glandular epithelium of the mouse uterus,and the signal was enhanced.After treated with both of E2 and progesterone (P4), the expression of the signal was not changed significantly.Real-time PCR result showed that the relative expression on the 2nd day was higher than other days in early pregnancy and pseudopregnancy.The results indicated that the expression of Nupr1 mRNA in mouse uterus was related to the process of mouse early pregnancy.The expression of signal in luminal epithelium and glandular epithelium of the mouse uterus might be regulated by hormones.Nupr1 mRNA expression in uterine stroma was associated with decidualization and active blastocysts. 相似文献
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G Galabova M Egerbacher JE Aurich M Leitner I Walter 《Reproduction in domestic animals》2003,38(5):415-420
Although cyclic changes of the endometrium in dogs involving both stromal and glandular compartments have been described, the fate of the surface epithelium after progressive growth and secretion is still unclear. In the present study, uteri of 43 healthy bitches in metoestrus and anoestrus were examined macroscopically and histologically. Tissue biopsies were taken from three different locations (cranial and middle parts of uterine horns and bifurcation). The stage of the oestrous cycle was determined by evaluation of progesterone and oestradiol levels in plasma hormone and was also confirmed clinically. Crypts formed in the luteal phase were covered with a columnar epithelium which gradually underwent fatty degeneration. In addition, the stromal part of the crypts disappeared and finally, in early anoestrus, epithelial sheaths desquamated and shed off into the uterine lumen. The surface epithelium was replaced by new cuboidal cells proliferating and migrating from the glandular openings. These findings were confirmed by oil red O staining and immunohistochemical detection of proliferation with Ki‐67 marker. 相似文献