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1.
丛枝菌根对三叶草根际磷酸酶活性的影响 总被引：8，自引：0，他引：8
以三叶草为材料,利用三室隔网培养方法,探讨了取自肥料长期定位试验中多年施用与不施用有机肥的田间小区土壤上,接种菌根菌（G.mosseae）对根际土壤酸性和碱性磷酸酶活性的影响。植物生长9周后,收获测定菌丝生长室土壤酸性磷酸酶和碱性磷酸酶活性,并对磷酸酶产生位点进行细胞化学定位。结果表明,接种丛技菌根菌对根际土壤酸性和碱性磷酸酶活性均有增强作用,但作用程度在有机服小区土壤上要大于无机肥小区土壤。根际土壤酸性磷酸酶原位化学定位结果表明,菌丝周围有明显的酸性磷酸酶的反应产物,说明报外丛枝菌根菌丝能直接向外分泌磷酸酶。 相似文献
2.
【目的】蚯蚓和丛枝菌根真菌处于不同的营养级,但在促进植物生长和提高土壤肥力等方面却都发挥着积极作用。研究蚯蚓菌根互作及其对玉米吸收土壤中的氮、磷养分的影响,可为提升土壤生物肥力及促进农业的可持续发展提供理论依据。【方法】本研究采用田间盆栽方式,以玉米为供试作物,研究蚯蚓(Eisenia fetida)与丛枝菌根真菌(Glomus intraradices)互作及其对玉米养分吸收的影响。试验设置P 25和175 mg/kg两个水平。每个磷水平进行接种与不接种菌根真菌以及添加与不添加蚯蚓,共8个处理。调查了玉米生长、养分吸收以及真菌浸染和土壤养分的有效性。【结果】两个磷水平下,蚯蚓和菌根在增加玉米地上部和根系生物量方面有显著正交互作用(P0.05)。接种菌根真菌的各处理显著增加了玉米的侵染率及泡囊丰度、根内菌丝丰度等菌根指标。同时添加蚯蚓和接种菌根真菌的处理(AM+E)显著提高了菌根的侵染率、菌丝密度、丛枝丰度和根内菌丝丰度但是泡囊丰度有所下降。两种磷水平下,AM+E处理玉米地上部和地下部含氮量和含磷量均显著高于其他三个处理。在低磷条件下,地上部氮磷总量的增加分别是添加蚯蚓和接菌的作用;而地下部磷总量的增加主要是菌根真菌的作用。在高磷条件下,单加蚯蚓显著增加玉米氮磷的总量,而接种菌根真菌对玉米氮磷吸收的影响未达显著性水平。在高磷条件下,单加蚯蚓的处理显著提高玉米地上地下部生物量(P0.05),而单接菌的处理效应不显著,蚯蚓菌根互作通过提高土壤微生物量碳、氮实现对玉米生长和养分吸收的调控。在低磷条件下,单接菌显著提高了玉米的生物量(P0.05),单加蚯蚓的处理具有增加玉米生物量的趋势。菌根真菌主要促进玉米对磷的吸收,蚯蚓主要矿化秸秆和土壤中的氮磷养分增加土壤养分的有效性,蚯蚓菌根互作促进了玉米根系对土壤养分的吸收并形成氮磷互补效应。【结论】无论在高磷还是低磷水平下,蚯蚓菌根相互作用都提高了玉米地上地下部生物量、氮磷吸收量同时提高了土壤微生物量碳、氮。蚯蚓菌根相互作用对植物生长的影响取决于土壤养分条件。在高磷条件下(氮相对不足),蚯蚓菌根互作通过调控土壤微生物量碳、氮调控玉米生长和养分吸收。低磷条件下,菌根主要发挥解磷作用,蚯蚓主要矿化秸秆和土壤中的氮素,蚯蚓和菌根互补调控土壤中氮、磷,从而促进植物的生长和养分吸收。 相似文献
3.
丛枝菌根真菌菌丝对土壤微生物群落结构及 多氯联苯降解的影响 总被引：2，自引：0，他引：2
以摩西球囊霉(Glomus mosseae)为供试菌种,在光照培养箱内利用分室根箱研究丛枝菌根真菌菌丝对多氯联苯(polychlorinated biphenyls,PCBs)污染土壤的修复效应及其机理。试验设置接种丛枝菌根真菌的处理以及不接种的对照,选用美国南瓜(Cucurbita pepo L.)为供试植物,在南瓜生长40天后将接种菌根真菌处理的菌丝室土壤从尼龙网向外水平分为4层取样,测定PCBs及磷脂脂肪酸含量。结果表明:菌丝可以穿越尼龙网影响菌丝室土壤,且距离尼龙网越远菌丝量越低;菌丝显著促进了土壤微生物量(P0.05),并改变了不同土层土壤微生物群落结构;接种菌根真菌处理各土层PCBs降解率为35.67%~57.39%,均显著高于对照的17.31%,相关分析结果表明土壤三氯、四氯联苯以及PCBs总量与菌丝量呈极显著负相关(P0.01);菌丝际土壤微生物量,特别是细菌生物量与土壤三氯联苯含量呈显著负相关(P0.05)。可见,菌丝通过影响菌丝际土壤微生物群落结构及生物量,促进三氯及四氯联苯降解,从而提高土壤PCBs修复效率。 相似文献
4.
丛枝菌根真菌（AMF）在土壤与植物的磷素循环中发挥着关键的作用。采用盆栽实验研究了丛枝菌根真菌群落对白三叶草植株生物量、磷吸收和土壤磷酸单酯酶活性的影响。结果表明,接种不同AMF群落均能显著地促进白三叶草植株的生长及其对磷素的吸收,提高根际土壤磷酸单酯酶的活性。Mnp处理中,白三叶草生物量最大,白三叶草总生物量、茎叶生物量和根系生物量分别比对照处理（-M）提高64.48%、61.48%和84.91%。不同菌根处理中,Mck处理显著地提高白三叶草磷吸收和土壤磷酸单酯酶活性,白三叶草磷吸收总量和茎叶磷吸收量分别比对照（-M）提高107.18%和91.91%,土壤碱性磷酸单酯酶和酸性磷酸单酯酶活性相对对照（-M）分别提高54.33%和138.43%。碱性磷酸单酯酶活性与AMF群落中的Acaullospora属孢子数呈显著的正相关关系,而酸性磷酸单酯酶活性则主要受Paraglomus属孢子数的影响。说明接种AMF群落可显著地影响土壤的磷酸单酯酶活性,从而影响白三叶草的生长及其对磷素的吸收。 相似文献
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6.
丛枝菌根(AM)真菌对土壤中阿特拉津降解的影响 总被引：4，自引：0，他引：4
于盆栽高粱(Sorghum,龙杂一号)条件下研究了丛枝菌根(AM)真菌根内球囊霉(Glomus intraradices,GI)和摩西球囊霉(Glomus mosseae,GM)降解土壤中阿特拉津的效用。结果表明,阿特拉津(浓度为50 mg/kg)污染土壤中,供试AM真菌都能够侵染高粱根系形成菌根,而且GM比GI侵染效果好,最高侵染率可达到90.5%,显著提高了植株的生物量。接种AM真菌后土壤中阿特拉津的残留浓度显著低于不接种对照处理,并且接种GM比GI对阿特拉津的降解效果显著。接种GM处理的土壤中阿特拉津最高降解率达到了91.6%,其中菌根效应占22.6%。接种AM真菌的宿主植物根际土壤中微生物数量多于不接种处理,且GM优于GI处理,说明AM真菌能促进根际微生物的繁殖。此外,接种AM真菌后能显著增加土壤中脲酶活性,但对过氧化氢酶活性影响不显著。认为GM是一株比较理想的修复阿特拉津污染土壤的AM真菌。 相似文献
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8.
不同AM菌根菌分泌的磷酸酶对根际土壤有机磷的影响 总被引：18，自引：1，他引：18
以三叶草为材料,利用3室隔网培养方法,研究了4种AM菌根菌侵染三叶草后对根际土壤酸性和碱性磷酸酶活性以及菌丝酶活性对土壤有机P的影响。结果表明,接种AM菌根菌 (9周) 对根际土壤酸性和碱性磷酸酶活性均有增强作用,但作用强度主要取决于菌丝在土壤中的生长状况,Glomus属菌根菌在整个菌丝室 (0~6cm) 都影响土壤磷酸酶的活性,其活性在整个菌丝室中都比Gigaspora的高。同一属不同种的根际土壤磷酸酶活性差异不大。AM菌根根际土壤磷酸酶对土壤有机P的降解有很强的促进作用。 相似文献
9.
蚯蚓菌根互作对土壤酶活、 甘薯根系生长及养分吸收的影响 总被引：2，自引：0，他引：2
【目的】蚯蚓和丛枝菌根真菌处于不同的营养级,但在促进植物生长和提高土壤肥力等方面却都发挥着积极作用。单独对土壤微生物或土壤动物的研究较多,但对土壤微生物与土壤动物之间相互作用的研究很少。因此研究它们对土壤和植物生长的作用可为挖掘土壤生物的潜力和提高土壤生物肥力提供依据。【方法】采用盆栽试验,研究了蚯蚓(Eisenia fetida)与丛枝菌根真菌(Rhizophagus irregularis)互作对甘薯生长和养分吸收的影响。试验采用两因素完全随机试验设计,分为接种和不接种菌根真菌及添加和不添加蚯蚓。试验共4个处理: 不加菌根和蚯蚓(CK); 接种菌根真菌(AM); 添加蚯蚓(E); 添加蚯蚓和菌根真菌(E+AM),每个处理4次重复。调查了甘薯养分吸收、 根系形态及土壤养分变化,采用Canoco4.5软件对土壤生物与植物对应关系进行RDA (redundancy analysis)分析。【结果】接种菌根真菌显著提高了甘薯地上和地下部生物量(P0.05),而添加蚯蚓的处理仅提高了甘薯地上部生物量。同时添加蚯蚓和菌根的处理显著提高了甘薯地上地下部生物量,并且高于其他三个处理(P0.05)。与对照相比,接种菌根真菌显著提高了土壤磷酸酶活性(P0.01),增幅近一倍; 同时提高了土壤磷的植物有效性,土壤有效磷含量下降了30%左右。添加蚯蚓后土壤脲酶活性从5.45 mg NH+4-N/g显著增加到8.71 mg NH+4-N/g,土壤碱解氮的含量从5.82 mg/kg显著增加到6.89 mg/kg (P0.05)。RDA分析表明蚯蚓菌根互作对甘薯地上和地下部氮磷含量、 根表面积、 根体积、 根平均直径和根尖数均存在显著的正交互效应。蚯蚓菌根互作通过调控土壤酶和改变土壤养分有效性促进甘薯对土壤氮磷养分的吸收。【结论】蚯蚓(Eisenia fetida)通过调控土壤脲酶和碱性磷酸酶增加了土壤中氮磷的有效性从而促进甘薯地上部生长。丛枝菌根真菌(Rhizophagus irregularis)通过调控土壤磷酸酶和增加植株地上地下部吸磷量从而促进甘薯生长。添加蚯蚓或接种菌根真菌均能增加根系吸收面积和根体积从而促进甘薯对养分的吸收。蚯蚓和菌根真菌相互作用通过调控土壤酶和改变土壤养分有效性以及促进根系发育从而互补的促进甘薯养分吸收和生长。 相似文献
10.
菌根与根瘤菌联合应用对复垦矿区根际土壤环境的改良后效 总被引：2，自引：0，他引：2
为了进一步确定微生物复垦对土壤的持续修复能力,在神东矿区活鸡兔开采沉陷试验基地,接种丛枝菌根真菌和大豆根瘤菌改良农用地土壤,已取得了较好的微生物复垦效果,经过2 a的退耕撂荒以后,对微生物复垦后效进行系统地研究。研究结果表明,撂荒后,接菌小区根外菌丝密度、根系侵染率和pH值均显著高于对照小区,分别比对照提高了90%、52%和1.3%;接菌小区细菌数量、微生物总量、有效磷含量、电导率和酸性磷酸酶活性也明显高于对照小区,但差异不显著。撂荒后与撂荒前相比,土壤呈退化趋势,但接菌小区菌丝密度几乎增加了1倍,差异显著,同时侵染率、细菌数量和酸性磷酸酶活性均明显升高,差异不显著;而在对照小区中,菌丝密度稍有增加,侵染率、细菌数量和酸性磷酸酶活性均明显降低,且差异不显著。利用丛枝菌根真菌与大豆根瘤菌联合作为绿色环保肥料,能够有效改善根际土壤环境,减轻土壤的退化程度,在弃耕状态下也能发挥良好的效用,对土地贫瘠的开采沉陷地进行生态修复具有重大意义。 相似文献
11.
An alkaline phosphatase in the intraradical hyphae of arbuscular mycorrhizal fungi was found to be closely related to an
improvement of plant growth. To detect the phosphatase activity in a crude extract of mycorrhizal roots, phosphatase isozymes
in mycorrhizal and non-mycorrhizal onion roots were compared with those in Gigaspora margarita by electrophoresis. A mycorrhiza-specific band was found when the phosphatase was stained under alkaline conditions. To clarify
the origin of this phosphatase, the phosphatase extracted from intraradical hyphae was also compared with the phosphatase
from mycorrhizal roots by electrophoresis. The intraradical hyphae was isolated from mycorrhizal roots by enzyme digestion
followed by Percoll gradient centrifugation. The soluble protein was extracted from the hyphae by ultra-sonication after treatment
with chitinase. A phosphatase in the hyphal soluble protein showed a similar, but slightly higher, relative mobility on the
gel, compared with the mycorrhiza-specific phosphatase from roots. By adding the hyphal extract to the root extract, the relative
mobility of the mycorrhiza-specific phosphatase was slightly changed and became identical to that of the phosphatase in the
hyphae. This indicated that the specific band of phosphatase found in the crude extract from mycorrhizal roots was of intraradical
hyphal origin.
Received: 16 April 1997 相似文献
12.
《Communications in Soil Science and Plant Analysis》2012,43(17):2106-2122
ABSTRACTThis work was conducted to study phosphorus (P) efficiency of two maize genotypes (Zea mays, L.) in calcareous soil grown in potted soil with two levels of P in soil by adding 40 and 270 mg P/kg soil. Half of the pots were inoculated with arbuscular mycorrhizal fungi (AMF) (Rhizoglomus irregulare). The maize genotypes were harvested two times at 35 and 50 days after transplanting. The plant dry matter, root length and Plant P uptake of maize genotype Hagen 1 without mycorrhizal fungi (AMF) increased significantly compared with Hagen 9 at a low P level. In contrast, there was no significant difference between two maize genotypes inoculated with AMF under the same P level. The predicted value increased rapidly with increasing P levels from about 70% up to 97% in both maize genotypes with and without mycorrhizal fungi. At a low P level, the mycorrhizae hyphae contributed by about 31.6% and 30.2% of the predicted total P uptake in maize genotype Hagen 1 and Hagen 9, respectively. The results of this study suggested that the P-inefficient genotype Hagen 9 improved with inoculation with mycorrhizal fungi under a low P level at the same conditions of this experiment. Also, root growth system and mycorrhizal hyphae length would be a suitable plant parameter for studying P efficient maize genotypes, especially under limited P supply. The current study clearly pointed out that the mechanistic simulation model (NST 3.0) provides useful tools for studying the role of AMF in P uptake of plant. 相似文献
13.
In terrestrial ecosystems, plants are frequently in symbiosis with arbuscular mycorrhizal fungi (AMF) with mineral nutrients and photosynthesis carbon exchanges in between. This research sought to identify the effects of phosphorus (P) levels on the nitrogen (N) uptake via extraradical mycelium (ERM) and the mycorrhizal growth response (MGR) of maize plants within the AMF symbiosis. Pots were separated into root compartments and hyphae compartments (HCs) with two layers of a 30‐μm mesh membrane and an air gap in between, where only hyphae could pass through, to avoid both N diffusion and root growth effects. Maize plants were inoculated with Rhizophagus irregularis with different N fertilization in HCs under two different P fertilization levels. Our results indicated that a strong increase in MGR with low‐P fertilization. The same tendency was not observed with high‐P fertilization, although both had a large increase in P concentration as a potential source of growth in shoot tissue of mycorrhizal plants. Substantial effects (10.5% more N) were observed in the case of high‐P availability for the host plants from ERM fed with N, whereas under low‐P conditions ERM may prioritize P uptake rather than N uptake. The AM fungi increase the uptake of N and P, which are most limiting in the soil with fewer forces from soil resources. In addition, there was still more P accumulated than N due to the high N for ERM with high‐P supply. Low N in HCs corresponded with a lower colonization rate in roots but with high hyphae density in HCs; this result suggest that N and P availability might change the ratio of extraradical to intraradical hyphae length. 相似文献
14.
AM 菌根真菌诱导对提高玉米纹枯病抗性的初步研究 总被引：13，自引：1，他引：13
试验研究玉米接种摩西球囊霉后对纹枯病抗性反应的结果表明,接种摩西球囊霉能明显减轻玉米纹枯病的发病率和病情指数,减轻病害。接种摩西球囊霉还能促进玉米营养生长,但立枯丝核菌侵袭会降低菌根的侵染率,表明摩西球囊霉与立枯丝核菌间存在相互作用。 相似文献
15.
The effect of Bacillus thuringiensis (B.t.) inoculation on plant growth and on the intra- and extraradical mycorrhizal development of lettuce roots colonized by Glomus mosseae or Glomus intraradices was examined in an inert, soil-less substrate. Histochemical determination of succinate dehydrogenase (SDH) and alkaline phosphatase (ALP) activities which indicate active fungal metabolism was carried out at two phosphorus (P) levels. The presence of B.t. increased extra- and intraradical colonization [measured as frequency (%F), intensity (%I) and percentage of arbuscules (%A)] for both arbuscular mycorrhizal fungi (AMF) rather than plant growth or nutrition regardless P level. Under the lowest level of P fertilization, B.t. enhanced to a similar extent the extra- and intraradical development of both endophytes, but the proportion of fungal tissue showing SDH or ALP was increased in G. intraradices-colonized plants. [SDH: 458% (M) and 512% (A); ALP: 358% (M) and 300% (A)]. P supply decreased G. intraradices colonization to a higher extent than G. mosseae. Nevertheless, the totality of G. intraradices structures developed in P-amended medium showed intraradical o extraradical activity, while in G. mosseae-colonized roots, SDH and ALP activities highly decreased relative to fungal tissue determined by TB staining as affected by P. Our results show that bacterial inoculation compensates the negative effect of P on the intraradical fungal growth and vitality. P amendment reduced in a higher extent G. intraradices infection intensity (non-vital and vital staining) and G. mosseae activity (ALP staining). Thus, big differences in the proportion of SDH-active infection showing ALP activity in mycelium developed by each endophyte were noted at the highest P level. Physiological plant parameters such as photosynthetic activity did not explain specific changes on each arbuscular-mycorrhizal fungus as affected by P or B.t. inoculation. The increased extraradical mycelium development and metabolic fungal activity as a result of B.t. inoculation positively affected N and P plant content and photosynthetic rate in G. intraradices-colonized plants under the lowest P conditions. In general, the increased metabolically active fungal biomass in co-inoculated plants was irrespective of P level and was not related to the P plant uptake from the inert soil-less substrate. These results show the bacterial effect increasing the physiological and metabolic status of AM endophytes, which not only confirms but also extends previous findings on arbuscular mycorrhizae-bacteria interactions. The present study emphasizes the ecological and practical importance of rhizosphere free-living bacteria as mycorrhizae-helper microorganisms. 相似文献
16.
M. F. Allen C. Figueroa B. S. Weinbaum S. B. Barlow E. B. Allen 《Biology and Fertility of Soils》1996,22(4):287-292
Oxalate crystals and elements binding to the surfaces of mycorrhizal fungal hyphae were examined using scanning electron
microscopy coupled with X-ray analysis of elemental composition. Mycorrhizae from the arid zone vegetation types in southern
California were examined including chaparral, riparian oak woodlands, coastal sage, grasslands, and deserts. Only mat-forming
ectomycorrhizal hyphae, such as Hysterangium separabile, were found to produce oxalate crystals. None of the arbuscular mycorrhizal fungal hyphae (Glomus spp. and Acaulospora elegans) examined had crystal structures associated with them. The hyphae of Hysterangium separabile without crystals did not show the Ca peaks that were present when the crystals existed nor did the arbuscular mycorrhizal
fungal hyphae have the Ca peaks. The elimination of arbuscular mycorrhizae using benomyl did not affect soil P or oxalate.
These data indicate that there are some fundamental differences in chemical exudation between mycorrhizal fungi that could
affect P uptake and cycling in arid ecosystems.
Received: 7 December 1994 相似文献
17.
Abou El Seoud I. I. R. M. El Adly M.W. Sadik F.S. Moghanm 《Communications in Soil Science and Plant Analysis》2020,51(8):1006-1023
ABSTRACT This experiment aimed to study phosphorus efficiency of six wheat genotypes (Triticum aestivum) inoculated with arbuscular mycorrhizal fungi (AMF) and to quantify the contribution of root and mycorrhizal hyphae length to P uptake by using NST 3.0 model. The results showed that all wheat genotypes with AMF (except V4) attained more than 80% of the maximum shoot yield. NST 3.0 predicted approximately 49% and 30% of observed P uptake for V4 with and without mycorrhizae, respectively, at the lowest P level. Additionally, the predicted values of P uptake increased rapidly with increasing P levels by up to 90% and 89% with and without mycorrhizae, respectively, at the highest P level. The model predicted 58% and 43% of the observed P uptake for V6 with and without mycorrhizae, respectively, at the lowest P level and increased up to 98% and 95% respectively at the highest P level. Soil P depletion zones of plants without mycorrhizal fungi (V4 and V6) did not extend as far as those of plants with mycorrhizal fungi. In conclusion, we recommend that V6 (Gemmeiza12) is suitable for growth in calcareous soil with or without mycorrhizal fungi inoculation (highly P efficient). The results of this study suggest that root growth and mycorrhizal hyphae length are the main parameters suitable for selecting P-efficient wheat genotypes, especially under limited P supplies. The current study clearly shows that (NST 3.0) model provide useful tools for studying the role of (AMF) and root length in plant P uptake. 相似文献
18.
为研究接种丛枝菌根真菌(AMF)对玉米秸秆降解的影响,利用玉米秸秆为材料制成网袋,采用盆栽试验,以玉米(Zea mays L.)为宿主植物,分别接种Glomus intraradices和Glomus mosseae,于30,40,50,60d时收获后分析玉米秸秆降解量和C、N释放量,并运用Olson的指数模型Bt/B0=e-kt计算玉米秸秆及C、N的降解系数。数据表明,接种G.intraradices、G.mosseae显著提高了玉米秸秆降解量和降解系数,与不接种处理相比,分别高出5.21%,6.26%。C释放量、碳素降解系数也明显增加。接种处理减少了N释放量,且氮素降解系数随时间延长而下降。接种处理玉米秸秆的C、N降解系数不同直接反映了其降解速度的差异,进而影响了玉米秸秆的C/N,使秸秆更易于降解。研究结果显示出丛枝菌根真菌在生态系统氮循环中具有重要意义。 相似文献
19.
【目的】多聚磷是丛枝菌根内磷的主要贮存形式,定性、定量观察多聚磷对于解析菌根中磷代谢具有重要意义。随着植物体内越来越多的参与菌根真菌与寄主植物之间营养交换过程的基因被鉴定,迫切需要进一步提高根内菌根共生结构和多聚磷累积的染色和定位分析技术。【方法】本研究利用丛枝菌根真菌Glomus mosseae侵染的大豆植株,采集新鲜根样制片,一部分薄根片利用低浓度荧光染料麦胚凝集素,室温染色30 min,在波长488 nm的蓝光激发下使用荧光显微镜观察拍照;另一部分薄根片利用荧光染料4’,6-二脒基-2-苯基吲哚二盐酸盐(DAPI)进行染色,在波长405 nm紫外光激发下观察并拍照;进一步取新鲜制备的薄根片,先后用以上两种荧光染料进行染色,分别在波长405 nm和488 nm的激发光下观察并拍照,完成了菌根共生结构和多聚磷的共定位。【结果】1)使用荧光染料麦胚凝集素,大豆丛枝菌根真菌侵染结构的荧光标记活性染色法,可以清晰地检测到大豆丛枝菌根中所有的共生结构,包括丛枝,泡囊和根内菌丝等。2)在丛枝菌根真菌侵染的根中,各种共生结构都呈现出黄色荧光,为DAPI与多聚磷结合在紫外光激发下的呈色。根段中部分细胞内的蓝白色斑点为DAPI与细胞核中DNA结合的显色结果。在含有成熟丛枝结构的细胞中,也可观察到大部分丛枝呈蓝白色,主要是丛枝膜质结构的呈色。因此,利用荧光染料4’,6-二脒基-2-苯基吲哚二盐酸盐染色法定位多聚磷,能很好地区分多聚磷酸盐、DNA和膜质。3)在以上研究的基础上,通过荧光光路的切换,可以同时观察到菌根共生结构和多聚磷的共定位。处于发育阶段的整个丛枝中多聚磷累积的亮黄色清晰可见。在成熟的丛枝中,由于膜质结构发达,对累积在丛枝结构中的多聚磷的染色观察产生了一定影响,导致仅仅局部的多聚磷累积清晰可见。【结论】本研究建立的大豆菌根共生结构与多聚磷累积的双定位分析系统,能够直观观察植物与丛枝菌根真菌的养分交换,清晰地对丛枝菌根共生结构中多聚磷的累积进行定位分析,可作为从组织和细胞水平研究菌根共生体的重要技术手段。 相似文献