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<正>血凝(HA)和血凝抑制(HI)试验是利用某些病毒具有凝集红细胞的特性,且这种特性能被特异性抗血清抑制的原理,用于已知病毒检查血清中相应抗体状态和已知血清鉴定未知病毒的一种兽医实验室最常用的实验方法。 相似文献
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1血凝实验(HA)、血凝抑制实验(HI)简介一些禽病病毒如新城疫病毒、流感病毒、传染性支气管炎病毒(经浓缩和酶处理后)、腺病毒127株及几种支原体具有凝集红细胞的活性(血凝素-神经酸酶HN蛋白与红细胞表面受体结合)。这种红细胞凝集现象可被特异性免疫血清所抑制,即血凝抑制实验(HI)。用该实验可检测鸡体注苗后抗体应答水平及证实是否曾受感染。HI实验的基本成分是血凝(HA)抗原,系列稀释的血清和红细胞悬液(0·5%~1·0%)。实验可在小试管或微量板(96孔V型板)中进行。生产中一般多用固定抗原稀释血清法(β-法),而很少用固定血清稀释抗原… 相似文献
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为观察猪流行性腹泻病毒(PDEV)的血凝特性,进而为建立PEDVHA和HI试验,评估疫苗免疫抗体水平奠定基础,将CV777、DR13、YN13和YN144共4株PEDV毒株,在37℃下,分别用0、5、10、50、100μg/mL胰蛋白酶处理30min,然后用鸡、兔和猪红细胞进行血凝(HA)试验;用已经鉴定为PEDV抗体阳性的血清和乳清分别进行血凝抑制(HI)试验。结果显示:4株PEDV毒株只与兔红细胞发生凝集反应;CV777、DR13毒株的HA现象需要用5μg/mL的胰蛋白酶进行预处理,但是YN13和YN144毒株的HA现象不需要胰蛋白酶作用;该凝集现象能够被PEDV抗体特异性抑制。结果表明,PEDV能够凝集兔的红细胞,不能凝集鸡和猪的红细胞,因而可以用兔红细胞建立PEDV的HA和HI试验方法。 相似文献
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水禽新城疫血凝抑制试验存在的问题 总被引：3，自引：0，他引：3
新城疫病毒能够与鸡红细胞发生凝集现象,这种红细胞凝集现象可被特异性免疫血清所抑制,即红细胞凝集抑制试验(HI)。由于血凝反应可被特异性抗体所抑制,抗体与病毒结合后,血凝素不能吸附于红细胞表面的受体上,出现凝集抑制现象。该方法具有以下优点:①敏感性强,可以检测到微量的抗体,结果也较为准确,是较敏感的血清学反应之一;②特异性强,病毒凝集红细胞只能被特异的抗体所抑制;③检测速度快,1次HI试验只需2 h左右,即可判定结果;④HI试验对环境要求不高,操作简单,1次还可检测大量的样品。因此血凝抑制试验用于水禽血清,可以检测血清中的抗… 相似文献
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血凝试验（HA）和血凝抑制试验（HI）是目前兽医实验室常用的检测方法，作为血清诊断技术之一，是利用某些病毒具有凝集红细胞的特性，且这种特性能被特异性抗血清抑制的原理，用于已知病毒检查血清中相应抗体状态和已知血清鉴定未知病毒的一种兽医实验室最常用的实验方法。HA和HI试验具有特异性好、操作方便、可同时进行大量样本试验、对试验环境要求不高等优点， 相似文献
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《中国预防兽医学报》2015,(8)
为建立H3N2亚型犬流感病毒(CIV)血清学检测方法,本研究利用H3N2亚型CIV重组核蛋白(r NP)作为检测抗原,通过优化反应条件,建立了检测CIV核蛋白血清抗体的间接ELISA方法。通过检测10份SPF犬血清样品确定阴阳性临界值为0.288。该方法检测犬瘟热病毒、犬细小病毒、犬副流感毒、犬腺病毒Ⅱ型、狂犬病病毒、犬弓形虫、犬弓首蛔虫、犬复孔绦虫的阳性血清均为阴性,具有良好的特异性,但与H1N1、H3N8和H9N2亚型CIV有交叉反应。该方法检测H3N2 CIV血清抗体的灵敏度为血凝抑制试验(HI)的3~12.5倍;而且其批内批间变异系数为1.85%~6.57%,重复性良好。通过对H3N2 CIV攻毒犬血清进行分析,表明该检测方法具有滞后性,检测到CIV抗体的时间晚于HI试验。利用本研究建立的方法和以H3N2 CIV为诊断抗原的HI检测方法对450份血清样品进行检测,结果显示该方法对血清样品具有初筛作用,两者阳性符合率为58.3%,阴性符合率为100%。本研究可以结合其它血清学方法为CIV流行病学调查进行快速、高效的检测。 相似文献
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禽流感病毒能够与鸡红细胞发生凝集现象，这种红细胞凝集现象可被特异性免疫血清所抑制，即红细胞凝集抑制实验(HI)。由于血凝反应可被特异性抗体所抑制，抗体与病毒结合后，血凝素不能吸附于红细胞表面的受体上，因此血凝抑制试验可以用于：①检涮血清中的抗体水平；②鉴定病毒；③辅助诊断病毒性疾病；④作为适时免疫的辅助手段， 相似文献
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J Tessler 《Canadian journal of veterinary research》1985,49(1):109-111
A comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in Vero cells with and without cortisone plus cytochalasin B. The titer of NJ-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin B and cortisone. The SCT and CDC strains had approximately the same titers in the egg titration and the fluorescent antibody assay for samples with and without the antimetabolites. 相似文献
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家禽饲料代谢能的评定技术 总被引：1，自引：0，他引：1
家禽饲料代谢能的评定是家禽饲料营养价值评定中一个极其重要的内容，是研究家禽营养需要量的基础，也是配制最佳饲料配方的关键，对提高家禽的生产效率、节约饲料资源具有重要意义。因此，探索家禽饲料代谢能的快速、简便、准确的评定方法一直是动物营养研究中的热点。世界各国的营养学家们经过几十年的探索和努力，摸索出了许多科学、实用的代谢能测定方法，将这些方法归类主要有常规法、快速测定法和体外法等。 相似文献
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Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.Key words: bovine rotavirus, plaque assay, trypsin, diethyl-aminoethy1-dextran 相似文献
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Validation of a von Willebrand factor antigen enzyme-linked immunosorbent assay and newly developed collagen-binding assay 
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下载免费PDF全文 No single test is comprehensive enough to detect all of the variants of von Willebrand Disease (VWD), making determination of both concentration and function of von Willebrand Factor (VWF) important for an accurate diagnosis. The objective of the study was to validate a newly developed VWF collagen binding assay (VWF:CB) and VWF antigen enzyme-linked immunosorbent assay (ELISA) developed at the Ontario Veterinary College (OVC VWF:Ag). Linearity, sensitivity, and coefficients of variation were determined. The Asserachrom VWF:Ag ELISA was used as the reference assay for this study. Concordance correlation and Bland-Altman plots were used to evaluate agreement between both VWF:Ag assays. The VWF:CB accuracy was assessed by degree of association with the VWF:Ag assays, and the VWF:Ag to VWF:CB ratio. All assays were assessed for their ability to distinguish between VWD negative and VWD positive patients. Linearity, intra-assay coefficients of variation, and inter-assay coefficients of variation were acceptable for both the newly developed VWF:CB (R2 = 0.97, average CV = 4.4, and 15, respectively) and OVC VWF:Ag assays (R2 = 0.96, average CV = 7.9, and 5.9, respectively). Agreement between the OVC VWF:Ag assay and reference assay was excellent (ρc = 0.89), and although differences between assay results precluded interchangeable use of the assays, both successfully distinguished VWD positive and VWD negative dogs (P < 0.0001). The VWF:CB showed a strong association with both VWF:Ag assays (R2 = 0.86, 0.82) and VWF:Ag to VWF:CB ratios (≤ 1) were as expected. The excellent performance of both assays in this validation study confirm their reliability and potential for clinical application. 相似文献
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目的比较宽点酶标法与癍点金标免疫渗滤法检测牛血吸虫病的效果。方法选择疫区水牛，耳静脉取血制备血纸，作为待检样品。分别采用斑点金标免疫渗滤法与癍点酶标法检测，比较检测结果。结果癍点金标渗滤法检测仅需30min，而斑点酶标法则需160min。结论疵点金标免疫渗滤法检测牛血吸虫病，操作简便易行、快捷，省时、省工，工作效率成倍提高。 相似文献
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Comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. 总被引：2，自引：0，他引：2 下载免费PDF全文
下载免费PDF全文 A rapid, simple and inexpensive dot immunobinding assay (DIA) was evaluated for the serodiagnosis of paratuberculosis in cattle. The assay was performed on nitrocellulose strips which were dotted with purified protoplasmic antigen of Mycobacterium paratuberculosis. After incubation with test serum samples, the bound antibodies were detected using an enzyme-amplified immunostaining procedure. The efficacy of DIA as a screening test for paratuberculosis was compared to that of an enzyme-linked immunosorbent assay (ELISA), a modified agar gel immunodiffusion (mAGID) test, and an AGID test using 329 serum samples from cattle which were examined for M. paratuberculosis infection by a sensitive fecal culture technique. The DIA and ELISA had comparable results and both of the enzyme immunoassays had higher sensitivity than tests based on AGID. The sensitivity of all four tests was influenced by the intensity of fecal bacterial shedding. Preabsorption of sera with Mycobacterium phlei increased the sensitivity of both enzyme immunoassays. the specificity but reduced the sensitivity of both enzyme immunoassays. 相似文献