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1.
Monitoring of persistent QPX infections in clams of Raritan Bay (New York) shows certain areas of the estuary have remained without any significant disease prevalence. This study was conducted to investigate the potential to mitigate QPX disease by relocating infected hard clams, Mercenaria mercenaria (Linnaeus), from enzootic areas to nearby sites with prevailing environmental conditions suggested to deter infection and favour remission and healing. Clams were collected from a location with consistent disease prevalence in central Raritan Bay and brought to near shore habitats subject to lower salinities and higher summer temperatures. A reduced host density treatment was included in the study to examine the common observation of high clam density in the most persistently infected locales. An additional treatment retained clams above the sediment, since sediments are suspected to represent a QPX reservoir. At the end of the 4‐month study all treatments displayed less QPX disease than the control group and the greatest contrast was provided by the disappearance of infections in a tidal creek.  相似文献   

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3.
This study characterises Perkinsus atlanticus disease prevalence and intensity within the economically important beds of the clam Ruditapes decussatus along the Iberian Atlantic coast. Samples were collected from five different sites along the Portuguese coast (Ria Formosa, Ria do Alvor, V.N. Milfontes, Aveiro, Lagoa de Obidos) and in Galicia (Ria de Arosa) between winter 2000/2001 and 2002/2003. The infection level was evaluated by the Ray Fluid Thioglycollate medium (RFTM) method using the body burden assay. In addition, the measure of the condition index (CI) (percentage between the edible part and the total weight of the clams) was conducted to investigate a possible correlation between this parameter and the intensity of the infection. However, no clear relationship between infection intensity and condition index (CI) could be determined although a significant decline in CI was found for heavily infected clams. Results of a 2-year survey demonstrate the presence of Perkinsus sp. in all sampling sites albeit with different intensities. Sites where human interference was minimal showed the lowest levels of infection. There were no significant differences in Perkinsus sp. infection intensity between samples collected in winter and summer, in contrast with several studies describing higher intensities at the end of the summer. Nevertheless, major differences were observed from year to year and site to site, indicating that factors other than those responsible for seasonal climatic variations might affect the prevalence and the intensity of Perkinsus sp. infection.  相似文献   

4.
Wild bluegill, Lepomis macrochirus Rafinesque, succumb to seasonal mortality in the early spring during cool water temperatures, shown previously to be related to bacteraemia caused by a psychrotrophic bacterium, Pseudomonas mandelii. In the study herein, intestinal coccidiosis in wild bluegill had seasonal prevalence causing heavy intestinal infections and sloughing of intestinal epithelium occurring in late winter/early spring. Infections were predominantly related to two different species, Goussia washuti n. sp., an epicellular coccidium, and a coccidium closely resembling Goussia desseri Molnár 1996, previously only described in percid fish in Europe. In 2019, co‐infections of bacteraemia and intestinal coccidiosis occurred in bluegills. Evaluating coccidium infection intensity by fresh parasite examination and histology, an association was observed in which fish with moderate‐to‐heavy intestinal coccidiosis were 8–12 times more likely to have bacteraemia compared to fish with no or light coccidiosis. The association of these co‐infections suggests that intestinal coccidiosis could contribute to seasonal bacterial epizootics of wild bluegill.  相似文献   

5.
Abundance, length‐frequencies and distribution of Ruditapes philippinarum (Manila Clams) and Tellina (Quidnipagus) palatum were measured at two beaches in Kāne'ohe Bay, O'ahu, Hawai'i in June, 2010. Abundances had decreased from 866.2 m?2 to 3.4 m?2 for Ruditapes and from 75.5 m?2 to 1.5 m?2 for T. palatum since 1977. Distribution of both species was patchy, but both were most commonly found >40 m from shore. Size frequencies of live clams compared with empty shells suggest that few Ruditapes survive to sexual maturity. A similar trend was not detected for T. palatum. Aquaculture trials of R. philippinarum were conducted at the He'eia and Moli'i traditional Hawaiian fishponds in the same bay. The clams failed to thrive, although triploid and diploid Crassostrea gigas performed well in concurrent trials in the same fishponds. Current lower abundances for wild R. philippinarum could be due to factors related to predation or nutrient limitations. Previously, two large sewage outfalls existed at the surveyed clam bed areas which may have temporarily increased nutrient availability for both wild and cultured clams. Current nutrient levels may inhibit Manila clam growth and recruitment in Kāne'ohe Bay.  相似文献   

6.
Haemic neoplasia was first considered a disease of concern for soft‐shell clams in Prince Edward Island (PEI) when it was diagnosed as the cause of mass mortalities in 1999. The aetiology of the disease remains elusive, but has been associated with environmental degradation. In this study, a 2‐year (2001–2002) geographic and seasonal survey was conducted for haemic neoplasia, using histology, in soft‐shell clams from PEI. In addition, using geographic information system, the association between anthropogenic factors in the watersheds at sites affected by haemic neoplasia and the prevalence of the disease was investigated. Finally, histopathological changes were assessed in soft‐shell clams experimentally exposed to four concentrations of chlorothalonil for 27 days. Haemic neoplasia could not be induced at any concentration of chlorothalonil. Clams exposed to a concentration of 1000 μg L?1 of the fungicide, however, exhibited an LC50 of 17 days. Although this information provides additional toxicity information (LC50) for soft‐shell clams, further experiments are required to assess longer term exposure to the fungicide. The highest prevalences of haemic neoplasia in PEI were found in North River and Miscouche (28.3–50.9% and 33.0–77.8%, respectively). No clear seasonal patterns were found. There was a correlation between haemic neoplasia prevalence and watersheds with a high percentage of potato acreage and forest coverage (= 0.026 and = 0.045, respectively), suggesting a link between anthropogenic activity and the prevalence of the disease.  相似文献   

7.
Epinephelus is among the most important fish genera in the Southeast Asian Pacific coastal systems. In Chinese coast, the orange‐spotted grouper Epinephelus coioides is one of the most significant species based on culture volume, and it is consumed all over the world. Very little information exists on this species’ parasitofauna. Composition and temporal variation in the community structure of the gill monogeneans on wild versus cultured E. coioides from Daya Bay (23°25′N, 117°2′E), South China Sea were determined using seasonal samples taken between April 2008 and January 2009. Eight species of monogeneans of three families and four genera on the gills of E. coioides were found: Neobenedenia melleni, Haliotrema cromileptis, Diplectanum grouperi, Pseudorhabdosynochus justinei, P. lantauensis, P. coioidesis, P. serrani and P. shenzhenensis. Eight of the monogenean species exhibited seasonal variation in their infection dynamics associated with environmental changes during seasons. The variations in the infection dynamics generated changes in the community structure during the sampling periods. Moreover, in the three different host living environments (wild, monocultured and polycultured), the monogenean communities exhibited the different seasonal patterns.  相似文献   

8.
The sea louse, Lepeophtheirus salmonis (Krøyer 1837), is a significant parasite of farmed salmon throughout the Northern Hemisphere. Management of on‐farm louse populations can be improved by understanding the role that wild fish play in sustaining and providing refuge for the local population of sea lice. In this study, 1,064 sticklebacks were captured. Of these animals, 176 individuals were carrying a total of 238 sea lice, yielding a prevalence and intensity of 16.5% and 1.4 lice per fish, respectively. Detailed examination of the sea lice on the three‐spined sticklebacks captured in Cobscook Bay found two L. salmonis individuals using three‐spined sticklebacks as hosts. A 2012 survey of wild fish in Cobscook Bay, Maine, found multiple wild hosts for Caligus elongatus (von Nordmann 1832), including three‐spined sticklebacks (Gasterosteus aculeatus L.), but no L. salmonis were found in this earlier study.  相似文献   

9.
Growth, intensity of Perkinsus marinus (Levine) infection, and survival of synchronously spawned North Carolina (NC) and Chesapeake Bay‐heritage (CB) oysters, Crassostrea virginica (Gmelin) were evaluated under standard tray culture conditions at several sites in both regions (Wye River, Maryland; Mobjack Bay, Virginia; Pamlico River, NC and Bogue Banks, NC). Infection prevalence reached 100% in oysters held at all high‐ and moderate‐salinity sites, at which time the CB strain ceased to grow. Shortly after growth ceased, CB oysters exhibited mortality that rapidly progressed to 100%. Unlike the CB strain, growth continued in the NC strain despite high P. marinus prevalence. When mortality did occur in the NC strain, at a reduced rate of 37–40%, it was associated with higher intensity of P. marinus than the infection intensity correlated with death of CB oysters. At the low‐salinity site in NC, P. marinus infection persisted at low weighted prevalence throughout the latter portion of the culture period but was not associated with mortality of either strain. These trends in growth and disease resistance for the two strains demonstrate that aquaculture performance is related to the level of disease resistance in oyster strains, salinity of water in growing areas and virulence of P. marinus.  相似文献   

10.
The bacterium Edwardsiella ictaluri is considered to be one of the most significant pathogens of farmed catfish in the United States of America and has also caused mortalities in farmed and wild fishes in many other parts of the world. E. ictaluri is not believed to be present in wild fish populations in Australia, although it has previously been detected in imported ornamental fishes held in quarantine facilities. In an attempt to confirm freedom from the bacterium in Australian native fishes, we undertook a risk‐based survey of wild catfishes from 15 sites across northern Australia. E. ictaluri was detected by selective culturing, followed by DNA testing, in Wet Tropics tandan (Tandanus tropicanus) from the Tully River, at a prevalence of 0.40 (95% CI 0.21–0.61). The bacterium was not found in fishes sampled from any of the other 14 sites. This is the first report of E. ictaluri in wild fishes in Australia.  相似文献   

11.
In situ controlled systems based on PVC cages surrounded by a net mesh and buried in sand were designed to determine the causes of important mortalities in producing locations of carpet shell clam, Ruditapes decussatus, in two important production areas of Galicia (NW Spain). The pathogenic status of the clam populations (focusing mainly on Perkinsus sp. and clam haplosporidian) as well as changes in water temperature and salinity were recorded. Sampling was conducted during a short period of time when high mortalities were present in the two locations and in a 1‐year study when no important mortalities were registered. We found that the environmental conditions and not the presence of pathogens seemed to be related to mortalities in the study. Our results suggest that mortality rates, especially in natural beds, can emerge from a complex synergy of different factors. Moreover, we propose in situ controlled systems based on PVC cages as a useful method to control infaunal bivalve mortalities in natural beds.  相似文献   

12.
Environmental variation can shape phenotypic variation in organisms, but most evidence for trait differentiation comes from analyses of dichotomous habitat types that differ in only one or few key environmental factors. In reality, environmental variation is often more subtle, gradual and multifarious. Here, we investigated geographic variation in body shape of two darter species (Etheostoma spectabile and Etheostoma flabellare; Percidae) that occur along river gradients. This study addressed three specific questions: Is there intraspecific geographic variation in the two species across different sites in the Ozark Highlands of Oklahoma (USA)? Is phenotypic variation across sites correlated with abiotic environmental conditions? Do the two species share site‐specific (i.e. convergent) phenotypic variation in areas where they occur together? Our results indicated significant body shape variation in both species. Population differences in body shape were particularly correlated with variation in substrate composition. The combined analysis of both species indicated a small but significant effect of convergence on body shape wherever they are sympatric; shared variation, however, was not related to any environmental variables included in the analysis. While it remains unclear whether phenotypic variation in these species is due to heritable differentiation or environmentally induced plasticity, our results indicate that even subtle and gradual environmental variation can induce substantial variation in phenotypes on a relatively small spatial scale.  相似文献   

13.
Currently, Tapes philippinarum, the Manila clam, is the primary fishery resource for about 1,000 fishermen and aquaculturists in the Venice Lagoon. The recruitment of this clam is a key component supporting shellfish culture industry that is almost completely dependent on the gathering of wild clam seed in nursery areas where the juveniles accrue at high densities as a consequence of favourable environmental conditions. The objective of the present study was to identify the areas optimal for the recruitment of T. philippinarum, herein referred to as nursery areas, and to evaluate the recruitment success each year for the period 2002–2007. Sampling was conducted with a hydraulic dredge at 25 stations, in at least three separate periods from April to September each year for 2002, 2003 and 2004, and at 15 stations for 2005, 2006 and 2007. The zero-year cohorts of this species were usually first detected in bottom samples in late spring (May and June). Some setting appears to occur throughout the summer. The peak of seed density, expressed as number of individuals retained on a 1-mm mesh screen per square metre of sampled substrate, was recorded in September. Measurable fluctuations in annual recruitment have been observed, with peak densities of juveniles in 2002 and 2003 (about 2,000/m2) and minimum densities in 2006 (about 44/m2). In the summer 2007, densities over 30,000/m2 were found at the mouth of the River Brenta, immediately adjacent to the southern end of the Venice Lagoon. These intensive, multiyear surveys of this species furnish critical information on seasonal reproduction patterns and recruitment success, which is essential for the rational development of a local fishing master plan and for the formulation of a management strategy for sustainable utilisation of this shellfish resource in the Venice Lagoon.  相似文献   

14.
This study presents an Ex‐Post Facto analysis of diseases of wild juvenile and adults of Litopenaeus setiferus collected from a field survey at the Natural Protected Area of Terminos lagoon, southern Mexico. The objective of the present approach was to determine if sampling site and/or shrimp age were contributing risk factors for disease between juvenile and adult shrimp; if there was a determined period of time in a year cycle when diseases were more critical, and if the analysis would help to decision‐ making considering what population would pose less risk of disease‐carrying when withdrawn for experimental purposes; all under an after‐the‐fact (ex‐post facto) approach. We identified that juvenile shrimp were at more risk of contracting some diseases in the estuarine environment and June, July and August months, were found to be a critical period when colonizing and parasitic diseases maintained a significant high prevalence in the shrimp population. These assumptions may help for decision‐making when wild shrimp have to be withdrawn from their natural environment for research purposes.  相似文献   

15.
Proliferative kidney disease (PKD) of salmonids caused by Tetracapsuloides bryosalmonae causes high mortalities of wild brown trout (Salmo trutta fario) and farmed rainbow trout (Oncorhynchus mykiss) at elevated water temperatures. Here the aim was to compare the temperature‐dependent modulation of T. bryosalmonae in the two salmonid host species, which display different temperature optima. We used a novel experimental set‐up in which we exposed brown trout and rainbow trout to an identical quantified low concentration of T. bryosalmonae for a short time period (1 hr). We followed the development of the parasite in the fish hosts for 70 days. PKD prevalence and parasite kinetics were assessed using qPCR. Exposures were performed at temperatures (12°C and 15°C) that reflect an environmental scenario that may occur in the natural habitat of salmonids. T. bryosalmonae infection was confirmed earliest in brown trout kept at 15°C (day 7 post‐exposure) while, in all other groups, T. bryosalmonae was not confirmed until day 15 post‐exposure. Moreover, significantly greater infection prevalence and a faster increase of parasite intensity were observed in brown trout kept at 15°C than in all other groups. These results indicate that PKD is differentially modulated by water temperature in related host species.  相似文献   

16.
The pathways by which pathogens invade Fenneropenaeus indicus, the potential colonization in various tissues and the disease transmission mechanisms are unclear. The aims of the present study were to visualize the colonization and pathogenesis of GFP‐tagged Vibrio parahaemolyticus, in various tissues of F. indicus to evaluate the pathogen interaction. Among the three strains isolated, a virulent strain VpDAHV2 was tagged with green fluorescent protein (GFP‐VpDAHV2) and validated for both its growth characteristics and its virulence as a genuine model for F. indicus infection. VpDAHV2 was positive for toxR and tlh genes and negative for tdh genes. CLSM images revealed that maximum colonization was observed in the haemolymph of the F. indicus challenged with GFP‐VpDAHV2. The haemolymph was the primary site for the colonization of GFP‐VpDAHV2 in F. indicus. The enteric localization occurred independently of the flagellum or motility of GFP‐VpDAHV2 through the intestinal route. The F. indicus infection model suggests that the haemolymph and the intestine represent the sites of infection by GFP‐VpDAHV2, and hence are the active sites of pathogen interactions. GFP tagging of V. parahaemolyticus is a new and systemic approach to determine the presence of bacteria in vivo for the confirmation of host pathogen interactions in aquaculture studies.  相似文献   

17.
《水生生物资源》2002,15(2):129-137
The abundance of prey ingested by brown trout (Salmo trutta) were compared with the abundance of drifting invertebrates in a mountain stream managed by hydropeaking, upstream (site A) and downstream (site B) of a hydroelectric power plant. During power generation, flow and temperature were the two main environmental factors modified. The natural flow in the river below the outlet may be enhanced several times a day from 1 to 11 m3.s–1 in summer and winter, and from 5 to 15 m3.s–1 during spring spates. During hydropeaking, the water was cooled by an average of 6 °C in summer and warmed by an average of 2 °C in winter. Overall drift density was higher at site B than at site A. There was a clear diel pattern of drift at site A, with low drift density during the day and high drift density at night, whereas no clear diel pattern was observed at site B. Below the plant, at site B, drift pattern appeared to be influenced most by hydropeaking. The flushing action of peaking flows caused a catastrophic drift, which was highest in autumn when the difference between natural and peak flows was greatest. Juvenile trout were adversely affected by hydropeaking conditions and subsequently their density and biomass were reduced by 30% from site A to site B, whereas no significant difference was noticed for adults. Gut contents analysis showed that brown trout chiefly fed on the most available prey items at both sites. Fish did not seem to feed in response to diel drift patterns above the plant, whereas they chiefly used drift pulses generated by peaking flows below the outlet. Under natural conditions, fullness indices increased from autumn to summer, suggesting they may be related to prey availability and changes in water temperature. In the regulated section, fullness indices were the lowest in spring, i.e. the season when peak flows added to snowmelt floods, suggesting a prominent role of high current velocities through habitat suitability, position maintenance, and ability to capture preys. Although hydropeaking is known to disturb trout population dynamics in this and other rivers, this kind of river regulation (natural discharge except during periods of power generation, and intermittent hydropeaking from a separate reservoir) allowed the maintain of brown trout below the outlet, probably because the river returned to natural conditions when the plant was inoperative, and because daily artificial fluctuations in flow and temperature remained within the limits of natural seasonal variations.  相似文献   

18.
Amoebic gill disease (AGD) caused by the amoeba Paramoeba perurans is an increasing problem in Atlantic salmon aquaculture. In the present PCR survey, the focus was to identify reservoir species or environmental samples where P. perurans could be present throughout the year, regardless of the infection status in farmed Atlantic salmon. A total of 1200 samples were collected at or in the proximity to farming sites with AGD, or with history of AGD, and analysed for the presence of P. perurans. No results supported biofouling organisms, salmon lice, biofilm or sediment to maintain P. perurans. However, during clinical AGD in Atlantic salmon, the amoeba were detected in several samples, including water, biofilm, plankton, several filter feeders and wild fish. It is likely that some of these samples were positive as a result of the continuous exposure through water. Positive wild fish may contribute to the spread of P. perurans. Cleaner fish tested positive for P. perurans when salmon tested negative, indicating that they may withhold the amoeba longer than salmon. The results demonstrate the high infection pressure produced from an AGD‐afflicted Atlantic salmon population and thus the importance of early intervention to reduce infection pressure and horizontal spread of P. perurans within farms.  相似文献   

19.
A survey on the presence of the viruses of two economically significant diseases, white spot syndrome virus (WSSV) and monodon baculovirus (MBV) in wild‐collected Penaeus monodon broodstock, was conducted during different seasons of the year in two major coastal areas of southeast India. The broodstock were collected along the coast of Tamil Nadu and Andhra Pradesh during summer, premonsoon, monsoon and post‐monsoon seasons for three consecutive years. A total of 7905 samples were collected and subjected to MBV screening, and 6709 samples that were screened as MBV negative were diagnosed for WSSV. MBV was detected using rapid malachite green staining and WSSV by nested polymerase chain reaction. Prevalence data of the viruses were analysed using the EpiCalc 2000 program at 95% confidence interval. Samples collected from the Andhra Pradesh coast displayed a slightly higher prevalence of WSSV and MBV infection than those collected from Tamil Nadu, although this difference was not statistically significant (P > 005). In addition, it was found that the prevalence of both WSSV and MBV infections fluctuated according to season. Data on prevalence of these viruses in broodstock would be useful to develop strategies for shrimp health management along the southeast coast of India.  相似文献   

20.
An epidemiological study was carried out in Norway in 2015–2018, investigating the development of infection with Piscine myocarditis virus (PMCV) and development of cardiomyopathy syndrome (CMS) in farmed Atlantic salmon. Cohorts from 12 sites were followed and sampled every month or every other month from sea transfer to slaughter. PMCV was detected at all sites and in all sampled cages, and fish in six sites developed clinical CMS. The initial infection happened between 1 and 7 months post‐sea transfer, and the median time from infection with PMCV until outbreak of CMS was 6.5 months. Generally, fish from sites with CMS had higher viral titre and a higher prevalence of PMCV, compared to sites that did not develop clinical CMS. The virus persisted until the point of slaughter at most (11 out of 12) of the sites. The detection of PMCV in all sites suggests that PMCV is more widespread than previously known. Screening for PMCV as a tool to monitor impending outbreaks of CMS must be supported by observations of the health status of the fish and risk factors for development of disease.  相似文献   

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