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1.
Bioethanol production in North America has led to the production of considerable quantities of different co‐products. Variation in nutrient profiles as well as nutrient availability among these co‐products may lead to the formulation of imbalanced diets that may adversely affect animal performance. This study aimed to compare three types of dried distiller’s grains with solubles [100% wheat DDGS (WDDGS); DDGS blend1 (BDDGS1, corn to wheat ratio 30:70); DDGS blend2 (BDDGS2, corn to wheat ratio 50:50)] and their different batches within DDGS type with regard to: (i) protein and carbohydrate sub‐fractions based on Cornell Net Carbohydrate and Protein System (CNCPS); (ii) calculated energy values; and (iii) rumen degradation of dry matter (DDM), organic matter (DOM), crude protein (DCP), neutral detergent fibre (DNDF) and starch (Dstarch) at 36 and 72 h of ruminal incubations. Wheat DDGS had a lower intermediately (PB2, 136.4 vs. 187.4 g/kg DM) and a higher slowly degradable true protein (PB3, 142.2 vs.105.3 g/kg DM) than BDDGS1, but similar to those of BDDGS2. Sugar (CA4) was higher, whereas starch (PB1) and digestible fibre (PB3) were lower in WDDGS than in BDDGS1 and BDDGS2. All carbohydrate sub‐fractions determined differed significantly between the two batches of BDDGS2. The BDDGS2 had the highest calculated energy values (TDN, DE3×, ME3×, NEL3×, NEm and NEg) among the three DDGS types. The energy values were slightly different between the batches of the three DDGS types. At all incubation times, wheat DDGS had a significantly higher (p < 0.05) DDM, DOM, DCP and DNDF than both DDGS blends. Differences were observed between different batches within DDGS types with regard to in situ rumen degradation of DM, OM, CP, NDF and starch. In conclusion, differences were observed in protein and carbohydrate sub‐fractions and in situ ruminal degradation of DM, OM, CP, NDF and starch among the three DDGS types and different batches within DDGS type. This indicates that the nutrients supplied to ruminants may not only differ among different types of DDGS but it may also differ among different batches within DDGS type.  相似文献   

2.
为研究日粮粗蛋白质水平(crude protein,CP)对育肥湖羊瘤胃微生物、pH及VFA含量的影响,选取54只4月龄[(32.25±2.50)kg]湖羊公羔,采用完全随机分组设计,将试验羊分为3个处理组,每个处理组18个重复,每个重复1只羊,3个处理组日粮CP水平分别为11.5%、14.0%、16.5%,试验期共70 d,包括7 d预试期,63 d正试期。试验期结束后,每组选择10只试验羊进行屠宰,测定屠宰性能并采集瘤胃内容物,用于瘤胃微生物DNA提取。结果表明:1)不同CP处理组对宰前活重、胴体重、屠宰率、瘤胃总重、瘤胃组织重及尾脂重无显著影响(P>0.05)。2)11.5%CP组瘤胃液氨态氮显著低于14.0%CP及16.5%CP组(P<0.001),但14.0%CP组与16.5%CP组无显著差异(P>0.05)。瘤胃液pH随日粮蛋白水平的增加有增加的趋势(P<0.1)。3)总挥发性脂肪酸(total volatile fatty acids,TVFA)浓度不受日粮中CP水平的影响(P>0.05),其中乙酸、丙酸、异丁酸、丁酸、戊酸比例均不受CP水平的影响(P>0.05),但异戊酸的比例随日粮中CP水平的增加有增加趋势(P=0.098)。4)在不同CP处理组中,瘤胃内溶纤维丁酸弧菌含量最高,但各处理组间无显著差异(P>0.05),其中白色瘤胃球菌、产琥珀酸丝状杆菌、普雷沃氏菌的含量均不受CP水平的影响(P>0.05),而黄色瘤胃球菌11.5%CP组显著低于14.0%CP组(P<0.05),16.5%CP组与11.5%CP组及14.0%CP组无显著差异。综上所述,日粮不同CP水平不影响试验羊屠宰性能,但能够影响瘤胃液pH及氨态氮含量,随CP水平的增加,异戊酸的比例有增加的趋势,14.0%CP组黄色瘤胃球菌数最多。  相似文献   

3.
本试验旨在研究低蛋白质氨基酸平衡饲粮对黄羽肉种鸡生产性能、蛋品质、孵化性能和氮排放的影响。选用30周龄健康的快大型岭南黄羽肉种母鸡288只,根据体重和产蛋率相近原则,随机分为4个组,每组6个重复,每个重复12只鸡。各组饲粮粗蛋白质(CP)水平分别为16%(对照组)、15%、14%、13%,各组的赖氨酸、蛋氨酸、色氨酸、苏氨酸、缬氨酸、亮氨酸及异亮氨酸水平一致。试验期10周。结果表明:1)低蛋白质氨基酸平衡饲粮对黄羽肉种鸡末重、平均日增重、平均日产蛋量、料蛋比、平均蛋重、产蛋率、破蛋率和不合格蛋率均无显著影响(P>0.05)。2)低蛋白质氨基酸平衡饲粮对黄羽肉种鸡鸡蛋的蛋形指数、蛋白高度、哈氏单位、蛋黄重、蛋壳重和蛋壳厚度均无显著影响(P>0.05)。13%CP组的蛋黄颜色显著高于16%CP组(P<0.05)。3)低蛋白质氨基酸平衡饲粮对黄羽肉种鸡的雏鸡初生重、受精蛋孵化率、弱雏率和受精率均无显著影响(P>0.05)。4)13%、14%CP组的血浆尿素氮含量显著低于16%CP组(P<0.05),13%CP组的血浆尿酸含量显著低于16%CP组(P<0.05),13%CP组的粪便氮含量显著低于16%CP组(P<0.05)。由此可见,低蛋白质氨基酸平衡饲粮对黄羽肉种鸡的生产性能、蛋品质和孵化性能无负面影响,但能够降低血浆尿素氮、尿酸含量和粪便氮含量。本试验条件下,黄羽肉种鸡产蛋高峰期的饲粮CP水平可以降低至13%(补充7种必需氨基酸)。  相似文献   

4.
We evaluated the effect of three sources of dried distillers' grains with solubles (DDGS) in diets of mid‐lactating dairy cows on milk production and milk composition and on digestibility in sheep. DDGS from wheat, corn and barley (DDGS1), wheat and corn (DDGS2) and wheat (DDGS3) were studied and compared with a rapeseed meal (RSM). RSM and DDGS were characterized through in situ crude protein (CP) degradability. Nutrient digestibility was determined in sheep. Twenty‐four multiparous cows were used in a 4 × 4 Latin square design with 28‐day periods. Treatments included total mixed rations containing as primary protein sources RSM (control), DDGS1 (D1), DDGS2 (D2) or DDGS3 (D3). RSM contained less rapidly degradable CP (fraction a), more potentially degradable CP (fraction b) and more rumen undegradable CP (UDP) than the three DDGS. In vivo digestibility of RSM organic matter was similar to DDGS. Calculated net energy for lactation (NEL) was lower for RSM (7.4 MJ/kg DM) than for DDGS, which averaged 7.7 MJ/kg DM. Cows' dry matter intake did not differ between diets (21.7 kg/day). Cows fed D1 yielded more milk than those fed D3 (31.7 vs. 30.4 kg/day); no differences were found between control and DDGS diets (31.3 vs. 31.1 kg/day). Energy‐corrected milk was similar among diets (31.2 kg/day). Diets affected neither milk fat concentration (4.0%) nor milk fat yield (1.24 kg/day). Milk protein yield of control (1.12 kg/day) was significantly higher than D3 (1.06 kg/day) but not different form D1 and D2 (1.08 kg/day each). Feeding DDGS significantly increased milk lactose concentration (4.91%) in relation to control (4.81%). DDGS can be a suitable feed in relation to RSM and can be fed up to 4 kg dry matter per day in rations of dairy cows in mid‐lactation. However, high variation of protein and energy values of DDGS should be considered when included in diets of dairy cows.  相似文献   

5.
高寒草地生物量及牧草养分含量年度动态研究   总被引：2，自引：0，他引：2  
测定了西藏那曲地区高寒草地1~12月份围栏内和围栏外的地上生物量(BM)及牧草粗蛋白质(CP)、粗脂肪(EE)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和灰分(Ash)含量。结果表明:围栏内年平均BM极显著高于围栏外(15.02 DM g/m2对11.70 DM g/m2,P<0.01),而围栏内外牧草各种养分含量差异不显著(P>0.05)。不同季节BM和牧草养分含量变异较大,BM呈“单峰”曲线变化趋势,8月份最高(33.02 DM g/m2),3月份最低(6.39DM g/m2);牧草CP、EE含量亦呈“单峰”曲线变化趋势,CP含量在8月份达到最高(10.68%DM),在2月份降到最低(2.72%DM),EE含量变异不大,在9月份较高(3.77%DM),1月份较低(2.26%DM);NDF变化规律与CP相反,呈“V”型曲线变化趋势,最低和最高含量分别在8月份(49.38%DM)和12月份(69.08%DM);ADF含量全年变化不大,夏季较(35.03%DM)冬春季低(42.92%DM);Ash在6月份含量最高(18.29%DM),2月份含量最低(7.14%DM)。以月份为自变量,进行适当变换后建立了牧草生物量和养分含量的全年动态变化回归模型。  相似文献   

6.
选用平均体重为(40±2)kg杜长大三元杂交去势公猪3头,按3×3的拉丁方进行试验设计,试验分3期,每期为7 d,采用全收粪法,研究不同蛋白质(CP)水平的氨基酸平衡日粮CP水平分别为14%、16%和18%)对氮、磷及能量代谢的影响。结果表明:不同蛋白质水平日粮处理间的氮表观消化率差异不显(著P>0.05),氮的沉积率以14%CP和16%CP组显著高于18%CP组(P<0.05),而14%CP和16%CP组之间无显著差异(P>0.05)。粪氮排出量14%CP日粮组显著低于16%CP和18%CP日粮组(P<0.05),16%CP日粮组和18%CP日粮组间无显著差异(P>0.05)。随着日粮蛋白质水平下降,生长猪的氮排出量、粪氮和尿氮均减少,总排泄氮中尿氮的比例较大,而粪氮则较少。不同蛋白质水平日粮对磷和能量的消化利用无显著影响(P>0.05)。  相似文献   

7.
This study was conducted to determine the compartment 1 (C1) characteristics of alpacas (fistulated male, 7 ± 1.5 years old, 61 ± 5 kg BW) fed grass hay (GH) supplemented with amaranth (AM), quinoa (Q) and barley (B) grains. Alpacas were provided water ad libitum while housed in metabolism crates. The GH and GH plus treatments were fed at 0700 every day. Treatment periods were for 14 days in which GH or GH plus one of the grain treatments were randomly allocated. On day 14, volatile fatty acids (VFA), pH and ammonia nitrogen (NH3‐N) were determined at 1, 3, 6, 10, 14, 18 and 24 h post‐feeding. C1 degradation of each feed component was also determined with the alpacas being fed GH only and the samples incubated for 0, 2, 4, 8, 14, 24, 48 and 72 h. Dry matter (DM), neutral detergent fibre (NDF) and crude protein (CP) were determined and were divided into three categories: a = immediately soluble; b = the non‐soluble but degradable; and u = non‐degradable/unavailable, potential extent of degradation (PE), degradation rate (c) and effective degradation (ED). C1 passage rate was determined using acid detergent insoluble ash as a marker and was calculated to be 5.5%?h‐1. Total DM intake was highest (p < 0.05) for B and resulted in a higher (p < 0.05) CP intake. GH and AM were different in mean pH (6.81 and 6.66, respectively). B NH3‐N was greater (p < 0.05) than the other treatments. Total VFA was greatest (p < 0.05) for AM, with the greatest composition differences being a shift form acetate percentage to butyrate. DM, NDF and CP degradation was different across the treatments, where PE and ED were higher (p < 0.05) for the grain treatments. The pseudo‐grains AM and Q had similar C1 degradation characteristics to B.  相似文献   

8.
The objectives of the present study were to evaluate fasting metabolism (FM) of water buffalo (Bubalus, Bubalis) at three stages of growth (12, 18 and 24 months) in Guangxi, China. Five female water buffalo were used for each age group and their live weight was on average 254, 326 and 338 kg, respectively. All animals were of average body condition, healthy and de‐wormed before start of the study. Prior to a 6‐day fasting period, buffalo were offered a mixed diet of forage and concentrates (70% and 30%, dry matter basis) on a restricted nutritional level (419 kJ/kg0.75 of metabolizable energy, ME) for 15 days. Gas exchanges for each animal were determined for 3 days from day 4 of starvation, using open‐circuit respiratory head hoods. Fasting body weight was 0.918 of live weight (P < 0.001, r2 = 0.99). Both fasting heat production (FHP) and FM (MJ/day) increased significantly with increased age of animals (P < 0.05). Linear regression analysis indicated a positive relationship between fasting body weight (kg0.75) and FHP (MJ/day, P < 0.01, r2 = 0.49) or FM (MJ/day P < 0.01, r2 = 0.52) when using individual animal data across three groups. However, when expressed as kJ/kg0.75 of fasting body weight, the differences in FHP or FM between three groups of animals were not significant. The present average FHP and FM (322 and 347 kJ/kg0.75 of fasting body weight) were compatible to those published in the literature for water buffalo, beef and dairy cattle. The present FM data were also used to estimate net energy (NEm) and ME (MEm) requirements for maintenance for water buffalo. The results for these two parameters were similar to those for FHP and FM. There was no significant difference between three groups of buffalo in NEm or MEm when expressed as kJ/kg0.75 of live weight. The present average NEm and MEm values (347 and 506 kJ/kg0.75 of live weight) are close to those proposed by the Agricultural and Food Research Council adopted in UK for beef and dairy cattle. The results indicate that the present FM data can be used as a basis for rationing water buffalo in China.  相似文献   

9.
The present study investigated the effects of the nutritional levels of diets on meat quality and related gene expression in Hainan black goat. Twenty‐four goats were divided into six dietary treatments and were fed a concentrate‐based diet with two levels of crude protein (CP) (15% or 17%) and three levels of digestive energy (DE) (11.72, 12.55 or 13.39 MJ/kg DM) for 90 days. Goats fed the concentrate‐based diet with 17% CP had significantly (P < 0.05) higher average daily gains (ADG) and better feed conversion rates (FCR). The pH 24h value tended to decrease (P < 0.05) with increasing DE levels. The tenderness of Longissimus dorsi muscle (LD) and Semimembranosus muscle (SM) reduced with increasing CP levels (P < 0.05). With increasing DE levels, tenderness was increased (P < 0.05). The heart fatty acid‐binding protein (H‐FABP) mRNA expression levels in LD and SM increased with increasing DE levels (P < 0.05), but decreased with increasing CP levels (P < 0.05). The calpastatin (CAST) and μ‐calpain mRNA expressions levels in LD and SM were affected significantly (P < 0.05) by CP and DE levels in the diet. Therefore, the nutritional levels of diets affect meat quality and expression levels of genes associated with meat quality in Hainan black goats.  相似文献   

10.
为探索水肥协同作用对不同耕作措施下作物产量影响规律,运用在黄土丘陵区率订和验证后的APSIM(agricultural production system simulator)模型,模拟研究区近35年传统耕作(T)、免耕(NT)和免耕覆盖(NTS)措施下轮作小麦/豌豆产量,并采用多元回归分析施氮量(X1)、休闲期降水量(X2)和生育期降水量(X3)对小麦/豌豆模拟产量的水肥协同效应。结果表明,自然降水条件下3因素对不同耕作措施小麦和豌豆产量的贡献率均为:X3＞X2＞X1。生育期降水量和休闲期降水量对产量的贡献率均为:NTS＞NT＞T。T、NT和NTS措施下小麦和豌豆的产量与施氮量均呈开口向下二次抛物线型变化,但小麦最佳施氮量分别为65.0,65.5和44.5 kg/hm2,豌豆的最佳施氮量分别为17.9,18.5和23.8 kg/hm2,并且施氮量对小麦产量的贡献率为:NT＞T＞NTS,而施氮量对豌豆产量的贡献率为:NTS＞NT＞T。在甘肃省定西黄土丘陵区,决定小麦和豌豆产量的关键因素是降水,降水量对免耕覆盖的增产效应最为显著,且3种耕作措施条件下小麦和豌豆对施氮效应有不同的表现。  相似文献   

11.
A lactation experiment was conducted to determine the influence of addition of pistachio by‐products extract (PBE) to alfalfa silage (AS) on performance, rumen fermentation, milk yield and composition, and microbial nitrogen synthesis. Eight multiparous dairy goats (1.8 ± 0.25 kg of milk yield) were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments to compare two types of AS (supplemented with or without PBE) with two levels of dietary crude protein (14% vs. 16% CP). Dietary treatments were (i) AS with 14% CP of DM diet without PBE (14%CP?PBE), (ii) AS with 14% CP of DM diet with PBE (14%CP + PBE), (iii) AS with 16% CP of DM diet without PBE (16%CP?PBE) and (iv) AS with 16% CP of DM diet with PBE (16%CP + PBE). PBE was sprayed on fresh alfalfa at a ratio of 500 ml/kg alfalfa DM to get the final concentration of 1% tannin as tannic acid equivalent on DM basis. Intake of CP was greater (p < 0.01) in goats fed 16% CP diets than those fed 14% CP diets, regardless of PBE supplementation. Supplementation of PBE tended to decrease (p = 0.09) rumen NH3‐N concentration regardless of the level of CP in the diet. Supplementation of PBE tended (p = 0.09) to decrease total purine derivatives regardless of the level of CP in the diet with no significant change in microbial nitrogen supply. Efficiency of microbial nitrogen synthesis (EMNS) had a tendency (p = 0.07) to decrease in PBE supplemented diets. There was also a tendency (p = 0.10) for more EMNS in 14% CP fed goats than those fed 16% CP diets. Therefore, AS supplemented with PBE may lead to less concentration of ruminal NH3‐N because of decreased degradation of CP by rumen micro‐organisms in response to pistachio by‐products tannins.  相似文献   

12.
不同玉米品种全株青贮品质及营养价值的比较分析   总被引：1，自引：0，他引：1  
试验旨在研究不同品种全株玉米青贮发酵品质和营养价值的差异，选取5个粮饲兼用型玉米品种，于1/2~3/4乳线期刈割，使用聚乙烯发酵袋（50 cm×80 cm）在实验室条件下（15~20℃）发酵60 d，开袋后进行感官评价，测定青贮发酵品质，风干样粉碎后用于常规化学成分及消化率的测定。结果显示：纪元128感官评分等级为中级，其余品种均为良好级。沃锋9的乳酸含量（6.05% DM）显著高于纪元128（5.13% DM）和郑单958（5.13% DM）（P<0.05），乙酸和丙酸含量则极显著低于潞玉36、郑单958和京科25（P<0.01）。5个品种全株玉米青贮V-score评分均>97.00分。京科25的粗蛋白质（CP）、沃锋9的粗脂肪（EE）、郑单958的可溶性碳水化合物（WSC）和纪元128的淀粉含量高于其他品种，分别达9.24% DM、3.11% DM、2.84% DM、30.33% DM。各品种相对饲喂价值（RFV）由高到低依次为：沃锋9（163.00） > 纪元128（160.93） > 郑单958（156.30） > 京科25（152.70） > 潞玉36（151.77）。郑单958和沃锋9的24 h中性洗涤纤维消化率（NDFD）（17.84% DM；18.28% DM）极显著低于京科25和纪元128（24.87% DM；22.97% DM）（P<0.01）。综上所述，沃锋9全株青贮的RFV最高，且其感官评价、乳酸含量、V-score评分、EE及WSC含量均较高，而氨态氮/总氮（NH3-N/TN）、中性洗涤纤维（NDF）及酸性洗涤纤维（ADF）含量均较低，因此沃锋9为较为优异的粮饲兼用型青贮玉米品种。其次为纪元128，其RFV及48 h NDFD较高，而ADF及木质素（ADL）含量均最低。  相似文献   

13.
In China and throughout the world, evaluation of the nutrient values of forages has always lagged behind research regarding the nutrient requirements of animals. In this study, the effective degradability and availability of forages were evaluated on undegraded and degraded grassland using a technique where nylon bags were incubated in sheep rumen. We found that the concentrations of organic matter (OM), neutral detergent fiber (NDF), non‐fiber carbohydrates (NFC) and carbohydrates (CHO) of forage were higher (P < 0.05) and the crude protein (CP) concentration was lower (P < 0.05) in the degraded grassland than in the undegraded grassland. The effective degradability (ED) of NFC (EDNFC) was higher (P < 0.05) and EDCHO and EDNDF were lower in the undegraded grassland than in the degraded grassland. The EDOM and EDCP were not affected by the type of grassland, which may explain why ED is an inadequate parameter for the evaluation of forage availability. According to an analysis of the ratio of effectively degraded N to OM and NFC, plants of the undegraded grassland can provide more digestive nutrients to meet the requirements of grazing animals compared to plants of the degraded grassland. Results suggested that it is inadequate to consider only chemical composition and effective degradability when evaluating forage availability with grassland degradation. The ratio of effectively degraded N to OM and NFC was an appropriate indicator.  相似文献   

14.
Thirty lactating Beetle‐sannen crossbred goats were randomly divided into 3 groups based on first fortnight milk yield, and then adjusted to body weights to study the effect of fibrolytic enzyme supplementation on digestibility and milk production. Goats in the control group (C) were fed 500 g of concentrate supplement (CS) without exogenous fibrolytic enzymes (control; CG) or CS fortified with cellulase and xylanases @ of 4000 and 12500 (EG1) or 8000 and 18 750 IU/kg (EG2), respectively. CS was offered in two equal proportions just after milking at 7 and 17 h. After feeding CS, goats were fed 1200 g of wheat straw as roughage in two equal parts at 08.00 and 18.00 hours. Milk yield was studied for 3½ months. Milking was done by hand. Changes in body weight were also recorded. After a preliminary period of 60 days of feeding, a 7‐day digestibility trial was conducted in two intervals with 5 animals from each group at a time. The increase in either wheat straw or NDF intake due to the enzyme mixture was only 3%. There was significant improvement (P < 0.05) in the diet digestibility of DM, OM, CP, NDF, ADF and total carbohydrates (TC) between the control and EG1 and EG2 goats. Improvements were greater (P < 0.05) in the latter group. Microbial protein (MBP) was estimated based on purine derivatives and creatinine excreted in spot urine samples. MBP increased about 10 and 15% in EG1 and EG2, but these values were statistically invalid. Phenomenally milk yield, fat and SNF were significantly (P < 0.001) changed with progression of lactation. On a temporal basis, fortnightly changes in FCM yield of this study could be grouped as 1 month each of first and last and 1½ of middle phase of lactation. Repeated measure analysis showed 180 to 260 mL/day of higher FCM and 16 to 20 g/day of higher SNF yield (P < 0.001) in EG2 than control or even EG1 during 5 to 7th fortnight. However, no significant impact (P > 0.05) was noticed on TF. Feed intake, g/kg FCM yield was also 7% less in EG2 than either CG or EG1. The study concluded that apart from increased digestibility, fortification of CS with cellulase and xylanases enzymes improved FCM yield in the last quarter of lactation in goats, and improved body weights by 5% (P > 0.05). While the digestibility of the diet was higher in EG1 and EG2, the impact on FCM and SNF yield was noticed only on EG2. Our study indicated that CS of lactating goats may be fortified with 8000 and 18 500 IU/kg CS in the last quarter of lactation.  相似文献   

15.
One hundred and fifty 7‐day‐old Arbor Acres broilers were randomly assigned into five groups: group 1 served as a control that was fed a basal diet without selenium (Se) supplementation; groups 2, 3 and 4 were fed the basal diet supplemented with 0.15, 0.5 and 1.5 mg Se as Se‐enriched Saccharomyces cerevisiae (SSC) per kg of diet; and group 5 was fed the basal diet supplemented with 0.15 mg per kg of Se as sodium selenite (SS). Growth performance, glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, total antioxidant capacity (T‐AOC), and malondialdehyde (MDA) content in plasma and liver, and cellular glutathione peroxidase (GPX‐1) and phospholipid hydroperoxide glutathione peroxidase (GPX‐4) mRNA levels in liver were determined. Compared with group 1, groups 2–4 exhibited higher body weights (p < 0.05), lower feed/gain ratios, and higher GPX activities in plasma (p < 0.05) and GPX and SOD activities and GPX‐1 and GPX‐4 mRNA levels in liver (p < 0.05). Compared with group 5, group 2 exhibited higher GPX activity in plasma on day 21 (p < 0.05). Compared with group 2 and 5, group 3 exhibited lower MDA content in plasma on day 7 (p < 0.05), higher GPX activity in plasma, SOD activity and GPX‐1 mRNA levels in liver on day 14 and 21 (p < 0.05), and higher GPX‐4 mRNA levels on day 14 (p < 0.05). Compared with group 4, group 3 exhibited lower MDA contents in plasma on day 14 (p < 0.05) and in liver on day 21 (p < 0.05), higher T‐AOC in plasma and higher GPX‐1 mRNA levels on day 14 and 21 (p < 0.05), and higher SOD activity in plasma and higher SOD and GPX activities in liver on day 21 (p < 0.05). Thus, SSC improves growth and antioxidant status of broilers; the short‐term bioavailability of SS was faster than that of SSC, but the long‐term bioavailability of SSC was greater than SS.  相似文献   

16.
The ruminal degradability and intestinal digestibility of dry matter (DM), crude protein (CP) and amino acids (AA) in three total mixed rations with different CP levels were estimated using the modified in vitro three‐step procedure (TSP) and mobile nylon bag (MNB) technique on growing lambs. The ruminal effective degradability of DM and CP did not respond with increasing dietary CP level. However, the intestinal digestibility of DM was significantly increased with increasing dietary CP level estimated by TSP (P < 0.05) or MNB method (P < 0.01). Intestinal digestibility coefficients of CP determined by TSP were lower than those of the MNB method. Histidine was extensively degraded by rumen micro‐organisms, while tyrosine was the most anti‐degradable AA among the samples. The ruminal AA degradability exhibited no significant differences except for threonine, tryptophan, alanine, aspartic acid and proline for the three diets. Similarly, only a few AAs (i.e. histidine, methionine, tryptophan, aspartic acid and cysteine in TSP; histidine, tryptophan, aspartic acid and serine in MNB) had significant differences in their intestinal digestibility; in addition, values of MNB were lower than that of the TSP method, indicating that intestinal digestibility of DM seems to be overestimated in TSP, while that of CP might be overestimated in the MNB method.  相似文献   

17.
This study investigated the effect of cereal grain species (sorghum, wheat, oats and barley), extent of processing (cracked barley, finely ground barley, and wet whole barley) and time of feeding (barley grain mixed with ryegrass hay or fed 2 h before hay was fed), on whole tract dry matter digestibility (WTDMD), and microbial protein synthesis (MPS), as a supplement to ryegrass hay when fed to rumen-cannulated sheep.Expected dry matter digestibility (EDMD) in mixtures of cereal grain and ryegrass hay was calculated by interpolation between in vitro dry matter digestibility (DMD) of each grain and the ryegrass hay. These were compared with measured actual WTDMD to detect positive or negative associative effects. Among grain species, the percentage difference in digestibility between actual WTDMD and EDMD was negative at − 6.6% units for wheat but positive at + 2.3%, + 4.3% and + 5.7% units for sorghum, oats, cracked or finely ground barley, respectively.As expected, the supplementation of sheep fed ryegrass hay with different sources of carbohydrates increased urinary allantoin output (as an indicator of MPS) when compared to sheep fed ryegrass hay alone. The concentration of urinary allantoin was significantly higher in sheep supplemented with sorghum (1916 mg/sheep/day) than wheat, oats or cracked barley ([mean ± S.E.M.] 1451 ± 24 mg/sheep/day) grain. There was a significantly higher urinary allantoin concentration in sheep fed cracked barley compared to finely ground barley or wet whole barley (1479 vs. 1095 vs. 1031 mg/sheep/day, respectively). There was no significant (P > 0.05) difference in urinary allantoin output, expressed as output/kg DM intake, when cracked barley was mixed and fed with the hay or fed 2 h before hay. However, in terms of total output of allantoin this was significantly higher (P < 0.05) (1479 vs. 1209 mg/sheep/day).In sacco degradability characteristics of organic matter and nitrogen for sorghum, oats, wheat, barley at different levels of processing and for ryegrass hay were also measured in the rumen of cannulated sheep.Among grain species, wheat had the highest effective organic matter degradability in the rumen (78.1%) while sorghum had the lowest. The effective degradability of protein of finely ground barley in the rumen was found to be higher than cracked barley or wet whole barley.Wheat grain, being highly degradable in the rumen, had a negative effect on WTDMD. In contrast, sorghum grain, being more slowly degradable in the rumen, would be expected to provide a substantially increased supply of energy to microbes over time in the rumen for MPS.Both cracked barley and finely ground barley also had a positive associative effect on WTDMD when fed with ryegrass increasing it by 5.7% units. The MPS was significantly higher (P < 0.05) in sheep fed cracked barley compared to finely ground barley or wet whole barley. This supports the hypothesis that slowly degrading carbohydrate sources synchronise more closely with available N from degradation of forage in the rumen.  相似文献   

18.
In our previous study, seminal plasma effectively suppressed the induction of sperm to capacitation‐like status and acrosome loss during the thawing process. However, because boar seminal plasma is contaminated with various kinds of bacteria and/or viruses, it is necessary to develop a thawing solution without animal‐derived materials. In this study, we focused on the increase of intracellular Ca2+ ([Ca2+]i) in sperm after thawing and the negative effects of sperm qualities. After thawing, the fluorescent intensity of [Ca2+]i indicator, Fluo‐3/AM, and the level of phosphorylated tyrosine residue of protein were increased in the sperm. Next, we investigated whether the addition of Ca2+ chelators (ethylenediaminetetraacetic acid (EDTA) and ethyleneglycoltetraacetic acid (EGTA)) improved post‐thawed sperm motility. When the frozen–thawed sperm were treated with 6 mmol/L EDTA + 6 mmol/L EGTA, sperm motility was significantly increased as compared with control (6 mmol/L EDTA alone) at all incubation periods (P < 0.05). The combinational treatment significantly suppressed the elevation of [Ca2+]i and the tyrosine phosphorylation, which improved the acrosomal status and fertilizing ability in vitro. Furthermore, when the thawing method was applied for artificial insemination, the fertilization rate was significantly higher than control (P < 0.05, 33% vs. 82%). Thus, we conclude that the addition of EDTA + EGTA to thawing solution is a beneficial tool for artificial insemination using frozen–thawed boar sperm.  相似文献   

19.

Fifteen Bhadawari buffalo heifers of 207?±?9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (N) balance, growth rate, blood metabolites, and puberty. All animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 Mcal/kg) and different protein levels (14.3–22 %). Animals of standard-protein group (SPG) were offered protein and energy as per requirement, while animals of low-protein group (LPG) and high-protein group (HPG) were fed 20 % less and 20 % more protein, respectively, than SPG. Feed dry matter (DM) and metabolizable energy (ME) intake (% body wt. and g/kg w0.75) were similar for all three diets; however, the crude protein (CP) and digestible crude protein (DCP) intake on percent body weight and per kilogram metabolic weight was higher (P?<?0.05) in HPG than in SPG or LPG. Digestibility of CP, cellulose, and hemicellulose was higher (P?<?0.05) in HPG versus LPG. Fecal N excretion was similar, while urinary N excretion was highest (P?<?0.05) in HPG (74.83 g/day) compared with SPG (50.03 g/day) and LPG (47.88 g/day), which resulted in lower N retention in HPG than in the other dietary groups. Level of dietary N had no effect on blood metabolites viz. glucose, urea, and N. Digestible energy (DE) and ME contents of diets were identical, while DCP contents were higher (P?<?0.05) in HPG than in LPG. Feed and nutrient (CP and ME) conversion efficiency to produce a unit kilogram weight gain was identical among the dietary groups. Dietary protein level had no effect on the heifer’s weight and age at puberty. The mean growth rate of heifers at 240 days was higher (P?>?0.05) in SPG (330.8 g/day) than in LPG (296.7 g/day), while the animals gained more weight in January to March months and the lowest weight in May to July months. Protein level had no effect on conception rate of heifers. Results revealed that 20 % higher or less protein than the ICAR requirement had no significant (P?>?0.05) on feed intake, nutrient conversion efficiency for weight gain, heifer growth, and puberty; however, 20 % more protein increased urinary N loss.

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20.
Seventeen crossbred lambs were assigned randomly to low-protein (LP; 8% crude protein [CP]; n = 9) and high-protein (HP; 13% CP; n = 8) diets for 9 weeks. The final body weight, average daily feed intake (ADFI), and average daily gain (ADG) of the HP lambs were significantly higher (P < 0.05) than the LP lambs; however, gain to feed ratio (G:F) for the LP lambs was significantly higher (P < 0.05) than the HP lambs. Hot carcass weight (HCW), adjusted fat thickness, and drip loss of longissimus dorsi (LD) muscle were significantly higher (P < 0.05) for the HP than LP lambs. In contrast, instrumental color values L*, a*, b*, C*, and hue angle (H) of meat from the LP lambs scored significantly higher (P < 0.05) than the HP lambs. The LD muscle from HP lambs had significantly greater CLA of cis-9 trans-11 isomer (P < 0.05) than the LP lambs. The gene expression of metabolism and meat quality-related genes of LP was significantly higher than HP (P < 0.05). These results suggest that a higher dietary CP level promotes growth performance for finishing lambs, whereas lower dietary CP level is beneficial for meat quality, especially when evaluating color characteristics in the final product.  相似文献   

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