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为了考察硫酸黄连素与硫酸粘菌素的联合抑菌作用，对硫酸黄连素和硫酸粘菌素单一和联合应用分别对仔猪大肠杆菌（C83901），猪多杀性巴氏杆菌（C44—1），鸡白痢沙门氏菌（C79—6），禽多杀性巴氏杆菌（C48-1）进行药物敏感性试验；结果：硫酸黄连素对以上4株茵的MIC值分别为：32，128，64，128μ/mL；硫酸粘菌素对以上4株菌的MIC值分别为：64，128，128，256μg／mL；联合应用对上述4株菌的FIC指数分别为：0．375，0．375，0．25，0．5。结果显示上述4株菌对硫酸黄连素与硫酸粘菌素联用的FIC指数均小于0．5，表现为协同作用。二者最佳比例为1：2—1：4，这为复方制剂的开发提供了一定的理论依据。 相似文献
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为研究阿莫西林与硫酸黏菌素对奶牛乳房炎病原菌的体外抗菌活性,本试验选择139株奶牛乳房炎临床分离菌株,采用微量肉汤稀释法,测定了阿莫西林、硫酸黏菌素单用及其联合用药对以上3种主要致病菌的最小抑菌浓度(MIC)。结果:阿莫西林对大肠杆菌、金黄色葡萄球菌和链球菌的MIC范围分别为:2.0~512.0μg/m L、0.125~128.0μg/m L和0.125~1.0μg/m L;硫酸黏菌素对大肠杆菌、金黄色葡萄球菌和链球菌的MIC范围分别为0.5~8.0μg/m L、32.0~128.0μg/m L和64.0~1 024.0μg/m L。二者联用后,对20株奶牛乳房炎大肠杆菌、金黄色葡萄球菌和链球菌FIC平均值分别为0.91、0.85、1.25,对所有菌株的FIC平均值为0.93。结果表明,阿莫西林对链球菌高度敏感;对大肠杆菌及金黄色葡萄球菌适度敏感;硫酸黏菌素对引起奶牛乳房炎的大肠杆菌表现出较高的敏感性,对金黄色葡萄球菌、链球菌的敏感性较低。阿莫西林与硫酸黏菌素联用后,对3种受试菌主要呈现相加作用。 相似文献
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试验旨在研究肠杆菌肽对临床分离大肠杆菌和沙门氏菌的抑菌作用特性。采用药敏纸片法、微量肉汤法测定肠杆菌肽对大肠杆菌、沙门氏菌抑菌圈直径和最小抑菌浓度,通过微量棋盘法测定肠杆菌肽与硫酸粘杆菌素联合抑菌效果,并测定肠杆菌肽对大肠杆菌和沙门氏菌的生长抑制作用。结果显示,肠杆菌肽对鸡源大肠杆菌和沙门氏菌的最小抑菌浓度分别在4～8 mg/L和4～16 mg/L之间;抑菌圈直径在6.0～21.6 mm和6.0～22.5 mm之间。肠杆菌肽与硫酸粘杆菌素的联合抑菌指数（FIC）在1.0～1.5之间。研究表明,肠杆菌肽对大肠杆菌和沙门氏菌具有良好的抑菌作用,与硫酸粘杆菌素无联合抑菌效应。 相似文献
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采用磺胺间甲氧嘧啶（SMM）与恩诺沙星（EFs）联合用药对黄羽肉鸡常见病原菌大肠杆菌、沙门氏菌和禽多杀性巴氏杆菌进行了体外药物敏感性试验。结果表明,SMM对3种病原菌的MIC值分别为256、1 024、256μg/m L;EFs对3种病原菌的MIC值分别为32、16、2μg/m L。SMM与EFs联合药敏试验结果表明对大肠杆菌和禽多杀性巴氏杆菌呈现协同作用,对沙门氏菌呈现相加作用。 相似文献
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应用试管二倍稀释法测定了氟砜霉素、氯霉素、TMP和OMP对15株畜禽常见病原菌的最小柳菌浓度（MIC）。结果表明，氟砜霉素的体外抗菌活性强，优于或等于氯霉素；TMP和OMP的体外抗菌活性偏低，但OMP优于TMP。氟砜霉素和氯霉素与TMP、OMP对14株细菌的联合药敏试验经肉汤稀释棋盘法测定，结果表明，氟砜霉素和氯霉素分别与TMP、OMP体外联合，抑菌效果相同，呈协同作用的均占50%与28.6%，即TMP或OMP对氟砜霉素和氯霉素的增效作用相等，但TMP优于OMP。在14株细菌对氟砜霉素和氯霉素分别与TMP、OMP的总共56组体外联合试验中，呈协同作用的占39.3%，主要为大肠杆菌、多杀性巴氏杆菌、沙门氏菌及金黄色葡萄球菌；无关作用的点60.7%；无拮抗现象。故临床联合用药前，仍需进行联合药敏试验，呈协同作用者才可联用。 相似文献
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《中国兽医学报》2014,(12):1967-1970
本试验初步探讨秦皮水提取物与抗菌药联合诱导含fosA3型耐药基因大肠杆菌传代的体外抑菌活性,为耐药大肠杆菌感染疾病提供治疗参考方案。采用二倍微量稀释法体外分别检测秦皮水提取物与抗菌药物的最小抑菌浓度(MIC),并以1/2 MIC的秦皮水提取物与抗菌药联合诱导含(fosfomycin resistance gene,FosA3)型大肠杆菌的传代试验。秦皮水提取物的MIC为1.0g/mL,以0.5g/mL的秦皮水提取物与头孢曲松钠、阿莫西林、诺氟沙星、粘杆菌素、痢菌净、磺胺间甲氧嘧啶、阿米卡星、头孢他啶、头孢噻呋钠、氟苯尼考和磷霉素11种抗菌药联合诱导传代后MIC显著降低。结果显示:秦皮水提取物联合抗菌药诱导耐药细菌体外抗菌活性明显增强,对含fosA3型大肠杆菌具有耐药逆转作用。 相似文献
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氟苯尼考与多种抗菌药物联合应用对大肠杆菌的体外抑菌试验 总被引：4，自引：1，他引：3
为了解氟苯尼考分别与恩诺沙星、TMP和多西环素联合用药的体外抑菌效果,本试验采用倍比稀释法,测定了氟苯尼考、恩诺沙星、TMP和多西环素对大肠杆菌O78的最小抑菌浓度(MIC);采用棋盘法进行恩诺沙星、TMP、多西环素分别与氟苯尼考联合应用对大肠杆菌的药敏试验。结果表明:四种抗菌药物都有较强的抑菌能力,氟苯尼考MIC为4μg/mL,恩诺沙星MIC为0.1μg/mL,TMP的MIC为16μg/mL,多西环素的MIC为4μg/mL。氟苯尼考与恩诺沙星联合表现为无关作用,与TMP联合表现为协同作用,与多西环素联合表现为累加作用。 相似文献
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安普霉素及其联合用药对金黄色葡萄球菌体外PAE的研究 总被引：1，自引：0，他引：1
本试验采用稀释法去除抗生素。用菌落计数测定细菌生长曲线的方法,分别测定了安普霉素及其与阿莫西林或氨苄西林联用对金黄色葡萄球菌的体外抗生素后效应（PAE）.当药物以2×MIC、4×MIC和8xMIC浓度作用于金黄色葡萄球菌时,安普霉素的体外PAE分别为1．78h、2．49h、3．63h;安普霉素与阿莫西林联用的体外PAE分别为4．13h、6．91h、9．82h;安普霉素与氨苄西林联用的体外PAE分别为3．81h、5．67h、8．45h。结果表明。安普霉素在体外对金黄色葡萄球菌有较长的PAE。且随药物浓度的升高其PAE也相应地延长．呈明显的剂量依赖性;安普霉素与阿莫西林、氨苄西林联用对金黄色葡萄球菌的体外PAE呈现相加或协同作用。 相似文献
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抗生素与中草药联用对猪链球菌体外抑菌活性分析 总被引：1，自引：1，他引：0
为观察中草药与抗菌药物联用对猪链球菌的体外抑菌效果,用试管二倍稀释法,分别测定4种中草药水提取物、4种抗菌药物及中草药和抗菌药物的多个配伍对猪链球菌的最小抑菌浓度（MIC）。结果显示,所选中草药抗菌药物及其联合用药对猪链球菌均有较好的抑制作用,且联合用药的抗菌作用表现为部分增强,其中对猪链球菌作用最明显的配伍是黄柏＋氨苄西林、石榴皮＋氨苄西林,野菊花＋氨苄西林、中药复方＋氨苄西林、石榴皮＋青霉素G和黄柏＋壮观霉素,其最小抑菌浓度为0.977 mg/mL。 相似文献
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Tanigawa M Sawada T 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(9):436-442
The pharmacodynamic effects of amoxicillin against Actinobacillus pleuropneumoniae at exposure concentration above and below minimum inhibitory concentration (MIC) were evaluated in both in vitro and in vivo. In vitro, the growth and morphological change of A. pleuropneumoniae in culture medium was observed. In vivo, the efficacy of amoxicillin on experimentally induced A. pleuropneumoniae infection in disease-free pigs was evaluated. Fifteen pigs were divided into three groups (n = 5 per group). After the onset of clinical respiratory disease symptoms, 6 h post-infection, amoxicillin sustained-release injectable formulation was injected intramuscularly at 7.5 mg/kg/day (group I) and 15 mg/kg/day (group II). Then the serum concentration of amoxicillin was measured. An untreated infected group served as controls. In each amoxicillin administration group, if symptoms were not absent after 48 h, the pig was injected with the amoxicillin sustained-release injectable formulation again using the same dosage. In vitro, the growth of A. pleuropneumoniae inhibited by amoxicillin exposure at the concentration above the MIC (1.28 x MIC), and the inhibition time was in directly proportion to the time of amoxicillin exposure. Moreover, all the cells were lysed. Whereas the bacterial growth inhibition at the amoxicillin exposure concentration below the MIC (0.25 x MIC) was not done, and the shape of cells were normal or long filamentous. In vivo, the group I clinical and pathological score was higher than the group II, and the group I weight gain was significantly less than the group II. Performance with respect to weight gain corresponded with clinical signs. The infected control group was severely affected with an 80% (4/5) mortality rate 24-96 h post-challenge. The duration of time above MIC (T > MIC) of serum amoxicillin concentration in the group I was less than group II. The present studies suggest that amoxicillin has exposure time-dependent bactericidal activity against A. pleuropneumoniae. 相似文献
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为了评价中药及其复方对大肠杆菌的体外抑菌效果,取乌梅、五倍子、山茱萸、黄芩等30种中药,通过试管二倍稀释法测定中药水提物对大肠杆菌的最小抑菌浓度（MIC）与最小杀菌浓度（MBC）,并筛选出抗菌作用较强的中药水提物与其他中药水提物组成复方,测定中药复方对大肠杆菌的体外抑菌效果。结果表明,五倍子、乌梅、金银花、大青叶、黄芩、薄荷、五味子、青蒿、秦皮对大肠杆菌有明显的抑制作用,其中五倍子对大肠杆菌的抑菌活性最强（MIC为6.25 mg/mL）,而五味子和乌梅对大肠杆菌杀菌活性最强（MBC为50 mg/mL）。选取9种中药水提物作为主药与其他中药水提物分别组成复方后,大部分复方对大肠杆菌的抑制作用明显增强。 相似文献
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百草肠毒清由白头翁、穿心莲、石榴皮等9味中药按一定比例配制而成。对架子猪、羔羊腹泻的防治试验结果表明,百草肠毒清、硫酸新霉素、硫酸粘菌素与空白对照组相比,治愈率均差异极显著（P〈0.01）;百草肠毒清组与硫酸新霉素组比较,治愈率与有效率均差异极显著（P〈0.01）;百草肠毒清组与硫酸粘菌素组比较,治愈率差异显著（P〈0.05）,有效率差异极显著（P〈0.01）;百草肠毒清组与肠毒清＋新霉素组和肠毒清＋粘菌素组相比较,治愈率及有效率均无显著性差异。临床试验证明,单独使用百草肠毒清对家畜腹泻疗效确切。 相似文献
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硫酸粘杆菌素(Colistin sulfate)对大肠杆菌的抗生素后效应 总被引：7，自引：0，他引：7
用微量稀释法潮定的硫酸粘杆菌素(colistinsulfate)对4株大肠杆菌的最小抑茵浓度(MIC)和最低杀菌浓度(MBC)。分剐为0．2～O．8mg／k和0．8～1．6mg／k。2MIC硫酸粘杆菌素对4株大肠杆菌作用2h后。l000倍稀释去除药物。硫酸粘杆菌素对4株大肠杆菌的抗生素后效应(PAE)在1．062-2．017h之间。64、32、16、8、4、2MIC硫酸粘杆菌素对大肠杆菌CMCC44103作用2h后。l000倍稀释去除药物。硫酸粘杆菌素PAE的平均值分别为0．517、2．487、2．037、1．727、1．467、1．057h。PAE与药物浓度呈正相关。 相似文献
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Tanigawa M Sawada T 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(10):513-518
The bactericidal effects of amoxicillin at below minimum inhibitory concentration (MIC) against Actinobacillus pleuropneumoniae NB001 were studied in vitro and in vivo. In vivo, the efficacy of amoxicillin on experimentally induced A. pleuropneumoniae infection in disease-free pigs was evaluated. Nine pigs were divided into three groups and all three groups were housed in the same room. Group I pigs were given long-acting amoxicillin injection 22 h prior to A. pleuropneumoniae challenge. Group II pigs were also A. pleuropneumoniae challenged but not given long-acting amoxicillin. Group III pigs were not treated. In vitro, A. pleuropneumoniae growth was suppressed in porcine blood with amoxicillin at below MIC. In vivo, clinical signs of disease were absent or mild in group I during 50 h post-challenge, and serum amoxicillin concentration was already less than MIC from 15 h post-challenge. Infected group II controls were severely affected by the infection, and mortality reached 100% within 50 h post-challenge. All non-treated pigs in group III became infected with NB001 from infected control pigs, and they displayed severe clinical signs of disease within 24 h post-challenge of groups I and II, and died within 50 h post-challenge of groups I and II. 相似文献
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Tatsuya Nakayama Michio Jinnai Ryuji Kawahara Khong Thi Diep Nguyen Nam Thang Tran Thi Hoa Le Kieu Hanh Pham Ngoc Khai Yoshinori Sumimura Yoshimasa Yamamoto 《Tropical animal health and production》2017,49(1):31-37
Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use selects for strains of colistin-resistant ESBL-E. With the cooperation of seven local households, we detected ESBL-E in chickens’ feces after treating chickens with CBD. Phylogenetic groupings and the presence of CTX-M/AmpC genes were determined, and the multi-antibiotic susceptibility of isolates was analyzed. Our results showed that ESBL-E presented in seven chickens’ feces from two households. Seventy-two percent of ESBL-E isolates harbored CTX-M9 and the phylogenetic group A; the colistin minimum inhibitory concentration (MIC) of all isolated ESBL-E ranged from 0.064 to 1 μg mL?1. Moreover, ESBL-E isolates were used to experimentally select for colistin resistance, and the effect of commercial CBD on ESBL-E was investigated. The results showed that an ESBL-E strain with a colistin MIC of 4 μg mL?1 was able to grow in media with CBD. Although CBD treatment was effective, in vitro experiments demonstrated that ESBL-E can easily acquire colistin resistance. Therefore, restrictions on colistin use are necessary to prevent the emergence of colistin-resistant bacteria. 相似文献
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味蒙药对种仇清型牛源致病性大肠杆菌体外抑菌及联合抑菌效果的研究 《畜牧与饲料科学》2018,39(10):102-107
为研究蒙药对3种血清型牛源致病性大肠杆菌（Escherichia coli，E. coli）的体外抑菌效果，采用牛津杯法测定16味蒙药对受试菌株的抑菌圈直径，利用微量稀释法结合平板法测定最小抑菌浓度（MIC）和最低杀菌浓度（MBC），采用棋盘法测定敏感蒙药的协同指数（FICI）。结果表明，乌里史古—达兰—哈力苏、协日—洪连、乌拉勒吉嘎纳对3种血清型大肠杆菌的抑菌圈直径为16.10-29.64 mm，MIC为7.81-31.25 mg/mL，MBC为15.63-125 mg/mL。联合抑菌试验结果表明，乌拉勒吉嘎纳与协日—洪连，舒布格日—希木勒德格与呼仍—图如对E. coli O26的FICI为0.50，乌拉勒吉嘎纳与乌里史古—达兰—哈力苏对E. coli O83的FICI为0.50。结果提示，乌里史古—达兰—哈力苏、协日—洪连、呼仍—图如、乌拉勒吉嘎纳、阿纳尔对3种血清型大肠杆菌的综合抑菌效果较好；舒布格日—希木勒德格与呼仍—图如在抑制E. coli O26时表现为协同作用，组方时可优先考虑；乌拉勒吉嘎纳与乌里史古—达兰—哈力苏在抑制E. coli O83时表现为协同作用，组方中可优先考虑。 相似文献