共查询到19条相似文献，搜索用时 703 毫秒
1.
《中国兽医学报》2015,(9):1456-1462
为确定广东省增城某猪场产房仔猪暴发渗出性皮炎的致病菌,从病猪心脏、肺脏、病变皮肤分离并纯化细菌,经形态学观察,镜检,生化特性及gap基因的序列分析,确定为猪葡萄球菌,命名为ZC-4株。药敏试验结果显示该菌株对头孢拉定、头孢唑啉、新生霉素高度敏感,对头孢噻肟、万古霉素、庆大霉素中度敏感,对青霉素、阿莫西林、磺胺类药物、链霉素、恩诺沙星等均耐药。对分离株毒素基因进行检测,结果显示,分离株携带ExhA毒素基因,测序结果表明,其编码区全长为822bp,编码274个氨基酸;氨基酸序列与丹麦分离株ExhA基因(AF515453)的同源性最高,为99.6%;遗传进化分析显示,分离株所携带的毒素基因与参考菌株猪葡萄球菌ExhA毒素基因聚类紧密,位于一个小的分支内。分离株致病性试验结果显示,分离株接种25日龄健康断奶仔猪后24h,病猪耳根、四肢内侧、背部、腹部、臀部、尾根等部位出现蜕皮并露出有液体渗出的鲜红创面,继而皮肤形成一层厚厚的结痂,说明该菌株具有较强的致病性。本试验为猪葡萄球菌疫苗的研制及致病机制研究提供了理论依据。 相似文献
2.
为建立研究仔猪渗出性皮炎发病机制及治疗制剂的动物模型,本研究采用PCR及BLAST序列比对方法对猪葡萄球菌437-2株毒素基因型进行鉴定,采用纸片扩散法检测其耐药性,通过不同浓度菌液人工感染仔猪试验分析该菌株的临床致病力、最小致病浓度、发病周期及组织病理学变化。结果显示,该分离菌株为ExhD毒素基因型,其毒素基因与德国分离株(GenBank登录号:AM94662.1)和俄罗斯分离株(GenBank登录号:AM950188.1)的ExhD基因同源性达99%;药敏试验结果显示,该菌株为多重耐药菌株,对青霉素、磺胺异噁唑、链霉素、阿奇霉素、红霉素、四环素、杆菌肽及诺氟沙星耐药;仔猪致病性试验结果显示,低剂量组在整个试验周期内仅注射部位表现出皮炎症状,而中、高剂量组在耳后皮下注射2 d后均出现显著的渗出性皮炎症状,病猪表现为耳后出现油皮,全身被毛粗糙,皮肤呈深褐色厚皮痂,有大片蜕皮和黄色液体渗出;感染仔猪的脏器和皮肤病理切片结果显示,低剂量组仔猪脏器无异常,耳部皮肤角质层角化过度,炎性细胞浸润,无棘皮层细胞分离,而中、高剂量组感染仔猪脏器具有一定程度的损伤,皮肤表皮棘细胞层出现明显的细胞分离。上述试验结果说明,利用猪葡萄球菌437-2株可成功建立仔猪渗出性皮炎模型,所需的最小攻菌浓度为1×10~9 CFU/mL。 相似文献
3.
4.
为建立研究仔猪渗出性皮炎发病机制及治疗制剂的动物模型,本研究采用PCR及BLAST序列比对方法对猪葡萄球菌437-2株毒素基因型进行鉴定,采用纸片扩散法检测其耐药性,通过不同浓度菌液人工感染仔猪试验分析该菌株的临床致病力、最小致病浓度、发病周期及组织病理学变化。结果显示,该分离菌株为ExhD毒素基因型,其毒素基因与德国分离株（GenBank登录号：AM94662.1）和俄罗斯分离株（GenBank登录号：AM950188.1）的ExhD基因同源性达99%;药敏试验结果显示,该菌株为多重耐药菌株,对青霉素、磺胺异噁唑、链霉素、阿奇霉素、红霉素、四环素、杆菌肽及诺氟沙星耐药;仔猪致病性试验结果显示,低剂量组在整个试验周期内仅注射部位表现出皮炎症状,而中、高剂量组在耳后皮下注射2 d后均出现显著的渗出性皮炎症状,病猪表现为耳后出现油皮,全身被毛粗糙,皮肤呈深褐色厚皮痂,有大片蜕皮和黄色液体渗出;感染仔猪的脏器和皮肤病理切片结果显示,低剂量组仔猪脏器无异常,耳部皮肤角质层角化过度,炎性细胞浸润,无棘皮层细胞分离,而中、高剂量组感染仔猪脏器具有一定程度的损伤,皮肤表皮棘细胞层出现明显的细胞分离。上述试验结果说明,利用猪葡萄球菌437-2株可成功建立仔猪渗出性皮炎模型,所需的最小攻菌浓度为1×109 CFU/mL。 相似文献
5.
仔猪渗出性皮炎(Exudative Epidermitis,EE),也叫猪油皮病,是一种以表皮脱落、皮肤油脂样渗出以及体表结痂为特征的体表接触性传染病.该病多发于寒冷潮湿的冬春季节,哺乳仔猪及刚断奶保育猪多发,通常散发或小范围群发于猪群中,虽然发病率不高,但治愈率低,耐过猪生长速度受到严重影响.仔猪渗出性皮炎的病原主要为葡萄球菌,包括猪葡萄球菌、松鼠葡萄球菌[1].主要的致病因素是病原菌侵入血液循环造成的败血症及细菌繁殖产生的表皮脱落毒素.本文主要报道在临床见到1例仔猪渗出性皮炎,通过临床症状观察及流行病学调查初步判断为仔猪渗出性皮炎,实验室检验分离到1株细菌,经生化试验,PCR分子鉴定,基因序列分析,确定为猪葡萄球菌.我们通过药敏试验筛选敏感药物,清洁环境,结合其他有效的综合防控措施,控制了该病的蔓延. 相似文献
6.
猪渗出性皮炎又称葡萄球菌性皮炎、皮脂溢性皮炎,俗称＂猪油皮病＂,是由能产生表皮脱落毒素的猪葡萄球菌引起的哺乳仔猪和刚断奶仔猪的一种急性和超急性接触传染性皮肤病,一般呈散发性,对个别猪群可造成很大损失。 相似文献
7.
8.
9.
王俊如 《畜牧兽医科技信息》2021,(1)
仔猪渗出性皮炎(EE)又称猪油皮病,主要是由猪葡萄球菌感染引起的一种以仔猪全身油质样渗出为发病特征的高度接触性传染病,严重影响仔猪的生长发育并导致死淘率增加。在阐述仔猪渗出性皮炎流行特点、临床症状和剖检病变的基础上,提出了诊治方法和预防措施,以期为养猪场户提供有益参考。1流行特点1.1猪葡萄球菌通常寄生在仔猪皮肤和黏膜表面,该菌属分泌的表皮脱落毒素是引起本病的主要致病因子。本病的发病率为10%~30%,死亡率为50%~80%,仔猪日龄越小,病死率越高。 相似文献
10.
11.
The prevention of exudative epidermitis could be confirmed in experimental investigations with gnotobiotic piglets when the skin first was colonized with avirulent strains of Staphylococcus (Staph.) hyicus and subsequently exposed to virulent strains of Staph. hyicus. However, locally restricted cutaneous lesions in the area of application corresponding to exudative epidermitis were seen in five of nine piglets. Using the strain Staph. sciuri the spread of virulent Staph. hyicus could not be suppressed. Such infected two piglets developed generalized exudative epidermitis. In another experiment with four piglets it could be shown, that the relative protective mechanism correlating to bacterial interference on the one hand can be influenced by the virulence of causative organisms. On the other hand it even can be abolished when skin lesions are involved. For that reason probably the utilization of bacterial interference in prevention of exudative epidermitis under field conditions is considerably limited. 相似文献
12.
Staphylococcus hyicus virulence in relation to exudative epidermitis in pigs. 总被引：3，自引：0，他引：3 
下载免费PDF全文
下载免费PDF全文 Staphylococcus hyicus strains with different phage types, plasmid profiles, and antibiotic resistance patterns were isolated from piglets with exudative epidermitis. The strains could be divided into virulent strains, producing exudative epidermitis, and avirulent strains, producing no dermal changes when injected in experimental piglets. The results showed that both virulent and avirulent strains were present simultaneously on diseased piglets. This constitutes a diagnostic problem. Concentrated culture supernatants from nine virulent strains injected in the skin of healthy piglets produced a crusting reaction in all piglets. Acanthosis was observed in the histopathological examination of the crustaceous skin. Concentrated culture supernatants from nine avirulent strains produced no macroscopic or microscopic skin changes. Protein profiles from all virulent strains and seven out of nine avirulent strains showed a high degree of protein band homology. An approximately 30 kDa protein present in all concentrated culture supernatants capable of producing skin changes, could not be detected in samples that did not produce skin changes. No other protein showed a similar association. It is concluded that crusting reaction of piglet skin is a suitable indicator of virulence in S. hyicus in relation to exudative epidermitis, and that virulent strains produce a 30 kDa protein, absent in concentrated culture supernatants from avirulent strains. This 30 kDa protein might be an exfoliative toxin. 相似文献
13.
Staphylococcus chromogenes is closely related to Staphylococcus hyicus, which is recognised as the causative agent of exudative epidermitis (EE) in pigs. S. chromogenes is part of the normal skin flora of pigs, cattle and poultry and has so far been considered non-pathogenic to pigs. A strain of S. chromogenes producing exfoliative toxin type B, ExhB, was identified by the use of a multiplex PCR specific for the exfoliative toxins from S. hyicus. The exfoliative toxin from S. chromogenes reacted in immunoblot analysis with polyclonal and monoclonal antibodies specific to ExhB from S. hyicus and had an apparent molecular weight of 30 kDa. Sequencing the gene encoding the exfoliative toxin from S. chromogenes revealed that the molecular weight of the toxin with the signal peptide and the mature toxin was 30,553 and 26,694 Da, respectively. Comparison of the exhB genes from S. chromogenes strain VA654 and S. hyicus strain 1289D-88 showed differences in seven base pairs of the DNA sequences and in two amino acid residues in the deduced amino acid sequences. Pigs were experimentally inoculated with S. chromogenes strain VA654. By clinical observations and histopathological evaluation of the skin alterations, all pigs revealed development of generalized exudative epidermitis. No toxin producing S. hyicus was isolated from the pigs and all ExhB-positive bacterial isolates were identified as S. chromogenes. This confirmed that the disease-causing agent was the inoculated S. chromogenes strain VA654. The results of this study show that S. chromogenes may cause exudative epidermitis in pigs. 相似文献
14.
Kanbar T Voytenko AV Alber J Lämmler C Weiss R Zschöck M Shilov IA Dmitrenko OA Gintsburg AL 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(9):429-433
In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n=23) and Germany (n=17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia. 相似文献
15.
Isolation of exfoliative toxin from Staphylococcus hyicus subsp. hyicus and its exfoliative activity in the piglet 总被引：7，自引：0，他引：7
H Sato T Tanabe M Kuramoto K Tanaka T Hashimoto H Saito 《Veterinary microbiology》1991,27(3-4):263-275
Exfoliative toxin was isolated from the sterile cell-free filtrate of 24 h culture of Staphylococcus hyicus subsp. hyicus strain P-1. The partial purification of exfoliative toxin produced by S. hyicus (shET) was performed by precipitation with 50-80% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column and column chromatography on DEAE-cellulose. Partially purified shET (pp-shET) caused exfoliation in piglets at 8 to 12 h after intradermal or subcutaneous injection. However, heat-treated pp-shET did not cause exfoliation in piglets for up to 24 h after injection. On histopathological examination of the skin at 12 h after injection of pp-shET, an intraepidermal cleavage plane was shown between the stratum corneum and stratum granulosum and at the stratum granulosum. 相似文献
16.
为分析猪葡萄球菌脱落毒素EXHC和SHETA基因结构并预测其所编码蛋白的结构和功能,本试验根据GenBank已报道的猪葡萄球菌脱落毒素EXHC和SHETA基因序列分别设计了1对特异性引物,从猪葡萄球菌GDZC株中扩增获得EXHC和SHETA基因片段,大小分别为1007和971 bp。序列分析结果表明,EXHC基因与猪葡萄球菌丹麦分离株(GenBank登录号:AF515455)及猪源松鼠葡萄球菌河北分离株(GenBank登录号:JF755400)的核苷酸序列、氨基酸序列同源性均为100.0%,而与其他葡萄球菌脱落毒素基因的核苷酸、氨基酸序列同源性分别为3.3%~53.9%和7.9%~44.2%。SHETA基因与日本分离株(GenBank登录号:AB036768)的核苷酸序列同源性为96.2%,氨基酸序列同源性为98.4%,在保守区共有31个碱基发生突变。利用DNAStar软件对EXHC和SHETA蛋白结构进行分析,结果显示EXHC基因编码的蛋白为亲水性蛋白,SHETA基因编码蛋白为疏水性蛋白,抗原性较差。本研究从猪葡萄球菌GDZC株中成功扩增获得EXHC和SHETA基因片段并对其编码的蛋白结构进行了预测,证实了中国分离的猪葡萄球菌同时携带有EXHC和SHETA 2种毒素基因,为进一步研究猪葡萄球菌的致病机理及毒素之间的相互作用提供了理论依据。 相似文献
17.
根据GenBank已发表的猪葡萄球菌的6种脱落毒素基因序列,设计合成了6对相应的特异性引物,通过特异性、敏感性和重复性试验建立了可行的多重PCR检测方法。用该方法对临床分离到的9株猪葡萄球菌进行检测,均扩增出了与预期大小相符的23SrDNA（662bp）条带;同时,其中6株分别扩增出了EXHA（316bp,2株）、EXHC（525bp,2株）和Shet-A（814bp,2株）基因特异性条带;另外3株均未扩增出任何毒素基因特异性条带,鉴定为无毒力菌株,以上结果与生化鉴定及单一PCR检测测序结果一致。结果表明,本试验所建立的多重PCR方法不仅操作快速方便、节约试验成本,而且具有高度特异性、敏感性和良好的重复性,可用于仔猪渗出性皮炎的诊断和猪葡萄球菌的快速鉴定。 相似文献
18.
Isolation and restriction endonuclease analysis of a tetracycline resistance plasmid from Staphylococcus hyicus 总被引：3，自引：0，他引：3
A plasmid of 4.550 kb, conferring resistance to tetracycline, was demonstrated in Staphylococcus hyicus cultures from piglets with exudative epidermidis. The plasmid-encoded properties were determined both by curing and interspecific protoplast transformation experiments. The tetracycline resistance (TET) plasmid, designated pST1, was characterized by restriction endonuclease analysis and a preliminary restriction map was constructed. The pST1 plasmid was demonstrated in 19 (57.6%) of 33 S. hyicus cultures by Southern blot hybridization. It was also detectable by electron microscopy. 相似文献
19.
The objective of the study was to analyze changes in peripheral blood leukocyte subsets in cases of naturally occurring exudative epidermitis (EE) in pigs. Five of ten piglets developed the chronic clinical form of EE 2-5 days after weaning (PW). Blood samples were obtained at 7, 14 and 21 days from both normal and clinically affected piglets for routine haematology and for the determination of CD45, CD21, CD4, CD8 and gammadeltaTCR cell markers by flow cytometry. When compared with clinically normal piglets EE affected pigs showed significantly decreased values of monocytes at 14 and 21 days PW, and increased numbers of neutrophils and leukocytes at 21 days PW. The EE affected pigs also had an early significant CD4(+) and CD8(high+) T lymphocyte proliferative response at 7 days PW. However affected pigs had a significantly reduced number of B (CD21(+)) and gammadeltaTCR(+) T lymphocytes in blood at 21 days PW. Although all values remained within the normal range, the significant differences in some peripheral blood leukocyte subsets between the two groups of piglets suggest that the generalised cutaneous infection with Staphylococcus hyicus is severe enough to induce a systemic inflammatory and immune responses. 相似文献