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1.
Two hundred bovine mastitis streptococcal strains belonging to groups B, C, E, G and L were tested comparatively by means of the co-agglutination and precipitation technique. Identical results were obtained with the two tests in 191, or 95.5 %, of the strains. Six strains, which could not be grouped by co-agglutination, proved to belong to group B when grouped by precipitation. On the other hand, one strain which proved to belong to group G and two strains to group L when grouped by co-agglutination, could not be grouped by precipitation. Some cross-reactivity was observed between groups A and C, B and G, B and L. Only a few L strains showed marked cross-reactivity which was not easily distinguished from specific reactions. However, the cross-reactivity ought to be eliminated by dilution or adsorption. Using the precipitation test as a supplementary method, the easy and rapid co-agglutination test was found to be a suitable procedure for routine grouping of mastitis streptococci.  相似文献   

2.
建立了8株抗猪囊虫循环抗原(CA)的杂交病细胞系。其中6F12和2E7为抗囊虫特异McAb;McAb 1C6、1C7、1B5、4D9、2B9和8D8与囊虫、(?)球蚴、细颈囊尾蚴抗原均可发生反应。这些McAb的腹水ELISA效价为105～107,细胞培养上清液效价为102,并均可与病猪血清中的囊虫CA反应形成沉淀线。用1B5、6F12和8D8分别致敏血球,以反向间接血凝试验检测98份囊虫病猪血清,检出率分别为70.41%(69/98)、6.12%(6/98)和7.14%(7/98)。本研究制备的McAb可用于猪囊虫循环抗原检测。  相似文献   

3.
乌骨鸡蛋形指数对孵化率的影响   总被引：8，自引：0，他引：8  
将乌骨鸡种蛋按蛋形指数分为A(≤ 72 0 % )、B(72 1 %～ 74 0 % )、C(74 1 %～76 0 % )、D(76 1 %～ 78 0 % )、E(≥ 78 1 % ) 5组进行孵化 ,结果表明 :5组乌骨鸡的受精蛋孵化率分别为 82 6%、86 1 %、90 2 %、87 2 %、82 5% ,C组显著高于A、B、D、E各组 (P <0 0 5) ,并极显著地高于A组和E组 (P <0 0 1 ) ,蛋形指数为 74 1 %～ 76 0 %的乌骨鸡种蛋孵化效果最佳  相似文献   

4.
After intramuscular (IM) administration (dose 20 mg/kg) of three 20% (Terramycin/LA (product A), Alamycin LA (product B) and Terralon 20% LA (product C) and two 10% oxytetracycline (OTC) formulations (Engemycin 10% (product D) and Oxyject 10% (product E)), to calves, pigs and sheep, the OTC residue concentrations were determined in organs, muscle, fat, plasma, urine and at the injection sites at 10 days post injection (p.i.). At that time the irritation at the injection site was studied, too. The three 20%-formulations (products A, B, C) and one 10%-formulation (product E) induced considerable local irritation in and between the muscles. This was most pronounced in calves and pigs; in sheep the extent of irritation was limited. Ten days after administration of formulations A, B, C and E, OTC residues were found in organs and the OTC recovery at the injection sites varied widely among the three species. Following IM injection of product D minimal tissue irritation and no OTC residues could be detected at the injection site at 10 days p.i. The differences in local tissue irritation and the residue state of the carcass (including injection site) are related to the various solvent systems used in the formulations.  相似文献   

5.
Enzyme immunoassays (EIAs) for the determination of autoanti-bodies (AA) to thyroid antigens in canine serum were developed. Streptavidin (SA) was immobilized as capture molecule on microtitreplates (MTP). Thyroglobulin (Tg) purified from canine thyroids and the thyroid hormones thyroxine and triiodothyronine (T3 and T4) were conjugated to biotin labelling reagents and attached to the MTP over the SA-biotin bridge. Bound AA were detected with anti-dog-immunoglobulin G (IgG) labelled with horseradish peroxidase. Serum samples from dogs which were allotted to four groups were analysed: A (n = 31), biochemical evidence of hypothyroidism; B (n = 76), clinical signs of hypothyroidism; C (n = 47), euthyroid with non-thyroidal disease; D (n = 186), clinically healthy. The validity of the assays was tested with two different methods. After thiophilic absorption chromatography of positive sera, a positive reaction in the EIA was only detected in those fractions which coeluted with the canine IgG standard. Furthermore, the positive reaction was blocked by the addition of the corresponding antigen. In 55% of the hypothyroid dogs AA to Tg and/or T3 and T4, respectively, were found (up to a titre of 1 : 1600). In group B 34% of the dogs were diagnosed positive, but the titre was lower (up to 1 : 400). In the groups C and D the number of dogs with AA and their titre was significantly lower. Two different methods for distinguishing positive and negative test results were compared in order to increase the specificity of the tests without decreasing the sensitivity. The EIAs are precise and based on high agreement with previous reported assays able to discriminate dogs with thyroiditis from healthy ones. These assays represent a good alternative to the isotope assays generally used for the analysis of AA to T4 and T3.  相似文献   

6.
本试验以发酵菜籽粕等氮替代不同比例豆粕,研究其对肉鸡生长性能、营养物质消化吸收及肉品质的影响。选取200只7日龄健康爱拔益加(AA)肉仔鸡,随机分为4组,每组设置5个重复,每个重复10只鸡(公母各占1/2)。A组为对照组,饲喂基础饲粮;B、C、D组为试验组,分别用发酵菜籽粕等氮替代基础饲粮中15%、35%和50%的豆粕进行饲喂。试验从7日龄开始,至42日龄结束。结果显示:1)7~42日龄阶段,C、D组平均日增重均极显著低于A组(P0.01),料重比均极显著高于A组(P0.01),同时C组的平均日采食量极显著高于A组(P0.01);B组的平均日增重、平均日采食量和料重比与A组差异不显著(P0.05)。2)42日龄时,B、C、D组肠道食糜pH较A组分别下降1.57%(P0.01)、1.41%(P0.01)、0.16%(P0.05);B、C、D组肠道食糜黏度较A组分别下降4.17%(P0.05)、4.17%(P0.05)、1.67%(P0.05)。42日龄时,与A组相比,B组蛋白质表观消化率、能量表观消化率、脂肪表观消化率显著或极显著上升(P0.05或P0.01),而C、D组蛋白质表观消化率、脂肪表观消化率则显著或极显著下降(P0.05或P0.01)。3)与A组比,各试验组胸肌的pH_(45 min)、pH_(24 h)出现不同程度的下降,但B组pH_(24 h)与A组差异不显著(P0.05);与A组相比,B组胸肌的亮度(L~*)值极显著提高(P0.01),红度(a~*)、黄度(b~*)值则极显著降低(P0.01);各试验组胸肌的滴水损失极显著低于A组(P0.01);B、C组胸肌的剪切力极显著低于A组(P0.01);B、C、D组胸肌的肌苷酸含量较A组分别提高2.55%(P0.01)、0.66%(P0.05)、0.21%(P0.05)。综上,发酵菜籽粕等氮替代肉鸡饲粮中15%的豆粕对生长性能无负面影响,同时还可促进肉鸡对营养物质的消化吸收并改善肉品质。  相似文献   

7.
To determine the effects of burdizzo castration alone or in combination with ketoprofen (K), local anesthesia (LA), or caudal epidural anesthesia (EPI) on plasma cortisol, acute-phase proteins, interferon-gamma production, growth, and behavior of beef cattle, 50 Holstein x Friesian bulls (13 mo old, 307 +/- 5.3 kg) were assigned to (n = 10/treatment): 1) control (handled; C); 2) burdizzo castration (B); 3) B following K (3 mg/ kg of BW i.v.; BK); 4) B following LA (8 mL into each testis and 3 mL s.c. along the line where the jaws of the burdizzo were applied with 2% lidocaine HCl; BLA); and 5) B following EPI (0.05 mg/kg of BW of xylazine HCl and 0.4 mg/kg of BW of lidocaine HCl as caudal epidural; BEPI). The area under the cortisol curve against time was lower (P < 0.05) in BK than in B, BLA, or BEPI animals. On d 1 after treatment, plasma haptoglobin concentrations were higher (P < 0.05) in B, BLA, and BEPI than in BK animals. On d 3, haptoglobin and plasma fibrinogen concentrations were higher (P < 0.05) in all castration groups than in C. On d 7, haptoglobin and fibrinogen concentrations remained higher (P < 0.05) in BLA than in B and C animals. On d 1, concanavalin A-induced interferon-gamma production was lower (P < 0.05) in B, BLA, and BEPI than in C, but there was no difference between BK and C animals. From d -1 to 35, ADG was lower (P < 0.05) in B, BLA, and BEPI animals, but not in BK compared with C animals. Overall, there was a higher (P < 0.05) incidence of combined abnormal postures in B than in C, BK and BEPI animals. Although the use of K and EPI decreased (P < 0.05) these postures compared with B alone or B with LA, there was no difference between the K and EPI treatment. In conclusion, burdizzo castration increased plasma cortisol and acute-phase proteins, and suppressed immune function and growth rates. Local anesthesia prolonged the increase in acute-phase proteins. Ketoprofen was more effective than LA or EPI in decreasing cortisol and partially reversed the reduction in ADG following castration. The use of K or EPI was more effective than LA in decreasing pain-associated behavioral responses observed during the first 6 h after treatment. Systemic analgesia with ketoprofen, a non-steroidal antiinflammatory drug, was more effective in reducing inflammatory responses associated with castration than LA or EPI.  相似文献   

8.
This study explores the characteristics of Staphylococcus aureus (S. aureus) in swine and their human handlers in a convenience sample of 35 farms in Connecticut. Husbandry practices are clearly different from better‐known concentrated animal feeding operations (CAFOs) with less intensive rearing conditions. Nasal samples were collected from 263 pigs and nine humans on 35 farms during the 2010 rearing season. Samples were analysed using established microbiology methods, and resulting methicillin‐sensitive (MSSA) and resistant (MRSA) isolates were typed by pulsed‐field gel electrophoresis (PFGE) and spa typing. PCR was used to detect the presence of the Panton‐Valentine Leukocidin (PVL) gene, a cytotoxin usually associated with CA‐MRSA infection. A farm assessment form and questionnaire were used to obtain the information about husbandry practices and human exposure risk, respectively. Staphylococcus aureus colonized swine and humans were found in 51% (18/35) of the farms sampled at a rate of 30% (85/259) and 22% (2/9), respectively. Eight pigs and two humans were MRSA positive on five farms. MRSA in swine was related to healthcare‐associated (HA), community‐associated (CA) or livestock‐associated (LA) MRSA strains, whereas humans were colonized with HA‐MRSA. On the basis of spa typing, there was evidence of human–animal transmission thereby signifying humanosis/reverse zoonoses. The PVL gene was found in 88% (7/8) of MRSA swine isolates, the first time this gene has been seen in colonized pigs sampled on US farm. MSSA isolates belonged to six spa types: t337 (41%), t034 (12%), t334 (12%), t4529 (12%), t8760 (18%) and t1166 (6%) including LA strains. This is the first time spa type t8760 has been reported and the only MSSA with the PVL gene. In summary, MRSA including LA strains (LA‐MRSA) can be found on small farms with different husbandry practices from CAFOs, suggesting that preventive measures for zoonotic MRSA infection should address a range of animal production.  相似文献   

9.
The purpose of the present study was to determine the most effective time interval between the administration of sufentanil long acting (LA) and the induction of sevoflurane anaesthesia in dogs. The occurrence of sedation, analgesia and other marked side-effects were evaluated in addition to the possible dosage-reducing effect of sufentanil on sevoflurane in dogs. Forty clinically normal beagles aged 1-2 years and weighing between 8.4 and 13.6 kg were included. Two control groups were used: one group of dogs (A) received sufentanil LA (50 microg/kg i.m.) and a second group (B) the sufentanil vehicle followed by standard inhalation anaesthesia of 90 min. After premedication with sufentanil LA immediately before (C0), 15 min (D15) or 30 min (E30) prior to induction with thiopental (i.v.) the dogs were anaesthetized for 90 min with sevoflurane in oxygen. Pain and sedation scores were evaluated every 10 min during sevoflurane anaesthesia and at 2 (T120), 4 (T240) and 24 h (T1440) after initiation of anaesthesia. The occurrence of adverse reactions such as hypothermia, lateral recumbency, ataxia, noise sensitivity, vomiting, defaecation, salivation, nystagmus and excitation was observed at the same time-points. During the recovery period pain scores were lower and sedation scores higher in the sufentanil LA groups. In many dogs acceptable pain and sedation scores persisted during 24 h. Several dogs showed ataxia, lateral recumbency, arousal on auditory stimulation, defaecation, salivation and excitation at several time-points after sufentanil LA administration. Sufentanil LA in addition to sevoflurane anaesthesia offered beneficial dosage-reducing analgesic effects up to 69.8% for thiopental and 78.3% for sevoflurane; although several typical opioid side-effects occurred. To achieve this advantageous dosage-reducing effect 15 min should be respected between sufentanil LA administration and induction of sevoflurane anaesthesia.  相似文献   

10.
OBJECTIVE: To determine molecular characteristics, antimicrobial susceptibility, and toxigenicity of Clostridium difficile isolates from horses in an intensive care unit and evaluate associations among severity of clinical disease with specific strains of C difficile. DESIGN: Prospective study. ANIMALS: 130 horses. PROCEDURES: Feces were collected from horses admitted for acute gastrointestinal tract disease with loose feces and submitted for microbial culture and immunoassay for toxin production. Polymerase chain reaction assays were performed on isolates for toxins A and B genes and strain identification. RESULTS: Isolates were grouped into 3 strains (A, B, and C) on the basis of molecular banding patterns. Toxins A and B gene sequences were detected in 93%, 95%, and 73% of isolates of strains A, B, and C, respectively. Results of fecal immunoassays for toxin A were positive in 40%, 63%, and 16% of horses with strains A, B, and C, respectively. Isolates in strain B were resistant to metronidazole. Horses infected with strain B were 10 times as likely to have been treated with metronidazole prior to the onset of diarrhea as horses infected with other strains. Duration from onset of diarrhea to discharge (among survivors) was longer, systemic inflammatory response syndromes were more pronounced, and mortality rate was higher in horses infected with strain B than those infected with strains A and C combined. CONCLUSIONS AND CLINICAL RELEVANCE: Horses may be infected with a number of heterogeneous isolates of C difficile. Results indicated that toxigenicity and antimicrobial susceptibility of isolates vary and that metronidazole-resistant strains may be associated with severe disease.  相似文献   

11.
五种土霉素注射液在猪体内的药物代谢动力学比较研究   总被引：1，自引：1，他引：0  
对五种土霉素注射液产品(A、B、C、D和得米先20%)进行了猪体内的药代动力学比较研究。五组猪分别按20 mg/kg的剂量耳后肌肉注射,HPLC测定血药浓度,3P97软件拟合血药浓度-时间数据,计算药代动力学参数。五种长效土霉素注射液均符合二室开放模型,峰浓度值分别为1.511、.772、.132、.022、.72μg/mL,AUC分别为48.456、0.22、52.41、66.045、9.8(μg.h)/mL。比较而言,得米先20%药动学最好,其次为产品C,产品B最差。  相似文献   

12.
抗副鸡嗜血杆菌血清A和C型株所制备的两个血清型单克隆抗体（MAbs），分别对副鸡嗜血杆菌血清型A、B、C中的各型参考株作HI和dot－blotting试验。一种MAb（E5C12D10）为抗血清型A代表株221，另一种MAb（F2E6）为抗血清型C代表株S1。在两种试验中，不同血清型的MAbs可与对应的血清型中的副鸡嗜血杆菌株血凝（HA）抗原反应，而与血清型B代表株91、147均无反应。故这些MAbs可用于dot－blotting或HI试验进行副鸡嗜血杆菌定型。  相似文献   

13.
In this study, the association between virulence genotypes and phylogenetic groups among Escherichia (E.) coli isolates obtained from pet dogs and cats with cystitis was detected, and fingerprinting methods were used to explore the relationship among strains. Forty uropathogenic E. coli (UPEC) isolated from dogs (n = 30) and cats (n = 10) in Italy were analysed by polymerase chain reaction (PCR) for the presence of virulence factors and their classification into phylogenetic groups. The same strains were characterized by repetitive extragenic palindromic (REP)- and enterobacterial repetitive intergenic consensus (ERIC)-PCR techniques. We found a high number of virulence factors such as fimbriae A, S fimbriae (sfa) and cytotoxic necrotizing factor 1 (cnf1) significantly associated with phylogenetic group B2. We demonstrated a high correlation between α-hemolysin A and pyelonephritis C, sfa, and cnf1 operons, confirming the presence of pathogenicity islands in these strains. In addition, UPEC belonging to group B2 harboured a greater number of virulence factors than strains from phylogenetic groups A, B1, and D. REP- and ERIC-PCR grouped the UPEC isolates into two major clusters, the former grouping E. coli strains belonging to phylogenetic group B2 and D, the latter grouping those belonging to groups A and B1. Given the significant genetic variability among the UPEC strains found in our study, it can be hypothesized that no specific genotype is responsible for cystitis in cats or dogs.  相似文献   

14.
The increased prevalence of leishmaniasis in dogs in Mediterranean countries has necessitated use of a reliable specific method of immunodiagnosis. Research was carried out on 88 dogs, among which were 26 with severe clinical leishmaniasis (group A), 15 with mild signs of disease (group B), and 11 without signs of disease (group C); in addition, 15 were healthy (group D) and 21 were affected with various other diseases (group E). Dogs of groups D and E were used as controls. Infection was confirmed by cultural isolation of the parasite. Serotests that were used included the indirect immune fluorescent (IFAT) and indirect hemagglutination (IHAT) tests and counterimmunoelectrophoresis (CIEP); using these tests, specific antibodies against Leishmania infantum were detected in all 88 dogs. Sensitivity of the IFAT was 100% in dogs of groups A, B, and C; for the IHAT, it was 65.38%, 60%, and 54.5%, respectively, and for CIEP, it was 96.1%, 80%, and 72.7%, respectively. For dogs of groups D and E, specificity of the IFAT and IHAT was 100%, and for CIEP, it was 100% for group-D dogs and 90.5 for group-E dogs. Indirect immunofluorescence appeared to be the most specific and sensitive of the 3 tests.  相似文献   

15.
We produced monoclonal antibodies (mAbs) against bovine herpesvirus type 1 (BHV-1), Los Angeles strain, and then evaluated them as potential candidates for preparing diagnostic reagents. Of the 318 cell lines expressing antibodies to the virus, 60% (192) secreted IgG and 40% (126) secreted IgM. Twenty-six mAbs were selected based on enzyme-linked immunosorbent assay (ELISA) and virus neutralization (VN) titers and characterized by immunoprecipitation, immunofluorescence and immunoblots. The selected mAbs were assigned to one of four groups based on their immunoprecipitation patterns. Group A (4 mAbs) precipitated a complex of three glycoproteins with molecular weight (MW) 130 kDa, 72 kDa and 55 kDa, which presumably represented gI of BHV-1. Monoclonal antibodies of this group were highly reactive in ELISA but had low VN titers. Group B (4 mAbs) precipitated a glycoprotein with MW of 71 kDa (gIV). This group of mAbs had high VN titers. Group C (16 mAbs) precipitated a 97 kDa glycoprotein (gIII). Monoclonal antibodies of this group had high ELISA but low VN titers. Group D (2 mAbs) precipitated a double band of non-glycosylated proteins with MW of 37/32 kDa; these proteins could not be assigned to any of the antigens of BHV-1 previously described. ELISA and VN titers of this group of mAbs were low. To test the antigenic variability of the antigenic determinants which were recognized by these 4 groups of mAbs, we adapted Madin Darby bovine kidney cell-propagated BHV-1 Los Angeles strain to Crandell's feline kidney cell line. After the tenth passage in feline kidney cells, the epitopes on the 37/32 kDa peptide recognized by the mAbs group D were no longer detectable. Additional changes were noted in the electrophoretic mobility of the 130 kDa and 71 kDa glycoproteins (gI) identified by mAb of group A shifted downward. The 71 kDa glycoprotein (gIV) reactive with mAb group B and the 97 kDa (gIII) reactive with mAb group C remained stable. Since clone No. 191 of group B mAb was potent in ELISA, VN, immunoblots and immunofluorescence, and recognized an epitope which did not change under selective pressure, we feel that the mAb produced by this clone are a good candidate for the production of diagnostic reagents.  相似文献   

16.
Two new monoclonal antibodies (MAbs), D6D8D5 and B3E6F9, both directed against Haemophilus paragallinarum serovar C hemagglutinating (HA) antigen, were produced, and characteristics of the MAbs were compared with those of the previously described MAb F2E6 in dot-blot and hemagglutination-inhibition (HI) tests using two representative H. paragallinarum strains each of serovars A, B, and C strains and 55 Japanese serovar C field isolates. MAb D6D8D5 and MAb F2E6 reacted with all serovar C strains and field isolates in the dot-blot test. However, MAb D6D8D5 showed various degrees of inhibition of the HA activity of field isolates. In the enzyme-linked immunosorbent assay-competition test, MAb D6D8D5 did not compete with MAb F2E6. MAb B3E6F9 reacted with strain S1, serovar C but not with strain Modesto, serovar C in both dot-blot and HI tests. Three out of 55 field isolates did not react with MAb B3E6F9. Neither MAb reacted with the serovar A and B strains.  相似文献   

17.
猪多杀性巴氏杆菌的分离鉴定及生物学特性研究   总被引：17，自引：2，他引：17  
用PCR方法配合生化鉴定，从有肺炎症状猪的肺脏及进行性萎缩性鼻炎(Progressive atrophic rhinitis，PAR)症状猪的鼻拭子中分离出66株多杀性巴氏杆菌(Pasteurella multocida，Pm)。然后做了药敏试验，并用PCR方法对这66株Pm进行分型及毒素基因的检测，用豚鼠皮肤坏死试验及小鼠致死试验对产毒素多杀性巴氏杆菌(Toxigenie Pasteurella multocida，T^ Pm)进一步鉴定。结果显示PCR鉴定与生化鉴定Pm结果完全一致；PCR分型表明有46株为D型Pm，18株为A型：Pm，1株为B型Pm，1株无法定型；有8株用PcR检测为T^ Pm；豚鼠皮肤坏死试验及小鼠致死试验对这8株T^ Pm的进一步鉴定也表明均为产毒素菌株。所鉴定的8株T^ Pm都为D型，都分离于有严重PAR症状的猪。  相似文献   

18.
选择12头中国荷斯坦奶牛随机分为4组,采用4×4拉丁方试验设计,A组为对照组(基础日粮),B、C和D组分别在A组基础上添加120、280和380 mg/kg蛋氨酸锌,研究其对奶牛生产性能和相关生化指标的影响。结果表明:B、C和D组的日均泌乳量分别比A组提高了1.28kg(5.65%)、1.59 kg(7.02%)和1.66 kg(7.33%),均差异显著(P<0.05);4%标准乳量分别提高了0.98 kg(4.73%)、1.22 kg(5.89%)和1.16 kg(5.60%),均达差异显著水平(P<0.05);乳脂率、乳糖、非脂乳固形物含量均差异不显著(P>0.05);乳蛋白分别提高了4.56%、7.02%和7.72%,均差异显著(P<0.05);乳锌含量分别提高了1.05μg/mL(36.59%)、1.59μg/mL(55.40%)和2.08μg/mL(72.47%),均差异极显著(P<0.01);体细胞数分别降低了1.64×104/mL(5.65%)、3.23×104/mL(11.12%)和3.89×104/mL(13.39%),均差异显著(P<0.05);平均干物质采食量分别提高了0.19 kg(0.98%)、0.29 kg(1.51%)和0.32 kg(1.66%),均差异不显著(P>0.05)。经济效益分析表明,C组每牛日均比A组增收3.42元,最为理想。  相似文献   

19.
为探讨复合中草药制剂对热应激奶牛泌乳性能、抗氧化能力及免疫功能的影响,选择产奶中后期的健康中国荷斯坦奶牛20头,按泌乳时间和产奶量相近的原则均分为A、B、C和D 4组。A组为对照组,饲喂基础日粮;B、C和D组分别在每头牛的基础日粮中添加50,100和150 g/d水平的复合中草药制剂。每天测定产奶量,在试验的第1(添加复合中草药制剂之前)、20和40天逐头采集血样,测定不同剂量复合中草药制剂对热应激奶牛血淋巴细胞凋亡率、血液抗氧化酶GSH-Px、CAT基因及血淋巴细胞凋亡相关基因Bcl-2、p53表达水平的影响。结果表明,B、C和D组的日产奶量较对照组分别提高了20.26%(P<0.05),24.20%(P<0.05)和16.98%(P>0.05);乳中体细胞数较对照组分别降低了21.68%(P<0.05),29.88%(P<0.05)和24.22%(P<0.05);奶牛血液抗氧化酶GSH-Px、CAT基因的表达水平分别比对照组提高了5.00%(P>0.05),13.00%(P<0.05),6.00%(P>0.05)和5.00%(P>0.05),16.00%(P<0.05),3.00%(P>0.05);奶牛血淋巴细胞平均凋亡率分别比A组降低了18.20%(P>0.05),17.86%(P>0.05)和12.51%(P>0.05);奶牛血淋巴细胞Bcl-2在20,40 d时均极显著高于A组,全期的平均水平分别比对照组高出105.00%(P>0.05),113.00%(P>0.05)和57.00%(P>0.05);p53基因的表达水平在20,40 d时均极显著低于A组,全期的平均水平分别比对照组降低了24.00%(P>0.05),28.00%(P>0.05)和20.00%(P>0.05)。试验组中,C组奶牛的日产奶量、体细胞数、血液抗氧化酶活性、淋巴细胞凋亡相关基因表达等指标要优于B、D组;而血液淋巴细胞凋亡率指标为B组最好。说明复合中草药制剂能有效改善热应激奶牛的泌乳性能、抗氧化能力及免疫功能,且在本试验条件下,每头牛日粮中添加100 g/d水平的效果要优于50及150 g/d水平。  相似文献   

20.
日粮系酸力水平对羔羊生产性能和养分表观消化率的影响   总被引：1，自引：0，他引：1  
为了研究日粮不同系酸力水平对羔羊生产性能和饲料养分消化率的影响,将24只7日龄萨能奶山羊公羔随机分成4组,A组饲喂基础H粮,B、C和D 3个试验组分别饲喂在基础日粮中添加3.0、9.0和15.0 kg/t酸化剂的试验日粮(补饲料系酸力水平分别为30.0、20.0和10.0 mmol HCI/100 g;日粮系酸力水平分别为39.3、35.2和31.1 mmol HCl/100 g),研究日粮系酸力水平对早期断奶羔羊生产性能和养分表观消化率的影响.结果表明:(1)B、C和D组羔羊日增重极显著高于A组(P<0.01),C、D组显著高于B组(P<0.05);(2)饲料酸化可有效改善羔羊健康状况,B、C和D组羔羊腹泻率分别较A组降低了36.36%、28.65%和81.82%;(3)B,C和D组CP、GE表观消化率极显著高于A组(P<0.01),C、D组极显著高于B组(P<0.01);(4)B、C和D组DM表观消化率极显著高于A组(P<0.01),C、D组极显著高于B组(P<0.01),D组显著高于C组(P<0.05).结果提示,降低羔羊日粮系酸力水平,可以显著改善羔羊日增重、料重比和羔羊对日粮主要养分表观消化率,有效改善羔羊健康状况,在本研究条件下以补饲料系酸力水平10.0 mmol HCl/100 g即日粮系酸力水平31.1 mmol HCl/100 g组效果最好.  相似文献   

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