共查询到18条相似文献，搜索用时 312 毫秒
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应用单克隆抗体夹心酶联免疫吸附试验检测兔出血症病毒 总被引：3，自引：0，他引：3
应用建立的单克隆抗体夹心酶联兔疫吸附试验（ＭｃＡｂ－ＥＬＩＳＡ）,检测了人工感染兔出血症病毒（ＲＨＤＶ）ＤＪＲＫ细胞毒、肝毒的兔以及自然感染ＲＨＤＶ的兔的组织样品。结果表明,感染死亡兔的肝、脾、肾、骨髓样品病毒抗原的检出率为１００％,淋巴结和肌肉的检出率分别为９７．５％和７９．５％。ＭｃＡｂ－ＥＬＩＳＡ能检出肌肉中血凝试验不能检出的ＲＨＤＶ抗原。此外,还用ＭｃＡｂ－ＥＬＩＳＡ检测了肝毒人工感染兔血中ＲＨＤＶ的动态,并对１０份兔出血症脏器灭活苗的效价作了滴定。 相似文献
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兔出血症病毒接种幼年兔及乳兔的人工感染试验 总被引：3，自引：0，他引：3
用兔出血症病毒人工接种一月龄与二月龄幼年仔兔，结果证实兔出血症病毒亦能感染并致死幼年仔兔，但感染类型与青成年兔的典型兔出血症不同，主要特征有病程延长，血凝价低或无血凝和黄疸肝炎等等。用兔出血症病毒特异性单克隆抗体建立的ＥＬＩＳＡ试验及反向间接血凝试验自血凝试验阴性的幼年兔各脏器均能检测到兔出血症病毒。人工感染乳兔虽未致临床病症，但扑杀检查见有坏死性肝炎病变和轻度肾小球性肾炎，且自肝肾等血凝检测阴性的病科中，用ＥＬＩＳＡ试验及细胞培养均可检测和分离到病毒。研究结果表明，血凝阴性并不能排除非典型兔病毒性出血症感染。 相似文献
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应用免疫组化PAP法检测了人工感染成年患兔、30～40日龄患兔以及自然感染患兔体内兔出血症病毒（RHDV）抗原的动态分布。结果表明,在成年患兔的肝、肾、脾、胃、十二指肠、睾丸以及幼兔的肝、肾、脾中检出了RHDV抗原;无论在成年或幼龄患兔,RHDV抗原主要位于受侵害细胞胞浆中,少部分位于核中;在成年患兔,RHDV抗原阳性细胞的数量随病程发展而增加,而在幼龄患兔,这种增加趋势不明显。本文还分析了RHDV抗原在患兔体内的动态分布与病变形成之间的关系。 相似文献
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自人工感染阿留申病病毒(ADV)的水貂无菌采取组织、脏器,以超速离心法制备ADV抗原,应用对流免疫电泳检测表明,ADV抗原主要分布于淋巴结、脾、肝、小肠和胃中。从胃和小肠中能提出ADV抗原属首次报道。 相似文献
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刘源利 《河南畜牧兽医(综合版)》2015,36(1)
兔病毒性出血症,俗称兔瘟,是由兔病毒性出血症病毒引起的一种急性、烈性和高度接触性传染病.以呼吸系统充血、出血,肝、肾、脾以及消化道发生出血为主要症状.该病的发病率、死亡率极高,对养兔生产危害极大.
1 流行病学
该病自然感染条件下,只导致兔发病.病兔、病死兔、隐性感染带毒兔、带毒野兔以及其内脏器官、附属物、排泄物等是该病的传染源. 相似文献
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双抗体夹心ELISA检测伪狂犬病病毒的研究 总被引：11，自引：0，他引：11
建立了检测伪狂犬病病毒（ＰＲＶ）抗原的双抗体夹心ＥＬＩＳＡ,试验方法的最佳工作条件为：抗体包被量为５μｇ／孔,酶标抗体的浓度为１：２００。对人工感染兔和自然感染猪的组织脏器检测,结果发现扁桃体,脑和肺的检出率最高,其次为心,肝,脾,肾等组织。 相似文献
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采用抗兔出血症病毒的单克隆抗体对来自全国不同地区的12株兔出血症病毒分别进行了血凝抑制(HI)试验,HI 效价达1:100×2~7～1:100×2~9.结果说明,该单抗对这12株病毒都能抑制其血凝现象.应用该单抗制备的荧光抗体检测了兔出血症病毒在感染兔体内主要脏器组织的分布动态。结果提示,该病毒在细胞核内增殖。经消化道感染兔时,病毒首先在入侵局部肠道粘膜内增殖。然后再侵入肝脏和脾脏。大量病毒生成后,又侵入其它的器官组织,病兔处于病毒血症状态。 相似文献
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兔出血症三种快速诊断法比较研究 总被引：5，自引：0，他引：5
本试验对快速诊断兔出血症(RHD)的免疫酶染色法(IES),斑点酶联免疫吸附试验(Dot-ELISA)与间接荧光染色法(IFS)进行了比较。试验表明,三种快速诊断法对17份实验感染兔肝材料的检测结果与血凝试验(HA)的阳性符合率分别为88.2％、88.2％及93.3％,阴性符合率均为100％;对4份脏器材料检测结果证明,兔出血症病毒(RHDV)含量依次为;肝>脾>肾>肺>心肌。肝触片及快诊膜分别在4℃和室温放置1个月后,其检测结果不受影响。三种快速诊断对RHDV的检测具有很高的特异性、敏感性和稳定性。Dot-ELISA具有经济、简便、快速、敏感、准确的优点。 相似文献
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对送检的9只家兔进行病理解剖,对心血、肝、脾及肠系膜淋巴结进行细菌分离培养,并选取心、肝、脾、肺等组织按常规病理制片进行病理组织学检查,应用免疫组化染色(ABC法)检测肝细胞内兔病毒性出血症病毒(RHDV)抗原,结果证实此次家兔自然暴发的传染病即是兔病毒性出血症(RHD)。其临床主要表现为最急性型及急性型,病理变化特点以全身微循环障碍为主;DIC形成,以实质器官淤血、水肿、出血、变性、坏死为主要特点,其中以肝、肾、脾等器官病变较重,尤其肝脏的病变具有特征性。综合病理学检测结果,确定此次家兔暴发的是RHD。 相似文献
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Outbreak of rabbit hemorrhagic disease in domestic lagomorphs 总被引：2，自引：0，他引：2
Campagnolo ER Ernst MJ Berninger ML Gregg DA Shumaker TJ Boghossian AM 《Journal of the American Veterinary Medical Association》2003,223(8):1151-5, 1128
Rabbit hemorrhagic disease (RHD) was diagnosed in domestic lagomorphs on a rabbit farm in Illinois. Clinical signs of RHD in affected rabbits included signs of depression, anorexia, fever, paddling, convulsions, and sudden death. Findings of necropsies and histologic evaluations of specimens of liver and spleen were indicative of RHD. In liver specimens obtained from dead rabbits, RHD viral antigen was detected via hemagglutination assay and viral antigen-detection ELISA. The source of the outbreak was traced to a rabbitry in Utah. As the disease spread, the outbreak involved rabbits in various regions of the United States; > 4,800 rabbits were euthanatized and buried as a result of the depopulation effort in several states. The economic impact of the disease can be considerable; if the disease is suspected, it is imperative that the appropriate state or federal veterinarian's office be contacted immediately. 相似文献
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Previous studies have shown that feral cats (Felis catus) from rabbit haemorrhagic disease (RHD) epidemic areas in New Zealand had antibodies against RHD Virus (RHDV) and RHDV RNA was identified by nested RT-PCR from one seropositive feral cat liver. To assess whether RHDV replicates and produces clinical consequences in cats following the consumption of RHDV-infected rabbit, a challenge trial was conducted by feeding cats RHDV-infected rabbit livers. Antibodies against RHDV were detected by immunoassay from sera of cats collected 10 days after the consumption of RHDV-infected livers. Animals fed four times with RHDV-infected livers, had higher antibody titres than animals fed only once. RHDV RNA was detected by nested RT-PCR from mesenteric lymph nodes, tonsil, spleen and liver of cats fed with RHDV-infected livers. RHDV anti-genomic RNA was also detected by nested RT-PCR from mesenteric lymph nodes collected from one animal 2 days after the fourth feed. RHDV was detected by antigen ELISA from cat faeces 1-2 days after the consumption of RHDV-infected livers. Even though a large amount of RHDV has been used, cats did not show any signs of disease. Although abortive RHDV replication could not be ruled out, active RHDV replication was not demonstrated. 相似文献
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Detection of rabbit haemorrhagic disease virus antigen in tissues by immunohistochemistry 总被引：1，自引：0，他引：1
Formalin fixed liver, spleen, kidney, heart, lung, duodenum and appendix tissues from nine rabbits, experimentally infected with rabbit haemorrhagic disease virus (RHDV), were investigated for evidence of RHDV antigen by the direct avidin-biotin peroxidase complex immunohistochemical method. In all the rabbits examined, RHDV antigen was detected in degenerative and necrotic hepatocytes of the liver tissues. The area involved coincided with histopathological lesions on serial liver sections. The RHDV antigen was expressed in the cytoplasm of the hepatocytes, suggesting that RHDV replicated in these cells. RHDV antigen was also detected in the spleen. The results of immunohistochemistry were supported by the demonstration of RHDV protein by Western blot analysis and of RHDV particles by protein A-gold immunoelectron microscopy in the liver homogenate from all the rabbits that were examined. 相似文献
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猪瘟活疫苗(脾淋源)污染外源性兔出血症病毒的检测与启示 总被引：1，自引：1，他引：0
在进行2批猪瘟活疫苗（脾淋源）效力检验时,出现效力检验家兔突然死亡现象,为了查明家兔死亡原因,采用无菌检验、支原体检验、血凝试验、兔体中和试验、酶联免疫吸附试验（ELISA）对疫苗或注苗后死亡家兔肝脏、脾脏混合病料进行了检测。结果显示,疫苗的无菌检验、支原体检验结果均为阴性;疫苗及注射疫苗死亡家兔肝脏、脾脏混合病料具有较低的血凝价,血凝试验结果均为可疑;在兔体中和试验中,中和组家兔2／2健康存活,未中和的疫苗对照组家兔2／2死亡;疫苗及注射疫苗死亡后家兔肝脏、脾脏混合病料的ELISA检测结果均为阳性。检测结果证实疫苗中含有兔出血症病毒（Rabbit Haemorrhagic Disease Virus,RHDV）。猪瘟活疫苗（脾淋源）污染RHDV的现象启示：应该加强猪瘟活疫苗（脾淋源）抗原制备过程的控制;同时有必要对猪瘟活疫苗（脾淋源）质量标准进行修订使之进一步补充完善。 相似文献
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J H Park H Kida K Ueda K Ochiai M Goryo C Itakura 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(10):749-754
Causative agent of rabbit haemorrhagic disease (RHD) was purified by CsCl density gradient centrifugation from the liver homogenate of rabbits infected with RHD virus which originated from Korea. The viral particles were 35-40 nm in diameter, and had hollow depressions on their surface. Protein A-gold immunoelectron microscopy clearly showed that the convalescent antisera of diseased rabbits reacted specifically with the virus particles. SDS-PAGE and Western blot analyses demonstrated that the structural protein of the virus was composed of a single major polypeptide of 63 kD. These findings indicate that the causative agent of RHD, tentatively named as picornavirus in Korea, belongs to calicivirus. 相似文献
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N Stoercklé-Berger B Keller-Berger M Ackermann F Ehrensperger 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(4):237-245
In the present study the diagnostic use of a biotinylated serum from an immune rabbit was investigated by means of an Avidin-Biotin-Complex (ABC)-Peroxidase method on paraffin sections. 15 cases of RHD which had been verified histologically and/or by haemagglutination test (HA), 4 suspected cases and 3 cases without history of RHD were included (cases 1 to 22). From 5 prospective cases a wider tissue range was examined (cases 23 to 25 and 29 to 30). Furthermore lungs, liver and placenta of 3 fetuses from a RHD affected dam were investigated (cases 26 to 28). The 20 typical cases had intense intranuclear and diffuse intracytoplasmic immunostaining of hepatocytes, predominantly in the periportal areas. In some cases there was also positive staining of macrophages in the lungs (4 cases), spleen (4 cases) and in lymph nodes (1 case). Positive granular staining in the renal mesangial cells of the glomeruli was observed in 1 case. No positive staining was observed in the 3 negative cases. In contrast to other reports (4), crossreactivity of these antigens to Porcine Parvovirus (PPV) could not be confirmed. Furthermore the RHD virus (RHDV) seems not to crossreact with Feline and Bovine Parvoviruses. 相似文献