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1.
为做好河南省小反刍兽疫防控工作,全面掌握小反刍兽疫免疫抗体水平及病毒感染状况,按照2020年河南省动物疫病专项监测方案要求,在全省开展了小反刍兽疫专项监测。在群内采用随机采样原则,对全省所有种羊场以及较大规模羊场（存栏量≥3 000只）进行调查,共监测60个养殖场,采集血清学样品1 817份,病原学样品1 718份,使用竞争ELISA、荧光RT-PCR分别检测小反刍兽疫免疫抗体水平及病毒核酸。结果显示：小反刍兽疫平均免疫抗体合格率为73.80%,其中商品场免疫抗体水平优于种羊场;不同企业生产的小反刍兽活疫苗免疫抗体合格率均高于70%的农业农村部最低标准;不同区域羊场免疫抗体水平有所差异,免疫2次及以上的羊场免疫抗体水平优于只免疫1次的;所有样品小反刍兽疫病毒核酸检测均为阴性。结果表明,河南省近期发生小反刍兽疫疫情的风险较低,防控成效较好。 相似文献
2.
为切实做好云南省小反刍兽疫消灭工作,保障羊产业稳定健康发展,依据《国家中长期动物疫病防治规划(2012—2020年)》和《全国小反刍兽疫消灭计划(2016—2020年)》要求,2016—2019年分别采用ELISA、RT-PCR方法,在昆明市开展了小反刍兽疫免疫抗体和病毒核酸检测。结果显示:909个集中监测养殖场点的8972份血清样品中,检出免疫合格场点841个,平均场点免疫合格率为92.5%;检出免疫合格样品7405份,平均个体免疫合格率为82.53%,虽然超过了农业农村部要求的不低于70%的标准,但各年度的个体合格率有下降趋势;规模场和散养户的个体免疫合格率分别为84.97%和82.07%,场点合格率分别为83.33%和93.38%,均无显著差异(P>0.05);不同县(市、区)的个体免疫合格率为77.36~93.17%,场点合格率为83.33%~98.55%,差异均不显著(P>0.05);在3812份羊眼(鼻)棉拭子和组织样品中检出疑似核酸阳性2份,分别来自2个散养户。结果表明:昆明市小反刍兽疫整体防控效果较好,发生大面积流行的风险较低,但仍有散发疫情风险。因此,仍需继续做好小反刍兽疫的强制免疫、监测和流行病学调查工作,同时加强羊只的引种检疫和流通监管,最终到达消灭小反刍兽疫的目标。 相似文献
3.
为落实国家小反刍兽疫消灭计划,掌握安徽省小反刍兽疫流行趋势与免疫状况,2017—2019年,每年从安徽省16个地市、2个直管县的种羊场、商品代羊场和散养户抽取不同数量的羊血清和鼻拭子样品,分别用竞争ELISA、荧光RT-PCR方法检测小反刍兽疫免疫抗体与病毒核酸。3年共抽样检测842个场点的15 943份血清学样品,平均免疫个体合格率为88.16%、场点合格率为89.31%,每年的个体合格率和场点合格率均在84%以上;检测12 411份病原学样品,未检测出阳性样品。但芜湖市3年的平均个体免疫合格率仅为68.24%,淮南市平均场点合格率仅为65.28%,未达到农业农村部要求的70%以上的标准。结果表明,安徽省小反刍兽疫整体免疫效果较好,疫情暴发风险较低,基本达到了免疫无疫标准,但仍要关注免疫效果较差地区的免疫、监测与流行病学调查,加大调运监管力度,降低疫情发生风险。 相似文献
4.
为掌握山西省小反刍兽疫免疫状况及其流行情况,推动实施小反刍兽疫消灭计划,2017—2019年在全省11个地市,采用配额抽样方法,采集种羊场、商品代羊场、羊散养户共4 038份血清学样品和8 397份病原学样品,通过竞争ELISA、荧光定量PCR方法分别进行免疫抗体和病毒核酸检测。结果显示：2017—2019年全省小反刍兽疫免疫抗体个体合格率分别为83.51%、81.62%、84.15%,场群合格率分别为84.91%、81.20%、76.25%,呈下降趋势;病原学个体阳性率分别为0、0.08%、0.15%,群体阳性率分别为0、1.63%、1.25%,呈上升趋势。商品代羊场个体合格率为82.25%,低于种羊场（100%）和羊散养户（84.55%）,且有病原阳性检出（0.09%）。10个地市的场群合格率均在70%以上,但有2个地市刚达标准线,1个地市仅为60.00%,3个地市检出病原学阳性。结果表明,山西省小反刍兽疫整体防控效果较好,但有下滑趋势,且商品代羊场存在一定的病毒感染,部分地区免疫效果不佳,因而存在疫情发生风险。在目前暂不具备退出强制免疫的条件下,仍需加强免疫、监测和调运监管,力争尽快实现消灭小反刍兽疫的目标。 相似文献
5.
为降低河南省家禽新城疫传播风险,推动家禽养殖业健康安全发展,为新城疫综合防控提供科学依据,按照《河南省2022年动物疫病监测与流行病学调查计划》要求,分阶段采集家禽血清学和病原学样品,采用HI试验和荧光RT-PCR方法进行新城疫专项监测,监测新城疫血清抗体和病毒核酸。结果显示,河南省新城疫抗体场群合格率和个体合格率均在97%以上,远超出国家70%免疫合格率的标准,检出3个场点40份病毒核酸阳性样品,场群阳性率为2.40%,个体阳性率为1.09%。结果表明：河南省新城疫整体防控效果较好,免疫抗体水平较高,建立了良好的免疫屏障,但检出少数病毒核酸阳性样品,感染风险仍存在,需要做好定期监测和流行病学调查,及时补免、补防和消除隐患。 相似文献
6.
为了解河南省长垣市奶山羊小反刍兽疫疫情流行情况和免疫抗体水平,2018—2020年,采用阻断酶联免疫吸附剂测定(ELISA)法和实时荧光聚合酶链式反应(PCR)法,对长垣市32家奶山羊散养户采集羊血清和鼻拭子样品,进行羊小反刍兽疫免疫抗体及病原学检测。结果表明,2018年、2019年和2020年的血清抗体合格率分别为83.13%、85.64%和86.67%,平均85.17%,高于国家规定的70.00%。病原学监测全部样品为阴性。提示长垣市总体疫苗免疫效果良好,羊群具有抵抗小反刍兽疫侵袭的基本能力。为长垣市制定科学合理的防控措施、逐步消灭小反刍兽疫和退出小反刍兽疫免疫计划提供参考依据。 相似文献
7.
为了做好小反刍兽疫的防控工作,对河北省小反刍兽疫免疫效果进行了监测,于2015年选取河北省5个免疫区(即5个地市),每区选7个养殖场,每个养殖场检测30份样本进行免疫抗体检测;于2014—2016年连续3年监测衡水市某羊场小反刍兽疫的免疫效果,每年采集样本30份进行免疫抗体检测。结果表明:2015年不同地市养殖场的免疫合格率达标情况不同,5个地市小反刍兽疫抗体合格率依次为57%、57%、14%、71%、71%,地市3免疫合格率达到要求的养殖场数量最少;连续3年监测衡水市某羊场小反刍兽疫免疫抗体合格率均达到70%以上。 相似文献
8.
为了准确评估黔东南州2017～2019年小反刍兽疫(PPR)防控效果,本实验室对黔东南州16个县(市)进行小反刍兽疫免疫抗体和病原学监测。结果表明:2017～2019年共采集5 358份羊血清抗体总合格率为81.8%,超过农业部最低标准(70%);3 740份羊眼鼻拭子病毒核酸全部为阴性,无野毒感染。规模场免疫抗体合格率最高,达88.6%;散养户合格率为80.6%;屠宰场合格率最低,为76.9%。通过对我州羊群PPR疫苗免疫抗体和病原学监测,可以看到我州小反刍兽疫的免疫效果总体情况良好,为本病的防控提供预警预报信息和防控工作指导。 相似文献
9.
为了解河南省长垣市奶山羊小反刍兽疫疫情流行情况和免疫抗体水平,2018—2020年,采用阻断酶联免疫吸附剂测定（ELISA）法和实时荧光聚合酶链式反应（PCR）法,对长垣市32 家奶山羊散养户采集羊血清和鼻拭子样品,进行羊小反刍兽疫免疫抗体及病原学检测。结果表明,2018年、2019年和2020年的血清抗体合格率分别为83.13%、85.64%和86.67%,平均85.17%,高于国家规定的70.00%。病原学监测全部样品为阴性。提示长垣市总体疫苗免疫效果良好,羊群具有抵抗小反刍兽疫侵袭的基本能力。为长垣市制定科学合理的防控措施、逐步消灭小反刍兽疫和退出小反刍兽疫免疫计划提供参考依据。 相似文献
10.
从2015年开始,河池市对小反刍兽疫免疫抗体和病原进行监测,样品主要来自全市11个县(区)定点监测与日常监测,每年春秋两防检查采集,以期了解河池市小反刍兽疫免疫抗体和流行情况,科学评估免疫效果和小反刍兽疫发生的风险。监测结果显示:2017-2020年小反刍兽疫免疫抗体合格率分别为84.1%、90.4%、93.8%、95.5%,4年均达到农业农村部规定的70%以上的要求;病原分别监测1095、1139、886、650份,结果均为阴性。 相似文献
11.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
13.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
14.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
15.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
17.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
18.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
19.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
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