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产肠毒素性大肠杆菌与仔猪腹泻的致病过程密切相关。卵黄抗体具有价格便宜、制作简单等优点,能降低腹泻的发生率和严重程度,是防治仔猪大肠杆菌性腹泻的一种新方法。本文就国内外鸡抗猪产肠毒素性大肠杆菌卵黄抗体的应用作了简要介绍。 相似文献
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李清艳 《河南畜牧兽医(综合版)》2004,25(6):9-10
仔猪大肠杆菌性腹泻主要是由产肠毒素性大肠杆菌(EnterotoxigenicEscherichiacoil,ETEC)引起的仔猪的一种常见病,严重影响养猪业的发展。目前对该病的防治除加强综合性预防措施以外,生产上多采用ETEC的多价疫苗和基因工程苗免疫母猪,通过提高初乳中的抗体水平,来预防哺乳仔猪产肠毒素大肠杆菌性腹泻。然而母乳中的抗体保护作用是有限的和暂时的,在断奶后短期内将消失。应用高免血清治疗该病也是一种极为有效的方法,但血清价格昂贵,且易导致过敏反应。因此应用抗ETEC卵黄抗体治疗和预防新生和断奶仔猪腹泻的研究在国内外一直倍受瞩目。… 相似文献
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卵黄抗体被动免疫防治仔猪产肠毒素大肠杆菌性腹泻 总被引：3，自引：0，他引：3
用ETECK88+菌株C83907对120日龄待开产蛋鸡进行免疫,获得高免卵黄抗体粉。选用3日龄和21日龄的杜×长×大三元杂交早期断奶仔猪,人工感染C83907大肠杆菌,口服含抗产肠毒素大肠杆菌抗体的卵黄粉进行被动免疫。结果显示,3日龄仔猪服用抗ETEC卵黄抗体粉后,72h腹泻停止,而服用普通蛋黄粉组却仍然腹泻并且有66.7%的死亡率;服用抗ETEC卵黄抗体粉的21日龄仔猪仅有短暂腹泻现象,存活率100%,试验期间总体质量有所增加,而对照组表现严重腹泻伴有脱水现象,试验期间有部分猪只死亡;临床应用表明,喂以3g和5g抗ETEC卵黄粉组的治愈率与抗生素治疗组相当。研究结果表明,卵黄抗体对早期断奶仔猪有一定的促进生长和防止腹泻的效果。 相似文献
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仔猪腹泻尤其是断奶仔猪的腹泻是造成养猪业损失的主要原因之一，而集约化生产中仔猪大肠杆菌病发病率占新生仔猪胃肠病的50％以上（于力等，1998）。目前，防治仔猪大肠杆菌感染性腹泻的较有效手段是应用抗生素，但随着社会公众对食品安全要求的提高，寻找新的安全有效手段来防治仔猪腹泻已刻不容缓。本文综述了卵黄抗体在仔猪大肠杆菌性腹泻中的应用及其机理研究进展。 相似文献
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仔猪腹泻尤其是断奶仔猪的腹泻是造成养猪业损失的主要原因之一，而集约化生产中仔猪大肠杆菌病发病率占新生仔猪胃肠病的50％以上(于力等，1998)。目前防治仔猪大肠杆菌感染性腹泻的较有效手段是应用抗生素，但随着社会公众对食品安全要求的提高，寻找新的安全有效手段来防治仔猪腹泻已刻不容缓。本文综述了卵黄抗体在仔猪大肠杆菌性腹泻中的应用及其机理研究进展。 相似文献
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特异性卵黄抗体在防治仔猪大肠杆菌性腹泻中的应用 总被引：2，自引：0，他引：2
仔猪大肠杆菌性腹泻是由猪致病性大肠杆菌引起的一种常见传染病 ,具有发病急、传播快、死亡率高等特点 ,严重危害养猪业。产肠毒素大肠杆菌 (EnterotoxigenicEscherichiacoli,ETEC)是引起新生和断奶仔猪腹泻的主要致病菌之一 (Yokoya ma等 ,1 992 )。长期以来 ,该病的防治主要依赖于抗菌药物、大肠杆菌多价苗和基因工程疫苗。然而 ,抗生素在动物体内会导致药物残留和产生抗药性 ;用疫苗免疫母猪 ,虽可提高母乳中抗体含量 ,但母源抗体持续时间短 ,不足以保护仔猪整个生长阶段不致病 ;口服高免动物血清虽能有效防治该病 ,但成本较高很难在… 相似文献
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仔猪大肠杆菌性腹泻主要是由产肠毒素性大肠杆菌(Enterotoxigenic Escherichia coli,ETEC)引起的仔猪的一种常见病,严重影响养猪业的发展。目前对该病的防治除加强综合性预防措施以外,生产上多采用ETEC的多价疫苗和基因工程苗免疫母猪,提高初乳中的抗体水平,以防治哺乳仔猪产肠毒素大肠杆菌性腹泻。然而母乳中的抗体 相似文献
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Harmsen MM van Solt CB Hoogendoorn A van Zijderveld FG Niewold TA van der Meulen J 《Veterinary microbiology》2005,111(1-2):89-98
Oral administration of polyclonal antibodies directed against enterotoxigenic Escherichia coli (ETEC) F4 fimbriae is used to protect against piglet post-weaning diarrhoea. For cost reasons, we aim to replace these polyclonal antibodies by recombinant llama single-domain antibody fragments (VHHs) that can be produced efficiently in microorganisms. Six F4 fimbriae specific VHHs were isolated. The VHH that was produced at the highest level by yeast, K609, was further analysed. 3.8 mg/L K609 inhibited 90% of bacterial attachment to intestinal brush borders in vitro. Perfusion of a jejunal segment with at least 4 mg/L K609 reduced the ETEC-induced fluid loss, but only to 30%. Preventive administration of a high K609 dose (150 mg/(piglet day)) to piglets that were challenge infected with ETEC resulted in less severe diarrhoea only at 4 and 5 days post-infection, but did not improve average daily weight gain, ETEC shedding and piglet survival. Thus, we have shown that an antibody fragment that effectively inhibited in vitro ETEC adhesion to intestinal brush borders poorly protected piglets against experimental ETEC infection. 相似文献
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Snoeck V Huyghebaert N Cox E Vermeire A Vancaeneghem S Remon JP Goddeeris BM 《Veterinary immunology and immunopathology》2003,96(3-4):219-227
To prevent enterotoxigenic Escherichia coli (ETEC) induced postweaning diarrhoea, the piglet needs an active mucosal immunity at the moment of weaning. In the present study, the feasibility of oral vaccination of suckling piglets against F4+ETEC infection with F4 fimbriae was studied. Furthermore, oral vaccination with enteric-coated pellets of F4 fimbriae was compared to vaccination with F4 fimbriae in solution. Therefore, piglets were orally administered 1mg F4 fimbriae in pellets or in solution during three successive days at the age of 7 and 21 days, whereas control piglets were not vaccinated. Five days postweaning (33 days of age), all animals were orally challenged with F4+ETEC. Despite the induction of an immune response upon oral administration of both F4 fimbriae in pellets as in solution, the colonisation of the small intestine by F4+ETEC upon oral challenge could not be prevented. However, a marginal but significant reduction in F4+ E. coli faecal excretion was found in the piglets vaccinated with F4 fimbriae in pellets, indicating that the use of an enteric-coat which protects the F4 fimbriae against inactivation by milk factors and degradation by enzymes and bile improves vaccination. 相似文献
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Niewold TA van Dijk AJ Geenen PL Roodink H Margry R van der Meulen J 《Veterinary microbiology》2007,124(3-4):362-369
In order to establish the mechanism of spray dried plasma powder (SDPP) in improving pig health and performance, a diet containing either 8% SDPP, spray dried immune plasma powder (SDIPP), or control protein (soybean and whey) ration was fed to piglets in an experimental model of enterotoxigenic Escherichia coli F4 (ETEC) post-weaning diarrhoea (PWD). SDIPP was obtained from pigs immunized with a vaccine containing ETEC fimbrial subunit F4 and heat-labile toxin (LT), and SDPP from non-immunized controls. Average daily growth (ADG) was determined, and daily samples of rectal faeces were assessed for diarrhoea (as percentage of dry matter), and ETEC excretion (in CFU/g). SDPP and SDIPP significantly (p<0.05) reduced diarrhoea, and SDIPP significantly reduced ETEC excretion. ADG was not significantly (p>0.05) affected. After the experiment, 30% of piglets tested F4 receptor positive (F4R+). A significant correlation between F4R status and morbidity was found. In F4R+ animals, SDIPP significantly improved diarrhoea and ADG, and decreased ETEC excretion, and SDPP significantly improved diarrhoea and ADG. Surprisingly, SDPP reduced diarrhoea in F4R+ animals without significant reduction of ETEC excretion, which is most likely related to the presence of anti-LT antibodies in SDPP. The results show that oral protection against ETEC by SDPP is attributable to spontaneous antibodies, in this case anti-LT antibodies. Furthermore, the results indicate that the combination of anti-LT and anti-F4 antibodies as in SDIPP is most effective in ETEC prevention. Finally, the F4R distribution in the herd should be taken into account to correctly assess efficacy. 相似文献
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Bruins MJ Vente-Spreeuwenberg MA Smits CH Frenken LG 《Journal of animal physiology and animal nutrition》2011,95(3):388-398
Enterotoxigenic Escherichia coli (ETEC) is a main cause of diarrhoea in humans and piglets. In vitro, black tea extract (BTE) has anti-pathogenic properties. Anti-diarrhoeal properties of BTE were assessed in a pig model of gastrointestinal infection. At weaning (day 0), piglets (n = 96) were randomly assigned to a diet containing 0% (control), 0.4% or 0.8% (wt/wt) BTE during 27 days. Piglets were orally infected with 6.4 × 10(6) cfu of ETEC on day 6. Faecal consistency, feed intake and body weight were measured. In a sub-study (n = 30 piglets), the effect of BTE palatability on feed intake was assessed. Additionally, the effect of BTE on ETEC growth in the presence or absence of iron was studied in vitro. The 0.8% BTE diet reduced diarrhoea prevalence by 20% but also decreased feed intake by 16% and feed efficiency by 12% over the total period. The 0.4% BTE diet decreased feed efficiency and weight gain from day 13 onwards. The palatability study demonstrated that piglets preferred the control to the BTE diets. In vitro, BTE delayed ETEC exponential growth, which was reversed by iron addition. Although BTE had anti-diarrhoeal properties, this effect was accompanied by impaired performance. The absence of a correlation between diarrhoea prevalence and feed intake suggests that reduced diarrhoea directly results from BTE rather than from reduced feed intake caused by BTE astringency. 相似文献
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Influence of porcine intestinal pH and gastric digestion on antigenicity of F4 fimbriae for oral immunisation 总被引：1，自引：0，他引：1
Newly weaned piglets can be orally immunised against F4+ enterotoxigenic Escherichia coli (ETEC) infection with F4 fimbriae. However, to efficiently develop a vaccine against ETEC induced postweaning diarrhoea, knowledge of the stability of the F4 fimbriae to different pH and gastric digestion is needed. The gastrointestinal pH in suckling and recently weaned piglets was measured and the stability of F4 fimbriae to different pH and to pepsin was assessed in vitro. In the stomach the lowest pH was found in the fundus gland region. Gastric pH values below 2.5 were not found in suckling piglets or at the day of weaning, in contrast to piglets 1 and 2 weeks postweaning. Along the first half of the small intestine and in the caecum, a negative correlation was found between pH and age. The F4 fimbriae were stable to pH 1.5 and 2 for 2 h, whereas longer incubation periods resulted in conversion of the multimeric forms into monomers. The F4 fimbriae were partially degraded by incubation for 15-30 min in simulated gastric fluid at pH 1.5 and 2, and completely digested from 3 h onwards. At pH 3, the fimbriae maintained their antigenicity for at least 4h. The results demonstrate that gastric digestion will only have a limited impact on oral immunisation since liquid passes through the stomach relatively quickly (50% within 2 h). However, we previously demonstrated that the transit times are prolonged shortly after weaning. Shortly after weaning it could be necessary to protect the F4 fimbriae against gastric digestion to obtain efficient oral immunisation of the piglets. 相似文献
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Ut V Nguyen Vesna Melkebeek Bert Devriendt Tiphanie Goetstouwers Mario Van Poucke Luc Peelman Bruno M Goddeeris Eric Cox 《Veterinary research》2015,46(1)
F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by an F4-specific serum and intestinal IgA response. However, successful oral immunization of pigs with F4 fimbriae in the presence of maternal immunity has not been demonstrated yet. In the present study we aimed to evaluate the effect of maternal immunity on the induction of a systemic immune response upon oral immunization of piglets. Whereas F4-specific IgG and IgA could be induced by oral immunization of pigs without maternal antibodies and by intramuscular immunization of pigs with maternal antibodies, no such response was seen in the orally immunized animals with maternal antibodies. Since maternal antibodies can mask an antibody response, we also looked by ELIspot assays for circulating F4-specific antibody secreting cells (ASCs). Enumerating the F4-specific ASCs within the circulating peripheral blood mononuclear cells, and the number of F4-specific IgA ASCs within the circulating IgA+ B-cells revealed an F4-specific immune response in the orally immunized animals with maternal antibodies. Interestingly, results suggest a more robust IgA booster response by oral immunization of pigs with than without maternal antibodies. These results demonstrate that oral immunization of piglets with F4-specific maternal antibodies is feasible and that these maternal antibodies seem to enhance the secondary systemic immune response. Furthermore, our ELIspot assay on enriched IgA+ B-cells could be used as a screening procedure to optimize mucosal immunization protocols in pigs with maternal immunity. 相似文献