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四川省某肉牛场新引进的犊牛出现发热、咳嗽、呼吸困难、流鼻涕等呼吸道症状,为了解该病的病因,本试验采集了10份病牛深部鼻腔棉拭子样本,采用PCR方法检测了牛冠状病毒（BCoV）、牛病毒性腹泻病毒（BVDV）、牛传染性鼻气管炎病毒（IBRV）、牛腺病毒3型（BAV-3）、牛支原体（M.Bovis）、多杀性巴氏杆菌（P.multocida）、溶血性曼氏杆菌（M.haemolytica）及睡眠嗜组织菌（H.somni）等8种常见呼吸道病原。结果显示：BCoV检出率为70%,BVDV检出率为30%,其余病原均未检出,表明该肉牛场犊牛的呼吸道疾病是由BCoV和BVDV混合感染引起的。 相似文献
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牛呼吸道疾病是牛最常见的一类传染病,发病快、传染快,对养牛业的影响比较大。本文就牛支原体、牛巴氏杆菌病、牛呼吸道合胞体病毒病、牛传染性鼻气管炎、牛病毒性腹泻/黏膜病的认识和防治进行了简单的论述,以期为养殖者提供参考。 相似文献
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牛呼吸系统疾病的发生给养牛业带来严重的经济损失,阻碍了我国牛业的发展。本文分析了牛呼吸系统疾病中常见的牛呼吸道合胞体病毒感染、牛传染性鼻气管炎、牛病毒性腹泻病毒感染、牛副流行性感冒等4种病毒性疾病和牛支原体肺炎、牛溶血性曼氏杆菌感染、牛多杀性巴氏杆菌病等3种细菌性疾病的发生原因、特点及相关症状,并概述了这7种呼吸系统疾病的主要防控措施及当前的研究进展,以期为该类疾病的综合防治提供参考。 相似文献
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范俊 《北方牧业(奶牛)》2009,(3)
比较不同程度感染牛病毒性腹泻病毒的六月龄牛群临床腹泻病和呼吸道疾病的累计死亡率。对101个具备不同感染条件农场犊牛的健康指标进行测试比较。不同程度感染牛病毒性腹泻病毒的犊牛群的累计死亡率和呼吸道疾病有很大关系。 相似文献
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<正>1流行特点牛病毒性呼吸道疾病会导致牛出现严重的呼吸道症状，发生混合感染，死亡率增加。常见的病毒性呼吸道疾病有：牛传染性鼻气管炎，致病病毒为牛传染性鼻气管炎病毒（IBRV）；犊牛病毒性肺炎，致病病毒为牛呼吸道合胞体病毒（BRSV）；牛病毒性腹泻，致病病毒为牛病毒性腹泻病毒（BVDV）；牛副流感，致病病毒为牛副流感病毒（BPIV）。牛病毒性呼吸道疾病秋冬、冬春交替时期较为流行。病毒能通过口腔、鼻腔、眼部分泌物， 相似文献
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为了确定甘肃省张掖市某奶牛场犊牛严重腹泻的病因,试验无菌采集发病犊牛血液、鼻黏液及粪便样品,通过病毒特异性引物的PCR检测、细菌分离鉴定及16S r DNA PCR检测及测序分析等方法对发病犊牛的病料进行检测,并对分离菌进行药敏试验。结果表明:全血及粪便等样品中牛病毒性腹泻病毒(BVDV)和牛传染性鼻气管炎病毒(IBRV)呈阳性;从粪便中分离出大肠杆菌,且分离株对氨苄西林、头孢氨苄、环丙沙星高敏,对其他抗菌药物分别呈现不同程度的耐药。说明该奶牛场犊牛腹泻是由BVDV、IBRV和大肠杆菌混合感染引起的,应结合药敏试验结果用药,同时配合中药辅助治疗,以提高临床治疗效果。 相似文献
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牛病毒性腹泻病毒RT-PCR检测方法的建立及应用 总被引：1，自引：1，他引：0
根据GenBank中登录的牛病毒性腹泻病毒(BVDV)基因序列,设计合成了1对特异性引物,建立了检测BVDV的RT-PCR方法。通过对该方法的特异性、敏感性和重复性进行试验,结果显示,该方法可从BVDV标准毒株Oregon C24V中扩增出471 bp的特异性片段,而对猪瘟病毒、牛传染性鼻气管炎病毒、牛呼吸道合胞体病毒、牛副流感病毒、MDBK正常细胞的扩增结果均为阴性。经对标准毒株的细胞毒进行检测,其敏感度达10-1 TCID50/mL。应用该方法对临床腹泻病牛各脏器样品进行检测,结果比病毒分离方法更为敏感,操作简便。表明建立的RT-PCR方法具有特异、灵敏、高效、快速的特点,可用于BVDV的临床检测及流行病学监测。 相似文献
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[目的]为了解凯里市A奶牛场牛呼吸道疫病的流行情况。[方法]本次采用酶联免疫吸附试验（ELISA）对142 份奶牛血清样品进行牛呼吸道合胞体病毒（BRSV）、牛病毒性腹泻（BVDV）、牛传染性鼻气管炎（IBRV）、牛支原体感染抗体检测。[结果]凯里市A奶牛场4 种呼吸道疾病病原普遍存在,BRSV阳性率为41.5%（59/142）;BVDV阳性率为40.1%（57/142）;IBRV阳性率为45.0%（64/142）;支原体阳性率为33.8%（48/142）。犊牛4 种呼吸疾病单一感染率为15.1%~45.5%;BVDV感染最严重,为45.5%;成年牛单一感染率为33.8%~45.1%。此外,奶牛场存在IBRV、BRSV、BVDV、支原体多种病原体混合感染,二重感染率为18.3％~30.2％;BVDV+IBRV感染率最高,为30.2%;三重感染以BVDV+支原体+IBRV感染率最高,为19.7%;四重感染率为9.9%。[结论]凯里市A奶牛场存在4 种疫病,有不同程度的病原体感染,应加强对上述病原体的流行控制,提高奶牛场管理水平,减少奶牛场疫病发生和经济损失。 相似文献
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为建立检测牛传染性鼻气管炎病毒(IBRV)血清抗体的阻断ELISA方法,本研究以经蔗糖密度梯度离心法纯化的IBRV作为免疫原制备1株单克隆抗体(MAb),命名为cp-1-1。经间接ELISA、IFA和western blot鉴定,该MAb与IBRV呈阳性反应,与牛病毒性腹泻病毒(BVDV)及牛副流感病毒3型(BPIV3)呈阴性反应,具有较强的特异性。质谱分析结果显示MAb cp-1-1识别的表位位于IBRV VP8蛋白。以纯化的IBRV作为包被抗原、MAb cp-1-1作为检测抗体,建立检测IBRV血清抗体的阻断ELISA方法。该检测方法的抗原包被量为0.89μg/孔,样品稀释度为12,检测抗体MAb量为1.3μg/孔,二抗稀释度为15000。利用50份IBRV抗体呈弱阳性的牛血清(中和抗体效价为14~116)作为标准参考血清,确定该检测方法的阻断率Cut Off值为52.06%,即阻断率高于52.06%时判为阳性,低于52.06%时判为阴性。阻断ELISA方法特异性试验显示仅IBRV阳性血清检测为阳性,而BVDV、BPIV3、牛腺病毒3型(BADV-3)和O型口蹄疫病毒(O-FMDV)阳性牛血清均检测为阴性,表明该方法具有较强的特异性;该方法可检测的最低中和抗体效价为14,与病毒中和试验的敏感性一致,表明该方法具有较高的敏感性;重复性试验显示该方法批内、批间变异系数均小于10%,显示较好的重复性。对130份现地牛血清检测结果显示,该方法与病毒中和试验的符合率为98.46%。用该方法对某牛场接种IBRV灭活疫苗的牛血清进行检测,抗体阳性率为99.51%(205/206)。另外,采用该方法对我国8个省(市、自治区)的801份牛血清进行检测,IBRV的抗体阳性率为41.6%(333/801)。本研究建立的阻断ELISA方法可以用于IBRV疫苗免疫监测和血清流行病学调查,为我国IBR的防控提供技术支持。 相似文献
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LOU Zhong-zi LIU Cong-nuan JIA Wan-zhong ZHOU Ji-zhang YAN Hong-bin CAO Xiao-an LI Li LI Zhao-cai FU Bao-quan 《中国畜牧兽医》2016,43(1):8-15
This study was aimed to establish a triple PCR method to rapidly identify Mycobacterium species, and evaluate its testing reliability.Three pairs of primer that were respectively specific to rv 3036c, rv 1970f and pncA genes of Mycobacterium were designed to establish a triple PCR for preliminary identification of Mycobacterium tuberculosis(M.tuberculosis), Mycobacterium bovis(M.bovis) and other Mycobacterium spp.PCR products were the expected sizes of 500(rv3036c), 125(rv1970f) and 249 bp(pncA), and contained two DNA bands(500 and 125 bp) with M.tuberculosis DNA template, two DNA bands(500 and 249 bp) with M.bovis DNA template.No band or non-specific band appeared with Mycobacterium spp.except M.tuberculosis and M.bovis DNA templates.The sensitivity of the triple PCR was calculated to 50 pg/μL template of genomic DNA.86 acid-fast bacteria were detected by the triple PCR, 16S rDNA and ITS gene sequencing, growth test and biochemical test, and the results were consistent between triple PCR and 16S rDNA and ITS gene sequencing.The detecting accuracy of triple PCR was 100%, and higher than growth test and biochemical test. 相似文献
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本研究旨在建立一种快速鉴定分枝杆菌的三重PCR方法,并比较分析其在临床检测中的可靠性。根据已发表的结核分枝杆菌、牛分枝杆菌和非洲分枝杆菌rv 3036c基因,结核分枝杆菌rv 1970f基因(RD7)和牛分枝杆菌pncA基因的序列,改造并设计合成了3对特异性扩增引物,建立了一种能对分枝杆菌样品进行初步鉴定的三重PCR方法。结果显示该方法可针对rv 3036c、rv 1970f和pncA基因分别扩增出大小为500、125和249 bp的目的片段,能特异性检测出结核分枝杆菌(500和125 bp两条带)和牛分枝杆菌(500和249 bp两条带),并可将结核分枝杆菌、牛分枝杆菌与其他分枝杆菌加以区分。本方法的检测灵敏度为50 pg/μL模板基因组DNA。对86株抗酸染色阳性菌进行三重PCR鉴定,鉴定结果与细菌16S rDNA和ITS序列测定结果一致,检测准确度为100%,优于生长特征和生化试验鉴定。 相似文献
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【目的】确定引起新疆石河子地区集约化牛场常发性肺炎的主要病原同时进行病原的体外药物敏感性分析。【方法】采集有典型咳嗽、流涕症状的牛鼻拭子10份和病死牛肺脏组织1份,用牛支原体特异性引物进行PCR检测,将检测为阳性的样本进行病原培养纯化,对纯化后的分离株菌落进行形态学观察、Dienes染色、生化试验及16S rRNA测序和进化分析,通过测定颜色变化单位(CCU)测定分离株生长曲线,并对分离株进行药物敏感性试验。【结果】PCR结果显示,10份鼻拭子中检测出7份牛支原体阳性样本,1份病死牛肺脏组织也检测为阳性;在涂有肺脏组织研磨液培养液的PPLO固体培养基上长出针尖状的菌落,纯化后分离株菌落形态为典型的煎蛋状;Dienes染色可见明显的深蓝色中心脐;生化试验结果显示,分离株不水解明胶、精氨酸、七叶苷,不发酵乳糖、葡萄糖和甘露醇,不分解尿素,可还原氯化三苯基四氮唑;16S rRNA测序结果显示,分离株与牛支原体国际标准株PG45相似性为99.7%,与国内牛支原体地方流行株XBY01、Ningxia-1、NM2012、Tibet-10的相似性最高,均为99.9%;生长曲线测定结果显示,分离株在培... 相似文献
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Comparative serological response in calves to eight commercial vaccines against infectious bovine rhinotracheitis, parainfluenza-3, bovine respiratory syncytial, and bovine viral diarrhea viruses 总被引：3，自引：2，他引：1 
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下载免费PDF全文 Van Donkersgoed J van den Hurk JV McCartney D Harland RJ 《The Canadian veterinary journal. La revue veterinaire canadienne》1991,32(12):727-733
A field trial was conducted to compare the serological responses in calves to eight commercial vaccines against infectious bovine rhinotracheitis virus (IBRV), parainfluenza-3 virus (PI3V), bovine respiratory syncytial virus (BRSV), and/or bovine viral diarrhea virus (BVDV). Calves given IBRV, P13V, BRSV, and BVDV vaccines had significantly higher antibodies to these viruses than unvaccinated controls; however, serological responses to killed BVDV vaccines were low. Calves with preexisting antibodies to IBRV, PI3V, BRSV, and the Singer strain of BVDV had lower seroconversion rates following vaccination than calves that were seronegative initially.
Serological responses in calves to IBRV, PI3V, BRSV, and BVDV differed among various commercial vaccines. Antibody titers to IBRV were higher in calves vaccinated with modified-live IBRV vaccines than in those vaccinated with killed IBRV vaccines. Following double vaccination with modified-live IBRV and PI3V vaccines, seroconversion rates and antibody titers to IBRV and PI3V were higher in calves vaccinated intramuscularly than in those vaccinated intranasally. Calves given Cattlemaster 4 had significantly higher titers to BRSV and PI3V, and lower titers to BVDV, than calves given Cattlemaster 3, suggesting that the addition of BRSV to Cattlemaster 4 caused some interaction among antigens.
相似文献18.
Restriction endonuclease analysis (REA) with three enzymes SmaI, PstI, BamHI- was used to identify 13 different genomic groups among 37 Mycoplasma bovis strains. One genomic group was comprised of 14 strains. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) patterns for one strain chosen from each genomic group and an international reference strain PG45 were all similar. Antigenic variability in M. bovis species was investigated by immunoblotting, using serum from a calf that had been naturally infected with M. bovis and three M. bovis-specific monoclonal antibodies — mAbs N2, I2 and 5D7. Twenty M. Bovis field strains were tested, comprising one from each genomic group, six from the same genomic group and the reference strain. Antigenic profiles obtained with calf serum differed markedly one from the other, the heterogeneity being equally great among the strains belonging to the same genomic group as those coming from different groups. A stable antigen common to 164 out of 168 strains was detected by mAb N2, whilst with mAbs I2 and 5D7, two different membrane antigenic systems were demonstrated that were strikingly variable. These variations in expression occurred not only from one strain to another, but also within the same lineage of clones from a single cell. 相似文献
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José C. Segura-Correa José L. Solorio-Rivera Laura G. Sánchez-Gil 《Tropical animal health and production》2010,42(2):233-238
Bovine viral diarrhoea virus (BVDV) and infectious bovine rhinotracheitis virus (IBRV) are important viral diseases around the world. The objective of this study was to estimate the incidence of seroconversion to BVDV and IBRV and to identify associated risk factors in dairy herds of Michoacan, Mexico. The longitudinal study included 62 herds and ran from December 2001 to November 2002. The total number of animals enrolled and completing the study were 392 and 342 animals for BVDV and 925 and 899 animals for IBRV. Animals were tested monthly for 12 months, for the presence of antibodies. Risk factors were: herd size (2–9, 10–25 and 26–55 animals), herd serostatus (seropositive or seronegative, only for IBRV), age group of the animal (6 to 12, 13 to 24, 25 to 48 and > 48 months) and animal origin (born in farm, purchased). The cumulative incidences for BVDV and IBRV were 16.4% and 3.4%, respectively; whereas, the incidence density rates for BVDV and IBRV were 15.9 and 2.9 per 1000 animal-months at risk, respectively. Seroconversion curves were statistically different for age group for BVDV and IBRV and for herd status for IBR. The relatively high incidence of seroconversion for BVDV suggests that a successful control programme should be oriented towards the identification and elimination of the PI animals and towards avoiding the introduction of PI cattle to the farm. The scenario of IBRV is favourable to implement a programme directed to reduce the number of new seropositive herds. 相似文献
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牛病毒性腹泻病毒一步法RT-PCR检测方法的建立与应用 总被引：2，自引：1，他引：1
为建立一种快速检测牛病毒性腹泻病毒病原的方法,本研究根据GenBank上登录的牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)基因组序列,设计1对引物,建立了检测BVDV的一步法RT-PCR方法。该方法对牛传染性鼻气管炎病毒、猪瘟病毒、牛副流感病毒3型的扩增结果均为阴性,检测的敏感性达1 ng RNA。该一步法RT-PCR方法具有良好的特异性、敏感性、重复性,可以准确快速检测出极低含量的BVDV,将为BVDV的病原检测及分子流行病学调查等提供一种快速、灵敏、特异、准确的分子生物学检测方法。 相似文献