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1.
Virucidal disinfectants and feline viruses 总被引：2，自引：0，他引：2
F W Scott 《American journal of veterinary research》1980,41(3):410-414
Thirty-five commonly used commercial disinfectants (disinfectants, antiseptics, sanitizers, and detergents) were evaluated for their virucidal activity against three feline viruses; feline viral rhinotracheitis virus (a herpesvirus), feline calicivirus, and feline panleukopenia virus (a parvovirus). Disinfectants were diluted as recommended by the manufacturer and were reacted with virus for 10 minutes at room temperature. Viruses were separated from disinfectants by gel filtration in special centrifuge tubes, and were assayed for infectivity in feline cell cultures. All 22 products tested were virucidal for feline viral rhinotracheitis virus, 11 of 35 were virucidal for feline calicivirus, but only 3 of 27 tested were effective against feline panleukopenia virus. A 0.175% sodium hypochlorite solution was the most effective and practical broad-spectrum virucidal product used alone or in combination with other disinfectants/detergents. 相似文献
2.
Oocysts of Cryptosporidium parvum are resistant to environmental conditions and many disinfectants. A combination of cell culture and quantitative real time PCR (cc–qPCR) is established for evaluation of anticoccidial disinfectants against C. parvum. C. parvum oocysts were treated with disinfectants, washed and oocysts were incubated with HCT-8 cell monolayers in the presence of excystation medium for 3 h. Subsequently, unbound parasites were removed by washing with growing medium and the infected monolayers were further maintained in fresh growing medium for 48 h. Genomic DNA was extracted from each sample and qPCR performed targeting a specific sequence of the 70 kDa heat shock protein gene in order to quantify development. Treatment of oocysts with cresolic disinfectants demonstrated dose dependent reduction of viability of oocysts. More than 98% inactivations were recorded with at least 2% concentration of cresolic disinfectants after 2 h of treatment. Bleach (sodium hypochlorite) at 6% solution induced 92.7% inactivation of C. parvum oocysts after 2 h. Thermally treated oocysts (56 and 70 °C for 20 min) demonstrated complete inactivation, whereas at 38 °C no inactivation was observed. Application of Neopredisan® 135-1 and Aldecoc® TGE (4% for 2 h) as recommended according to the current guidelines stipulated by DVG (German Veterinary Society) consistently inactivated more than 99.5% of oocysts. The suggested cc–qPCR method appeared to be suited for standardized testing of inactivation measures, particularly for evaluation of chemical disinfectants and thus cc–qPCR is proposed as an alternative to the established chicken infectivity model for Eimeria tenella for testing anticoccidial disinfectants. A minimum inactivation of 99.5% in cc–qPCR model is claimed as a suitable threshold for certification of chemical products for disinfection of coccidia oocysts. 相似文献
3.
SUMMARY Effects of storage at room temperature (23–25°C) and refrigeration (4–5°C) on various biochemical constituents of camel serum were investigated. Albumin, globulin, calcium, phosphorus, cholesterol, aspartate aminotransferase (AST), alkaline phosphatase (AP) and gamma glutamyltransferase (GGT) did not change over 9 days when stored at 4–5°C. At 4–5°C, creatinine, iron and glucose in camel sera remained stable for 6 days; total protein for 7 days; and blood urea nitrogen for 8 days. Decreased activities in creatine kinase (CK), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) were apparent after 1, 6 and 7 days, respectively. At room temperature, total protein, albumin, globulin, calcium and phosphorus were stable throughout the 9 days. Changes in glucose and iron occurred after 3 days. Stability at room temperature for LDH was 1 day; AST, 3 days; GGT and ALT, 6 days; and AP, 8 days. CK activity had already declined by 4 hours and by 9 days, only 34% activity remained. 相似文献
4.
Concentrated cell culture fluids of African horse sickness virus were shown to agglutinate erythrocytes from cattle, horses, sheep, goats, guinea pigs, rabbits, and poultry at 4°C, room temperature, and 37°C. The titres obtained were dependent on pH and NaCl molarity of the diluent, optimal titres being obtained at pH 7.5 and 0.6 M NaCl. The HA inhibiting antibodies to two AHS viruses were proven to be type specific. 相似文献
5.
I–2 is an avirulent strain of Newcastle disease virus. During establishment of the I-2 strain master vaccine seed, a series of selection procedures was carried out at 56°C in order to enhance heat resistance. This master seed is used to produce a working seed, which is then employed to produce
the vaccine. These two passages are done without further heat selection; however, it is not known how rapidly and to what
extent thermostable variants would be lost during further passage. The study was therefore conducted to determine the effect
of passage on thermostability of strain I-2. The virus was serially passaged and at various passage levels samples were subjected
to heat treatment at 56°C for 120 min. The inactivation rates for infectivity and haemagglutinin (HA) titres were assayed by use of chicken embryonated
eggs and HA test, respectively. Thermostability of HA and infectivity of I-2 virus were reduced after 10 and 5 passages, respectively,
without heat selection at 56°C. These results suggest that 5 more passages could be carried out between the working seed and vaccine levels without excessive
loss of thermostability. This would result in increased vaccine production from a single batch of a working seed. 相似文献
6.
Viruses may remain infectious outside the host cell for considerable time and represent a source of accidental infection if not properly inactivated. In this study, the survival of vesicular stomatitis virus (VSV) in suspension and dried on surfaces was analyzed. In addition, the sensitivity of VSV to disinfectants and physicochemical changes was investigated. VSV showed a notable stability in suspension at 4 °C with virus titers remaining high over several weeks. The presence of serum proteins had a stabilizing effect on virus infectivity, whereas elevated temperatures reduced survival times. VSV dried on polystyrene, glass or stainless steel surfaces remained infectious for at least 6 days at ambient temperature. VSV showed a remarkable resistance to extreme pH in particular in the alkaline range, but could be rapidly inactivated by heating at 55 °C or higher. The virus was highly sensitive to inactivation by commonly used disinfectants such as aldehydes, alcohols, and detergents. The high stability of VSV on surfaces and in suspension may facilitate dissemination of the virus in livestock by contaminated feeding and water troughs, hands, and milking equipment. This knowledge on the sensitivity of VSV to disinfectants will help to set up appropriate hygiene measures. 相似文献
7.
The persistence of foot-and-mouth disease virus on wool 总被引：1，自引：0，他引：1
SUMMARY Five Suffolk sheep, held in a high-security isolation room, were exposed for 2 hours to the aerosol of 3 mature pigs that had been infected with foot-and-mouth disease virus (FMDV), strain O1-BFS. The fleeces of 3 of the sheep were contaminated with FMDV at 2 days post exposure (dpe), while at 5 dpe the fleeces of all 5 sheep were more extensively, and more heavily, contaminated. The persistence of FMDV on contaminated wool was examined in vitro using multiple 0.5 g samples of Merino wool that were each contaminated with one of 3 strains of FMDV in tissue-culture medium: O1-BFS, O-Morocco (O-MOR 9/91) or an Asia 1 strain (TAI 1/90). Wool samples were held at either 4°C, 18°C or 37°C, and decay curves were established for each virus at each temperature. These curves predicted that O1-BFS, O-MOR 9/91 and TAI 1/90 would fall below detect-able levels at 72, 70 and 48 days post contamination (pc), respectively, for wool stored at 4°C; at 11, 12 and 12 days pc, respectively, for wool stored at 18°C; and at 57, 68 and 33 hours pc, respectively, for wool stored at 37°C. For wool contaminated with O1-BFS-infected sheep faeces, urine or blood, or with O1-BFS-infected cattle saliva, decay curves predicted virus to persist for 5 to 11 days pc at 18°C. We demonstrated that the simulated scouring of FMDV-contaminated wool at 60° to 70°C would usually reduce virus to below detectable levels. The detergent component of the scouring process had little, if any, antiviral activity, and scouring at 20°C or 50°C had limited impact on FMDV titres . We recommend that either (1) simple storage of FMDV-contaminated wool for 4 weeks at temperatures of 18°C or higher, or (2) scouring of contaminated wool at 60° to 70°C would be sufficient to remove the threat of FMDV-contaminated wool being infectious to other animals . 相似文献
8.
D T Shen T B Crawford J R Gorham T C McGuire 《American journal of veterinary research》1977,38(8):1217-1219
Twelve chemicals and commercial disinfectants were tested for inactivation of equine infectious anemia virus. In the presence of 10% bovine serum, all chemicals inactivated 4 log10 (based on 0.1 ml) of the virus within 5 minutes at 23 C. A reduction of at least 4 log10 was observed when the virus was exposed for 1 minute to substituted phenolic disinfectants (3 commercial preparations and sodium orthophenylphenate), halogen derivatives (iodophor and sodium hypochlorite), chlorhexidine, and 70% ethanol. Sodium hydroxide (5%), 2% formalin, and 2% glutaraldehyde were slower to inactivate the virus, but achieved 4 log10 reduction in titer by 5 minutes' contact time. The susceptibility of the equine infectious anemia virus to chemical disinfectants is similar to that of other enveloped viruses. 相似文献
9.
The Newcastle disease virus (NDV) occurring in Australia is apathogenic for chickens following natural infections. Some properties of the avirulent Australian V4 strain of NDV and of 12 new isolates of NDV were compared.The viruses grew to high titres following infection of chick embryos by the allantoic cavity and allantoic fluid had infectivity titres of from . With only two isolates did sufficient mortalities occur to allow calculation of mean death times and these were in excess of 140 h. Five of nine isolates failed to kill 100% of embryos when doses in excess of 107·9 EID50 were used. When strain V4 was inoculated into the yolk sac of 10-day-old embryos, the LD50 was similar to the ID50 obtained with allantoic cavity inoculation, and the mean death time was 103 h.The intracerebral pathogenicity index for strain V4 was 0.91 and 1.02 in two experiments. The index was not significantly reduced when the virus was taken through a further cycle of plaque purification or when the inoculum was heated at 56°C for 30 min. Chickens with maternally derived antibody to NDV were not susceptible to intracerebral inoculation with strain V4. Chickens dying after intracerebral inoculation with strain V4 had haemorrhagic and necrotic liver lesions. The intracerbral pathogenicity indices for four other isolates varied from 0 to 0.22.The infectivity of V4 and three other isolates was relatively stable at 56°C and that of another eight isolates was labile. Haemagglutinins of all viruses studied were stable at 56°C for longer than 60 min. None of four isolates tested lost haemagglutinin activity on treatment with ether.Haemagglutination-elution patterns were variable but four isolates did not elute from chicken erythrocytes after 24 h at 4°C and strain V4 and isolate PM12 did not elute after 96 h at 4°C. Six viruses, including V4, agglutinated erythrocytes from all of six test horses. The haemagglutinin activity of the remaining viruses varied between horses.Four viruses including V4 haemolysed chicken erythrocytes. Gradient centrifugation allowed the separation of an infectious and a noniffectious haemagglutinin. Haemolytic activity was associated with the infectious haemagglutinin. 相似文献
10.
Thomovsky EJ Backus RC Mann FA Richmond CK Wagner-Mann CC 《American journal of veterinary research》2008,69(5):652-658
OBJECTIVE: To determine whether lipid particle coalescence develops in veterinary parenteral nutrition (PN) admixture preparations that are kept at room temperature (23 degrees C) for > 48 hours and whether that coalescence is prevented by admixture filtration, refrigeration, or agitation. SAMPLE POPULATION: 15 bags of veterinary PN solutions. PROCEDURES: Bags of a PN admixture preparation containing a lipid emulsion were suspended and maintained under different experimental conditions (3 bags/group) for 96 hours while admixtures were dispensed to simulate IV fluid administration (rate, 16 mL/h). Bags were kept static at 4 degrees C (refrigeration); kept at 23 degrees C (room temperature) and continuously agitated; kept at room temperature and agitated for 5 minutes every 4 hours; kept static at room temperature and filtered during delivery; or kept static at room temperature (control conditions). Admixture samples were collected at 0, 24, 48, 72, and 96 hours and examined via transmission electron microscopy to determine lipid particle diameters. At 96 hours, 2 samples were collected at a location distal to the filter from each bag in that group for bacterial culture. RESULTS: Distribution of lipid particle size in the control preparations and experimentally treated preparations did not differ significantly. A visible oil layer developed in continuously agitated preparations by 72 hours. Bacterial cultures of filtered samples yielded no growth. CONCLUSIONS AND CLINICAL RELEVANCE: Data indicated that the veterinary PN admixtures kept static at 23 degrees C are suitable for use for at least 48 hours. Manipulations of PN admixtures appear unnecessary to prolong lipid particle stability, and continuous agitation may hasten lipid breakdown. 相似文献
11.
The role of ketamine (K) in pain management is controversial. It is reported to provide visceral analgesia in cats. This study aimed to assess its somatic actions using a thermal threshold (TT) model. Six cats (four spayed females, two castrated males, 4.3–7.2 kg) participated in the study. The day before each study, the thorax of each of the cats was shaved and a cephalic catheter was placed. TT was measured using a device specifically developed for cats. A heater element and temperature sensor housed in a small probe were held against the thorax of the cats with an elastic band and pressure bladder to assure consistent contact. The skin temperature was recorded before each test, then the heater was activated. When the cat responded by flinching, turning, or jumping, the stimulus was terminated and the threshold temperature was recorded. Treatments were 2 mg kg?1 of K (10 mg mL?1), or 0.2 mL kg?1 of saline (S) IV, given in a randomized cross‐over design with at least 1 week between treatments. The investigator was blinded to the treatment. TT was measured thrice before treatment (baseline threshold) at 15 minutes, then every 30 minutes for 8 hours and once at 24 hours after injection. Data were analyzed using a four‐factor anova . Cats were sedated for 45 minutes following K treatment. There was no difference in baseline TT between treatments (K = 41.9 ± 1.7 °C, S = 41.0 ± 1.45 °C), and no change in TT at any time in the S group. TT increased significantly at 15 and 30 minutes after K, then decreased below baseline values between 210 and 390 minutes, with a nadir of 38.8 ± ± 1.05 °C at 390 minutes. During this time period, cats exhibited normal activity, but responses to thermal stimuli were exaggerated. This study suggested that K caused a delayed onset hyperalgesia in cats. 相似文献
12.
Factors affecting the survival of Streptococcus suis type 2 总被引：6，自引：0，他引：6
The survival of Streptococcus suis type 2 was assessed in experimentally inoculated faeces and dust stored at 0, 9 and 22 to 25 degrees C. The organism survived in faeces for 104 days at 0 degrees C, up to 10 days at 9 degrees C and up to eight days at 22 to 25 degrees C. It survived in dust for up to 54 days at 0 degrees C and up to 25 days at 9 degrees C but could not be isolated from dust stored at room temperature for 24 hours. The organism survived at 4 degrees C in nutrient medium for up to nine months but in distilled water for only one to two weeks. At 50 degrees C it survived in water or broth for up to two hours but at 60 degrees C it only survived for 10 minutes. The organism was rapidly inactivated by disinfectants and cleansers, commonly used on farms and in laboratories, at concentrations less than those recommended for use by the manufacturers. 相似文献
13.
Elia G Cavalli A Cirone F Lorusso E Camero M Buonavoglia D Tempesta M 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2005,52(7-8):320-322
The relationship between maternally derived antibody (MDA) levels and protection to canine parvovirus (CPV) infection in pups is reported. Twelve pups with a wide range of haemagglutination inhibiting (HI) titres of MDA to CPV were divided into four groups, with each group balanced for antibody titres. The dogs were inoculated with a field CPV-2b strain and clinical signs, virus shedding and antibody response were assessed. The CPV was not detected in the faeces of dogs with HI titres of 320 at any time. In dogs with HI titres up to 160, active CPV replication after challenge was demonstrated by real-time polymerase chain reaction. The successful infection of dogs with HI titres of 80 and 160 was confirmed by seroconversion, evaluated at day 14 post-infection. These findings demonstrated that CPV infection could also occur in the presence of MDA HI titres (> or =80) usually considered fully protective. 相似文献
14.
Annabelle Olsson David Phalen Christina Dart 《Veterinary anaesthesia and analgesia》2013,40(5):494-502
ObjectivesTo investigate the character of immobilization given by alfaxalone in juvenile crocodiles at optimal and at suboptimal temperatures.Study designProspective, randomized partial crossover study.AnimalsTwenty captive male estuarine (weight 0.6–2.5 kg) and five captive male freshwater crocodiles (weight 0.2–0.6 kg).MethodsCrocodiles were acclimatized for 24 hours at one of the following environmental temperatures; 32 °C, 27 °C, 22 °C or 17 °C, then received 3 mg kg?1 intravenous (IV) alfaxalone into the dorsal occipital venous sinus. Duration and quality of immobilization was assessed and heart rate (HR) measured. On a separate occasion each crocodile was immobilized at one other environmental temperature.ResultsAlfaxalone, 3 mg kg?1 IV, produced immobilization for 55 (range 15–100 minutes in estuarine, and 20 (range 20–25) minutes in freshwater crocodiles at 32 °C. There was no significant difference overall in immobilization times between temperatures, other than that, in estuarine crocodiles, duration was shorter at 32 °C than 22 °C. The character of immobilization was unpredictable, with animals recovering without warning, or having extended recoveries requiring assisted ventilation. Assisted ventilation was necessary mainly at the lower temperatures. Median HR in all temperature treatments decreased within 5 minutes post–injection, but the change in HR over the duration of immobilization was affected by the temperature, with a progressively smaller range of fall as temperature decreased. At 17 °C, two estuarine crocodiles appeared to re–immobilize after initial recovery, became severely bradycardiac and required ventilation and re–warming.Conclusions and clinical relevanceAlfaxalone IV in small captive estuarine and freshwater crocodiles provides adequate induction of immobilization at various temperatures. However, the unpredictable results following induction mean it is unsuitable for field use and should be restricted to environments where intubation and ventilation are available, where animals can be warmed to optimal temperature, and where access to immersion in water can be restricted for 24 hours. 相似文献
15.
Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine 总被引：1，自引：1，他引：0
P. N. Wambura 《Tropical animal health and production》2009,41(5):797-802
The formulation and evaluation of trehalose nano-organogels for storage and oral delivery of Newcastle disease (ND) strain
I-2 vaccine to chickens were carried out in this study. Trehalose sugar was blended with vegetable oil to form nano-organogels
where trehalose also acted as a stabilizer against thermal inactivation of I-2 ND virus. Results from infectivity titration
assay indicated that the titre of 107.5 EID50/0.1 mL was maintained after 12 weeks of storage of nano-organogel I-2 vaccine at ambient room temperature. Serology results
showed that 33% chickens which were vaccinated with nano-organogel I-2 vaccine after 14 days had HI antibody titres of ≥ 3.0 log2 with GMT of 2.3. Moreover, results showed 100% of chickens vaccinated with nano-organogel I-2 vaccine had the mean antibody
titres of 3.4 and 3.7 log2 at 21 and 28 days after vaccination, respectively. All vaccinated chickens (100%) survived the challenge of virulent ND virus
whereas all unvaccinated chickens succumbed to challenge and died of signs consistent with ND. The findings from this study
showed that the nano-organogel I-2 vaccine was stable at room temperature, safe and produced protective antibody response
in vaccinated chickens. Moreover the nano-organogel I-2 vaccine was used for oral administration and hence is suitable for
mass vaccination. However, optimization of the formulation of trehalose nano-organogel vaccine is required in order to achieve
its application potentials. 相似文献
16.
Skim milk and whey prepared from milk secreted by cows that had been injected with bacteria were kept under various conditions, and the antigen‐binding and protein G‐binding abilities of immunoglobulin G (IgG) in the milk samples were investigated. More than 85% of the original antigen‐binding and protein G‐binding abilities remained when the skim milk was heated at 63°C for 60 min or kept at a pH range of 4–10 for 24 h at 37°C. Both of the high abilities were maintained on pepsin treatment above pH 4 and intestinal proteinase treatments. Little antigen‐binding and protein G‐binding abilities decreased upon storage of the powdered skim milk for 12 months at room temperature. In contrast, at least half, and more than 70% of their respective abilities, were lost upon incubation at pH 2 in the absence and presence of pepsin, respectively, for 1 h at 37°C. These results suggest that most of the biological activities of the Fab‐region and Fc‐region of bovine milk IgG can be maintained during processing or storage, and both of the inactivation degrees mainly depend on the stomach condition after oral ingestion of the IgG preparation. 相似文献
17.
Pratelli A 《Zoonoses and public health》2007,54(9-10):383-386
Canine coronavirus (CCoV) is responsible for enteric disease in pups. Infected dogs generally have a rapid recovery, so the virus is highly contagious and the spread of infection is difficult to control. Chemical disinfectants have been widely used in human disease-control programmes to prevent viral infectious diseases from spreading, but to date, there are no studies in the literature on the sensitivity of CCoV to chemical biocides. The present study investigated the sensitivity of CCoV to disinfectants currently used for prophylaxis in kennel and dog breeding locations. The effects of three agents: alkyl-dimethyl-benzyl-ammonium chloride, benzalkonium chloride and didecyl-dimethyl-ammonium chloride, on the infectivity titre of CCoV in A72 cell lines, were studied at different concentrations. Although they may regard a small number of agents, the findings showed that the sensitivity of CCoV to disinfectants varies and the differences are dose correlated. In general, virus inactivation implies a permanent loss of infectivity which can be evaluated in suspensions and hand disinfection tests. 相似文献
18.
Yamamoto M Horiuchi M Ishiguro N Shinagawa M Matsuo T Kaneko K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):983-990
Agents of transmissible spongiform encephalopathy (prion) are known to be extremely resistant to physicochemical inactivation procedures such as heat, radiation, chemical disinfectants such as detergents, alcohols, glutaraldehyde, formalin, and so on. Because of its remarkable resistance, it is difficult to inactivate prion. Chemical inactivation seems to be a practical method because it is applicable to large or fixed surfaces and complicated equipment. Here, three epoxides: beta-propiolactone, propylene oxide, and glycidol (GLD) were examined of their inactivation ability against scrapie-mouse prion protein (PrP(Sc)) under various conditions of chemical concentration, incubation time, and temperature. Among these chemicals, GLD worked most effectively and degraded PrP into small fragments. As a result of the bioassay, treatment with 3% GLD for 5 hr and 5% GLD for 2, 5 hr or 12 hr at room temperature prolonged the mean incubation time by 44, 30, 110 and 73 days, respectively. From dose-incubation time standard curve, the decrease in infectivity titers were estimated as 10(3) or more. Therefore, degradation of PrP(Sc) by GLD decreased the scrapie infectivity. It is also suggested that pH and salt concentrations influence the effect of GLD. Although further study is necessary to determine the optimal condition, GLD may be a potential prion disinfectant. 相似文献
19.
Ameri M Schnaars HA Sibley JR Honor DJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(2):188-193
Background: The time from sampling to analysis can be delayed when blood samples are shipped to distant reference laboratories or when analysis cannot be readily performed. Objective: The objective of this study was to evaluate the stability of hematologic analytes in blood samples from monkeys, rabbits, rats, and mice when samples were stored for up to 72 hours at 4°C. Methods: Blood samples from 30 monkeys, 15 rabbits, 20 rats, and 30 mice were collected into EDTA‐containing tubes and were initially analyzed within 1 hour of collection using the ADVIA 120 analyzer. The samples were then stored at 4°C and reanalyzed at 24, 48, and 72 hours after collection. Results: Significant (P<.0003) changes in hematologic analytes and calculations included increased HCT and MCV and decreased MCHC and cell hemoglobin concentration mean (CHCM) at 72 hours and increased MPV at 24 hours in monkeys; increased MCV at 72 hours and MPV at 48 hours and decreased monocyte count at 24 hours in rabbits; increased MCV and decreased MCHC, CHCM, and monocyte count at 24 hours in rats; increased MCV, red cell distribution width, and MPV and decreased MCHC, CHCM, and monocyte count at 24 hours in mice. Conclusions: Although most of the changes in the hematologic analytes in blood from monkeys, rabbits, rats, and mice when samples were stored at 4°C were analytically acceptable and clinically negligible, the best practice in measuring hematologic analytes in these animals is timely processing of blood samples, preferably within 1 hour after collection. 相似文献
20.
The effect of a pre‐anesthetic infusion of amino acids on body temperature,venous blood pH,glucose, creatinine,and lactate of healthy dogs during anesthesia 
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下载免费PDF全文 Stuart C Clark‐Price Olivier Dossin Thandeka R Ngwenyama Mauria A O'Brien Maureen McMichael David J Schaeffer 《Veterinary anaesthesia and analgesia》2015,42(3):299-303
ObjectiveTo evaluate the effect of preanesthetic, intravenous (IV) amino acids on body temperature of anesthetized healthy dogs.Study designRandomized, experimental, crossover study.AnimalsEight mixed-breed dogs approximately 2 years of age weighing 20.7 ± 2.1 kg.MethodsDogs received 10% amino acid solution (AA) or 0.9% saline (SA) IV at 5 mL kg−1 over 60 minutes. Body temperature (BT) was recorded at 5 minute intervals during infusions. Dogs were then anesthetized with sevoflurane for 90 minutes. BT was recorded at 5 minute intervals during anesthesia. Jugular blood samples were analyzed for pH, glucose, creatinine, and lactate concentrations at baseline, after infusion, after anesthesia and after 24 hours.ResultsBT at conclusion of infusion decreased -0.34 ± 0.42 °C in group AA and -0.40 ± 0.38 °C in group SA and was not different between groups (p = 0.072). BT decreased 2.72 ± 0.37 °C in group AA and 2.88 ± 0.26 °C in group SA after anesthesia and was different between groups (p < 0.05). Creatinine in group AA was increased immediately after infusion (p < 0.0001) and at 24 hours (p < 0.0001). There were no differences between groups for other parameters. Values for both groups were never outside the clinical reference ranges.Conclusions and clinical relevanceIn healthy dogs, preanesthetic IV infusion of amino acids attenuated heat loss compared to controls, however, the amount attenuated may not be clinically useful. Further studies are warranted to determine if nutrient-induced thermogenesis is beneficial to dogs undergoing anesthesia. 相似文献