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雏鹅新型病毒性肠炎病毒弱毒株的培育及其特性研究 总被引：1，自引：0，他引：1
应用在鸭胚成纤维细胞上连续传代的方法，获得了由雏鹅新型病毒性肠炎病毒强毒株（NGVEV－CN株）致弱的弱毒株（CN40株）。该弱毒株TCID50为10^-8.083，具有良好的安全性和稳定性，在易感1日龄雏鹅连传8代不返强。该弱毒具有良好的免疫原性，最小免疫剂量为10000TCID50，免疫后3d产生部分免疫力，5d产生坚强免疫力。口服免疫效果最好，对雏鹅免疫期在30d以上。该弱毒株能够干扰强毒在雏鹅体内繁殖，进入鹅体后能够进行一定程度繁殖并排泄出体外，使同居雏鹅感染并获得一定程度免疫力。临床使用后可极显著降低雏鹅的死亡率。 相似文献
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雏鹅新型病毒性肠炎病毒(NGVEV)是一种能引起30日龄以内雏鹅感染发病的病毒,其流行病学、临床症状、病理变化与小鹅瘟(GP)相似,因而,在实际生产中,常将其误诊为GP.种鹅在开产前注射1次GP疫苗,但其子代于3日龄仍出现发病和死亡情况,用GP高免血清治疗无效.后经病毒分离、人工感染雏鹅、电镜观察病毒粒子、免疫接种和治疗等方法,将该种现象确诊为是由腺病毒引起的一种尚未被认识的新型病毒性肠炎. 相似文献
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雏鹅新型病毒性肠炎(NGVE)是由一种腺病毒引起的雏鹅肠炎的新型传染病。本病一年四季均可发生,目前山东主要发生于30日龄以内从外地引进的雏鹅。该病发病率较高,一般为25%～75%,有时高达100%,传播速度快,严重危害养鹅业的健康发展。其典型病例,肠道有类似于小鹅瘟的“香肠样”病变,但种鹅开产前注射一次小鹅瘟疫苗或雏鹅孵出后注射高免血清后,其雏鹅于3日龄后仍然发病和死亡,发病后注射大剂量的高免小鹅瘟血清,同时附以各种抗生素、抗病毒药物治疗,均不见效。1病原及其特性本病初步被认为由腺病毒引起,病毒(NGVEV)呈球形或椭圆形,无囊膜… 相似文献
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雏鹅新型病毒性肠炎是由一种新型腺病毒引起的雏鹅传染病。可造成雏鹅大批死亡,具有传播快、发病率高、死亡率高的特点。目前在全国各地时有发生,给养鹅业造成了巨大损失。 相似文献
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<正>1.小鹅瘟雏鹅疫苗。适用于未经免疫种鹅的后代雏鹅或种鹅免疫后已达到7～8个抗体滴度以上的雏鹅作紧急预防接种。使用时按瓶签注明剂量,即按1:100倍稀释,给出壳后24小时以内的雏鹅皮下注射0.1毫升,接种后7天产生主动免疫力。 相似文献
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雏鹅新型病毒性肠炎于1997年首次被发现,是雏鹅的一种卡他性、出血性、纤维性渗出及坏死性肠炎,其临床症状和病理变化与小鹅瘟非常相似。通过对该病的病毒分离、鉴定、病原学特性的研究,证明了雏鹅新型病毒性肠是由雏鹅的一种新的腺病毒引起的、不同于小鹅瘟的新传染病。1流行特点 相似文献
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1小鹅瘟雏鹅疫苗
适用于未经免疫接种鹅后代的雏鹅,或种鹅免疫后已达到7～8个抗体滴度以上,可作雏鹅紧急预防接种。在使用时可按瓶签注明的剂量,可按1：100倍稀释,给出壳后24h以内的雏鹅,皮下注射0.1ml/只,7d后产生主动免疫力。疫苗可放置在-15℃以下冷冻保存,有效期18个月。 相似文献
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根据雏鹅新型病毒性肠炎病毒(NGVEV)保守基因序列,设计合成一对引物并且进行PCR检测,扩增出预期的223 bp条带。该方法能在雏鹅新型病毒性肠炎病毒BC07株中扩增到特异性片段,而鹅细小病毒、鸭瘟病毒、鹅副黏病毒的扩增结果均为阴性;敏感性试验表明该方法最低检出限量为2.5×10~(-1)EID_(50)。表明所建立的PCR方法具有敏感性高、特异性强的特点。可以用来检测雏鹅新型病毒性肠炎。 相似文献
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5～10周龄扬州鹅日粮中适宜蛋氨酸水平的研究 总被引：2，自引：0，他引：2
本文旨在研究5～10周龄扬州鹅日粮中适宜的蛋氨酸水平。选用28日龄的健康扬州鹅150只,随机分成5组,每组3个重复,每个重复10只,公母各半。试验采用单因子有重复设计,日粮蛋氨酸水平分别为0.23%、0.33%、0.43%、0.53%和0.63%。结果表明:日粮不同蛋氨酸水平对5～10周龄扬州鹅生长性能、屠宰性能和血清生化指标均存在显著影响。(1)0.43%蛋氨酸水平试验组鹅的生长速度最快,料重比显著低于0.23%组(P<0.05);(2)0.43%和0.53%蛋氨酸水平试验组鹅在屠宰率、半净膛率、全净膛率、胸肌率和腿肌率显著高于其他各组(P<0.05),且随着日粮中蛋氨酸水平的增加腹脂率呈下降趋势,0.53%和0.63%组鹅腹脂率显著低于0.23%组(P<0.05);(3)0.43%和0.53%蛋氨酸水平试验组鹅血清尿素氮(BUN)浓度显著低于0.23%组(P<0.05),0.53%蛋氨酸水平试验组鹅血清总蛋白(TP)浓度显著高于0.23%组(P<0.05)。综上所述,当日粮蛋白质水平为15.82%时,建议5～10周龄扬州鹅日粮中适宜蛋氨酸水平为0.43%～0.53%。 相似文献
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Shun Chen Anchun Cheng Mingshu Wang Dekang Zhu Qihui Luo Fei Liu Xiaoyue Chen 《Veterinary immunology and immunopathology》2009,130(3-4):226-235
To determine the distribution and localization of new type gosling viral enteritis virus (NGVEV) in paraformaldehyde-fixed paraffin-embedded tissues of experimentally infected goslings, for the first time, an immunohistochemical (IHC) staining method was reported. Anti-NGVEV polyclonal serum was obtained from the rabbits immunized with purified NGVEV antigen, which was extracted by caprylic-ammonium sulphate method and purified through High-Q columns anion exchange chromatography. Three-day-old NGVEV-free goslings were orally inoculated with NGVEV-CN strain suspension as infection group and phosphate buffered saline solution (PBS) as control group, respectively. The tissues were collected at sequential time points between 0.5 and 720 h post inoculation (PI), and prepared for IHC staining and ultra-structural observation. The positive immunoreactivity could be readily detected in the lymphoid and gastrointestinal organs of infected goslings as early as 48 h PI, in the liver, kidney, pancreas and myocardium from 72 h, and in the cerebrum and cerebellum from 96 h, while it was hardly detected in the respiratory organs at any time. The positive staining reaction could be detected in NGVEV-infected goslings until 600 h PI, and no positive staining cell could be observed in the controls. The highest levels of viral antigen were found in the bursa of Fabricius (BF), thymus, proventriculus, gizzard and intestine tract, moreover, the liver, kidney, spleen, myocardium and pancreas were intensively and widely stained. The target cells had a ubiquitous distribution, especially included the epithelial cells, endothelial cells, superficial and crypt mucosal cells, glandular cells, fibrocytes, macrophages and lymphocytes, which served as the principal sites for antigen localization. The ultra-structural observation by transmission electron microscope (TEM) further indicated that NGVEV particles could be widely detected in the lymphoid and digestive organs of infected goslings from 72 h PI onwards. This work may be useful not only for offering a possibility of routine diagnosis of NGVE, but also for better understanding of the pathogenesis of the disease. 相似文献
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1.?The purpose of this study was to investigate the effects of different sources of dietary non-starch polysaccharides (NSP) on growth performance, development of gastrointestinal tract, and activities of pancreatic enzymes in goslings from 0 to 21 d of age.
2.?A total of 100 one-day-old White Roman female goslings were selected and randomly divided into 5 dietary treatment groups. Each group had 4 replicate cages (100 × 60 × 58 cm) of 5 goslings. Goslings in each group were given one of 5 isoenergetic and isonitrogenous semi-purified diets. Experimental diets were designed to contain 90 g/kg of different sources of dietary NSP from maize (control), barley hull, rice bran, wheat bran, or pectin, respectively, and 97·4 g/kg NSP were supplied by soybean meal.
3.?The goslings receiving the pectin diet had significantly higher viscosity in intestinal digesta than the other treatment diets. However, the daily feed intake, daily weight gain, and feed conversion ratio of goslings in the pectin group were significantly lower than those given the other diets.
4.?The relative weights of proventriculus, gizzard, liver and pancreas, as well as the relative weights and lengths of various intestinal segments in the goslings that received the pectin diet, were significantly higher than those receiving the other diets. The specific activities (SA) of amylase, lipase, trypsin, and chymotrypsin in the pancreatic tissue of goslings in the pectin group were significantly lower than those in the other groups.
5.?Goslings given a soluble NSP source (pectin) diet tended to exhibit inhibited growth and decreased activity of pancreatic enzymes. However, compared with maize, the inclusion of insoluble NSP sources from barley hull, rice bran, and wheat bran did not negatively affect growth and also were not significantly different among the treatment diets. 相似文献
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王璐 《畜牧兽医科学(电子版)》2021,(5):167-168
鹅是草食水禽类动物,具有独特的生物学特征和生理特征。雏鹅是指21日龄内的小鹅,该阶段鹅绒毛稀少,身体较小,体温调节能力不足,消化机能不健全,对外界环境的适应能力和抗病能力普遍较弱,一旦饲养管理不当,没有进行妥善的疫苗免疫接种,易造成病原的传播流行,引发死亡。在雏鹅阶段,应充分掌握引发死亡的具体原因,并从养殖管理等诸多环节入手,为雏鹅营造健康舒适的生长环境,提高群体的身体抵抗能力,降低发病率和死亡率,确保养殖安全。该文主要结合一个鹅场的养殖案例,论述引起雏鹅死亡的原因,并提出相应的防治措施。 相似文献
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Six cases of fowl cholera in growing turkeys and 3 in adult breeder chickens of the broiler type as well as one case each of a Pasteurella (P.) multocida-associated disease in ducklings and goslings were described in consideration of own laboratory findings and available informations of the case history. Furthermore a report is given on a treatment strategy successfully used in turkeys with highly acute fowl cholera. All the P. multocida strains isolated culturally could be assigned to the subspecies multocida. In one case Bordetella avium, Salmonella (S.) arizonae and S. hadar were additionally cultured form part of turkeys submitted. P. multocida and Moraxella (Pasteurella) anatipestifer could be determined as the causative agents of the disease of ducklings and goslings. P. multocida strains from turkeys were identified serologically as serovars A:3.4 (3x), F:3.4 (2x) and A:3 (1x); those from the breeder chickens as A:3 (3x); and one each from ducklings and goslings as F:3.4 and -:3. (uncapsulated). No death occurred in turkeys with clinical signs of a highly acute fowl cholera if the treatment of the affected birds was started with an intravenous injection of sulfadimethoxine and continued with a combination of sulfachlorpyridazine (SCP) and trimethoprim (TMP) given in the drinking water for 5 days. However relapse occurred 2-3 days after withdrawal of the drug, although the therapy was clinically highly effective. The recurrence of the disease could be prevented reliably if the turkeys were vaccinated with an effective oil-based bacterin and subsequently treated with the SCP-TMP combination given in drinking water over a 12 day period. 相似文献
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Induction of strong protection by vaccination with partially attenuated serotype 1 Marek's disease viruses 总被引：3，自引：0，他引：3
Witter RL 《Avian diseases》2002,46(4):925-937
Studies were conducted to better understand the relationship among Marcek's disease (MD) vaccine strains between induction of protective immunity and the degree of attenuation (or virulence). To obtain viruses at different stages of attenuation, very virulent plus MD strains 584A and 648A and selected clones of these strains were serially passaged in chicken and duck cells. These viruses were considered fully attenuated after passage for 70-100 times in chicken embryo cell cultures until they no longer induced gross lesions in susceptible, maternal antibody-negative (ab-) chickens. Lower passages of the same strains were considered partially attenuated, provided their virulence was less than that of the parent strain. Four of five partially attenuated preparations derived from MD virus strains 584A and 648A or the previously attenuated Md11 strain induced 28%-62% higher levels of protection in maternal antibody-positive (ab+) chickens against virulent MD challenge than the fully attenuated counterpart viruses. The partially attenuated 584A/d2/3 strain replicated in chickens but was totally nonprotective. Data from two subsequent trials in ab+ chickens confirmed that protection induced by the partly attenuated (passage 80) preparations was 79% and 118% higher, respectively, than that induced by the fully attenuated (passage 100) preparations of strain 648A. However, in one trial with ab- chickens, no difference in protection between partially and fully attenuated virus was observed. Strong protection (up to 85%) against highly virulent challenge also was provided by preparations of 648A at passages 40-60, which were moderately oncogenic when used alone. Partially attenuated strains tended to replicate to higher titers in both ab+ and ab- chickens compared with fully attenuated vaccines. Also, ab+ and ab- chickens vaccinated with partially attenuated strains developed three- to nine fold more extensive microscopic lesions in peripheral nerves at 14 and 22 days after virulent challenge than chickens vaccinated with fully attenuated strains. When measured in ab+ chickens, loss of lesion induction by 648A was achieved 30 passages earlier (at passage 70) than when measured in ab- chickens. Thus, maternal antibodies appeared to abrogate the pathogenicity of some partially attenuated strains. These studies establish for MD the principle that at least some partially attenuated MD viruses may replicate better and induce stronger immunity against virulent challenge than fully attenuated preparations of the same strain, at least when tested in ab+ chickens. Moreover, depending on passage level, partially attenuated vaccine strains may be relatively innocuous for ab+ chickens, causing few or no lesions. 相似文献
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试验研究复合微生态制剂对雏鹅生长性能的影响。采用单因素随机设计,将160只1日龄健康苏牧白鹅仔鹅,随机分为4个处理,每个处理4个重复,每个重复10只,公母各半。0-3周龄雏鹅饲喂两种不同营养水平的饲料,观察其对雏鹅日增重、饲料报酬的影响。结果表明：雏鹅日增重、饲料转化率复合微生态制剂组显著高于不添加组。随着复合微生态制剂添加量的增加,肉鹅的日增重和日采食量均上升的趋势,料重比呈下降后的趋势,其中试验3组的日增重与采食量最高,但与对照组差异显著,与试验1组、试验2组差异显著（P〈0.05）;试验1组与试验2组差异不显著（P〉0.05）;料重比的变化趋势与平均日增重相同。 相似文献
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Shun Chen Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Qihui Luo Hengmin Cui Yi Zhou Yin Wang Zhiwen Xu Zhengli Chen Xiaoyue Chen Xiaoyu Wang 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):333-339
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease. 相似文献
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ZHANG Yujie SUN Ning LIU Dong YU Jing LIU Hongxiang YU Kechao REN Yanbei GAO Tianzuo YANG Shaoyan GONG Xiao ZOU Min ZHANG Yulong WANG Yuchao ZHONG Sheng MA Dong DU Yuanzhao 《畜牧兽医学报》1956,51(11):2765-2777
From October 2017 to May 2019, 67 samples of typical goslings gout were collected from Guangdong, Sichuan, Hebei, Shandong, Anhui, Liaoning provinces and other vast goose-raising areas in China. The results showed that goose astrovirus was detected in all samples and about 94.03% of the samples were infected by two different kinds of goose astrovirus. The pathogenicity of goose astrovirus FLX variant strain (named SCCD) which could stably proliferate in goose embryos and kill 10-day-old goose embryos was more virulent than goose astrovirus FLX strain. The new type of goose astrovirus strain (named SDPD) could't stably proliferate in goose embryos and SPF chicken embryos. The whole genome nucleotides homology of goose astrovirus FLX strain with goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain were 89.7% and 58.1%, the amino acids homology of ORF1b gene were 98.4% and 61.0%, the amino acids homology of ORF2 gene were 81.0% and 42.5%, respectively. The newly isolated goose astrovirus strains were inoculated into 1-day-old healthy goslings, the results showed that although the goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain could cause the death of goslings and the changes of hepatitis, kidney swelling and weight loss, none of the goslings showed typical gout characteristics (urate deposition in the organs of the body), however, 44% of goslings were characterized with typical gout which was consistent with the symptoms of natural disease after coinfected by the two goose astrovirus strains. Therefore, the pathogenic agents of goslings gout may be two different kinds of goose astrovirus, namely the new type of goose astrovirus and the variant of goose astrovirus FLX. 相似文献
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鹅星状病毒的分离鉴定及全基因组序列分析 总被引：1，自引：1，他引：0
2017年10月—2019年5月,本实验室从广东、四川、河北、山东、安徽、辽宁等广大养鹅地区采集了67份典型雏鹅痛风样品进行了实验室检测以及病原的分离鉴定,检测结果表明：所有样品中均检测到了鹅星状病毒,并且约有94.03%的样品为两个不同种的鹅星状病毒混合感染。病原分离结果表明：鹅星状病毒FLX株的变异株病毒（命名为SCCD）可以在鹅胚上稳定增殖,并且能稳定致死10日龄鹅胚,致病力较鹅星状病毒FLX增强;新型鹅星状病毒株（命名为SDPD）不能在鹅胚和SPF鸡胚上稳定增殖。病毒的基因组测序结果表明：鹅星状病毒FLX株与鹅星状病毒FLX的变异株病毒SCCD株、新型鹅星状病毒SDPD株全基因组核苷酸相似性分别为89.7%、58.1%,ORF1b基因氨基酸相似性分别为98.4%、61.0%,ORF2基因氨基酸相似性分别为81.0%、42.5%。将新分离的鹅星状病毒株接种1日龄健康雏鹅,结果表明,鹅星状病毒SCCD株和鹅星状病毒SDPD株虽然能使雏鹅发生死亡,引起肝炎、肾肿胀和增重减少等变化,但雏鹅均无典型痛风（脏器尿酸盐沉积）表现,而两种鹅星状病毒株混合攻毒能使44%的雏鹅发生典型痛风,并与临床发病症状一致。因此,临床上引起雏鹅痛风的病原可能是两种鹅星状病毒,即新型鹅星状病毒和鹅星状病毒FLX的变异株病毒。 相似文献