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1.
为探究褪黑素受体（MTNR）在牦牛子宫中的定位及表达与妊娠的关系,本研究利用免疫组织化学技术（S-P法）分别检测未妊娠、妊娠25~45 d、妊娠50~85 d、妊娠90~110 d牦牛子宫中MTNR的表达情况。结果表明：MTNR免疫阳性产物在牦牛子宫腺上皮细胞、腔上皮细胞、基质细胞、血管平滑肌细胞、血管内皮细胞、肌层平滑肌细胞均有表达;不同妊娠阶段牦牛子宫中的基质细胞和肌层平滑肌细胞中均有MTNR的表达,但其差异性不显著（P〉0.05）;血管内皮细胞和血管平滑肌细胞中的MTNR表达强度随着妊娠阶段时间的增加且差异性显著（P〈0.05）;腺上皮细胞中的MTNR在妊娠后期表达强度增加且差异极显著（P〈0.01）;腔上皮细胞的MTNR在妊娠后期表达强度降低（P〈0.01）。 相似文献
2.
甘加型藏羊发情周期阴道细胞变化规律 总被引：1，自引：0，他引：1
《中国兽医学报》2016,(7):1241-1246
采用阴道涂片法,观察了8只甘加型藏羊连续2个发情周期阴道细胞涂片,统计分析了发情周期不同时期阴道细胞种类、形态变化特点及其所占比例。结果显示:甘加型藏羊发情周期阴道涂片中主要有副基底层上皮细胞、中间层上皮细胞、表层上皮细胞、不完全角化上皮细胞、完全角化上皮细胞、白细胞和细胞碎片。发情期角化细胞所占比例最高,显著高于发情前期、发情后期和间情期(P0.05);发情前期表层上皮细胞所占比例显著高于其他三个时期(P0.05);间情期和发情后期白细胞所占比例显著高于发情前期和发情期;中间层上皮细胞在各时期所占比例差异不显著(P0.05)。结果表明:根据涂片中阴道细胞种类和各类细胞所占比例的变化特点可以简便、快速准确地鉴定和区分甘加母羊发情周期的不同阶段,阴道凃片是高效、准确的发情鉴定方法。 相似文献
3.
发情周期中山羊子宫和输卵管内肥大细胞及组织胺含量的变化 总被引：1，自引：1，他引：0
应用改良甲苯胺蓝染色法和透射电镜技术观察了山羊发情周期4个阶段肥大细胞（MC）在子宫和输卵管内的分布、形态及数量变化规律,用荧光测定改良法检测了子宫和输卵管组织中组织胺（HA）含量。结果显示：MC呈圆形、椭圆形或梭形,在子宫内主要分布在肌层,子宫外膜中零星散在,内膜中散在分布于固有层的子宫腺和血管周围,子宫阜和内膜上皮中未发现。输卵管内MC在黏膜皱襞、肌层和外膜中均有分布。MC在发情后期有明显的脱颗粒现象。发情周期中子宫及输卵管MC数量变化为：MC（发情后期）〉MC（发情前期）〉MC（间情期）〉MC（发情期）,子宫肌层MC数量各组间差异极显著（P〈0．01）,子宫固有层和输卵管内无显著变化。子宫角内HA含量在发情后期与其它时期有显著变化（P〈0．05）,子宫体、子宫颈和输卵管的变化不明显（P〉0．05）。 相似文献
4.
《中国兽医学报》2017,(10):1998-2003
为了探讨甘加藏羊发情周期腺垂体和血浆中FSH和LH含量动态变化规律及排卵的关系。选取2.5~5岁,健康未孕雌性甘加藏羊30只,采集发情周期各阶段和乏情期腺垂体和血浆,采用酶联免疫吸附试验(ELISA)测定发情前期、发情期、发情后期、间情期及乏情期腺垂体和血浆中FSH和LH的含量。结果表明,甘加藏羊发情周期内腺垂体FSH浓度在发情期(6.873IU/L)和发情后期(6.863IU/L)较低,与间情期最大(8.228IU/L)差异显著(P<0.05),发情前期又降低(8.096IU/L);腺垂体LH浓度发情期(9.39IU/L)和发情后期(9.585IU/L)较低,间情期最大(10.629IU/L),发情前期(9.7325IU/L)降低,各时期差异不显著(P>0.05);发情周期内血浆中FSH浓度在发情前期(2.599IU/L)达到峰值,其他时期均较低,发情前期与其他时期差异显著(P<0.05);血浆LH浓度在发情期(3.866IU/L)达到峰值,发情后期(3.179IU/L)和间情期(3.292IU/L)降低,各时期差异不显著(P>0.05);并且FSH和LH在发情周期内均出现4个波峰:乏情期腺垂体和血浆中FSH和LH的浓度均低于发情周期内FSH和LH各自的基础值。FSH和LH含量在垂体和血浆的这些差异性应该是两者协同调控藏羊发情和排卵的主要原因。 相似文献
5.
促卵泡素受体和促黄体素受体在猪子宫中的定位研究 总被引：6，自引：3，他引：3
为探明2种促性腺激素受体（FSHR、LHR）在猪子宫中的位置分布,本试验运用免疫组化ABC法对卵泡期、黄体期猪的子宫FSHR、LHR分别进行定位染色。结果显示,FSHR阳性细胞主要见于子宫内膜上皮细胞、血管平滑肌细胞。LHR阳性细胞主要见于子宫内膜上皮细胞、子宫肌层平滑肌细胞及血管平滑肌细胞;动情周期不同,FSHR、LHR的表达量也不同。卵泡期子宫内膜上皮细胞FSHR的表达量高于黄体期,血管平滑肌FSHR的表达量低于黄体期;卵泡期子宫血管平滑肌细胞LHR的的表达量高于黄体期,而子宫内膜上皮细胞、平滑肌细胞LHR的表达量均低于黄体期。 相似文献
6.
研究发情周期不同阶段KiSS-1和GPR54 mRNA在小尾寒羊卵巢中的表达规律,为从分子水平阐明小尾寒羊多胎性的奠定基础。将12只雌性小尾寒羊随机分为4组,分别处于发情前期、发情期、发情后期和间情期,每组3只,颈动脉放血致死后采集卵巢,采用实时荧光定量PCR技术研究发情周期不同阶段母羊卵巢上KiSS-1和GPR54基因的表达规律。结果表明:母羊发情周期各阶段卵巢上均有KiSS-1和GPR54基因表达;发情期母羊卵巢KiSS-1基因的表达量显著提高(P<0.01),比发情后期、间情期和发情前期分别提高了1.0、1.7倍和1.4倍,发情周期不同阶段GPR54基因在卵巢上的表达量无显著差异(P>0.05)。研究结果表明,发情期母羊卵巢上KiSS-1基因的表达量极显著高于发情周期其他阶段,提示卵巢上KiSS-1表达可能参与母羊排卵过程的调节。 相似文献
7.
为了研究BDNF与TrkB蛋白在小鼠发情周期和妊娠早期子宫、输卵管及着床前胚胎中的表达规律,试验采用免疫组织化学方法检测BDNF与TrkB蛋白在子宫和输卵管中的表达,采用免疫荧光技术检测着床前不同发育阶段胚胎中蛋白的表达,并利用图像分析软件对两种蛋白在着床前胚胎中的表达强度进行定量分析。结果表明:BDNF与TrkB蛋白在发情周期及妊娠3.5天和4天子宫腔上皮、子宫腺上皮和血管内皮细胞中表达,在发情周期子宫内膜固有层基质细胞中不表达,但在妊娠3.5天和4天子宫蜕膜细胞中表达,发情前期、发情期和发情后期BDNF蛋白的表达强度高于发情间期;BDNF与TrkB蛋白在妊娠2天输卵管黏膜上皮和血管内皮细胞中表达;两种蛋白在着床前胚胎各发育阶段都有表达,在囊胚期达到最高水平。说明BDNF和TrkB蛋白通过旁分泌或自分泌途径对子宫、输卵管尤其是着床前胚胎发育发挥调控作用。 相似文献
8.
《畜牧与兽医》2017,(3):5-8
用ELISA对10头发情期内和5头乏情期内的初情期母牦牛进行24 h内血清促性腺激素释放激素(GnRH)、黄体生成素(LH)、卵泡刺激素(FSH)分泌情况检测并比较体重和解剖观察子宫的发育情况。结果显示:发情期母牦牛体重、子宫重、子宫颈-子宫体长、外阴-子宫体长均高于乏情期母牦牛(P0.05),并且在发情期内发情母牦牛高于未发情母牦牛(P0.05);发情期24 h内初情期母牦牛在GnRH、LH、FSH分泌量明显高于乏情期母牦牛(P0.001);在发情期内发情母牦牛GnRH、LH、FSH的分泌量明显高于未发情母牦牛(P0.001)。对比发情期内未发情母牦牛与乏情期母牦牛,二者分泌量均较低,但是发情期内未发情母牦牛激素含量较高并且有较为明显的峰值出现。 相似文献
9.
《中国兽医学报》2016,(6):1059-1064
采用阴道涂片法鉴定大鼠所处的发情阶段,应用甲苯胺蓝染色观察肥大细胞(MC)在大鼠发情周期不同阶段子宫内的分布、形态和数量的变化规律,应用荧光分光光度计检测大鼠发情周期不同阶段子宫内组织胺(HA)的含量变化。结果显示,在大鼠发情周期中,MC在子宫内不同部位内的分布存在明显的差异,数量依次为:子宫角子宫体子宫颈。大鼠子宫各部位在发情周期各阶段内MC的数量依次为:发情后期(ME)发情前期(PE)发情间期(DE)发情期(E),子宫角内不同生理阶段MC的数量之间差异极显著(P0.01);子宫体、子宫颈内发情后期与发情期内MC数量差异极显著(P0.01),发情间期与发情前期内MC数量差异不显著(P0.05)。子宫各部位在发情周期各阶段内HA的含量依次为:发情后期(ME)发情前期(PE)发情间期(DE)发情期(E),子宫角中的HA含量在发情后期明显高于其他3个时期(P0.05),子宫体和子宫颈内HA含量在发情周期各阶段中差异均不显著(P0.05)。研究表明,根据阴道细胞类型的变化来确定大鼠发情周期的各阶段是可行的。大鼠子宫内MC的数量及形态随发情周期阶段的变化而改变,对子宫的局部免疫具有重要的作用。发情周期子宫内HA含量的变化与MC的变化一致,MC主要通过释放HA发挥调节子宫尤其是子宫角的局部免疫水平的作用,在一定程度上,可以将子宫角组织中MC的数量作为HA含量的指标。 相似文献
10.
本研究旨在探明犬子宫和卵巢在正常发情期与患子宫蓄脓时，其组织结构及乳铁蛋白（lactoferrin，LF）表达的变化特征。应用Masson’s、VVG、PAS组织化学染色方法观察乏情期、发情期、患子宫蓄脓时犬子宫及卵巢的组织结构特点，用免疫组织化学SP法观察LF的分布特征。结果显示，正常发情期犬：乏情期、发情期子宫内膜肌层厚度比分别为0.762 0、0.924 3；乏情期子宫固有层中胶原纤维含量大于发情期，发情期卵巢中胶原纤维含量大于乏情期；子宫血管层及卵巢血管内弹性膜清晰完整；子宫腺在乏情期时浅层管腔小，深层较大、腺管上皮为单层柱状上皮、上皮细胞及管腔内PAS阳性反应较强，发情期子宫腺管腔变大、腺管上皮为单层立方上皮、上皮细胞及管腔内有PAS阳性反应；子宫黏膜上皮在乏情期和发情期均为单层柱状上皮，但胞核位置不同，乏情期胞核位于中央，发情期胞核位于顶部。患子宫蓄脓犬：子宫内膜肌层厚度比为1.615 0；子宫固有层和卵巢中胶原纤维含量少于正常发情期；子宫腺管腔大，且形状不规则，管腔内有炎性细胞浸润，腺管上皮为单层立方上皮，有淋巴细胞位于基膜，上皮细胞及管腔内PAS阳性反应较弱；子宫血管层及卵巢血管内弹性膜较正常发情期变薄，且有断裂现象；子宫黏膜上皮为单层柱状上皮，胞核位于基底。LF在乏情期子宫腺上皮和卵巢中的表达水平高于发情期，而在子宫黏膜上皮中发情期的表达水平高于乏情期。患子宫蓄脓时犬子宫和卵巢中LF的表达水平均较低。综上表明，犬正常发情期与患子宫蓄脓时的子宫和卵巢组织结构特点显著不同，LF的表达水平也存在差异。 相似文献
11.
Protein and mRNA expression of follicle‐stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens) 
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下载免费PDF全文 S‐d Huo S‐e Chen R‐j Long J‐t Yang J‐x Lu R‐x Zang T‐j Zhang A. Abudureyimu J‐l Liu G‐h Zhang Y‐q Zhao Z‐r Ma 《Reproduction in domestic animals》2017,52(3):477-482
Follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) have a central role in follicle growth, maturation and oestrus, but no clear pathway in the seasonal oestrus of yak (Bos grunniens) has been found. To better understand the role of FSH and LH in seasonal oestrus in the yak, six yaks were slaughtered while in oestrus, and the pineal gland, hypothalamus, pituitary gland, and gonads were collected. Using real‐time PCR and immunohistochemical assays, we determined the mRNA and protein expression of the FSH and LH receptors (FSHR and LHR) in these organs. The analysis showed that the FSHR mRNA expression level was higher in the pituitary gland tissue compared with LHR (p < .01) during oestrus. By contrast, there was low expression of FSHR and LHR mRNA in the pineal gland and hypothalamus. FSHR mRNA expression was higher than that of LHR (p < .05) in the ovary, whereas LHR mRNA expression was higher than that of FSHR (p < .01) in the uterus. FSHR and LHR proteins were located in the pinealocyte, synaptic ribbon and synaptic spherules of the pineal gland and that FSH and LH interact via nerve fibres. In the hypothalamus, FSHR and LHR proteins were located in the magnocellular neurons and parvocellular neurons. FSHR and LHR proteins were localized in acidophilic cells and basophilic cells in the pituitary gland, and in surface epithelium, stromal cell and gland epithelium in the uterus. In the ovary, FSHR and LHR protein were present in the ovarian follicle. Thus, we concluded that FSHR and LHR are located in the pineal gland, hypothalamus, pituitary and gonad during oestrus in the yak. However, FSHR was mainly expressed in the pituitary gland and ovaries, whereas LHR was mainly expressed in the pituitary gland and uterus. 相似文献
12.
The intra-abdominal tumor developing in the uterus and lung of a domestic Shorthair cat was examined histopathologically and immunohistochemically. The tumor showed a proliferation of both endometrial stromal and smooth muscle cells accompanied by prominent vasculature. There were well-differentiated endometrial glands, and tubuli made up a monolayer of eosinophilic cuboidal epithelium. Immunohistochemically, the spindle-shaped cells and half of the stromal-like cells reacted to caldesmon and desmin antibodies. The neoplastic epithelium expressed AE1/AE3 cytokeratin. Feline endometrial stromal tumor has, to the best of our knowledge, not been reported previously and has smooth muscle and glandular components that are a unique variant to the human counterpart. 相似文献
13.
E van Engelen VNA Breeveld-Dwarkasing ME Everts GC van der Weyden MAM Taverne VPMG Rutten 《Reproduction in domestic animals》2009,44(2):303-311
The bovine cervix contains a large amount of smooth muscle cells distributed over an outer muscular layer and within a stromal layer. The stromal layer exhibits no electromyographic (EMG) activity at parturition. This leads to the question whether the stromal smooth muscle cells of the bovine cervix are prepared to contract with parturition, or whether they have another function. To this end, cervical biopsies were repeatedly taken from 10 pregnant cows at day-185 and -275 of gestation, at spontaneous, uncomplicated calving and at 30 days after calving. The smooth muscle bundles of the stroma were immunohistochemically analysed (n = 5) with regard to their integrity and cellular density, and the degree of staining for connexin-43, smooth muscle actin α (SMA), desmin and vimentin. Additionally, the mRNA expression for connexin-43, SMA, desmin and vimentin was determined with RT-PCR (n = 5). The smooth muscle tissue was arranged in bundles, also at parturition. However, the cellular density of these bundles and the SMA mRNA expression were decreased at parturition. Additionally, the SMA staining and connexin-43 expression and staining remained constant during pregnancy and at parturition. This might indicate that stromal smooth muscle cells are not prepared to contract with parturition, in contrast to the myometrial smooth muscle cells. The smooth muscle cells, stained for SMA, also expressed vimentin, and the proportion of co-expression was increased at day-275 of pregnancy. This suggests that the stromal smooth muscle cells predominantly have a secretory function in cows. 相似文献
14.
M.‐C. Madekurozwa 《Anatomia, histologia, embryologia》2013,42(1):48-56
This study describes the distribution of vimentin, desmin, smooth muscle actin (SMA) and laminin in the oviduct of the laying domestic fowl. Vimentin immunostaining was localised in the luminal epithelium of the infundibulum, magnum, magnum–isthmus junction and isthmus. The luminal epithelium of the shell gland regions displayed weak vimentin immunostaining. Vimentin immunostaining was demonstrated in the glandular grooves of the tubular infundibular region. In contrast, gland cells in the magnum, isthmus and shell gland regions were vimentin immunonegative. Fibroblasts and vascular endothelial cells in the lamina propria of the oviductal regions studied exhibited vimentin immunostaining. Strong desmin and SMA immunostaining were present in the smooth muscle cells of the tunica muscularis and vascular tunica media. In this study, basement membranes underlying the luminal and glandular epithelia were immunopositive for laminin. In addition, basement membranes associated with smooth muscle cells exhibited laminin immunostaining. The results of the study indicate that the immunolocalisation of desmin, SMA and laminin in the oviduct of the domestic fowl is similar to that in the mammalian uterus. The immunolocalisation of vimentin in the domestic fowl varies depending on the oviductal region. 相似文献
15.
Misirlioglu D Nak D Sevimli A Nak Y Ozyigit MO Akkoc A Cangul IT 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(5):225-229
The presence of oestrogen-alpha receptor (ER), progesterone receptor (PR), and HER-2/neu (c-erbB-2) oncoprotein in the uterine walls of 10 healthy cats and 20 subjects with cystic endometrial hyperplasia-pyometra (CEH-P) were evaluated. Lesions were graded according to the severity of cystic dilation, hyperplasia and inflammation, and were classified as normal, mild uterine hyperplasia and severe uterine hyperplasia. The ER, PR and c-erbB-2 expression in the endometrium, glandular epithelium, stromal fibroblasts and myometrial smooth muscle cells was quantified by immunohistochemistry. The ER, PR and c-erbB-2 staining patterns differed between normal uteri and uteri with CEH-P. The ER expression was tended to be higher in the endometrial surface and glandular epithelium in the severe hyperplasia group (P > 0.05) and significantly lower in the mild hyperplasia cases compared with normal endometrium (P < 0.05), whereas the PR expression in both severe and mild hyperplasia cases tended to be higher in stromal cells and glandular epithelium than those in the normal uteri. C-erbB-2 immunoreactivity was observed only in the endometrial surface and glandular epithelium of the uterine wall and immunostaining was found to be highest in cases with severe hyperplasia. As a conclusion, we suggest that c-erbB-2 oncoprotein may play a role in the pathogenesis of the CEH together with the ER and PR in cats, and that ER does not have a role in the mechanism of pyometra, whereas PR plays a role in the pathogenesis of both CEH and pyometra. 相似文献
16.
促性腺激素受体在雌性水牛生殖器官的表达定位研究 总被引：2，自引：1，他引：1
为研究促性腺激素受体(FSHR、LHR)在广西雌性水牛生殖器官中的分布情况,运用免疫组化SABC法对处于不同发情周期(卵泡期、黄体期)成年水牛的卵巢、子宫、输卵管中FSHR、LHR分别进行染色定位。结果表明,FSHR/LHR阳性细胞在卵巢主要见于卵巢内膜细胞及卵泡颗粒细胞;子宫主要见于子宫内膜上皮细胞和腺体细胞;输卵管主要见于柱状上皮纤毛细胞。其中,随着发情周期不同,FSHR、LHR的表达量也有所差异,卵巢中卵泡期FSHR、LHR的表达量均高于黄体期;子宫中FSHR的表达量卵泡期高于黄体期,LHR的表达量黄体期高于卵泡期;而输卵管并没有显著差异。 相似文献
17.
An ovarian mucinous cystadenoma was found in a 5-year-old female cynomolgus monkey (Macaca fascicularis). The tumor was composed of various sizes of multilocular cystic glands lined by a single layer of mucin-filled epithelium. Each of these cystic glands was surrounded by a large amount of solid fibrous stroma resembling smooth muscle. The ovarian surface epithelium showed partial invagination into the ovarian cortex, and a transition was observed between the surface epithelium and the mucinous cyst-forming epithelium. Immunohistochemically, the stromal cells were positive for alpha-smooth muscle actin and proliferating cell nuclear antigen. Ultrastructurally, the glandular epithelium had numerous mucinous secretory granules and microvilli. The stromal cells had numerous parallel microfibrils with focal density. It is rare to encounter evidence of a transition from the surface epithelium to the mucinous tumor epithelium and to show stromal smooth muscle proliferation in a mucinous cystadenoma. 相似文献
18.
应用免疫组织化学技术和图象分析方法对乏情期、怀孕期牦牛卵巢的促卵泡素受体(FSHR)、促黄体素受体(LHR)的表达特点进行了研究。结果表明,牦牛卵巢的皮质、髓质、颗粒层和膜内层、黄体中都分布有FSHR和LHR。且牦牛卵巢皮质、髓质中的FSHR光密度值在乏情期显著大于怀孕期(P0.05);怀孕期无黄体侧卵巢颗粒层和膜内层中FSHR光密度值均大于乏情期和怀孕期有黄体侧(P0.05)。怀孕期有黄体侧卵巢皮质处LHR光密度值最大,乏情期次之,怀孕期无黄体侧最小,各组间差异显著(P0.05);在怀孕期无黄体侧卵巢髓质处LHR光密度值显著小于怀孕期有黄体侧和乏情期(P0.05);在乏情期、怀孕期无黄体侧和怀孕期有黄体卵巢颗粒层和膜内层处LHR光密度值之间差异均不显著(P0.05)。表明牦牛卵巢中FSHR和LHR随着生殖阶段的不同而变化。 相似文献
19.
旨在研究RBM3在牦牛(Bos grunniens)生殖相关调控中的作用。本研究以卵泡期、黄体期和妊娠期4~6岁健康雌性牦牛各3头为采集对象，采集其卵巢、子宫和输卵管共9组试验样品，每组设置3个生物学重复。利用基因克隆技术克隆牦牛RBM3基因并进行生物信息学分析；利用实时荧光定量PCR (quantitative real-time PCR,qRT-PCR)和蛋白免疫印迹(Western blot,WB)分别在基因层面和蛋白层面检测RBM3在牦牛子宫、卵巢和输卵管中的相对表达量；利用免疫组织化学(immunocytochemistry,IHC)方法检测RBM3蛋白在试验样品中的分布定位。牦牛RBM3基因(GenBank No.:MF142258.1)被成功克隆并预测出二、三级结构，发现其与野牦牛亲缘性最近，基因编码区第51位、98位核苷酸不同于常见哺乳动物，生物信息学分析预测其编码的蛋白质为稳定的非跨膜蛋白。RBM3蛋白在本试验9组样品的表达量有如下差异：在卵巢中，黄体期显著高于卵泡期和妊娠期(P＜0.05)；输卵管中，卵泡期显著低于黄体期和妊娠期(P＜0.05)；子宫上，卵泡期显著高于黄体期和妊娠期(P＜0.05)。免疫组织化学结果显示，RBM3在卵巢中的主要表达位置是卵泡膜、颗粒层和黄体细胞；在输卵管的表达位置是黏膜上皮；子宫上的表达主要以子宫内膜和子宫腺为主。本研究结果提示，RBM3可能参与牦牛个体发情周期和妊娠过程的调控以及妊娠识别等过程，为RBM3这一冷应激调节因子参与牦牛对高原环境的适应性生理机制方面的研究提供参考。 相似文献