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1.
萝卜和甘蓝远缘杂交研究   总被引:9,自引:0,他引:9  
以4个栽培萝卜(Raphanus sativus, 2n = 18, RR)分别和芥蓝(B rassica oleracea var. alboglabra, 2n = 18, CC) 、苤蓝(B rassica oleracea var. acephala, 2n = 18, CC)杂交,研究萝卜和甘蓝的杂交亲和性。萝卜×甘蓝受精前亲 和性低,只有极少数甘蓝花粉粒能在萝卜柱头上黏附、萌发,延伸到花柱,到达胚珠。母体内杂种胚很多能发育成 熟,平均每杂交100朵花得到杂种3. 9株,说明萝卜×甘蓝生殖隔离主要发生在受精前,受精后障碍相对较轻。甘 蓝×萝卜受精前亲和性高,萝卜花粉能在甘蓝柱头上正常萌发、延伸,但采用常规的人工去雄杂交方法没有得到杂 种,而用幼胚离体培养获得了杂种,表明甘蓝×萝卜生殖障碍主要发生在受精或受精后。  相似文献   

2.
采用程氏指数对两系杂交组合亲本及其杂种F1进行分类,对杂种F1产量性状与亲本及杂种F1程氏指数,杂种F1产量性状与父本产量性状进行相关分析。结果表明,偏粳×粳、偏粳×偏粳的组合杂种F1为偏粳类型。杂种F1每穗总粒数表现与双亲程氏指数差呈显著正相关,同时也受到父本性状值的影响。  相似文献   

3.
白菜型油菜和诸葛菜远缘杂交的子房培养及杂种鉴定   总被引:3,自引:0,他引:3  
对白菜型油菜和诸葛菜正反属间杂交及杂交子房培养进行了研究,结果表明,以授粉后7 d 取子房进行离体培养其子房存活率和结籽率均较高。对于所使用的4种培养基,培养基A(MS+2.0mgL-1 6-BA+0.2 mgL-1 NAA+500 mgL-1水解酪蛋白+0.5%活性炭)显示了较好的效果,在杂交组合澧临白菜×诸葛菜中,其最高结籽率达2.56%。适宜的母本结合授粉后恰当的子房离体时间和合适的培养基成分有利于提高杂种的获得频率。通过子房培养,从杂交组合澧临白菜×诸葛菜获得的1株杂种植株,形态上显示出双亲的中间型。细胞学研究显示,该杂种小孢子和花粉发育呈现异常现象,花粉育性低。形态学和SSR分子鉴定表明,该杂种植株为真杂种。因此,通过有性杂交结合子房培养能够将诸葛菜的有益性状转入白菜型油菜中。  相似文献   

4.
为了解老芒麦与紫芒披碱草正、反交杂种F1的育性、细胞遗传学表现及育种利用潜力,对杂种F1的生育特性、染色体构型、EST同工酶等进行了分析.结果表明,正交(老芒麦×紫芒披碱草)F1 和反交(紫芒披碱草×老芒麦)F1植株的生长势均很强,正交F1株高143.2 cm、全株浅绿色,反交F1株高129.7 cm、全株灰绿色;正、反交F1的穗型均呈双亲中间型,花药呈黄色,花粉可育率0.02%~0.03%,结实率为0,说明杂种高度不育;正交F1 的花粉母细胞减数分裂中期Ⅰ(PMC MⅠ)平均染色体构型为6.90Ⅰ+14.02Ⅱ,反交F1为7.82Ⅰ+13.59 Ⅱ,减数分裂后期Ⅰ有落后染色体和染色体桥等不规则现象;亲本及其正、反交杂种F1分蘖期幼叶的EST同工酶酶带的位点、数目和强弱均存在一定差异,可作为亲本及杂种在蛋白质水平识别的重要依据.  相似文献   

5.
萝卜抗根线虫基因导入油菜的研究   总被引:2,自引:0,他引:2  
甘蓝型油菜与萝卜的属间不育杂种(ACR)经0.1%秋水仙素溶液处理杂种小苗,使杂种小苗的染色体加倍产生可育株,获得了31%可育的异源倍性植株。再用甘蓝型油菜与之回交,应用胚胎挽救技术产生了133株回交后代(BC1)的杂种株。从这些杂种株中筛选出部分抗线虫性强的杂交后代,证明通过染色体工程可以将萝卜中抗线虫的基因转移到甘蓝型油菜中。  相似文献   

6.
 利用具有早世代稳定特性的水稻(早稳)和常规品种作为主体亲本,进行了早稳×早稳、早稳×常规品种、常规品种×常规品种等三种类型的杂交。结果显示,在早稳×早稳组合和早稳×常规品种的一些组合中分别以不同频率出现早世代稳定株系,常规品种之间的杂交组合后代没有出现早世代稳定株系。水稻早世代稳定特性是早世代稳定水稻亲本所特有的,早世代稳定株系出现频率与杂交组合双亲有密切关系;早世代稳定性状既不是质量性状也不是数量性状,既不是隐性基因控制也不是显性基因控制。水稻早世代稳定特性可以通过杂交传递到F1单株,并以F2群体中的株系为传递的基本单位。稳定株系的农艺性状与相应F1单株农艺性状一致。另一方面,同一杂交组合的F2群体中,既有农艺性状整齐一致的稳定株系,又有按孟德尔分离规律的分离株系存在。初步推断水稻早世代稳定亲本中存在特有的控制因子,在杂种合子细胞分裂启动时作用于有丝分裂的开关基因,关闭有丝分裂,启动了减数分裂,体细胞减数分裂导致染色体重排,并产生纯合胚,形成了农艺性状整齐一致的F2株系。  相似文献   

7.
萝卜芥蓝异源四倍体(Raphanobrassica, RRCC, 2n = 36)与埃塞俄比亚芥(B rassica carinata, BBCC, 2n = 34)正反交,结果表明:萝卜芥蓝异源四倍体×埃塞俄比亚芥可交配率为7. 3% ,反交为64. 6%。正反交F1 植株均 呈双亲中间形态,体细胞染色体数目为35,花粉母细胞减数分裂染色体主要配构型为10Ⅱ + 15Ⅰ。62个F2 植株 和22个与埃塞俄比亚芥的回交植株的形态为中间类型、偏萝卜芥蓝异源四倍体或偏萝卜。AFLP检测到F2 和BC1 植株均含有大量双亲特异片段,聚类分析表明,这些植株独立于亲本种聚成一组。  相似文献   

8.
亚种间杂交稻籽粒充实度的遗传分析   总被引:12,自引:1,他引:12  
 利用PC311等粳型广亲和品种与扬稻4号等常规籼稻品种配组得到5个组合各18个世代的材料。测定其谷粒充实度并进行母体植株基因型和胚乳基因型联合加性-显性遗传模型分析。结果表明:1)组合之间的充实度差异明显。组合测03×扬稻4号和轮回422×3037亲本的充实度较高(平均为96.17),杂交、回交后代的充实度也较高(平均数为95.93),F1等世代充实不良的问题并不严重。组合02428×明恢63两亲本的充实度很低,但杂交、回交等世代的充实度很高,这表明在一定的遗传背景下,导入外源互补基因可提高充实度。两个充实度不高的亲本也可能获得充实度很高的杂种材料,这也说明除了“以饱攻饱”外,也存在其它提高亚种间杂种充实度的育种途径。低亲(PC311)和高亲(扬稻4号) 组合,F1代的充实度较低,其它世代的高低程度与遗传的加性模型较为吻合。低亲(PC311)×低亲(IR36)后代充实度大多数世代均较低。2)对各组合的谷粒充实度进行单个和联合的遗传分析表明,扬稻4号和测03的增效作用、IR36和PC311的减效作用比较明显,这种效应在较大程度上能为母体植株和胚乳基因型联合的加性-显性-细胞质效应模型所说明,遗传表达有较高的可信度。扬稻4号和PC311分别具有最强的增、减效基因。02428×明恢63后代的充实度表现主要由正向显性作用所致。组合PC311×IR36各世代谷粒充实度表现比较复杂,遗传决定系数较低(0.17),非遗传的变异占有较大的成份。一些组合的遗传模型的离回归测验显著,表明除了基因的加性-显性作用外,还有一些因子对充实度有影响。  相似文献   

9.
利用新选育出的质核互作型雄性不育系和筛选出的恢复系配制杂种.研究了各组合的杂种F1的中亲优势、高亲优势和对照优势。结果表明.杂种F1农艺性状和产量构成性状均存在一定的杂种优势.产量构成性状的杂种优势在不同类型组合间存在较大的差异.杂优利用在多棱大麦组合和多棱×二棱大麦组合中的应用前景更为广阔.  相似文献   

10.
浙大3号简介     
浙大3号(原名Z9087)是复合杂交组合{中棉所10号×[(中8×争31)×(250×兰5)]F7}F6中,通过耐盐性分子标记辅助育种技术而育成的丰产、稳产、优质、耐盐碱棉花新品种,2005年2月通过山西省品种审定委员会审定并定名。1选育经过1980年,用丰产稳产的棉花新品种中棉所8号为母本,以纤维品质优良的争光长绒棉争光棉31进行杂交,配制杂交组合(中8×争31);同时以中棉所3号的早熟丰产系250为母本与美国无色素腺体棉种质系兰布莱特GL-5(兰5)进行杂交,配制杂交组合(250×兰5)。得到的F1杂种于1980年冬进行南繁加代。1981年以(中8×争31)F1做母本,(250×…  相似文献   

11.
Sorghum and millet phenols and antioxidants   总被引:5,自引:2,他引:5  
Sorghum is a good source of phenolic compounds with a variety of genetically dependent types and levels including phenolic acids, flavonoids, and condensed tannins. Most sorghums do not contain condensed tannins, but all contain phenolic acids. Pigmented sorghums contain unique anthocyanins that could be potential food colorants. Some sorghums have a prominent pigmented testa that contains condensed tannins composed of flavan-3-ols with variable length. Flavan-3-ols of up to 8–10 units have been separated and quantitatively analyzed. These tannin sorghums are excellent antioxidants, which slow hydrolysis in foods, produce naturally dark-colored products and increase the dietary fiber levels of food products. Sorghums have high concentration of 3-deoxyanthocyanins (i.e. luteolinidin and apigenidin) that give stable pigments at high pH. Pigmented and tannin sorghum varieties have high antioxidant levels that are comparable to fruits and vegetables. Finger millet has tannins in some varieties that contain a red testa. There are limited data on the phenolic compounds in millets; only phenolic acids and flavones have been identified.  相似文献   

12.
种子加工、检验理论与技术现状及思考   总被引:3,自引:0,他引:3  
种子加工及检验是种子向市场流通的关键,是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实“育繁推一体化”种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中,受制于行业发展水平,种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程,提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要,在做好品种优质化繁育的基础上,分析种子加工及检验理论与技术的新方向和新需求,加强种业应用性研究,建立先进的种子质量检验体系,研制适合现代种业发展需求的种子加工设备,打造种子加工产业标准化生产体系,完善种业科学技术学科建设,促进种业产业持续健康发展。  相似文献   

13.
Summary Between 1993 and 1998 205 different potato cultivars and 1220 accessions/genotypes of wild and cultivated potato species from the IPK Genebank Gatersleben were evaluated. Parameters interesting for starch isolation and especially for the use of starch were determined. Altogether, there was a higher variability in wild potato species than in cultivated potatoes for all characteristics investigated: dry matter content, starch content, protein content, amylose content and mean particle diameter of starch granules.  相似文献   

14.
Seed processing and testing hold the key to the market circulation of seeds, and are an indispensable link in commercial breeding to endow seeds with commodity attributes. The research of seed processing and testing theory and technology is an important link in improving the seed industry chain, realizing large-scale commercial breeding and consolidating the seed industry science of ‘integration of breeding, propagation and promotion’. The scientific and technological system of seed industry in China is being formed and perfected, which is subject to the development level of the industry. The theory and technology of seed processing and testing are relatively weak. We reviewed the development of seed processing and testing theory and technology, and put forward strategies and suggestions to improve the sound development of China’s seed industry. In order to meet the needs of seed industry development and on the basis of high quality breeding of varieties, the new directions and demands of seed processing and testing theory and technology were analyzed. We will work to strengthen the applied research of seed industry, establish advanced seed quality inspection system, improve seed processing equipment suitable for the development needs of modern seed industry, establish standardized production system of seed processing industry, develop the scientific and technological disciplines of seed industry, and promote the sustainable and healthy development of seed industry.  相似文献   

15.
Summary

Arbuscular mycorrhizae were inoculated into phosphorus-deficient soil fertilized with either organic or chemical fertilizer with cucumber (Cucumis sativus L.) as the first crop and lettuce (Lactu-ca sativa L.) as the second crop but without additional fertilization and AM inoculation. AM increased dry matter and fruit yield of cucumber significantly in the unfertilized, organic-fertilized and P-deficient plants compared with the fully chemical-fertilized plants. AM inoculation increased the available phosphorus in plant and soil by around 30% for all treatments except for those chemically-fertilized. The rate of AM infection did not differ significantly among the fertilization treatments, but the infection intensity was higher in unfertilized, organic-fertilized and phosphorus-deficient treatments than chemical-fertilized treatment. The residual effects of AM-inoculated to cucumber were evident for lettuce in all pre-treatments that were unfertilized and un-inoculated for the second cropping. Without P-fertilization, neither crop could grow optimally even when the soil was inoculated with AM, suggesting that AM could not serve as a substitute for phosphorus fertilizer. However, the other beneficial effects of AM on crop growth and yield could not be fulfilled with phosphorus fertilizer.  相似文献   

16.
种子加工及检验是种子向市场流通的关键,是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实"育繁推一体化"种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中,受制于行业发展水平,种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程,提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要,在做好品种优质化繁育的基础上,分析种子加工及检验理论与技术的新方向和新需求,加强种业应用性研究,建立先进的种子质量检验体系,研制适合现代种业发展需求的种子加工设备,打造种子加工产业标准化生产体系,完善种业科学技术学科建设,促进种业产业持续健康发展。  相似文献   

17.
通过比较分析芸薹属多倍体复合种与其祖先二倍体基本种在花柱、柱头大小、角果长度、宽度、种子直径 及种子干重变化上的异同,发现多倍体复合种在这些发育性状上表现出处于两个祖先二倍体亲本之间,或只偏向 于其中一个祖先亲本。同一个多倍体品种在不同发育性状上具有不同的表现,即在某一发育性状上偏向于其祖先 亲本之一,而在另一发育性状上偏向其另一祖先亲本。  相似文献   

18.
对云南保山、德宏、普洱等地咖啡病虫害开展调查研究,结果表明:咖啡锈病(Hemileia vastatrix Berk et Br.)、炭疽病(Colletotrichum coffeanum Noack)、褐斑病(Cercospora coffeicola Berk. et Cooke)、幼苗立枯病(Rhizoctonia solani Kuhn.)、旋皮天牛(Acalolepta cervina Hope)、灭字虎天牛(Xylo  相似文献   

19.
Pea (Pisum sativum L.) and oat (Avena sativa L.) were grown as sole and mixed crops in various densities under two different tillage systems on a loess soil near Göttingen/Germany in a 2-year field experiment (2002/2003). In the conventional tillage system a mouldboard plough (CT) was used and in the minimum tillage system a rotary harrow (MT) was employed. The effect of crop density and tillage system on the grain dry matter and grain N yields, N2 fixation and soil N uptake were determined to address the following questions: (i) which mixture compositions exhibit the highest grain yields compared to the sole crops, (ii) which mixture compositions also fix a high level of N2 and leave low levels of residual inorganic soil N after harvest, and (iii) whether the intercrop advantage is influenced by the tillage system. For (i) the result in 2002 showed that the highest grain yields of both sole cropped pea and oat and intercropped pea and oat were achieved at the highest densities. In 2003, when the inorganic soil N content was higher and weather conditions were warmer and drier, grain yields were significantly higher than in 2002, but sole as well as intercropped pea and oat gave their highest grain yields at lower densities. For both years and tillage systems, the highest intercrop advantages were achieved in mixtures with densities above the optimal sole crop densities. The result for (ii) was that a distinctly higher proportion of nitrogen was derived from the atmosphere (Ndfa) by intercropped pea than by sole cropped pea. However, the uptake of soil N by intercropped pea and oat was not reduced in comparison with that of sole cropped oat as the decrease in the uptake of N from the soil by oat at lower oat densities in the mixture was compensated for by the soil N uptake of pea. Additionally, the Nmin-N content of the soil following the mixtures and sole cropped oat did not differ, especially in the deeper soil layers because oat in mixture was forced to take up more soil N from deeper layers. Therefore, the risk of soil N losses through leaching after mixtures was lower compared to sole cropped pea. The tillage system (iii) had no significant influence on grain yield and soil N uptake, but N2 fixation and the competitive ability of intercropped pea were higher under CT than with MT. An additional result was that intercropping led to a significantly increased grain N content of both pea and oat compared to the sole crops. The increase in grain N content from sole to intercrop was from 3.30 to 3.42% for pea and from 1.73 to 1.96% for oat as a mean for both years and tillage systems. The present study confirms that growing pea and oat as intercrops highlights potential economic and environmental benefits which still need to be understood in more detail in order to exploit intercropping to a greater extent.  相似文献   

20.
Soil sulphur deficiency, which is increasingly prevalent in Western Europe, lowers wheat yields, and also affects the gluten quality of the flour. Differences in S availability may change the proportion of S-poor to S-rich gliadins and glutenin subunits. This may cause unpredictable and unwanted variations in wheat quality. The combined effects of nitrogen (N) and sulphur (S) fertilisers and split application of S and N on wheat gluten quality and composition were investigated. The results revealed effects of S fertilisation on dough quality. At high N fertilisation levels significant responses to S fertilisation were found which emphasised the need for precision application of S in intensive wheat production systems. Protein fractionation by SE-FPLC showed that quality differences were associated with changing proportions of high Mr polymeric proteins. Changes in protein composition of salt soluble proteins were also confirmed by proteomics. Glyceraldehyde-3-phosphate dehydrogenase and one of the serpin protein spots increased at high N, combined with the lower S level. The enzymes also increased in samples with increased S fertilisation combined with low N, but was not changed at higher N levels. Furthermore, at high N the serpin protein spot, and also a 27 K protein and one unidentified protein spot decreased with increasing S.  相似文献   

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