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1.
应用聚合酶链反应检测禽多杀性巴氏杆菌的研究 总被引：2，自引：0，他引：2
根据基因库中禽巴氏杆菌病原的基因序列（U51470）,设计了一对跨幅为488bp的引物,并用这对引物对11个不同血清型的禽巴氏杆菌标准株、9株地方分离株和5株哺孔动物巴氏杆菌菌株及其它6种禽病病原进行了PCR扩增,结果11个血清型的禽源巴氏杆菌和9株禽巴氏杆菌地方分离株均得到了片段大小与实验设计相一致的PCR扩增产物,而对5个血清型的哺乳动物巴氏杆菌和其它6种禽病病原的扩增结果为阴性。该PCR能检出10pg的禽巴氏杆菌DNA模板。 相似文献
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为了确定在我国家禽中流行的多杀性巴氏杆菌的血清型,我们鉴定了111株,分离到的禽源菌株,其中102株是由死禽中分离到的,另9株是已致弱的菌株。在荚膜定群中,105株菌株属于A群,6株未能被定群。由我国分离的多杀性巴氏杆菌菌株第一次进行了菌体型的鉴定,发现111株菌株中110株都是05,仅1株属08。因而总的血清型:104株为5:A,6株为5:一,1株为8:A。 相似文献
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从广西各地患禽巴氏杆菌病急性死亡的鸡,鸭肝脏分离出５６株禽巴氏杆菌强毒株,经培养特性和生化特性试验鉴定,从中选出１５株典型禽巴氏杆菌菌株,Ｃａｒｔｅｒ荚膜抗原分型鉴定,１５株菌均为荚膜Ａ型,间接血凝滴度为１：１６０￣１：６４０。通过毒力和免疫原性测定,从中选择毒力较强,免疫原性较好的Ｂ２５、Ｂ２６、Ｂ２７ ３菌株进行人工致弱,其中Ｂ２６菌株在０．１％裂解全血马丁汤中,通过物理诱变方法致弱,即在传代 相似文献
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在过去一段时间,研完工作者对各种多杀性巴氏杆菌区分为型的问题实际上已开始了广泛的研究。有关多杀性巴氏杆菌血清学分类的工作结果曾指出,用与流行的巴氏杆菌病在血清学上同样型的菌株制造菌苗的必要性。不同动物的多杀性巴氏杆菌在血清学上的分类在我国尚未进行。现在为了改进生产抗巴氏杆菌病菌苗用菌种,我所进行了巴氏杆菌病茵株的血清学鉴定。由我国不同地区的牛、猪和其他种类动物和禽分离的多杀性巴氏杆菌,共计62株,用经改变的卡特氏(G.R. Carter)间接血凝试验进行了血清学分型。血清学的鉴定结果如下:所有由黄牛、水牛和耗十分离的12个菌株,属于卡特氏英膜B型,13个由猪分离的菌株中7个属于B型,4个A型,2个D型。这个结果与多数的国外研究资料结果不一致,在国外的资料中,猪的菌株是以A型和D型占优势,而在我国Carter氏B型菌是引起猪巴氏杆菌病的主要菌种;在26个禽的菌株中,除了7个菌株未能定型外,其他19个菌种是属于A型;另外由綿羊分离的两个菌株均属于B型,在4个由马分离的菌株中1个属于A型,3个属于B型,3个由兔分离的和1个由水貂分离的菌株全是Carter氏英膜A型。 相似文献
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从广西各地患禽巴氏杆菌病急性死亡的鸡、鸭肝脏分离出５６株禽巴氏杆菌强毒株，经培养特性和生化特性试验鉴定，从中选出１５株典型禽巴氏杆菌菌株。Ｃａｒｔｅｒ荚膜抗原分型鉴定，１５株菌均为荚膜Ａ型，间接血凝滴度为１∶１６０～１∶６４０。通过毒力和免疫原性测定，从中选择毒力较强、免疫原性较好的Ｂ２５、Ｂ２６、Ｂ２７３菌株进行人工致弱，其中Ｂ２６菌株在０．１％裂解全血马丁汤中，通过物理诱变方法致弱，即在传代过程中，把培养温度由３７℃逐渐提高到４５℃，每１２ｈ传１代而成功致弱，传至１２００代时，毒力显著减弱，且仍保持其良好的免疫原性和培养特性，从而获得了禽巴氏杆菌Ｂ２６－Ｔ１２００弱毒菌株 相似文献
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通过对禽源多杀性巴氏杆菌Ｐ１０５０株和Ｃ４８－１株及６株田间分离细菌的保存观察试验，表明用脱纤羊血和感染小鼠肝脾组织在－２０℃条件下，禽源多杀性巴氏杆菌可存活２年，用鲜血斜面封盖石蜡油保存法，禽源多杀性巴氏杆菌在４℃条件下可存活３个月，菌株保存前生生物学特性及毒力不发生变化，试验提供的方法对禽霍乱流行病学调查和血清分型研究具有实际意义。 相似文献
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为了探讨治疗禽霍乱的有效药物,我们就临床常用的抗生素等10种药物对分离自不同地区的78株禽多杀性巴氏杆菌以常规纸片法做了药物敏感性试验,并选取抑菌效果较好的氯霉素和呋喃妥因用试管法做了药物定量和抗菌性质测定,现将结果报告如下。材料与方法一、菌株:系从河北唐、秦地区患禽霍乱死亡病鸡实质脏器中分离的78株多杀性巴氏杆菌,均经生长特性和生化反应等项鉴定。 相似文献
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为了摸清我省若干地区野外流行的禽型巴氏杆菌的血清型,以便选择相同血清型的菌株制备菌苗,用于控制禽霍乱病的流行,我所从1978年开始,1982年截止,先后由河北省9个地区,包括30个县(市)的110个疫点上,共分离禽型巴氏杆菌158株,并加以定型。现将定型方法及结果报告如下。 相似文献
11.
Atypical Pasteurella strains producing a toxin similar to the dermonecrotic toxin of Pasteurella multocida subspecies multocida 总被引：2，自引：0，他引：2
This paper is the first report of the production of a dermonecrotic toxin by pasteurella strains that do not belong to the species Pasteurella multocida subspecies multocida. Four strains, isolated from cattle with atrophic rhinitis, were characterised phenotypically. The strains were related to pasteurellaceae, but their taxonomic position remained unclear. The strains produced a toxin that caused a haemorrhagic dermonecrosis in guinea pigs and was lethal to mice. Both effects were neutralised by an antiserum against the purified dermonecrotic toxin of P multocida subspecies multocida. Western blot analysis of culture filtrates of the bovine strains revealed a protein, with the same molecular weight as dermonecrotic toxin, which reacted with both polyclonal and monoclonal antibodies against the toxin. In an immunodiffusion test, anti-dermonecrotic toxin serum did not discriminate between the toxin of the bovine strains and the toxin of P multocida subspecies multocida. It is concluded that these atypical pasteurella strains produce a toxin that is closely related to the dermonecrotic toxin of P multocida subspecies multocida. 相似文献
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抗牛多杀性巴氏杆菌高免卵黄抗体IgY的制备及间接血凝检测方法的建立 总被引：1，自引：0，他引：1
本研究通过自制牛源荚膜血清A型多杀性巴氏杆菌灭活菌苗免疫产蛋鸡,采用卡拉胶结合硫酸铵沉淀法提取卵黄抗体IgY,并采用间接血凝方法检测抗体效价。结果表明,抗原致敏红细胞的最佳浓度是800μg/mL,免疫后第7周抗体效价达到高峰,效价为1：1024,高效价持续5周开始下降,测定提取的IgY浓度为8.258mg/mL,无菌检测及动物安全性实验表明制备的卵黄抗体安全可靠。本研究制备了抗牛多杀性巴氏杆菌卵黄抗体,为防治由荚膜血清A型多杀性巴氏杆菌所致的犊牛肺炎提供了新的手段。 相似文献
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Shivachandra SB Kumar AA Biswas A Ramakrishnan MA Singh VP Srivastava SK 《Tropical animal health and production》2004,36(8):743-750
An investigation was carried out to study the antibiotic sensitivity of avian strains of Pasteurella multocida and to select an effective antimicrobial agent for control of avian pasteurellosis in India. A total of 123 strains of P. multocida recently isolated from different avian species (chicken, duck, turkey, quail, and goose), from different regions of India were subjected to antibiotic sensitivity tests using 20 different antibiotics. Absolute resistance was observed against sulfadiazine. The studies indicated that the strains were most sensitive to chloramphenicol (73.98%), followed by enrofloxacin (71.54%), lincomycin (64.23%), norfloxacin (61.79%) and doxycycline-HCl (56.91%). The majority of the strains were found to exhibit intermediate sensitivity. Chloramphenicol was selected and suggested for treatment. Antibiogram studies also revealed the emergence of multidrug-resistant strains of P. multocida among Indian poultry. 相似文献
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猪多杀性巴氏杆菌的分离鉴定及生物学特性研究 总被引：17，自引：2，他引：17
用PCR方法配合生化鉴定，从有肺炎症状猪的肺脏及进行性萎缩性鼻炎(Progressive atrophic rhinitis，PAR)症状猪的鼻拭子中分离出66株多杀性巴氏杆菌(Pasteurella multocida，Pm)。然后做了药敏试验，并用PCR方法对这66株Pm进行分型及毒素基因的检测，用豚鼠皮肤坏死试验及小鼠致死试验对产毒素多杀性巴氏杆菌(Toxigenie Pasteurella multocida，T^ Pm)进一步鉴定。结果显示PCR鉴定与生化鉴定Pm结果完全一致；PCR分型表明有46株为D型Pm，18株为A型：Pm，1株为B型Pm，1株无法定型；有8株用PcR检测为T^ Pm；豚鼠皮肤坏死试验及小鼠致死试验对这8株T^ Pm的进一步鉴定也表明均为产毒素菌株。所鉴定的8株T^ Pm都为D型，都分离于有严重PAR症状的猪。 相似文献
16.
Expression of iron-regulated outer membrane proteins by porcine strains of Pasteurella multocida. 总被引：3，自引：0，他引：3 
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下载免费PDF全文 G Zhao C Pijoan K Choi S K Maheswaran E Trigo 《Canadian journal of veterinary research》1995,59(1):46-50
The outer membrane protein (OMP) profiles of two strains of capsular type A Pasteurella multocida isolated from the lungs of pigs with enzootic pneumonia were studied. Sarkosyl extracted OMPs from P. multocida grown under iron-restricted and iron-replete conditions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. Results showed that the iron-regulated outer membrane proteins (IROMPs) with molecular masses of 74 kDa, 94 kDa, 99 kDa and 109 kDa were expressed by strain A52, while 74 kDa, 82 kDa, 94 kDa and 99 kDa IROMPs were expressed by strain B80. Swine immune sera, obtained from pigs which were first immunized with a polyvalent P. multocida type A and type D bacterin and subsequently challenged with type A strain of P. multocida, contained antibodies against the IROMPs. These antibodies cross-reacted with the IROMPs expressed by avian strain P1059 of P. multocida. Convalescent-phase serum obtained from turkeys which survived fowl cholera, also cross-reacted with the IROMPs from porcine strains of P. multocida. These results suggested that IROMPs from porcine and avian strains of P. multocida may share common epitopes that were recognized by swine immune serum as well as turkey convalescent-phase serum. 相似文献
17.
One hundred avian Pasteurella multocida isolates recovered from cases of fowl cholera and related infections in England and Wales over a 13-year period were characterised by capsular PCR typing and analysis of outer membrane protein (OMP) profiles. Sixty-eight percent of the strains were of capsular type A, 14% were type F, 5% were type D, 4% were type B and 9% were untypable. Nineteen distinct OMP profiles (OMP-types) were identified based mainly on molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Fifty-six percent of the isolates were represented by 15 OMP-types, whereas 44% of the isolates were associated with four OMP-types. The extensive molecular mass heterogeneity of the OmpA and OmpH proteins supports previous findings that avian P. multocida strains are very diverse. Furthermore, the isolates studied were associated with different clinical symptoms and were recovered from a wide range of lesions and tissues. The high degree of strain diversity together with the wide variety of clinical symptoms suggest that certain avian strains of P. multocida are opportunistic pathogens of relatively low virulence. Strains of capsular types B, D and F, as well as the untypable isolates, were associated exclusively with specific OMP-types and represent distinct and widely disseminated clonal groups. These observations support the view that avian strains of P. multocida have a clonal population structure. Based on previous studies, the molecular mass heterogeneity of the OmpA and OmpH proteins might provide a selective advantage to P. multocida by generating antigenic variation. 相似文献
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本试验应用威特菌毒清、威特二氯异氰尿酸钠粉、戊二醛癸甲溴铵、氢氧化钠、百毒杀、过氧乙酸、复方次氯酸钠、克辽林8种消毒药物进行了对A型多杀性巴氏杆菌的杀灭效果试验和药物敏感试验,其结果：1∶2 000的威特菌毒清、1∶5 000的威特二氯异氰尿酸钠粉、1∶1 000的戊二醛癸甲溴铵、1∶100的氢氧化钠、1∶1 000的过氧乙酸和1∶100的复方次氯酸钠对A型多杀性巴氏杆菌的杀灭效果最好,在5 min内杀灭率均达100％;并且敏感性较高,其抑菌圈的直径均达20mm以上.而百毒杀和克辽林对A型多杀性巴氏杆菌敏感性不高,病菌对其具有较强的抵抗力. 相似文献
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禽巴氏杆菌C48-1外膜蛋白H基因克隆及序列分析 总被引：2，自引：0，他引：2
根据已发表的外膜蛋白基因核苷酸序列设计一对特异性引物，应用PCR方法扩增出禽巴氏杆菌5：A C48-1株的ompH全长片段，将ompH进行T—A克隆、序列测定和分析。结果表明，ompH全长1597bp，含有一个1056bp的开放性阅读框架（ORF），编码352个氨基酸，前20个氨基酸组成信号肽。与已知的15个血清型及痘苗株CU的ompH的核苷酸及其推导的氨基酸序列比较，核苷酸同源性在51．5％～98．7％之间，氨基酸同源性在39．3％～98，7％之间。氨基酸多序列比较显示，血清型特异性抗原表位位于60～80位和200～220位氨基酸处。 相似文献