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1.
This study was designed to investigate the effect of ghrelin on gastric acid secretion in weaning piglets both in vivo and in vitro. Thirty newborn piglets were selected from six litters and on 28, 35 (weaning), 38, 42 and 45d of age, respectively, one piglet from each litter was killed and the mucosal tissue from gastric fundus was collected for detecting ghrelin mRNA as well as H(+)-K(+)-ATPase mRNA expression and activity. Primary cultures of gastric mucosal cells from 5-week-old weaning piglets were challenged with 3x10(-5), 3x10(-4), 3x10(-3), 3x10(-2) and 3x10(-1)nmol/ml h-ghrelin, respectively, for 4h in order to further clarify the effect of ghrelin on gastric H(+)-K(+)-ATPase mRNA expression and activity. Ghrelin mRNA expression in gastric fundus kept stable from 28d to 42d, followed by a sudden increase on 45d, exhibiting a peak that was significantly higher than any other age groups investigated. H(+)-K(+)-ATPase activity and mRNA expression showed similar trends of increase with slightly different timing. H(+)-K(+)-ATPase mRNA expression tended to increase on 42d, while H(+)-K(+)-ATPase activity started to rise from 35d, but neither of them reached significantly higher levels until 45d. In vitro, ghrelin significantly increased H(+)-K(+)-ATPase activity of gastric mucosal cells at 3x10(-4), 3x10(-3), and 3x10(-2)nmol/ml, but augmented H(+)-K(+)-ATPase mRNA expression only at 3x10(-4)nmol/ml. The results indicate that ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity. Ghrelin acts on gastric mucosal cells to stimulate both mRNA expression and activity of H(+)-K(+)-ATPase in vitro. 相似文献
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半胱胺盐酸盐对仔猪胃黏膜细胞H+-K+-ATPase活性和生长抑素表达的影响 总被引：1，自引：0，他引：1
将分离的仔猪胃粘膜细胞分为4组，在含10％胎牛血清的DMEM高糖培养液中培养24h后，分别在新鲜的基础培养液中添加0（对照组）、0．001（低水平组）、0．01（中水平组）和0．1（高水平组）g／L的半胱胺盐酸盐，继续培养20h后收集细胞。测定细胞H^＋-K^＋-ATPase活性，同时用相对定量RT—PCR法测定细胞H^＋-K^＋-ATPase和生长抑素（SS）mRNA的相对丰度。结果表明：（1）低水平的半胱胺盐酸盐对H^＋-K^＋-ATPase的表达和活性没有影响（P〉0．05），但中、高水平的半胱胺盐酸盐显著提高了H^＋-K^＋-ATPase的表达和活性（P〈0．05），其中H^＋-K^＋-ATPase mRNA的相对丰度分别提高了36％和44％，H^＋-K^＋-ATPase活性分别提高了57％和53％。（2）低水平半胱胺盐酸盐对SSmRNA的表达没有影响（P〉0．05），中、高水平的半胱胺盐酸盐显著提高了SSmRNA的相对丰度（P〈0．05），提高幅度均为52％。以上结果提示半胱胺盐酸盐可增强体外培养的仔猪胃黏膜细胞H^＋-K^＋-ATPase的表达及活性，而这一作用可能由SS所介导。 相似文献
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Characterization of creep feed consumption and its subsequent effects on immune response, scouring index and performance of weanling pigs 总被引：8，自引：0，他引：8
K L Barnett E T Kornegay C R Risley M D Lindemann G G Schurig 《Journal of animal science》1989,67(10):2698-2708
Four trials were conducted to characterize the consumption of creep feed by nursing pigs and the effects of creep feeding (from 10 d to weaning at 28 d) on the immune response, scouring index and subsequent performance of weanling pigs. Pigs were fed a ground 20% CP corn-soybean meal-whey diet with 1.0% chromic oxide (control, 9 litters), this diet with 2.7% ovalbumin added as a dietary antigen (ovalbumin, 14 litters), or no creep feed (unexposed, 11 litters). At weaning, pigs within a litter were fed a 20% CP corn-soybean meal diet either with or without 2.7% ovalbumin. Creep-fed litters began eating at 11 d of age and disappearance of creep feed increased linearly until weaning (P less than .01). However, based on the chronic oxide coloring of the feces, total creep feed consumption was quite variable from pig to pig (13 to 194 g) and from litter to litter (107 to 1,550 g). Preweaning daily gain was similar between creep-fed and noncreep-fed litters; larger litters generally had lower daily gains (P less than .09) and less feed disappearance per pig (P less than .02). Weekly blood sampling showed that pigs fed the antigen diet had a higher (P less than .001) antibody titer to ovalbumin at 14, 21 and 28 d of age than did pigs fed the control diet or pigs unexposed to creep feed. At 56 and 63 d of age, all pigs given an ovalbumin injection at 49 d (1 ml containing 3 mg of ovalbumin) had responded (P less than .001) to injection, with the lowest titers for pigs fed the control creep diet and the highest titers for pigs fed the ovalbumin creep diet; titers were intermediate for pigs not fed creep. Regardless of preweaning or postweaning treatment, most pigs began scouring 4 to 5 d postweaning; scouring peaked at d 10 and returned to normal after d 15. Although the magnitude of difference was small, creep-fed pigs tended to scour more than pigs not fed creep (P less than .01). Postweaning performance was not influenced by preweaning treatments. 相似文献
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柴术抗激散对早期断奶仔猪生产性能和血清应激相关激素水平的影响 总被引：1，自引：0，他引：1
本试验旨在研究柴术抗激散对早期断奶仔猪生产性能和血清应激相关激素水平的影响。选用(28±2)日龄"杜×长×大"断奶仔猪48头,随机分为4个处理组,每个处理组3个重复,每个重复4头猪,分别饲喂添加0、1%、2%、3%柴术抗激散的日粮,试验期28 d。结果显示,日粮中添加2%和3%的柴术抗激散能显著提高断奶仔猪的平均日采食量和平均日增重(P＜0.05),显著降低料重比(P＜0.05),极显著降低腹泻率(P＜0.01),可显著降低35和56日龄仔猪的血清皮质醇水平(P＜0.05),显著提高35和56日龄仔猪血清生长激素、胰岛素、三碘甲状腺素、胃泌素水平(P＜0.05)。由此可知,日粮中添加柴术抗激散可有效缓解断奶应激对仔猪造成的伤害,且在日粮中添加2%的柴术抗激散效果最好。 相似文献
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高锌对早期断奶仔猪肠黏膜屏障和肠上皮细胞紧密连接蛋白表达的影响 总被引：3，自引：1，他引：2
为研究高剂量氧化锌对早期断奶仔猪肠黏膜屏障的影响并探讨其作用机理,选取54头21日龄断奶仔猪按胎次一致、体质量相近和公母各半方法分为3组,每组3个重复,每个重复6头.哺乳组仔猪继续哺乳至35日龄,基础日粮组饲喂基础日粮(110 mg·kg~(-1)锌),高锌组饲喂基础日粮+3 000 mg·kg~(-1)锌(氧化锌).分别于28和35日龄取样测定血浆D-乳酸、内毒素含量和二胺氧化酶(DAO)活性、肠系膜淋巴结细菌移位率以及结肠内容物大肠杆菌数,并分析回肠上皮细胞紧密连接蛋白Occludin和ZO-1的表达.结果显示:与哺乳组相比,断奶仔猪饲喂基础日粮,28日龄时血浆中D-乳酸、内毒素含量和DAO活性显著升高(P<0.05),至35日龄时,肠黏膜仍维持较高的通透性;添加高锌抑制上述3个指标的升高,进而阻止肠黏膜通透性的增加,达到与哺乳组相同的效果;与哺乳组相比,基础日粮组和高锌组在28和35日龄时均存在肠系膜淋巴结细菌移位现象,但是高锌组低于基础日粮组(P>0.05);28日龄时,基础日粮组结肠内容物大肠杆菌数大于哺乳组(P<0.05),与高锌组比较差异不显著(P>0.05),35日龄时各组间差异不显著(P>0.05);与哺乳组相比,仔猪断奶后饲喂基础日粮使Occludin和ZO-1mRNA丰度以及ZO-1的表达下降(P<0.05),高锌组Occludin和ZO-1 mRNA丰度、ZO-1蛋白表达均显著高于基础日粮组(P<0.05).结论:仔猪断奶后肠黏膜通透性增加,肠上皮细胞紧密连接蛋白表达下降;而添加高剂量氧化锌可抑制细菌在肠道黏膜上的黏附,阻止上皮细胞渗透性增加,能保护肠黏膜屏障功能. 相似文献
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本试验旨在研究酪酸梭菌对断奶仔猪生长性能、肠道组织形态及肠道通透性的影响。试验选用21日龄断奶、体重(5.35±1.33)kg的"杜×长×大"三元杂交仔猪18头,随机分为3组,分别饲喂基础饲粮(对照组)、基础饲粮+500 mg/kg酪酸梭菌(酪酸梭菌组)、基础饲粮+3 000 mg/kg氧化锌(氧化锌组),每组6个重复,每个重复1头猪,试验期14 d。结果表明:1)各组断奶仔猪平均日增重与料重比无显著差异(P0.05);酪酸梭菌组和氧化锌组仔猪腹泻率与对照组相比显著降低(P0.05)。2)与对照组相比,酪酸梭菌组和氧化锌组断奶仔猪回肠隐窝深度显著降低(P0.05),绒腺比极显著升高(P0.01)。3)酪酸梭菌组和氧化锌组断奶仔猪第14天血清内毒素与D-乳酸浓度均显著低于对照组(P0.05),酪酸梭菌组与氧化锌组之间无显著差异(P0.05)。4)与对照组相比,酪酸梭菌组和氧化锌组断奶仔猪回肠和结肠咬合蛋白(Occludin)mRNA表达量显著升高(P0.05);与氧化锌组相比,酪酸梭菌组断奶仔猪回肠和结肠闭合小环蛋白-1(ZO-1)、Occludin mRNA表达量无显著差异(P0.05)。综合以上结果,断奶仔猪饲粮中添加酪酸梭菌可以显著降低断奶仔猪腹泻率,改善肠黏膜形态,降低肠黏膜通透性,上调紧密连接蛋白的表达量,可达到与添加氧化锌相同的效果。 相似文献
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为研究日粮中不同那西肽与硫酸粘杆菌素水平及其组合对断奶仔猪生产性能的影响,笔者开展了本试验。试验采用2×2双因子试验设计,试验期为28d。试验结果表明：随着饲粮中那西肽添加量由2．5mg／kg增加到5．0mg／kg。仔猪平均日增重和平均日采食量均得到显著改善（P〈0．05）,但对饲料转化率和腹泻率的影响不显著（P〉0．05）;硫酸粘杆菌素对仔猪平均日增重、平均日采食量、饲料转化率和腹泻率的影响均不显著（P〉0．05）;那西肽和硫酸粘杆菌素未体现出显著的组合效应（P〉0．05）。 相似文献
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Effect of creep feeding on structural and functional changes of the gut of early weaned pigs 总被引：4，自引：0，他引：4
Dose-dependent hypersensitivity responses mounted against antigenic constituents of creep feeds have been previously implicated in intestinal damage, digestive disorders and impaired performance of early weaned pigs. In the present study biochemical and histological investigations of the small intestine were undertaken to determine the putative relationship between creep feed level and hypersensitivity response in pigs weaned at 14 days. Creep feed was administered by gastric intubation and weaned pigs received the same diet by gastric intubation for five days. Mucosal structure and function at weaning were not affected by creep feeding regimen. Villus height decreased and crypt depth increased in weaned compared to suckled pigs, but morphology was unaffected by prior exposure to creep. Xylose absorption was not influenced by either creep feed level or weaning. Intraepithelial lymphocyte numbers increased during weaning but were not affected by the creep feeding regimen. Mucosal carbohydrase activity in weaned pigs was not significantly affected by preweaning treatment. The results do not support the hypothesis that post weaning changes in structure and function of the gut are related to creep feed level or to immune responses to dietary antigens. 相似文献
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研究流食与颗粒料不同方式对断奶仔猪增重和采食量,选择杜长大三元杂种28日龄仔猪,随机区组设计,共六个区组,每窝一个区组,每个区组四头猪,分为处理组2头和对照组2头。28日龄体重:试验组7.19±0.027 Kg,对照组7.19±0.031 Kg。处理组和对照组均自由采食,自由饮水,35日龄晨称个体重,结果给予流食比使用颗粒料提高仔猪采食量33g/d(P<0.05)和日增重17.6g/d(P<0.05)。仔猪早期断乳是提高母猪生产力和规模化养猪业中的关键技术之一,现代规模养猪业已把乳猪断奶日龄提至21~28 d。 相似文献
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Bruininx EM Binnendijk GP van der Peet-Schwering CM Schrama JW den Hartog LA Everts H Beynen AC 《Journal of animal science》2002,80(6):1413-1418
To assess the effects of creep feed consumption on individual feed intake characteristics and performance of group-housed weaned pigs, 16 litters (149 piglets) were fed a commercial creep feed (3,040 kcal NE/kg, 15.2 g lysine/kg) supplemented with 1% chromic oxide. Another five litters (48 piglets) were not given access to creep feed (no-feed). Piglets were weaned at 28 d after birth. On d 18, 22, and 27 of age, fecal samples from all the piglets were taken using fecal loops. A green color of the feces indicated that the piglet had eaten creep feed. Piglets that had green-colored feces three times were considered as eaters. Piglets that never showed green-colored feces were considered as non-eaters. At weaning 22 piglets of each type (no-feed, non-eaters, and eaters) were selected based on BW, litter origin, and sex. These 66 pigs were assigned to six pens equipped with computerized feeding stations. Eaters, non-eaters, and no-feed pigs were equally divided over all six pens. After weaning a prestarter (d 0 to 13) and a starter diet (d 14 to 34) were offered for ad libitum consumption. The individual feed intake characteristics of latency time (interval between weaning and first feed intake) and initial feed intake (intake during the first 24 h following first feed intake) and performance traits were determined for all piglets. The pigs that were designated as eaters needed less time between weaning and first feed intake than the pigs that were designated as non-eaters and no-feed pigs (P = 0.04 and P = 0.06, respectively). Initial feed intake was not affected (P > 0.1) by feed intake prior to weaning. However, during d 0 to 8 the eaters had more visits per day during which feed was consumed than both the non-eaters and no-feed pigs. Averaged over the first 8 d after weaning, the ADFI and ADG of the eaters were higher than that of the non-eaters and no-feed pigs (P < 0.05). Averaged over the total 34-d period the effect of creep feed intake on postweaning ADFI was much less pronounced (P = 0.20), whereas ADG of the eaters was the highest (P < 0.05). Creep feed intake during the sucking period stimulates early postweaning feed intake as well as postweaning performance. 相似文献
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《中国畜牧杂志》2017,(3)
本试验旨在研究中草药添加剂对断奶仔猪肠道菌群和生产性能的影响。选择72头体重(8.13±0.67)kg的健康28日龄的三元断奶仔猪,遵照单因素原则分为4组,每组3个重复,对照组饲喂基础日粮,试验组分别在基础日粮中添加0.5%、1.0%、2.0%中草药添加剂,预试期为7 d,正式期25 d。结果表明:与对照组相比,试验组仔猪日增重、日采食量均显著增加(P<0.05),试验组耗料增重比分别显著降低26.01%、40.81%、30.49%(P<0.05);试验组仔猪大肠埃希氏菌数、腹泻率均显著低于对照组(P<0.05),B组最低;试验组仔猪乳酸杆菌和双歧杆菌数均显著增加(P<0.05);试验A、C组的各测定指标差异均不显著(P>0.05)。综合各项指标可知,中草药添加剂使断奶仔猪的生长性能显著提高,腹泻率降低,抑制肠道内大肠埃希氏菌繁殖,促进有益菌的增殖,且添加量为1%时效果最好。 相似文献
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为了研究新型发酵豆粕对断奶仔猪生长性能和胃肠道发育的影响,根据胎次、体重相近原则,选用26日龄断奶体重为(4.75±0.38)kg的90头杜枫姜仔猪分为对照组、试验Ⅰ组(日粮+17.5%发酵豆粕)和试验Ⅱ组(日粮+35%发酵豆粕),每组3个重复,每个重复10头,试验为期20 d。结果:与对照组相比,试验Ⅰ(P<0.05)、Ⅱ组(P<0.01)仔猪日增重效果均显著;料重比分别降低4.21%(P>0.05)、19.47%(P<0.05);腹泻率均显著低于对照组;两试验组仔猪胃内容物pH值均显著降低(P<0.01);而胃黏膜厚度及胃壁厚度显著增加(P<0.01)。试验Ⅱ组的肠绒毛高度、绒毛高度/隐窝深度比值显著高于对照组(P<0.05),隐窝深度和肠壁厚度显著降低;试验Ⅰ组肠道发育也有所改善。表明新型发酵豆粕可提高断奶仔猪生长性能,降低腹泻率,促进仔猪胃肠道发育,且完全替代普通豆粕时效果最佳。 相似文献
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[Objective] The paper was to compare the effects of traditional granulating process and post-expanding lower temperature granulating process on starch gelatinization degree and in vitro protein digestibility of conversation feed, and growth performance of piglets. [Method] Sixty weaned piglets, 25 days old, were randomly divided into two groups, six replicates each group, and each replicate contained five piglets. Piglets in control group were fed with the conversation feed developed by traditional granulating process, and those in experimental group were fed with the conservation feed developed by post-expanding lower temperature granulating process. The trial lasted 28 d. [Result] Compared to traditional granulating process, post-expanding lower temperature granulating process significantly enhanced starch gelatinization degree of conservation feed by56.9%(P0.05), and significantly improved in vitro protein digestibility of conservation feed by 11.48%(P0.05). Compared to the control group, the feed intake of piglets in experimental group was increased by 21.63%(P0.05); the average daily gain was increased by 27.61%(P0.05); the diarrhea rate was lowered; and the feed gain ratio was improved by 4.51%(P0.05). The results suggested that post-expanding lower temperature granulating process effectively enhanced feed digestion and absorption of piglets. [Conclusion] The paper provides a theoretical basis and practical reference for improvement of production process of conservation feed. 相似文献
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本文旨在研究丙氨酰谷氨酰胺(Ala-Gln)对早期断奶仔猪生长性能、血液生化指标和小肠黏膜酶活性的影响,探讨Ala-Gln在早期断奶仔猪饲粮中的适宜添加量.选用48头(公母各占1/2)纯种云南撒坝仔猪为试验对象,35日龄断奶,按单因素完全随机化设计分为4组,每组3个重复,每个重复4头猪.各组分别饲喂在基础饲粮上添加0(对照组)、0.15％、0.30％、0.45％ Ala-Gln的试验饲粮,试验期为35 ～ 60日龄.结果表明:1)0.30％组显著提高了全期仔猪平均日增重和平均日采食量(P＜0.05),随着Ala-Gln添加水平的提高,全期仔猪平均日增重和平均日采食量以二次曲线方式提高(P=0.004和P=0.006);2)0.30％组极显著提高了49日龄仔猪血清总蛋白、葡萄糖含量及谷丙转氨酶活性(P＜0.01),显著提高了血清碱性磷酸酶活性(P＜0.05),极显著降低了血清尿素氮含量(P＜0.01),显著降低了乳酸脱氢酶活性(P＜0.05).各组血清白蛋白、肌酐含量及肌酸激酶活性均差异不显著(P＞0.05).3)试验组仔猪空肠黏膜钠泵(Na+ -K+ -ATPase)和总二糖酶活性均显著或极显著高于对照组(P ＜0.05,P＜0.01).结果提示,添加适量的Ala-Gln对早期断奶仔猪具有一定的促生长效应,在本试验条件下,依据二次曲线拟合结果,推荐Ala-Gln适宜添加量为0.27％. 相似文献
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在日粮中添加葡萄糖氧化酶和植物血凝素防治仔猪早期断奶腹泻症的试验 总被引：1，自引：0，他引：1
[目的]研究在日粮中添加葡萄糖氧化酶和植物血凝素防治仔猪早期断奶腹泻症的效果,探讨其对仔猪生产性能和肠道健康等方面所产生的作用。[方法]选用120头断奶仔猪,随机分为对照组、试验组Ⅰ和试验组Ⅱ3组,分别饲喂常规断奶仔猪基础日粮、添加0.5%的葡萄糖氧化酶日粮以及添加0.5%的葡萄糖氧化酶和0.1%植物血凝素日粮,采用统一的饲养管理模式。记录每日腹泻次数、采食量、死亡数以及试验开始和结束时每头仔猪空腹体重;计算平均日增重、腹泻率、平均日采食量、饲料转化率及死亡率。[结果]试验期内仔猪平均日增重,试验组Ⅰ和试验组Ⅱ分别比对照组提高25.0%和26.8%,差异极显著(P<0.01);仔猪料肉比,试验组Ⅰ和试验组Ⅱ分别比对照组下降17.2%和18.7%,差异极显著(P<0.01);仔猪腹泻率,试验组Ⅰ和试验组Ⅱ分别比对照组下降56.0%和65.5%,差异极显著(P<0.01);仔猪成活率,试验组Ⅰ比对照组提高7.4%,差异不显著(P>0.05),试验组Ⅱ比对照组提高13%,差异极显著(P<0.01)。[结论]在日粮中添加葡萄糖氧化酶和植物血凝素可明显提高饲料转化效率和仔猪成活率,明显降低仔猪腹泻率。 相似文献
18.
高铜对断奶仔猪生长性能及血清激素水平的影响 总被引：5，自引：0，他引：5
选用60头28日龄±2日龄“长大定”三元杂交断奶仔猪,按体重和性别随机分成3组,每组20头。Ⅰ组(对照组)仔猪饲喂基础日粮;Ⅱ组仔猪在断奶后14d内饲喂基础日粮 250mg/kg铜(CuSO4.5H2O),然后再饲喂基础日粮;Ⅲ组仔猪饲喂基础日粮 250mg/kg铜(CuSO4.5H2O)。试验期为28d。所有实验猪均于断奶后0,7,14d和28d称重,计算采食量,观察断奶后腹泻情况。同时每组固定10头仔猪,于断奶后0,7,14d和28d,经前腔静脉采血,测定部分血清激素水平。结果表明,高铜日粮能显著提高断奶仔猪体重(AW)、平均日增重(ADG)和日采食量(ADFI),降低料重比(F/G)和断奶后腹泻率,显著提高断奶仔猪血清GHI、NS、T3和T4的水平。 相似文献
19.
20.
Zhong X Zhang XH Li XM Zhou YM Li W Huang XX Zhang LL Wang T 《Journal of animal science》2011,89(11):3634-3642
The objectives of this study were to determine the effects of oral Gln supplementation on growth performance, intestinal morphology, and expression of heat shock protein (Hsp) 70 in weaning piglets. A total of 65 piglets after weaning at 21 d of age (d 0) were used in this experiment. Five piglets were randomly selected and euthanized initially at d 0 to determine baseline values for the expression of Hsp70 in the small intestine. The remaining piglets were randomly assigned to 1 of 2 treatments and received 0 or 1 g of oral Gln/kg of BW every 12 h. After piglets were humanely killed at d 3, 7, and 14 postweaning, the duodenum, jejunum, and ileum of piglets were sampled to evaluate intestinal morphology and the expression and localization of Hsp70. The results indicated that oral Gln supplementation increased plasma concentrations of Gln compared with those in control piglets (P < 0.05). Average daily gain and ADFI were greater in piglets orally supplemented with Gln than in control piglets during the whole period (P < 0.05). The incidence of diarrhea in piglets orally supplemented with Gln was 24% less than (P = 0.064) that in control piglets at 8 to 14 d after weaning. The weights of the jejunum and ileum were greater in piglets orally supplemented with Gln compared with those of control piglets relative to BW on d 14 postweaning (P < 0.05). The villus height and the villus height:crypt depth ratio in the jejunum and the ileum were greater in piglets receiving oral Gln on d 14 postweaning (P < 0.05) than in control piglets. These results indicate that Gln supplementation can influence the intestinal morphology of weaned piglets. The expression of hsp70 mRNA and Hsp70 proteins in the duodenum and jejunum was greater in piglets supplemented with Gln than in control piglets (P < 0.05). However, Gln supplementation had no effect on the expression of hsp70 mRNA and Hsp70 proteins in the ileum. Moreover, the localization of Hsp70 in the cytoplasm indicated that Hsp70 has a cytoprotective role in epithelial cell function and structure. These results indicate that Gln supplementation may be beneficial for intestinal health and development and may thus mitigate diarrhea and improve growth performance. The protective mechanisms of Gln in the intestine may be associated with the increase in Hsp70 expression. 相似文献