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1.
The effect of density on growth in body mass (BM) and total length (TL) of Caiman latirostris hatchlings raised in small pens (area 0.36 m2), for 3 months, was investigated. Twelve animals from each of three clutches were randomly distributed (1, 2 or 3 individuals) to two replicates at low rearing density (LRD: 0.12 m2/individual), medium rearing density (MRD: 0.06 m2/individual) and high rearing density (HRD: 0.04 m2/individual. Growth at LRD [BM: 150.75 ± 43.83 g (SE); TL: 11.58 ± 0.93 cm] and MRD (BM: 141.96 ± 22.55 g; TL: 8.38 ± 1.26 cm) was significantly more than at HRD (BM: 57.19 ± 14.38 g; TL: 4.61 ± 0.89 cm; p < 0.001). Density is important to achieving optimal growth and maintaining the well-being of C. latirostris in commercial ranching programs.  相似文献   

2.
The objective was to compare water quality and fish growth and mortality in a pilot scale recirculating system (RS) and a control tank in flow through system (FTS). The RS was designed after the Danish Model Trout Farm and operated with a make up water renewal rate of 9 m3 kg-1 of fish produced. RS water quality did not decrease significantly with water flow rate decrease in the RS. During the experiment, the RS water treatment system presented solids removal efficiency of 59.6 ± 27.7% d−1, ammonia oxidation of 45 ± 32 g m−3 d−1, oxygenation yield of 392 ± 132 g of O2 kWh−1 and CO2 degassing of 23.3 ± 11.9% pass−1. In the RS, nitrite concentration was 0.15 ± 0.07 mg l−1, close to the toxicity threshold; a N2 supersaturation phenomenon was measured, probably due to the air injection depth. The biofilter and sedimentation area management has to be improved to avoid organic matter decomposition and release of dissolved elements. Even if no N2 over-saturation apparent effect on fish performance and aspect were detected, the airlift depth has to be modified in the case of industrial development of the RS. Some improvements of the water treatment system, especially on the airlift and sedimentation area, are suggested.Concerning fish growth, no significant differences were observed between the RS and the FTS. No pathologies were detected and cumulative mortality rates (0.1%) were similar to the farm's usual data. There were no significant effects of water flow rate decrease in the RS on fish performance and energy savings were recorded to be 0.7 kWh kg−1 of fish produced between RS1 and RS2. The global energy cost of the RS was 3.56 kWh kg−1 of fish produced (0.107 € kg−1 of fish produced). Even if the energy consumption of the water treatment system can be improved, the results confirm that recirculating system can be used for industrial trout on growing, without fish performance deterioration.  相似文献   

3.
Seawater transferred under-yearling Atlantic salmon (120 g) were fed a practical fishmeal formulated diet containing 57.4% blue whiting fishmeal and providing 15 g kg− 1 total phosphorous (P) in the diet. The basal diet was supplemented with graded dietary levels of inorganic P (0, 3, 6, 9, 12 and 15 g kg− 1) to total P levels of 15, 18, 21, 24, 27 and 30 g kg− 1. Within a feeding period of 12 weeks, classical signs of P deficiency developed in Atlantic salmon fed the P un-supplemented diet containing 15 g kg− 1 total P of natural origin. Deficiency signs included reduced growth and feed efficiency, reduced tissue mineral concentration (P, Ca, Mg, Zn), increased P:Ca ratio in the body, increased condition factor, metabolic disorder as indicated by lipid accumulation and impaired protein utilization, as well as reduced lipid, energy and Zn digestibility. Supplementation of inorganic P-salts in the diet at 3 g kg− 1 (18 g kg− 1 total P) significantly improved the production results with respect to growth and feed efficiency, and restored all other signs of P deficiency in fish. A higher supplementation level of 6 g kg− 1 inorganic P (21 g kg− 1 total P) was required for optimal mineralization in whole body and vertebrae. The highest supplementation levels of 15 g kg− 1 added inorganic P (30 g kg− 1 total P) seemed to increase P excretion and to have slightly negative impacts on growth and bone mineralization. Results suggest that P availability from blue whiting fishmeal is very low (3.2 g kg− 1) and inadequate to meet dietary P requirement in rapidly growing under-yearling salmon immediately following seawater transfer, despite high natural P content in the blue whiting fishmeal.  相似文献   

4.
Understanding the role of salinity in breeding and growth has the potential to enhance production of giant freshwater prawn, Macrobrachium rosenbergii. This study investigated the reproduction of females reared in salinities of 0, 6, 12, and 18 g L− 1. Mean weight of females decreased with increased salinity (31.40 ± 1.54, 25.14 ± 1.16, 20.80 ± 0.81, and 16.62 ± 1.04 g at 0, 6, 12, and 18 g L− 1, respectively). Larval production was delayed by 2 months in females reared in 12 g L− 1 compared to 6 and 0 g L− 1 and the cumulative number of berried females decreased with increased salinity. The number of larvae produced was positively correlated to weight of female. A larval production not only differed between salinity treatments, but also that larval production per gram of female differed between treatments. Females reared in lower salinity of 0 and 6 g L− 1 produced larger numbers of larvae (12,155 ± 480 and 6519 ± 323, respectively) compared to 12 and 18 g L− 1 (3751 ± 256 and 0, respectively). The number of larvae produced per gram of female was inversely related to the salinity levels (Y = − 37.54X + 685.65, n = 339, r2 = 0.995, p < 0.05). Survival of larvae from females reared at 0 and 6 g L− 1 was higher than those from females reared in 12 g L− 1. This study clearly shows that female broodstock reared in lower salinity was larger, reproduced early, and produced more offspring than at higher salinity and this could significantly impact coastal prawn culture where seasonal fluctuation of salinity in the hatchery is common.  相似文献   

5.
Effect of apidaecin on common carp (Cyprinus carpio) based on growth performance, feed utilization (Feed Conversion Ratio, FCR) and immune parameters were investigated. Twelve aquaria with three replicates for G1-3 and control were used. The synthesized apidaecin peptide was added to common carp basal diets (Control) as additives in three concentrations: 10.0 mg kg− 1 (G1), 15.0 mg kg− 1 (G2) and 30.0 mg kg− 1 (G3) by wet weight of basal diet. After an 80-day feeding experiment, G2 and G3 showed significantly better (P < 0.05) results of growth performances and FCR than the control. However, there was no remarkable difference (P > 0.05) between G2 and G3. As for G1, the final weight, SGR (Specific Growth Rate) and DG (Daily Gain) were increased and the FCR was decreased, but not significantly (P > 0.05) compared with the control. The serum lysozyme activity of G2 and G3 was significantly increased (P < 0.05) than the control. As for the alternative complement pathway activity, G2 and G3 were also higher (P < 0.05) than the control. However, no significant difference (P > 0.05) was found whichever between G2 and G3 or between G1 and G2 in both lysozyme and the alternative complement pathway activities. Furthermore, there was no remarkable difference between G1 and the control in immune parameters.  相似文献   

6.
Impact of varied durations of aeration on growth, survival and production performances of catla (Catla catla), rohu (Labeo rohita), fringe lipped carp (Labeo fimbriatus) and olive barb (Puntius sarana) were evaluated in outdoor concrete tanks (10 m × 5 m × 1.3 m) during fingerlings rearing. At a combined stocking density of 0.5 million fry/ha, provision of night time aeration for 4, 8 and 12 h was evaluated as the three treatments, T-I, T-II and T-III, respectively against control (T-c, without aeration). Overall survival and net biomass of the species increased among the treatments significantly as a function of aeration hours (T-c < T-I < T-II < T-III). Aeration for 8 and 12 h showed significantly higher survival, harvested body weight and SGR in all the carps than those with 4 h (T-I) and no aeration (T-c), suggesting 8–12 h of aeration requirement for fingerlings rearing at such high density. Further, no significant difference between survival levels in T-II and T-III groups suggested 8 h of aeration to be adequate. However, while species-wise performance showed 8–12 h night time aeration to be advantageous for catla and rohu, aeration for 4 h was adequate for fringe lipped carp and olive barb.  相似文献   

7.
Aquafeed production faces global issues related to availability of feed ingredients. Feed manufacturers require greater flexibility in order to develop nutritional and cost-effective formulations that take into account nutrient content and availability of ingredients. The search for appropriate ingredients requires detailed screening of their potential nutritional value and variability at the industrial level. In vitro digestion of feedstuffs by enzymes extracted from the target species has been correlated with apparent protein digestibility (APD) in fish and shrimp species. The present study verified the relationship between APD and in vitro degree of protein hydrolysis (DH) with Litopenaeus vannamei hepatopancreas enzymes in several different ingredients (n = 26): blood meals, casein, corn gluten meal, crab meal, distiller's dried grains with solubles, feather meal, fish meals, gelatin, krill meals, poultry by-product meal, soybean meals, squid meals and wheat gluten. The relationship between APD and DH was further verified in diets formulated with these ingredients at 30% inclusion into a reference diet. APD was determined in vivo (30.1 ± 0.5 °C, 32.2 ± 0.4‰) with juvenile L. vannamei (9 to 12 g) after placement of test ingredients into a reference diet (35 g kg− 1 CP; 8.03 g kg− 1 lipid; 2.01 kcal g− 1) with chromic oxide as the inert marker. In vitro DH was assessed in ingredients and diets with standardized hepatopancreas enzymes extracted from pond-reared shrimp. The DH of ingredients was determined under different assay conditions to check for the most suitable in vitro protocol for APD prediction: different batches of enzyme extracts (HPf5 or HPf6), temperatures (25 or 30 °C) and enzyme activity (azocasein): crude protein ratios (4 U: 80 mg CP or 4 U: 40 mg CP). DH was not affected by ingredient proximate composition. APD was significantly correlated to DH in regressions considering either ingredients or diets. The relationships between APD and DH of the ingredients could be suitably adjusted to a Rational Function (y = (a + bx)/(1 + cx + dx2), n = 26. Best in vitro APD predictions were obtained at 25 °C, 4 U: 80 mg CP both for ingredients (R2 = 0.86; P = 0.001) and test diets (R2 = 0.96; P = 0.007). The regression model including all 26 ingredients generated higher prediction residuals (i.e., predicted APD − determined APD) for corn gluten meal, feather meal, poultry by-product meal and krill flour. The remaining test ingredients presented mean prediction residuals of 3.5 points. A model including only ingredients with APD > 80% showed higher prediction precision (R2 = 0.98; P = 0.000004; n = 20) with average residual of 1.8 points. Predictive models including only ingredients from the same origin (e.g., marine-based, R2 = 0.98; P = 0.033) also displayed low residuals. Since in vitro techniques have been usually validated through regressions against in vivo APD, the DH predictive capacity may depend on the consistency of the in vivo methodology. Regressions between APD and DH suggested a close relationship between peptide bond breakage by hepatopancreas digestive proteases and the apparent nitrogen assimilation in shrimp, and this may be a useful tool to provide rapid nutritional information.  相似文献   

8.
We held juvenile big-headed turtles, Platysternon megacephalum, from eastern China, at temperatures from 20 to 29.4 °C to determine effects on feeding, growth and food conversion. Food intake increased significantly from 20 to 22.4 °C, remained high until 27.1 °C, and then decreased dramatically at 29.4 °C. Digestive efficiency for energy decreased as temperature increased, whereas the digestive efficiency of protein increased from 20 to 25 °C, and decreased at higher temperatures. The relationships between specific growth rate (SGR), food conversion coefficient (Cc) and temperature (T) were curvilinear, and could be described by quadratic equations: SGR = −0.01 T2 + 0.47 T − 5.24 and Cc = −0.37 T2 + 17.20 T − 181.85. Maximum growth was estimated to occur at 23.9 °C, with 90% of the maximum being achieved within the range of 21.9–25.8 °C; maximal food conversion occurred at 23.2 °C, with a 90% range from 21.0 to 25.4 °C. The temperature range (22–25 °C) found to promote best growth and food conversion in juvenile P. megacephalum is lower than for many other freshwater turtles. Temperatures of 22–25 °C are recommended for use in culture of this species to maximize growth and food conversion.  相似文献   

9.
The aim of this study was to non-invasively determine fat and pigment concentrations in salmon muscle based on visible and near infrared (VIS/NIR) spectroscopy measurements of live/whole fish and fillets, and by means of digital photography (DP) of fillets. The fish used were two populations of farmed Atlantic salmon (Salmo salar L.) consisting of 46 salmon averaging 0.7 kg (range 0.17–1.7 kg, Group S) and 30 salmon averaging 2.3 kg (range 1.4–4.1 kg, Group L). Chemical analyses (fat and pigment content) and computerized tomography, CT (fat content) were used as reference methods. Group L was analysed in the live state (VIS/NIR), after gutting (VIS/NIR and CT), and as fillets (VIS/NIR and DP). Group S was analysed in the gutted state (VIS/NIR) and as fillets (VIS/NIR and DP). VIS spectroscopy predictions of pigment in whole salmon from Group S were obtained with a root mean square error of prediction (RMSEP) of 0.9 mg kg− 1 astaxanthin, and a correlation between VIS spectroscopy predicted and chemically measured pigment of r = 0.85 (p < 0.0001). The fat concentration was determined by the NIR spectroscopy in live fish with RMSEP = 1.0 fat% unit, and a correlation with chemical reference values of r = 0.94 (p < 0.0001). Fat predictions from NIR spectroscopy correlated also well with predictions from CT analyses, r = 0.95 (p < 0.0001). VIS spectroscopy and DP were equally well suited to determine pigment concentrations in salmon fillets, with prediction errors of only 0.4 mg kg− 1 astaxanthin, and a correlation with chemically determined pigment of r = 0.92 (p < 0.0001). The results obtained in the present study are the first to demonstrate successful non-invasive pigment predictions in whole salmon using VIS/NIR spectroscopy, and the possibility for simultaneous, rapid and non-destructive quantification of fat and pigment concentrations.  相似文献   

10.
Low feed consumption has been suggested as a major impediment for the development of effective formulated diets for spiny lobsters. To seek an explanation for the low feed consumption, this study compared the feed consumption, appetite revival, foregut evacuation and fecal production of different sizes of spiny lobster Jasus edwardsii juveniles fed a fresh natural diet (i.e. mussel flesh) and a dry formulated diet. In addition, the effect of feeding frequency was determined by feeding juvenile lobsters the same ration of formulated diet at different time intervals. i.e., same ration delivered 3 week− 1, 1 day− 1, 2 day− 1, 5 day− 1 and 5 night− 1. Lobster (10–15 g) growth and feed consumption (1.15% BW day− 1) was maximized when receiving a single ration daily of the formulated diet. A slow appetite revival (AR > 18 h) on the formulated diet was consistent with the negative effect of increasing feeding frequency on growth and consumption. In addition, there appeared to be no major advantage in dry matter intake by providing the nutrient dense dry formulated diet to Jasus edwardsii. Lobsters fed fresh mussel flesh (79.8% moisture) consumed similar levels of dry matter as those lobsters fed the dry formulated feed (7.3% moisture). This appears to be due to high moisture gain and expansion of the dry feed in the lobster foregut. Foregut evacuation lasted 10 h for the two diets but a delay in initial foregut evacuation and processing of the formulated diet was evident, and may have negatively affected feed consumption, appetite revival and dry matter digestibility (61.2%) of the dry formulated diet compared to the mussel flesh diet (89.2%). Appetite revival on mussel flesh was almost complete by 12 h after a satiation ration and was governed primarily by the rate of foregut evacuation. Differences in the timing of foregut evacuation and fecal production between the two diets suggested that a greater proportion of the formulated diet entered the midgut directly.The results indicate that J. edwardsii cannot achieve high levels of feed consumption on formulated diets because gut throughput is reduced due to a small foregut capacity, expansion of the feed post-ingestion, lengthy foregut filling time (1–2 h) and clearance time (10 h), combined with slow gut throughput time (34–42 h) and appetite revival (> 18 h). A slow appetite revival and difficulties in processing and digestion of formulated diets appear as major issues to be resolved to improve the performance of formulated diets required for the commercial aquaculture of J. edwardsii and possibly other spiny lobsters.  相似文献   

11.
Immunolocalization by using rabbit polyclonal antibody against APGWamide demonstrated that there was APGWamide immunoreactivity (APGW-ir) in the varicosed nerve fibers in the capsule, connective tissue and trabeculae of the testis whereas there was no APGW-ir in the ovary of sexually mature broodstocks of Haliotis asinina. In vivo bioassay, by injecting APGWamide into foot muscle of mature males and females (age over 24 months), showed that most males (87.50%) injected with 200 μl of 10− 3 M APGWamide (170 μg APGWamide/animal) could be induced to spermiate whereas fewer males (43.75%) injected with 200 μl of 10− 6 M APGWamide (0.17 μg APGWamide/animal) could spermiate. Following injection with 10− 3 M, 53% of males spawned within 3 to 4 h, while the remaining males spawned within 2 to 3 h. The former tended to release more sperm at the range of 1.04 to 2 × 109, and the latter at the range 3.62 × 107 to 2.07 × 108 cells, respectively, and that the sperm of the former group appeared to be more motile. Following the injection of 10− 6 M, all inducible males spawned within 2–3 h, and 43% of spawned males have sperm around 2 × 109, while 57% have fewer sperm number ranging from 2.75 × 107 to 9.94 × 108 cells. In contrast to males, none of the mature females with ripen ovary could be induced to ovulate. These data suggest that APGWamide is an important signaling molecule that plays a role in the male reproductive process that could be used to induce spermiation.  相似文献   

12.
The effects of feeding frequency (1, 2, 3, or 15 times daily) on oxygen consumption (OC, mg O2 kg−1 h−1) and ammonia excretion, (AE, mg TAN kg−1 h−1) of walleye (Stizostedion vitreum) are described. Walleye were reared at a practical culture density of 35·4 kg m−3 in a single-pass system at 23·2°C. Diurnal variation in metabolic rates were related to feeding, not to photoperiod. Minimum OC rates occurred 30 min before the first feeding of the day, which was the longest average time since the last feeding. Metabolic rates increased immediately after feeding. The maximum rates for OC were 36–49% higher than the minimum rates, and 14–22% higher than the 24-h mean rate. Maximum rates for AE were 137–409% higher than the minimum rates, and 39–87% higher than the mean rates. There was a highly significant difference in the mean metabolic rates related to feeding frequency. The mean OC rate for 1 feeding day−1 (222·0 mg O2 kg−1 h−1) was greater than the mean rates for 2, 3 and 15 feedings day−1. The OC rate for 1 feeding day−1 was 50·6% greater than the rate for 15 feedings day−1 (147·4 mg O2 kg−1 h−1), the lowest mean rate. Mean and maximum oxygen/feed ratio (OFR, kg O2 kg−1 feed fed day−1), varied inversely with feeding frequency. The mean ammonia/feed ratio (AFR) was similar for all but the 3 feedings day−1 treatment, but the maximum AFRs for 2 and 15 feedings day−1 were lower than the AFRs for 1 and 3 feedings day−1. AE was directly proportional to OC; the regression equations were highly significant, but specific for feeding frequency.  相似文献   

13.
An 8-week feeding trial was conducted in a flow-through system (1–1.5 L min−1) at 27°C to determine dietary protein requirement for Channa punctatus fingerlings (4.58 ± 0.29 g) by feeding six isocaloric diets (18.39 kJ g−1, gross energy). Diets containing graded levels of protein (300, 350, 400, 450, 500 and 550 g kg−1) were fed to triplicate groups of fish to apparent satiation at 09:00 and 16:00 h. Maximum absolute weight gain (AWG; 8.11 g fish−1), specific growth rate (SGR; 1.82%) and best feed conversion ratio (FCR; 1.48) were recorded in fish fed diet containing 450 g kg−1 protein, whereas protein efficiency ratio (PER; 1.52), protein retention efficiency (PRE; 25%), energy retention efficiency (ERE; 78%) and RNA/DNA ratio (3.01) were maximum for the group fed dietary protein at 400 g kg−1. Second-degree polynomial regression analysis of AWG, SGR and FCR data against varying levels of dietary protein yielded optimum dietary protein requirement of fingerling between 462.24 and 476.72 g kg−1, whereas the regression analysis of PER, PRE, ERE and RNA/DNA ratio data showed a lower protein requirement of 438.28–444.43 g kg−1 of the diet. Considering the PER, PRE, ERE and RNA/DNA ratio as more reliable indicators, this protein requirement is recommended for developing quality protein commercial feeds for C. punctatus fingerlings.  相似文献   

14.
The present research investigated the effect of carbon/nitrogen ratio (C/N ratio) control in ponds with or without substrate addition for periphyton development on production of giant freshwater prawn. C/N ratios of 10, 15 and 20 were investigated in 40 m− 2 ponds stocked with 2 prawn juveniles (5.023 ± 0.02 g) m− 2 with or without added substrates for periphyton development. The various treatment combinations of C/N ratio and periphyton substrate addition are abbreviated as ‘CN10’, ‘CN15’, ‘CN20’, ‘CN10 + P’, ‘CN15 + P’ and ‘CN20 + P’, P representing periphyton substrate. A locally formulated and prepared feed containing 30% crude protein with C/N ratio10 was applied. Tapioca starch was used as carbohydrate source for manipulating C/N ratio and applied to the water column separately from the feed. Increasing the C/N ratio from 10 to 20 reduced (P < 0.001) the total ammonia-nitrogen (TAN), nitrite–nitrogen (NO2–N) and nitrate–nitrogen (NO3–N) in water column and total Kjeldahl nitrogen (TKN) in sediment. The addition of substrates only influenced the NO2–N concentration in the water column (P < 0.001). Increasing the C/N ratio raised the total heterotrophic bacterial (THB) population in the water column, sediment and periphyton (P < 0.001). It also increased the dry matter (DM), ash free dry matter (AFDM), and chlorophyll a content of periphyton (P < 0.001). The lowest specific growth rate (SGR), the highest food conversion ratio (FCR), and the lowest protein efficiency ratio (PER) were recorded in treatment CN10 (P < 0.05). The addition of substrates did not influence size at harvest (P > 0.05) but improved the survival from 62.8 to 72% (P < 0.001). Increasing the C/N ratio from 10 to 20 increased the net yield by 40% and addition of substrate increased the net yield by 23%. The combination of C/N ratio control and substrate addition increased the net yield by 75% from 309 (CN10) to 540 (CN20 + P) kg ha− 1 (120 days)− 1. This 75% higher production concurred with (1) a lower inorganic nitrogen content in the water column, (2) a higher THB abundance supplying additional single cell protein to augment the prawn production, and (3) an improved periphyton productivity and quality.  相似文献   

15.
The growth performance and stress response in striped knifejaw, Oplegnathus fasciatus (body weight 100–300 g) reared under four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) were investigated. Fish were fed a commercial diet to apparent satiation, two times a day for 8 weeks. A trial of acute handling and confinement stress was also carried out to investigate the stress-induced levels of different stress indicators in O. fasciatus. Blood was also collected from undisturbed fish which was considered as control. Although there were no significant differences in weight gain, specific growth rate (SGR) and feed conversion efficiency (FCE) in fish exposed to 6L:6D, 16L:8D and 24L:0D photoperiods, all parameters in these photoperiods were significantly higher than those of 12L:12D photoperiod (P < 0.05). There was no significant difference in protein retention efficiency (PRE) between fish exposed to 16L:8D and 24L:0D photoperiods, but PRE in both photoperiods was significantly higher than that of 12L:12D photoperiod.Acute stress significantly increased the plasma levels of cortisol (110.3 ng ml− 1) and glucose (195.4 mg dl− 1), and decreased plasma levels of total protein (0.8 g dl− 1); however, all parameters were returned back to the levels indistinguishable from those of control, undisturbed fish within 24 h. The levels of cortisol, glucose and total protein in fish exposed to different photoperiods during the study were far from the stress-induced levels (P < 0.05). The results demonstrated that the growth performance of O. fasciatus reared from 100 to 300 g can be stimulated significantly by using the manipulated photoperiods where feeding time may be playing an important role to increase food intake and feed conversion efficiency. It also revealed that the artificial photoperiods did not cause significant stress response in fish.  相似文献   

16.
In this study, the pharmacokinetic profile of enrofloxacin (EF) and its major metabolite, ciprofloxacin (CF), were investigated in brown trout (Salmo trutta fario) (n = 150) after intravenous (i.v.) and oral (p.o.) administrations of a single dose of 10 mg kg− 1 body weight (b.w.) at 10 °C. The plasma concentrations of the drugs were determined by high-performance liquid chromatography (HPLC-UV) from 0.08 to 120 h. Pharmacokinetic parameters were described by the two-compartment open model for intravenous and oral administrations, respectively. After intravenous administration, the elimination half-life (t1/2β), apparent volume of distribution at steady-state (Vss) and total body clearance (Cltot) of enrofloxacin were 19.14 ± 1.51 h, 3.40 ± 0.18 L kg− 1 and 0.14 ± 0.01 L kg h− 1, respectively. After oral administration, the maximum plasma concentration (Cmax), time of maximum concentration (tmax) and bioavailability (F%) were 2.30 ± 0.08 µg mL− 1, 8 h and 78 ± 4%, respectively. Ciprofloxacin was not detected in the present study. The elimination half-life for enrofloxacin following oral administration was longer than values calculated for other animals. After oral administration, the mean plasma concentration was well above the minimum inhibitory concentrations (MICs)—that is, > 0.5 µg mL− 1 at 36 h—for most gram-negative fish pathogens. It is possible and practical to obtain therapeutic blood concentrations of enrofloxacin in brown trout (S. trutta fario) using oral administration of 10 mg kg− 1 body weight; therefore, it may be effective in the therapy for brown trout diseases.  相似文献   

17.
The effects of dietary protein and energy level on spawning performance of Nile tilapia broodstock and hatchability of their eggs were studied. Nine diets containing three protein (30, 35 and 40%) and three energy (14.6, 16.7 and 18.8 MJ GE/kg) levels were prepared. The diets were fed to duplicate groups of broodfish (average weight of 60.5 ± 4.6 g for males and 39.4 ± 3.1 g for females) at 2–3% of their body weight, twice a day, for 150 days. At low protein level (30%), time to first spawning was significantly longer than at 35 and 40% protein levels, and increased with increasing dietary energy (decreasing protein-to-energy (P/E) ratio). At 35 and 40% protein levels, time to first spawning was not affected by dietary energy. Inter-spawning intervals (ISI) showed irregular patterns in relation to dietary protein and energy, however, at 40% protein the females tended to spawn at shorter intervals, regardless of dietary energy. Spawning performances, including total number of spawnings per tank, number of spawnings per female, absolute fecundity and average number of eggs per spawn, were all significantly lower (P < 0.05) at 30% CP than at higher protein levels. At all protein levels, increasing dietary energy from 14.6 to 18.8 MJ GE/kg (and decreasing P:E ratio) resulted in a significant decrease in fish fecundity. Egg size was not significantly affected by dietary protein and energy. At all energy levels, egg hatchability increased with increasing dietary protein levels (P < 0.05). Eggs produced from broodstock fed 30 and 35% CP, exhibited significantly lower hatchability (P < 0.05) and needed less time for hatching and yolk-sac absorption, and resulted in lower larval length than those fed a 40% protein diet. However, increasing energy level at each dietary protein level did not significantly affect these parameters. These results revealed that the best spawning performance of Nile tilapia broodstock reared in a recycling system was achieved at 40% dietary protein and 16.7 MJ GE/kg, with a P/E ratio of 23.6 g/MJ.  相似文献   

18.
The growth rate of disk abalone, Haliotis discus hannai, energy consumption and changes in water quality were monitored in a pilot-scale recirculating aquaculture system (RAS) for 155 days. Baffles were installed in the RAS culture tanks to enlarge the attachment area and clean out solid waste materials automatically by hydraulic force only. The experimental disk abalones, of shell length 24.5 ± 0.5 mm, were cultured at three stocking densities, 700, 1300 and 1910 individuals/m2 bottom area, in triplicate. The abalones were fed with sea mustard, Undaria pinnatifida, once a week. The abalone feed conversion rates and daily growth rates ranged from 24.5 to 25.9 and 0.32 to 0.36%, respectively. Their daily shell increments and survival rates ranged from 67.7 to 78.6 μm/day and 87.6–92.2%, respectively. The growth in weight tended to decrease at a culture density of 1300 individuals/m2 bottom area, while shell increments and survival rates were acceptable at this density. The total power consumption for heating was 1185.4 kW, comprising 30.2% of the total power consumption, while the average water exchange rate was only 2.9% per day. The total ammonia nitrogen stabilized below 0.07 mg/L, after conditioning of the biofilter. The NO2−–N, NO3−–N and total suspended solid concentrations were also maintained within acceptable ranges for the normal growth of disk abalone. The use of the RAS with these newly designed culture tanks for disk abalone culture produced 1300 individuals/m2 bottom area with a water exchange rate of only 2.9% per day and used about one-tenth of the heat energy of a conventional flow-through system.  相似文献   

19.
Formulated abalone feeds used by the culture industry are believed to be unsuitable for use at elevated water temperatures (>20 °C). The aim of this study was to develop a feed that could safely be fed to abalone cultured at elevated water temperatures by optimizing dietary protein/energy levels. Abalone (54.90 ± 0.08 mm; 28.99 ± 0.16 g) were cultured at either 18, 22 or 24 °C, and fed diets containing graded levels of protein (18–26%) and energy (11.6–16.2 MJ kg?1). Abalone growth was temperature dependent, declining from 4.33 g month?1 at 18 °C to 0.77 g month?1 at 24 °C. Shell length and weight gain were independent of dietary protein, provided that the digestible energy content of the diet was not lower than 13.5 MJ kg?1. Dietary energy levels below 13.5MJ kg‐1 resulted in significant reductions in shell growth, weight gain and increased mortality from 5% to 27%. Feed consumption of the 22% and 26% protein diets with 11.6 MJ kg?1 was significantly higher (0.53 ± 0.04 and 0.55 ± 0.04% bd. wt. day?1 respectively) compared with abalone fed the 16.2 MJ kg?1 diets at the same protein levels (combined mean of 0.45 ± 0.04% bd. wt. day?1) indicating that consumption was linked to energy requirement. The growth and mortality results suggest that abalone cultured at these temperatures have a dietary digestible energy requirement of at least 13.5 MJ kg?1.  相似文献   

20.
Mesocosms containing lateritic soil from West Bengal, India and de-ionized water were treated with different amounts of nitrogen (N), phosphorus (P), and potassium (K) fertilizers. Concentrations of available N, P, and K in soils and gross primary productivity were measured for the mesocosms. Critical concentrations of the three nutrients were estimated by the graphical method of Cate and Nelson that has been used widely for establishing critical soil nutrient concentrations in fertilization research on terrestrial crop plants. Critical concentrations of the three nutrients were: available N, 200 mg kg−1; available P, 13 mg kg−1; available K, 80 mg kg−1. Ponds sited in red, lateritic soil in West Bengal were treated monthly with 20, 15, and 3 kg ha−1 application−1 of N, P, and K, respectively. There was an increase in gross primary productivity when the critical concentrations of available N, P, and K established in the mesocosm study were attained in the pond soils. Fish production in fertilized ponds is positively correlated with gross primary productivity. Thus, the concept of critical nutrient concentrations in soils could be a valuable tool in pond fertilization research.  相似文献   

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