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为探索禽脑脊髓炎病毒强毒VR株对SPF鸡的致病性,开展了不同接种途径和不同日龄SPF鸡的致病力试验。不同接种途径致病力试验结果表明,AEV VR株口服后不能引起发病;刺种、肌肉注射途径接种随着日龄增大,发病率降低;脑内注射途径接种可引起试验鸡全部发病。最小致病量试验结果显示,VR株脑内注射攻毒的最小致病量为10~(2.0) EID_(50)。不同日龄SPF鸡的致病力试验结果表明,随着试验鸡日龄的增大,其致病力略有差异,日龄越小,发病率越高。结果表明,AEV VR株脑内攻毒70日龄内SPF鸡的发病率为80%及以上,攻毒剂量为10~(4.0)EID_(50)。 相似文献
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为比较血液和粪便等样品采用不同方法检测禽网状内皮组织增殖病毒（REV）的准确率,从而为制定禽网状内皮组织增殖病（RE）净化程序提供参考,利用鸡胚卵黄囊接种构建REV垂直传播感染雏鸡模型,分别采用DF-1细胞病毒分离、RT-PCR检测和荧光定量RT-PCR检测,对1日龄雏鸡血液和胎粪样品,7～70日龄鸡（每隔1周）的血液和泄殖腔棉拭子样品,进行REV检测和结果比较。结果显示：对1日龄出壳的SPF鸡血浆进行检测,荧光定量RT-PCR灵敏度最高,阳性检出率为100%(25/25),其次为病毒分离,阳性检出率为96%(24/25),RT-PCR检出率最低;对1日龄出壳的SPF鸡胎粪进行检测,荧光定量RT-PCR与病毒分离灵敏度一致,阳性检出率均为80%(20/25),RT-PCR检出率最低,为28%(7/25)。对7～70日龄鸡（每隔1周）进行血液和泄殖腔棉拭子检测,3种方法阳性检出率与1日龄检测情况总体相似,检出率从高到低依次为荧光定量RT-PCR、病毒分离和RT-PCR。结果表明,在同一检测时间点使用同一检测方法,胎粪/泄殖腔棉拭子REV阳性检出率基本低于血液检出率。本研究初步比较了不同方... 相似文献
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广西鸡传染性贫血流行病学的研究 总被引：10，自引：0，他引：10
应用PCR检测技术 ,对采自广西五个主要养鸡地区 5 0多个鸡群 3 2 1羽病鸡的组织样品 ,进行了鸡传染性贫血病毒核酸的检测。结果检测的病例平均阳性率为 2 8 3 4%;三黄鸡的阳性检出率最高 ( 3 9 2 8%) ;各种日龄的鸡均可检出 ,其中最小的为 7日龄、最大的为 3 5 0日龄 ;对 5个免疫器官的检测结果显示 ,骨髓的检出率最高( 84 3 8%) ;CIAV阳性病例中与MDV、REV和ALV的混合感染率共计 48 40 %。 相似文献
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鸽新城疫的诊断及病原分离鉴定 总被引：1，自引：1，他引：0
20 0 1年 1 0月中旬 ,江苏某养鸽专业户饲养的 30 0多对美国白羽王肉用鸽 ,部分鸽出现以神经症状和腹泻为主要特征的疫病 ,发病率为 30 %左右 ,死亡率为 2 0 %左右。经临床检查 ,病理剖检 ,初步诊断为鸽新城疫 (ND)。立即对全群鸽用新城疫疫苗紧急接种 ,迅速扑灭了疫情 ,同时从病死鸽体内分离到1株新城疫病毒 (NDV)。1 材料与方法病料 :无菌采集病死鸽脑、肝、肺及提取的气管拭子。血清 :发病鸽血清共 30份 ;ND、EDS阳性血清 ,自制 ;SPF鸡血清 ,由江苏省家禽研究所提供。鸡胚 :1 0日龄非免疫鸡胚 ,从江苏省家禽研究所购进种蛋 ,37℃… 相似文献
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为了查明安徽滁州温氏公司矮脚黄D品种发生疑似传染性法氏囊病的病因,临床调查后采集相应病料,实验室研磨处理后备用。首先对病料进行鸡传染性贫血病毒(CAV)和传染性法氏囊病病毒(IBDV)的PCR检测。第1次病例复制,病料攻毒SPF鸡观察病变,并采集病料和血清进行相关抗原和抗体的检测;第2次病例复制,采集SPF鸡病料进行氯仿和热处理,使病料中仅保留CAV后再攻毒SPF鸡观察病变,并采集病料和血清进行相关抗原和抗体的检测。结果:4份病料有3份CAV PCR阳性,1份IBDV F1代尿囊液PCR阳性。第1次病例复制出现和临床相似的IBDV典型病变,第2次病例复制出现CAV典型病变。因此表明本次疫情为CAV与IBDV混合感染引起。 相似文献
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禽脑脊髓炎 (AE)由禽脑脊髓炎病毒 (AEV)引起 ,主要侵害幼龄鸡。其特征是患病鸡运动失调、头颈震颤 ,一旦发病 ,无特效治疗药物 ,只能采取综合性防制措施。1998年 10月份 ,笔者发现了两批发生脑脊髓炎的雏鸡 ,经过采取一系列措施 ,疾病得到了控制。1 发病情况与临床症状某父母代鸡场于 1998年 10月 16日、10月 2 5日孵化的两批雏鸡 (第 1批 5 50 0只 ,第 2批 4 30 0只 )分别于 5日龄 ,7日龄发现少数病鸡 ,表现为瘫痪 ,不爱走动 ,强迫运动时 ,步态蹒跚 ,前后摇晃或摔倒 ,饮水、食欲基本正常 ,粪便变化不大。病初怀疑鸡腿脚受损伤所致 ,但 … 相似文献
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Detection of avian encephalomyelitis virus 总被引：2，自引：0，他引：2
Methods for the detection of two strains of avian encephalomyelitis virus (AEV) in chick embryo brain cell cultures and chickens were compared. It was found that the agar gel precipitin test (AGPT) and the enzyme-linked immunosorbent assay (ELISA) carried out on the serum of inoculated chickens were more sensitive than either the indirect fluorescent antibody test in cell cultures or the detection of clinical signs in chicks. On the basis of results obtained in this experiment the effects were then determined of routes and time of inoculation of chickens on the detection of AEV. It was found that birds infected at two weeks old produced higher antibody titres than one-day-old birds and the AGPT and ELISA detected comparable levels of antibody in them. It was recommended that the tests to detect the presence of AEV as a contaminant of vaccines be replaced by a serological test carried out on chicks inoculated intramuscularly at two weeks old. 相似文献
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An agar-gel-precipitin (AGP) antigen for avian encephalomyelitis virus (AEV) was prepared from infected chicken embryo brains. The antigen could precipitate specific antibodies to AEV. No nonspecific reactions were observed. Results of AGP tests were compared with those of virus-neutralization (VN) tests on both unvaccinated and AEV-vaccinated chickens. The AGP test reliably detected antibodies to AEV as early as four days postinoculation. Antibodies persisted in most vaccinated birds for over one year. 相似文献
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The dynamics of the production of immunoprecipitation antibodies to Marek's disease virus was studied in the serum of chickens with maternal antibodies in relation to the occurrence of the immunoprecipitation antigens of Marek's disease virus in feather follicles. One-day-old chickens were infected by the contact method with Marek's disease virus. The first occurrence of immunoprecipitation antigen was detected on the 14th day after infection and this occurrence persisted throughout the experiment, i. e. until the 112th day after infection. The antibodies were first detected the 28th day after infection and their titre kept rising until the 98th day after infection. Immunoprecipitation antibodies and antigens of Marek's disease virus were detected in some tumorously changed kidneys. Immunoelectrophoretic examination revealed in the same kidneys immunoglobulins of the class IgY, IgA and beta-globulin. The slowest-migrating fraction of IgY, together with IgA, beta-globulin and C-reactive protein were detected in the skin extracts from infected poultry. Indirect haemagglutination enabled the detection of the presence of haemagglutination antibodies in rabbit immunoglobulin to the skin antigen of Marek's disease virus, and in avian immunoglobulin to the same virus. Haemagglutination antigen was revealed in the extract from tumorously changed kidneys. 相似文献
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本研究的病毒分离物,是来源于天津地区某鸡场暴发的28日龄病鸡。此毒株对SPF雏鸡几乎100%感染发病,死亡率达30%～90%,此毒有很强的亲肾组织性,引起肾病变肿大、苍白、斑驳状,肾小管和输尿管有尿酸盐沉积。对SPF鸡胚感染率98%～100%,可致鸡胚侏儒化、卷曲等特征性病变。在10日龄SPF鸡胚连传10代后,其毒价可达106.5EID50/0.1ml。鸡胚毒不凝集鸡红细胞,可干扰NDV血凝素的生成,而其本身又可被AEV所干扰,能加速气管纤毛止动效应,电镜观察呈圆形或多样性,边缘不整,表面有纤突的病毒粒子,直径约100mm。病毒中和试验进行血清学鉴定,此毒对Gray株、Holte株和澳大利亚T株的中和保护率均低于50%,而对M41株则高于50%,此毒株具备冠状病毒的主要特征,定名为“鸡传染性支气管炎嗜肾性病毒X株”(NephropathogenicInfectiousBronchitisVirusStrainX)——NIBVX。 相似文献
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为进一步研究采用卵黄抗体检测代替血清抗体检测的可行性,采用已建立的卵黄抗体ELISA检测方法,比较同一时期卵黄抗体与血清抗体的相关性。人工感染23周龄无特定病原体（SPF）鸡,每周采集全血分离血清,每天收集种蛋,ELISA方法检测血清、种蛋中的REV抗体,并进行比较,结果表明：攻毒后第2周开始血清抗体和卵黄抗体呈阳性,并达到峰值,之后抗体水平缓慢下降,两者具有相似的消长规律;对同一时期的卵黄抗体和血清抗体S/P比值进行统计学比较,P值小于0.05,相关系数为0.931,表明两者差异性不显著,相关性很好;阴阳性符合率比较,阳性符合率为97.8%,阴性符合率为100%,总体符合率为98.4%,阴阳性复合率很高。试验证明,同一时期的卵黄抗体和血清抗体的相关性很好,可用卵黄抗体检测方法代替血清抗体检测,从而为监测SPF鸡群感染REV提供新的技术手段。 相似文献
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Development and application of an ELISA technique for the detection of antibody to avian encephalomyelitis viruses 总被引：1，自引：0，他引：1
A rapid sensitive enzyme-linked immunosorbent assay for the detection of antibody to avian encephalomyelitis viruses (AEVs) in chickens using purified antigen is described. The procedure differed from others which have been described for AEV, in that it involved a negative antigen subtraction step which accounted for the variable adhesiveness of chicken sera to plastic surfaces. The procedure was reproducible (between-assay coefficient of variation 8.95 per cent) and a good correlation was observed with results obtained by neutralisation index tests (r = 0.91, P less than 0.1). The assay detects only AEV-specific antibody and allows monitoring of the spread of AEV in flocks. 相似文献
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100只SPF鸡均分为5组，A、B、c3组免疫后攻毒，接种3批次自制的IBD基因工程重组亚单位油乳剂疫苗；D组不免疫不攻毒，E组不免疫而攻毒。免疫后第22天，感染IBDV强毒株BC-6／85。攻毒后第4天，将所有存活的鸡只以颈脱臼致死，收集法氏囊，以3种方法和指标（法氏囊眼观病变；法氏囊显微病变；法氏囊中IBDV抗原检测）进行分析，以评定免疫保护率。结果显示，以这3种方法和指标评定，A组免疫保护率为90％，B、C组免疫保护率均为95％；试验鸡A3、A14、B7、C16、E1～E20，法氏囊眼观病变明显，法氏囊显微病理损伤评分为3～5分，琼脂免疫扩散试验检测法氏囊中IBDV抗原均为阳性；其他试验鸡，法氏囊眼观无明显异常，法氏囊显微病理损伤评分在3分以下，琼脂免疫扩散试验检测法氏囊中IBDV抗原均为阴性。结果表明，这3种方法和指标在IBD疫苗免疫保护试验评定中具有很好的一致性。 相似文献
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O Onunkwo J O Okoye 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1991,44(4):411-414
Infectious bursal disease was reported in a flock of 7-week old vaccinated chickens. Clinical findings and post-mortem changes were classical as well as the microscopic pathology of the bursa. Bursal homogenates from dead birds were positive for IBD virus antigen in agar gel diffusion test (AGDT). Convalescent sera obtained from birds 14 days following the onset of clinical signs were also positive for IBD virus antibody in AGDT. Seven-week old susceptible birds, each infected i/m with 0.1 ml of a bursal preparation from the outbreak, showed clinical signs of IBD on the 3rd day and were all dead by the 6th day. Their bursae were also positive for IBD virus antigen in AGDT. This is the first recorded outbreak of IBD in Southern Nigeria following inoculation with a locally produced vaccine. 相似文献
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禽脑脊髓炎油佐剂灭活疫苗免疫效果观察 总被引：3，自引：1，他引：2
将感染了ＡＥＶ－ＶａｎＲｏｃｋｅｌ株的ＳＰＦ全胚磨制的乳剂离心分离,取上清液制成抗原含量不同的两种灭活疫苗。将两种疫苗各接种３月龄的ＳＰＦ鸡,未见接种鸡出现症状。４周后以强毒ＡＥＶ攻击,所有疫苗接种鸡皆被保护,组织学检查进一步证明了疫苗的效力。抗原含量较高的疫苗引起的保护力更好,作者认为攻击时经过脑内接种更适合于临床评价疫苗效力。 相似文献