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应用鸡胚培养、电镜观察、血清学试验及病理组织学检查，分离与鉴定了来自河南省鸡传染性法氏囊病（ＩＢＤ）免疫鸡群Ｈｅ１、Ｈｅ２、和Ｈｅ３三株ＩＢＤＶ毒株。鸡胚传代试验表明，Ｈｅ１和Ｈｅ２株连续传代到第４代出现规律性死亡和ＩＢＤ病变，而Ｈｅ３株在第１代时鸡胚即全部死亡，且出现ＩＢＤ典型鸡胚病变。Ｈｅ１、Ｈｅ２和Ｈｅ３毒株对易感鸡致病力有一定的差异。病死率分别为２０％、１０％和４０％，多数为弱毒株。免疫保护试验证明，用常规的ＩＢＤＶ血清Ｉ型病毒株Ｂ２疫苗对Ｈｅ１、Ｈｅ２和Ｈｅ３毒株的免疫保护试验，与对照组相比保护率分别为０％、０％和１０％。未死亡扑杀的免疫试验鸡法氏囊有不同程度的病理组织学病变，表明Ｉ型毒株疫苗对ＩＢＤ基本上无免疫保护作用。所分离的毒株可能是ＩＢＤＶ的变异株。 相似文献
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麻雀体内传染性法氏囊病病毒的分离鉴定及理化特性研究 总被引：5，自引：1，他引：4
从传染性法氏囊病（ＩＢＤ）阳性麻雀体内分离到了一株病毒，用传染性法氏囊病病毒（ＩＢＤＶ）单克隆抗体心ＥＬＩＳＡ试验和ＤＩＧ－标记ＩＢＤＶｃＤＮＡ探针班点杂交试验证明该病毒为ＩＢＤＶ。病毒可适应于鸡胚和鸡胚成纤维细胞，产生细胞病变效应（ＣＰＥ）。病毒血清型为Ｉ型，病毒代谢抑制试验证明其基因组为ＲＮＡ，病毒核酸的电泳图谱呈两条特征带。病毒对乙醚不敏感ＰＨ２．０不能灭活可使病毒失感染性，５６℃作用３小时 相似文献
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鸡传染性法氏囊病野毒株的分离鉴定及河北弱毒株培育的研究 总被引：4，自引：3，他引：1
从河北省６个地区鸡传染性法氏囊病（ＩＢＤ）的鸡群中，分离到６株强毒，均能使易感鸡３６小时发病并死亡，发病率达６０～１００％，死亡率１０～７０％。人工感染鸡能见到与野外病例相同的病变。经血清学试验、鸡胚接种、电镜观察均证明，分离物为传染性法氏囊病毒（ＩＢＤＶ），其中有的毒株，毒力非常强。利用其中毒力最强的１株病毒，经鸡胚多次传代培养育成１株免疫原性好，免疫期长，免疫效果容易监测的弱毒株，经野外试验证明，该毒株是防治ＩＢＤ的一个很理想的毒株。 相似文献
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鸡,鸭体内传染性法氏囊病病毒的分离及理化性质比较 总被引：2，自引：0，他引：2
从疑似传染性法氏囊病（ＩＢＤ）病鸡及同群饲养的鸭体内各分离到１株病毒，用传染性法氏囊病病毒（ＩＢＤＶ）单克隆抗体夹心ＥＬＩＳＡ试验证明两病毒均为ＩＢＤＶ，病毒血清型为Ⅰ型。病毒可致死鸡胚，适应于鸡胚成纤维细胞并产生细胞病变（ＣＰＥ）。理化性质比较表明，两病毒为同源ＩＢＤＶ。研究表明，鸭可成为ＩＢＤＶ的携带者或传染源。 相似文献
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将临床分离的疑似鸡传染性法氏囊病病毒（ＩＢＤＶ）山东分离株，人工接种４０～５０日龄ＳＰＦ鸡，取死亡鸡法氏囊和脾脏，用匀浆器制备组织悬液，并经低温反复冻融４次，离心取上清液，ＰＥＧ沉淀，再经蔗糖梯密度离心进一步纯化。经电镜观察、吸光度测定和琼脂扩散试验，结果表明，分离物为ＩＢＤＶ。 相似文献
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鸡传染性法氏囊病毒强毒株的分离鉴定 总被引：2，自引：0，他引：2
从广州市郊区某鸡群送检的６周龄曾经ＩＢＤＶ免疫的病鸡法氏囊中分离到１株ＩＢＤＶ强毒。该毒株能使鸡胚于接种后３６～７２小时内死亡，易感鸡１００％发病，５０％死亡。电镜观察，该病毒颗粒直径６０ｎｍ左右，无囊膜。经ＳＤＳ－ＰＡＧＥ检测，病毒核酸有二条ＲＮＡ带。 相似文献
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鸡传染性法氏囊病病毒的提纯和鉴定 总被引：5，自引：0，他引：5
将临床分离的疑似鸡传染性法氏囊病毒（ＩＢＤＶ）山东分离株，人工接种４０～５０日龄ＳＰＦ鸡，取死亡鸡法氏囊和脾脏，用匀浆器制备组织悬液，并经低温反复并融４次，离心取上清液，ＰＥＧ沉淀，再经蔗糖梯度离心进一步纯化，经电镜观察，吸光度测定和琼脂扩散试验，结果表明：分离物为ＩＢＤＶ。 相似文献
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鸡传染性法氏囊病病毒野毒株VP2基因高可变区酶切位点分析 总被引：5，自引：1，他引：4
参照澳大利亚鸡传染性法氏囊病病毒（ Ｉ Ｂ Ｄ Ｖ）００２７３ 毒株基因组序列设计的 １ 对引物,位于 Ｖ Ｐ２ 高可变区两端,通过 Ｒ Ｔ Ｐ Ｃ Ｒ,扩增了 ５ 个 Ｉ Ｂ Ｄ Ｖ 山东分离株 Ｖ Ｐ２ 基因的 ６０７～１ ０８０ 位核苷酸片段（ａａ２０３～３０６）。 Ｐ Ｃ Ｒ 产物经纯化后,分别用 Ｄｒａ Ⅰ、 Ｓａｃ Ⅰ、 Ｓｓｐ Ⅰ、 Ｐｓｔ Ⅰ和 Ｓａｕ３ Ａ Ｉ共 ５ 种限制性内切酶（ Ｒ Ｅ）进行消化处理,结果 ５ 个分离株均为 Ｄｒａ Ⅰ（－ ）、 Ｓａｃ Ⅰ（－ ）和 Ｓａｕ ３ Ａ Ｉ（＋ ）, Ｌ Ｃ２ 和 Ｔ Ａ 株为 Ｓｓｐ Ⅰ（＋ ）, Ｌ Ｃ１ 和 Ｊ Ｎ 株为 Ｓｓｐ Ⅰ（－ ）, Ｌ Ｃ１ 和 Ｌ Ｃ２ 株为 Ｐｓｔ Ⅰ（＋ ）, Ｊ Ｎ、 Ｌ Ｌ 和 Ｔ Ａ 株为 Ｐｓｔ Ⅰ（－ ）;５ 个分离株的酶切图谱与美国变异株迥异,而 Ｔ Ａ 株与日本超强毒株 ９０１１ 相类似。５ 个分离株与现用疫苗株对比,至少在 Ｖ Ｐ２ 可变区内存在着一定的差异,这可能是 Ｉ Ｂ Ｄ Ｖ 接种免疫鸡群仍然暴发 Ｉ Ｂ Ｄ 的原因之一。 相似文献
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PCR检测鸡减蛋综合征病毒 总被引：3，自引：0，他引：3
根据已报道的鸡减蛋综合征（ＥＤＳ－７６）病毒ＤＮＡ序列，设计合成了１对引物，建立了ＥＤＳ－７６病毒的双温式聚合酶链反应（ＰＣＲ）诊断方法。该方法对ＥＤＳ－７６病毒长春株、河南株和广东株扩增结果均为阳性；而对致死鸡胚孤儿病毒（ＣＥＬＯＶ）、鸡病毒性关节炎病毒（ＶＡＶ）、鸡传染性支气管炎病毒（ＩＢＶ）、鸡传染性法氏囊病病毒（ＩＢＤＶ）和鸡新城疫病毒（ＮＤＶ）的扩增结果均为阴性。可检测ＥＤＳ－７６病毒ＤＮＡ为０．３×１０－４ｐｇ。结果表明该方法特异且敏感。此外，将常规的三温式ＰＣＲ的操作规程改为双温式，反应体积由常规５０～１００μＬ改为２０μＬ，使其更加快速、简便、经济 相似文献
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从疑似IBD病鸡的法氏囊组织中分离到1株ARV 总被引：5，自引：2，他引：3
从暴发类似传染性法氏囊病（ＩＢＤ）的江苏省某鸡场采集病鸡法氏囊组织制成组织悬液，接种鸡胚卵黄囊，部分鸡胚３～５ｄ死亡，部分鸡胚不死亡但有病变。用感染胚卵黄囊和绒尿膜混合物，接种鸡胚成纤维细胞（ＣＥＦ），盲传３代后，发现以合胞体为特征的细胞病变（ＣＰＥ）。感染细胞做超薄切片电镜观察，可见细胞浆内病毒粒子呈整齐的晶格状排列。用免疫沉淀法提取病毒抽提核酸，经ＳＤＳ－ＰＡＧＥ电泳，可见规律排列的１０条带，呈３－３－１－３排列。与禽呼肠孤病毒（ＡＲＶ）参考毒株Ｓ１１３３株的核酸谱带的数目和位置相同。血清学试验与ＡＲＶ呈阳性反应，与传染性法氏囊病病毒（ＩＢＤＶ）呈阴性反应。证明分离病毒为ＡＲＶ 相似文献
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从一个免疫失败鸡场中分离到一株鸡传染性法氏囊病病毒野毒株,命名为SD株.经血清学试验、鸡胚接种、病毒形态观察等证实了分离物为鸡传染性法氏囊病病毒(IBDV).测定病毒效价ELD50达到10-6.5/0.2 mL;动物回归试验表明,该SD株接种4周龄鸡后引起鸡群发病率为100%,病死率达85%,剖检可见法氏囊呈"紫葡萄样... 相似文献
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Okoye J.O.A. Aba-Adulugba E.P. Ezeokonkwo R.C. Udem S.C. Orajaka L.J.E. 《Tropical animal health and production》1999,31(2):75-81
One hundred 6-week-old susceptible cockerels were inoculated with a pathogenic strain of infectious bursal disease virus (IBDV) and kept in the same pen as 100 each of 6-week-old pullets, local chickens and broilers. The cockerels developed depression and diarrhoea on day 3 post inoculation (PI) and most of the pullets and some of the local chickens and broilers showed similar signs on day 4 PI. Loss in weight was severe and similar in the pullets and local chickens, being significantly greater than that in the broilers from days 3–11 PI. The total mortality was 85%, 66.7%, 30% and 20% for the pullets, cockerels, local chickens and broilers, respectively. The lesions were more severe in the pullets and local chickens than in the broilers. IBDV antigen and antibody were detected, respectively, in all the bursal and serum samples from the infected chickens tested. The contact exposure method used in this study simulates better what happens in nature than inoculation with IBDV. The reduced mortality observed among the local chickens, compared with that (61.5%) seen in earlier studies using intraocular inoculation of IBDV, may have been due to behavioural differences that tend to result in their ingesting a relatively low dose of the virus. 相似文献
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2011年8月,从河南省疑似传染性法氏囊病鸡群采集病料,通过鸡胚接种、琼脂扩散试验和RT-PCR等方法,证实分离的病毒为传染性法氏囊病病毒（Infectiousbursaldiseasevirus,IBDV）,命名为HB／11株。序列分析表明,HB／11株VP2基因的核苷酸序列与GenBank中发表的部分国内外IBDV超强毒株VP2基因序列相似性在99％左右;HB／11株VP2高变区基因含有七肽基序为SWSASGS,在222、253、256、279、284、294和299位上的氨基酸残基分别是A、Q、I、D、A、I和S,具有IBDV强毒的分子特征。动物回归试验表明,30日龄鸡群接种该毒株后引起鸡群发病率为100％,死亡率为70％,剖检可见鸡传染性法氏囊病典型病变。因此该病毒为IBDV强毒株。 相似文献
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鸡传染性法氏囊病超强毒感染后SPF鸡免疫器官病理学观察 总被引：8，自引：2，他引：6
IBDV超强毒株LX株接种2周龄SPF雏鸡后，其致病性不同于经典强毒株CJ801株，它主要引起接种鸡全身性炎症反应，法氏囊、脾脏、盲肠扁桃体等免疫器官中大量异嗜性白细胞、巨噬细胞浸润，淋巴细胞严重坏死崩解，胸腺皮质严重萎缩、坏死，骨髓中造血细胞减少、巨噬细胞和脂肪细胞增生。在接种后14d法氏囊淋巴滤泡严重萎缩、淋巴细胞排空形成囊腺样结构，未见恢复正常，其它免疫器官形态基本恢复正常。电镜观察，接种后2和4d可见胸腺淋巴细胞胞浆浓集、染色质周边化形成新月形，表现细胞凋亡特征；在法氏囊坏死淋巴细胞胞浆中可见60nm大小呈晶格排列或散在的病毒粒子。研究初步探明了鸡传染性法氏囊病病毒超强毒的致病机理。 相似文献
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Highly virulent infectious bursal disease virus (IBDV) was isolated from field cases, and the pathogenicity of the isolates was examined in specific-pathogen-free chickens. Chickens inoculated with the isolates developed severe clinical disease with a high mortality rate. Histopathologically, infectious bursal disease was characterized by bursal and thymic necrosis, aplastic anemia, acute hepatitis with fatty change, and systemic inflammatory response. In addition to functional abnormalities in the liver, a hypoxic state was induced by aplastic anemia and severe inflammation in the pulmonary air capillary walls. These pathological changes appeared to be closely related to the cause of death. 相似文献
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The characteristics of the pathogenic infectious bursal disease virus (IBDV) that infected avian species other than commercial chickens were largely unknown. In this study, by using in vivo and molecular methods, we had characterized an IBDV isolate (named 94268) isolated from an infectious bursal disease (IBD) outbreak in Malaysian village chickens--the adulterated descendant of the Southeast Asian jungle fowl (Gallus bankiva) that were commonly reared in the backyard. The 94268 isolate was grouped as the very virulent IBDV (vvIBDV) strain because it caused severe lesions and a high mortality rate in village chickens (>88%) and experimentally infected specific-pathogen-free chickens (>66%). In addition, it possessed all of the vvIBDV molecular markers in its VP2 gene. Phylogenetic analysis using distance, maximum parsimony, and maximum likelihood methods revealed that 94268 was monophyletic with other vvIBDV isolates and closely related to the Malaysian vvIBDV isolates. Given that the VP2 gene of 94268 isolate was almost identical and evolutionarily closely related to other field IBDV isolates that affected the commercial chickens, we therefore concluded that IBD infections had spread across the farm boundary. IBD infection in the village chicken may represent an important part of the IBD epidemiology because these birds could harbor the vvIBDV strain and should not be overlooked in the control and prevention of the disease. 相似文献
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ZHANG Zhen-hua LI Lin JING Xiao-dong ZHANG Jian-wei SHEN Jia SHI Ai-hua ZHENG Xiao-lan HUANG Feng-jun JIANG Bei-yu 《中国畜牧兽医》2015,42(9):2493-2498
Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%. 相似文献
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将鸡传染性法代囊病病毒(IBDV)BX株抗原和IBDV高免血清按一定比例混合,初步制备了无菌的鸡传染性法氏囊病免疫复合物疫苗1~5(分别含有32、8、4、0.5及0.125单位IBDV中和抗体),用鸡传染性法氏囊病免疫复合物疫苗1~5及BX株活疫苗分别免疫1日龄低母源抗体水平雏鸡,免疫后9 d观察法氏囊病变,免疫后28 d采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后9 d,复合物疫苗1~3免疫组鸡法氏囊正常,复合物疫苗4、5免疫组和BX株活疫苗免疫组分别有2/10、4/10和5/10的试验鸡法氏囊出现了病变;免疫后28 d,复合物疫苗1~5免疫组和BX株活疫苗免疫组IBDV中和抗体效价分别为8.34log2、9.60log2、9.21log2、7.88log2、9.50log2和9.12log2,攻毒保护率分别为90%、100%、100%、80%、90%和80%。试验结果表明鸡传染性法氏囊病免疫复合物疫苗2、3(分别含8、4单位IBDV中和抗体)免疫1日龄低母源抗体水平的雏鸡效果最好,攻毒保护率能达到100%。 相似文献