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1.
超声波处理对苹果花粉介导转基因的影响   总被引:3,自引:1,他引:2  
为了探讨花粉介导转基因过程中附加超声波处理对苹果品种丹霞和嘎拉花粉的影响,对苹果花粉处理的缓冲液和超声波的各参数进行了选择,设置了不同的缓冲液pH值、蔗糖浓度;超声波处理功率、时间、次数等。结果表明:适合苹果花粉处理的缓冲液蔗糖浓度为10%,pH值为5.4;超声波处理功率、时间、次数等各参数对苹果花粉的萌发率和破碎率有显著影响。  相似文献   

2.
摘要:通过秋季对无核白、克瑞森和红地球三个葡萄品种进行不同配方叶面肥喷施试验,第二年春季测量三个品种各处理葡萄芽的大小、芽的萌发率和花粉生活力,获得最佳秋季喷肥配方,以期为葡萄优质丰产栽培提供理论依据。结果表明,三个葡萄品种喷肥处理芽纵横径长度和花粉生活力均比对照好。处理4芽横径增幅最大,处理4和处理5花粉生活力高,与CK有显著性差异。三个品种各处理的纵径与花粉生活力有显著相关性。  相似文献   

3.
以天津汉沽茶淀玫瑰香葡萄为原料,采用低温结合超声波工艺对已经SO2终止发酵的玫瑰香白葡萄原酒进行进一步处理,通过研究不同处理温度(-2、2、6 ℃,室温对照)、不同低温处理时间(1、3、5、7 d)以及不同的超声波处理时间(40 kHz/10 min、40 kHz/20 min)对低醇玫瑰香白葡萄酒各项理化指标和感官指标的影响,确定最适低温结合超声波处理白葡萄酒的工艺条件。结果表明,在-2 ℃低温下处理葡萄原酒5 d,并结合40 kHz超声波处理葡萄原酒20 min,可有效地终止后期低醇甜白葡萄原酒的继续发酵。在此工艺条件下,可获得微生物稳定性高的具有高品质的低醇甜白葡萄酒。  相似文献   

4.
《华北农学报》2006,21(6):135-138
第1期phaG基因转化马铃薯的初步研究……………………………………………………………………………………孙环星,等(1)大麦品种资源筛选与TrxS基因的转化利用…………………………………………………………………………孔维威,等(5)葡萄花粉介导转基因超声波处理参数的选择……  相似文献   

5.
以离体的芝麻花粉为实验材料,研究了不同的培养基组分以及超声波处理对芝麻花粉萌发的影响.实验结果表明:蔗糖对于芝麻花粉的萌发有重要作用,当蔗糖浓度为35%时,芝麻花粉内外的渗透压达到平衡,有利于花粉萌发;培养基内添加适量的硼酸和氯化钙能有效地促进花粉萌发,但超过一定浓度时起抑制作用;培养基以35%的蔗糖,40 mg/L的硼酸,200 mg/L的氯化钙较为适宜,花粉的萌发率可以达到58%左右;超声波处理的功率、处理时间以及处理次数对芝麻花粉的萌发率和破碎率有显著影响.  相似文献   

6.
利用常压室温等离子体(ARTP)处理玉米种子和花粉,研究其对玉米种子萌发、幼苗生长和花粉萌发的影响。结果表明:ARTP处理5 min、15 min、30 min对玉米种子出芽率没有作用,但是影响了其幼苗的生长:随着处理时间的延长,幼苗干重逐渐增加;同对照相比,ARTP处理30 min幼苗显著增长,而幼苗根长/苗高比值降低。随着ARTP对玉米花粉处理时间的增加(0 s,18 s,54 s,90 s,144 s,180 s,270 s,360 s),花粉萌发率大幅度降低,处理360 s时,花粉萌发率接近为零。本研究将有利于ARTP在玉米诱变育种中的应用。  相似文献   

7.
超声波联合混凝法处理制药废水的研究   总被引:1,自引:1,他引:0  
为了探讨单独超声波降解法、混凝法及2种方法联合处理制药废水的可行性,研究了超声波辐射时间、功率以及混凝剂的种类、浓度对制药废水中COD和NH3-N去除的影响。结果表明:(1)超声波单独处理制药废水时,COD和NH3-N去除率先随着超声波辐射时间(200~1000 s)的增大而增大,最佳时间为1000 s,COD和NH3-N的最大去除率分别为27.80%、45.34%;超声波辐射时间为1000 s,COD和NH3-N去除率随着超声波辐射功率的增大而先增大后减小。(2)单独用混凝剂处理制药废水时,COD的去除率随着混凝剂的浓度(0.1~0.6 g/L)的增大而先增大后减小,PAC在0.3 g/L时为最佳,COD的去除率为40.10%,NH3-N的平均去除率保持在5%左右。(3)在各自的最佳状态下,先用超声波处理后加混凝剂比先加混凝剂后用超声波处理的COD和NH3-N的去除率高,分别为61.24%、58.63%。试验表明,先用超声波处理后加混凝剂比先加混凝剂后用超声波处理对COD和NH3-N的去除率高。  相似文献   

8.
超声波+赤霉素处理促进柴胡种子萌发   总被引:1,自引:2,他引:1  
采用饱和D最优设计方法,研究了超声波 赤霉素对柴胡种子萌发的促进效应。结果表明:当超声波处理时间为20~30min,赤霉素浓度32×10~(-6)~59×10~(-6)ml/L时,可明显促进种子的萌发。  相似文献   

9.
王红明  郭素娟  贾汉森 《种子》2015,34(1):19-24
采用培养皿纸上发芽法,研究不同时间(0 min、5 min、10 min、15 min、20 min、30 min)超声波处理对6种源红砂种子萌发的影响,通过对红砂种子发芽率、发芽势、发芽指数和平均发芽时间4个指标进行分析,旨在探寻超声波处理促进红砂种子萌发的最佳时间。结果表明:超声波处理对发芽率的影响不显著,但对发芽势、发芽指数和平均发芽时间影响显著。除种源5外,15 min超声波处理对所有种源均有促进作用,效果最好;但当处理时间超过20 min时,超声波的促进作用会减弱。对不同种源种子发芽率、发芽势的作用结果为:武威民勤>张掖肃州>兰州交大后山>酒泉金塔>张掖山丹>兰州九州台,且各种源之间差异显著。综合各项指标可知,武威民勤的种源最好。  相似文献   

10.
针对设施葡萄生产中光温逆境导致果实品质欠佳(着色差)的问题,对2个葡萄园转色期的‘巨峰’和‘巨玫瑰’葡萄进行每日补光试验处理(4:00—5:00和19:00—22:00),观察处理前后果实品质的差异。结果表明,每日补光4 h处理后‘巨峰’和‘巨玫瑰’着色更深、更均匀,且补光处理能显著提高葡萄果实的颜色指数,补光处理后葡萄果皮的花色苷含量提高了5.00~19.42 µg/g;葡萄果实的可溶性固形物含量增加了0.5~1.7 °Brix,葡萄糖含量增加了0.42~3.43 mg/g,最终成熟期提早了5~7天。综合试验结果可知,在葡萄转色期进行补光处理,可以明显改善红色系葡萄的着色问题,提高葡萄品质,对优质葡萄生产有良好的应用价值。  相似文献   

11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans.  相似文献   

12.
Autotoxicity restricts reseeding of alfalfa (Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments. A series of aqueous extracts from leaves, stems, roots and seeds of alfalfa ‘Vernal’ were bioassayed against alfalfa seedlings of the same cultivar to determine their autotoxicity. The highest inhibition was found in the extracts from the leaves. Extracts at 40 g dry tissue l?1 from alfalfa leaves were 15.4, 17.5 and 28.7 times more toxic to alfalfa root growth than were those from roots, stems and seeds, respectively. A high‐performance liquid chromatography (HPLC) analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compounds depended on the plant parts. In leaf extracts that showed the most inhibitory effect on root growth, the highest amounts of allelochemicals were detected. Among nine phenolic compounds assayed for their phytotoxicity on root growth of alfalfa, coumarin, trans‐cinnamic acid and o‐coumaric acid at 10?3 m were most inhibitory. The type and amount of causative allelochemicals found in alfalfa plant parts were highly correlated with the results of the bioassay, indicating that the autotoxic effects of alfalfa plant parts significantly differed.  相似文献   

13.
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol...  相似文献   

14.
Development of onion (Allium cepa L., cv. ‘Early Cream Gold’) seed under cool climate conditions in Tasmania, Australia occurred over a longer duration than previously reported, but similar patterns of change in yield components were recorded. In contrast to previous studies, umbel moisture content declined from 85 to 67 % over 57 days while seed moisture content decreased from 85 to 31 %. Seed yield continued to increase over the duration of crop development, with increasing seed weight compensating for seed loss resulting from capsule dehiscence in the later stages of maturation. Germination percentage was high and did not vary significantly from 53 to 77 days after full bloom (DAF), but mean germination time declined and uniformity of germination increased significantly over the same time period. The percentage abnormal seedlings declined with later harvest date, resulting in highest seed quality at 77 DAF. The results of this study suggest that the decision to harvest cool climate onion seed crops before capsule dehiscence will result in a loss of potential seed yield and quality.  相似文献   

15.
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination...  相似文献   

16.
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献   

17.
T. Visser  E. H. Oost 《Euphytica》1981,30(1):65-70
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed.  相似文献   

18.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho...  相似文献   

19.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf 1) and recessive for the other (Rf 2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph 3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf 1(one of two dominant additive genes controlling early season leafing out) indicated that Rf 1and Rf 2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf 1, and 0.15 for Lf 1-Sph 3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph 3.It is suggested that competitive disadvantage of lf 1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph 3. Poor performance of lf 1- (and possibly lf 2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off).  相似文献   

20.
Parasitic angiosperms cause great losses in many important crops under different climatic conditions and soil types. The most widespread and important parasitic angiosperms belong to the genera Orobanche, Striga, and Cuscuta. The most important economical hosts belong to the Poaceae, Asteraceae, Solanaceae, Cucurbitaceae, and Fabaceae. Although some resistant cultivars have been identified in several crops, great gaps exist in our knowledge of the parasites and the genetic basis of the resistance, as well as the availability of in vitro screening techniques. Screening techniques are based on reactions of the host root or foliage. In vitro or greenhouse screening methods based on the reaction of root and/or foliar tissues are usually superior to field screenings and can be used with many species. To utilize them in plant breeding, it is necessary to demonstrate a strong correlation between in vitro and field data. The correlation should be calculated for every environment in which selection is practiced. Using biochemical analysis as a screening technique has had limited success. The reason seems to be the complex host-parasite interactions which lead to germination, rhizotropism, infection, and growth of the parasite. Germination results from chemicals produced by the host. Resistance is only available in a small group of crops. Resistance has been found in cultivated, primitive and wild forms, depending on the specific host-parasite system. An additional problem is the existence of pathotypes in the parasites. Inheritance of host resistance is usually polygenic and its transfer is slow and tedious. Molecular techniques have yet to be used to locate resistance to parasitic angiosperms. While intensifying the search for genes that control resistance to specific parasitic angiosperms, the best strategy to screen for resistance is to improve the already existing in vitro or greenhouse screening techniques.  相似文献   

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