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为了研究类胰岛素生长因子1(IGF-1)和类胰岛素生长因子1受体(IGF-1R)基因在绒山羊绒毛生长不同阶段皮肤组织中的表达差异,试验采用实时荧光定量PCR技术对非产绒期和产绒期辽宁绒山羊皮肤中IGF-1和IGF-1R基因的表达进行了测定。结果表明:辽宁绒山羊皮肤中IGF-1和IGF-1R mRNA的表达量在产绒期均极显著高于非产绒期(P<0.01),产绒期IGF-1、IGF-1RmRNA的表达水平分别是非产绒期的8.30倍和7.49倍。说明IGF-1和IGF-1R基因可能与山羊绒周期性生长有关。 相似文献
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胰岛素样生长因子(insulin-like growth factor，IGF)于1978年被正式命名，并且明确了它由IGF-1和IGF-2两部分组成。IGF-1是一种受生长激素调节的单链多肽，由70个氨基酸组成，分子量为7649，其结构有45％与胰岛素相似。IGF-1有种系稳定性，所有哺乳类动物的IGF-1结构与组成基本相同。人类IGF-1的基因位于第12号染色体上，由6个外显子和多个内含子构成，可在全身各处表 相似文献
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选取规模化养殖场正常分娩奶牛54头,按犊牛初生质量划分为A组(≤40kg)9头、B组(40~45kg)25头、C组(≥45kg)20头共3组,母牛分娩后立即采集脐静脉血,ELISA法检测血液脂联素、胰岛素和胰岛素样生长因子-1(IGF-1)含量,分析其间的相关性及与犊牛初生质量的相关性。结果表明,3组脐静脉血中均有脂联素、胰岛素和IGF-1表达,随犊牛初生质量增加,脐静脉血中脂联素、胰岛素和IGF-1含量均逐渐增加。其中A组脂联素含量显著低于C组(P0.05),A、B组和B、C组脂联素含量均分别差异不显著(P0.05);A、B组胰岛素、IGF-1含量均分别显著低于C组(P0.05);A、B组胰岛素、IGF-1含量均分别差异不显著(P0.05);脐静脉血脂联素、胰岛素和IGF-1含量均分别与犊牛初生质量呈极显著性正相关(P0.01);脐静脉血脂联素、胰岛素和IGF-1含量两两间均呈极显著性正相关(P0.01);公犊牛脐静脉血脂联素和胰岛素含量均略高于母犊牛脐静脉血,而公犊牛脐静脉血IGF-1含量略低于母犊牛脐静脉血,但脐静脉血脂联素、胰岛素、IGF-1含量在公、母犊间差异均分别不显著(P0.05)。据此得出,脐静脉血均有脂联素、胰岛素和IGF-1表达;脐静脉血脂联素、胰岛素和IGF-1含量均对犊牛初生质量影响较大;但脐静脉血脂联素、胰岛素、IGF-1含量在公、母犊间差异性均不显著;为进一步研究脐静脉血脂联素、胰岛素和IGF-1对犊牛宫内生长发育的影响机理提供参考。 相似文献
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1生长因子
1．1胰岛素样生长因子
胰岛素样生长因子（IGF）包括IGF-Ⅰ和IGF-Ⅱ，是一类广谱的促生长因子，化学结构与胰岛素类似，为同源的单链多肽。生长激素（GH）的许多生理学作用是间接的，期间主要经由IGF—Ⅰ介导。IGF—Ⅰ的作用由一系列至少6种结合蛋白质所调节。 相似文献
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为了解国内、外两类猪种肌肉生长发育及血清中相关细胞因子含量差异,以我国特有的藏猪和国外杜洛克猪作为研究对象,采用酶联免疫分析法(ELISA)测定6月龄藏猪、杜洛克猪血清中胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)的含量及肌纤维面积(CSA)。结果显示:藏猪血清中IGF-1含量及CSA均极显著低于杜洛克猪(P0.01),IGFBP-3含量极显著高于杜洛克猪(P0.01);CSA与IGF-1含量间呈极显著正相关,与IGFBP-3间呈极显著负相关,说明IGF-1对猪背最长肌生长发育有促进作用,而IGFBP-3可能有抑制作用。 相似文献
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《中国兽医学报》2014,(6):1005-1011
为了研究卵巢摘除对布尔山羊杂种母羊类胰岛素样生长因子1(ginsulin-like growth factor 1,IGF-1)和类胰岛素样生长因子1受体(ginsulin-like growth factor 1receptor,IGF-1R)基因表达的影响。本试验将体质量相近的5月龄布尔山羊杂种母羊作为研究对象,随机分为处理组和对照组,每组20只。试验开始时摘除处理组母羊的卵巢,对照组不摘除。饲养50d后,利用实时荧光定量PCR(qRT-PCR)相对定量方法检测肝脏、背最长肌、股二头肌和肾周脂肪中IGF-1和IGF-1R基因mRNA的相对表达量。卵巢摘除后母羊背最长肌和肝脏组织中IGF-1基因mRNA的相对表达量分别是对照组的6.19倍和6.01倍(P<0.01);IGF-1R基因表达量分别是对照组的11.86倍和9.96倍(P<0.01)。处理组母羊股二头肌中IGF-1基因mRNA的相对表达量与对照组差异不显著(P>0.05);IGF-1R基因是对照组的4.86倍,且差异显著(P<0.05)。在肾周脂肪组织中,处理组母羊IGF-1基因和IGF-1R基因mRNA的相对表达量较对照组增加,但差异均不显著(P>0.05)。由此可见,卵巢摘除可以上调背最长肌和肝脏中IGF-I和IGF-IR基因的表达以及股二头肌中IGF-IR基因的表达。 相似文献
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Stimulation of pig growth performance by porcine growth hormone and growth hormone-releasing factor 总被引：10，自引：0，他引：10
T D Etherton J P Wiggins C S Chung C M Evock J F Rebhun P E Walton 《Journal of animal science》1986,63(5):1389-1399
The current study was undertaken to determine the effects of human growth hormone-releasing factor [hpGRF-(1-44)-NH2] on growth performance in pigs and whether this response was comparable to exogenous porcine growth hormone (pGH) treatment. Preliminary studies were conducted to determine if GRF increased plasma GH concentration after iv and im injection and the nature of the dose response. Growth hormone-releasing factor stimulated the release of pGH in a dose-dependent fashion, although the individual responses varied widely among pigs. The results from the im study were used to determine the dose of GRF to use for a 30-d growth trial. Thirty-six Yorkshire-Duroc barrows (initial wt 50 kg) were randomly allotted to one of three experimental groups (C = control, GRF and pGH). Pigs were treated daily with 30 micrograms of GRF/kg body weight by im injection in the neck. Pigs treated with pGH were also given 30 micrograms/kg body weight by im injection. Growth rate was increased 10% by pGH vs C pigs (P less than .05). Growth rate was not affected by GRF; however, hot and chilled carcass weights were increased 5% vs C pigs (P less than .05). On an absolute basis, adipose tissue mass was unaffected by pGH or GRF. Carcass lipid (percent of soft-tissue mass) was decreased 13% by GRF (P less than .05) and 18% by pGH (P less than .05). Muscle mass was significantly increased by pGH but not by GRF. There was a trend for feed efficiency to be improved by GRF; however, this was not different from control pigs. In contrast, pGH increased feed efficiency 19% vs control pigs (P less than .05). Chronic administration of GRF increased anterior pituitary weight but did not affect pituitary GH content or concentration. When blood was taken 3 h post-injection, both GRF- and pGH-treated pigs had lower blood-urea nitrogen concentrations. Serum glucose was significantly elevated by both GRF and pGH treatment. This was associated with an elevation in serum insulin. These results indicate that increasing the GH concentration in blood by either exogenous GH or GRF enhances growth performance. The effects of pGH were more marked than for GRF. Further studies are needed to determine the optimal dose of GRF to administer in growth trials and the appropriate pattern of GRF administration in order to determine whether GRF will enhance pig growth performance to the extent that exogenous pGH does. 相似文献
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Stimulation of swine growth by porcine growth hormone 总被引：7，自引：0，他引：7
Highly purified porcine growth hormone (pGH; USDA-B1) was administered by im injection (22 micrograms X kg body weight-1 X d-1) to rapidly growing Yorkshire barrows for 30 d. Growth hormone significantly increased growth rate (10%), feed efficiency (4%), cartilage growth and muscle mass. However, pGH did not affect carcass adipose tissue mass. Intramuscular lipid content of the longissimus was increased 50% by pGH administration. Plasma pGH concentration was elevated (7- to 11-fold) for 3 to 5 h post-injection. Chronic administration of pGH depressed pituitary GH content and concentration approximately 45%. No GH antibodies were detected in the plasma of GH-treated swine. Plasma somatomedin-C concentration was increased 55% by GH treatment 3 h post-injection. Plasma glucose and insulin concentrations were both significantly increased in GH-treated swine, suggesting that the animals had developed a state of insulin resistance. Plasma-free fatty acid concentration tended to be higher in GH-treated animals. Treatment of swine with pGH significantly decreased plasma blood urea nitrogen. Assessment of animal health during the trial and postmortem indicated that pGH administration did not have any adverse effects. In summary, treatment of young, rapidly growing swine with pGH stimulated growth performance without affecting animal health or inducing the production of GH antibodies. 相似文献
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1. The concentrations of circulating GH were low in 1‐week‐old birds (male plasma pool 30 ng/ml, female 32 ng/ml), reached a maximum at 7 weeks in male birds (142 ± 26 SEM ng/ml) or 4 weeks in females (185 ± 32 ng/ml) and then decreased to 17.3 ± 2–8 ng/ml in males and 8?7 ± 0–6 ng/ml in females at 17 weeks.
2. Significant inverse correlations between GH concentration and age or body weight were found (male, r = —0–693), female, r = —0–623).
3. In males, but not females, the weekly increase in body weight was correlated with the plasma GH concentration (r = 0–291). 相似文献
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Kitagawa H Kitoh K Ito T Ohba Y Nishii N Katoh K Obara Y Motoi Y Sasaki Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):167-170
Serum concentrations of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were determined in 5 calves in the same lineage with growth retardation. They had normal appetites, activities, body proportion, and laboratory test results. Calves with growth retardation had higher serum GH concentrations and lower serum IGF-I concentrations. These findings suggested defects in the GH-IGF-1 axis, such as in the GH-receptor. 相似文献
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E E Connor S M Barao L W Douglass S A Zinn G E Dahl 《Journal of animal science》1999,77(10):2736-2741
Development of practical, physiologically based methods that provide an early, yet accurate, evaluation of a bull's genetic merit could benefit the beef industry. The use of GH response to a single, acute dose of GHRH was evaluated as a predictor of future growth performance and carcass characteristics of weanling bulls. Fifty-six Angus bulls averaging 229 d (SD = 27) of age were administered three doses i.v. (0, 1.5, and 4.5 microg/100 kg BW) of human GHRH (1-29) analog in a Latin square design balanced for residual effects. Blood samples were collected via jugular catheter at -60, -45, -30, -15, 0, 5, 10, 15, 30, 45, 60, 90 and 120 min relative to GHRH injection. Serum concentrations of GH were plotted over time. Response to GHRH was calculated as the area under the GH response curve (AUC-GH) using the trapezoidal approximation. Relationships between AUC-GH, weaning weight adjusted to 205 d of age (205-d WW), and direct weaning weight EPD (WWEPD) versus age-adjusted BW (BWadj), ADG, and carcass measurements from a 140-d growth performance test were evaluated using simple linear regression. A positive correlation between AUC-GH and ADG and an inverse relationship between AUC-GH and carcass fat were observed. The present study provides evidence that AUC-GH is a better predictor of future growth performance in beef bulls than 205-d WW or WWEPD values. Thus, GH response to GHRH is associated with subsequent growth and may be a useful tool for sire selection in beef production. 相似文献
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T L Auchtung E E Connor S M Barao L W Douglass G E Dahl 《Journal of animal science》2001,79(6):1566-1572
The response of GH to GHRH at weaning is known to predict postweaning growth and body composition in beef bulls. The objective of this study was to determine whether GH response to a challenge of GHRH and plasma IGF-I can predict growth rate and body composition in the beef heifer. Growth hormone response to a challenge with two doses of GHRH was measured in 67 Angus heifers averaging 225 d of age (SD = 21) and 217 kg BW (SD = 32). Blood samples were collected at 0 and 10 min relative to an initial "clearance dose" (4.5 micrograms GHRH/100 kg BW) and again, 3 h later, relative to a challenge dose (1.5 or 4.5 micrograms GHRH/100 kg BW). Each animal received each of the two challenge doses, which were randomly assigned across 2 d of blood collection. Serum GH concentration was measured by RIA. Plasma was collected every 28 d during a 140-d growth test and assayed for IGF-I by RIA. Body weight was measured every 28 d and hip height was measured at weaning and at the end of a 140-d growth test. Average daily gain was calculated on d 140 of the growth test and body composition measurements were estimated by ultrasound 2 wk after completion of the growth test. Responses to the two GHRH challenges were dose-dependent (P < 0.05). Average daily gain tended to be related to GH response to the 1.5 micrograms GHRH/100 kg BW dose (R2 = 0.05; P = 0.06), but no relationship was observed at the 4.5 micrograms GHRH/100 kg BW dose (R2 = 0.00; P = 0.93). An inverse relationship (R2 = 0.06; P = 0.02) was observed between response to the 1.5 micrograms GHRH/100 kg BW dose and intramuscular fat percentage. Mean plasma IGF-I concentration was positively associated with ADG (R2 = 0.06; P < 0.01). Growth hormone response to GHRH is modestly related to body composition but not to ADG in weanling beef heifers and likely has limited use in evaluation of growth performance in replacement beef heifers. 相似文献
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