共查询到18条相似文献，搜索用时 221 毫秒
1.
《中国兽医学报》2019,(2):307-312
为了探讨乌头碱对小鼠血液生理指标的影响,将120只小鼠随机分成4组,即对照组(生理盐水)、低剂量组(0.14μmol/L)、中剂量组(0.28μmol/L)、高剂量组(0.56μmol/L),连续灌胃30d。分别在10,20,30d每次每组随机采集10只小鼠血液,用全自动血液细胞分析仪测定血液中白细胞(WBC)、淋巴细胞(LymPh)、单核细胞(Mon)、中心粒细胞(Gran)、红细胞(RBC)、血红蛋白(HGB)、红细胞压积(HCT)和血小板(PLT)的含量。结果显示,高剂量组小鼠肝脏、肺脏、大脑的脏器系数明显增加(P0.05或P0.01)。随毒物浓度的增加,各染毒组WBC、LymPh、Mon、Gran先升高后降低,与对照组相比,高剂量组变化最为明显,且差异显著(P0.05或P0.01)。RBC、HGB、HCT和PLT持续降低,在20和30d时,除低剂量组外,差异显著(P0.05或P0.01)。结果表明,不同浓度乌头碱对小鼠血液生理指标有显著影响,且存在明显时间和剂量效应关系,可引起动物贫血,降低机体免疫机能。 相似文献
2.
《草业与畜牧》2016,(1)
试验以四川省的1个西藏羊地方类群―贾洛羊为研究对象,选择其12月龄的公、母羊各5只,测定其生理指标、血液生理指标及血液生化指标。结果表明:12月龄贾洛羊的体温、呼吸频率及心率等生理指标在公、母羊间无显著差异;母羊的WBC血液生理指标显著高于羔羊(0.01P0.05),RBC、HGB、HCT、MCV、MCH、MCHC、PLT、RDW-SD、MPV、PCT、PDW和RDW-CV等指标在公、母羊间差异不显著(P0.05);ALT、AST、TP、ALB、GLO、ALP、LDH、A/G、GLU、CHE和CHOL等血液生化指标在公、母羊间无显著差异(P0.05)。试验建立了12月龄段贾洛羊的生理指标和血液生理生化指标的参考值,为以后加强贾洛羊的饲养管理、疾病诊断、适应性及选育等提供参考依据。 相似文献
3.
《草业与畜牧》2017,(2)
试验以四川省的1个西藏羊地方类群!贾洛羊为研究对象,选择其24月龄的公、母羊各5只,测定其生理指标、血液生理指标及血液生化指标。结果表明:24月龄贾洛羊的体温、呼吸频率及心率等生理指标在公、母羊间无显著差异;母羊的MPV血液生理指标显著高于公羊(0.01P0.05),公羊的PDW指标显著高于母羊(0.01P0.05),WBC、RBC、HGB、HCT、MCV、MCH、MCHC、PLT、RDW-SD、PCT和RDW-CV等指标在公、母羊间差异不显著(P0.05);ALT、AST、TP、ALB、GLO、ALP、LDH、A/G、GLU、CHE和CHOL等血液生化指标在公、母羊间差异不显著(P0.05)。试验建立了24月龄段贾洛羊的生理指标和血液生理生化指标的参考值,为以后加强贾洛羊的饲养管理、疾病诊断、适应性及选育等提供参考依据。 相似文献
4.
南非海狮是目前国内外动物园、水族馆中饲养比较普遍的动物,但经常出现一些细菌性、病毒性和寄生虫等疾病,在海狮健康状况监测和疾病诊断中需要有生理生化指标正常值作为参考。但目前南非海狮的正常生理生化指标报道较少。杭州动物园先后对6只成年南非海狮进行采血,共收集20个（12雄8雌）血液常规样本测定了15项主要生理指标和6个（4雄2雌）血清生化标本对18项主要生化指标进行检测。在测定项目中WBC、RBC、MCH、MCV、RDW、HCT、MPV、PCT、ALT、TP、ALB、TBIL、GLU、Ca2＋、Pi、Mg2＋、K＋、Na＋测定值个体间差异不大,而HGB、MCHC、PLT、GGT、ALP、Cr、UA测定值个体间差异较大。在血液常规指标中只有HGB和Lymph（％）雌雄间的差异显著（P〈0．05）。WBC、HGB、PIJT的测定值与斑海豹报道的参考值比较接近,红细胞大小、MCV与人的参考值较接近。这些指标的测定对于海狮的健康状况监测和疾病诊断等工作都将有一定的参考价值。 相似文献
5.
为判定肾功能衰竭病原及引起的相关症状，并提供合理的诊治方案，本研究对一例柯基病犬进行表征、血常规、血液生化、血气及病原进行检测。结果显示，与正常参考值相比，病犬前期血常规检测中淋巴细胞比例（W-SCR）、中间细胞比例（W-MID）、中间细胞（MID）及红细胞分布宽度（RDW-CV）升高；粒细胞比例（W-LCR）、红细胞压积（PCV）、红细胞平均体积（MCV）、血红蛋白含量（MCH）、红细胞分布宽度（RDW-SD）明显降低。与正常参考值相比，生化指标中钙离子（Ca2+）、血糖（GLU）、尿素氮（BUN）、血磷（PHOS）、胰淀粉酶（AMYL）及肌酐（CRE）明显升高。血气指标中GLU、BUN、钾离子（K+）均比参考值高；钠离子（Na+）、氯离子（Cl-）、二氧化碳分压（PCO2）比参考值降低。犬钩端螺旋体抗原检测板检测结果为阳性。治疗后血常规中白细胞总数（WBC）、淋巴细胞（SCR）比正常参考值明显升高；红细胞总数（RBC）、血红蛋白（HGB）及血小板总数（PLT）则比参考值降低。经Hotelling T2对治疗前后各指标进行多变量分析，结果F=1 905 045.32，P=0.001，差异极显著；单变量检测指标中WBC、W-LCR、SCR、MID、HGB、PCV、MCV、RDW-SD差异极显著（P<0.01），W-MID、RBC、MCH及血小板分布宽度（PDW）差异显著（P<0.05）。治疗后生化检测指标中，与正常参考值相比，多数指标在正常值范围内。治疗后期病犬精神好转，经Hotelling T2与治疗前差异性分析，多变量F=85.21，P=0.081，差异不显著；单变量检验指标GLU、BUN、PHOS、AMYL及CRE差异极显著（P<0.01）。治疗后期血气指标中GLU、BUN、K+均比参考值高；Na+、Cl-及PCO2比参考值低，对治疗前后血气指标进行Hotelling T2差异性分析，多变量检验结果F=7 978.48，P=0.008，差异极显著；单变量检验结果中，GLU和BUN差异极显著（P<0.01）。治疗方案中，采用利尿、抗生素药物，附加补充电解质、能量、维生素及CoA等综合输液治疗，7 d后，病犬精神好转，机体机能基本恢复正常，转归期部分指标的升高属于机体机能恢复健康的一种正常体现。说明治疗方案正确，综合体液疗法是治疗该病引起肾功能衰竭有效的方法。 相似文献
6.
试验测定20、40、60周龄矮小型褐壳蛋鸡血液生理指标、血清生化指标,每一周龄公、母鸡各9只,样本量为54只。将数据进行t检验及多重比较分析,结果表明,在此3个时间点上,指标RBC、HGB、MPV、PDW、LYMF%、TP和CHO呈现上升趋势,指标HCT、MCV、RDW%、WBC、LYMF值是先下降后上升,而MCH、MCHC、PCT、PLT、MID、MID%、GRAN、GRAN%、AST、ALB、TG、GLU和ALT值是先上升后下降。同时,不同周龄鸡之间在血液生理生化指标上表现出不同项目差异显著(P0.05)。20、40及60周龄矮小型褐壳蛋鸡雌、雄间均存在显著差异(P0.05)的血液生理生化指标有RBC、HCT、HGB、WBC、LYMF和AST,这表明矮小型褐壳蛋鸡的生理和生化指标随周龄不同,在雌、雄之间的差异随之发生变化。 相似文献
7.
为了研究低剂量汞对SD大鼠血液生理生化指标的影响,24只SD大鼠被随机分为4组,分别为对照组(蒸馏水),汞低剂量组(50 mg/L),汞中剂量组(100 mg/L),汞高剂量组(200 mg/L),采用自由饮水染毒,染毒时间为21d.运用全自动血液细胞分析仪测定RBC、WBC、HGB、HCT、MCV、MCH、MCHC、PLT等血液生理指标的变化;采用半自动生化分析仪测定汞对大鼠血清生化指标TP、ALB、BUN、CR、GLU、TBIL、TCH、TG含量和ALT、AST活性的影响.结果显示,随染毒浓度的增加,各染毒组WBC呈升高趋势,与对照组相比,差异显著(P<0.05);而RBC、HGB、HCT、MCV、MCH、MCHC和PLT则呈降低趋势,部分组间差异显著(P<0.05);GLU、ALB和TP含量呈降低趋势(P<0.05或P>0.05),而CR、BUN和TBIL含量及ALT、AST活性呈升高趋势(P<0.05或P>0.05),此外,当汞染毒剂量在50、100 mg/L时,TG和TCH含量呈升高趋势,而在200 mg/L时则显著降低,与对照组比较,均有显著差异(P<0.05).低剂量汞暴露对SD大鼠血液生理生化指标有显著影响,且存在明显剂量-效应关系;低剂量汞暴露主要引起SD大鼠小细胞低色素性贫血及不同程度肝、肾损伤. 相似文献
8.
本文主要研究当归补血汤对免疫抑制小鼠造血功能和免疫功能的影响。用环磷酰胺(Cy)构建小鼠免疫抑制病理模型,然后分别给正常小鼠和环磷酰胺所致免疫抑制小鼠饮服自制中药免疫调节剂——当归补血汤、玉屏风口服液及黄芪多糖,检测参试药物对不同免疫状态下的小鼠血液学指标如白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白(HGB)、血小板计数(PLT)以及体重、脾脏指数、肝脏指数的影响。结果显示:当归补血汤、玉屏风口服液和黄芪多糖对正常小鼠的血液学指标(WBC、RBC、HGB、PLT)及肝、脾指数没有显著影响,但对Cy造模小鼠的血液学指标有一定程度的恢复作用,且当归补血汤效果优于玉屏风口服液和黄芪多糖(P<0.05),但均未能使WBC、RBC、HGB、PLT值恢复到正常水平(P>0.05);各免疫调节剂对Cy造成的小鼠增重减少和脾脏萎缩有明显的抵抗作用,且当归补血汤可将脾脏的损伤恢复到正常水平(P<0.05);各免疫调节剂均不能将体重的增加恢复到正常水平,且对肝脏肿大无明显拮抗作用。 相似文献
9.
为了比较大耳白兔和青紫蓝兔体温、血常规和血气指标的差异,试验将60只家兔按品种分为2组,每组30只。每天上午、下午分别测定体温1次,连续5 d,试验第5天每组随机抽取10只,心脏采血,用全自动血细胞分析仪测定白细胞数(WBC)、红细胞数(RBC)和血小板(PLT)等16项血常规指标,用血气分析仪测定血清主要离子、血液酸碱度(pH值)和血氧饱和度(SO2)等10项血气指标,并进行比较。结果表明:2组家兔的体温和血气指标差异不显著(P>0.05),血常规指标中除大耳白兔粒细胞数和粒细胞百分率显著高于青紫蓝兔(P<0.05)外,其余血常规指标差异不显著(P>0.05)。说明大耳白兔和青紫蓝兔体温、血气和血常规指标相差不大。 相似文献
10.
采用BC-2600Vet兽用全自动血液细胞分析仪,对120例犬瘟热病犬进行季节、年龄及血液诊断指标分析.试验按年龄分为幼龄、育成、成年3个组,主要检测HGB、RBC、WBC、MCV、MCH、MCHC、HCT及GRAN、LYMPH、PLT等血液学指标.1~3月份发病占17.5%、4~6月份发病占32.5%、7~9月份发病占24.2%、10~12月份发病占25.8%.120例病犬,以2~4月龄发病最多,10月龄最低.病犬血液学检测指标在幼龄(1~5月龄)、育成(6~12月龄)和成年(13月龄以上)三个发育时期,与对照组比较13项血液学诊断指标均具有临床诊断价值,患病犬血液学检测的各项指标均低于(P＜0.05)对照组;在幼龄、育成和成年三个发育时期之间,RBC、WBC和HGB等13项血液学诊断指标亦有明显的差异(P＜0.05). 相似文献
11.
Roleff S Arndt G Bottema B Junker L Grabner A Kohn B 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(2):155-166
BACKGROUND: The CA530-VET is a completely automated impedance cell hematology analyzer, which yields a 16-parameter blood count including a 3-part leukocyte differential. OBJECTIVES: The aim of this study was to examine the operational potential of the CA530-VET and its value for use in veterinary practice. METHODS: The analyzer was tested for blood carry-over, precision, and accuracy. Comparison methods included the CELL-DYN 3500, microhematocrit centrifugation, manual platelet (PLT) counting for feline and equine species, and a 100-cell manual WBC differential. Blood samples for comparison of the methods were obtained from 242 dogs, 166 cats, and 144 horses. RESULTS: The carry-over ratio (K) was 0.28% for RBC, 0.59% for PLT, 0.32% for WBC, and 0.18% for hemoglobin (HGB) concentration. Coefficients of variation (CVs) for within-batch precision and duplicate measurement of blood samples were clearly within the required limits, except for duplicate platelet counts in cats (8.7%) and horses (9.5%). The WBC count was in excellent agreement for dogs and horses and RBC count was in excellent agreement for horses. The accuracy of feline WBC counts was not acceptable, with the exception of values at the high end of the range. RBC counts in dogs and cats, and HGB concentration and MCV in all 3 species were sufficiently accurate. The CA530-VET HCT results were in excellent agreement with microhematocrit results in horses but exceeded the maximum allowed inaccuracy for cats and dogs. In all species, PLT counts established mechanically and manually were not in adequate agreement. Large differences were found between the CA530-VET and the manual differential percentage for lymphocytes and "mid-sized cells" (monocytes and basophilic granulocytes). CONCLUSIONS: The CA530-VET can be considered useful for routine canine, feline, and equine blood cell analyses. It should not be considered accurate, however, for PLT counts, feline total WBC counts in the subnormal and normal range, and leukocyte differentials, except for granulocytes. 相似文献
12.
为观察犬埃立克体病急性期血液学变化,对6只人工感染埃立克体的比格犬进行了血常规及血生化检测.采集比格犬感染前后的血液样本,测定血常规及各项血生化指标,并对测定结果作统计学处理.血常规检测发现感染后RBC、WBC、HGB、HCT、PLT、MPV、LYM和MONO值极显著降低(P＜0.01),RDW显著降低(P＜0.05);血生化检测发现感染后ALT和AST极显著升高(P＜0.01),ALB极显著降低(P＜0.01).结果表明,我国犬埃立克体病急性期血液学变化较国外报道的更为明显,这可能与埃立克体中国病原株的致病性不同有关. 相似文献
13.
本研究测定了石林龙晖野生动物科研中心饲养的非洲白犀牛的红细胞数、血红蛋白、红细胞比积、平均红细胞体积、平均血红蛋白含量、平均血红蛋白浓度、红细胞宽度变异系数、红细胞宽度标准差、血小板数、平均血小板体积、白细胞数、淋巴细胞绝对值、中间细胞绝对值、粒细胞绝对值、淋巴细胞百分比、中间细胞百分比、粒细胞百分比、大血小板百分比、血小板比积、血小板分布宽度。通过比较分析,雌、雄犀牛在红细胞数、大血小板百分比差异性显著（O．01〈P〈0．05）。其他雌、雄犀牛各项参数差异性不显著（P〉0．05）。雄性白犀牛与公乌蒙马相比,二者的血红蛋白差异性不显著。雌性白犀牛血液中的红细胞数与母乌蒙马的红细胞数差异性不显著。雌、雄白犀牛与安西牛的红细胞数和血红蛋白差异性不显著。因此,在诊断白犀牛血液常规指标时可参考乌蒙马和安西牛相关血液常规指标。 相似文献
14.
Bourgès-Abella N Geffré A Concordet D Braun JP Trumel C 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(3):303-315
Background: The laser‐based Sysmex XT‐2000iV hematology analyzer is increasingly used in veterinary clinical pathology laboratories, and instrument‐specific reference intervals for dogs are not available. Objective: The purpose of this study was to establish canine hematologic reference intervals according to International Federation of Clinical Chemistry and Clinical and Laboratory Standards Institute guidelines using the Sysmex XT‐2000iV hematology analyzer. Methods: Blood samples from 132 healthy purebred dogs from France, selected to represent the most prevalent canine breeds in France, were analyzed. Blood smears were scored for platelet (PLT) aggregates. Reference intervals were established using the nonparametric method. PLT and RBC counts obtained by impedance and optical methods were compared. Effects of sex and age on reference intervals were determined. Results: The correlation between impedance (I) and optical (O) measurements of RBC and PLT counts was excellent (Pearson r=.99 and .98, respectively); however, there were significant differences between the 2 methods (Student's paired t‐test, P<.0001). Differences between sexes were not significant except for HCT, PLT‐I, and PLT‐O. WBC, lymphocyte, and neutrophil counts decreased significantly with age (ANOVA, P<.05). Median eosinophil counts were higher in Brittany Spaniels (1.87 × 109/L), Rottweilers (1.41 × 109/L), and German Shepherd dogs (1.38 × 109/L) than in the overall population (0.9 × 109/L). PLT aggregates were responsible for lower PLT counts by the impedance, but not the optical, method. Conclusion: Reference intervals for hematologic analytes and indices were determined under controlled preanalytical and analytical conditions for a well‐characterized population of dogs according to international recommendations. 相似文献
15.
To determine the pathogen and related symptoms caused by renal failure,and to provide a reasonable diagnosis and treatment plan,a case of disease on Welsh Corgi dog was tested by the method of symptoms,blood routine examination,blood biochemical test,blood gas analysis and identification of pathogen.The results showed that these data of W-SCR,W-MID,MID and RDW-CV rose than normal reference index in blood routine examination before the hospitalization,respectively.Meanwhile,data of W-LCR,PCV,MCV,MCH and RDW-SD had reduced separately.These data of Ca2+,GLU,BUN,PHOS,AMYL and CRE were up than normal reference index in blood biochemical test,respectively.These data of GLU,BUN and K+ had risen respectively,nevertheless,these data of Na+,Cl- and PCO2 had lowered separately.The result was positive by the antigen testing with the Leptospira detector plate.After treatment with synthetical therapy,WBC and SCR had risen than normal references index,and data of RBC,HGB and PLT were down respectively.By the Hotelling T2 analysis of the difference between before and after the therapy on blood routine index,it was significant difference in the multivariate test (F=1 905 045.32,P=0.001).These data of WBC,W-LCR,SCR,MID,HGB,PCV,MCV and RDW-SD were extremely significant differences in single variable analysis (P<0.01),and other data with significant differences were W-MID,RBC,MCH and PDW(P<0.05).But,in blood biochemical test,most of data were in normal reference values.The mental state of sick dog was recovering gradually normal than before.Multivariate test was no significant difference by the Hotelling T2 analysis (F=85.21,P=0.081),but other data of GLU,BUN,PHOS,AMYL and CRE were extremely significantly different in single variable analysis (P<0.01).These data of GLU,BUN and K+ were up than after therapy in blood gas analysis,and data of Na+,Cl- and PCO2 reduced respectively.There were extremely significant differences in the multivariate test (F=7 978.48,P=0.008).These data with extremely significant differences were GLU and BUN in single variable analysis(P<0.01).The sick dog was recovering normal after treatment,and mental state went back to normal after 7 days therapy using the drugs of diuretics,antibiotics,rehydration electrolyte,energy medicine,vitamins and CoA.It should be noted that the part of high index of reexamination was a normal manifestation when the body was recovering to normal functioning.The method of synthetical fluid therapy was provided the correctness to treat the renal failure caused by canine Leptospira. 相似文献
16.
本研究对由地锦草、石榴皮等组成的中药复方对大肠杆菌感染雏鸭的临床治疗效果进行了研究，旨在为临床大肠杆菌感染提供新的治疗手段。试验选取50只健康1日龄雏鸭，随机分为5组，每组10只。试验组分别为空白对照组、模型组、阿莫西林组、中药复方高剂量组（DSH）和低剂量组（DSL）。给药组在3日龄开始给药，连续给药至试验结束，共10 d。除空白对照组外，在雏鸭6日龄时用大肠杆菌腹腔注射进行攻毒，0.5 mL/只。比较各试验组雏鸭的病死率；对病死鸭和各组存活鸭进行解剖，观察肝脏和小肠的病理剖检及HE染色切片；检测血常规及血清生化各项指标，评价中药复方对感染大肠杆菌雏鸭的治疗效果。结果显示，DSH、DSL和阿莫西林均能够有效降低感染雏鸭的死亡率；与模型组相比，阿莫西林和DSH、DSL组均可减少肝脏、小肠等器官的损伤。与空白对照组相比，模型组的谷草转氨酶（AST）和谷丙转氨酶（ALT）显著升高（P<0.05），碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和尿素（UREA）极显著升高（P<0.01），且模型组和给药组血液中白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、血小板（PLT）等指标变化显著（P<0.05）。与模型组相比，给药组ALP显著或极显著降低（P<0.05；P<0.01），血液中WBC、RBC、HGB、PLT等指标极显著升高（P<0.01），说明给药组机体损伤程度低于模型组。以上结果表明，中药复方可以减轻因大肠杆菌所引起的机体损伤，且作用效果与阿莫西林相近。 相似文献
17.
QIN Feng LIU Yun WU Zhi FENG Qi WU Shuang SONG Haigang WU Xiaojie ZHU Shanyuan 《中国畜牧兽医》2007,47(10):3371-3378
In this study,the clinical therapeutic effect of Chinese herbal compound composed of Euphorbia humifusa Willd.and Punica granatum L.on ducklings infected with Escherichia coli (E.coli) was studied in order to provide a new therapeutic method for clinical treatment of E.coli.Fifty healthy one-day-old ducklings were selected and randomly divided into five groups,ten ducklings in each group.The experimental groups were blank control group,model group,amoxicillin group,Chinese medicine compound high-dose group(DSH) and low-dose group(DSL).The administration group started administration at 3 days of age,continued administration for 3 days,and continued administration until the end of the experiment,10 days total.Except for control group,when the ducklings were 6 days old,E. coli was intraperitoneally injected for challenge,0.5 mL/head.The mortality of the experimental groups was compared.The dead ducks and the surviving ducks were dissected,the pathological section of liver and small intestine and HE staining sections were observed.The blood routine indexes and serum biochemical analysis were detected to evaluate the therapeutic effect of traditional Chinese medicine compound on E.coli infected ducklings.The results showed that the high and low doses of the traditional Chinese medicine compound and the amoxicillin could effectively reduce the mortality of infected ducklings.Compared with model group,amoxicillin and traditional Chinese medicine high and low dose groups could reduce liver,small intestine and other organs damage.Compared with control group,aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in model group increased significantly (P<0.05),and alkaline phosphatase (ALP),lactate dehydrogenase (LDH),UREA also showed extremely significant upward trend (P<0.01),and the WBC,RBC,HGB,PLT and other indicators changed extremely significantly(P<0.05).Compared with model group,the alkaline phosphatase(ALP)value was significantly or extremely decreased in the administration group(P<0.05;P<0.01),and the blood WBC,RBC,HGB and PLT indexes were extremely significantly increased (P<0.01).The results indicated that the degree of body damage in the administration group was lower than that in the model group. 相似文献
18.
为了观察盐酸多西环素对急性期犬埃立克体病的治疗效果,对人工感染埃立克体的比格犬进行了盐酸多西环素的治疗试验。采集比格犬感染前及治疗前后的血液样本,测定血常规及各项血生化指标,并对测定结果作统计学处理。血常规检测发现与治疗前相比,治疗后RBC、WBC、HGB、PLT、MPV、NEU-TRO、LYM、MONO和RDW显著升高;HCT、MCV、MCH和MCHC无明显变化;血生化检测发现,与治疗前相比,治疗后ALT和AST显著降低,ALB显著升高;治疗后与感染前相比,血常规和血生化各项均没有显著变化。进一步证实了盐酸多西环素对犬埃立克体病急性期病例具有良好的治疗效果。 相似文献