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1.
SC Finch JB Vlaming BL Sutherland C van Koten WJ Mace LR Fletcher 《New Zealand veterinary journal》2018,66(2):93-97
AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.
METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.
RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).
CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated. 相似文献
2.
Amino acid neurotransmitter release from cerebrocortical synaptosomes of sheep with severe ryegrass staggers in New Zealand 总被引：1，自引：0，他引：1
P G Mantle 《Research in veterinary science》1983,34(3):373-375
Enhanced unstimulated release of the excitatory amino acid neurotransmitters aspartic acid and glutamic acid was measured in cerebrocortical synaptosomes prepared from sheep showing severe symptoms of ryegrass staggers. A similar pattern of deviation from neurologically normal animals was seen whether the sheep had contracted the disorder naturally or whether they had been deliberately caused to develop the disorder by grazing specific pastures rich in endophyte infected ryegrass. Deranged neurotransmitter release may reasonably be involved in the chemical pathology of ryegrass staggers and the findings are in accord with the putative synonymy of lolitrem toxicosis and ryegrass staggers. 相似文献
3.
Two herbicides (glyphosate and paraquat) and a plant growth regulator (paclobutrazol) were applied to endophyteinfected (Acremonium lolii) perennial ryegrass swards. Subsamples of these swards were then chemically analysed at intervals up to 28 days later for lolitrem B, the compound responsible for perennial ryegrass staggers in domestic livestock. Glyphosate and paclobutrazol had no effect on lolitrem B concentrations. Paraquat applications decreased lolitrem B concentrations in the herbage. Because none of the chemicals tested increased the concentration of lolitrem B in the herbage, they are unlikely to be directly implicated in perennial ryegrass staggers in grazing animals. 相似文献
4.
Abstract Ryegrass staggers is a seasonal mycotoxicosis of grazing livestock characterised by tremors, in coordination and a staggering gait almost unaccompanied by physical lesions. Deaths occur only as a consequence of accident or starvation. Outbreaks, in summer and autumn, occur only on pasture in which endophyte (Neotyphodium lolii)-infected perennial ryegrass (Lolium perenne) predominates and usually on which animals are grazed intensively. Animals recover when moved to a different type of grazing or after rain has promoted pasture growth. The disease was recognised for 80 years before its cause was discovered as a consequence of a grazing trial of sheep on three ryegrass cultivars which happened to have three different levels of endophyte infection. The endophyte was first formally described as Acremonium loliae, later corrected to Acremonium lolii, and was finally placed in the genus Neotyphodium. It produces a number of secondary metabolites of which lolitrem B is the principal one causing ryegrass staggers symptoms. Ergopeptides are also produced which cause heat stress and lack of productivity. N. lolii is symptomless in the plant, seed borne and grows intercellularly in the aerial parts, mainly in reproductive tillers and leaf sheaths but sparsely in leaf blades. It dies in stored seed and infection rates of different ryegrass cultivars have depended on seed storage times during their production. In addition, N. Lolii produces insect feeding deterrents, among them peramine, which protects infected plants from pest predation. Because of this, control of ryegrass staggers by elimination of endophyte-infected ryegrass is not feasible in areas in which insect predation is a serious pasture problem. However, N. lolii strains vary in the secondary metabolites they produce allowing the selection of strains that produce desirable metabolites. By inoculating such strains into uninfected ryegrass plants it is possible to produce cultivars which do not cause ryegrass staggers but resist insect predation. This review aims to provide a comprehensive summary of the current understanding of the N. lolii / L. perenne symbiosis, the toxins it is known to produce, their effects on animals and plants and the strategies used to control their ill effects while maximising their beneficial ones. 相似文献
5.
Shigeru Miyazaki Ikumi Ishizaki Masumi Ishizaka Tadashi Kanbara Yuriko Ishiguro-Takeda 《Journal of veterinary diagnostic investigation》2004,16(4):340-342
Lolitrems are neurotoxins found in endophyte-infected perennial ryegrass. Lolitrems, primarily lolitrem B, are the causative agents of ryegrass staggers in livestock. To guarantee the safety of meat produced from cattle consuming endophyte-infected perennial ryegrass, lolitrem B concentrations in tissues of Japanese Black cattle were determined using high-performance liquid chromatography. Lolitrem B was not detected in muscle, liver, kidney, or cerebrum of a Japanese Black cow with signs of ryegrass staggers. In contrast, perirenal fat contained 210 ppb lolitrem B. Three cows that received half as much perennial ryegrass straw as the cow with ryegrass staggers showed no clinical signs of ryegrass staggers. However, low concentrations of lolitrem B (less than 150 ppb) were detected in their fat tissue. These observations indicate that human exposure to the neurotoxic effect of lolitrem B through beef is unlikely. The amount of lolitrem B consumed by cattle can be estimated by the determination of lolitrem B in fat tissue. 相似文献
6.
Sir, — We wish to report the isolation of two potent neurotoxins from herbage collected from pastures on which the disease of livestock known as ldryegrass staggers rd(8)(11) occurred. These neurotoxins have been partially characterised by their mass spectral properties (see below) and appear to be new compounds not previously reported. We propose the general name lolitrems for the neurotoxins, based on their association with ryegrass (Lolium perenne L.) and on their ability to produce tremors in animals, and name the isolated compounds lolitrem A and lolitrem B. 相似文献
7.
R.T. Gallagher A.G. Campbell A.D. Hawkes P.T. Holland D.A. McGaveston E.A. Pansier 《New Zealand veterinary journal》2013,61(11):183-184
Abstract Extract Sir:- Recently, two potent neurotoxins named lolitrem A and lolitrem B were isolated from herbage collected from pastures on which the livestock disorder ryegrass staggers (RGS) occurred. et al.(1) We now wish to report that samples of perennial rygrass (Loliun yeretzrze L.) seed obtained from several locations in New Zealand contained potent neurotoxins which include lolitrem A and lolitrem B. Further, when pellets made from lolitrem neurotoxin-containing seed were fed to sheep, symptoms indistinguishable from those of severe, natural RGS were induced in four sheep, and mild symptoms in three sheep. 相似文献
8.
Abstract Extract Most perennial ryegrass (Lolium perenne) plants in pastures in New Zealand contain a naturally occurring fungal endophyte, Neotyphodium lolii. Endophyte-infected perennial ryegrass produces alkaloids that vary in concentration during the year. Some alkaloids enhance the persistence and productivity of ryegrass pastures by protecting them against insect attack. However, when other alkaloids are consumed they can reduce animal performance and lead to health problems. The alkaloid lolitrem B, for example, causes the neuromuscular disorder ryegrass staggers (Fletcher et al 1999). Clinical symptoms of ryegrass staggers in animals range from slight muscular tremors through to staggering and complete collapse. Severely affected animals create management problems and are prone to accidental death. Outbreaks occur sporadically, particularly in summer and autumn and affect sheep, cattle, deer, horses, llamas (Lama glama) and alpaca (Lama pacos). Lolitrem B appears to be a stable compound, which tends to be concentrated in the leaf sheath at the base of the ryegrass plant and in the seed-heads (di Menna et al 1992; Keogh et al 1996). Hay made from endophyte-infected perennial ryegrass contains large numbers of seed-heads and lolitrem B concentrations can be high, especially if it is made in late summer or autumn and, consequently, animals fed such hay are at risk of developing ryegrass staggers. 相似文献
9.
Fisher MJ Bohnert DW Ackerman CJ Schauer CS DelCurto T Craig AM Vanzant ES Harmon DL Schrick FN 《Journal of animal science》2004,82(7):2175-2184
Two experiments were conducted to evaluate perennial ryegrass straw as a forage source for ruminants. Experiment 1 evaluated digestion and physiological variables in steers offered perennial ryegrass straw containing increasing levels of ergot alkaloid, lolitrem B. Sixteen ruminally cannulated Angus x Hereford steers (231+/-2 kg BW) were blocked by weight and assigned randomly to one of four treatments. Steers were provided perennial ryegrass straw at 120% of the previous 5-d average intake. Before straw feeding, soybean meal was provided (0.1% BW; CP basis) to meet the estimated requirement for degradable intake protein. Low (L) and high (H) lolitrem B straws (<100 and 1,550 ppb, respectively; DM basis) were used to formulate treatment diets: 100% L; 67% L:33% H; 33% L:67% H; 100% H (DM basis). Intake and digestibility of DM and OM, and ruminal pH, total VFA, and NH3-N were not affected by increasing lolitrem B concentration. Ruminal indigestible ADF (IADF) fill increased linearly (P = 0.01) and IADF passage rate decreased linearly (P = 0.04) as lolitrem B increased. Experiment 2 evaluated performance and production by 72 Angus x Hereford cows (539+/-5 kg BW) consuming perennial ryegrass straw containing increasing lolitrem B during the last third of gestation. Cows were blocked by body condition score and randomly assigned to one of three treatments. Cows were provided perennial ryegrass straw ad libitum and supplemented with soybean meal (0.1% BW; CP basis) to meet the estimated requirement for degradable intake protein. Mixtures of a L and H lolitrem B straw (467 and 2,017 ppb, respectively) were used to formulate treatment diets: 100% L, 50% L:50% H, 100% H (DM basis). Thirteen of 24 cows on the 100% H treatment exhibited signs of ryegrass staggers and were removed from the study; nevertheless, lolitrem B concentration did not influence pre- or postcalving weight or body condition score change. These data suggest that feeding perennial ryegrass straw containing up to 1,550 ppb lolitrem B (DM basis) did not adversely affect nutrient digestion or physiological response variables in steers. However, providing straw with a lolitrem B concentration of approximately 2,000 ppb (DM basis) resulted in 54% of cows exhibiting signs of ryegrass staggers. These data suggest that blending straws with a high (>2,000 ppb) and low (<500 ppb) concentration of lolitrem B can be a successful management practice. 相似文献
10.
Miyazaki S Fukumura M Yoshioka M Yamanaka N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1013-1015
From 1997 to 1999, 29 cases of disorders were detected in cattle and horses that had been fed ryegrass straw imported from the U.S.A. These animals showed symptoms resembling ryegrass staggers and the clinical signs disappeared after removal of the straw. Endophytic hyphae were detected in the seeds of all straw samples that were responsible for the clinical cases. Lolitrem B concentrations in the straw ranged between 972 and 3740 ppb. Ergovaline concentrations were between 355 and 1300 ppb. Even though the concentrations of lolitrem B were lower than the toxic threshold proposed by Oregon State University in better part of the cases, our observations suggest the possibility that lolitrem B lower than the proposed threshold can bring disorders to sensitive individuals. 相似文献
11.
The varieties of perennial ryegrass (Lolium perenne) infected with the endophytic fungus Neotiphodium lolii contain several classes of toxic alkaloids, including ergopeptide alkaloids and lolitrem alkaloids. Lolitrem B, a potent tremorgen, is generally considered to be the predominant alkaloid in endophyte-infected perennial ryegrass. Ergovaline, a vasoconstrictor normally associated with endophyte-infected tall fescue (Festuca arudinacea), is also present in endophyte infected perennial ryegrass. Clinical signs of animals ingesting endophyte-infected perennial ryegrass are consistent with the presence of lolitrem B. However, clinical signs normally associated with ergovaline poisoning are not usually observed in animals ingesting endophyte-infected perennial ryegrass. A survey was conducted to quantitate both lolitrem B and ergovaline in 459 perennial ryegrass straw samples received at the Oregon State University College of Veterinary Medicine. Samples were analyzed for each alkaloid using separate high-performance liquid chromatography analyses. A strong positive correlation between the 2 alkaloids (r2 = 0.7335) was observed, especially in the samples containing <3,000 ppb (ng/g) lolitrem B. The threshold levels above which clinical signs typically occur are 2,000 ppb lolitrem B and 300-400 ppb ergovaline. All of the samples analyzed contained <425 ppb ergovaline. 相似文献
12.
Cristiane Vinhaes Gracindo Helder Louvandini Franklin Riet-Correa Marcos Barbosa-Ferreira Márcio Botelho de Castro 《Tropical animal health and production》2014,46(5):733-737
Brachiaria spp. are the most important grasses for ruminants in central-western Brazil. However, the use of these pastures is limited by their toxicity due to steroidal saponins. This experiment was conducted for 60 days to demonstrate the resistance of sheep raised on Brachiaria spp. pastures to steroidal saponin poisoning. The experiment was composed by 48 animals randomly divided into four groups (n?=?12). Among them, 32 4- to 5-month-old castrated male crossbred Santa Inês sheep, originating from flocks that had been grazing on Brachiaria spp. for more than three consecutive years, and 16 were non-adapted (naïve) sheep from flocks that never had prior contact with pastures of Brachiaria spp. were randomly divided into four groups. Each of the four experimental groups was composed by eight adapted and four non-adapted animals. The four experimental groups were introduced into paddocks, each of which contained a single grass: either Brachiaria decumbens, Brachiaria brizantha, Panicum maximum, or Andropogon gayanus. The addition of the naïve sheep to the groups was designed to detect pastures’ toxicity to naïve sheep and to adjust the stocking rate to optimize the use of forage. The weight gains of sheep grazing on B. decumbens, B. brizantha, and P. maximum were similar; however, the A. gayanus group showed lower weight gains compared with the other groups (P?<?0.05). The mean serum activities of γ-glutamyltransferase in the sheep grazing on B. decumbens were higher than those in the sheep from the other groups. No significant differences among the groups were found in aspartate aminotransferase, creatinine, albumin, or total protein serum concentrations. No clinical signs were observed in the adapted sheep in any of the pastures. Of the four non-adapted sheep introduced into the B. decumbens pasture, two showed clinical signs of steroidal saponin poisoning, and one died. No clinical signs were observed in the non-adapted sheep in the other pastures. The saponin (protodioscin) concentrations in the pastures varied from 3.3 to 12.2 g/kg DM in B. decumbens, from 2.8 to 9.1 g/kg DM in B. brizantha, and from 1 to 1.5 g/kg DM in A. gayanus. No saponins were found in P. maximum. It is concluded that sheep from flocks reared in pastures of B. decumbens and B. brizantha were resistant to steroidal saponin poisoning and showed similar weight gains to those of sheep grazing in other tropical pastures. 相似文献
13.
Abstract The cellulase dry matter disappearance (CDMD) and herbage nitrogen (N) of Lolium perenne (perennial ryegrass) were evaluated for pastures grown under subtropical conditions for two years, under five combinations of grazing frequency and intensity, each applied in a rotational grazing system. These quality factors were also evaluated for a simulated continuous grazing (CG) system. Low frequency, low intensity grazing produced lower CDMD and herbage N levels than higher grazing frequencies and intensities. These differences were, however, generally small. Overall, levels of herbage digestibility (estimated by CDMD) and N fell within similar ranges to those observed for perennial ryegrass in temperate environments. Seasonal trends in quality were characterized by high levels during late autumn to early summer (May to October) and low levels during mid‐summer to early autumn (January to April). These data would tend to support high frequency grazing as part of the management programme for perennial ryegrass. It is cautioned, however, that for subtropical conditions in South Africa, adopting frequent grazing may reduce persistence of perennial ryegrass. 相似文献
14.
AIM: To determine the effect of increasing molybdenum (Mo) intakes on serum and liver copper (Cu) concentrations and growth rates of grazing red deer (Cervus elaphus). METHODS: Molybdenum- and Cu-amended fertilisers were applied to six 1.1-ha paddocks in a 3 × 2 design. Three levels of Mo were applied on two paddocks at each level in mid April (designated Day 1); levels were: none (control), 0.5 (medium) and 1.0 (high) kg Mo/ha as sodium molybdate. In late May (Day 39), two levels of Cu (none and 3.0 kg Cu/ha, as copper sulphate) were applied to each of the three levels of Mo-treated paddocks. Pasture Mo, Cu and sulphur (S) concentrations were measured at about fortnightly intervals. In late June (Day 74), ten 6-month-old red deer hinds were placed on the six experimental pastures, and serum and liver Cu concentrations were monitored at about monthly intervals for 102 days. The hinds were weighed on four occasions during the trial. RESULTS: Mean pasture Mo concentrations on Day 56 were 2, 4.6 and 11.3 mg/kg dry matter (DM) for the untreated control, medium and high Mo-treated pastures, respectively. Pasture Cu concentration was 95 mg/kg DM on Day 59, 53 mg/kg DM on Day 90, and 9 mg/kg DM by Day 153. Mean S concentration in pasture was 3.3 (range 3.03–3.45) g/kg DM. Copper application to pasture had no significant effect on serum and liver Cu concentrations in deer so data were pooled within Mo treatment. Mean initial (Day 74) serum Cu concentration was 9.2 µmol/L. In the deer grazing the control Mo pasture, this increased to 10.3 µmol/L on Day 112, before decreasing to 6.4 µmol/L on Day 176. In deer grazing the medium and high Mo-treated pastures, mean serum Cu concentrations were 3.8 and 3.9 µmol/L, respectively, on Day 112, and 2.5 and 3.3 µmol/L, respectively, on Day 176. Mean initial (Day 74) liver Cu concentration was 131 µmol/kg fresh tissue. In the deer grazing the control Mo pasture, this declined to 120 and 52 µmol/kg on Days 112 and Day 176, respectively. In deer grazing the medium and high Motreated pastures, liver Cu concentrations decreased to 55 and 52 µmol/kg fresh tissue, respectively, on Day 112, and 21 and 20 µmol/kg fresh tissue, respectively, on Day 176. Mean serum and liver Cu concentrations were not significantly different between deer grazing the medium and high Mo-treated pastures, and were lower (serum p=0.003, liver p<0.001) in those groups than in deer grazing the untreated control pastures. No clinical signs of Cu deficiency associated with lameness were observed. Deer grazing pastures that had Mo concentrations >10 mg/kg DM had lower (p=0.002) growth rates (100 vs 130 g/day) than those on pastures containing <2.4 mg Mo/kg DM. CONCLUSION: Increasing pasture Mo concentrations from 2 mg/kg DM to ≥4.6 mg/kg DM significantly reduced serum and liver Cu concentrations in grazing deer. Reduced growth rate was observed at pasture Mo concentrations >10 mg/kg DM. 相似文献
15.
16.
Basri H. HAKYEMEZ Ahmet GOKKUS Turker SAVAS Ismail Y. YURTMAN 《Animal Science Journal》2009,80(1):34-40
This study aimed at identifying changes in natural pastures during the grazing season and investigating the effects of these changes on pasture feeding potential for high yielding dairy goats. During the study, 12 dairy goats were grazed on a 1.5 ha natural pasture for three months from April to June in 2003, 2004 and 2005. The goats were fed 0.5 kg/day of concentrate as a supplement during the grazing season. Botanical composition, herbage production and intake, crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of the pasture were determined. Live weight, milk yield, milk dry matter (DM) and fat content of the goats were monitored. The data were analyzed using a linear model, which evaluated the effects of grazing seasons in each year. Based on the three-year average, 87% of pasture was herbaceous plants and the remaining was shrubs in DM basis with Cistus creticus , Quercus ithaburensis , Pistacia atlantica and Asparagus acutifolius being the major shrub species. The herbage yield in June was significantly lower than in other months in all years ( P = 0.001). In all experimental years, the CP content of the pasture decreased but the structural carbohydrates increased as the grazing season proceeded. While live weight was not affected by grazing periods except for 2004 ( P = 0.001), milk yield significantly decreased with advancing grazing period ( P = 0.001). The results of the present study indicate that natural pasture has a supportive effect in April and May on the milk yield of lactating goats which are in mid-lactation, and suggested that supplementary feeding is required in consecutive grazing periods. 相似文献
17.
G. Steinheim Ø. A. Voie T. Ådnøy K. S. Longva Ø. Holand 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3):157-160
Abstract Ungulates grazing in military training areas may be exposed to non-combusted fragments of various explosives, among which 2-methyl-1,3,5-trinitrobenzene (TNT) and 1,3,5,7-tetranitro-1,3,5,7-tetrazocane (HMX) are two of the most common. This study investigated if sheep react to their pastures being contaminated with TNT or HMX. The experiment was conducted in fenced-in study areas on grass-dominated pasture. The eight sheep studied did not differentiate between clean pasture and contaminated pasture when choosing freely between plots containing TNT, HMX or no contamination. The results indicate that sheep grazing military training areas are unlikely to have behavioural responses reducing time spent grazing sites where TNT or HMX are present as fragments in the field layer. 相似文献
18.
Watson RH McCann MA Parish JA Hoveland CS Thompson FN Bouton JH 《Journal of animal science》2004,82(11):3388-3393
The nonergot alkaloid-producing endo-phyte, AR542, has been shown to improve the persistence and yield of tall fescue pastures without causing the animal disorders commonly associated with tall fescue toxicosis. A 3-yr grazing study was conducted to compare effects of AR542-infected tall fescue pastures with wild type endophyte-infected (E+) tall fescue pastures on cow-calf performance. Replicated 7.3-ha pastures of each treatment were grazed by cow-calf pairs (16 pairs per pasture replication) each year from March to weaning in September. The cows were exposed to breeding on their respective pasture treatments from April 1 through June 15. The treatment groups were compared for reproductive performance, ADG, BCS, calf growth rate, and weaning weight. Blood samples were also collected for serum prolactin (PRL) analysis. There were no significant differences in calving rate (P = 0.98) or calving interval (P = 0.62) between pasture treatments. Cows that grazed the AR542 pastures subsequently gave birth to calves that were heavier (P < 0.05) than calves from cows that had grazed the E+ pastures. Cows grazing the AR542 pastures had higher (P < 0.05) BCS at the end of the grazing period, and had higher ADG during the grazing period. Calves raised on the AR542 pasture had higher (P < 0.05) ADG and weaning weights than calves of the same sex raised on the E+ pastures. Serum PRL concentrations were decreased (P < 0.05) in both cows and calves on the E+ pastures compared with serum PRL concentrations in cows and calves grazing the AR542 pastures. The results indicate that grazing tall fescue pastures infected with the AR542 endophyte may give significant advantages in cow-calf growth rates and BCS over grazing E+ pastures. However, there did not seem to be any benefit in reproductive performance in this trial. There was a small, but significant increase in birth weight in cows grazing AR542 pasture. 相似文献
19.
将高寒山区白三叶／多年生黑麦草放牧草地植物组分分成白三叶、多年生黑麦草、单子叶杂草、阔叶杂草和死物质5种进行季节动态研究，进而对该草地牧草季节供给质量进行评价，找出生产管理上的不足并加以改进。该草地多年生黑麦草占据绝对优势，但豆科牧草含量不足；冬春季节死物质含量过高，夏秋季节利用强度不足。草地供给的牧草质量9月最高，播种牧草含量达79．90％，12月最差为44．68％；而可利用牧草含量最高为8月的97．04％，12月最低为45．55％。 相似文献
20.
Eric M. Mousel Walter H. Schacht Patrick E. Reece Amy E. Herron Ann Koehler 《Strength and Conditioning Journal》2011,64(2):208-214
Understanding the long-term effect of summer grazing date and fall stocking rate on herbage production is critical to extending the grazing season in the Nebraska Sandhills. A study was conducted from 1997 to 2002 at the Gudmundsen Sandhills Laboratory located near Whitman, Nebraska, to determine the herbage production response to summer grazing date and October stocking rate on two different sites. Site 1 was dominated by warm-season grasses and site 2 was dominated by cool-season graminoids. At each site, three 0.37-ha pastures were constructed in each of four blocks before application of summer grazing treatments. Pastures in each block were grazed at 0.5 animal-unit months (AUM) · ha?1 in June or July, or were deferred from summer grazing. Following summer grazing treatments, October stocking rate treatments (no grazing or 1.0, 2.0, or 3.0 AUM · ha?1) were applied to subunits of each summer grazing date pasture during mid-October. Vegetation was sampled in each pasture in mid-June and mid-August and sorted by functional group to determine the effect of 5 yr of grazing treatments on herbage production and residual herbage. Herbage production was not affected by summer or October grazing treatments on the warm-season grass–dominated site. Increasing October stocking rate, however, reduced cool-season graminoid production and subsequent herbage production 25% by year 5 of the study. Residual herbage at both sites at the end of the October grazing periods explained as much as 16% to 34% of subsequent year’s herbage production. Grazing managers in the Nebraska Sandhills can extend the grazing season by lightly stocking pastures in the summer to facilitate additional fall grazing. Heavy stocking in October over several years on cool-season–, but not warm-season–, dominated sites will reduce production of cool-season graminoids on these sites. 相似文献