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为了研究肌内脂肪与脂类合成代谢相关基因的关系,试验采用荧光定量PCR的方法检测了8头乌金猪和6头长白猪背最长肌脂肪酸合成酶(FAS)、固醇调节元件结合蛋白1c(SREBP-1c)和硬脂酰CoA去饱和酶(SCD)基因mRNA相对表达量,同时采用索氏提取法检测背最长肌肌内脂肪含量,并对两者进行相关性分析。结果表明:乌金猪FAS基因mRNA相对表达量极显著高于长白猪(P0.01);SCD基因mRNA相对表达量显著高于长白猪(P0.05);SREBP-1c基因mRNA相对表达量虽然高于长白猪,但差异不显著(P0.05)。乌金猪背最长肌肌内脂肪含量高于长白猪,且差异显著(P0.05)。乌金猪和长白猪的FAS和SCD基因mRNA相对表达量都与肌内脂肪含量呈极显著正相关(P0.01);乌金猪SREBP-1c基因mRNA相对表达量与肌内脂肪含量呈极显著正相关(P0.01),长白猪SREBP-1c基因mRNA相对表达量与肌内脂肪含量呈显著正相关(P0.05)。说明脂肪酸合成关键基因的mRNA相对表达量在乌金猪和长白猪间存在品种差异,这种差异可能是肌内脂肪含量不同的主要原因之一。 相似文献
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影响猪肉质性状遗传因素的研究进展 总被引：2，自引：1，他引：1
非遗传因素和遗传因素对猪肉质性状均有影响。前者包括饲养条件、转运、屠宰等众多因素。后者即遗传因素是影响猪肉品质的一个重要因素,主要包括控制肌内脂肪(intramuscular fat,IMF)沉积的主效基因MI基因,候选基因心脏脂肪酸结合蛋白(heart fatty acid binding protein,H FABP)基因和脂肪细胞型脂肪酸结合蛋白(A FABP)基因;MyoD基因家族是影响猪肉质性状的又一个遗传因素;此外还有氟烷基因、RN基因(rendement napole,RN)等。作者就影响猪肉品质的遗传因素作一综述。 相似文献
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家禽的肉质性状已经成为育种工作者一个重要的研究方向,而影响家禽肉质的因素有很多,如:遗传、营养、环境、疾病等。研究结果表明,肌内脂肪与肉的嫩度及肉质相关;另外,肌苷酸(IM P)是鸡肉质鲜味特性的主要物质基础,影响IM P生成的各种基因都是潜在的候选基因。作者综述了与肌内脂肪及肌苷酸合成代谢相关的脂蛋白脂酶(LPL)、细胞外脂肪酸结合蛋白(EX-FABP)、瘦蛋白受体(OBR)、解偶联蛋白(UCP)、腺苷琥珀酸裂解酶(ADSL)、腺苷一磷酸脱氨酶1(AM PD 1)等基因的研究进展。 相似文献
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《中国畜牧杂志》2019,(9)
本实验旨在探究布莱特黑牛脂肪型脂肪酸结合蛋白(A-FABP)基因表达规律,鉴定其与肌内脂肪含量调控的相关性。通过克隆获得A-FABP基因,采用生物信息学方法分析基因结构;利用荧光定量PCR技术研究A-FABP基因在不同组织及不同时期的表达情况;采用PCR-SSCP方法探究基因型与肌内脂肪含量的相关性。结果表明:克隆获得的A-FABP序列与牛、瘤牛、山羊亲缘关系近,同源性高;A-FABP基因在背最长肌中表达量最高,不同时期A-FABP基因的表达量随月龄增加逐渐降低;关联分析显示其多态位点与肌内脂肪含量显著相关(P<0.05),与大理石花纹评分不显著。综上推测,A-FABP基因参与牛肌内脂质代谢相关过程,对调控脂肪的沉积发挥重要作用,可作为肉质性状的辅助标记基因。 相似文献
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本试验旨在研究共培养体系中猪骨骼肌卫星细胞对肌内前体脂肪细胞脂质沉积的影响。试验分离培养了猪骨骼肌卫星细胞和肌内前体脂肪细胞,2种细胞分离培养鉴定后,接种于Transwell细胞小室共培养板进行共培养,待细胞密度达到90%以上分别进行诱导分化。骨骼肌卫星细胞诱导分化8 d后,检测肌内前体脂肪细胞分化水平、分化标志基因的表达以及脂质代谢关键酶的表达。结果显示:与肌内前体脂肪细胞单独培养相比,共培养体系内肌内前体脂肪细胞的脂滴数量和脂滴面积极显著减少(P0.01),肌内前体脂肪细胞增殖分化转录因子过氧化物酶体增殖物激活受体γ和CCAAT增强子结合蛋白的基因和蛋白表达水平极显著下降(P0.01),肌内前体脂肪细胞的乙酰辅酶A羧化酶、脂肪酸合成酶的基因和蛋白表达水平极显著下降(P0.01)。由此可见,共培养体系中猪骨骼肌卫星细胞对肌内前体脂肪细胞脂质沉积具有抑制作用。 相似文献
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《中国畜牧兽医》2020,(2)
肌内脂肪是影响肉品质的关键因素之一,影响着肉的嫩度、系水力、风味和多汁性。影响猪肌内脂肪沉积的因素较复杂,主要受品种、年龄、性别、营养、基因等多个因素影响。文章介绍了猪肌内脂肪的来源及其含量与猪肉嫩度、系水力、风味3个肉质性状的关联性,证实肌内脂肪与肉品质两者之间存在显著相关性;简述了影响猪肌内脂肪含量的几个主要因素,包括品种、体重、性别和日粮能量、蛋白质、维生素A等相关营养水平,脂肪酸合酶、心脏脂肪酸结合蛋白、脂联素等几个关键基因;初步阐释了这些因素对肌内脂肪沉积的影响及脂肪代谢机理,总结影响因素与肌内脂肪之间的规律;分析发现育种、候选基因和营养调控是改善肌内脂肪含量最有效的方法,将肉质更优的本土猪与外来猪进行杂交或对本土猪进行基因改良可能是未来较为有潜力的研究方向。 相似文献
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M. Árnyasi E. Grindflek A. Jávor & S. Lien 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(3):198-203
A highly significant quantitative trait locus (QTL) on pig chromosome 6, affecting intramuscular fat (IMF), has previously been detected by our group and others. Two genes of positional and biological interest, the small heterodimer partner (SHP; NR0B2) and the heart fatty acid binding protein (FABP3; H‐FABP), were investigated for meat quality traits and IMF respectively. SHP was partially sequenced (GenBank: DQ002896 and DQ002897 ) and mapped to the QTL region on porcine chromosome 6, affecting IMF. The map shows no recombination between SHP and FABP3, which was previously mapped to the same QTL region. Twelve single nucleotide polymorphisms were detected in the sequenced region of SHP gene. Haplotype information was used to investigate association between genetic variation and different meat quality traits. SHP haplotype combinations were found to have significant effect on connective tissue. However, further studies are needed to evaluate this possible association more effectively. The FABP3 is involved in fatty acid transport and has been studied as a candidate gene for IMF by several research groups. In our study, FABP3 genotypes were confirmed to be significantly associated with IMF in pigs. The average content of IMF in our population was 1.6%, which may indicate that the FABP3 polymorphism explains as much as 30–35% of the variation in IMF in our pig cross‐population. 相似文献
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C.R. Schwab B.E. Mote Z.-Q. Du R. Amoako T.J. Baas & M.F. Rothschild 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(3):228-236
Summary A sufficient level of intramuscular fat (IMF) is needed to enhance consumer acceptance of pork products, and is currently receiving greater attention within swine genetic improvement programmes. An examination of previously described and novel genetic variants within candidate genes for IMF deposition was performed to evaluate potential use of genetic markers in marker-assisted selection (MAS). Biological candidate genes implicated to play a role in adipogenesis were investigated within two different lines of purebred Duroc pigs. These included MC4R , FABP3 , DLK1 , and TCF7L2 . Significant variation in IMF within the control line was described by the MC4R genotype and a novel Bsr fI single nucleotide polymorphism within the FABP3 gene. Genetic markers for DLK1 and TCF7L2 evaluated in this population are not currently recommended for selection in Duroc swine. Existence of MC4R and FABP3 mutations may be useful markers in MAS aimed at IMF improvement, provided that gene effects are segregating and the presence of an association is detected within the population. However, additional work to confirm the use of the investigated genetic markers in selection programmes is needed. 相似文献
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Yong Wang Wenqiang Liu Chao Hang Yiqiang Du Ying Chen Jinming Xing Jun Gao Dexin Qiu 《Journal of animal physiology and animal nutrition》2019,103(5):1447-1452
This study aims to assess the association of polymorphisms and mRNA expression of adipocyte‐type fatty acid‐binding protein (A‐FABP) with intramuscular fat (IMF) in the breast muscle (BM) and leg muscle (LM) of Baicheng‐You chickens (BYCs). A total of 180 chickens, including sixty black Baicheng‐You chickens (BBYCs), sixty silky Baicheng‐You chickens (SBYCs) and sixty white Baicheng‐You chickens (WBYCs), were reared from 1 to 120 day. A polymerase chain reaction–single‐strand conformation polymorphism strategy (PCR‐SSCP) was used to detect the polymorphism of the A‐FABP gene in the first exon, and the C51T silent mutational site was found. The IMF content with the AA genotype was significantly higher than that with the AG genotype (p = 0.0473) in the LM of WBYC. Thus, this site could be taken as a molecular marker in selecting a higher IMF content of LM in WBYC. A‐FABP gene mRNA expression in the BM and LM of BYCs was detected, and a significant positive correlation was observed in the LM of WBYC. These findings provide fundamental data that might be useful in further study of the role of the A‐FABP gene in IMF content and fatty metabolism in chickens. 相似文献
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Serão NV Veroneze R Ribeiro AM Verardo LL Braccini Neto J Gasparino E Campos CF Lopes PS Guimarães SE 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2011,128(1):28-34
Seventy-two pigs of three genetic groups (Brazilian indigenous breed Piau, Commercial line and Crossbred) of both sexes were slaughtered at four live weights (30, 60, 90 and 120 kg). Intramuscular fat (IMF) content in Longissimus dorsi muscle of each animal was extracted and correlated with candidate gene mRNA expression (ATN1, EEF1A2, FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1). Within slaughter weight of 120 kg, Piau and Crossbred pigs showed higher IMF content (p < 0.05) than commercial animals, with 2.48, 2.08 and 1.00% respectively. Barrows presented higher values of IMF (p < 0.05) than gilts (1.54 and 1.30% respectively). Gene expression of EEF1A2, FABP3, LDLR, OBSCN, PDHB, TRDN and RYR1 were correlated with IMF (p < 0.05) using the whole dataset. For Piau data only, expression of FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1 showed correlation with IMF (p < 0.05). Genes that have important roles in lipid transportation inside the cell (FABP3) and tissues (LDLR) showed correlation with IMF of, respectively, 0.68 and 0.63 using the whole data set, and 0.90 and 0.91 using data from Piau animals. The highly positive correlation of the LDLR and FAPB3 expression with IMF content may confirm that these genes are important for fat deposition in the porcine L. dorsi muscle. 相似文献
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The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs 总被引：46，自引：0，他引：46
Gerbens F de Koning DJ Harders FL Meuwissen TH Janss LL Groenen MA Veerkamp JH Van Arendonk JA te Pas MF 《Journal of animal science》2000,78(3):552-559
Effects of genetic variation in porcine adipocyte and heart fatty acid-binding protein genes, A-FABP and H-FABP, respectively, on intramuscular fat (IMF) content and backfat thickness (BFT) were examined in F2 crossbreds of Meishan and Western pigs. The involvement of each FABP gene in IMF accretion was studied to confirm previous results for Duroc pigs. The F2 crossbred pigs were genotyped for various markers including microsatellite sequences situated within both FABP genes. Linkage analysis assigned the A-FABP and H-FABP genes to marker intervals S0001-S0217 (20 cM) on SSC4 and Sw316-S0003 (16.6 cM) on SSC6, respectively, refining previous chromosomal assignments. Next, the role of both chromosome regions/genes on genetic variation in IMF content and BFT was studied by 1) screening SSC4 and SSC6 for QTL affecting both traits by performing a line-cross analysis and 2) estimation of the effect of individual A-FABP and H-FABP alleles on both traits. In the first analysis, suggestive and chromosome-wise significant evidence for a QTL affecting IMF was detected on SSC6. The H-FABP gene is a candidate gene for this effect because it resides within the large region containing this putative QTL. The second analysis showed a considerable but nonsignificant effect of H-FABP microsatellite alleles on IMF content. Suggestive evidence for a QTL affecting BFT was found on SSC6, but H-FABP was excluded as a candidate gene. In conclusion, present and previous results support involvement of H-FABP gene polymorphisms in IMF accretion independently from BFT in pigs. Therefore, implementation of these polymorphisms in marker-assisted selection to control IMF content independently from BFT may be considered. In contrast to previous findings for Duroc pigs, no evidence was found for an effect of the A-FABP gene on IMF or BFT in this population. 相似文献
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为研究营养水平对杂交肉牛心脏型脂肪酸结合蛋白（FABP3）基因表达及肌内脂肪含量（IMF）的影响,以西门塔尔、利木赞和夏洛莱杂交肉牛为研究对象,利用实时荧光定量PCR技术,分别测定高、中、低营养水平的3种杂交肉牛背部脂肪中FABP3mRNA表达水平,并测定其背最长肌IMF。结果：（1）高营养组中夏洛莱牛IMF高于同营养组其他2种肉牛,其背部脂肪中FABP3mRNA水平也显著高于另外2种杂交肉牛（P〈0.01）;中营养组中西门塔尔和利木赞牛IMF均高于同营养组的夏洛莱牛,但FABP3mRNA的水平无显著差异（P〉0.05）;低营养组中夏洛莱牛IMF和FABP3mRNA水平均显著低于另外2种肉牛（P〈0.01）;（2）夏洛莱杂交肉牛背部脂肪中FABP3mRNA水平与IMF具有显著的相关性（P〈0.05）,而西门塔尔和利木赞牛中FABP3mRNA水平与IMF无显著相关性（P〉0.05）。该结果表明提高营养水平影响FABP3mRNA表达并在一定程度上影响IMF,但影响显著与否存在品种差异,在肉牛育种和牛肉生产中应当同时考虑营养因素和遗传因素。 相似文献
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Hai‐bo Zhang Zhi Sheng Wang Quan‐Hui Peng Cui Tan Hua‐Wei Zou 《Animal Science Journal》2014,85(4):411-419
This study was conducted to estimate different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks. Results showed that supplementary dietary protein significantly increased final weight, average daily gain (ADG), intramuscular fat (IMF), serum free fatty acid (FFA), total triglycerides, total cholesterol (Ch), low‐density lipoprotein cholesterol (LDL) and high‐density lipoprotein cholesterol (HDL) content. There was a quadratic response of ADG, IMF, FFA, Ch, HDL and LDL to dietary crude protein (CP) level. Lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl‐CoA carboxylase (ACC) enzyme activities were significantly increased by supplementary dietary CP, while hormone‐sensitive lipase (HSL) and carnitine palmitoyltransferase‐1 (CPT‐1) activities were significantly decreased. LPL, ACC and FAS enzyme activities showed quadratic increase as dietary CP increased. Peroxisome proliferator‐activated receptor γ (PPARγ), LPL, FAS, sterol regulatory element binding protein 1 (SREBP‐1), ACC, stearoyl‐CoA desaturase (SCD) and heart fatty‐acid binding protein (H‐FABP) gene expression were significantly increased by supplementary dietary CP, while HSL and CPT‐1 gene expression were significantly decreased. PPARγ, LPL, SREBP‐1, ACC and H‐FABP gene expression showed quadratic increase as dietary CP increased. These results indicated that supplementary dietary protein increased IMF accumulation mainly to increased intramuscular lipogenic gene expression and decreased lipolytic gene expression. 相似文献
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Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. 总被引：37，自引：0，他引：37
F Gerbens A J van Erp F L Harders F J Verburg T H Meuwissen J H Veerkamp M F te Pas 《Journal of animal science》1999,77(4):846-852
In order to find genetic markers to improve the meat quality of pigs by breeding we studied the relationship between variation in the heart fatty acid-binding protein (H-FABP) gene (FABP3) and intramuscular fat (IMF) content. To estimate the effect of H-FABP, pigs from two Duroc populations were selectively mated in such a way that at least two genotypes were present in each litter. In total, data from 983 pigs and pedigree information from three preceding generations were analyzed. Offspring were tested for IMF content as well as backfat thickness (BFT), BW, and drip loss of the meat (DRIP). All pigs were assigned to H-FABP RFLP genotype classes either by the assessed genotype (75%) or based on a probability score determined according to genotypic information of their relatives (25%). Contrasts were detected between homozygous H-FABP RFLP genotype classes for IMF content (.4%, P < .05), BFT (.6 mm, P < .01), and BW (2.4 kg, P < .10). No significant contrasts were detected for DRIP. Results for IMF content, BFT, and BW were confirmed when only genotyped animals were analyzed. Variation in BFT partially explained the effect on IMF content. Although other closely linked genes on porcine chromosome 6 might be responsible for the observed effect, interference of the halothane gene was excluded because all parental animals were noncarriers. In conclusion, H-FABP RFLP can be used as markers to select for increased IMF content and growth in breeding programs. 相似文献
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Dietary conjugated linoleic acids increase intramuscular fat deposition and decrease subcutaneous fat deposition in Yellow Breed × Simmental cattle 
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下载免费PDF全文 Haibo Zhang Xianwen Dong Zhisheng Wang Aiming Zhou Quanhui Peng Huawei Zou Bai Xue Lizhi Wang 《Animal Science Journal》2016,87(4):517-524
This study was conducted to estimate the effect of dietary conjugated linoleic acids (CLA) on intramuscular and subcutaneous fat deposition in Yellow Breed × Simmental cattle. The experiment was conducted for 60 days. The results showed that the average backfat thickness, (testicles + kidney + pelvic) fat percentage and subcutaneous fat percentage in dietary CLA were significantly lower than in the control group, while intramuscular the fat percentage was significantly higher. Compared to the control group, the Longissimus muscle enzyme activities of lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl‐coenzyme A carboxylase (ACC) in dietary CLA and the subcutaneous fat enzyme activities of LPL, hormone‐sensitive lipase (HSL) and carnitine palmitoyltransferase‐1 (CPT‐1) were significantly increased. Similarly, compared to the control group, the Longissimus muscle sterol regulatory element binding protein 1 (SREBP‐1), FAS, stearoyl‐coenzyme A desaturase (SCD), ACC, peroxisome proliferator‐activated receptor γ (PPARγ), heart fatty‐acid binding protein (H‐FABP) and LPL gene expression in dietary CLA were significant increased, as were the subcutaneous fat of PPARγ, H‐FABP, LPL, CPT‐1 and HSL in dietary CLA. These results indicated that dietary CLA increases IMF deposition mainly by the up‐regulation of lipogenic gene expression, while decreasing subcutaneous fat deposition mainly by the up‐regulation of lipolytic gene expression. 相似文献
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《中国畜牧杂志》2017,(3)
研究采用PCR-RFLP技术分析苏姜猪、姜曲海猪、长白猪群体A-FABP基因遗传多态性,并用统计学方法分析基因座多态与肉质性状的相关性。结果表明:3个猪种A-FABP基因内含子1均具有Bsm I酶切多态性,分别检测到3种基因型(AA、AB和BB),χ~2适合性检验显示,3个猪种A-FABP基因型分布处于Hardy-Weinberg平衡(P0.05)。多态信息含量(PIC)分析显示,3个品种猪均为中度多态;基因型与肉质性状关联分析显示,不同基因型肌内脂肪含量、大理石纹差异显著,大理石纹AA、AB型显著高于BB型(P0.05),肌内脂肪含量AA显著高于AB、BB型(P0.05)。结论显示,苏姜猪、姜曲海猪、长白猪A-FABP基因intron 1多态性对肉质性状影响显著。 相似文献