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1.
Diagnosis of duck plague in waterfowl by polymerase chain reaction 总被引：19，自引：0，他引：19
A recently developed polymerase chain reaction (PCR) assay was used for diagnosis of duck plague in waterfowl tissues from past and current cases of waterfowl mortality and to identify duck plague virus in combined cloacal/oral-pharyngeal swab samples from healthy mallards (Anas platyrhynchos) after a disease outbreak. The PCR was able to detect viral DNA from all the individual or pooled tissues assayed from 10 waterfowl, including liver and spleen samples from three Muscovy ducks (Cairina moschata domesticus) that did not yield virus isolates. The strong staining intensity of the PCR products from the waterfowl tissues indicated that large amounts of virus were present, even when virus was not isolated. Duck plague DNA was also detected in a cloacal swab sample from a wood duck (Aix sponsa) carcass submitted for diagnosis. The PCR assay identified duck plague DNA in 13 swab samples that produced virus isolates from carrier mallards sampled in 1981 after a duck plague die-off. The duck plague PCR clearly demonstrated the ability to quickly diagnose duck plague in suspect mortality cases and to detect virus shed by carrier waterfowl. 相似文献
2.
Chlamydophila psittaci (C. psittaci) infection was evaluated in 77 free-living nestlings of Blue-fronted Amazon parrots (Amazona aestiva) and Hyacinth macaws (Anodorhynchus hyacinthinus) in the Pantanal of Mato Grosso do Sul, Brazil. Tracheal and cloacal swab samples from 32 wild parrot and 45 macaw nestlings were submitted to semi-nested PCR, while serum samples were submitted to complement fixation test (CFT). Although all 32 Amazon parrot serum samples were negative by CFT, cloacal swabs from two birds were positive for Chlamydophila DNA by semi-nested PCR (6.3%); these positive birds were 32 and 45 days old. In macaws, tracheal and cloacal swabs were positive in 8.9% and 26.7% of the samples, respectively. Complement-fixing antibodies were detected in 4.8% of the macaw nestlings; macaw nestlings with positive findings were between 33 and 88 days old. These results indicate widespread dissemination of this pathogen in the two evaluated psittacine populations. No birds had clinical signs suggestive of chlamydiosis. To the best of our knowledge, this is the first report on C. psittaci in free-living Blue-fronted Amazon parrots and Hyacinth macaws in Brazil. 相似文献
3.
2011年4月份,河北省某地一些鸭场饲养的20～45日龄的樱桃谷肉鸭发生了以腹腔中充满大量清亮、茶色或啤酒样腹水为主要病变特征的疾病,即鸭腹水综合征。采集两个不同日龄的病死鸭肝脏样品,接种SPF鸭胚进行病毒分离,获得两个病毒分离物（HB01和HB02）。这两个病毒分离物对鸡、鸭和鹅的红细胞均无血凝活性。RT-PCR或PCR检测表明,HB01和HB02中鸭肝炎病毒检测为阳性,而鸭呼肠孤病毒、鸭圆环病毒、鸭黄病毒等检测均为阴性。将HB02接种5日龄SPF鸭,致死率为40%;对20日龄的鸭不致死。HB02分离物中鸭肝炎病毒的序列分析表明其与鸭肝炎病毒NA株及弱毒疫苗株C80和F64遗传距离较近。根据试验结果,推测鸭肝炎病毒可能是诱发本次鸭腹水综合症的因素之一。 相似文献
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5.
旨在调查和分析广东省养禽场肠球菌的亚型屎肠球菌和粪肠球菌耐药性及其毒力因子流行分布特征,为控制禽源肠球菌耐药性传播、保障公共卫生安全提供理论依据。作者于2018年从广东省4个养禽场采集肠道样品493份,进行屎肠球菌和粪肠球菌的分离鉴定;采用琼脂二倍稀释法测定肠球菌的最小抑菌浓度（MIC）;PCR方法检测肠球菌的耐药基因和毒力基因。结果显示：1）共分离到125株肠球菌,其中粪肠球菌84株（鸡源66株,鸭源18株）;屎肠球菌41株,均来自鸡肠道样本。2）菌株对四环素、多西环素、红霉素几乎全部耐药,对氟苯尼考和氯霉素的耐药率高达89.60%和74.40%。屎肠球菌耐药率普遍高于粪肠球菌,而粪肠球菌对环丙沙星和利奈唑胺的耐药率高于屎肠球菌;鸭源粪肠球菌对利奈唑胺的耐药率（94%）显著高于鸡源粪肠球菌（39.4%）,屎肠球菌对利奈唑胺均敏感。从鸡分离的1株粪肠球菌对万古霉素耐药。3）耐药基因在屎肠球菌中的检出率高于粪肠球菌,鸭源分离株检出率高于鸡源。耐药基因tetL、fexA、ermB最为流行,检出率均高于90%。其次是optrA基因,检出率为73.60%,poxtA和fexB的检出率均低于20%。在3株鸭源粪肠球菌中检测出cfr基因。4）已检测的毒力基因中efaA的携带率最高,为63.04%（58/92）,其他依次为gelE（54.35%,50/92）、ace（47.83%,44/92）、asa1（44.57%,41/92）。对环丙沙星及高浓度氨基糖苷类耐药的菌株及携带cfr基因的菌株,大多携带agg、asal、gelE或ace。本研究显示养殖场禽源肠球菌耐药严重,鸭源肠球菌对利奈唑胺耐药率高,耐药基因和毒力基因流行且多样,且检测出人医临床重要抗生素耐药基因,应加强对养禽场肠球菌耐药性监测。 相似文献
6.
Forty-nine avian chlamydial strains, isolated mainly from various regions in France and from different species of birds, were analyzed and tested with a panel of nine monoclonal antibodies (MAbs) by the indirect microimmunofluorescence test (MIF). The MAbs included five serovar-specific MAbs, three MAbs raised against Chlamydia psittaci and Chlamydia pecorum ovine strains, and one genus-specific MAb. Of the 49 isolates, 41 came from parrots or budgerigars; the rest were from pigeons, a canary, a duck, and a dove. Two additional strains were from unknown hosts. Most of these avian strains were successfully serotyped according to their reactions with five serovar-specific MAbs by the MIF test. The serovars of 44 strains were determined: 39 were of serovar A, 3 of serovar B, and 2 of serovar E. The remaining five isolates were unclassified because they did not react with any of five serovar-specific MAbs but did react with genus MAb or the MAbs produced with ovine strains. The five unclassified isolates (two from budgerigars, two from Gabon gray parrots, and one from a duck) indicate that one or more additional serovars of C. psittaci exist in birds. The heterogeneity within each subgroup was evident because the 49 avian isolates gave 10 subgroups when the results of the five serovar-specific MAbs were combined with results from the three MAbs produced with ovine strains. This heterogeneity of the serovar isolates, as shown by the combination of MAbs, could provide strain markers very useful for epidemiologic studies. 相似文献
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In a previous study we described the use of a new set of PCR primers (CpsiA/CpsiB) specific of the conserved pmp-family genes of Chlamydophila abortus as an efficient tool for the detection of these bacteria in ruminants including also preliminary results on avian strains. In this work, the use of this set of primers was extended to representative strains of the six major avian serovars (serovars A-F) and to field isolates of C. psittaci. For all the studied representative strains, using purified genomic DNA as a template, CpsiA/CpsiB primers allowed, as observed for C. abortus, a minimal 10-fold PCR signal increase compared to the one observed with ompA specific primers. In comparison to primers targeting the 16S-23S rRNA intergenic spacer, similar or increased sensitivity was observed depending on the strain. All the field isolates were amplified with CpsiA/CpsiB primers. On clinical samples, our primers are the best among those tested for detection of C. psittaci by simple conventional PCR. RFLP experiments performed using PCR fragments amplified with the CpsiA/CpsiB primers gave promising results demonstrating that these primers may provide an interesting tool for molecular typing when the bacterium cannot be grown from pathological samples. 相似文献
8.
Van Loock M Geens T De Smit L Nauwynck H Van Empel P Naylor C Hafez HM Goddeeris BM Vanrompay D 《Veterinary microbiology》2005,107(1-2):91-101
Two hundred turkey sera from eight Belgian and two French farms were tested for the presence of antibodies against avian pneumovirus (APV), Ornithobacterium rhinotracheale (ORT), Mycoplasma gallisepticum, Mycoplasma meleagridis and Chlamydophila psittaci. At slaughter, C. psittaci, APV and ORT antibodies were detected in 94, 34 and 6.5% of the turkeys, respectively. No antibodies against M. gallisepticum or M. meleagridis were present. Additionally, turkeys on three Belgian farms were examined from production onset until slaughter using both serology and antigen or gene detection. All farms experienced two C. psittaci infection waves, at 3-6 and 8-12 weeks of age. Each first infection wave was closely followed by an ORT infection starting at the age of 6-8 weeks, which was still detectable when the second C. psittaci infection waves started. Animals on farm A were not vaccinated against APV leading to an APV subtype B outbreak accompanying the first C. psittaci infection wave. Despite subtype A APV vaccination on farms B and C, the second C. psittaci infection waves were accompanied (farm B) or followed (farm C) by a subtype B APV infection. On all farms respiratory signs always appeared together with a proven C. psittaci, APV and/or ORT infection. This study suggests an association between C. psittaci, APV and ORT, and indicates the multi-factorial aetiology of respiratory infections in commercial turkeys. All three pathogens should be considered when developing prevention strategies for respiratory disease. 相似文献
9.
【目的】为防控鸭源沙门菌感染,本试验针对四川德阳地区鸭源沙门菌的感染情况开展研究。【方法】从该地区3个种鸭场和1个鸭孵化场共采集不同类型样本222份,按国标GB 4789.4－2016方法对沙门菌进行分离鉴定,进一步通过K-B法检测分离菌对14种抗菌药物的敏感性,并对鉴定出的稀有血清型沙门菌进行致病性研究。【结果】疑似沙门菌分离株在BS琼脂上呈黑色、灰色或棕褐色、有金属光泽菌落,在XLD琼脂上呈无色透明、或中心黑色或几乎全黑的菌落。将疑似沙门菌株接种三糖铁琼脂斜面进行生化鉴定,疑似沙门菌分离株均能使三糖铁斜面变为红色,底部变为黄色带黑色,符合沙门菌生化特性。通过PCR对沙门菌特异性基因invA进行扩增,经琼脂糖凝胶电泳后可在500 bp左右观察到目的条带,确认共分离到17株沙门菌,总分离率为7.7%（17/222）,包括鼠伤寒沙门菌、哈托沙门菌和波恩沙门菌3种血清型,其比例分别为47.1%（8/17）、29.4%（5/17）和23.5%（4/17）,优势血清型为鼠伤寒沙门菌。分离菌对β-内酰胺类和氨基糖苷类抗菌药耐药率最高,对氨苄西林和链霉素的耐药率分别为82.4%（14/17）和88.2%（15/17）,对喹诺酮类抗菌药最敏感,其中对萘啶酸100%（17/17）敏感。分离菌存在严重的多重耐药现象,其中耐10种以上（包括10种）抗菌药的6株,占比35.2%,且10种抗菌药分别来自至少6类不同种类抗菌药;耐8种抗菌药物的2株,占比11.8%;耐5~7种抗菌药的6株,占比35.3%;耐2~3种抗菌药物的共3株,占比17.6%。通过寇氏改良法测得稀有血清型菌株H4、B2的LD50分别为3.98×106和1.58×106 CFU,致病性试验结果表明,哈托和波恩沙门菌均能引起小鼠急性死亡,脏器充血、出血,细胞变性等。【结论】本研究成功分离到17株鸭源沙门菌,从鸭中分离到哈托沙门菌在国内外属首次。分离菌具有严重的耐药性和多重耐药现象,2株稀有血清型沙门菌对小鼠的致病力均较强,且致病作用相似。本研究结果可为鸭场沙门菌病的防治提供参考。 相似文献
10.
Kite VG Boyle DB Heine HG Pritchard I Garner MG East IJ 《Australian veterinary journal》2007,85(6):236-242
OBJECTIVE: To determine the prevalence and distribution of antibodies to Newcastle disease virus on Australian chicken farms and to determine the pathotype and relationships of the Newcastle disease viruses present on those farms. DESIGN: A cross-sectional survey of 753 commercial chicken farms. PROCEDURE: The survey comprised a detailed questionnaire and collection of venous blood samples. The titre of antibodies to Newcastle disease virus was determined by haemagglutination inhibition. Virus isolation was conducted from cloacal and tracheal swabs taken from chickens in serologically positive flocks. Virus isolates were pathotyped on the basis of the deduced Fusion protein cleavage site determined by nucleotide sequencing of a 265 bp region of the genome in the region of the cleavage site. RESULTS: Antibody evidence of Newcastle disease virus infection was found on 300 of the 753 surveyed farms throughout all 11 geographic regions of the survey. The highest prevalence occurred in the Sydney basin, New South Wales and Victoria east regions. Antibody titres were also highest in the regions where serologically positive flocks were most prevalent. The 259 virus isolates revealed nine different RNA sequences. Of the nine virus groups isolated, the most common group W was identical in sequence to the V4 vaccine strain. Five of the other groups had novel RNA sequences in the region of the F protein cleavage site. CONCLUSIONS: Antibodies to Newcastle disease virus are highly prevalent in the Australian chicken flock but all identified strains were avirulent in nature. 相似文献
11.
山羊支原体性肺炎流行病学调查 总被引：2，自引：1，他引：1
山羊支原体性肺炎是威胁山羊养殖的重要传染病,为了解其流行情况,对四川省主要山羊养殖地区的山羊支原体性肺炎进行了流行病学调查。从四川省7个地区山羊养殖场采集肺脏和鼻腔棉拭子样本共135份,经过分离鉴定得到42株支原体,其中绵羊肺炎支原体36株,丝状支原体6株;其中6个羊场仅分离到绵羊肺炎支原体,1个羊场同时分离到绵羊肺炎支原体和丝状支原体。本试验结果表明,绵羊肺炎支原体是引起四川省山羊支原体性肺炎的主要病原,个别地方存在绵羊肺炎支原体和丝状支原体混合感染。 相似文献
12.
为了解广西玉林市2020年规模禽场禽流感病毒感染状况,采用荧光RT-PCR方法,对广西玉林市7个县(市、区)42个规模化禽场采集的1260份禽喉/泄殖腔棉拭子样品进行了通用型禽流感病毒核酸检测(荧光PCR),并对检测为阳性的样本进行H5、H7亚型(双重荧光PCR)和H9亚型(荧光PCR)分型鉴定。结果显示:在42个规模化禽场中,未检出H5和H7亚型高致病性禽流感病毒阳性样品;在2个鸡场中检出18份H9亚型低致病性禽流感病毒阳性样品,在2个鸡场和4个鸭场中检出115份其他亚型低致病性禽流感病毒阳性样品。结果表明:在高致病性禽流感(H5+H7)三价灭活疫苗强制免疫政策下,广西玉林市规模化禽场的高致病性禽流感病毒感染风险较小,但仍须加强禽流感的免疫、监测,做好综合防控,以降低禽流感病毒由低致病性重组变异为高致病性的风险。本检测为指导广西玉林市禽流感防控提供了依据。 相似文献
13.
从贵州省临床疑似鸭传染性浆膜炎发病鸭鹅中分离病原菌,经细菌分离培养、细菌形态、培养特征及生化试验,初步鉴定出疑似鸭疫里默氏杆菌(Riemerella anatipestifer,RA)20株,经PCR和动物接种试验,其中9株鉴定为RA。对不同地区分离到的这9株菌进行血清型鉴定和15种常规抗菌药物的药敏试验。结果发现,9株RA中4株为血清2型,其余5株暂未鉴定出血清型;9株分离株中,对吡哌酸、链霉素、卡那霉素和红霉素等耐药的菌株比例为80%以上,对先锋霉素高度敏感的菌株比例较高。结果表明,本次分离的RA菌株生化试验鉴定结果与其他地区存在一定差异,不同地区分离到的RA对不同抗菌药的敏感程度存在较大差异性。本研究结果为该地区鸭传染性浆膜炎的药物防制提供了很好的理论依据。 相似文献
14.
Parisi A Lanzilotta SG Addante N Normanno G Di Modugno G Dambrosio A Montagna CO 《Veterinary research communications》2007,31(1):113-123
Eleven cattle farms, 8 layer farms, 7 broiler farms and 30 broiler meat samples were investigated in south-eastern Italy throughout
2003 to evaluate the prevalence, the molecular type and antimicrobial resistance of thermophilic Campylobacters. A total of
398 samples were analysed. One Campylobacter isolate for each positive faecal swab and three isolates per positive broiler meat sample were selected for further analysis.
Multiplex PCR was performed for species-level identification and PCR-RFLP of the flagellin A gene for genotyping. Resistance
to 14 antimicrobials was studied in 188 Campylobacter isolates. Prevalence of campylobacters was high both on farms (100%) and in food samples (73%). On 4/11 cattle farms and
on 10/15 poultry farms more than one species was isolated. The presence of more than one genotype was found on 8/11 cattle
farms, on 10/15 poultry farms and in 8/22 Campylobacter-positive food samples. High rates of resistance to quinolone were observed: 9/31 (29%) C. jejuni bovine isolates, 4/22 (18%) C. jejuni poultry isolates, and 14/26 (54%) C. coli poultry isolates. Resistance to sulphamethoxazole-trimethoprim was also observed frequently: 18/26 (69%) of the avian C. coli strains, 25/31 (80%) of the C. jejuni strains isolated from poultry and 15/22 (68%) of those isolated from cattle were resistant. There was a significant difference
between the rate of resistance to macrolides of C. coli and C. jejuni isolated in poultry, which amounted to 23% and 3%, respectively. This study provided data on the prevalence and antimicrobial
resistance of thermophilic campylobacters in south-eastern Italy and confirmed that flaA-typing is an efficient tool to study the epidemiology of Campylobacter strains in short-term investigations. 相似文献
15.
Pasmans F Martel A Boyen F Vandekerchove D Wybo I Immerseel FV Heyndrickx M Collard JM Ducatelle R Haesebrouck F 《Veterinary microbiology》2005,110(3-4):285-291
Reptile-associated salmonellosis in humans is an increasing public health issue. This study aimed at characterizing Salmonella isolates from captive lizards and to compare them to human isolates. Salmonella was isolated from 25 of 33 cloacal and 47 of 79 faecal samples from captive lizards (75.8 and 59.5%, respectively). The strains belonged to 44 serotypes of subspecies I (27 serotypes), II (9), IIIb (3) and IV (5). Two strains, one of serotype Enteritidis and one of serotype Amsterdam, were resistant to nitrofurantoin. Invasion assays in Caco-2 cells were performed with 40 saurian isolates of subspecies I, 15 isolates of subspecies II, 4 strains of subspecies IIIb, 6 subspecies IV isolates and 17 human isolates of corresponding serotypes of subspecies I. Saurian isolates belonging to subspecies I invaded the Caco-2 cells to a higher extent than those from the other subspecies. The human isolates invaded the Caco-2 cells to a lesser degree compared to their saurian counterparts. In the same strains, the presence of virulence genes agfA, shdA, spvR, pefA and sopE was determined using PCR. Whereas agfA was detected in all strains, pefA was only detected in one saurian and in the human serotype Enteritidis strains. The spvR gene was detected in the same serotype Enteritidis strains and in 33% of the subspecies IV strains. The shdA gene was present in all the human isolates and in 86% of subspecies I saurian isolates. SopE was found in 17% of the human isolates, in 24% of the saurian subspecies I strains and in all of the subspecies IV strains. 相似文献
16.
P M Gethings G L Stephens J M Wills P Howard A H Balfour A I Wright K L Morgan 《The Veterinary record》1987,121(10):213-216
The prevalence of infection with Chlamydia psittaci, Toxoplasma gondii, Toxocara cati and Microsporum canis was examined in 51 cats on 22 sheep farms in the Bristol area. Serum antibody to C psittaci and T gondii was present in 45 per cent and 47 per cent of cats, respectively. At the time of sampling C psittaci was isolated from 6 per cent of the cats, T cati was identified in 63 per cent of faecal samples but neither T gondii nor M canis was isolated. When examined according to the farm of origin, 22.7 per cent of farms had cat populations with no evidence of infection with C psittaci or T gondii. Of the remainder, 45.5 per cent supported cat populations with evidence of both infections and 31.8 per cent had evidence of T gondii infection alone. None of the farms had cat populations with evidence of C psittaci infection alone. Two of the cats infected with C psittaci were excreting viable organisms in the faeces. The possible significance of this to the epidemiology of ovine enzootic abortion is discussed. 相似文献
17.
为了解鸡滑液囊支原体(MS)在川西地区的感染情况,本研究采集15个肉鸡场疑似MS感染的病鸡咽拭子、跗关节和胸部滑液囊样本共75份,对经PCR检测为阳性的样本进行MS分离,并对分离株的主要生物学特性进行研究,以及对分离株的VlhA基因进行遗传进化分析。结果显示,75份样本MS PCR阳性检出率为41.33%(31/75),11个鸡场感染该病,场阳性率为73.33%(11/15);但仅从其中3个鸡场分离到9株MS,分离株菌落与菌体形态与已知MS培养特性相符,各分离株培养浓度为10~4CCU/mL^10~6CCU/mL,分离株以5×10~5CCU接种7日龄SPF鸡胚,鸡胚于接种后7 d^9 d死亡,且分离回收到接种菌株;9株MS分离株VlhA基因的同源性为86.1%~99.9%,与参考株同源性为86.2%~95.4%,其中分离自同一鸡场的7株MS VlhA基因同源性为96.4%~99.9%;分离株VlhA基因遗传进化分析显示,其中分离自两个不同鸡场的两株MS与国内流行株的亲缘关系最近,而分离自另一鸡场的7株MS与中东地区分离的3株MS亲缘关系较近,表明MS在川西地区肉鸡场呈高感染率,MS VlhA基因变异较大。本研究为川西地区MS的进一步研究提供了基础材料和科学依据。 相似文献
18.
E J Bacon S J Richmond D J Wood P Stirling B J Bevan W S Chalmers 《The Veterinary record》1986,119(25-26):618-620
Antiserum prepared against a phage which infects a Chlamydia psittaci isolate recovered from domestic ducks was used to screen other recent avian C psittaci isolates by indirect immunofluorescence. Two more phage infected strains from ducks were discovered. However, phage was not detected in every isolate examined from common source ducks, although such birds are likely to be infected with the same C psittaci strain. Moreover, phage could not always be demonstrated by indirect immunofluorescence in McCoy cell monolayers infected with the phage-containing strain. The results suggest that phage infection is probably an integral part of duck chlamydiosis in the United Kingdom at present, but that the infection is often cryptic. 相似文献
19.
新疆部分地区牛羊源产志贺毒素大肠埃希菌菌株检测与分析 总被引：3，自引：2，他引：1
产志贺毒素大肠埃希菌（Shiga toxin-producing Escherichia coli，STEC）是一类携带了前噬菌体编码的一种或两种志贺毒素基因的新发高致病性食源性病原菌，已成为威胁人类健康的重要公共卫生问题。为了解新疆部分地区牛、羊源各个环节产志贺毒素大肠埃希菌的感染情况及其遗传多样性，以及分离株对17种常见抗生素的敏感性，笔者采用PCR方法对STEC分离株进行了4种毒力基因（stx1、stx2、eae、hlyA）的检测和ERIC-PCR基因分型研究。结果表明：从屠宰场、养殖场和市场共431份样品中分离出产志贺毒素的大肠埃希菌64株，其中，编码stx1+stx2的STEC有31株（48.4%），只编码stx1的STEC有29株（45.3%），只编码stx2的STEC有4株（6.3%），4种毒力基因同时存在的有1株。药物敏感性检测发现STEC菌株对麦迪霉素（61%）、头孢噻吩（4.7%）、头孢西丁（4.7%）、氨苄西林（3.1%）、哌拉西林（1.6%）、妥布霉素（1.6%）、头孢唑啉（1.6%）等7种抗生素存在耐药。ERIC-PCR检测结果呈多态性分布，分为A（36株）和B（28株）两个簇。STEC菌株在新疆部分地区牛、羊源各个环节被检出，其中一些菌株可能会增加对食物的污染，从而引起人发病。 相似文献
20.
ZHENG Xiaofeng ZHANG Yan LIU Yingyu ZHU Mingyue ZHENG Xiaoqin LU Wei JIANG Jindou ZHU Menghan 《畜牧兽医学报》1956,51(10):2518-2527
Shiga toxin-producing Escherichia coli(STEC) is a new class of highly pathogenic food-borne pathogens carrying a prephage encoding one or two Shiga toxin genes. It has become an important public health issue that threatens human health. The present work aimed to characterize STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, in terms of the presence of prevalence, genetic diversity, and antimicrobial susceptibility to 17 common antibiotics. Through amplification of four virulence genes (stx1, stx2, eae, hlyA)by PCR and ERIC-PCR genotyping to detection STEC isolates. In the present study, a total of 64 STEC strains were isolated from 431 samples from slaughterhouses, farms and markets. Of these, 31 (48.4%) of the isolates harbored stx1 + stx2, and only 29 (45.3%) of the isolates possessed stx1, only 4 (6.3%) of the isolates harbored stx2, and 1 isolates harbored all the 4 virulence genes. Drug sensitivity tests found that STEC strains displayed 7 antimicrobial resistance to midecamycin(61%), cephalothin(4.7%), cefoxitin(4.7%), ampicillin(3.1%), piperacillin(1.6%), tobramycin(1.6%), cefazolin(1.6%). The ERIC-PCR results showed a polymorphic distribution, which was divided into two clusters of A (36 strains) and B (28 strains). STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, some of which might have the potential to cause food contamination and human diseases. 相似文献