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1.
Stigma exsertion is an important trait for outcrossing ability in rice. Stigma exsertion rate (SER) of male sterile lines (MSLs) is a key factor affecting F1-seed production in hybrid rice. In this study, seven QTLs for SER were detected on five chromosomes using a set of single-segment substitution lines (SSSLs) derived from O. glaberrima. Three of the QTLs were mapped in the estimated intervals of 92.5–333.0 kb. qSER-5 was located in a substitution segment of 92.5 kb. qSER-1b and qSER-8b were respectively limited to 333.0 kb and 107.5 kb by secondary substitution mapping. qSER-1b and qSER-3 had bigger additive effects of 11.5% and 11.9%, respectively, while the other five QTLs had smaller additive effects from 5.7% to 8.6%. Open reading frames were identified in the regions of qSER-5 and qSER-8b in O. sativa and O. glaberrima genomes. Fine mapping of the QTLs laid a foundation for the cloning of genes, and QTLs for SER will be used to develop MSLs with strong ability of outcrossing.  相似文献   

2.
Rice (Oryza sativa) plants acquired excess photosynthates in the form of nonstructural carbohydrates (NSCs) in their stems and grain. Despite keen interest in rice NSC, the dynamics of NSC accumulation, translocation and re-accumulation have not yet been well investigated. AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds. Here we conducted three experiments, greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice (DSR) system, using elite lines incorporating AG1, AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On (AG1) and Ma-Zhan Red (AG2). In germinating seedlings, soluble sugars increased, while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil. Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage. But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017. Plant survival rate was positively correlated with the stem NSC at the early vegetative stage (21 days after sowing), and stem NSC was positively associated with plant height at different growth stages. Among the tested seeding rate, the most suitable seeding rate, 4 g/m2 with shallow burial depth (0.5 cm), resulted in better seedling establishment, relatively higher seedling vigor index and higher leaf area index under flooding in DSR system. Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate, germination rate, and growth and biomass production.  相似文献   

3.
《Field Crops Research》2006,96(1):106-112
Improvement of rice (Oryza sativa L.) plant type is a major breeding objective. This study aimed to precisely localize and characterize key genomic regions for plant type using near-isogenic individuals. Selfing of partially heterozygous F5 recombinant inbred (RI) individuals [parental varieties Milyang 23 (M23) and Akihikari (AK)] developed heterogeneous inbred families (HIFs) composed of 108 and 93 F7 progenies, which segregated at molecular marker loci on the long arms of chromosomes 4 and 6, respectively. The progeny lines (F8) were evaluated for traits composing plant type in Los Baños, Philippines, to locate quantitative trait loci (QTLs) using interval mapping and to evaluate the effects of the QTL region by phenotypic comparison between the genotypes. QTLs for the traits were detected in 17 cM across XNpb12 on chromosome 6. The M23 homozygote for the QTL region resulted in a >7% increase and decrease in plant length and tiller number at heading, respectively, relative to the AK homozygote. Consequently, culm length (CL) and traits determining flag-leaf and panicle sizes increased 5–56% by the M23 homozygote, together with a 15% decrease in panicle number. For a QTL region detected in 6 cM across XNpb235 on chromosome 4, the AK homozygote had similar effects on these traits, except CL. The directions and magnitudes of their effects agreed with those previously estimated in the RI line population, thus increasing confidence in primary QTL analyses for plant type. Analyzing the HIFs validated and characterized the two QTL regions greatly involved in determining varietal plant type from an early growth stage to maturity, providing information useful for empirical and marker-assisted breeding towards rice improvement.  相似文献   

4.
《Field Crops Research》2006,95(2-3):355-366
Nitrogen (N) demand of soybean [Glycine max (L.) Merrill] can be supplied via biological nitrogen fixation (BNF), however, higher yielding cultivars increase plant demand for N. Phenotypes differing for traits associated with biological nitrogen fixation result from the expression of the multiple genes of both the host plant and the microsymbiont, but limited studies have been done on the genetics of the soybean BNF. Integrated maps of soybean with simple sequence repeat (SSR) markers [Cregan, P.B., Jarvik, T., Bush, A.L., Shoemaker, R.C., Lark, K.G., Kahler, A.L., Kaya, N., Van Toai, T.T., Lohnes, D.G., Chung, J., Specht, J.E., 1999. An integrated genetic linkage map of the soybean genome. Crop Sci. 39, 1464–1491.] offer an excellent opportunity for the identification of traits related to BNF. This study aimed at the identification of quantitative trait loci (QTLs) controlling BNF and nodulation in an F2 population of 160 plants derived from an intraspecific cross between two Brazilian cultivars, Embrapa 20 × BRS 133, previously identified as having good potential for mapping of QTLs [Nicolás, M.F., Arias, C.A.A., Hungria, M., 2002. Genetics of nodulation and nitrogen fixation in Brazilian soybean cultivars. Biol. Fertil. Soils 36, 109–117.]. From 252 SSR markers tested in the parental genotypes 45 were polymorphic with high heterozygotes resolution. Mapping was performed with those 45 SSR markers for nodulation [nodule number (NN) and nodule dry weight (NDW)] and plant growth [shoot dry weight (SDW)] phenotypes in F2:3 lines. A total of 21 SSR loci were mapped with a likehood of odds (LOD) value of 3.0 and a maximum Haldane distance of 50 cM, and were distributed in nine linkage groups with coverage of 251.2 cM. The interval mapping analysis with Mapmaker/QTL revealed two genomic regions associated with NN and NDW, with a contribution of putative QTLs of 7.1 and 10%, respectively. The regression analysis identified 13 significant associations between the marker loci and QTLs due to additive effects, with some of them being significantly associated with more than one phenotypic trait. Effects were observed in all variables studied, ranging from 2 to 9%. A one-way analysis of variance (ANOVA) also detected 13 significant associations, related to dominance effects. A two-way-ANOVA showed six epistatic interactions among non-linked QTLs for SDW, NN and NDW, explaining up to 15% of the trait variation and increasing the phenotypic expression from 8 to 28%. The data obtained in this work establish the initial stage for additional studies of the QTLs controlling BNF and indicate that effective marker-assisted selection using SSR markers may be feasible for enhancing BFN traits in soybean breeding programs.  相似文献   

5.
《Field Crops Research》2006,95(2-3):367-382
For maximizing water retention and attaining high yields, transplanting into puddled soil (TPR) is often considered the optimal method of rice (Orzya sativa L.) establishment. Alternative management techniques like direct seeding (DSR) and deep tillage have been proposed as mechanisms to improve soil physical properties for subsequent dry-season crops, but the risks to rice are uncertain. In this full factorial study on a valley terrace in Nepal, the influence of tillage (shallow—T1, deep chisel—T2, deep chisel + moldboard plough—T3) and establishment practice (TPR, DSR) on the field water balance and rice performance were evaluated in two adjacent landscape settings (terrace edge “upland”, central terrace “lowland”). Although deep tillage had only modest influences on seepage and percolation (SP) rates in both years (Y1, Y2), landscape placement and establishment practice had significant implications for the water balance (e.g. Y2 SP cm day−1: TPR-lowland = 1.6, DSR-lowland = 2.3, TPR-upland = 4.1, DSR-upland = 6.1). During low rainfall periods, however, soil water potential and drought vulnerability were governed solely by landscape placement. Despite water balance differences, there was little evidence that rice rooting behavior was substantially modified by landscape or establishment method. Weed biomass was higher in DSR, but was uncorrelated with water balance and productivity trends. In Y1, lower SP rates and more days with continuous flooding were positively associated with rice productivity. DSR yields were significantly lower than TPR in both landscape positions, with the lowland outperforming the upland (Y1 mt ha−1: TPR-lowland = 6.4, DSR-lowland = 5.2, TPR-upland = 5.7, DSR-upland = 4.7). To determine if N dynamics were contributing to productivity differences, fertilizer nitrogen was increased from 120 to 150 kg N ha−1 in Y2. Results suggest that DSR performance is comparable – and landscape less important – if nitrogen is non-limiting (Y2 mt ha−1: TPR-lowland = 6.9, DSR-lowland = 6.5, TPR-upland = 7.0, DSR-upland = 6.5); no aspect of the field water balance was associated with yield variability in Y2. For direct seeding in N-deficient farming systems, landscape criteria may prove useful for minimizing production risks by identifying field areas with lower SP rates.  相似文献   

6.
《Field Crops Research》2004,86(1):53-65
Deceleration in rice (Oryza sativa L.) yield over time under fixed management conditions is a concern for countries like Bangladesh, where rice is the primary source of calories for the human population. Field experiments were conducted from 1990 to 1999 on a Chhiata clay loam soil (Hyperthermic Vertic Endoaquept) in Bangladesh, to determine the effect of different doses of chemical fertilizers alone or in combination with cow dung (CD) and rice husk ash (ash) on yield of lowland rice. Two rice crops—dry season rice (December–May) and wet season rice (July–November) were grown in each year. Six treatments—absolute control (T1), one-third of recommended fertilizer doses (T2), two-thirds of recommended fertilizer doses (T3), full doses of recommended fertilizers (T4), T2+5 t CD and 2.5 t ash ha−1 (T5) and T3+5 t CD and 2.5 t ash ha−1 (T6) were compared. The CD and ash were applied on dry season rice only. The 10-year mean grain yield of rice with T1 was 5.33 t ha−1 per year, while the yield with T2 was 6.86 t ha−1 per year. Increased fertilizer doses with T3 increased the grain yield to 8.07 t ha−1 per year, while the application of recommended chemical fertilizer doses (T4) gave 8.87 t ha−1 per year. The application of CD and ash (T5 and T6) increased rice yield by about 1 t ha−1 per year over that obtained with chemical fertilizer alone (T2 and T3, respectively). Over 10 years, the grain yield trend with the control plots was negative, but not significantly, both in the dry and wet seasons. Under T3 through T6, the yield trend was significantly positive in the dry season, but no significant trend was observed in the wet season. The treatments, which showed positive yield trend, also showed positive total P uptake trend. Positive yield trends were attributed to the increasing P supplying power of the soil.  相似文献   

7.
Combining ability and heterosis were studied in a 6 × 6 diallel cross to see the nature of gene action in Upland cotton (Gossypium hirsutum L.) during 2002 to 2004. Analysis of variance revealed highly significant differences among all the F1 and F2 hybrid means and their respective six parental values for all the traits examined. In both generations, the mean squares due to general combining ability (GCA) and specific combining ability (SCA) were also highly significant. SCA genetic variances were greater than GCA and more important for the traits, i.e. boll weight, boll number and seed cotton yield per plant, showing the predominance of non-additive gene action. Lint % in both generations and boll weight in F2s only were controlled by additive type of gene action due to maximum GCA variances. Cultivar CIM-1100 was found to be the best general combiner and its utilization produced valuable hybrids with desirable SCA in both generations. F1 and F2 hybrids, viz., CIM-1100 × CRIS-9, CIM-1100 × FH-682, CIM-1100 × BH-36 and CIM-109 × CIM-1100 as high × low and low × high parents performed well in SCA determination, outstanding mean performance and heterosis. Better SCA effects associated with useful heterosis were more pronounced for yield traits. In F1 hybrids, maximum heterosis was observed for seed cotton yield followed by boll number, boll weight and lint %. The heterosis over better parent was +3.13 to +65.63% for bolls per plant, +0.75 to +24.40% for boll weight, +0.82 to +115.22% for seed cotton yield and +0.27 to +3.88% for lint %. Involvement of CIM-1100 in most of the F1 and F2 hybrids resulted in the synthesis of superior genotypes for most of the traits studied. Inbreeding depression was elevated in good performing hybrids and was the highest for seed cotton yield. Highest yielding F1 hybrids yielded lesser in the subsequent generation due to over-dominance and inbreeding depression, whereas moderate yielding F1 hybrids were found more stable even passing through process of segregation due to additive gene action. The combined performance of F1 and F2 hybrids could be a good indicator to identify the most promising populations to be utilized either as F2 hybrids or as a resource population for further selection.  相似文献   

8.
《Field Crops Research》2005,91(1):71-81
Wheat (Triticum aestivum L.) cultivation in no-till soil of a postrice harvest field utilizes residual soil moisture and reduces the time period from rice harvest to wheat seeding in intensive rice-wheat cropping systems. Some of the major constraints in no-till wheat production are high weed infestation, poor stand establishment due to rapid drying of topsoil and low nitrogen use efficiency (NUE). A field experiment was conducted at the research farm of the Wheat Research Centre, Dinajpur, Bangladesh, for two consecutive years to overcome those constraints, to evaluate rice straw as mulch, and to determine the optimum application rate of nitrogen (N) for no-till wheat. The treatments included 12 factorial combinations of three levels of mulching: no mulch (M0), surface application of rice straw mulch at 4.0 Mg ha−1 that was withdrawn at 20 days after sowing (M1), the same level of mulch as M1 but allowed to be retained on the soil surface (M2), and four nitrogen levels (control 80, 120 and 160 kg ha−1). Rice straw mulching had a significant effect on conserving initial soil moisture and reducing weed growth. Root length density and root weight density of wheat were positively influenced both by straw mulching and N levels. N uptake and apparent nitrogen recovery of applied N fertilizer were higher in mulch treatments M1 and M2 as compared to M0. Also mulch treatment of M1 and M2 were equally effective at conserving soil moisture, suppressing growth of weed flora, promoting root development and thereby improved grain yield of no-till wheat. N application of 120 kg ha−1 with straw mulch was found to be suitable for no-till wheat in experimental field condition.  相似文献   

9.
Breeding program strategies to develop novel short grain white rice varieties such as japonica (short grain) that introgress biotic stress resistance and high grain quality have been developed using indica rice (Pin Kaset + 4 and Riceberry) for applications in japonica rice (Koshihikari) improvement. Four breeding lines showing promising agronomic performance with short grain and low amylose content (< 20%) were obtained. In addition, sensory testing of these breeding lines showed high scores that similar to Koshihikari. Two promising lines, KP48-1-5 and KP48-1-9, which possessed a combination of four genes resistance to different biotic stresses (Bph3 + TPS + Xa21 + Pi-ta) and four genes for grain quality (GS3 + SSIIa + wxb + badh2), were developed using marker-assisted selection (MAS) with the pedigree method. The current study clearly illustrated the successful use of MAS in combining resistance to multiple biotic stresses while maintaining a high yield potential and preferred grain quality. Moreover, the results indicated that this breeding program, which includes crossing temperate japonica with indica, can create novel short grain rice varieties adapted to a tropical environment, like the japonica type.  相似文献   

10.
Integrated use of organic and inorganic fertilizers can improve crop productivity and sustain soil health and fertility. The present research was conducted to study the effects of application of green manures [sesbania (Sesbania aculeate Poiret) and crotalaria (Crotalaria juncea L.)] and farmyard manure on productivity of rice (Oryza sativa L.) and its residual effects on subsequent groundnut (Arachis hypogaea L.) crop. Rice and groundnut crops were grown in sequence during rainy and post-rainy seasons with and without green manure in combination with different fertilizer and spacing treatments under irrigated conditions. The results showed that application of green manures sesbania and crotalaria at 10 t ha−1 to rice compared to no green manure application significantly increased grain yield of rice by 1.6 and 1.1 t ha−1, and pod yields of groundnut crop succeeding rice by 0.25 and 0.16 t ha−1, respectively. There was no significant difference between the application of crotalaria or farmyard manure at 10 t ha−1 on grain yields of rice, but pod yields of subsequent groundnut crop were greater with application of green manure. There was no significant effect of different spacing 20×15,15×15,15×10 cm2 (333 000; 444 000; 666 000 plant ha−1, respectively) on grain yield of rice. Pod yields of groundnut were significantly greater with closer spacing 15×15 cm2 (444 000 plants ha−1) as compared to spacing of 30×10 cm2 (333 000 plants ha−1). Maximum grain of rice was obtained by application of 120:26:37 kg NPK ha−1 in combination with green manures, whereas maximum pod yield of groundnut was obtained by residual effect of green manure applied to rice and application of 30:26:33 kg NPK ha−1 in combination with gypsum applied to groundnut crop.  相似文献   

11.
In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions at different growth stages were analyzed.The main results were as follows:Allelopathic responses to temperature and light varied with different allelopathic potential rice accessions at different growth stages.With the rise of temperature and the extension of photoperiod,allelopathic effect increased firstly and then decreased at 2–3 leaf stage,but increased constantly at the 4–5 and 7–8 leaf stages in strong allelopathic rice accessions[O.longistaminata,F1(O.longistaminata×RD23),F2(RL159 and RL169)].Temperature had significant impact on allelopathic effect without considering light factors,but light showed little effect on rice allelopathy at the same temperature conditions.The greatest allelopathic effect was attained with moderate temperature and long photoperiod at 2–3 leaf stage in strong allelopathic rice accessions,but all the rice accessions showed weak allelopathic effects at the low temperature condition(15oC/10oC),and the influence of different factors on allelopathy followed a general trend as temperatureleaf stagelight,indicating that among the multiple factors impacting rice allelopathy,temperature was the main factor.Allelopathic characteristics of F1 and F2 to various temperature and light were similar to O.longistaminata,showing that allelopathic genes from wild rice can be expressed in its descendants.Temperature and light also had significant effects on SLA and SMF,and rice allelopathy was closely correlative to SLA in strong allelopathic rice accessions at the 4–5 and 7–8 leaf stages,but there was no correlation between rice allelopathy and SMF at different growth stages.These results suggested that rice adjust the relationship between allelopathy and SLA and adapt to the varied environments,and that high temperature and long photoperiod can enhance rice allelopathic activity.  相似文献   

12.
《Field Crops Research》1999,63(3):187-198
Rice is subjected to excessive waterlogging and flash-flooding on large areas in south and south-east Asia. Besides cultivars, submergence tolerance of plants is influenced by various agronomic practices. A field experiment was conducted at Cuttack, India during 1994–1995 to study the effect of method of stand establishment (direct seeding and transplanting), vigour of seed (low and high-density) or seedlings (N-fertilized and unfertilized), plant population (normal and 50% more) and N fertilizer (single basal and split application) on yield performance of lowland rice under conditions of natural submergence and simulated flash-flooding (impounding up to 90 ± 3 cm depth for 10 days at vegetative stage). Flooding reached a maximum depth of 80 cm in 1994 and 52 cm in 1995 under natural submergence. The crop performance was better in 1994 due to timely sowing in dry soil and delayed accumulation of water (43 days after sowing) than in 1995 when sowing was done late in saturated soil followed by early water accumulation (28 days after sowing). Grain yield of rice decreased by 30.0–33.6% due to simulated flash-flooding compared with natural submergence, and by 21.4–33.1% due to transplanting in July compared with direct seeding in May-end/early June. The yield of direct-sown crop increased by using high-density seed of 22.9–23.0 mg weight (5.2–9.0%), higher seed rate of 600 m−2 (2.2–2.3%) and basal fertilization at 40 kg N ha−1 (19.4–25.7%) compared with low-density seed (19.4–20.1 mg), 400 seed m−2 and no N, respectively. The yield of transplanted crop increased by using N-fertilized seedlings of 0.49–1.65 g weight (29.5–38.5%), higher number of seedlings at 155 m−2 (3.5–16.7%) and basal fertilization at 40 kg N ha−1 (31.9–32.5%) compared with unfertilized seedlings (0.19–0.79 g), 115 seedlings m−2 and no N. Split application of 40 kg N ha−1 — 50% each at basal and top dressing (105–115 days of growth after flash-flooding) — improved yield significantly (10.1–13.1%) over single basal application under simulated flash-flooding, but not under natural submergence conditions. Regression analysis indicated that relative contribution of various factors in increasing grain yield was in order: N fertilizer > seed density > seed m−2 in direct-sown rice, and N fertilizer > seedlings m−2 > seedling dry weight in transplanted rice. It was concluded that grain yield of flood-prone lowland rice can be increased by establishing the crop early through direct seeding using high-density seed and basal N fertilization.  相似文献   

13.
《Field Crops Research》1999,61(3):193-199
The prominent effects of a soil surface crust on crop production, impedance to seedling emergence and reduced infiltration rate, were examined using a quantitative land evaluation model under the Sahelian environmental and soil conditions of north-central Burkina Faso. The model integrated data from climate, soil and crop for quantifying potential grain yield of sorghum (Sorghum bicolor), grown on a sandy loam soil for 14 production years (1977–1990). Crust development was induced using `simulated rainfall' with an intensity of 75 mm h−1 from a 2 m height. Results revealed that seeding sorghum in small holes without sufficiently breaking the surface crust depressed grain yield. Observed and potential yield correlated closely over a 7-year period (r = 0.79, p < = 0.05). Substantial yield gap was found between estimated potential yield (crust broken scenario set to 75% of the predicted yield) and observed, indicating however, the possibility of significantly improving yield by using appropriate tillage to break the crust before seeding.  相似文献   

14.
《Field Crops Research》1999,64(3):273-286
On-farm research to evaluate the productivity and nitrogen (N) nutrition of a rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping system was conducted with 21 farmers in the piedmont of Nepal and with 21 farmers in northwest Bangladesh. In Nepal, two levels of N-fertilizer (0–22–42 and 100–22–42 kg N–P–K ha−1) and farmers’ nutrient management practices were tested in the rice season, and three levels of N (0–22–42, 70–22–42, and 100–22–42) and farmers’ practices were evaluated in the wheat season. The treatments in Bangladesh included a researchers managed minus-N plot (0–22–42) and the farmers’ practices. Rice and wheat yields were higher in all treatments than the 0–22–42 control plots, with the exception of rice with the farmers’ practices at one location in Bangladesh. The researchers’ treatment of 100–22–42 in Nepal resulted in larger yields of both rice and wheat than the farmers’ practices, indicating that farmers’ rates of N-fertilizer (mean 49 kg N ha−1) were too low. Delaying wheat seeding reduced yields in the fertilized plots in both countries, especially as N-fertilizer dose increased. Soil N-supplying capacities (SNSC), measured as total N accumulation from the zero-N plots (0–22–42), and grain yields without N additions were greater for rice than for wheat in both Nepal and Bangladesh. Higher SNSC in rice was probably due to greater mineralization of soil organic N in the warm, moist conditions of the monsoon season than in the cooler, drier wheat season. However, SNSC was not correlated with total soil N, two soil N availability tests (hot KCl-extractable NH4+ or 7-day anaerobic incubation), exchangeable NH4+ or NO3−. Wheat in Nepal had greater N-recovery efficiency, agronomic efficiency of N, and physiological efficiency of N than rice. Nitrogen internal-use efficiency of rice for all treatments in both countries was within published ranges of maximum sufficiency and maximum dilution. In wheat, the relationship between grain yield and N accumulation was linear indicating that mobilization of plant N to the grain was less affected by biotic and abiotic stresses than in rice.  相似文献   

15.
The genetic control determining the days to flowering, defined as the number of days from emergence to the beginning of flowering is considered an important characteristic for breeding purpose. We investigated this factor in kenaf (Hibiscus cannabinus L.), as part of an agroindustrial project in northwest Argentina. A diallelic cross approach was considered in this study. Six highly inbred photosensitive cultivars were used in the cross, namely, Endora, Pandora, Tainung 1, Line 42, Line 21, and Line 29. Significant differences among F1 family means as well as among general combining ability (GCA) and specific combining ability (SCA) components were found based on the Griffing genetic-statistical method IV, Model 1. A predominant additive effect was detected for the days to flowering, giving high heritability estimates (H = DGD = 0.96; h2 = 0.69), and suggests the possibility of effective selection for earliness in these cultivars. Early flowering in Line 29 was highly heritable, and therefore, is important for breeding purposes. Line 42, despite being the earliest, did not transmit this characteristic to its progenies, possibly because of epistatic genetic effects. The regression of the covariances of F1 families on the non-recurrent parent (Wr) and the variance of the “n” families (Vr) revealed that some dominance effects also occurred in the form of a partial dominance for early flowering. These results support the evidences revealed by the analysis of means of combinations between early and late flowering lines.  相似文献   

16.
The objectives of this study were to investigate the mechanisms of action and to evaluate the potential application of Desmos chinensis extracts for controlling rice sheath blight. The dichloromethane extract from D. chinensis demonstrated high antifungal activity against Rhizoctonia solani. The extract was shown to have anti-R. solani activity with minimum inhibitory concentration (MIC) and minimum fungicidal concentration values ranging from 31.2 to 62.5 μg mL−1 and from 62.5 to 500 μg mL−1, respectively. Bioautography on thin-layer chromatography plates demonstrated antifungal activity of the extract with an Rf value of 0.33. A total of 7 compounds, 2 benzoate esters (benzyl benzoate and benzyl 2-hydroxybenzoate), 2 sesquiterpenoids (α-eudesmol and β-eudesmol), 1 aromatic alcohol (benzyl alcohol), 1 aromatic ketone (acetophenone) and 1 diterpenoid (phytol), were detected and identified using gas chromatography–mass spectrometry analysis. Electron micrographs confirmed the effects of the extract on morphological and ultrastructural alterations in the treated fungal cells. The micrographs of the mycelia treated with the extract at 4MIC illustrated aberrant morphology such as shrinkage, partial distortion and globular structures of different sizes along the surface of the mycelia. Damaging membranous structures including disruption of the cell membrane, partial loss of nuclear membranes, and depletion of hyphal cytoplasm and membranous organelles were observed. Foliar application of D. chinensis extract at a concentration of 2 mg mL−1 on rice markedly decreased sheath blight severity. It was concluded that the application of D. chinensis extract can be used as a botanical fungicide to control rice sheath blight.  相似文献   

17.
《Field Crops Research》2005,93(1):94-107
Bangladesh is currently self sufficient in rice (Oryza sativa L.), which accounts for approximately 80% of the total cropped area, and 70% of the cost of crop production. However, farmers are increasingly concerned about the perceived decline in productivity, expressed as the return on fertiliser inputs. Agronomic efficiency is a measure of the increase in grain yield achieved per unit of fertiliser input that can provide a way to quantify the observation of farmers. This study indicates that the yields achieved where only P and K fertiliser were applied ranged from 3–5 t ha−1, indicating good soil fertility, particular in terms of soil N supply (37–112 kg N ha−1). However, at recommended rates and at rates used by farmers, the yield response to application of fertiliser N was low. Data shows that grain yields were significantly correlated in both years (R2 = 0.77 and R2 = 0.67) with plant uptake in nitrogen. The internal nitrogen use efficiency seems to confirm that sink formation was limited by factors other than nitrogen. Low agronomic efficiency (5–19 kg grain kg−1 N) was caused by poor internal efficiency (45–73 kg grain kg−1 N), rather than low supply of soil N or loss of fertiliser N. Thus, often the applications of large amounts of N fertiliser (39–175 kg N ha−1) by farmers to increase yields of high yielding variety Boro rice were not justified agronomically and ecologically. A rate of 39 kg N ha−1 is very low, hardly an environmental threat. No one single factor could be identified to explain the low internal efficiency. Therefore, it is concluded that the data presented tend to confirm the indication that yields are limited by a factor other than nitrogen, which could be crop establishment, plant density, water or pest management, micro-nutrients deficiency, poor seed and transplanted seedling quality, varieties and low radiation.  相似文献   

18.
《Field Crops Research》2006,95(1):75-88
Long-term trends of crop yields have been used as a means to evaluate the sustainability of intensive agriculture. Previous studies have measured yield trends from long-term rice–rice and rice–wheat experiments in different sites from the slopes of individual site regressions of yield over time. The statistical significance of each site regression was determined but not that of the aggregate trend, which could give an indication of the magnitude and significance of global yield change.The random regression coefficient analysis (RRCA) and meta-analysis were used in this study to analyze the aggregate yield trend from several long-term experiments (LTE) across the Indo-Gangetic Plains (IGP) and outside the IGP. Both methods show that there has been a significant (p < 0.05) declining trend in rice yield in rice–wheat LTEs in South Asia including China with the recommended rates of nutrients, but that there has been no significant change in wheat and system (rice + wheat) yields. There was no significant year × region (IGP versus non-IGP) interaction in rice and wheat yields. However, RRCA showed that the average yield trend was significantly negative (−41.0 kg ha−1 yr−1) only in the IGP. In the rice–rice LTEs, there was a significant year × site (IRRI versus non-IRRI sites) interaction during the dry season but not the wet season. Rice yields declined throughout Asia in the wet season. The average system (dry + wet season rice) yield trends were significantly negative in both IRRI and non-IRRI sites (−170.1 and −52.8 kg ha−1 yr−1, respectively) but the magnitude of yield decline was significantly greater in the IRRI sites than in the non-IRRI sites.Rice in the rice–wheat LTEs showed a significantly positive yield trend with the addition of farmyard manure (FYM) but the initial yield was generally lower with FYM than without FYM. After 15 years, yield increase due to FYM was not evident in most of the LTE.  相似文献   

19.
Steam explosion of corn stalk in the presence of 3% sulphuric acid at 200 °C for 5 min gave the highest recovery of lignin. Lignin has Mw = 2640 and Mz = 93,994. In the UV spectrum absorptions at λ = 231 and 280 nm were recorded. 1H NMR spectrum of lignin showed signals attributable to cinnamaldehyde units, guaiacyl units, and syringyl units. Syringyl and guaiacyl units are in 1:1 ratio. 13C NMR spectrum showed signals for guaiacyl, syringyl, and p-hydroxyphenyl units. The spectrum showed a prevalence of guaiacyl units. The 13C NMR spectrum is in agreement with the presence of cinnamic units. The same characterization was performed on lignin from pine. The irradiation of lignin from pine from steam explosion process in the presence of oxygen, in conditions described for the formation of superoxide ion, for different irradiation time was followed isolating the lignin and determining the average molecular weight. The experiments showed that, until 8 h irradiation, Mn decreases, while Mw and Mz increases. After 8 h irradiation an inverse behaviour was observed, with an increase of Mn and a decrease of Mw and Mz. These results are in agreement with an initial polymerization process followed by a photoinduced degradation. Ozonization was carried out in acetonitrile–methanol solution. The reaction showed a zero-order kinetics. After 50 min the average molecular weight of lignin is the half. The reaction mixture was analyzed by using GC–MS. Oxalic acid was determined.  相似文献   

20.
《Field Crops Research》2005,91(2-3):307-318
A 3-year field experiment examined the effects of non-flooded mulching cultivation and traditional flooding and four fertilizer N application rates (0, 75, 150 and 225 kg ha−1 for rice and 0, 60,120, and 180 kg N ha−1 for wheat) on grain yield, N uptake, residual soil Nmin and the net N balance in a rice–wheat rotation on Chengdu flood plain, southwest China. There were significant grain yield responses to N fertilizer. Nitrogen applications of >150 kg ha−1 for rice and >120 kg ha−1 for wheat gave no increase in crop yield but increased crop N uptake and N balance surplus in both water regimes. Average rice grain yield increased by 14% with plastic film mulching and decreased by 16% with wheat straw mulching at lower N inputs compared with traditional flooding. Rice grain yields under SM were comparable to those under PM and TF at higher N inputs. Plastic film mulching of preceding rice did not affect the yield of succeeding wheat but straw mulching had a residual effect on succeeding wheat. As a result, there was 17–18% higher wheat yield under N0 in SM than those in PM and TF. Combined rice and wheat grain yields under plastic mulching was similar to that of flooding and higher than that of straw mulching across N treatments. Soil mineral N (top 60 cm) after the rice harvest ranged from 50 to 65 kg ha−1 and was unaffected by non-flooded mulching cultivation and N rate. After the wheat harvest, soil Nmin ranged from 66 to 88 kg N ha−1 and increased with increasing fertilizer N rate. High N inputs led to a positive N balance (160–621 kg ha−1), but low N inputs resulted in a negative balance (−85 to −360 kg ha−1). Across N treatments, the net N balances of SM were highest among the three cultivations systems, resulting from additional applied wheat straw (79 kg ha−1) as mulching materials. There was not clear trend found in net N balance between PM and TF. Results from this study indicate non-flooded mulching cultivation may be utilized as an alternative option for saving water, using efficiently straw and maintaining or improving crop yield in rice–wheat rotation systems. There is the need to evaluate the long-term environmental risks of non-flooded mulching cultivation and improve system productivity (especially with straw mulching) by integrated resource management.  相似文献   

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