共查询到20条相似文献，搜索用时 171 毫秒
1.
咪噻宁的化学译名为金属巯基组氨酸三甲基内盐(Metallothionein或简写为MT)本文根据其读音,简译为咪噻宁把经过超速离心后的肝细胞浆,通过葡聚糖Sephadex G 75或G 100的凝胶层析柱层析,对所接收的凝洗物用原子吸收光谱测定其中的铜和锌的含量,就可发现有三个峰值金属浓度,其分子量由大渐小,第一峰的分子量大于75,000,第二峰分子量约为 相似文献
2.
《中国奶牛》2015,(5)
为了解锌、铁、钙、镁四种矿物元素与奶牛不孕症之间的关系,对23头供试奶牛分别采集其乳汁10m L、全血8m L(两份),其中一份用于制备血清。乳汁、全血、血清经微波消解处理后,利用日立Z-2000原子吸收分光光度计,通过火焰原子吸收法检测样品中锌、铁、钙、镁四种矿物元素的含量。结果显示,患有不孕症的奶牛钙的含量偏高,与正常值差异显著;患有发情不规律的奶牛钙、铁的含量偏高,与正常值差异显著;患有酮病的奶牛钙、铁、锌的含量偏高,与正常值差异显著;镁的含量与正常值接近,无显著性差异。可以得出,钙与不孕症的发生有很大关系,其含量偏高会增加奶牛不孕症发生的几率,锌、铁与奶牛酮病的发生有关系,而镁对奶牛不孕症的影响很小。 相似文献
3.
镉可通过肠道内二价金属离子转运蛋白1(DMT1)的介导进入动物机体,并与机体内其他二价金属元素(如铁、锌、铜等)的吸收转运存在相互作用。本试验旨在探究稻米来源镉对生长育肥猪组织重金属沉积规律的影响,为寻求降低镉在机体内残留的措施提供参考。选用初始体重为(31.57±2.82)kg的健康生长猪28头,随机分为体重没有明显差异的两组,即对照组(Cd,0.15 mg/kg)与试验组(Cd,0.55 mg/kg),每组14头;饲喂期结束时,生长阶段取6头,生长肥育阶段取8头,分别屠宰;现场测定屠宰性能、器官指数,取各组织样品测定二价重金属的沉积量。结果显示,试验组与对照组相比,无论生长和生长育肥阶段,屠宰性能和器官指数均无显著差异。重金属沉积方面:生长阶段,肝脏中锌含量显著降低,铁含量显著增高,铜含量显著降低;肾脏中锌含量显著降低,铁含量无显著差异,铜含量显著降低。生长育肥阶段,肝脏中锌含量显著增高,铁含量显著降低,铜含量无显著差异;肾脏中锌含量显著增高,铁含量显著降低,铜含量显著增高。其余组织中其他二价金属元素的沉积量并无显著变化。由此表明,较低剂量的稻米来源镉主要影响铁、锌、铜在肝脏及肾脏的分布规律。 相似文献
4.
5.
饲粮锌和钙水平对蛋鸡组织中矿物元素含量的影响 总被引：1，自引：0，他引：1
在玉米—豆饼型基础饲粮中添加不同水平锌和钙,研究了饲粮锌和钙水平对蛋鸡不同组织中九种矿物元素含量的影响。结果表明,肝脏、胰脏和胫骨中锌含量对饲粮锌水平的反应比其它组织敏感。高锌饲粮使血液钙、镁、锰、铜和磷含量,输卵管磷和钾含量显著降低。高钙饲粮使血液铜,肝脏锌、铜、锰、镁、磷、钾和钠含量,胰脏铜含量,肌肉锌含量,胫骨镁、锰、铁、铜和钾含量显著降低,但使血液和肺脏磷含量显著提高。 相似文献
6.
为阐明铜缺乏奶牛甲状腺激素的变化特征及补铜对奶牛甲状腺功能的影响，本实验选择缺铜地区全血铜低于NRC下限（0.8mg/kg)但无明显摇摆症状的犊牛25头作为亚临床铜缺乏组；并对其中15头进行补铜试验（饲料中添加硫酸酮100mg/kg干重），以15头临床健康奶牛作为对照组，分别于40天及80天采集血样并检测T3、T4及TSH；同时测试了有明显摇摆症状的6头发病奶牛及健康奶牛的肝脏中T4－5’脱碘酶的活性。结果表明：铜缺乏奶牛血清T3显著低于亚临床及健康对照组；发病组及亚临床组奶牛血清T4含量显著高于对照组；对照组奶牛血清TSH含量显著高于发病组及亚临床组；发病奶牛肝脏中的T4－5’脱碘酶活性也显著低于健康奶牛；试验组奶牛在补铜后40天血清T3含量显著上升；在补铜后80天，亚临床组奶牛血清T4含量显著下降，TSH含量显著高于补铜前。结论：铜缺乏能够影响奶牛甲状腺激素的调节及代谢过程。 相似文献
7.
8.
拉萨河谷冬春季放牧与舍饲彭波半细毛羊牧草、血液及被毛矿物质含量 总被引：1，自引：0，他引：1
为分析拉萨河谷冬春季放牧与舍饲彭波半细毛羊采食牧草、血液及被毛中主要矿物质元素的含量,试验选用1.5岁左右彭波半细毛羊母羊60只,随机分为放牧组(GZ)和舍饲组(IF)进行试验。结果表明:试验所用牧草矿物质元素,除钼外其他元素含量均为绿麦草>冬季天然牧草。冬季天然牧草磷、镁、硫、铜、锰、锌和硒及绿麦草镁含量低于正常范围。(2)舍饲组血清钙(P=0.026)、硫(P=0.030)、铜(P=0.011)和钼(P=0.014)含量高于放牧组,镁(P=0.090)、铁(P=0.071)、锌(P=0.083)和碘(P=0.066)含量有增高的趋势;其被毛钙(P=0.003)、磷(P=0.006)、镁(P<0.001)、铁(P=0.022)、锰(P<0.001)和碘(P=0.012)含量均高于后者,铜含量有增高的趋势(P=0.068)。在血液和被毛中,放牧组血清铜、锰及被毛铜、锰和硒含量及舍饲组血锰及被毛锰、硒含量均低于推荐值。以上结果表明,拉萨河谷冬春季放牧彭波半细毛羊钙、铁、钼和碘充足,但磷、镁、硫、铜、锰、锌和硒缺乏或不足;舍饲(以谷物为主的精补料+绿麦草干草)能有效解决磷、镁、硫、铜、锌和硒的缺乏问题,但锰仍需补充。 相似文献
9.
为研究奶牛养殖场(户)所用浓缩饲料或精料补充料中微量元素铁、铜、锰和锌的营养状况,利用原子吸收光谱法对从唐山市奶牛养殖场和市场上采集到的浓缩饲料和精料补充料样品中铁、铜、锰和锌含量进行测定,并根据奶牛典型饲料配方进行盈缺分析.结果显示:对于产奶牛用浓缩饲料或精料补充料,锰和锌营养能满足产奶牛营养需要,而铁和铜营养偏高,在配制时应适当降低铁和铜的添加量;对于育成牛用浓缩饲料或精料补充料,铁、铜、锰和锌含量均较高,已超出育成牛的营养需要量范围,在配制时应适当降低添加量;对于干奶牛用浓缩饲料或精料补充料,锰和锌含量能够满足干奶牛营养需要,铁和铜含量高于干奶牛营养需要量,在配制时应适当降低添加量. 相似文献
10.
《动物营养学报》2020,(8)
本试验旨在研究饲粮添加蛋氨酸锌或氧化锌对新生犊牛锌代谢的影响。试验选取36头体重相近的新生荷斯坦母犊牛,随机分成3组,每组12头。对照组无添加,蛋氨酸锌组每日每头牛添加457 mg蛋氨酸锌(相当于80 mg锌),氧化锌组每日每头牛添加104 mg氧化锌(相当于80 mg锌)。试验期14 d。结果表明:1)饲粮添加氧化锌或蛋氨酸锌均可显著提高犊牛血清中锌含量(P0.05)。饲粮添加氧化锌或蛋氨酸锌对犊牛血清中钙、铜、铁、镁和磷含量无显著影响(P0.05)。2)饲粮添加氧化锌或蛋氨酸锌对犊牛血清中锌代谢相关指标均无显著影响(P0.05)。3)饲粮添加氧化锌有提高犊牛粪便中锌含量的趋势(P=0.079)。综上所述,本试验条件下,饲粮中添加蛋氨酸锌或氧化锌形式的锌对犊牛铜、铁、钙、镁、磷等微量元素代谢无负面影响,对血清中锌代谢相关指标无显著影响。蛋氨酸锌组犊牛血清中锌含量高于氧化锌组,而粪便中锌含量低于氧化锌组,说明有机锌的生物利用率高于无机锌。 相似文献
11.
铜缺乏对乳牛自由基代谢的影响 总被引：1，自引：0，他引：1
为阐明铜缺乏对乳牛体内自由基代谢的影响,本实验选择缺铜地区全血铜低于NRC下限(0.8mg/kg)但无明显摇摆症状的犊牛25头作为亚临床铜缺乏组;并对其中15头进行补铜试验(饲料中添加硫酸铜100mg/kg干重),以15头临床健康乳牛作为对照组,分别于40天及80天采集样并检测全血中自由基的含量及血清中NO(一氧化氮)的含量;同时检测了发病乳牛与对照乳牛(各9头)组织细胞浆中NO的含量。结果表明:发病组及亚临床组乳牛全血自由基含量均显著高于对照组(P〈0.05),各组乳牛血清中NO含量差异均显著(P〈0.05),且发病乳牛大脑、小脑及脊髓NO的含量显著或极显著高于对照组,补铜能够显著降低亚临床缺乏组乳牛全血中的自由基或血清中NO含量。结论:铜缺乏能够影响响乳牛体内的自由基代谢过程。 相似文献
12.
13.
14.
K Mamba K Taniguchi S Kagabu T Makita 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(3):566-573
The effect of zinc deficiency on trace metals in the liver, spleen, kidney, pancreas and duodenum was investigated in the control and zinc-deficient rats at 17 days and 20 days of pregnancy. Zinc-deficient rats fell into limosis after 5 days of pregnancy. The contents of zinc, iron, copper and manganese in the maternal tissues were measured by colorimetry with 5NPPF. The morphological changes of the liver were observed by light and electron microscopy. The contents of zinc in the pancreas and duodenum were less in the zinc-deficient group than in the control at both 17 days and 20 days of pregnancy. The contents of the copper and manganese in the liver, kidney, pancreas, duodenum and spleen, however, were not significantly different from the control. The contents of iron in the liver, spleen and kidney in the deficient group increased greatly at 17 days and 20 days of pregnancy compared with the control group. The combination rate of transferrin with iron also increased significantly in the deficient group. Staining with Berlin blue or Turnbull's blue showed intense reaction to iron around the interlobular connective tissue of the liver in the zinc deficient group at 20 days of pregnancy. Ultrastructurally, the liver of the zinc-deficient rats showed the decrease of glycogen granules and increase of lipid-like granules and lysosomes with various sizes and electron densities. These findings suggest that zinc deficiency causes the increase of iron contents in the various organs during pregnancy, and that there is an intimate interrelationship between zinc and iron in the metabolism of iron during pregnancy. 相似文献
15.
戈壁草原蒙古羊春季羔羊铜缺乏症的研究 《畜牧与饲料科学》2022,43(2):46-50
[目的] 确定内蒙古苏尼特左旗北部地区春季发生的戈壁草原蒙古羊羔羊发病、死亡现象的主要原因。[方法] 选取3户羔羊发病情况最为严重的牧户采样,每户各采集5只发病羔羊的血液样本、放牧草场的饮用水样本,以及羔羊所处环境中3种主要牧草（红砂、珍珠猪毛菜和盐爪爪）样本和土壤样本,同时采集当地种羊场15只相同品种健康羔羊的样本作为对照,测定上述样本中的10种矿物质元素（钙、磷、铁、锰、铜、锌、镁、钼、硒、钴）的含量。[结果] 发病羔羊血液中铜和镁的含量显著（P<0.05）低于同品种健康羔羊,钼的含量显著（P<0.05）高于同品种健康羔羊,其他矿物质元素的含量差异不显著（P>0.05）。发病羔羊所处环境中饮用水的镁和钙含量较高。发病羔羊所处环境中红砂的钼、铁、锰和硒含量均高于文献报道值,其他矿物质元素含量均处于正常范围;盐爪爪的钼、铁、锰、硒、镁和钴的含量高于文献报道值,其他矿物质元素含量均处于正常范围;珍珠猪毛菜、红砂和盐爪爪的铜钼比分别为15.96∶1、0.23∶1和2.34∶1。发病羔羊所处环境中土壤样本的铜、铁、锰、硒、钴含量均低于文献报道值,镁、钙和磷含量均高于文献报道值,钼和锌含量在正常范围内。[结论] 该地区戈壁草原蒙古羊羔羊发病、死亡的原因主要是放牧羊摄入铜不足、钼超标、铁和锰等矿物质元素摄入量过多,导致羔羊对铜的吸收发生障碍,最终引起继发性铜缺乏症。 相似文献
16.
日粮不同锌水平对法国鹌鹑生长发育及组织中微量元素含量的影响 总被引：6，自引：0，他引：6
用2周龄健康法国鹌鹑324只,按体重随机分为9组,每组设3个重复,试验采用玉米-豆饼型基础日粮(含锌26.7ppm),在此基础上,添加9个梯度水平(ppm)的锌(0,25,50,75,100,500,1000,1500,2000),进行为期3周的饲养试验.试验期末,进行血液生化指标测定和组织器官微量元素含量分析.试验结果表明,法国鹌鹑日粮中以添加50ppm和75ppm锌为宜,对增重、采食量和饲料效率均有一定作用.1000ppm锌为最大耐受量,1500ppm锌为其中毒量;在一定锌水平范围内,血清总蛋白和球蛋白含量、碱性磷酸酶(SAP)活性均随日粮锌水平的提高而显著提高(P<0.05),当锌添加量为500ppm时,其达最大值,随后呈降低趋势.血清白蛋白含量不受日粮锌水平的影响.锌与其它微量元素之间的关系依不同组织器官而异,缺乏规律性.日粮补饲锌能显著降低肝脏中铁、铜、锰和肾脏中铁的含量,提高骨骼.血清中锰及肾脏中铜的含量. 相似文献
17.
Patricia C Schultheiss Cathy L Bedwell Dwayne W Hamar Martin J Fettman 《Journal of veterinary diagnostic investigation》2002,14(5):396-402
Concentrations of iron, copper, and zinc were measured in livers of 95 dogs that were suspected of having liver disease. Iron concentrations ranged from 177 to 7,680 ppm (dry weight basis); 54 dogs had iron concentrations greater than the normal concentration of 1,200 ppm. Iron stores were present in Kupffer cells and macrophages but not hepatocytes. The dogs did not have lesions of hemochromatosis. Dogs with high liver iron tended to have high liver copper and inflammatory lesions. High liver copper concentrations usually were associated with hepatocellular necrosis and fibrosis. High liver zinc was found in only 5 animals and was accompanied by histologic inflammatory lesions in one. In humans, increased iron concentration in the liver exacerbates liver damage caused by a variety of insults, and the same may be true for dogs. 相似文献
18.
The serum levels of calcium, phosphorus, magnesium, copper, zinc and iron and of nitric oxide, retinol, and β-carotene were
determined in Sakiz ewes that had experienced an abortion and in healthy controls. Ten healthy and 25 aborted Sakiz sheep
were selected from Afyon zone in western Turkey. Their ages ranged between 2 and 4 years weighing between 40 and 60 kg at
the time of experiment. All of the abortions occurred in October. The concentrations of retinol, β-carotene, phosphorus, and
zinc were significantly lower and those of calcium and nitric oxide were increased in aborted ewes relative to healthy controls.
The serum levels of iron, copper, and magnesium were not significantly different among the two groups. In conclusion, abortion
is an important problem in commercially important species of ruminants in many regions in the tropics including of western
Turkey. Deficiencies of retinol, β-carotene, phosphorus and zinc, and the increase of calcium and nitric oxide concentration
may play an important role in the etiology of abortion in ewes. Prophylactic measures such as vitamin and mineral supplementation
may be of help to prevent or reduce the incidence of abortion in sheep. 相似文献
19.
Peripheral nerves, spinal cord, and brain of healthy adult dogs were analysed biochemically for sodium, potassium, calcium, magnesium, copper, zinc, iron, aluminium, silicon, total chloride, total tissue water and total lipid content. Flame atomic absorption spectrophotometry was used for elemental quantitation of the hydrochloric acid tissue extracts. Cerebrum and spinal cord had similar values for all parameters measured. Total sodium values were higher in nerves compared to central nervous system (CNS) (brain and spinal cord), while respective values for potassium were lower. Tissue levels of calcium, iron and silicon were comparable in brain, spinal cord and nerves. However, magnesium concentrations were two to three times higher in CNS than in nerves; and the reverse was true for aluminium levels. Tissue concentrations of zinc and copper were marginally higher in CNS than in nerves. 相似文献
20.
López-Alonso M Prieto F Miranda M Castillo C Hernández JR Benedito JL 《Veterinary journal (London, England : 1997)》2005,170(3):198-338
The intracellular distribution of copper (Cu) and zinc (Zn) in the livers of normal and moderately Cu-exposed cattle was investigated with the aim of improving understanding of the pathophysiology of Cu accumulation in cattle. At total liver Cu concentrations within the generally accepted normal range (25-100 mg/kg fresh weight) the large-granule fraction was the main subcellular compartment for Cu accumulation, followed by the cytosol and the nucleus, whereas in the microsomal fraction Cu accumulation was very low. With increasing Cu exposure, the capacity of the large-granule fraction to accumulate Cu decreased, proceeding towards a plateau (estimated at about 80 mg/kg at a projected total liver Cu concentrations of 450 mg/kg), accompanied by progressively greater Cu accumulation in the nucleus and cytosol. Total liver Cu concentration had little influence on subcellular Zn distribution, with hepatic Cu concentrations being only moderately correlated with Zn concentration in the large-granule fraction. There was a strong association between the proportion of total Cu bound to metallothionein (MT) and the proportion of total Zn in the large-granule fraction, suggesting that Zn displaced from MT is taken up by the lysosomes for excretion. This pattern of Cu accumulation, as in sheep, may be due to the limited capacity for metallothionein binding of Cu and excretion in bile. 相似文献