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1.
半滑舌鳎(Cynoglossus semiliaevis)遗传性别快速准确鉴定的方法是性别控制与高雌苗种培育的基础,其性别决定机制为ZW型,遗传雌鱼为异型染色体ZW,遗传雄鱼为同型染色体ZZ.本研究在半滑舌鳎全基因组测序结果的基础上,通过分析Z染色体和W染色体差异序列,设计一对跨Z/W染色体同源差异DNA片段的引物CS-SEX-F和CS-SEX-R,取半滑舌鳎部分鳍条通过碱煮沸方法获得的DNA为模板,采用PCR扩增,琼脂糖凝胶电泳方法鉴定半滑舌鳎遗传性别.通过该方法在遗传雄鱼(ZZ)个体中可以扩增出366 bp的DNA条带,遗传雌鱼(ZW)个体中可以扩增出366和253 bp两种DNA条带.本研究建立了一种新的成本低廉、操作快速、结果准确可靠的鉴定半滑舌鳎遗传性别的方法,该方法与已有方法相比,操作简便省时,为半滑舌鳎遗传学研究与性别控制育种相关研究提供了可靠分子标记,在半滑舌鳎遗传性别鉴定和养殖场所内伪雄鱼快速检测方面具有广阔的应用前景. 相似文献
2.
半滑舌鳎(Cynoglossus semilaevis)属于雌配子异形染色体性别决定机制(ZW/ZZ),其W染色体包含的大量重复序列阻碍了W染色体序列的精确组装.目前只有物理图谱可以有效地解决由重复序列所造成的组装难题.本研究在已有物理图谱和遗传图谱的基础上,利用3D(three-dimensional)-BAC(bacterial artificial chromosome)池构建和PCR筛选策略进行了性别连锁标记的物理定位.首先将所选44个384孔板的BAC克隆进行接种和预培养,再接种到4个96孔深孔板中.将每两个384孔微孔板的BAC克隆分别汇集获得相应的板池、行池和列池,在提取DNA后,再将相应板池的部分DNA混合得到超级池.最终构建的3D-BAC池,共包括22个超级池和440个基池,覆盖半滑舌鳎基因组4.2倍,完成一个阳性超级池的基池筛选只需24个反应(20个BAC基池和4个对照).然后选用159个半滑舌鳎性别连锁标记对这些克隆池进行两步PCR筛选.在对超级池的筛选中,有142个标记获得阳性扩增.对这些阳性超级池进行基池筛选后,最终有100个标记得到准确定位.定位到半滑舌鳎物理图谱的84个重叠群(contigs)和20个单个克隆(singletons)上,共计1 760个克隆,包含有21 927个共享条带,物理长度为42.4Mb,覆盖半滑舌鳎基因组约5.3％;确定一个定位关系(contig与标记之间的)平均使用了2.2个克隆.在这些标记中,有17个同时定位到了两个或两个以上的contigs上,有11个标记同时定位到contigs和singletons上.另外有19个contigs定位了2个或2个以上标记.这些性别连锁标记的物理定位将有助于半滑舌鳎性染色体基因组序列的精确组装. 相似文献
3.
半滑舌鳎性别特异微卫星标记的SCAR转化及其应用 总被引：3，自引：0，他引：3
半滑舌鳎(Cynoglossus semiliaevis)性别控制与全雌育种相关研究需要一种快速、准确的性别特异分子标记。本研究通过对半滑舌鳎共显性性别特异微卫星标记筛选,得到Z、W染色体同源片段相差35bp的位点,对其进行克隆和测序,针对所得测序结果,重新设计引物进行序列特异扩增区间(sequence characterized amplified region,SCAR)转化,增大35 bp差异片段在扩增片段中的所占比例;并摸索得到4%琼脂糖凝胶,150 V,25 min为最适电泳条件,所得SCAR标记可在雌性个体中扩增出169和134 bp两种DNA条带,雄性个体中扩增出169 bp DNA条带,超雌个体中扩增出134 bp DNA条带;将其应用于生产实践中,对半滑舌鳎生理雄性亲鱼576尾进行快速检测,在保证存活的前提下,剔除了亲鱼中的100尾伪雄鱼,有助于提高后代的雌性比例。本研究所得半滑舌鳎性别特异SCAR标记,可用于实验室对半滑舌鳎雌性、雄性和超雌个体遗传性别的准确测定和养殖场简易环境下养殖亲鱼雄鱼中伪雄个体的快速检测。 相似文献
4.
半滑舌鳎(Cynoglossus semilaevis)是我国沿海地区特有的海水经济养殖品种,在高密度工厂化养殖过程中该品种易受哈维氏弧菌(Vibrio harveyi)感染患腹水病并大量死亡,严重影响了该品种养殖产业的健康发展,本研究以本实验室2016年建立的半滑舌鳎家系为基础,从22个家系中分别选取1 999尾和1715尾295日龄左右的幼鱼进行哈维氏弧菌人工感染实验。实验一共进行288 h,于300 h终止,2次实验感染后存活率范围分别为:0~95.45%和0~87.30%,平均存活率分别为31.17%和26.88%。成对t检验结果表明,2次感染实验中各家系感染后存活率无显著性差异(P=0.210.05)。本研究采用0/1(0表示死亡, 1表示存活)和每尾实验鱼在实验中的存活时间作为半滑舌鳎抗哈维氏弧菌的表型,并利用3种遗传评定模型对该性状进行分析。此外,对各家系育种值(家系中所有个体育种值均值)进行相关性分析和成对t检验以比较3种模型对该性状的估算能力。结果表明,半滑舌鳎抗哈维氏弧菌遗传力为0.17±0.05~0.36±0.20,属中低遗传力。相关性分析结果显示,3种模型估算出的各家系育种值之间的Pearson相关系数范围为0.94~0.97,呈高度正相关关系。3组家系育种值有相似的排名,成对t检验结果表明3组排名之间没有显著性差异(P0.05)。上述结果表明,半滑舌鳎抗哈维氏弧菌属于中低遗传力性状,存在较为丰富的遗传变异,适合采用家系选育的方式对该性状进行选育。此外,3种模型对半滑舌鳎抗哈维氏弧菌遗传参数的估算能力相似。本研究在人工感染实验的基础上,利用3种遗传评定模型分析了半滑舌鳎抗哈维氏弧菌遗传参数,得到了该性状的遗传力和各家系育种值,补充了半滑舌鳎抗哈维氏弧菌遗传参数估计相关内容,为选育抗哈维氏弧菌的半滑舌鳎苗种提供了参考。 相似文献
5.
性别决定是一个可塑的生物发育过程,一直以来都是进化生物学和发育生物学的研究热点。在脊椎动物中,性别决定的机制主要包括遗传性别决定(GSD)和环境性别决定(ESD)。GSD一般都是由位于性染色体上的决定基因启动一系列性别相关基因参与的级联信号通路,从而诱导原始生殖性腺发育成精巢(卵巢)的过程。尽管性别调控信号通路下游的基因在脊椎动物中是相对保守的,但是处于级联信号通路最上游的性别决定基因可能是不稳定的。截止目前,在脊椎动物中,已经发现了5个性别决定基因(SRY、DMRT1、DMY、DMW和AMHY)。本文综述了脊椎动物性别决定基因的研究进展,分析了高等脊椎动物和低等脊椎动物性别决定基因保守性的差异,推测这种差异可能是由于性染色体是否分化造成的,进而提出性别决定基因和性染色体演化关系的模型。 相似文献
6.
短季棉育种是缓解我国当前粮棉争地矛盾、实现粮棉双丰收的有效途径。研究短季棉早熟及相关性状的遗传,寻找与其相关基因紧密连锁的分子标记对于短季棉育种具有重要意义。以百棉2号×TM-1组合构建的F2为作图群体,利用SSR标记构建遗传连锁图,采用复合区间作图法对短季棉早熟及相关性状在F2群体中进行QTL定位,构建了一张含152个SSR标记、40个连锁群、总长1010.3cM、覆盖棉花基因组的22.6%、标记间平均距离为5.91cM的短季棉分子标记遗传连锁图谱,得到的40个连锁群已定位到相应的21条染色体上,为短季棉分子遗传图谱进一步饱和奠定了良好的基础;检测出22个控制早熟及相关性状的QTL,其中检测到的主效QTL可用于短季棉标记辅助选择育种。 相似文献
7.
为了探索玉米株高的遗传机制,定位玉米株高的数量性状位点(QTL),本研究以玉米自交系PH4CV为轮回亲本,以郑58为供体亲本,构建BC1F3:4分离群体,在4个环境下对该群体进行玉米株高表型鉴定。表型分析结果表明,基因型之间差异极显著,且不同环境之间的株高相关性极显著,说明不同环境之间的株高变异具有共同的遗传基础。利用包含5.5万个单核苷酸多态性标记(SNPs)的基因芯片进行基因型鉴定,并结合基因型和表型数据进行全基因组关联分析。在错误发现率(FDR)为0.05时,检测到10个显著性SNPs,这些显著性SNPs主要位于第2号染色体上,-log10(P)值最大的标记为Chr2_194690794。利用线性回归模型对显著SNPs进行表型贡献率及效应分析,发现位于第2号染色体的标记Chr2_194690794效应值最大,贡献率最高,来源于PH4CV的基因型的正效应。利用BC1F5:6群体进行基因型和表型鉴定,进一步确认了标记Chr2_194690794与株高QTL的连锁关系,表明在第2号染色体上存在1个控制株高的QTL。本研究为玉米株高QTL的精细定位奠定了基础。 相似文献
8.
【目的】研究三江源区高寒草甸牧草对不同形态氮素的吸收利用和残留氮素在土壤中的去向及分配,以期为制定三江源区高寒草甸草场养分科学添加方案提供理论依据。【方法】于2020年6月至2021年9月,在青海省称多县高寒草甸试验站开展15N田间微区示踪试验,试验设置3个不同氮素形态处理,分别为(15NH4)2SO4、Ca(15NO3)2、CO(15NH2)2,各处理的氮素施用量均为N 300 kg/hm2。分析了施肥当年和次年不同形态氮素在高寒草甸牧草地上部、地下部中的含量,及在0—15、15—30 cm土层土壤中的去向及分配。【结果】1)在施肥当年,与Ca(15NO3)2、(15NH4)2SO4<... 相似文献
9.
鸡(Gallus gallus)是重要的模式生物和农业动物,性别是其主要的生长发育性状和经济性状.目前鸡性别决定和分化的分子机制还不十分清楚.已有研究证明,尽管鸡有明确分化的性染色体(ZZ/ZW),性别决定和分化主要由遗传决定,但其性腺和成体性别形成和分化一定程度受到环境因素,特别是机体性激素的调控和影响.此前对鸡性别决定和分化机制的解释,主要集中在W染色体显性效应与Z染色体剂量效应两个假说,分别有一定实验证据支持但都不能令人完全信服.本研究在阐述已有假说及其相关研究的基础上,重点介绍了表观遗传学在鸡性别决定和分化研究中取得的最新进展,以及新近发现的鸡性别决定的细胞自主性问题. 相似文献
10.
叶片面积影响光合作用效率,是农作物产量的重要构成性状之一。野生黄瓜(Cucumis sativus var.hardwickii)的叶片较小,经过人工驯化后的栽培黄瓜(Cucumis sativus var.sativus)的叶片面积扩大了2~3倍。前人研究已经将控制黄瓜叶面积的主效基因之一ll(little leaf)定位在第6号染色体上。本研究以黄瓜小叶品系XF-24(P1)、大叶品系DF-32(P2)杂交产生的205个单株的F2群体为研究材料,用SAS软件对成熟期调查的各单株相同节位的叶面积进行正态性检验,结果服从正态分布,符合数量性状的遗传特征。为了有效地加快研究进程,在双亲测序情况下,采用插入缺失位点(insertion and deletion,InDel)标记进行基因定位。研究结合双亲全基因组测序数据,生物信息学分析得出双亲序列之间的InDel位点,用Primer Premier 5.0软件在所有染色体上均匀设计88对InDel标记引物,扩增采用分离群体分组混合分析法(bulked segregant analysis,BSA)组建大叶、小叶基因池,池间有多态的引物再进一步扩增F2群体DNA,筛选到7个与黄瓜叶面积基因ll2连锁的分子标记,位于黄瓜第7号染色体上,分别是InDel-1、InDel-2、InDel-3、InDel-4、InDel-5、InDel-6、InDel-7。建立遗传连锁图谱并进行区间作图寻找QTL位点,结果显示,遗传连锁图谱包含以上7个InDel标记,连锁区间为22.1 cM,包括1个控制黄瓜叶片大小的主效QTL位点ll2(little leaf 2),位于标记InDel-2与InDel-4之间,这两个标记之间物理距离为1.24 Mb。与前人的研究结果相比,定位区间更小且是7号染色体上首次发现的黄瓜叶面积QTL位点。截止目前,在黄瓜6号、7号染色体共发现了2个黄瓜叶面积主效QTL位点,分别是ll和ll2,表明黄瓜叶面积遗传机制复杂。叶面积主效QTL ll2的遗传定位,对于控制黄瓜叶片面积的遗传机制和分子机理研究以及分子标记辅助育种具有重要的意义。 相似文献
11.
Yanbing Li Xueming Xiao Yong Chen 《Communications in Soil Science and Plant Analysis》2017,48(21):2526-2533
Aluminum (Al) toxicity represents one of the main yield-limiting factors for crops in acid soils. Silicon (Si) is known to increase tolerance in higher plants. This study was conducted to determine whether treatment with Si could improve nutrient uptake by peanut under Al stress. Peanut (Arachis hypogaea L. cv Zhonghua 4) was raised with or without Si (1.5 mM) in the growth chamber under 0 and toxic Al (0.3 mM) levels. Aluminum stress significantly decreased the root- and total-dry weight by 52.4% and 32.0%, respectively. The content of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) was significantly decreased, but that of Al increased markedly in shoots and roots after Al exposure at seedling, flower-needle, and pod-setting stage. Silicon alleviates Al toxicity in peanut plants in relation to Al distribution and allocation of tissue P, K, Ca, and Mg by favoring the partitioning of dry mass to roots. 相似文献
12.
很少有关于直接测定强度耕作侵蚀引起土壤退化过程的报道。我们的目的是确定应用137Cs和210Pbex直接定量评价耕作侵蚀对土壤有机质(SOM)坡面运移影响的可能性。我们在黄土高原陡坡耕地上进行了50次犁耕活动,并将与其相临的另一块陡坡耕地作为对照。在对照坡地不同坡位,137Cs浓度均匀分布于上部0～30cm土层,而210Pbex浓度在坡上部和坡中部随土层深度增加呈现线性递减,在坡下部呈指数函数递减。0～30cm土层中土壤有机质含量显著大于30cm深度以下土层,并在坡中部和坡下部呈现与210Pbex类似的土壤剖面分布特征。与对照坡地比较,50次犁耕活动导致坡上部、坡中部0～45cm土层的SOM含量分别降低了38%和47%,坡下部0～100cm土层中的SOM含量却提高了18%。坡上部土壤剖面中137Cs浓度的加权平均值从1.48Bq/kg降低到0.29Bq/kg,坡中部从2.53Bq/kg减少到0.33Bq/kg,坡下部从1.48Bq/kg提高到2.81Bq/kg。210Pbex浓度的剖面加权平均值在坡上部从27.71Bq/kg下降到6.15Bq/kg,在坡中部和坡下部分别从35.46Bq/kg和25.53Bq/kg降低到1.57Bq/kg和19.40Bq/kg。137Cs和210Pbex的剖面分布与SOM在p<0.001水平呈显著相关,相关系数R2值在对照坡地为0.81～0.86,在犁耕实验坡地为0.86～0.91。实验结果表明,环境放射性核素137Cs和210Pbex在黄 相似文献
13.
Kulvir Singh Harinder Pal Singh Sudhir Kumar Mishra 《Communications in Soil Science and Plant Analysis》2020,51(7):919-931
ABSTRACTDelayed sowing and imprecise application of irrigation water to cotton has been the major hurdle in sustaining cotton yield in north-western India. Therefore, studies were initiated to ascertain the impact of heavy or normal level of presowing irrigation (PSI), scheduling time of first postsowing irrigation (POSI) under two sowing dates (SDs) on cotton arranged in a split block design replicated thrice. PSIh (100 mm) recorded 23.2% higher seed cotton yield over PSIn (70 mm) owing to better yield attributes and higher stand. First POSI at 4 weeks after sowing (WAS) resulted the highest yield (3072 kg ha?1), while one at 6 WAS (POSI6) recorded least due to poor population and reduced yield attributes. Water productivity under POSI6 was lesser by 29.1%, 25.2%, and 16.2% as compared to POSI3, POSI4, and POSI5, respectively. April sown crop out yielded the May sown cotton crop by 939 kg ha?1 due to better yield attributes. Nitrogen factor productivity (NFP) among SD remained higher by 23.3% for April as compared to May sowing. PSIh exhibited better NFP over PSIn. POSI6 recorded least NFP by 24.4%, 28.5%, and 16.3% as compared to POSI3, POSI4, and POSI5, respectively and was indicative of relatively poor utilization of N under delayed schedules. Therefore, planting in April after heavy PSI and scheduling first POSI at 4 WAS is best strategy for sustaining cotton yield and maximizing farmer profitability. 相似文献
14.
Luong Bui Bich Hanh Phung Thi My Rinh Pham Dinh Trang Pham Minh Thang Do Trong Stephen Harper 《Communications in Soil Science and Plant Analysis》2019,50(1):102-107
Inductively coupled plasma-mass spectroscopic (ICP-MS) analysis of leaves from 22 cabbage crops in the Sa P? and B?c Hà districts of Láo Cai Province, North-Western Vi?t Nam, revealed unexpectedly high concentrations of chromium (Cr) and nickel (Ni). The concentrations were strongly linearly related (r2 = 0.94), indicating sample contamination during grinding through a stainless-steel hammer mill. We tested this hypothesis in two ways. First, brown rice ground through the same mill was contaminated not only by Cr and Ni, but also cobalt (Co), iron (Fe) and molybdenum (Mo). Second, scanning electron microscopy/energy dispersive analysis of x-rays (SEM/EDS) of the ground samples revealed small fragments with co-located Fe, Cr and Ni, consistent with stainless steel wear fragments. Other grinders may perform differently and we suggest that quality assurance protocols for trace metal analysis of plants should include testing for grinder wear metals. Lastly, brown rice appears to be convenient for investigating contamination of plant tissues during grinding. 相似文献
15.
Hamdi Zurqani Christopher Post Mark Schlautman Julia Sharp 《Communications in Soil Science and Plant Analysis》2018,49(6):689-700
Sodicity and salinity can adversely affect soil structure and are common constraints to plant growth in arid regions. Current remote sensing techniques cannot distinguish between the various classes of salt-affected soils. Field and laboratory measurements of salt-affected soils are time-consuming and expensive. Mapping of the salt-affected soils can be used in soil conservation planning to identify regions with different degrees of limitations. There is a need to use existing field and laboratory measurements to create maps of classes of salt-affected soils. The objectives of this study are to classify salt-affected soils, use existing field data to interpolate and validate geospatial predictions of the classes of salt-affected soils using Geographic Information Systems (GIS), and create maps showing the different classes and distribution of salt-affected soils. The classification framework for salt-affected soils is based on electrical conductivity (ECe), soil pH and the sodium adsorption ratio (SAR), and provides four degrees of limitations to salt-affected soils: slight (normal soils), moderate (saline soils), severe (sodic soils), and extreme (saline-sodic soils). Spatial interpolation of the field data from northwestern Libya was verified by cross-validation, and maps of the salt-affected soils in the region were created. The majority of soils in this region of Libya are normal (slight degree of limitation). Twenty percent of the topsoil is saline-sodic (extreme degree of limitation). Land use recommendations and rehabilitation strategies can be developed from such maps of salt-affected soil classes. The methodology followed in this study can be applied to other arid regions around the world, particularly in developing countries where budgetary constraints limit detailed field and laboratory measurements of sodicity and salinity. 相似文献
16.
华北平原灌溉农田的土壤水量平衡和水分利用效率 总被引：16，自引：1，他引：16
华北平原农业面临的主要问题是水资源短缺,地下水位持续下降。通过一维土壤水量平衡模型模拟了华北平原不同灌溉方式下农田耗水量和土壤水分深层渗漏的变化,并分析了作物的产量和水分利用效率。结果显示在正常的灌溉条件下,冬小麦季地下水的采补差额超过了200mm,某生育期一定程度的水分亏缺(返青期、拔节期或灌浆期)能明显减少冬小麦的耗水量,但没有明显减少作物的产量。因此,在一定程度上减少灌溉是可行的,但仍不能达到地下水资源的采补平衡。从长远来看,华北平原维持可持续的地下水灌溉开采,应减少冬小麦的种植面积、增加低耗水经济作物的比例。 相似文献
17.
模拟降雨条件下污染土壤中重金属元素径流迁移特征 总被引：6，自引：0，他引：6
探明污染土壤中重金属元素随地表径流迁移特征,对于防控重金属污染物的迁移扩散具有重要意义。通过室内模拟降雨试验,研究不同降雨强度下(55,100,120mm/h)污染土壤中Cd、Pb和Cr随地表径流迁移的特征。结果表明:径流中3种重金属元素总量在产流的前20min内逐渐降低,随后趋于稳定;产流初期(产流10min内)颗粒态重金属占径流重金属总量的比例均在80%以上;随产流时间延长,颗粒态Cd和Cr对径流重金属的贡献率快速降低,其颗粒态/溶解态的比值范围分别为47.0～0.4和12.9～0.4,而Pb则主要以颗粒态的形式存在,溶解态Pb对其随径流迁移的贡献率可忽略不计;随降雨强度增大,径流中总Cd和Pb含量显著降低,而径流中总Cr含量以及溶解态重金属含量无明显变化规律。 相似文献
18.
森林土壤有机碳库组分定量化研究 总被引：1，自引：0，他引：1
用一级动力学方程研究了贵州省黎平县森林土壤活性、缓效性和惰效性有机碳库的变化及分解速率,模拟结果表明:各土壤剖面的土壤活性碳库一般占总有机碳的0.5%~7.6%,平均驻留时间(M ean Residue Tim e,MRT)为41~64天;缓效性碳库占总有机碳的45%~71%,平均驻留时间为3~30年;采用酸水解法测定惰效性碳库的库容,一般占总有机碳的20%~50%。活性碳库的变化规律为混交林>阔叶林>针叶林,缓效性碳库中混交林最大,其它两种林分规律不明显;不同林龄的杉木(8年,16年,40年),非活性碳库(缓效性碳库和惰效性碳库之和)的含量变化规律为40年>16年>8年,说明40年生的杉木下土壤固碳能力比8年和16年的强;16年的又比8年的强。 相似文献
19.
Information is scant on the effect of humic acid (HA) on physiological, antioxidant and photosynthesis attributes of gerbera plants undergoing nutrient deficiency in culture solution. Gerbera plants cv. Malibu were grown in a factorial experiment based on a completely randomized design with 3 replications, using 3 different nutrient solutions [complete nutrient solution (NSc), 25% NSc (NS1), and 50% NSc (NS2)] treated with 2 levels of humic acid [0 (HA0) and 500 mg/l (HA1)].The interaction effect of HA and NS showed that HA improved the flower number in NSc, the transpiration in NS1+HA1, photosynthesis rate in NSc+HA1, stomatal conductance (gs) in NS2, mesophyll conductance of leaves in all NS levels and photosynthetic water use efficiency in NSc+HA1. The interaction effect of nutrient solution and HA on antioxidant activity was inconclusive, malondialdehyde content was the highest in NS2 and the lowest in NS1+HA1. The peroxidase activity increased in complete nutrient solution with and without HA and there were no differences among other treatments. Superoxide dismutase activity increased in NS1 and complete nutrient solution with HA and reached the highest in NSc. Humic acid was more effective in nutrient uptake, i.e., nitrogen, phosphorus, potassium, calcium, zinc, and iron (N, P, K, Ca, Zn, and Fe) in complete nutrient solution compared to NS1 and NS2. Conclusively, humic acid can compensate the nutrient deficiency stress of the culture solution in regards to protein synthesis, photosynthesis attributes regardless of the nutrient uptake of gerbera. 相似文献
20.
Water eutrophication and poultry manure require an efficacious remediation technology to decrease phosphorous (P) concentration. In this study, the P phytoremediation potential of a mining (ME) and nonmining ecotype (NME) of Pilea sinofasciata were analyzed by pot experiments employing different concentrations of P treatments (CK, P100, P200, P400, P800 and P1600) in tested soil. Growth, P accumulation and P-relevant enzyme activities of P. sinofasciata in ME and NME were assessed after 10 weeks’ plantation. The results showed that there was no significant difference between the shoot biomass of the ME and NME. Higher P concentration and accumulation were observed in the ME than NME for all treatments. Stem P concentration of the ME were 117%, 189%, 152%, 153%, 140% and 139% higher than the NME, and leaf P concentration were 122%, 146%, 138%, 144%, 137% and 113%. Maximum P uptake and phytoextraction was observed at P400 for the ME of P. sinofasciata. Elevated activities of acid phosphatase and phytase in roots were examined in P-enriched soil, and the ME presented higher activities compared with the NME. The results in this paper provide a theoretical basis for the use of ME of P. sinofasciata for phosphorus accumulators and suggest that ME of P. sinofasciata is a promising species for the excess P phytoextraction of P-enriched soils. 相似文献