共查询到20条相似文献，搜索用时 218 毫秒
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Kentaro Higuchi Yutaka Takeuchi Misako Miwa Yoji Yamamoto Kazunobu Tsunemoto Goro Yoshizaki 《Fisheries Science》2011,77(1):69-77
Recently, we developed an intraspecies spermatogonial transplantation technique in a pelagic egg spawning marine teleost,
nibe croaker Nibea mitsukurii. Nibe croaker is an ideal candidate recipient for spermatogonial transplantation since it has a short generation time and
small body size. In the present study, yellowtail Seriola quinqueradiata spermatogonia were transplanted into nibe croaker larvae, and the behavior of transplanted spermatogonia in recipient gonads
was observed. Three weeks post-transplantation, yellowtail spermatogonia were incorporated into the gonads of 72 out of 88
recipients. An antiproliferating cell nuclear antigen was detected in incorporated yellowtail spermatogonia, suggesting that
the xenogenic germ cells were proliferating in recipient gonads. Yellowtail vasa-positive spermatogonia survived for 11 months after transplantation in the gonads of recipient fish. Thus, we showed that
the microenvironment in nibe croaker gonads can support the colonization, proliferation, and survival of germ cells derived
from a different taxonomic family. 相似文献
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Chris M. C. Woods Philip J. James Graeme A. Moss Johnny Wright Sten Siikavuopio 《Aquaculture International》2008,16(1):49-68
We compared the gonad-enhancing effects of two diets (NIWA and NIFA diets) in two size classes of sea urchins (Evechinus chloroticus): small and large urchins with a test diameter of 75.6 ± 1.2 mm and 93.8 ± 0.5 mm, weighing 174.8 ± 7.9 g and 315 ± 5.6 g,
respectively. After eight weeks being fed prepared diets, urchin gonad index (GI) had significantly surpassed that of the
initial and final samples of wild urchins. Urchins in the NIWA/small treatment had a larger GI than the NIFA/small and NIFA/large
treatments. The NIWA/large treatment had a larger GI than the NIFA/large treatment. The overall percentage increase in GI
for the NIWA/small, NIWA/large, NIFA/small and NIFA/large treatments were 183%, 135%, 132% and 85%, respectively. In terms
of gonad colour (CIELAB), there were no significant differences in gonad redness (a*) or yellowness (b*) between experimental
treatments, but there was a significant difference in gonad lightness (L*) with small urchins fed both diets having lighter-coloured
gonads than the large urchins fed both diets. Sensory assessment of gonads revealed that gonads from small urchins fed both
diets were rated as being of better colouration and more uniform in colour than gonads from large urchins. Gonads of urchins
fed the NIFA diet were rated as being less bitter and of better overall taste than gonads from urchins fed the NIWA diet.
This study shows that feeding prepared diets to E. chloroticus can significantly increase gonad yield but that different diets can affect the magnitude of GI increase and the taste of
gonads, and that smaller urchins appear more suitable for gonad enhancement. 相似文献
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Frederick W. Goetz Edward M. Donaldson George A. Hunter Helen M. Dye 《Aquaculture (Amsterdam, Netherlands)》1979,17(4):267-278
Methods are described for controlling sex differentiation in the coho salmon, Oncorhynchus kisutch. Eyed eggs and alevins were immersed in solutions of estradiol-17β and 17α-methyltestosterone and fed these steroids in the diet for a period of 10 weeks postswim-up. In seven out of 10 groups that received estradiol all fish resembled normal females when sampled at 4 months posthatch. In fish treated with methyltestosterone at all except the lowest dose (25 and 50 μg/l) the gonads resembled neither normal males nor normal females; these gonads were composed largely of connective tissue with only occasional germ cells. Various proportions of male, female and intersex gonads were observed in groups which received androgen or estrogen only in the diet. The implications of the work for salmon culture include the production of sterile fish and increasing the proportion of female salmon in hatchery populations. 相似文献
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M.V. MARTÍN C. RODRÍGUEZ J.R. CEJAS Mª J. PÉREZ S. JEREZ & A. LORENZO 《Aquaculture Nutrition》2009,15(1):60-72
Total lipid (TL), lipid classes and fatty acid composition of neutral (NL) and polar (PL) lipids were studied in the gonads, liver and muscle of gilthead seabream males ( Sparus aurata ) fed a control diet (diet C) or an n-3 and n-6 highly unsaturated fatty acids (HUFA)-deficient diet (diet D), at different stages of the reproductive cycle. Between pre-spermatogenesis (November) and spermatogenesis (March), the lipid content was high and particularly rich in cholesterol, phosphatidylcholine and phosphatidylethanolamine in gonads from both dietary groups. At post-spermatogenesis (June), TL and especially PL dramatically decreased in the gonads from both groups. However, at this period diet C fish gonads were richer in triacylglycerides (TAG) than those from diet D fish. The liver lipid contents and particularly TAG were over 200% lower in June than in March for both groups. Nevertheless, the most noteworthy depletion of lipids during this period was achieved by the n-3 HUFA in diet D fish. Conversely, arachidonic acid (20:4n-6) did not decrease in NL or PL from gonads and liver in groups C and D. Muscle lipids from diet C fish were relatively insensitive to seasonal influences. However, in June, the muscle TAG content was significantly reduced in diet D fish. 相似文献
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大西洋鲑性腺分化及热休克的影响 总被引：7，自引：2，他引：7
大西洋鲑性腺分化发生在孵出45－63天，约495－693度日。经热休克处理获得三倍体稚鱼，性腺在分化之前明显大于正常二倍体。雌性三倍体稚鱼性腺在细胞学分化时受抑，初级卵母细胞不能发育；雄性三倍体稚鱼性腺在细胞学分化阶段生长明显减慢，初级精母细胞尚能发育。 相似文献
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Valerio Zupo Francesca Glaviano Marina Paolucci Nadia Ruocco Gianluca Polese Anna Di Cosmo Maria Costantini Mirko Mutalipassi 《Aquaculture Nutrition》2019,25(1):26-38
Sea urchin gonads are exploited both for gastronomic and scientific purposes; thus, the production of large and mature gonads is essential. Natural populations of the Mediterranean Sea urchin Paracentrotus lividus are subject to increasing fishing efforts, due to continuous intensification of consumptions. Aquaculture practices may represent an answer, but the availability of artificial feeds accelerating the production of high‐quality gonads in terms of size, taste, colour, firmness, maturity and viability of gametes is critical to boost the productions. The accessibility of cheap and effective feeds promoting the fattening of gonads and the quality of gametes is still a bottleneck slowing down the expansion of echinoculture practices. This investigation is aimed at enabling the development of this strategic sector, by comparing the dietetic effects of fresh foods and a commercial feed for aquaculture, to four newly formulated feeds. The protein contents of diets were strongly related to the GSIs. The abundance of fatty acids appeared inversely related to the viability of embryos and abnormalities of larvae. The features of an ideal diet for this sea urchin were defined, based on the results of experimental trials, and the need for increasing levels of plant‐derived proteins during the grow‐out period was demonstrated. 相似文献
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生物酶解是提高水产品蛋白质利用率的有效手段，且酶解过程往往伴随肽类、氨基酸、小分子挥发性成分等的生成或反应，进而引起酶解液风味变化。仿刺参(Apostichopus japonicus)性腺富含蛋白质和多种功效成分，具有良好的开发利用前景。为探究仿刺参性腺酶解过程中蛋白质和风味的变化规律，采用中性蛋白酶对其进行酶解，对酶解过程中可溶性蛋白质、氨基酸态氮、游离氨基酸组成、挥发性成分的变化情况进行了检测分析。结果显示，仿刺参性腺匀浆液中可溶性蛋白质和氨基酸态氮的初始含量分别为1.14和0.15 g/100 g，可溶性蛋白质在酶解前30 min内迅速增加，之后基本保持不变，氨基酸态氮含量在前90 min内随时间延长而逐渐增加，90 min后略有下降，90 min时水解度最大，达43.66%。随着酶解时间的延长，酶解液的鲜味有所增强，腥味减弱，甜味和苦味也略有增强。酶解后游离氨基酸含量显著增加(P<0.05)，其中，呈鲜味的谷氨酸含量最高，其次为呈甜味的甘氨酸和丙氨酸。仿刺参性腺酶解过程气味发生明显变化，烃类种类和相对含量均明显增加，二甲基硫醚含量显著降低，这可能是酶解液腥味减弱的主要原因 相似文献
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Melissa Sullivan Alexandria C. Brown Ethan D. Clotfelter 《Fish physiology and biochemistry》2014,40(5):1399-1405
Carotenoids may act as antioxidants under many circumstances. We examined the importance of carotenoids as antioxidants in the gonads of male convict cichlids (Amatitlania nigrofasciata), a species in which males lack the carotenoid-based breeding coloration that characterizes females. Male fish were fed one of four diets that included different combinations of xanthophyll and carotene carotenoids, and then we measured carotenoid concentration of the gonads, gonadosomatic index (GSI), sperm motility, and the antioxidant capacity of the gonads. Significant differences were found in gonadal carotenoid content among treatment groups, suggesting that dietary carotenoids were indeed sequestered in the gonads. There were no differences among diet groups, however, in GSI, sperm motility, or gonadal antioxidant capacity. These findings suggest that carotenoids are required only in small amounts in the testes of male convict cichlids or that they play a limited role in protecting sperm from oxidative damage. 相似文献
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Piotr Hliwa Mamadou Bah Henryk Kuźmiński Stefan Dobosz Andrzej Ciereszko 《Aquaculture International》2014,22(1):89-96
Ultrasound approach has been used to assess morphology of the gonadal structure in the sex-reversed rainbow trout females (neomales) provided in the course of 11β-hydroxyandrostenedione treatment applied within gonadal differentiation period. Eighteen matured individuals (in range 38.0–48.6 cm of body length and 802–1644 g of body weight) were scanned using digital ultrasound apparatus (DP-6900 model) Mindray Ltd., during the spawning season. After screening, fishes were killed to validate the morphological configuration and position of the gonads in the body cavity. The favorable place for cross-sectional imaging of gonadal lobes was half of the distance between the pectoral and pelvic fins below lateral line of neomales. Most of the examined specimens (61 %) had properly shaped, paired testis. Moreover, presence of individuals with asymmetrical gonads (33 %) and one bisexual fish was confirmed. There were no differences between total volume of sampled semen and sperm motility, but statistically significantly different in sperm concentration within selected (with symmetrical and asymmetrical testis) groups of neomales. It was confirmed that ultrasonic imaging is an efficient and accurate method to determine the state of gonads of mature sex-reversed rainbow trout females during spawning season and offers the opportunity for non-invasive detection of any morphological anomalies in their gonads. 相似文献
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Lei Tang Huan Wang Hongling Wei Chunyu Ye Lizhi Chen Hongzhi Yao Ce Shi Changkao Mu Chunlin Wang 《Aquaculture Research》2020,51(1):29-40
In order to reveal the influence of overwintering behaviour on the flavour of Scylla paramamosain, this study selected crabs of before and after overwintering in SM (Sanmen County, Zhejiang Province), HP (Hepu County, Guangxi Province) and WN (Wanning city, Hainan Province). The contents of flavouring‐related substances in hepatopancreas, muscles and gonads were determined by high‐performance liquid chromatography (HPLC); taste activity value (TAV) and equivalent umami concentration (EUC) were calculated. The results showed that sweetish amino acids were mainly accumulated in muscles, umami amino acids were mainly accumulated in hepatopancreas, free nucleotides were mainly accumulated in gonads, and the EUC value in gonads was the highest. After overwintering, the content of sweetish, umami amino acids and free nucleotides in muscle and hepatopancreas decreased significantly, while the content of flavour‐tasting amino acids in gonads did not change significantly, but the content of umami nucleotides increased significantly. Therefore, overwintering behaviour decreased the flavour of muscle and hepatopancreas, slightly enhanced gonad and the overall flavour of S. paramamosain before overwintering was better than that after overwintering. 相似文献
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多棘海盘车用作新型海洋食品原料的可行性研究 总被引：3，自引：0，他引：3
多棘海盘车（ Ａｓｔｅｒｉａｓ ａｍｕｒｅｎｓｉｓ） 在中国沿海居民中有长期食用的历史,其可食部为生殖腺（ 海星黄） ,但有麻辣感。海星黄含蛋白质达１５ ．４６ ％ ,其氨基酸组成齐全,必需氨基酸间比例合理。海星黄还富含微量元素、维生素、脂肪等营养元素,脂质中不饱和脂肪酸含量尤为丰富。海星黄麻辣味可能由硫酸酯甾体皂甙引起,大部分可通过脱麻辣味及脱毒操作除去。急性毒性试验中,小鼠灌胃８ 和１２ ｇ／ｋｇ 脱毒的海星黄,未见毒性反应;短期喂养试验（１２ ｇ／ｋｇ ,３０ ｄ） 也未见毒性反应。两项试验中,小鼠肝、心、肾、肺和脾均未见异常。建议人的最大无毒剂量１ ｇ／ｋｇ ,最大允许摄入剂量０ ．５ ｇ／ｋｇ 。海星黄经脱麻辣味及脱毒、调味等步骤,制得２ 种食品,具有较好的感观且营养丰富。海星壳所含蛋白质主要为胶原蛋白,以胃蛋白酶促溶蛋白为主,具有典型胶原蛋白氨基酸组成特点,可在食品工业用作明胶。初步结论为,多棘海盘车可用作高营养、无毒、加工利用性好的新型海洋食品原料。 相似文献
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采用磷酸苯二钠法在520nm波长测定了未成熟、早熟和正常成熟中华绒螯蟹肝胰腺与性腺中的酸性磷酸酶（ACP）和碱性磷酸酶（ALP）。结果表明：（1）各组蟹肝胰腺ACP和ALP活性都显著高于性腺;（2）早熟蟹肝胰腺中ACP活性都显著高于未成熟蟹而低于正常成熟蟹,在各发育阶段没有性别差异,但是,早熟雌蟹和正常成熟雌蟹卵巢中ACP活性都较早熟雄蟹和正常雄蟹精巢低,早熟与正常成熟个体没有差异;（3）早熟雌蟹肝胰腺ALP活性显著高于未成熟和正常成熟雌蟹,而与三组雄蟹处于同一水平,但是,早熟和正常成熟雌雄蟹的性腺中ALP活性没有显著差异。由此得出结论：（1）性腺发育所需营养物质的动员或合成主要与肝胰腺ACP有关;（2）性早熟的发生与肝胰腺中ACP和ALP活性的显著升高有关。 相似文献
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渔光一体养殖模式(photovoltaic fishery breeding mode, PM)是一种采用水下养殖、水上发电的新兴环保养殖模式。本研究比较了渔光一体养殖模式与普通池塘养殖模式(common pond breeding mode, CM)养殖中华绒螯蟹(Eriocheir sinensis)在基本营养成分、感官评价和气味品质等方面的差异。对雌蟹肝胰腺和性腺进行人工及电子感官评价,测定挥发性物质的含量,并对气味品质进行综合评价。结果表明,渔光一体养殖模式能提高雌蟹的性腺指数、增加粗蛋白和粗脂肪含量;PM组肝胰腺表现青草味,电子鼻的响应值最高,PM组性腺的青草味和油脂味更明显;在肝胰腺中,鉴定出44种(PM组)和33种(CM组)物质,PM组总挥发性物质的含量[(70.53±2.91) ng/g]显著高于CM组[(16.63±0.73) ng/g](P<0.05),是CM组的4.2倍,其中油脂味和青草味的物质含量更高;而在性腺中,鉴定出了31种(PM组)和47种(CM组)物质,PM组和CM组总挥发性物质含量分别为(68.80±1.86) ng/g和(71.50±4.17)... 相似文献
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Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeon Huso huso Linneaus, 1758 during maturity developmental stages 
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下载免费PDF全文 Mahtab Yarmohammadi Mohammad Pourkazemi Rezvanollah Kazemi Mohammad Ali Yazdani Sadati Ali Hallajian Mohammad Hassanzadeh Saber 《Aquaculture Research》2017,48(4):1413-1429
Little is known about molecular mechanisms involved in gonad differentiation in sturgeon species. In non‐mammalian vertebrates, sox9, dmrt1, cyp17a1 and ar are male specific genes in testicular differentiation and are highly conserved. In order to understand the mechanism underlying testicular development in sturgeon, we investigated sex steroids level of 11‐ketotestosterone (KT) and testosterone (T) and relative expression of sox9, dmrt1, cyp17a1 and ar in great sturgeon gonads during different stages of sexual maturity. The results showed all studied genes had dimorphic patterns in males. In immature gonads (stage I), only cyp17a1 expressed in male gonads, while other genes did not express, and KT and T levels were low. Dmrt1 showed dimorphic expression pattern in male gonads at maturity stage II, III and IV. Furthermore, sox9 and ar mRNA presented significant dimorphic expression pattern in male gonads only at maturity stage 4. Plasma androgens levels were significantly higher in males compared to females during maturity stages II, III and IV. The results showed that among these four genes, only cyp17a1 expressed in male gonads at maturity stage I (immature), suggesting that this gene may be applicable as a sex marker in recently differentiated male great sturgeon. Sexually dimorphic patterns in other studied genes (sox9, dmrt1 and ar) suggesting that these genes may be important for testicular development and differentiation in premature great sturgeon. The obtained results provide a foundation for further research on sex differentiation and developing strategies for the sexing of sturgeon for aquaculture. 相似文献
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The effects of water temperature on the development of hermaphroditic gonads in red seabream (Pagrus major) and on mRNA expression of cytochrome P450 aromatase (P450arom) and 11β-hydroxylase were examined. High water temperature suppressed both expression of P450arom and 11β-hydroxylase and the development of oocytes in ovarian portion of hermaphroditic gonads. 相似文献
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本试验对中华绒螯蟹"蟹黄"与"蟹膏"的粗蛋白、氨基酸、粗脂肪、脂肪酸、卵磷脂和维生素等营养成分进行了测定分析。试验结果表明,蟹黄与蟹膏中的粗蛋白质含量为29.79%和9.64%,蟹黄显著高于蟹膏(P0.05);至少共含有17种氨基酸,蟹黄中的必需氨基酸显著高于蟹膏(P0.05),4种致鲜氨基酸比例明显高于蟹膏(P0.05);蟹黄、蟹膏中粗脂肪含量为44.3%和34.9%,差异不显著(P0.05),不饱和脂肪酸含量丰富,蟹黄高于蟹膏,差异不显著(P0.05);蟹黄中卵磷脂、维生素A、D、E的含量均显著高于蟹膏(P0.05)。试验表明,蟹黄比蟹膏具有更高的营养与食用价值。 相似文献
20.
Change in Sox9 protein localization through gonad development in Russian sturgeon (Acipenser gueldenstaedtii) 下载免费PDF全文
下载免费PDF全文 Maciej Kamaszewski Aleksandra Gosk Marek Skrobisz Teresa Ostaszewska 《Aquaculture Research》2017,48(6):3111-3120
The Russian sturgeon is a highly prized species reared in aquaculture. The process of gonad development in this critically endangered species is poorly understood. The aim of this study was to describe the localization of Sox9 protein during gonad development of the Russian sturgeon from the day of hatching to the 1440 day post hatching (dph). The larvae at age 1, 10, 25 dph and prepared gonads of 300, 720, 1440 dph individuals were immunohistochemistry‐stained for Sox9 detection. Sox9‐positive regions were detected in larvae in primordial germ cells cytoplasm. Analysis of 300 dph sturgeon gonads revealed the presence of the Sox9 protein in cytoplasm of some oocytes in the chromatin nucleus stage. In testes at 720 dph, Sox9 was observed in the cytoplasm of type A and early B spermatogonias. In the ovaries, Sox9 was observed in the cytoplasm of diplotene oocytes and prefollicular cells. In testes of 1440 dph sturgeon, Sox9 was present in the nucleus of the spermatocytes and in types A and B spermatogonias cytoplasm. Analysis of ovaries at 1440 dph reveals multiple diplotene oocytes with a Sox9‐positive cytoplasm. Furthermore, in 720 and 1440 dph, sturgeon presence of intersexual gonads was detected. In intersex gonads, Sox9 was observed in the cytoplasm of diplotene oocytes and type A spermatogonias. This study may be the first attempt to determine Sox9 protein localization during ontogenesis of the Russian sturgeon. Localization of Sox9 protein may become a useful marker of the maturation level in testis of the Russian sturgeon. 相似文献