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1.
Jue Lou Liang Chen Gaohong Yue Qiaojun Lou Hanwei Mei Liang Xiong Lijun Luo 《Journal of Cereal Science》2009,50(2):145-151
Grain quality improvement is one of the most important goals in a rice breeding program. An indica variety with small grain size was crossed to a japonica variety with large grain size to construct a set of recombinant inbred lines (RILs) which was used to identify quantitative trait loci (QTLs) controlling eight grain quality traits. Based on a linkage map of 185 SSR markers, a total of 16 QTLs were mapped on six chromosomes. A pleiotropic main effect QTL (M-QTL) flanked by RM3204 and RM16 on chromosome 3 influences the grain length (GL), length width ratio (LWR) and head rice ratio (HRR), explaining the phenotypic variation of 46.0, 36.1 and 29.7%, respectively. A total of 18 epistatic QTLs were identified for all the traits except MRR, distributed on all the chromosomes except chromosome 10. Two M-QTLs for GL and one M-QTL for GW were involved in epistatic QTL. No significant interaction between M-QTL or epistatic QTL and environment was detected except AC having significant M-QTL by environment interaction with minor effect. GL and LWR have a significant negative relation with HRR which might make it difficult to develop long grain with higher HRR in the rice breeding practice. 相似文献
2.
Shaohong Zhang Xiuying He Junliang Zhao Yongsheng Cheng Zhimei Xie Yuehan Chen Tifeng Yang Jingfang Dong Xiaofei Wang Qing Liu Wei Liu Xingxue Mao Hua Fu Zhaoming Chen Yaoping Liao Bin Liu 《Rice》2017,10(1):44
Background
Harvest index (HI) in rice is defined as the ratio of grain yield (GY) to biomass (BM). Although it has been demonstrated that HI is significantly related to yield and is considered as one of the most important traits in high-yielding rice breeding, HI-based high-yielding rice breeding is difficult due to its polygenic nature and insufficient knowledge on the genetic basis of HI. Therefore, searching for rice varieties with high HI and mapping genes associated with high HI can facilitate marker-assisted breeding for high HI in rice.Results
Yuexiangzhan, a popular indica cultivar with good reputation of high HI was crossed with Shengbasimiao, an indica cultivar with lower HI to develop a recombinant inbred line population, and QTL mapping for HI and its component traits was conducted. In total, five QTLs for HI, three QTLs for GY, and six QTLs for BM were detected in two-year experiments. Among the three GY QTLs, one co-located with the HI QTL on chromosome 8, while the other two co-located with the two tightly-linked BM QTLs on chromosome 3. The co-located QTLs in each of the chromosomal regions produced additive effects in the same direction. Particularly, the HI QTL on chromosome 8, qHI-8, could be detected across two years and explained 42.8% and 44.5% of the phenotypic variation, respectively. The existence of qHI-8 was confirmed by the evaluation of the near isogenic lines derived from a residual heterozygous line, and this QTL was delimitated to a 1070 kb interval by substitution mapping.Conclusion
In the present study, the detected GY QTLs overlapped with both HI QTL and BM QTL, suggesting a positive relationship between GY and HI or BM, respectively. With an understanding of the genetic basis for grain yield, harvest index and biomass, it is possible to achieve higher yield through enhancing HI and BM by pyramiding the favorable alleles for the two traits via marker-assisted selection (MAS). As qHI-8 has a large phenotypic effect on HI and expresses stably in different environments, it provides a promising target for further genetic characterization of HI and MAS of high HI in rice breeding.3.
Genetic relationship between grain yield and the contents of protein and fat in a recombinant inbred population of rice 总被引：1，自引：0，他引：1
Yong-Hong Yu Gang Li Ye-Yang Fan Ke-Qin Zhang Jie Min Zhi-Wei Zhu Jie-Yun Zhuang 《Journal of Cereal Science》2009,50(1):121-125
To study the genetic relationship between grain yield and the nutrient contents in rice, 209 recombinant inbred lines derived from a cross between indica rice Xieqingzao B and Milyang 46 were used to determine quantitative trait loci (QTLs) affecting the yields and contents of the two major nutritional components in brown rice. Seven traits were analyzed, including brown rice recovery (BRR), protein content (PC), fat content (FC), grain yield (GYD), brown rice yield (BRYD), protein yield (PYD) and fat yield (FYD). The nutrient contents were significantly negatively correlated with BRR, GYD and BRYD, and the variations on nutrient yield were mainly ascribed to GYD. A total of 22 QTLs distributed on 10 regions of eight chromosomes were detected. Two QTL clusters were found on the short arm of chromosome 6 and the long arm of chromosome 10, respectively. The former was responsible for all the seven traits, and the latter for all the traits except BRR and FC. In both regions, the maternal alleles decreased nutrient contents, but they increased the yields of grain, brown rice, protein and fat. Implication of these results for the breeding of rice varieties with enhancing nutritional capacity is discussed. 相似文献
4.
水稻灌浆期耐热害的数量性状基因位点分析 总被引：14，自引：1，他引：14
利用由98个家系组成的Nipponbare / Kasalath // Nipponbare回交重组自交系群体及其分子连锁图谱，以粒重感热指数\[（适温粒重-高温粒重）/适温粒重×100\]为评价指标，采用混合线性模型的QTL定位方法，对水稻灌浆期耐热性的主效、上位性数量性状基因位点及其与环境的互作进行分析。共检测到3个灌浆期耐热性主效QTL，分别位于第1、4和7染色体上，LOD值为8.16、11.08和12.86，贡献率8.94%、17.25%和13.50%。其中位于第4染色体标记C1100-R1783之间的QTL，没有显著的上位性和环境互作效应，表明在不同环境和遗传背景中的表达较为稳定，在水稻耐热性育种中可能具有较大的利用价值，其耐热性等位基因来自亲本Kasalath，高温热害时可减少粒重损失3.31%。位于第1染色体标记R1613-C970之间的QTL和第7染色体标记C1226-R1440之间的QTL，耐热性等位基因来自亲本Nipponbare，分别可减少粒重损失2.38%和2.92%。这两个QTL均具有与环境的互作效应，其中第7染色体上的QTL还和其他基因位点有互作。检测到8对加性×加性上位性互作QTL，分布于第1、2、3、5、7、8、10和12染色体上。没有检测到上位性QTL与环境的互作效应。 相似文献
5.
不同年份水稻产量性状的QTL分析 总被引：7，自引：1，他引：6
分别于2005年和2006年利用热研2号（粳稻）和密阳23（籼稻）为亲本构建的含111个家系的F6和F7重组自交系群体,对单株穗数、每穗颖花数、每穗实粒数、结实率、千粒重和单株产量6个性状进行了QTL分析。两年共检测到分布于7条染色体上的19个QTL,其中2005年检测到10个,2006年检测到14个,两年相同的QTL 5个。大多数性状之间具有显著的表型相关性,相关性较强的性状之间具有较多共同或紧密连锁的QTL。检测到3个控制产量性状的QTL区域存在一因多效或紧密连锁,其中第8染色体上RM5556-RM331区域两年同时检测到控制每穗颖花数、每穗实粒数以及单株产量的QTL。这些QTL为通过分子标记辅助选择提高水稻产量提供了有用信息。 相似文献
6.
为了解西藏半野生小麦粒型性状的QTL差异,以西藏半野生小麦Q1028和郑麦9023(ZM9023)杂交后获得的重组自交系群体为试验材料,于2012、2013和2015年分别在四川农业大学温江试验田种植,对其粒型性状(粒长、粒宽、粒厚、长宽比、籽粒大小)进行遗传分析。结果表明,重组自交系群体粒型性状均呈正态分布,对籽粒大小的影响依次为粒宽、粒厚、粒长。在三个年度环境中,总共检测到33个控制小麦粒长、粒宽、粒厚、籽粒大小和长宽比的QTL位点。其中,13个控制粒长的QTL分布在1B、2B、2D(3个)、3A、4A、5B、6A、6B、7A(3个)染色体上,每个位点对表型变异的贡献率为5.37%~11.57%。6个控制粒宽的QTL分布在2B、2D、4A、5B、6A、7A染色体上,可以解释表型变异的6.43%~12.69%。3个控制粒厚的QTL位于2B和2D(2个)上,表型贡献率分别为12.75%、10.00%和8.49%。9个控制籽粒大小的QTL分别位于2B、2D(2个)、4A、5B、6A、7A(3个)染色体上,单个QTL可解释6.26%~14.69%的表型变异。另外,本研究还在2B、2D、4A、5B、6A、7A染色体上共发现7个QTL富集区,这些染色体上的QTL和富集区与籽粒性状密切相关,在育种中值得关注。其中,2B染色体上XwPt-3561~XwPt-6932分子标记区间内有控制粒长、粒宽、粒厚、籽粒大小的遗传位点,6A染色体上标记wpt-730109与wpt-7063之间有控制增加籽粒宽度和籽粒大小的位点。 相似文献
7.
ZHANG Qiao-feng ZHANG Ya-dong ZHU Zhen ZHAO Ling ZHAO Qing-yong XU Ling WANG Cai-lin 《水稻科学》2008,15(3):186-194
The rice starch viscosity characteristics, which can be indicated by Rapid Visco Analyzer profile (RVA profile), have been proved useful for the evaluation of cooking and eating quality in rice breeding program. To study the inheritance of the RVA profile, an F2 population of Wuyujing 3/Aichi 106 was used. The results indicated that the peak viscosity (PKV) was a typical quantitative character, and the hot paste viscosity (HPV), cool paste viscosity (CPV), setback viscosity (SBV), breakdown viscosity (BDV), and consistence viscosity (CSV) might be controlled by one major gene and several minor genes.To elucidate the genetic basis of the paste viscosity characteristics, a recombinant inbred line (RIL) population derived from Nikken 2/Milyang 23 and its genetic linkage map were used to map the QTLs controlling RVA profiles in 2005 and 2006. A total of 34 QTLs distributed on chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected, including 19 and 15 QTLs in 2005 and 2006,respectively. Eight QTLs were both detected in the two years, qHPV6, qCPV6, qCSV6, qSBV6, and qBDV6were located on chromosome 6, while qHPV2, qCSV2, and qCPV2 were on chromosome 2. 相似文献
8.
为剖析水稻叶片气孔性状的遗传机理,以典型籼稻七山占和典型粳稻秋光构建的F10重组自交系群体为材料,用该群体包括122个SSR标记的遗传连锁图谱对水稻齐穗期剑叶气孔性状进行了QTL分析。共检测到9个控制气孔相关性状的QTL。包括4个控制气孔密度的QTL(qSD-1、qSD-4、qSD-7和qSD-8),分别位于第1、4、7和8染色体上,贡献率分别为7.2%、8.1%、9.1%和5.9%;2个控制气孔长度的QTL(qSL-4和qSL-6),位于第4和6染色体上,贡献率分别为6.6%和10.5%;3个控制气孔宽度的QTL(qSW-5、qSW-7和qSW-10),位于第5、7和10染色体上,贡献率分别为12.4%、9.7%和5.6%。与其他研究结果相比较发现,除qSD-1外,其余8个QTL过去均未曾报道过,其中位于第4染色体上控制气孔密度的qSD-4与前人检测到的控制光合速率的QTL重叠。 相似文献
9.
Rapid prediction of head rice yield and grain shape for genome-wide association study in indica rice
Rice milling and appearance qualities are the main determinants of market price of the milled rice, so great breeding efforts have been directed to improve the milling and appearance quality. The success of breeding efforts depends on the accurate and rapid phenotyping of the quality traits in early generations. This study was conducted to first validate the effectiveness of the PaddyCheck to measure head rice yield (HRY) and paddy grain length of 40 indica rice cultivars. The results indicated that the PaddyCheck data had positively correlated with the lab methods of HRY (r = 0.81**) and grain length (r = 0.97**). An association panel including 281 indica rice accessions was phenotyped with the PaddyCheck and the data were used for genome-wide association study (GWAS) to identify quantitative trait locus (QTL) for milling and appearance quality. A total of nine QTLs were identified, among which the major genes GS3 and qSW5 were identified for grain length and width, respectively. Furthermore, a novel QTL on short arm of chromosome 6 was identified for HRY. Our study indicated that PaddyCheck measurement was accurate and effective, and could be applied in rice breeding for improvement of grain shape and milling quality. The nine QTLs identified in indica rice could be used in marker assisted selection in rice breeding programs. 相似文献
10.
用培矮64S/日本晴F2群体对水稻6个农艺性状的QTL定位 总被引：1，自引：0，他引：1
用水稻测序品种培矮64S和日本晴配组建立了由180个单株组成的F2群体，构建了含137个SSR标记的连锁遗传图谱，对水稻的分蘖数、有效分蘖数、分蘖率、株高、剑叶长和穗长等6个相关农艺性状进行了QTL定位分析。共检测到14个QTL，分布在第1、2、4、5、6、7染色体的11个区间。检测到1个控制株高的主效QTL(qPH1 2),位于第1染色体，其表型贡献率为24.0%；1个控制剑叶长的主效QTL(qFL4）,位于第4染色体，其表型贡献率为30.5%。对所定位QTL的价值、QTL在染色体上的区域分布等进行了探讨。 相似文献
11.
Inheritance and QTL Mapping of Salt Tolerance in Rice 总被引：6，自引：0，他引：6
An F2 population derived from the cross between Jiucaiqing (japonica) and IR36 (indica) was used to analyze the inheritance of salt tolerance in rice by genetic model of major-genes plus polygenes, and to map the corresponding QTLs by SSR molecular markers. Rice plants of P1, P2, F1 and F2 at 5- to 6- leaf stage were treated under 140 mmol/L NaCI for 10 days. Three indices representing the ability of salt tolerance of rice seedlings were measured, including salt tolerance rating (STR), Na^ /K^ ratio in roots and dry matter weight of shoots (DWS). STR, Na^ /K^ and DWS were all controlled by two major genes with modification by polygenes. Heritability of these traits from major genes was 17.8, 53.3 and 52.3%, respectively. The linkage map constructed by 62 SSR molecular markers covered a total length of about 1 142 cM. There were three QTLs detected for STR located on chromosome 1, 5 and 9, two QTLs for DWS on chromosomes 8 and 9, and two QTLs for Na^ /K^ on chromosomes 2 and 6, one on each chromosome respectively. Single QTL accounted for 6.7 to 19.3% of phenotypic variation. Identification method of salt tolerance in rice and breeding of rice varieties with salt tolerance based on molecular markers assisted selection had been discussed. 相似文献
12.
稻米淀粉黏滞性(RVA谱)特征值的遗传及QTL定位分析 总被引：2，自引：1，他引：1
利用武育粳3号/Aichi 106构建的F2 群体,研究了精米粉RVA谱8个特征值: 最高黏度(peak viscosity,PKV)、热浆黏度(hot paste viscosity,HPV)、冷胶黏度(cool paste viscosity,CPV)、崩解值(breakdown viscosity,BDV)、消减值(setback viscosity,SBV)和回复值(consistence viscosity,CSV)、峰值时间（peak time, PeT）、糊化温度（pasting temperature, PaT）的遗传规律。结果表明, PKV为由微效基因控制的数量性状;HPV、CPV、SBV、BDV、CSV可能是由一对主基因和微效多基因共同控制的数量性状;PeT可能由两对主基因控制,并且还受微效多基因的影响。用热研2号/密阳23构建重组自交系群体,于2005-2006年对稻米淀粉RVA谱8个特征值进行了数量性状基因座QTL定位和分析,两年试验共检测到34个QTL,分布在第1、2、3、4、6、7、8共7条染色体上;两年同时检测到的QTL共8个,其中qHPV6、 qCPV6、 qCSV6、 qSBV6、qBDV6位于第6染色体的Wx基因附近;qHPV2、qCSV2位于第2染色体RM341与RM475标记之间,qCPV2位于RM573与RM250标记之间。第2染色体上与支链淀粉合成有关的两个基因与RVA谱特征值有密切关系。 相似文献
13.
【目的】挖掘新的稻米品质性状QTL并利用分子育种技术改良稻米品质。【方法】利用构建的一套以籼稻香型恢复系昌恢121为背景亲本,以优质粳稻越光为供体亲本的染色体片段代换系（CSSLs）为材料,在4个环境下对稻米品质性状进行QTL检测及稳定性分析。【结果】在4个环境下共检测到44个QTL,其中6个QTL能在多个环境下被检测到;第2、3、5、6和10染色体上存在多效QTL簇,对稻米品质性状具有明显的调控作用;第1、6和12染色体上7个QTL能在不同环境下稳定表达。【结论】第1染色体RM3143-RM1117区间qPGWC1和第12染色体RM3331-RM5479区间qPaT12是两个新的稳定表达QTL。 相似文献
14.
不同环境下稻米品质性状QTL的检测及稳定性分析 总被引：1，自引：0，他引：1
【目的】挖掘新的稻米品质性状QTL并利用分子育种技术改良稻米品质。【方法】利用构建的一套以籼稻香型恢复系昌恢121为背景亲本，以优质粳稻越光为供体亲本的染色体片段代换系（CSSLs）为材料，在4个环境下对稻米品质性状进行QTL检测及稳定性分析。【结果】在4个环境下共检测到44个QTL，其中6个QTL能在多个环境下被检测到；第2、3、5、6和10染色体上存在多效QTL簇，对稻米品质性状具有明显的调控作用；第1、6和12染色体上7个QTL能在不同环境下稳定表达。【结论】第1染色体RM3143−RM1117区间qPGWC1和第12染色体RM3331−RM5479区间qPaT12是两个新的稳定表达QTL。 相似文献
15.
利用近等基因系研究3个抗水稻纹枯病QTL的聚合效应 总被引：2，自引：0，他引：2
利用特青/Lemont组合的回交群体,证实了特青第7染色体上存在抗纹枯病QTL qSB7Tq。以Lemont为轮回亲本,通过分子标记辅助连续回交结合性状鉴定,构建了qSB7Tq与另外两个抗水稻纹枯病QTL qSB9Tq（位于特青第9染色体）和qSB11Le（位于Lemont第11染色体）的一套近等基因系,对各抗性QTL的单个效应及其聚合效应进行了研究。3个抗性QTL单独存在或在聚合状态下均能显著提高水稻品种对纹枯病的抗性水平。但是,不同抗性QTL之间可能普遍存在一定的负向互作关系。讨论了一致的遗传背景对QTL研究的重要性,以及不同QTL的聚合效应及互作关系对育种实践的意义。 相似文献
16.
LV Yang WANG Yueying Noushin JAHAN HU Haitao CHEN Ping SHANG Lianguang LIN Haiyan DONG Guojun HU Jiang GAO Zhenyu QIAN Qian ZHANG Yu GUO Longbiao 《水稻科学》2019,26(6):384-392
Excavating single nucleotide polymorphisms(SNPs) significantly associated with rice grain shape and predicting candidate genes through genome-wide association study(GWAS) can provide a theoretical basis for discovery and utilization of excellent genetic resources in rice. Based on 16 352 SNPs, 161 natural indica rice varieties with various grain sizes in southern China were used for GWAS of grain shape-related traits, referring to grain length(GL), grain width(GW), 1000-grain weight(TGW), and grain length/width(GLW). Phenotypic statistics showed that coefficient of variation values for these four traits GL, GW, TGW and GLW were 9.92%, 9.09%, 20.20% and 16.38%, respectively. Each trait showed a normal distribution, and there was a certain correlation between these traits. Through general linear model correlation analysis, a total of 38 significant loci were identified, and a range of 100 kb upstream and downstream of the significant loci was identified as the candidate interval. On chromosome 3, GS3 and q GL3 were found to regulate GL. On chromosome 6, TGW6 and GW6 a were found to regulate TGW. Also, some QTLs related to grain shape were found on chromosomes 5 and 9. Besides that, using sequenced 3 K-germplasm resources, we found that there are 22 overlapped varieties between these two natural populations. Twenty-six SNPs and fourteen haplotypes were identified in five regions of GS3 genes. The detection of multiple candidate genes/QTLs within the candidate interval is beneficial for further excavation of superior rice genetic resources. 相似文献
17.
18.
《Journal of Cereal Science》2004,40(2):109-114
Identification and mapping of genomic regions controlling quantitative trait loci (QTLs) was undertaken to determine the genomic regions associated with milling traits in rice to facilitate breeding of new rice varieties with high milling quality. The recombinant inbred (RI) population used was derived from cross of a japonica variety, ‘Asominori’, with an indica variety, ‘IR24’ through 289 RFLP markers. Three milling traits, namely, brown rice percentage (BRP), milled rice percentage (MRP), and milled head rice percentage (MHP), which are the main indicators of milling quality in rice, were estimated for each RI line and their parental varieties. Continuous distributions and transgressive segregations of three milling traits were observed in the RI population, showing that the three traits were quantitatively inherited. Two QTLs (qBRP-9 and qBRP-10) for BRP were identified and mapped to chromosomes 9 and 10, and explained 7.2 and 21.3% of the total phenotype variation, respectively. Two QTLs (qMRP-11 and qMRP-12) governing MRP were detected and mapped to chromosomes 11 and 12, accounted for 12.2 and 7.7% of total phenotype variation, respectively. In addition, three QTLs (qMHP-1, qMHP-3 and qMHP-5) controlling MHP were observed and mapped to chromosomes 1, 3 and 5, and explained 16.0, 22.1 and 8.7% of the total phenotype variation, respectively. Among them, five QTLs (qBRP-9, qBRP-10, qMRP-11, qMHP-3 and qMHP-5) from japonica parent, Asominori, and two QTLs (qMRP-12, qMHP-1) from indica IR24 can improve milling quality in rice. The results and the tightly linked molecular markers that flank the QTL will be useful in breeding for improvement of milling quality in rice. 相似文献
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20.
多环境下粳稻产量及其相关性状的条件和非条件QTL定位 总被引：1，自引：0，他引：1
为了剖析粳稻产量及其相关性状的遗传基础,利用粳稻品种秀水79×Ｃ堡衍生的重组自交系群体,在3个环境下对全生育期、株高、单株穗数、每穗粒数、百粒重、籽粒产量和生物产量进行了非条件和条件QTL定位。共检测到43个主效QTL和29对上位性QTL。利用非条件QTL定位方法检测到37个主效QTL和26对上位性QTL。其中,籽粒产量定位到3个主效QTL qGY1.2、qGY7.1和qGY9,未检测到上位性QTL。利用条件QTL方法分别将全生育期、株高、穗数、每穗粒数、百粒重和生物产量各自调整到同一水平后,籽粒产量共检测到9个主效条件QTL和3对上位性QTL,其中3个主效QTL与非条件下定位到的相同。位于第9染色体长臂区间RM6570-RM5652的qGY9在非条件及全生育期、株高、穗数、粒数和百粒重调整到同一水平后均可检测到,但加性效应、贡献率并不相同,显示该区间来自C堡的片段能够增加株高、穗数和百粒重从而增加产量。通过条件方法在第3染色体长臂区间RM7097-RM448及第6染色体长臂区间RM162-RM5753上定位到的产量QTL增加籽粒产量的等位基因可以降低株高,缩短生育期。 相似文献