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中国美利奴羊非繁殖季节同期发情及冻胚移植试 总被引：1，自引：0，他引：1
利用相同来源相同质量的冷冻胚胎，对中国美利奴羊进行了春季非繁殖季节和秋季繁殖季节的同期发情和胚胎移植试验。其中非繁殖季节2～3月同期处理受体母羊189只，发情101只，同期率53.4%，移植受体85只，受胎17只，受胎率20%。繁殖季节9～10月，同期处理受体羊205只，发情137只，同期率66.82%。移植121只，受胎67只，受胎率55.37%。结果表明，中国美利奴母羊在繁殖季节的同期率和胚胎移植的受胎率明显高于非繁殖季节，差异非常显著（P＜0.01）。 相似文献
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以杜湖杂交一代空怀母羊为试验动物,采用CIDR+PMSG+PG法进行同期发情和定时输精,研究PMSG剂量、母羊经产与否、同期发情处理季节对绵羊定时输精效果的影响,旨在为提高绵羊定时输精效果提供参考依据。结果表明:在同期发情处理过程中注射330 IU PMSG组的情期受胎率(81.5%)显著高于250 IU PMSG组(70.0%)(P<0.05);初配羊的情期受胎率(85.2%)显著高于经产羊(75.8%)(P<0.05);在秋季进行同期发情处理羊的情期受胎率(81.6%)显著高于春季组(70.7%)(P<0.05)。说明PMSG剂量、母羊经产与否,以及同期发情处理的季节,均对绵羊定时输精效果具有显著影响。 相似文献
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中国美利奴羊非繁殖季节同期发情及冻胚移植试验 总被引：1，自引：0，他引：1
利用相同来源相同质量的冷冻胚胎,对中国美利奴羊进行了春季非繁殖季节和秋季繁殖季节的同期发情和胚胎移植试验。其中非繁殖季节２～３月同期处理受体母羊１８９只。发情１０１只,同期率５３．４％,移植受体８５只,受胎１７只,受胎率２０％。繁殖季节９～１０月,同期处理受体羊２０５只,发情１３７只,同期率６６．８２％。移植１２１只,受胎６７只,受胎率５５．３７％。结果表明,中国美利奴母羊在繁殖季节的同期率和胚胎 相似文献
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中国美利奴羊非繁殖季节同期发情冻胚移植试验 总被引：5，自引：0，他引：5
利用相同来源相同质量的冷冻胚胎，对中国美利奴羊进行了春季非繁殖季节和秋季繁殖季节的同期发情和胚胎移植的对比试验。其中非繁殖季节2－3月份，同期处理受体母羊189只，发情101只，同期率53．4％，移植受体85只，受胎17只，受胎率20％。繁殖季节9－10月份，同期处理受体羊205只，发情137只，同期率66．82％。移植121只，受胎67只，受胎率55．37％。由试验结果看出，中国美利奴母羊在繁殖季节的同期率和胚胎移植的受胎率明显高于非繁殖季节（P＜0．01）。 相似文献
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《中国草食动物科学》2021,(3)
为了比较两种不同材质孕酮阴道栓(海绵栓和硅胶栓)对湖羊同期发情效果的影响,以便为肉羊集约化和工厂化生产提供参考,试验以997只健康、发情周期正常、无繁殖疾病的湖羊为研究对象,分两组进行同期发情处理,使用海绵栓(FGA)+PMSG处理羊551只,硅胶栓(CIDR)+PMSG处理羊446只。结果表明:FGA组处理羊只发情率为92.91%,CIDR为92.63%;FGA组处理羊受胎率为78.20%,CIDR为80.76%。发情率和受胎率两处理间差异均不显著(P0.05)。FGA组处理羊只丢栓率显著低于CIDR组3.34个百分点(P0.05);而母羊阴道发炎率显著高于CIDR组3.45个百分点(P0.05)。海绵栓单只发情成本比硅胶栓低4元。两种阴道栓各有优缺点,生产中可结合现实情况选择应用。 相似文献
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绵羊腹腔内窥镜子宫角深部冷冻精液输精试验 总被引：1，自引：0，他引：1
采用人用黄体酮，ＰＭＳＧ对绵羊进行同期发情处理，４６只绵羊４８ｈ同期发情率为９５．６５％，用冷冻精液经解冻后，活力仅０．２，借助腹腔内窥镜子宫角深部输精，情期受胎率达到７２．７３％，与澳大利亚Ｓａｌａｍａｎ教授腹腔内窥镜子宫角冷冻精液输精情期受胎率７４．０４％，仅差１．３１个百分点（Ｐ＞０．０５），且其解冻后活力为０．５；与常规冷冻精液输精的情期受胎率５５．４５％相比高出１７．２８个百分点（Ｐ＜０．０１）。 相似文献
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《新疆畜牧业》2018,(7)
新疆北部地区四季明显,大部分地方绵羊品种表现为季节性繁殖。开展高频繁殖要解决的关键就是解决非繁殖季节发情率、配种受胎率的问题。用CIDA阴道埋置12～14d撤栓,撤栓同时肌注PMSG 330IU/只。3个品种类型,发情率最高的是杜泊×小尾寒羊杂交母羊,达到88.1%;次之是萨福克×杜寒杂三元杂交母羊,为86.7%;第三是萨福克×细毛羊F3,为85.2%。发情后12～18h腹腔内窥镜子宫角冻精配种,受胎繁殖率最高的是杜泊×小尾寒羊杂交母羊,达到178.5%;萨福克×杜寒杂三元杂交母羊和萨福克×细毛羊F3,为138.1%和138.4%。羔羊初生重:以萨福克×细毛羊F3母羊羔羊初生重最高,达到3.33kg/只;杜泊×小尾寒羊和萨福克×杜寒杂生产的羔羊初生重分别为2.76kg/只和2.8kg/只。绵羊的冻精配种,利用腹腔内窥镜子宫角输精受胎率得到了极大地提高。 相似文献
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运用阴道海绵栓＋PMSG法对夏季多浪羊进行同期发情处理,并用萨福克羊颗粒冻精对同期发情羊进行定时输精。结果表明：颗粒冻精输精受胎率24.52%,颗粒冻精干、湿两种解冻法对受胎率没有显著影响。 相似文献
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为了研究季节对杜泊羊体内胚胎生产效率及胚胎移植妊娠率的影响,于2016年选取内蒙古赛诺草原羊业有限公司种羊场经产纯种杜泊母羊作为供体,采用同期发情、超数排卵及腹腔镜人工输精等方法对供体羊进行处理后,利用手术冲胚的方法获得纯种杜泊羊胚胎,同时对受体羊进行同期发情处理和胚胎移植。经统计分析,2016年全年该公司共有放栓供体4 241只,超排处理4 180只,配种供体4 131只,冲胚供体3 987只,供体可用率为94.01%;冲出胚胎总数为23 516枚,可用胚胎18 002枚,胚胎可利用率为76.55%,平均每只供体能获得可用胚胎4.52枚。供体在10月、11月、12月冲胚所获得的平均冲胚数和平均可用胚数明显高于4月、5月、6月(P<0.05),其中10月的平均冲胚数和平均可用胚数最高(P<0.01),分别为7.04枚和5.62枚。将获得的部分胚胎用于胚胎移植,移植单胚怀孕受体数为4 446只,妊娠率为53.67%;移植双胚怀孕受体数805只,妊娠率65.02%。此外,受体在1月、3月、6月、12月受胎率较其他月份高(P<0.05)。该研究可为杜泊种羊超数排卵、胚胎移植和肉羊的产业化生产提供一定的数据参考。 相似文献
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连续重复超数排卵冲胚对引进品种肉羊快速扩繁的效果研究 《畜牧与饲料科学》2022,43(2):61-68
[目的] 评价引进品种肉羊的连续重复超数排卵（超排）、冲胚、胚胎移植等繁育技术集成模式的应用效果。[方法] 于2016—2018年,对选取的1 575只澳洲白（n=230）、白头杜泊（n=228）、黑头杜泊（n=541）、白头萨福克（n=200）、黑头萨福克（n=376）5个品种母羊,进行间隔30 d以上的连续同期发情（孕酮海绵栓）与超排（330~450 IU FSH+200~300 IU PMSG+0.1 mg PG）处理;经过腹腔内窥镜输精后进行子宫角冲胚,回收胚胎后计数并评估质量,挑取部分胚胎进行移植;对比分析品种、季节、重复超排次数等对胚胎生产指标的影响。[结果] 共获得18 496枚胚胎,有14 971枚可用胚;胚胎移植后的受胎率为69.8%,产羔率为93.2%;只均总胚数和只均可用胚数2项指标显示,胚胎生产特性以白头萨福克、黑头萨福克、黑头杜泊较好,澳洲白次之,白头杜泊最差;不同品种母羊连续重复超排冲胚后回收的只均总胚数和只均可用胚数持续增长,直到第4或第5次;澳洲白的超排冲胚效果在春季较好,而其他品种在冬季较好。[结论] 连续重复超数排卵、冲胚技术对于扩繁引进肉羊品种群体是可行的,应根据不同品种及其表现出的季节特性科学、适时应用。 相似文献
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《中国畜牧杂志》2020,(3)
为评价FecB突变在鲁中肉羊培育中的应用效果,本研究首先利用TaqMan探针高通量分型技术对2295只鲁中肉羊FecB基因进行分型,统计FecB基因在鲁中肉羊群体中的分布情况,然后结合253只母羊的产羔数据和865只初生羔羊、83只3月龄母羔羊的体尺体重数据分析FecB基因对鲁中肉羊产羔数和早期生长发育的影响。群体遗传学分析表明:鲁中肉羊群体存在AA(++)、AG(B+)和GG(BB)3种FecB基因型,在新品种培育过程中突变纯合子频率逐年增加,且表现为中度多态性(0.25<多态信息含量<0.5);卡方适合性检验发现,FecB突变位点向Hardy-Weinberg平衡状态(P>0.05)转变;BB型母羊平均产羔数高于++型(P<0.01);BB型初生羔羊的体重低于++型(P<0.01),BB型初生公羔的体高、胸围、胸宽、胸深和初生母羔的胸深和管围低于++型(P<0.05),B+型3月龄母羔的管围低于++型(P<0.05)。结果显示,FecB突变显著提高了鲁中肉羊的产羔数,但对初生羔羊的生长发育存在一定的逆增长效应。 相似文献
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The fertility of 646 ewes and gimmers bred by laparoscopic artificial insemination (LAI) in the autumn of 2006 was investigated using a questionnaire and individual ewe breeding records kept for 13 commercial sheep flocks that used LAI routinely. Overall, the pregnancy rate was 66 per cent, but it was highest in ewes bred for the fourth time. Technical aspects of LAI influenced fertility: pregnancy rates were 70 per cent for ewes bred using frozen semen compared with 58 per cent when fresh semen was used (P≤0.01), and 74 per cent of ewes that travelled to an artificial insemination centre for mating conceived, compared with 62 per cent that remained on their own farm (P<0.01). Higher doses of equine chorionic gonadotrophin (>400 iu) used for oestrus synchronisation reduced pregnancy rates to only 49 per cent (P<0.001). However, the largest effect was associated with shepherds gathering, handling and treating breeding ewes four to six weeks before mating; pregnancy rates were 54 per cent among ewes where this was carried out, compared with 74 per cent for ewes not treated in this way (P<0.01). 相似文献
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冷冻精液输精深度和时间对蒙古羊受胎率的影响 《畜牧与饲料科学》2021,42(5):108-111
[目的] 研究冷冻精液输精深度和时间对蒙古羊受胎率的影响。[方法] 选取体况健康、繁殖机能正常的蒙古羊母羊作为试验动物,采用孕酮海绵栓对受体母羊进行同期发情处理。应用常规人工输精法（n=135）、过子宫颈输精法[过子宫颈第一皱褶（1~2 cm）输精（n=120）,过子宫颈第二到第三皱褶（3~4 cm）输精（n=120）]、腹腔镜辅助子宫内输精法（n=128）对发情后的母羊进行冷冻精液人工授精。接受常规人工输精法和过子宫颈输精法处理的受体母羊在发情后约14 h和22 h各输精1次,接受腹腔镜辅助子宫内输精法处理的受体母羊在发情后30~36 h输精1次。输精后40 d用B超仪检测母羊妊娠情况,计算并比较接受不同输精方法处理母羊的受胎率。[结果] 腹腔镜辅助子宫内输精法的受体母羊受胎率平均值最高,为60.2%;其次为过子宫颈第二到第三皱褶（3~4 cm）输精,受胎率平均值为58.3%;过子宫颈第一皱褶（1~2 cm）输精和常规输精法的受体母羊受胎率平均值分别为37.5%和36.3%。统计学分析表明,腹腔镜辅助子宫内输精法和过子宫颈第二到第三皱褶（3~4 cm）输精的受体母羊受胎率平均值显著（P<0.05）高于常规输精法和过子宫颈第一皱褶（1~2 cm）输精。[结论] 蒙古羊母羊发情后30~36 h采用腹腔镜辅助子宫内输精法完全穿刺子宫颈输精1次,可获得比常规输精法更高的受胎率;采用过子宫颈输精法在母羊发情后约14 h和22 h各输精1次,当授精深度接近4 cm时可达到与腹腔镜辅助子宫内输精法接近的受胎率。 相似文献
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Reproductive performance of totally confined sheep bred with semen extended in a lactose-egg yolk-glycerol buffer and stored at 5 degrees C. 
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下载免费PDF全文 Two experiments were carried out in January and May 1975 to study reproductive performance (fertility, prolificacy and fecundity) of totally confined sheep synchronized for estrus. The main objective of the first experiment was to compare reproductive performance of ewes bred naturally to those bred by artificial insemination. Semen for artificial insemination was collected by either artificial vagina or electroejaculation and stored for up to 36 hours at 5 degrees C. A second objective was to evaluate the effect of pregnant mares' serum gonadotrophin on the reproductive performance of ewes synchronized for estrus. Fertility was 53% for ewes bred by natural mating, 34% for ewes receiving pregnant mares' serum gonadotrophin and bred by artificial insemination, and 9% for those not receiving pregnant mares' serum gonadotrophin and bred by artificial insemination. There was no difference in fertility between ewes bred with semen collected by artificial vagina or by electroejaculation. A similar pattern was observed for fecundity. Average prolificacy was 2.0 with no significant differences among mating methods or pregnant mares' serum gonadotrophin application being observed. In the second experiment, the reproductive performance of ewes inseminated with semen stores at 5 degrees C for 48 to 60 hours was compared to that of ewes inseminated with semen stores for 24 to 36 hours. No significant differences in the reproductive performance were detected. Although average fertility (9%) and fecundity (19%) were low, prolificacy averaged 2.0 lambs per ewe lambing. 相似文献
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The administration of melatonin via intravaginal sponges is an effective method of advancing the breeding season in ewes. In the present study the fertility of melatonin-treated ewes has been compared with that of ewes induced to ovulate by conventional treatment. On June 25, 15, 15-month-old ewes (group 1) were given intravaginal implants containing melatonin in silastic tubing. On August 8, 13 similar ewes (group 2) were given Veramix sponges which were removed 12 days later, when they were given 500 iu pregnant mare's serum gonadotrophin (PMSG). Two intact raddled rams were introduced to the combined groups on August 21. The mean date of mating was September 3 +/- 1.5 for group 1 and August 21 +/- 0.2 for group 2 ewes. All the ewes in group 1 and 10 in group 2 (77 per cent) were mated. All the ewes were slaughtered approximately 50 days after mating and their reproductive tracts removed. The mean ovulation rates were 2.1 and 2.3 in groups 1 and 2, respectively. The results indicate that conception rates of 87 per cent and 61.5 per cent of ewes put to the ram were obtained in the melatonin-treated and PMSG-treated groups, respectively. At slaughter the melatonin-treated group were found to have a mean of 1.47 live fetuses per ewe put to the ram and the PMSG-treated group a mean of 1.08. It can therefore be concluded that melatonin implantation is an effective method for the advancement of seasonal breeding in anoestrous sheep, and that the fertility achieved is at least as good as that given by conventional progestogen-PMSG treatment. 相似文献
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专门化肉用种羊选定、引进后，通过适应性研究，在较大规模养羊业生产实践中。对现代集约化肉羊业的主要关键技术进行研究、完善和创新，组装集成并应用于生产。技术研究应用结果既为市场提供了大批专门化肉用种羊和优质安全的杂种肉羊，取得了高水平的研究成果及显著的经济效益和社会效益．又为我国目前正在迅速发展的集约化肉羊业提供了可靠的技术支持和理想的生产模式：从适宜的专门化肉羊品种到运用高效母羊快速扩繁技术．建立和完善有效的肉羊杂交利用体系，普遍应用种羊鲜、冻精生产大批肉用杂种羔羊，种植高产优质饲草及其科学加工调制和利用．建立严格兽医防疫制度．实行放牧 补饲或全舍饲的精细管理和集约化育肥，至生产优质、安全、标准肉羊，获得显著的经济效益和社会效益。 相似文献
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The effect of GnRH administration on superovulatory response of ewes treated with equine chorionic gonadotrophin (eCG) in
breeding and nonbreeding seasons and the contribution of laparoscopic insemination to the improvement of fertilization and
embryo recovery were investigated. Twenty-four nonpregnant Awassi ewes of 3–4 years of age were randomly allocated into two
groups (n = 12). Each ewe was treated with a progesterone impregnated intravaginal sponge for 12 days. The following superovulation
treatment was used: ewes of group 1 received 1,200 IU of eCG once as an intramuscular injection 48 h prior to sponge withdrawal;
ewes of group 2 also received 1,200 IU of eCG once as an intramuscular injection, 48 h prior to sponge withdrawal and after
24 h of sponge removal. Ewes were injected with 80 μg of GnRH. Ewes of groups 1 and 2 were further subdivided into four equal
groups (n = 6). Subgroups A and C (superovulated with eCG and eCG plus GnRH, respectively) were mated naturally at least two times
with Awassi rams of proven fertility at 8-h intervals. Subgroups B and D (same as A and C) had intrauterine insemination at
44–46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of diluted semen containing
100 × 106 motile sperm in the distal portion of each uterine horn. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy at day 6 after mating. Embryo recovery was performed by using a semi-laparoscopic flushing procedure in both
uterine horns. Results of the present study showed that ewes treated in breeding season with eCG plus GnRH has a higher number
(P < 0.05) of corpora lutea than eCG alone as 7.33 ± 0.54 and 4.33 ± 0.39, respectively. There was no significant difference in the number of corpora lutea in nonbreeding season when ewes treated with eCG and eCG plus GnRH. The number of unovulated follicles was significantly
higher (P < 0.05) in eCG treated ewes than in ewes treated with eCG plus GnRH, both in the breeding and nonbreeding seasons. The number
of recovered embryos from ewes treated with eCG plus GnRH and eCG differ significantly (P < 0.05) as 4.32 ± 0.56 and 1.06 ± 0.26, respectively, in the breeding seasons. No significant difference was observed when
these hormones used for superovulation in the nonbreeding season. A higher number of unfertilized ova (P < 0.05) was observed in ewes when naturally inseminated than in ewes inseminated using the intrauterine laparoscopic technique.
Higher rate of embryo recovery (P < 0.05) was achieved when ewes were inseminated via intrauterine (4.66 ± 0.66) compared with ewes naturally mated (2.16 ± 0.74).
The fertilization rate in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 91.5% and 44.8%,
respectively. Fertilization failure in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were
8.4% and 55.2%, respectively. It could be concluded that administration of GnRH 24 h after sponge removal increased ovulation
rate of Awassi ewes treated with eCG for superovulation in the breeding season. The use of eCG to induce superovulation in
Awassi ewes combined with laparoscopic intrauterine insemination increases the fertilization rate. 相似文献