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1、猪囊尾蚴病又名囊虫病．是绦虫的幼虫——囊尾蚴（Cysticercus）人畜共忠的一种寄生虫疾病。虫体为乳白色．体扁平．呈带状．形如大米，由600-1000个节片构成．节片分为头节．颈节、未成熟节成熟节和孕卵节。每个孕卵节片内含约3万-5万个虫卵孕卵节片时常数节同时脱离虫体．孕卵节片破裂后虫卵散布污染在自然环境中．在适宜的环境中可生存数周，能感染猪、牛、羊、犬、骆驼及人体．是人畜共患的疾病． 相似文献
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从江及都匀地区牛带绦虫试验感染猪牛后从囊尾蚴寄生部位鉴别虫种 总被引：1，自引：0，他引：1
目的 观察从江及都匀牛带绦虫感染牛、猪的寄生部位分布及其形态鉴别虫种。方法 取贵州从江县及都匀市牛带绦虫病人虫体的3节孕节(约30000～40000虫卵)直接喂养14日龄约克种乳猪2头和17日龄荷兰乳牛犊2头，于62日龄、67日龄剖杀，观察囊尾蚴分布、形态、测量大小．观察成熟情况。结果 都匀牛带绦虫试验感染猪、牛，其囊尾蚴寄生在肝脏。从江牛带绦虫试验感染牛，其囊尾蚴寄生在全身肌肉，感染猪则仅寄生在肝脏。从江及都匀牛带绦虫感染猪牛后，两者囊尾蚴寄生部位不同。都匀牛带绦虫感染的猪囊尾蚴原头节有两圈类似小钩的点状结构，而从江牛带绦虫感染猪后原头节无小钩。结论 根据从江及都匀牛带绦虫感染牛、猪后囊尾蚴的寄生部位及其原头节小钩的差异，可以证实都匀牛带绦虫是亚洲牛带绦虫。 相似文献
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宋辉 《畜牧兽医科技信息》2019,(9)
<正>猪囊尾蚴病是一种世界性分布的人畜共患病寄生虫病。猪囊尾病是由猪带绦虫,链状带绦虫的中绦期幼虫猪囊尾蚴寄生于人、猪、犬、猫的肌肉、成虫寄生在人的小肠而引起的。感染者是主要传染源,通过排出的孕节和虫卵感染易感对象。本病的易感者是人和猪,包括野猪,人对猪囊尾蚴普遍易感。人是终末宿主,自然条件下是猪带绦虫的唯 相似文献
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猪带绦虫有成虫、虫卵、六钩蚴、囊尾蚴不同形式的发育阶段和寄生状态，不同的虫体形式以独特的组织结构和生命活动规律满足其寄生在相应宿主体内的需要。研究发现，六钩蚴与囊尾蚴在虫体组成成分、生化指标以及代谢(分泌)产物等方面都有差异。SDS-PAGE和免疫印迹研究表明，六钩蚴的抗原成分非常复杂，不仅有共同抗原、阶段发育特异性抗原，而且在入侵宿主定植在肌肉组织后，表 相似文献
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本病由数种绦虫引起,常呈地方性流行。它不仅可使犊牛发育不良,而且可引起牛死亡。作者就多年基层工作经验,对该病的中医治疗做一论述,供大家参考。1 病原引起牛绦虫病的病原寄生虫有莫尼茨绦虫、曲子宫绦虫及无卵黄腺绦虫。其中莫尼茨绦虫危害最严重。虫体的共同特征为黄白色带状,由头节、颈节和许多体节组成长带状,长的可达5米,最长的可达10米。头颈细小,体节和原卵节较宽大,为雄雌同体,卵或脱落的节片随粪便排出体外,排出的节片因蠕动蜷曲形似麦粒状。孕卵节和虫卵随宿主粪便排出体外,被中间宿主吞食后,发育成侵袭性的似囊尾蚴。牛、羊误食带有似囊尾蚴的土壤螨而被感染。侵袭性虫卵经牛、羊消化道卵膜被消化而释放出似囊尾蚴,它吸附在小肠黏膜上,逐渐发育为成虫,成虫生活期限为2~6个月。 相似文献
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《畜牧兽医科技信息》2020,(6)
正细颈囊尾蚴病是寄生在狗、狼、狐小肠内的泡状带绦虫的幼虫—细颈囊尾蚴寄生在猪、反刍动物和野生动物等(中间宿主)的肝脏或肠系膜、浆膜、网膜等处所引起的寄生虫疾病。此病分布范围很广,在世界各地均有细颈囊尾蚴病的存在。本文详细的分析了细颈囊尾蚴病的发病机制以及病状,并给出了有效的预防措施。1病原泡状带绦虫(成虫)主要寄生在狗、狼和狐的小肠内,是一种大型绦虫,其成虫呈白色而微黄,虫体由250～300个节 相似文献
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为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。 相似文献
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绵羊肉孢子虫可溶性蛋白抗原的特异性初探 总被引：1，自引：1，他引：0
应用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS—PAGE)分析绵羊肉孢子虫囊壁和不同种肉孢子虫滋养体蛋白带,结果表明:肌肉中消化分离出的羊犬肉孢子虫滋养体和从食道壁上挑出的巨肉孢子虫滋养体均出现三条电泳蛋白带;巨肉孢子虫全虫体可溶性蛋白出现五条蛋白带;巨肉孢子虫囊壁可溶性蛋白出现两条电泳蛋白带;微小住肉孢子虫滋养体可溶性蛋白出现一条电泳蛋白带。用不同种的滋养体可溶性蛋白抗原和巨肉孢子虫囊壁抗原做IHA试验表明:用羊犬肉孢子虫和巨肉孢子虫滋养体可溶性蛋 相似文献
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异源性抗原抗猪囊尾蚴感染的研究 总被引：1，自引：0，他引：1
本文报道了泡状带绦虫活化六钩蚴的超声裂解抗原可以诱导猪体产生抗猪带绦虫攻击感染的交叉保护作用。猪囊尾蚴匀浆抗原也使猪体产生了较强的抗猪带绦虫攻击感染的保护作用。泡状带绦虫六钩蚴超裂抗原免疫组与猪囊尾蚴匀浆抗原免疫组的保护情况是相似的,这表明异源免疫也可使猪体产生较好的抗猪囊尾蚴感染的免疫。由于制备异源性抗原的泡状带绦虫能够从狗的体内获得,因此在体外培养猪带绦虫未获成功之前可以解决从人体获取猪带绦虫的困难。 相似文献
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Serodiagnosis of porcine cysticercosis by enzyme-linked immunosorbent assay (ELISA) using fractionated antigens 总被引：1，自引：0，他引：1
The sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Taenia solium cysticercosis was evaluated in experimentally and naturally infected pigs, using T. solium larval scoleces and its fractionated 1st and 2nd peaks on Sephadex G-200 as antigens. First peak antigen gave maximum sensitivity and highest antibody titres. The overall sensitivity of this test was found to be 91.5, 95.8 and 70.8% with scolex, 1st and 2nd peak antigens, respectively. False positive reactions occurred in 9.09% of uninfected pigs with scolex and 1st peak antigens and cross-reactions occurred in 25% of Taenia hydatigena-infected animals using scolex and 2nd peak antigens. No cross-reaction was observed using 1st peak antigen. The specificity of the test was 92.3, 96.2 and 92.3% with scolex, 1st and 2nd peak antigens, respectively. 相似文献
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本研究旨在观察豆状囊尾蚴在不同状态下的超微结构。从肉联厂屠宰检验采取豆状囊尾蚴,直接剪破囊泡从中取出豆状囊尾蚴;或用含10%胆汁生理盐水培养囊尾蚴,待其从囊泡中翻出后,用扫描电镜观察和记录。位于囊泡内的头节,从其顶端观察,顶突呈伞状,皮肌柱连接小钩覆盖于头节的前端。从侧面观察,一排有鹿角样的小钩附着于顶突。4个吸盘呈洞穴状位于顶突之后分布在头节的四周。原始颈节较宽,体节较细,表面均有许多皱褶。培养后的豆状囊尾蚴可明显区分为头节、颈节、体节和囊泡区,顶突上的皮肌柱收缩,鹿角样的小钩向周围伸展,吸盘也发生环形和纵形收缩,颈节的环状收缩和体节的纵行收缩均明显可辨。豆状囊尾蚴处于不同状态时,其超微结构明显不同。 相似文献
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An unknown gene, SLC10, was cloned by spliced leader-based polymerase chain reaction from Taenia solium. The full length of SLC10 was found to be 635 bp, encoding an 18.223 kDa protein. ELISA results showed that none of 70 normal and 75 cysticercosis sera samples reacted with purified recombinant SLC10 protein. Using an immunohistochemical method, it was revealed that the native SLC10 protein distributed extensively in inner cyst walls but not in the scolex in Cysticercus cellulosae. Together with predicted results, it is suggested that the SLC10 protein is a non-secretory structural protein, which is not involved in induction of the host's immune reactions against infection at least at the larval stage. 相似文献
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Studies were conducted to evaluate antigens of Taenia solium (Tso) and Taenia crassiceps (Tcra) cysticerci in the ELISA test for the diagnosis of swine cysticercosis. The samples analyzed were cysticercosis positive and negative control sera and heterologous sera. Four antigens were assayed: vesicular fluid (VF) and crude (T) Tcra and scolex (S) and crude (T) Tso. All antigens showed good performance, but VF-Tcra was the best followed by T-Tcra. Sensitivity rates of ELISA were respectively, in 2nd and 3rd standard deviation cut-offs, 96.0 and 80.0% for the VF antigen and specificity of 97.5 and 100.0%. Cross-reactivity was verified only for hidatidosis and ascaridiosis. Due to the high performance observed, the ELISA test using Tcra antigens should be recommended for the diagnosis of swine cysticercosis. 相似文献
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Specific antibody responses to Taenia hydatigena, Taenia pisiformis and Echinococcus granulosus infection in dogs 总被引：1，自引：0，他引：1
Groups of dogs reared free of both nematodes and cestodes were infected with Taenia hydatigena, Taenia pisiformis or Echinococcus granulosus. After infections with the Taenia spp became patent, dogs were purged to remove the worms. They were later reinfected and the second infections again removed by purging after patency. A group of 3 uninfected worm free dogs was kept as age-matched controls. The dogs were bled at intervals of 5 days and their serums tested for antibodies using the enzyme-linked immunosorbent assay (ELISA) with excretory/secretory (ES) antigens collected during in vitro incubation of evaginated scoleces (scolex ES antigen) and oncosphere antigens. Antibodies to scolex ES antigen were detected by 3 weeks after infection with each cestode species whereas antibodies to oncosphere antigen were not detected until about one week after eggs were found in the faeces of the infected dogs. Antibody responses to both oncosphere and scolex ES antigens decreased rapidly following removal of the worms by purging. Uninfected control dogs were invariably negative to both oncospheral and scolex ES antigens. There were cross-reactions between the serums from dogs infected with T. pisiformis and T. hydatigena when tested with scolex ES antigens, but oncospheral antigens showed a high degree of species specificity. Scolex ES antigens from E. granulosus were compared with those prepared from T. hydatigena and T. pisiformis for their ability to discriminate between antibodies in serums collected from dogs 31 and 32 days after infection with 100,000 protoscoleces of E. granulosus or dogs infected with Taenia spp.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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兔豆状囊尾蚴的压片技术及染色方法研究 总被引：1，自引：0，他引：1
为了详细观察豆状囊尾蚴头节的形态结构,通过屠宰检验或病兔剖检采集的豆状囊尾蚴的囊泡,制作压片后用不同的染色方法进行了比较.结果表明,制作豆状囊尾蚴头节压片的关键是载玻片要洁净、干燥,从囊泡中取出的头节应完整无损,并尽量除去黏附在头节上的囊液.将带有头节的结节放在载玻片的中部,再在其上放一载玻片,两手在载玻片的两端均匀用力挤压,待结节全部展开即可.为了观察头节的正面结构,在压制玻片前,应用手术刀片除去原始颈节与体节部分.通过常用的几种单染色和复染色法对压片进行染色,证明单染色更有利于详细的观察头节的结构,而且几种单染色之间有互补染色效果. 相似文献
19.
Lightowlers MW Colebrook AL Gauci CG Gauci SM Kyngdon CT Monkhouse JL Vallejo Rodriquez C Read AJ Rolfe RA Sato C 《Veterinary parasitology》2003,115(2):83-123
Highly effective recombinant vaccines have been developed against the helminth parasites Taenia ovis, Taenia saginata and Echinococcus granulosus. These vaccines indicate that it is possible to achieve a reliable, high level of protection against a complex metazoan parasite using defined recombinant antigens. However, the effectiveness of the vaccines against the taeniid cestodes stands in contrast to the more limited successes which characterise attempts to develop vaccines against other platyhelminth or nematode parasites. This review examines the features of the host-parasite relationships among the taeniid cestodes which have formed the basis for vaccine development. Particular consideration is given to the methodologies that have been used in making the cestode vaccines that might be of interest to researchers working on vaccination against other helminths. In developing the cestode vaccines, antigens from the parasites' infective larval stage contained within the egg (oncosphere) were identified as having the potential to induce high levels of protection in vaccinated hosts. A series of vaccination trials with antigen fractions, and associated immunological analyses, identified individual protective antigens or fractions. These were cloned from cDNA and the recombinant proteins expressed in Escherichia coli. This strategy was independently successful in developing vaccines against T. ovis and E. granulosus. Identification of protective antigens for these species enabled rapid identification, cloning and expression of their homologues in related species and thereby the development of effective vaccines against T. saginata, E. multilocularis and, more recently, T. solium. The T. saginata vaccine provides an excellent example of the use of two antigen components, each of which were not protective when used individually, but when combined they induce a reliable, high level of protection. One important contributing factor to the success of vaccine development for the taeniid cestodes was the concentration on studies seeking to identify native host-protective antigens, before the adoption of recombinant methodologies. The cestode vaccines are being developed towards practical (commercial) application. The high level of efficacy of the vaccines against T. solium cysticercosis and hydatid disease suggests that they would be effective also if used directly in humans. 相似文献
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猪带绦虫45W基因已被认为是预防猪囊虫病的基因工程疫苗候选基因之一。其中4B基因是45W基因家族中高度保守的一个成员（以下称45W-4B）。本试验将重组于pGEM-3Z载体上的4B基因转载到pVL1393转移载体中，通过鉴定获得重组质粒pVL1393-4B，然后将重组质粒与BmNPV病毒共转染，筛选出重组BmNPV-4B重组病毒，用该重组病毒感染Bm细胞，收集细胞上清，SDS-PAGE电泳鉴定表达蛋白，并经Western blot检测表明该蛋白能识别囊虫病人（猪）阳性血清，45W-4B蛋白的成功表达对进一步研究45W蛋白的结构和功能以及开发高效猪囊虫基因工程疫苗打下了基础。 相似文献