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猪瘟、猪丹毒、猪肺疫三联活疫苗保存期试验黄保松广西壮族自治区生物制品厂南宁５３０００１在我厂历年的产品留样中，随机抽取于－１５℃条件下保存１年１０个月至５年４个月的猪瘟、猪丹毒、猪肺疫三联活疫苗的样品共８批，按９２版《规程》［１］进行猪瘟、猪丹毒、猪... 相似文献
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王洪图 《中国预防兽医学报》1989,(Z1)
我厂生产猪瘟、猪丹毒、猪肺疫三联苗(以下简称猪三联苗)36批,有9批因冻干后菌数低而报废,占投产批次的25％。笔者就猪瘟牛睾丸细胞苗(以下简称猪瘟苗)是否对猪丹毒、猪肺疫菌有抑制作用进行了试验,现报告如下。材料与方法一、猪瘟苗:本厂猪瘟组生产。二、猪丹毒(G_4T_(10))、猪肺疫(E_0630)二联菌液的制备:将冻干保存的猪丹毒、猪 相似文献
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《养殖与饲料．饲料世界》2003,(1):39-39
疫苗名称预防的疫病)干J量猪瘟免化弱毒冻干活疫苗猪肺疫弱毒冻f话菌苗猪丹;续弱,价东卜活菌苗猪瘟猪丹毒猪肺疫_二联活疫苗l一d头份/头1头份/头l头份/头lm{/失·次猪J」毒猪肺疫二联了舌疫苗猪副伤寒弱毒冻十话菌苗伪百l友亨、材灭分舌苗(浓)乙少亏。月户l炎弱毒冻l字子了,交苗细小才l芍,{工火活油f左剂l、,不猪气J喘挤j勺弱毒冻]一了严、一有“;,1仃猪大肠们菌K、、于、双价;浅{I、}{一私‘苗猪大肠小}一菌火活!钩苗仃猪红痢火话{有苗传染州胸膜肺炎灭活油仁剂J’l’l猪链球菌板板化招l%l苗不浓)猪瘟猪肺疫猪丹毒猪瘟猪肺疫、猪丹毒猪… 相似文献
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为建立马丁琼脂培养基质量控制标准,系统评价马丁琼脂培养基适应性和促生长能力,运用多杀性巴氏杆菌、丹毒杆菌和链球菌类活疫苗活菌计数参考品,通过活菌计数统计分析,对新鲜马丁琼脂、干粉马丁琼脂或改良马丁琼脂进行适应性和促生长能力系统测试。结果显示：新鲜马丁琼脂、干粉马丁琼脂和改良马丁琼脂均对多杀性巴氏杆菌、丹毒杆菌、链球菌的适应性和促生长能力差异显著,需同时选择巴氏杆菌类、丹毒杆菌类、链球菌类活疫苗计数参考品评价马丁琼脂培养基的适应性和促生长能力,表明活菌计数参考品用于建立马丁琼脂质量控制标准是可行的。 相似文献
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猪瘟是猪的一种严重传染病,对养猪业的危害非常大,因此在每年的春、秋两季防疫中,猪瘟被列为首防的疫病。目前,使用的疫苗主要有猪瘟活疫苗（Ⅱ）和猪三联苗（猪瘟、猪丹毒、猪肺疫三联活疫苗）,均为“中牧”公司生产。猪三联苗可以同时预防对应的三种疫病,节约时间,因此被广大防疫员使用。现就如何正确合理使用猪瘟疫苗及猪三联苗谈一点肤浅的看法。 相似文献
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猪瘟是猪的一种严重传染病,对养猪业的危害非常巨大,因此在每年的春、秋两季防疫中,猪瘟被列为首防的疫病。目前,我们使用的疫苗主要有猪瘟活疫苗（Ⅱ）和猪三联苗（猪瘟、猪丹毒、猪肺疫三联活疫苗）,均为“中牧”公司生产。猪三联苗可以同时预防对应的三种疫病,节约时问,因此很多防疫员喜欢使用。现就如何合理使用猪瘟疫苗及猪三联苗谈一点肤浅的看法。 相似文献
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章国祥 《江西畜牧兽医杂志》1983,(4)
<正> 兽医科研部门虽然研制出猪瘟、猪丹毒,猪肺疫三联苗,但由于三联苗在生产工艺上要求高,要进行批量生产还有一些困难,另外各地猪的疫情不同,注射联苗又会造成浪费和一次接种疫(菌)苗种类过多影响免疫效果,因此,目前我省生产的仍然是单价疫(菌)苗。根据我县猪的疫情情况,每年春、秋二季开展大面积的预防注射,一猪要打三至四针,兽医和防疫员的劳动强度很大,工效不高,群众又不欢喜。为减轻基层兽医和防疫员在预防注射工作中的劳动强度,提高工效,消灭和控制猪瘟、猪丹毒、猪链球菌病,自一九八一年秋防以来在全县普遍推行了用 相似文献
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为便于猪多杀性巴氏杆菌病活疫苗（E0630株）质量控制和降低生产成本,研制了ZJ-1和ZJ-2两种合成培养基,并进行了两种合成培养基与马丁肉汤的比较试验。结果表明,合成培养基ZJ-1培养活菌数高于ZJ-2和马丁肉汤。用合成培养基ZJ-1培养制备3批疫苗,并分别用兔进行安全检验和小鼠效力检验。3批疫苗对兔均2／2健活,对小鼠的保护力分别为9／10、9110、8／10,符合产品质量标准。本试验表明,运用合成培养基替代传统培养基是可行的。 相似文献
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1株猪源病原菌的分离鉴定及部分生物学特性研究 总被引：4，自引：0，他引：4
通过对一起病死猪的实验室诊断 ,从病猪心血、脏器中分离到了 1株对小白鼠和家兔都具有较强致病性的细菌。经显微镜观察、培养特性观察、生化特征鉴定 ,此菌为多杀性巴氏杆菌 ,但此菌与其他多杀性巴氏杆菌不同的是它能利用水杨素、鼠李糖、阿拉伯糖。 O抗原琼脂扩散试验鉴定此菌菌体抗原为 O1型 ;Carter间接血凝试验法检验荚膜抗原类型此菌为 A型。体外滤纸扩散法检验此巴氏杆菌对药物敏感性 ,试验表明该菌耐青霉素、土霉素、四环素、磺胺嘧啶、痢特灵、阿米卡星、头孢唑啉 ;但对庆大霉素、卡那霉素、盐酸环丙沙星、氟哌酸敏感 ,对氨苄西林 ,链霉素中度敏感。自制灭活苗进行免疫原性实验检测 ,本分离菌疫苗对小白鼠和家兔保护率约为 70 % ,而市售巴氏杆菌疫苗对小白鼠和家兔保护率约为 3 0 %～ 40 %。 相似文献
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Immunity assays on sheep sera using passive mouse protection tests showed that vaccines containing more than 4 strains of Pasteurella multocida did not give a good immunity. The immune response was not enhanced by the use of an oil adjuvant, and high concentrations of bacteria had only a partial positive effect. Attempts to extract selectively the protection-inducing antigen(s) from P. multocida by veronal, phenol or potassium thiocyanate extraction were unsuccessful. Furthermore, it was found that sheep antisera to the recognized type strains of P. multocida afforded only limited protection against a number of field strains. We concluded from this that successful immunization against ovine pasteurellosis will depend on either the identification of a strain of P. multocida that gives a wide spectrum of immunity or the discovery of a live mutant suitable for vaccine production and the definition of cultural conditions that promote the expression of a common immunizing antigen. 相似文献
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将研制的马丁干粉培养基与新鲜马丁培养基比较,证明用干粉培养基培养链球菌、多杀性巴氏杆菌和丹毒杆菌,其每1 mL中活菌数和灵敏度均与新鲜马丁培养基相当.干粉培养基用于生物制品的生产及检验更方便且质量稳定,可推广应用. 相似文献
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通过将兔多杀性巴氏杆菌C51—17株菌液分装后置-80℃冻存的方法对菌液浓度进行预数,并与传统方法采用的将菌液置4℃保存过夜计算菌存率或通过测定菌液吸光值推算攻毒菌液浓度相比较。结果表明。用-80℃冻存保存方法估算的菌液浓度与实际浓度更接近,采用这种方法进行疫苗的效力检验,能确保对照成立,在兔多杀性巴氏杆菌病灭活疫苗效力检验中可以采用-80℃冻存的方法进行攻毒菌液的预数。 相似文献
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Purification of a cross-protective antigen from Pasteurella multocida grown in vitro and in vivo 总被引：1，自引：0，他引：1
Rimler RB 《Avian diseases》2001,45(3):572-580
A peptone-based medium was formulated to grow Pasteurella multocida in vitro, which expressed an antigen that induces cross protection in turkeys against different serotypes. Vaccines of various chromatographic fractions obtained from P. multocida grown in the medium induced active immune cross protection in turkeys, and sera from these turkeys passively cross protected na?ve poults. An antigen of approximately 39 kD molecular size was purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution from hydroxyapatite chromatographic fractions of both in vivo- and in vitro-grown P. multocida. The purified antigen from either source induced active immune cross protection but no passive protection in one of two experiments. Increasing the dose of vaccine resulted in both active and passive immune cross protection in the second experiment. 相似文献
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Investigation was done to determine the presence of Pasteurella multocida and related species in free ranging chickens and ducks, dogs, cats and pigs in three climatic zones (cool, warm and hot) of rural Morogoro, Tanzania. A total of 153 isolates of P. multocida ssp. multocida and related species were obtained by direct culture on blood agar, selective medium and mouse inoculation. P. multocida ssp. multocida was isolated from 0.7% of chickens and 7% of ducks. In dogs and cats, P. multocida ssp. multocida was isolated from 1 and 68%, respectively. One isolate of Pasteurella gallinarum was isolated from a duck. Other species obtained were; P. multocida ssp. septica, Pasteurella stomatis and taxon 16 from dogs and cats, while Pasteurella dagmatis and Pasteurella canis were found in dogs only. Prevalence of P. multocida ssp. multocida was significantly higher (P<0.01) in ducks of the warm zone (22%) than in ducks of other zones (0%). No significant difference was observed between the prevalence of P. multocida ssp. multocida in chickens of the warm zone (2%) and chickens of the cool and hot zones (0%). Extended phenotypic characterization revealed phenotypic similarities between two isolates from chickens and the duck strains. Mouse inoculation appeared to be more sensitive in detecting P. multocida ssp. multocida than blood agar and selective medium. Direct culture on blood agar recovered most of the isolates from dogs. This study has demonstrated for the first time the presence of P. multocida and related species in the village free ranging chickens, ducks, dogs and cats in Tanzania. Other non-classified Pasteurella spp. were also observed in the study, but further characterization is required before the final classification can be made. This paper reports for the first time the isolation of unclassified Pasteurella from dogs and cats in Africa. The results implies that fowl cholera might be occurring in free ranging poultry, and dogs and cats kept in contact might serve as sources of P. multocida to chickens and ducks. Subsequent applications of molecular techniques to analyse the epidemiological relatedness of clones isolated from different host species is indicated. 相似文献
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对妊娠期母猪进行猪瘟疫苗免疫,观察其抗体变化情况;采用5种商品猪瘟疫苗免疫仔猪,检测其在一免和二免情况下的抗体变化规律.结果表明：仔猪猪瘟母源抗体与母猪的抗体水平正相关,妊娠母猪在妊娠期60-80 d免疫无显著差异,不同来源疫苗的免疫效果相似,二次免疫效果优于一次免疫. 相似文献
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2018年9月广西某羊场部分山羊发生流涕、咳嗽、呼吸困难和体温升高等临床症状的疾病,为确诊发病原因并提供治疗方案,采用病原分离培养、PCR扩增鉴定的方法进行诊断,并对分离菌进行生化鉴定、致病性试验和药敏试验。结果显示,病料在血平板有圆形的小菌落生长,革兰阴性小球短杆菌,而在PPLO培养基上不生长;病料的PCR扩增结果显示绵羊肺炎支原体和多杀性巴氏杆菌均为阳性;所分离到的病原菌经生化鉴定,该菌符合多杀性巴氏杆菌的特性;用多杀性巴氏杆菌种属和D型多杀性巴氏杆菌特异性引物扩增为阳性;致病性试验显示该菌对小鼠有很强的致病性;药敏试验显示该菌对头孢他啶、头孢噻肟、氧氟沙星高度敏感,对复方新诺明、强力霉素、红霉素、青霉素为耐药。结果表明该病是由绵羊肺炎支原体和D型多杀性巴氏杆菌混合感染引起。 相似文献