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应用3批鸡传染性鼻炎二价油乳剂灭活疫苗进行了免疫持续期的试验。结果表明，鸡群免疫1次后，对A型强致病力菌株的攻毒保护率为83%，对C型强致病力菌株的攻毒保护率为51.5%，在首免后4周左右进行二免，则免疫鸡群对A型菌的保护率在免疫后13个月仍然保持100%，对C型菌的保护率在免疫后9个月内可达到90.5%。免疫鸡血清抗体也均保持较高的水平，其中B-ELISA的A型抗体的检出率为83%-100%、C型的检出率为33%-87%，血凝抑制试验（HI）的A型抗体阳性检出率为87%-100%，C型抗体的检出率为62.8%-93%，血清平板凝集抗体的检出率为62.8%-100%，此结果充分显示了鸡传染性鼻炎二价油乳剂灭活疫苗的良好的免疫效力以及B-ELISA、HI及血清平板凝集试验较高的敏感性的特异性，研究还证实了HI抗体与攻毒保抗力的密切相关。 相似文献
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为了观察EDS-76油乳剂灭活苗的最佳保存期,用4批EDS-76油乳剂灭活苗（批号0701、0702、0703、0704）分别置0～4℃、11～20℃（室温）,经保存0、1、2…369、12个月后,检查其物理性状,并按1个免疫剂量免疫EDS-76阴性的120日龄的伊莎褐母鸡,免疫后30日检测血清中HI抗体。结果表明,这4批灭活苗于0～4℃和11～20℃分别保存12个月和3个月,物理性状不变;O～4℃保存9个月和12个月HI抗体有所下降,仍能达到较高的抗体滴度水平,起到免疫保护作用;在11～20℃保存3个月时HI抗体,下降到7．9Log2左右,但仍远远高于保护水平;因此,本疫苗保存期为0～4℃暂定为1年,11～20℃暂定为3个月。 相似文献
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为观察四元材料制备的EDS-76油乳剂灭活苗的最佳保存期,用4批EDS-76油乳剂灭活苗(批号 200001、200002、200003、200004),分别置0～4 ℃、11～20 ℃(室温),经保存0、1、3、6、8、9、12个月后,检查其物理性状,并按1个月免疫剂量免疫EDS-76阴性的120日龄的伊莎褐母鸡,免疫后25日检测血清中HI抗体.结果表明,这批苗于0～4 ℃和11～20 ℃分别保存12个月和8个月,物理性状不变;4℃保存9个月和12个月HI抗体有所下降,仍能达到较高的抗体滴度水平,起到免疫保护作用;在11～20 ℃保存3个月时HI抗体下降到8Log2左右,但仍远远高于保护水平;因此,本疫苗保存期4℃暂定为1 a,11～20 ℃暂定3个月. 相似文献
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本研究对国内市场上几个主要鸡传染性鼻炎灭活疫苗生产厂家生产的灭活疫苗免疫鸡只进行血清HI抗体水平测定和攻毒,比较不同公司所产疫苗效力的效力差异,并评估A、C型二价灭活疫苗两次免疫鸡群对国内B型分离株:DL-1株和最近从免疫失败鸡场分离到的SD-1株的交叉保护作用,分析免疫失败的原因.结果表明:A、D、E公司的疫苗免疫两次后,A型HI抗体阳性率分别为92.5%、100%和95%,滴度分别为33.9、55.1和59.9,攻毒保护率都是100%.C型HI抗体阳性率分别为72.5%、38.5%和77.5%,滴度分别为11.4、2.7和27,攻毒保护率分别为80%、70%和80%.而B和C公司的疫苗免疫两次后A型HI抗体阳性率分别为59.4%、77.1%,抗体滴度分别为5.4和21.8,攻毒保护率分别为50%和66.7%;C型HI抗体阳性率分别为54.1%和51.4%,抗体滴度分别为6.1和6.8,攻毒保护率分别为38.3%和50%.在五个疫苗产品中,以A、D、E的保护效力较好,B、C产品效力较差.另外,A、C型二价灭活疫苗免疫后不能对B型菌的攻击提供保护,其A、C型HI抗体阳性率、抗体滴度与对B型菌攻毒保护率无相关性. 相似文献
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为了观察ND-GS O1油乳剂灭活疫苗的最佳保存期,用四批ND油乳剂灭活疫苗(批号为0701、0702、0703、0704)分别置4~8℃冰箱、11~20℃室温,经保存0、1、2、3、4、6个月后,检查其物理性状,并按1个免疫剂量免疫ND阴性的45日龄乳鸽,免疫后21日检测血清中HI抗体。结果表明,这四批疫苗于4~8℃和11~20℃分别保存6个月和3个月,物理性状不变。4~8℃保存6个月HI抗体有所下降,但仍能达到较高的抗体滴度水平,起到免疫保护作用。在11~20℃保存3个月HI抗体下降到7.3 log2左右,但仍高于保护水平。因此,本疫苗保存期4~8℃暂定为6个月,11~20℃暂定为3个月。 相似文献
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为了观察四元材料制备的ND油乳剂灭活疫苗的最佳保存期,用4批ND油乳剂灭活疫苗（批号为0701、0702、0703、0704）分别置4～8℃冰箱、11～20℃室温,经保存0、1、2、3、4、6个月后,检查其物理性状,并按1个免疫剂量免疫ND阴性的30日龄乳鸽,免疫后21日检测血清中HI抗体。结果表明,这4批疫苗于4～8℃和11～20℃分别保存6个月和3个月,物理性状不变。4～8℃保存6个月HI抗体有所下降,但仍能达到较高的抗体滴度水平,起到免疫保护作用。在11～20℃保存3个月HI抗体下降到7.4log2左右,但仍远远高于保护水平。因此,本疫苗保存期4～8℃暂定为6个月,11～20℃暂定为3个月。 相似文献
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为了观察四元材料制备的ND蜂胶佐荆灭活疫苗的最佳保存期,用四批ND蜂胶佐剂灭活疫苗(批号为 0601、0602、0603、0604)分别置4～8 ℃冰箱、11～20 ℃室温,经保存0、1、2、3、4、6个月后,检查其物理性状,并按1个免疫剂量免疫ND阴性的30日龄乳鸽,免疫后14 d检测血清中HI抗体.结果表明,这四批疫苗于4～8 ℃和11～20℃分别保存6个月和3个月,物理性状不变.4～8 ℃保存6个月HI抗体有所下降,但仍能达到较高的抗体滴度水平,起到免疫保护作用.在11～20 ℃保存3个月HI抗体下降到7.510g2左右,但仍远远高于保护水平.因此,本疫苗保存期4～8 ℃暂定为6个月,11～20 ℃暂定为3个月. 相似文献
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利用Page A型、B型、C型副鸡禽杆菌和新城疫病毒La Sota株,研制鸡传染性鼻炎(三价)和新城疫二联油乳剂灭活疫苗。用3个批次疫苗进行单剂量(0.5mL/次)3次接种和大剂量(2.0mL)单次接种的安全性试验、SPF鸡的免疫效力试验、商品鸡的免疫持续期试验和疫苗的保存期试验。结果表明,3批疫苗对试验鸡安全无副作用;免疫接种SPF鸡只30d后对A、B、C型副鸡禽杆菌攻毒的保护率≥80%,用20μL/只疫苗免疫接种SPF鸡21d后新城疫的平均HI抗体24;商品鸡42日龄首免,110日龄二免,二免后9个月对A、B、C型副鸡禽杆菌攻毒的保护率≥70%,对新城疫病毒强毒100%保护;用4℃~8℃保存12个月和15个月的3批疫苗进行了SPF鸡的近期免疫效力试验,结果对A、B、C型副鸡禽杆菌攻毒的保护率≥70%,用20μL/只疫苗免疫SPF鸡,免疫接种后21d新城疫病毒的平均HI抗体24,对新城疫病毒强毒的攻毒保护率70%。说明研制的疫苗安全有效。 相似文献
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鸡传染性支气管炎病毒血凝抑制试验抗原的研制 总被引：1，自引：0，他引：1
为应用血凝抑制(HI)试验检验鸡传染性支气管炎疫苗免疫效力(血清、卵黄抗体水平),建立了鸡传染性支气管炎病毒HI试验抗原的制备方法。该方法是通过选取抗原谱最广的鸡传染性支气管炎病毒(IBV)M41株,经SPF鸡胚增殖培养36h后无菌收取鸡胚尿囊液,4℃、12 000r/min离心10min,上清用聚乙二醇(PEG)20000浓缩100倍;兔源A型产气荚膜梭菌中国标准株(C57-1株)37℃增殖培养18h,4℃、12 000r/min离心10min取上清,经PEG 20000透析袋浓缩5倍后通过0.20μm滤膜过滤除菌,然后将IBV液和A型产气荚膜梭菌菌液(含α毒素)二者按一定比例混合后经37℃恒温振荡感作2h,4℃经48h后制成。经大量试验表明,制备的IBV HI试验抗原效价高、稳定性好,可替代进口抗原应用于鸡群IBV疫苗免疫后血清抗体及卵黄抗体的HI效价检测。 相似文献
11.
The aims of this study were the identification, cloning, and expression of a genetic region encoding an epitope that induces hemagglutination inhibition (HI) antibody against Avibacterium paragallinarum serovar A and an evaluation of the recombinant protein for immunogenicity in chickens. Although two monoclonal antibodies (MAbs) with HI activity, designated S24-951 and S7-1716-5C, were generated in this study, no reactive proteins with both MAbs were identified by Western blot analysis. A gene fragment of 5157 bp, designated hpa5. 1, was cloned from genomic DNA, and a recombinant protein expressed by hpa5.1, designated HPA5.1, reacted with both MAbs on dot-blot analysis. HPA5.1 showed no hemagglutinating activity, but significantly absorbed HI antibodies in the chicken immune serum. Analysis using a series of deletion mutants prepared from hpa5.1 indicated that a 4.8 kbp gene in hpa5.1 is essential for the expression epitope recognized by MAb S24-951. In addition, chickens immunized once with HPA5.1 showed a high protection rate with sufficient HI antibody titers against challenge exposure with a virulent strain of A. paragallinarum serovar A strain 221. These results show that hpa5. I1 is responsible for the expression of an epitope that induces HI antibody, and HPA5.1 might be a candidate for the development of a new vaccine against avian infectious coryza caused by A. paragallinarum serovar A. 相似文献
12.
为了监测鸡新城疫、传染性支气管炎、禽流感(H9亚型)三联灭活疫苗(LaSota株+M41株+SS/94株)对H9亚型禽流感病毒流行毒株的免疫保护效果,采用H9亚型禽流感病毒SS/94株及2009—2010年现地分离的3株H9亚型禽流感病毒对已免疫上述三联灭活苗的SPF鸡进行攻毒试验。结果显示,试验鸡以0.3 mL/只的剂量免疫三联灭活苗后21 d,其H9亚型禽流感病毒的HI抗体效价可达8～11log2,此抗体水平可抵抗2×106EID50的H9亚型禽流感病毒SS/94株、BLCN09株、WDZ09株、YT10株的攻击,攻毒保护率均达90%(9/10)以上。可见,以SS/94株作为禽流感疫苗抗原制备的三联灭活苗具有良好的免疫原性,能使免疫鸡抵抗2009—2010年期间现地分离的多株H9亚型禽流感病毒的攻击。 相似文献
13.
The efficacy of experimental inactivated infectious coryza vaccines produced by a commercial vaccine manufacturer was evaluated. The vaccines, containing as the adjuvant phase either a double-emulsion mineral oil system or aluminum-hydroxide gel, were administered to 6-week-old chickens as a single dose. Some vaccines were a monovalent product containing a Page serovar C Haemophilus paragallinarum strain, and others were a bivalent product containing both Page serovar A and serovar C strains. After 3 weeks, all chickens were challenged by infraorbital sinus inoculation of virulent H. paragallinarum, either Page serovar C (strain HP31) or Page serovar A (strain HP14). The monovalent serovar C double-emulsion-based vaccines gave significant protection against a serovar C challenge, with the level of protection varying from 60% to 100%. The monovalent serovar C aluminum-hydroxide-gel vaccine also gave significant protection (94%) against a serovar C challenge. The bivalent double-emulsion vaccine gave significant protection against challenge from both serovars (100% for serovar C and 83% for serovar A). Although no major adverse reactions were detected, some chickens receiving both the double-emulsion vaccines and the aluminum-hydroxide vaccine developed relatively minor granulomatous reactions at the site of injection. 相似文献
14.
Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA 总被引：1，自引：0，他引：1
Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as the immunogen for vaccination. The rHagA subunit vaccine protected 71% of immunized chickens against 10(10) colony-forming units (CFU) of viable A9. Vaccinated chickens which showed no clinical signs of coryza developed haemagglutination inhibition (HI) titers of 1:10 or greater. Haemagglutination (HA) of serovars A and C was not affected by the presence of rHagA specific antiserum. The HA of rHagA could only be induced against formaldehyde-fixed chicken red blood cells (FA-RBCs). These results suggested that HagA is a moderate immunogen and might not be a major haemagglutinin in vivo. However, HagA might be involved in haemagglutination when treated serovar C aggregates fixed RBCs in vitro. 相似文献
15.
Wenyan Shi Qing Liu Jie Zhang Jingjing Sun Xiyue Jiang Jing Geng Fangkun Wang Yihong Xiao Hongmei Li Xiaomin Zhao 《Research in veterinary science》2014
In the present study, a naked EtMIC2 DNA vaccine, a ChIL-18 expression vector and a EtMIC2 and ChIL-18 co-expression DNA vaccine were constructed and their protective efficacies against homologous challenge were compared and evaluated by examining the body weight gain, oocyst shedding, cecal lesion, ACI as well as specific anti-EtMic2 antibody level, the proliferation ability and percentages of CD4+ and CD8+ of splenocytes. The results showed the naked EtMIC2 DNA vaccine could increase the weight gain and decrease the oocyst shedding, but could not alleviate the cecal lesion of immunized chickens compared to unimmunized chickens. Chickens immunized with the co-expression vector pVAX1-MIC2-IL-18 exhibited much improved immune protection against challenge compared to chickens immunized with naked EtMIC2 DNA vaccine, or with naked EtMIC2 DNA vaccine and ChIL-18 expression vector applied separately. These results suggest that the co-expression of ChIL-18 with EtMic2 together could significantly improve the immune protection of the EtMic2 protein. 相似文献
16.
对应用当地分离病毒株研制的鸡产蛋下降综合症系列油乳剂灭活苗进行了免疫试验。结果表明,免疫后,鸡产蛋下降综合症（ＥＤＳ７６）油乳剂灭活苗、鸡新城疫（ＮＤ）－产蛋下降综合症二联油乳剂灭活苗及鸡新城疫－鸡传染性支气管炎（ＩＢ）－产蛋下降综合症三联油乳剂灭活苗免疫组的血清ＥＤＳ７６ＨＩ抗体迅速上升,维持４个月后仍在６．８－８（ｌｏｇ２）以上,并且能够抵抗强毒的攻击。证明所研制的ＥＤＳ７６油苗、ＮＤ－ＥＤＳ７６二联油苗、ＮＤ－ＩＢ－ＥＤＳ７６三联油苗对ＥＤＳ７６具有良好的免疫作用,能够抵抗ＥＤＳ７６强毒的攻击并产生高而持久的血清ＨＩ抗体。此外,对ＮＤ－ＥＤＳ７６二联苗、ＮＤ－ＩＢ－ＥＤＳ７６三联苗免疫组的血清ＮＤＨＩ抗体测定结果表明,上述二联苗、三联苗能产生与接种ＮＤ油苗一样良好的ＮＤ免疫效果,在免疫后１３０天时,其血清ＨＩ抗体仍高达９－１１（ｌｏｇ２）以上,与对照组有极显著的差异 相似文献
17.
A serological classification of 106 Japanese isolates of Haemophilus paragalinarum recovered from 1960 to 1984 was performed by dot-blotting and hemagglutination-inhibition (HI) tests using two serovar-specific monoclonal antibodies (MAbs), E5C12D10 and F2E6. By the dot-blotting test, 49 of the isolates were serovar A and 55 isolates were serovar C, and the two remaining isolates did not react with either MAb. These two nontypable strains had no hemagglutinating activity against chicken erythrocytes and were nonpathogenic to chickens. Although 49 serovar A isolates were serotyped by the HI test, only 23 of the 55 serovar C isolates could be serotyped. The remaining 32 isolates could not be serotyped because no or low hemagglutinating activity could be detected. Our results indicate that H. paragallinarum serovars A and C have both been present in Japan since 1960, with serovar A isolates being dominant before 1970 and serovar C isolates more prevalent than serovar A since 1970. 相似文献
18.
In general, avian influenza (AI) vaccines protect chickens from morbidity and mortality and reduce, but do not completely prevent, replication of wild AI viruses in the respiratory and intestinal tracts of vaccinated chickens. Therefore, surveillance programs based on serological testing must be developed to differentiate vaccinated flocks infected with wild strains of AI virus from noninfected vaccinated flocks in order to evaluate the success of vaccination in a control program and allow continuation of national and international commerce of poultry and poultry products. In this study, chickens were immunized with a commercial recombinant fowlpox virus vaccine containing an H5 hemagglutinin gene from A/turkey/Ireland/83 (H5N8) avian influenza (AI) virus (rFP-H5) and evaluated for correlation of immunological response by hemagglutination inhibition (HI) or agar gel immunodiffusion (AGID) tests and determination of protection following challenge with a high pathogenicity AI (HPAI) virus. In two different trials, chickens immunized with the rFP-H5 vaccine did not develop AGID antibodies because the vaccine lacks AI nucleoprotein and matrix genes, but 0%-100% had HI antibodies, depending on the AI virus strain used in the HI test, the HI antigen inactivation procedure, and whether the birds had been preimmunized against fowlpox virus. The most consistent and highest HI titers were observed when using A/turkey/Ireland/83 (H5N8) HPAI virus strain as the beta-propiolactone (BPL)-inactivated HI test antigen, which matched the hemagglutinin gene insert in the rFP-H5 vaccine. In addition, higher HI titers were observed if ether or a combination of ether and BPL-inactivated virus was used in place of the BPL-inactivated virus. The rFP-H5 vaccinated chickens survived HPAI challenge and antibodies were detected by both AGID and HI tests. In conclusion, we demonstrated that the rFP-H5 vaccine allowed easy serological differentiation of infected from noninfected birds in vaccinated populations of chickens when using standard AGID and HI tests. 相似文献
19.
Wambura PN 《Tropical animal health and production》2009,41(2):149-152
The efficacy of green-coloured (GC) I-2 Newcastle disease vaccine was determined in the present study. I-2 vaccine was mixed
with a green coloured dye and stored at 4°C for 6 months while assayed for the virus infectivity at a monthly interval. Chickens
were vaccinated with the GC vaccine by eye drop. Serum samples were collected from all birds before and after vaccination
at weekly interval for 4 weeks and tested for haemagglutination-inhibition (HI) antibody against Newcastle disease virus (NDV). These chickens were challenged with NDV virulent strain four weeks after vaccination. The results showed that there
was no difference between the infectivity titres of GC and uncoloured vaccines. However, chickens vaccinated with GC vaccine
produced higher HI antibody titres than chickens vaccinated with uncoloured vaccine. Results from the challenge trial showed
that all vaccinated chickens survived whereas all unvaccinated chickens died. The findings from this study have shown that
the GC vaccine is safe and produced protective antibodies against NDV in vaccinated chickens.
Wambura, P. N., 2008. Protective antibody response produced by the chickens vaccinated with green coloured thermostable Newcastle
disease virus. Tropical Animal Health and Production. 相似文献