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不同培养基成分对铁皮石斛组织培养的影响 总被引：5，自引：1，他引：4
以铁皮石斛无菌苗的茎段作为外植体,讨论MS培养基中添加不同浓度的激素对铁皮石斛的诱导、增殖、分化以及生根的影响。结果表明：最适合铁皮石斛原球茎诱导的培养基为MS+ 6-BA 0.5 mg/L+ NAA 1.5 mg/L,原球茎诱导率为95%;最适合原球茎增殖的培养基为MS+ 6-BA 1 mg/L+ NAA 1 mg/L;最适合原球茎分化的培养基为MS+ 6-BA 5 mg/L+ NAA 1 mg/L,其分化率达80%;最适合铁皮石斛根诱导的培养基为MS+IBA 1.5 mg/L+香蕉泥100 g/L,其生根率为100%。 相似文献
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铁皮石斛组织培养及快繁技术研究 总被引：2，自引：0，他引：2
为建立铁皮石斛快繁技术体系,给生产提供参考,研究了铁皮石斛组织培养过程中的灭菌方法、腋芽诱导、原球茎的诱导、增殖与分化的最佳培养基配方。以铁皮石斛成熟茎段为外植体,探讨1/2MS培养基或MS培养基中添加不同浓度的激素对铁皮石斛组织培养过程中各生长、分化阶段的影响。研究表明,腋芽诱导最适宜培养基的配方为MS+ 6-BA 2.0 mg/L+ NAA 0.5 mg/L,诱导率达91.28%,平均芽数达2.34;原球茎诱导的最适宜培养基为1/2MS+ 6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L,诱导率可达66.67%;原球茎增殖最佳培养基为1/2MS+ 6-BA 3.0 mg/L+NAA 0.3 mg/L,原球茎分化最适宜培养基为1/2MS+ NAA 1.0 mg/L+ 6-BA 3.0 mg/L+ KT 1.0 mg/L,最适宜生根的培养基配方为1/2MS+NAA 2.0 mg/L+ AC 0.5 g/L。 相似文献
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以条叶唇柱苣苔(Chirita ophiopogoidesDFang etW T Wang)的幼嫩叶片、种子为外植体,以MS为基本培养基,建立了条叶唇柱苣苔的组织培养和再生体系.试验结果表明:条叶唇柱苣苔叶片诱导不定芽的最佳培养基为MS +2,4-D0.5 mg/L+ IAA 0.5 mg/L+ NAA 0.2 mg/L,丛生芽分化的最佳培养基为MS+ KT 1.0 mg/L+ IAA 3.0 mg/L+ NAA 0.5mg/L; MS+ NAA 0.5 mg/L+ 6-BA 1.5 mg/L的培养基最适合继代,MS+ IBA 2.0 mg/L+ AC 0.5 mg/L培养基的生根效果最好,平均根长为4.67 cm,生根率达100％. 相似文献
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以新疆杨叶片为外植体,研究新疆杨组织培养再生体系建立的主要影响因素。结果表明:诱导愈伤组织的最佳培养基组合为MS+0.5 mg/L 6-BA+0.5 mg/L 2,4-D;愈伤组织分化的最佳培养基组合为MS+0.1 mg/L TDZ+0.3 mg/L IBA,不定芽增殖的最佳培养基组合为MS+1.0 mg/L 6-BA+0.3 mg/L IBA,诱导生根的最佳培养基组合为1/2MS+0.5 mg/L IBA,新疆杨组培苗在生根培养基上形成的根条数多,苗体健壮,生根率达到100%。本研究成功建立了新疆杨组织培养再生体系,为其转基因研究工作奠定了基础。 相似文献
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以肇东紫花苜蓿和Pleven6紫花苜蓿的子叶、下胚轴为外植体,建立离体再生体系。结果如下：最佳愈伤诱导培养基：肇东苜蓿为MS+2.0mg/L2,4-D+0.5mg/ＬKT+3%蔗糖,Pleven6为MS+1.5mg/L2,4-D+0.5mg/LKT+3%蔗糖;分化培养基：MS+2.0mg/L6-BA+0.5mg/L NAA+3%蔗糖;分化继代培养基：MS+0.3mg/L6-BA+0.1mg/LNAA+0.3mg/LKT+3%蔗糖;生根培养基：1/2MS+0.5mg/LNAA+1.5%蔗糖。两个品种的子叶均较下胚轴分化时间短、分化效率高,肇东苜蓿诱导率和分化率明显高于pleven6苜蓿。 相似文献
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介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
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DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
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香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。 相似文献
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基于RS与GIS技术,利用景观生态学理论及景观格局指数对布尔津县土地利用现状进行定量分析。结果显示为：（1）布尔津县以草地、林地为主要景观类型,镶嵌着耕地、水域等景观类型。（2）四个分区（即高山区、丘陵区、平原区、荒漠区）的林地资源组成差异明显,耕地、居民、交通及工矿用地集中分布在丘陵区和平原区（3）从各项指数来看,高山区的多样性指数较低;平原区是受人文因素最多的区域,景观优势度低,各种土地利用类型比例差异不大,有利于生态可持续发展,但要合理的开发利用;荒漠区生态环境相对脆弱,要加强生态环境的保护与改善。通过该研究对布尔津县的土地利用现状作一个了解,为该县乃至整个地区的景观规划和可持续发展战略提供科学依据。 相似文献
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Based on the relation between the incipient yield pressure and the limit of yielding in cylinder according to the elastic disabled rule, a laboratorial method of determining limit of yielding of material is designed and a set of experimental data were gained by the method. The incipient yield pressure of the cylinder is gained by analyzing the experimental data, and then the limit of yielding is gained. The laboratorial method has a good reliability as the experimental value has a little error to the theory value. It has a definite engineering signification and theory value to research the mechanical capability of material and the disabled rule of high pressure vessel. 相似文献
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南瓜降糖功能及其系列食品加工技术 总被引：3，自引：0，他引：3
阐述了南瓜在糖尿病辅助治疗方面的功能特性,介绍了南瓜降糖主要制品南瓜全粉、南瓜脆片、南瓜果酱和南瓜肉汁的工艺流程和操作要点。 相似文献
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母猪繁殖障碍成因分析 总被引：3，自引：0，他引：3
繁殖障碍是导致母猪淘汰的最主要因素,直接造成母猪非生产天数延长,繁殖猪群生产效率降低,严重影响猪场的经济效益。本文作者对引起母猪繁殖障碍的多方面因素,包括营养因素（如饲养水平、饲料品质、断奶日龄）、环境因素（如温度、湿度、通风、光照）、疫病因素、生产技术及管理因素进行了详细阐述。 相似文献
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Efficiency of water use of early plantings of sunflower 总被引：4，自引：0，他引：4
M. Auxiliadora Soriano Francisco Orgaz Francisco J. Villalobos Elías Fereres 《European Journal of Agronomy》2004,21(4):465-476
Rain fed crop production in Mediterranean environments depends to a large extent on strategies that avoid the intense summer drought. Early plantings of sunflower have given consistently higher yields in such environments, but the basis for such yield increases has not been explored. We conducted two field experiments at Cordoba (Spain) to investigate the effects of an early and a late planting date on the components of water-limited crop productivity; namely, water use (T), water use efficiency (TE) and harvest index (HI) of sunflower. The results were generalized by simulating rain fed sunflower yields, under early (1 January) and late (15 March) plantings, for a 25-year period with the aid of a simulation model of the Ceres type (OILCROP-SUN) which has been validated in Cordoba. Experimental seed yields of early plantings in 1989 and 1996 were 2.0 and 3.0 t ha−1, while late plantings yields were 1.3 and 2.4 t ha−1, for the 2 years. Average simulated yields were 2.7 ± 1.1 and 1.9 ± 0.7 t ha−1 for early and late plantings, respectively. For the 2 years, T of early plantings was higher than that of late plantings, but the response of TE and HI to planting date was not the same in the two experiments. In the simulation exercise, T and TE of early plantings were consistently higher than those of late plantings, while there were no differences in the HI for the two planting dates. We conclude that early plantings of sunflower increase rain fed yields by increasing both T and TE, while the impact of planting date on HI very much depends on the crop water stress pattern, which is quite variable from year to year even in the predictable Mediterranean environment. 相似文献