共查询到17条相似文献，搜索用时 328 毫秒
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[目的]选择不同品种牛,利用同期发情和自然发情后作为受体牛,进行日本和牛冻胚移植研究。[方法]选择奶牛20头和南德温杂种肉牛41头作为受体牛,利用CIDR+PG做人工同期发情处理,发情后的受体牛移植和牛冻胚。统计分析受体牛的同期发情率和移植后的妊娠率。[结果]试验结果表明奶牛和南德温杂种肉牛的受体牛同期发情率分别为90.00%和85.36%,差异显著(P<0.05);在同一牛场场使用不同品种受体牛进行冻胚移植试验结果表明,15头奶牛和27头南德温杂种肉牛受体牛妊娠率分别为46.67%和48.14%,差异不显著(P>0.05)。[结论]只要符合胚胎移植受体牛要求,奶牛和南德温杂种肉牛均可作为和牛胚胎移植受体牛。 相似文献
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对绵羊胚胎移植过程中,受体不同发情方式、受体不同卵巢状态对妊娠率的影响进行研究,结果表明:在受体不同发情方式,同期发情组鲜胚妊娠率为59.46%,自然发情组鲜胚妊娠率为50.00%,两组无显著差异(P>0.05);在冻胚移植中,发情组妊娠率为46.22%,未发情组为16.00%,在妊娠率上两组差异极显著(P<0.01);其妊娠率发情组为46.22%,二情期返情组为25.88%,两组差异极显著(P<0.01)。有一个黄体的受体羊,妊娠率38.37%,有多个黄体的受体羊,妊娠率37.93%,2组差异不显著(P>0.05);有卵泡受体羊,妊娠率29.55%,无卵泡受体羊妊娠率40.51%,两组妊娠率差异不显著(P>0.05)。 相似文献
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影响受体牛冷冻移植胚胎效果的主要因素 总被引：3，自引：0，他引：3
试验研究影响受体牛移植冷冻胚胎效果的主要因素,目的是解决胚胎移植产业化中容易出现的技术问题,以提高牛胚胎移植妊娠率。在季节、饲养管理、同期发情处理和胚胎移植技术以及环境条件等基本相同的情况下,结果表明:西门塔尔杂交牛的移植利用率和移植妊娠率高于黄牛杂交牛和黑白花奶牛,但各组间无显著性差异(P>0.05);2～6周岁的受体牛移植利用率和移植妊娠率高于7周岁以上和2周岁以下的受体牛,但各组间无显著性差异(P>0.05);经产1～3胎受体牛移植利用率显著高于4胎以上的受体牛(P<0.05),而移植妊娠率则无显著性差异(P>0.05);体况上等与中等的受体牛移植利用率和移植妊娠率无显著性差异(P>0.05);同期发情处理时卵巢处于黄体期的移植利用率显著高于卵泡期,差异极显著(P<0.01);左侧卵巢有黄体的子宫角胚胎移植妊娠率显著高于右侧(P<0.05);胚龄对胚胎移植妊娠率高低依次为早期囊胚、桑椹胚和囊胚,但无显著性差异(P>0.05)。 相似文献
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不同胚胎移植方法对天祝白牦牛受胎率的影响 总被引：1，自引：0，他引：1
为提高胚胎移植技术在牦牛上的运用效果,选用51头甘肃省天祝县健康黑牦牛作为受体,以纯种天祝白牦牛作为供体生产胚胎,分别对同期发情处理和自然发情的受体牛进行鲜胚和冻胚移植试验。结果,同期发情处理的受体牛鲜胚移植的受胎率显著高于冻胚移植的受胎率(P0.05),分别为52%和38.5%;在自然发情受体牛的胚胎移植中也得到了相似结果,鲜胚和冻胚的移植受胎率分别为60%和50%,同期发情处理牛的平均妊娠率则低于自然发情受体牛的平均妊娠率(P0.05),二者分别为47.5%和54.5%。结论,牦牛鲜胚移植的受胎率明显高于冻胚移植的受胎率,而且自然发情受体牛的受胎率高于同期发情处理牛。 相似文献
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《中国畜牧杂志》2017,(7)
为提高优质肉用种用绵羊胚胎移植的效率,试验选取76只黑萨福克羊和27只杜泊羊作为供体,采用"CIDR+FSH+PMSG"法进行超数排卵处理。选取384只小尾寒羊为受体,采用"CIDR+PMSG"法进行同期发情处理,以探索供体胚胎发育阶段、胚胎冷冻处理、胚胎体外停留时间以及移植给受体时移植侧黄体数量等因素对胚胎移植妊娠率的影响。结果表明:移植新鲜囊胚的妊娠率极显著高于桑椹胚(P0.01);冷冻/解冻囊胚移植妊娠率极显著低于新鲜囊胚(P0.01),而冷冻/解冻桑椹胚移植妊娠率与新鲜桑椹胚无显著差异(P0.05);此外,移植桑椹胚时,移植侧有1个黄体的受体妊娠率极显著低于有2~3个黄体受体妊娠率(P0.01),但移植囊胚时两者间差异不显著(P0.05)。综上可知,子宫角移植桑椹胚和囊胚时,移植新鲜囊胚、移植冷冻桑椹胚效率较高;将新鲜桑椹胚移植给移植侧有2~3个黄体受体效果好于1个黄体受体。 相似文献
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本试验分别采用CIDR+E2法和两次PGF2α子宫输注法,对胚胎移植受体牛做同期发情处理,鲜胚移植观察其妊娠情况。试验结果表明两种处理方法的受体牛在24~48h发情率分别为85.5%和51.6%,两者之间差异显著(P<0.05)。移植前对受体牛进行黄体检查,黄体合格率分别为79.3%和77.8%,差异不显著(P>0.05);受胎率分别为45.8%和42.4%,差异不显著(P>0.05)。24h后观察发情,6.5d直检,黄体A、B级者用于移植。 相似文献
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梅花鹿新鲜和冷冻胚胎移植技术的研究 总被引：1，自引：0，他引：1
实验对12头供体母鹿进行超数排卵,27头受体同期发情后进行胚胎移植,建立梅花鹿胚胎移植技术体系。同期发情经埋置CIDR处理,超数排卵的供体每头分8次注射总剂量320 mg的FSH。供体发情鉴定后8~12 h通过直肠把握法进行人工授精。结果表明:冲胚时的胚胎回收率为74.39%(61/82),所获胚胎中,囊胚、桑椹胚、2-细胞、未受精卵所占的比例分别为1.64%(1/61)、77.05%(49/61)和1.64%(1/61)、19.67%(12/61)。研究将20枚新鲜胚胎移植给14头受体;将28枚胚胎经细管法玻璃化冷冻、解冻后移植给13头受体。鲜胚移植的受体妊娠率为85.71%(12/14),产仔率为64.29%(9/14);冻胚移植的受体妊娠率为61.54%(8/13),产仔率为53.85%(7/13),二者没有显著性差异(P0.05)。 相似文献
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红安格斯牛超数排卵和胚胎移植受体同期发情处理 总被引：3，自引：0，他引：3
选择100头红安格斯母牛使用2种超数排卵处理方案,有效超排供体母牛77头。对受体牛采用3种方案进行同期发情处理。结果表明:育成母牛和经产母牛平均回收胚胎数分别为8.45、9.23枚(P>0.05),平均回收可用胚胎数分别为6.33、7.02枚(P>0.05);2种超排方案均取得较好的效果,超排方案1和方案2分别回收胚胎数为10.10、7.63枚,回收可用胚胎数为6.62、6.51枚。受体同期发情处理一次PG法、二次PG法及CIDR+PG法,同期发情率分别为71.19%、74.44%和77.63%(P>0.05),发情受体利用率分别为66.67%、64.93%及69.49%(P>0.05),胚胎移植妊娠率分别为50.00%、55.17%和56.10%(P>0.05),与自然发情受体利用率及胚胎移植妊娠率无显著性差异。 相似文献
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试验对绵羊胚胎移植过程中,受体不同发情方式、受体不同卵巢状态对妊娠率的影响进行了研究.结果表明,在受体不同发情方式,同期发情组鲜胚妊娠率为59.46%,自然发情组鲜胚为50.00%,在妊娠率上两组无显著差异(P＞0.05);在冻胚移植中,发情组妊娠率为46.22%,未发情组为16.00%,在妊娠率上两组差异极显著(P＜0.01);其妊娠率发情组为46.22%,二情期返情组为25.88%,在妊娠率上两组差异极显著(P＜0.01).有一个黄体受体羊,妊娠率38.37%,有多个黄体受体羊,妊娠率37.93%,两组妊娠率差异不显著(P＞0.05);有卵泡受体羊妊娠率29.55%,无卵泡受体羊妊娠率40.51%,两组妊娠率差异不显著(P＞0.05). 相似文献
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绵羊胚胎移植受胎率影响因素的研究 总被引：1，自引：0，他引：1
为研究探索绵羊胚胎移植受胎率的影响因素,针对不同体况受体、供受体发情时间差、胚胎质量、单侧卵巢黄体数量及移植胚胎数量等进行试验研究。结果表明:体况较好组、体况中等组、体况较差组受胎率分别为67.62%、63.44%、52.08%,三组之间差异不显著(P>0.05);发情同步差在0h、±6h、±12h的受胎率分别为72.73%,68.18%,66.67%,三组之间差异不显著(P>0.05);移植A级胚胎的受胎率(67.24%)高于B级胚胎的受胎率(42.86%),差异显著(P<0.05);1个黄体的受胎率为61.76%,2个黄体的受胎率比1个黄体的高出3.69%,两者之间差异不显著(P>0.05);移植两枚胚胎的受胎率(69.77%)高于移植一枚胚胎的受胎率(62.5%),但差异不显著(P>0.05)。 相似文献
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[目的]为了进一步促进人工授精技术广泛应用以及提高胚胎移植效率，为酒泉本地胚胎移植选择高效的同期发情方法。[方法]本试验分别采用PRID＋PGF2α法和PGF2α＋PGF2α法对胚胎移植受体牛做同期友情处理。[结果]表明：两种方法处理受体牛，其同期发情率和移植妊娠率之间差异无统计意义（P〉0．05），但是用PRID＋PGF2α法处理受体牛同期发情率和移植妊娠率均高于PGF2α＋PGF2α法。[结论]在奶牛养殖及生产上，在考虑移植成本和生产效益的情况下，在农区大面积对牛只进行同期发情技术处理，应优选PGF2α＋PGF2α法处理受体牛。 相似文献
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为了研究欧拉羊群在春秋两季规模化同期发情效果,利用CIDR＋PG法及两次PG法对大群体舍饲和半舍饲欧拉羊进行同期发情实验,比较不同处理方法的同期发情效果、不同季节对同期发情效果及不同饲养方式的发情效果。结果表明：采用CIDR＋PG法同期发情率为92.3%,与两次PG法同期发情率78.7%,差异显著（P〈0.05）;采用两次PG法在春季、秋季对欧拉羊同期发情处理,春季与秋季欧拉羊的0～48h同期率分别为73.3%、76.5%,差异不显著（P〉0.05）;对不同饲养方式的欧拉羊同期发情处理,舍饲养殖的欧拉羊0～48 h的同期发情率为58.0%,放牧＋舍饲同期发情率为78.9%差异显著（P〈0.05）。 相似文献
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Nishigai M 《The Journal of reproduction and development》2003,49(1):23-36
The conditions of embryo transfer by the stepwise method, in which frozen-thawed embryos are transferred on day 7 (day 0=onset of estrus), were investigated with the aim of increasing pregnancy rates in frozen-thawed embryo transfer. The use of a vaginal speculum to prevent bacterial infection when passing an embryo transfer gun through the vagina yielded a pregnancy rate equal to or higher than that with application of a sheath cover to the transfer gun. Administration of a sedative, xylazine, to recipient cattle for preventing movement at the time of embryo transfer improved the pregnancy rate. The influence of the time from thawing of frozen embryos to transfer and of the transportation of the recipient by truck upon pregnancy rate was investigated. Embryo transfer within 60 minutes after aspiration into a straw or transportation of the bovine recipient, 1.5 hours each way before and after transfer, had no influence on pregnancy rate. Relations of the embryonic developmental stage and morphological quality after thawing of frozen embryos to pregnancy rate were investigated in recipients of nulliparous Holstein heifers. The pregnancy rate increased as the embryonic developmental stage advanced from compacted morula, early blastocyst, and blastocyst in that order. The pregnancy rate obtained with blastocyst stage embryos was significantly (P<0.05) higher than that with compacted morula stage embryos, and there was no significant difference in pregnancy rates between excellent morphological quality and good morphological quality for compacted morula stage embryos. When correlation of luteal function and pregnancy rate was investigated in bovine recipients, pregnancy rate showed a tendency to increase with increasing blood progesterone (P) concentration on the day before (on day 6 after estrus) and the day of embryo transfer. The pregnancy rate in bovine recipients, which showed a blood P concentration of > or =2.5 ng/ml on the day before embryo transfer, was significantly (P<0.05) higher than that in those with a blood P concentration of <2.5 ng/ml. Pregnancy rate showed a tendency to increase with decreasing blood estradiol-17beta (E2) concentration on the day of embryo transfer. Activation of luteal function by administration of human chorionic gonadotropin (hCG) in cycling cattle was investigated for its effect on increasing pregnancy rate in bovine recipients. A follicle coexisting with cyclic CL ovulated and induced CL formed after injection of hCG 1,500 IU 5 days after ovulation. The blood P concentration was significantly (P<0.05) higher in the administration group than in the control group, and the blood E2 concentration rapidly decreased, showing a lower concentration than in the control group. These results suggest the possibility that the pregnancy rate could be improved by administration of hCG. Pregnancy rate following intramuscular injection hCG 1,500 IU was comparatively investigated in parous Japanese Black beef cattle receiving frozen-thawed embryos 7 days after estrus. Pregnancy rate was 67.5% in the group in which hCG was administered on day 6 after estrus, and was significantly (P<0.05) higher than that in the control group (45.0%) and the group in which hCG was administered on day 1 after estrus (42.5%), revealing that hCG administration facilitated pregnancy. Transfer of frozen-thawed embryos in the blastocyst stage within 60 minutes after the aspiration into a straw, with a vaginal speculum after administration of xylazine is suggested as a way of improving pregnancy rate in bovine recipients with favorable luteal function and in those with luteal function activated by administration of hCG on the day before embryo transfer. 相似文献
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奶牛超数排卵及胚胎移植的试验研究 总被引：1，自引：0，他引：1
本试验采用FSH＋PG的方法对22头供体荷斯坦牛分两批进行了超数排卵处理,140头受体南阳黄牛和供体牛采用2次PG法分批进行了同期发情处理。结果如下：（1）共有20头供体牛、104头受体牛在第二次注射PG后96h内发情,同期发情率分别为90.90%和74.29%;（2）20头供体牛回收胚胎176枚,可用胚胎140枚/头,平均回收胚胎8.8枚/头,平均可用胚胎7.0枚/头,可用胚胎比率79.55%;（3）104头南阳黄牛受体均移植单胚,51头妊娠,妊娠率为49.04%。 相似文献
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B Alexander G Coppola GF Mastromonaco E St. John ER Reyes DH Betts WA King 《Reproduction in domestic animals》2008,43(2):207-211
Early pregnancy diagnosis and monitoring play an important role following embryo transfer in sheep. The aims of the current study were to investigate (i) the pattern of serum progesterone profiles in sheep carrying somatic cell nuclear transfer (SCNT)‐derived (clone) pregnancies, and (ii) the frequency of pregnancy loss during development following SCNT embryo transfer. Sheep SCNT embryos were made using standard nuclear transfer techniques. Day 7 embryos were surgically transferred to oestrus‐synchronized recipients (n = 27). As a control, normal fertile ewes (n = 12) were bred by natural breeding. Serum was collected from all the ewes on the day of estrus (day 0 sample), 7 days post‐estrus (day 7 sample) and 19 days post‐estrus (day 19 sample) and every 10 days thereafter until lambing or pregnancy loss occurred. Serum progesterone (P4) was assessed using enzyme immunoassay. Pregnancy was confirmed by ultrasound scanning on day 35 of pregnancy followed by subsequent scanning every 10 days. In control ewes, pregnancy rate on day 35 was 83.3% (10/12), whereas in the ewes that received SCNT embryos, it was 22.2% (6/27; p < 0.05). The day 45 pregnancy rate in the control ewes was 83.3%, whereas in the SCNT embryo recipients it was 11.0% (p < 0.05). Hormone analysis revealed that SCNT embryo recipients exhibited a significantly lower P4 profiles at different time points in pregnancy compared to controls (p < 0.05). This study highlights the use of serum progesterone in combination with ultrasound for the investigation of embryo loss and crucial times during development of normal and SCNT embryos in sheep. Further, the serum P4 levels directly reflect the degree of placental development in these two groups. 相似文献
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旨在探讨以小尾寒羊和蒙古羊作为受体时,二者的胚胎移植效果及所产羔羊的早期生长性能之间的差异。选用南非肉用美利奴羊(n=11)和澳洲白绵羊(n=110)作为供体,以小尾寒羊(n=196)和蒙古羊(n=504)作为受体;对供体母羊进行超数排卵以及人工授精处理,记录供体母羊的收集胚胎总数和可用胚胎数,计算平均每只羊收集的可用胚胎数以及胚胎合格率;对小尾寒羊和蒙古羊进行同期发情及胚胎移植处理,记录受体母羊的产羔数,计算繁殖率;计算并比较不同受体母羊所产羔羊的平均初生重、成活率以及70日龄断奶羔羊数、70日龄断奶重、平均日增重、平均每只母羊提供的断奶羔羊数。结果表明,从供体母羊中共获得可用胚胎549枚,平均每只羊收集可用胚胎4.54枚,胚胎合格率为82.56%;利用胚胎移植技术,移植受体母羊529只,所产羔羊240只,平均繁殖率为45.37%;小尾寒羊的发情率和繁殖率明显高于蒙古羊;共获得70日龄断奶羔羊211只,羔羊成活率为87.92%,平均日增重为280.00g;小尾寒羊和蒙古羊所产的羔羊初生重没有显著差异(P>0.05),小尾寒羊所产羔羊的70日龄断奶重和平均日增重分别极显著(P<0.01)和显著(P<0.05)高于蒙古羊,而蒙古羊所产羔羊的成活率显著高于小尾寒羊(P<0.05);平均每只小尾寒羊母羊可提供的断奶羔羊数(0.42只)略高于蒙古羊(0.39只),小尾寒羊作为胚胎移植受体略具有优势。综合分析表明,在内蒙古兴安盟地区小尾寒羊和蒙古羊均可以作为胚胎移植的受体来使用。 相似文献