Telomerase activity in clinically normal dogs and dogs with malignant lymphoma   总被引：3，自引：0，他引：3  

Carioto LM  Kruth SA  Betts DH  King WA 《American journal of veterinary research》2001,62(9):1442-1446

OBJECTIVES: To determine whether telomerase activity was present in lymph nodes, buffy coat, and serum samples from dogs with malignant lymphoma (ML) and in liver, lymph node, buffy coat, and serum samples from clinically normal dogs SAMPLE POPULATION: Tissue specimens and blood samples were obtained from 11 clinically normal adult dogs (age range, 1 to 4 years) and 14 client-owned dogs with ML. PROCEDURE: The telomere repeat amplification protocol assay was used to quantify telomerase activity in the tissues from clinically normal dogs and dogs with ML. RESULTS: Of 11 clinically normal dogs, 8 had lymph node samples, 5 had liver samples, and 1 had buffy coat samples with detectable telomerase activity. None of the serum samples from the clinically normal dogs had detectable telomerase activity. Of 14 dogs with ML, 9 had lymph node samples, 3 had buffy coat samples, and 1 had serum samples with measurable telomerase activity. CONCLUSIONS AND CLINICAL RELEVANCE: Telomerase activity was not specific to tumor cells and overlapped with that found in cells from clinically normal dogs. Telomerase activity in neoplastic lymph nodes was not substantially different from that found in lymph nodes from clinically normal dogs. The determination of telomerase activity cannot be used as a sole diagnostic test for cancer. Therapeutic modalities directed toward the telomerase enzyme may not be feasible in dogs, because somatic tissues from clinically normal dogs possess variable amounts of telomerase activity.  相似文献   

The need and requirements for WHO swine viral reagents     

A O Betts 《Journal of the American Veterinary Medical Association》1972,160(4):662-664

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In vitro analysis of antiangiogenic activity of fungi isolated from clinical cases of equine keratomycosis     

Welch PM  Gabal M  Betts DM  Whelan NC  Studer ME 《Veterinary ophthalmology》2000,3(2-3):145-151

OBJECTIVE: The goal of this project was to explore the possibility that fungal organisms produce metabolites that inhibit angiogenesis. Procedures Fungal cultures were obtained from cases of keratomycosis, grown in Sabouraud's dextrose broth, and sterile filtered for use in experiments. The Matrigel assay was used to screen the filtrate samples for antiangiogenic activity. Matrigel is a basement membrane matrix that supports the differentiation of human umbilical vein endothelial (HUVE) cells into a capillary-like network of tubules. HUVE cells were cultured using standard techniques and passaged at confluence, with all cells being used at passage 3-6. HUVE cells (40 000 cells) were pipetted into each well of a 24-well tissue-culture plate coated with Matrigel. An aliquot of fungal media filtrate was added to each well and the plates allowed to incubate for 18 h, at which time they were evaluated for tubule formation. RESULTS: Two fungal isolates showed inhibition of tubule formation. The addition of 100, 200 and 400 &mgr;L of the fungal media filtrate from the first isolate (Fusarium sp. 99A34574) produced a consistent and dose-dependent inhibition of tubule formation. The second isolate (Aspergillus sp. 271599) did not show inhibition of tubule formation with 100 or 200 &mgr;L added to the wells, however, it did show inhibition at 400 &mgr;L/well. The remaining three isolates did not cause inhibition at any concentration. CONCLUSIONS: Our findings suggest that certain fungal organisms produce metabolites that inhibit tubule formation in vitro, and that these metabolites may play a significant role in altering the host vascular response to fungal infections of the cornea.  相似文献   

Vaccination of dogs with multi-component vaccines     

DH DAVIES  S. PIDFORD 《Australian veterinary journal》1991,68(5):183-184

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SECTION ON BOTANICAL SCIENCES (G) AND AFFILIATED SOCIETIES     

Buchholz JT  Avery GS  Betts EM  Schopf JM  Burkholder PR  Jones CH  Barss HP  Pinckard JA  Studhalter RA  Loomis WE  Whitney EG 《Science (New York, N.Y.)》1940,91(2353):112-116

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SECTION ON BOTANICAL SCIENCES (G) AND AFFILIATED SOCIETIES     

Buchholz JT  Burkholder PR  Betts EM  Andrews HN  Fogg JM  Pinckard JA  Loomis WE  Couch JN  Voth PD  Gleason HA  Tryon RM 《Science (New York, N.Y.)》1941,93(2406):128-131

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Synthesis of Novel Thin-Film Materials by Pulsed Laser Deposition     

DH Lowndes  DB Geohegan  AA Puretzky  DP Norton  CM Rouleau 《Science (New York, N.Y.)》1996,273(5277):898-903

Pulsed laser deposition (PLD) is a conceptually and experimentally simple yet highly versatile tool for thin-film and multilayer research. Its advantages for the film growth of oxides and other chemically complex materials include stoichiometric transfer, growth from an energetic beam, reactive deposition, and inherent simplicity for the growth of multilayered structures. With the use of PLD, artificially layered materials and metastable phases have been created and their properties varied by control of the layer thicknesses. In situ monitoring techniques have provided information about the role of energetic species in the formation of ultrahard phases and in the doping of semiconductors. Cluster-assembled nanocrystalline and composite films offer opportunities to control and produce new combinations of properties with PLD.  相似文献   

Bacterial and mycoplasmal flora of the healthy camelid conjunctival sac     

J R Gionfriddo  R Rosenbusch  J M Kinyon  D M Betts  T M Smith 《American journal of veterinary research》1991,52(7):1061-1064

Healthy conjunctival sacs of 88 animals of 3 species of captive camelids (Lama glama, Lama guanicoe, Lama pacos) and llama-guanaco hybrids were sampled for bacterial and mycoplasmal flora. Mycoplasmas were not isolated from any animal. Eleven genera of bacteria were isolated. The most frequent isolates were Staphylococcus epidermidis and Pseudomonas spp. Nine varieties of Pseudomonas were found, which represented at least 3 Pseudomonas species. Many of the bacterial isolates (especially the pseudomonads) are potential pathogens in the eyes of these camelids.  相似文献   

The recognition and prevalence of Bacteroides nodosus serotype M in Australia and New Zealand     

DH CHETWIN  LC WHITEHEAD  SEJ THORLEY 《Australian veterinary journal》1991,68(4):154-155

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The effect of pregnant mare serum gonadotrophin on ovulation rate in Scottish Half-bred and Scottish Blackface ewes   总被引：1，自引：0，他引：1  

J E Newton  H L Denehy  J E Betts 《The British veterinary journal》1972,128(1):35-42

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