共查询到20条相似文献，搜索用时 31 毫秒
1.
雏鹅新型病毒性肠炎病毒强毒在感染鸭胚体内形态发生学和宿主细胞超微病理学研究 总被引：1，自引：0，他引：1
将雏鹅新型病毒性肠炎病毒（NGVEV）强毒CH株经尿囊腔途径人工感染10日龄鸭胚,应用透射电镜和超薄切片技术研究病毒在宿主细胞内的形态发生及各组织器官的超微结构变化。结果表明：感染后不同时间剖杀及死亡鸭胚的尿囊膜、肠、心、肝、脑和肌胃组织中,均观察到60～70nm的病毒粒子。病毒粒子主要通过与细胞膜融合而进入细胞质内,然后在细胞核内进行复制和装配。最后病毒粒子通过核膜和细胞膜破裂的方式被释放。病毒侵害的主要靶细胞包括鸭胚尿囊膜上皮细胞、肠上皮细胞、肠道平滑肌细胞、成纤维细胞、肝细胞、肌胃黏膜上皮细胞和心肌细胞等,表现为细胞核内外膜间隙严重扩张,细胞质整体结构严重空化。病毒侵害的主要靶细胞器包括粗面内质网和线粒体,表现为粗面内质网扩张呈囊状;尿囊膜上皮细胞的线粒体出现固缩和异常聚集变化,而其他组织细胞的线粒体均表现为肿胀和嵴断裂、消失。本试验还发现NGVEV可诱导宿主细胞发生严重的细胞凋亡现象,表现为细胞皱缩,胞核内染色质密度增高,核固缩成一个或数个团块凝聚在核膜周边,胞质浓缩深染并形成凋亡小体。 相似文献
2.
鸭病毒性肠炎病毒CH强毒株感染鸭胚成纤维细胞的超微结构观察 总被引：4，自引：0，他引：4
通过超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒（DEV）CH强毒株在鸭胚成纤维细胞（DEF）中的形态结构进行了研究。结果发现，DEVCH强毒株病毒核酸呈圆形颗粒状，直径35～45nm，在胞核内常集中分布；病毒核衣壳呈圆形．直径90～100nm．在胞核和胞浆内都有分布；DEV核衣壳可根据所含核酸形态的差异分为空心核衣壳、内壁附有颗粒型核衣壳、同心圆形核衣壳和实心核衣壳；成熟的病毒粒子具有囊膜和皮层结构．呈圆形．直径150～300nm，存在于胞浆空泡内；DEV可在DEF中分别形成胞浆内和胞核内包涵体结构；伴随子代病毒在细胞内的出现，胞浆内迁出现豆英状、马蹄形、半圆形、圆形、同心圆形等与病毒发生有关的电子致密结构。 相似文献
3.
研究通过HE染色镜检发现,鸭病毒性肠炎病毒CH强毒株(DEV-CHv)可致鸭胚成纤维细胞(DEF)出现染色质颗粒化、细胞核变形等显著凋亡特征;TUNEL试验显示,接毒组细胞核内有多量棕黄色DAB显色颗粒,表明细胞出现了凋亡现象;DNA Ladder检测表明,接毒组细胞具有分子量分别为180～200bp及其整数倍的凋亡梯带电泳图谱特征;电镜观察发现,接毒组细胞具有染色质浓缩、边移,胞浆严重空泡化,细胞核严重变形,形成凋亡小体等典型凋亡特征.上述研究结果表明鸭病毒性肠炎病毒具有显著的致鸭胚成纤维细胞发生凋亡的作用. 相似文献
4.
Shun Chen Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Qihui Luo Hengmin Cui Yi Zhou Yin Wang Zhiwen Xu Zhengli Chen Xiaoyue Chen Xiaoyu Wang 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):333-339
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease. 相似文献
5.
鸭病毒性肠炎病毒CH强毒株在鸭胚成纤维细胞中形态发生学的研究 总被引：5，自引：2，他引：3
采用超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒CH强毒株（DEV-CHv）在鸭胚成纤维细胞（DEF）中的形态发生学进行了研究。结果表明，DEV-CHv吸附到DEF细胞上以后通过囊膜和细胞膜间发生融合的方式侵入宿主细胞；病毒在细胞核内完成病毒核酸的生成、核酸与衣壳的装配；病毒核衣壳通过核膜-内质网膜系统从细胞核转运到内质网池中并在内质网池中得到皮层结构；获得了皮层的DEV-CHv核衣壳通过出芽方式释放到细胞空泡内得到囊膜结构后成为成熟病毒；细胞空泡内的成熟DEV-CHv可通过细胞的胞吐作用被释放到细胞外，或在空泡破裂时被释放到细胞外。 相似文献
6.
Yufei Guo Chanjuan Shen Anchun Cheng Mingshu Wang Na Zhang Shun Chen Yi Zhou 《Veterinary microbiology》2009,138(3-4):258-265
Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis. 相似文献
7.
Detection and Localization of Aleutian Disease Virus and its Antigens in vivo by Immunoferritin Technique 
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下载免费PDF全文 Tissues from mink infected with aleutian disease virus were examined by the electron microscope for the presence of virus particles. Virus-like particles, measuring 22 nm in diameter, were observed in macrophages of spleen, mesenteric lymph node and in Kupffer cells in liver of mink ten to 13 days after infection. The virus-like particles were usually present in vacuoles inside the cytoplasm of macrophages and Kupffer cells and, occasionally, similar particles were observed inside the nucleus. Cells from uninfected mink did not contain such patricles. To correlate the existence of these virus-like particles with the presence of aleutian disease virus antigen in infected cells, tissues were processed for immunoferritin technique. It was found that aleutian disease virus antigen was present in vacuoles inside the cytoplasm of cells from the infected spleen, lymph node and liver, and that the location was similar to that of the 22 nm virus-like particles. In addition, some viral antigen was also detected as cytoplasmic granular material. The nuclei of some cells also contained aleutian disease virus antigen. The pattern of aleutian disease virus antigen was similar to the distribution of virus-like particles in cells of infected tissue. It is suggested that virus replication occurs inside the nucleus with subsequent accumulation of virus in the vacuoles of the cytoplasm. 相似文献
8.
为研究鸭病毒性肠炎病毒(DEV)CH强毒株在感染鸭体内的分布和形态学发生规律，应用透射电镜和超薄切片技术对人工感染DEV的成年鸭各组织器官进行观察。结果表明：感染后12h在脾脏和法氏囊首先观察到少量的DEV出现，24h后在脾、胸腺和法氏囊以及死亡鸭的肝、肠和胰中均观察到具有典型的疱疹病毒粒子及其核衣壳形态的DEV。DEV病毒核衣壳有空心型、致密核心型、双环型和内壁附有颗粒型4种形态，存在胞核和胞浆两种装配方式。病毒成熟有两种方式：一为细胞核内核衣壳在核内获得皮层，通过核内膜获得囊膜成为成熟病毒；二为核内核衣壳通过内外核膜进入胞浆，核内和胞浆内的核衣壳在细胞浆中获得皮层，然后在各种质膜上获得囊膜，最后成熟病毒通过细胞破裂或其他方式释放到细胞外。伴随着病毒的复制、装配和成熟，细胞中出现多种核内和胞浆包涵体、核内致密颗粒、核内微管和中空短管、胞浆电子致密小体等结构。 相似文献
9.
In situ hybridization was applied to detect fowlpox virus (FPV) DNA in formalin-fixed paraffin-embedded sections of the skin from infected chickens by using a biotinylated probe and a streptavidin-alkalinephosphatase conjugate. The immunohistochemical examination was applied to compare the distribution of the FPV DNA to that of related antigenic protein in serial sections. In the infected epithelial cells, FPV DNA was detected in cytoplasmic inclusion bodies and in the rest of cytoplasm. Likewise, immunohistochemical examination revealed the virus antigen in cytoplasm. Ultrastructurally, virions were observed in the cytoplasmic inclusion bodies, and immature virus particles were in the rest of the cytoplasm. The study proved restricted distribution of FPV DNA in the cytoplasm. 相似文献
10.
对猪细小病毒(PPV)、牛疱疹病毒2型(BHV2)和犬腺病毒1型(CAV1)3种动物DNA病毒在宿主细胞内的增殖、释放方式以及所致细胞结构的变化,通过电镜进行了观察比较.(1)这3种动物DNA病毒的复制和装配过程均发生在细胞核内,以毒浆结构(Viroplast)或核内包涵体为增殖场所和物质基础,但并非都形成结晶样结构.(2)有囊膜的BHV2,其核壳体在细胞核内装配完成后,从核内膜上以出芽方式获得囊膜,然后进入核周池,聚集的病毒使核外膜向胞质方向隆起,形成病毒性包涵体而脱离核外膜,并逐渐向细胞膜的方向移动,最后从细胞膜的破损处以病毒包涵体形式释放到细胞间隙.而无囊膜的CAV1,核壳体在细胞核内装配完成后,从细胞核膜破损处或细胞核崩解后进入细胞质,待整个细胞崩解后才能释放出来.无囊膜的PPV,在核壳体装配完成后,成堆地以病毒流的方式,从扩张的核孔释放到细胞质中,待细胞崩解后再释放出来.(3)3种病毒增殖时,宿主细胞的固有细胞器,如线粒体、内质网以及溶酶体等均出现不同程度的超微结构变化,并能诱导宿主细胞出现一些新的结构,除毒浆结构外,还有管状结构、细纤维样结构、周期性结构和髓膜样结构等,其中周期性结构仅见于BHV2感染. 相似文献
11.
人工感染IBDV鸡法氏囊的电镜研究 总被引：7，自引：0，他引：7
通过透射电镜系统观察了人工感染传染性法氏囊病病毒（ＩＢＤＶ）后鸡法氏囊各类细胞的病理变化。感染后１２￣２４ｈ,病毒粒子主要见于髓质淋巴细胞中,细胞中可见到大量纤维样病毒发生基质及无囊膜包围的大型病毒晶格,细胞核染色质浓缩,核中出现纤维样结构。感染后３６ｈ,淋巴细胞开始大量裂解死亡。无囊膜包围的病毒晶格也出现于髓质网状细胞中,被感染的网状细胞并不裂解,而表现出细胞凋亡的特征：染色质固缩呈颗粒块状,胞 相似文献
12.
The morphogenesis of duck enteritis virus (DEV) and distribution in vivo were observed by electron microscopy after ducks were infected experimentally with DEV virulent strain. The investigation showed that a few typical herpesvirus virions and nucleocapsids were first observed in the spleen, thymus, and bursa of Fabricius (BF), and many nucleocapsids, mature viruses, and viral inclusion bodies could be found in the nucleus and cytoplasm of infected liver, small intestine, spleen, thymus, and BF when the ducks died. Nucleocapsids assembled both in nucleus and cytoplasm and could be divided into four different types according to their structures. Typical herpesvirus, light particles (L-particles), and virions without tegument could be observed at the same time. With the replication, assembly, and maturation of the viruses, intracytoplasmic and intranuclear inclusion bodies, electron-density particles, microtubules, hollow tubes, and coated electron-density bodies were observed in infected cells. 相似文献
13.
兔出血症病毒在细胞培养和组织中的形态发生 总被引：4，自引：2，他引：2
在电镜下系统地观察了感染后的细胞培养和组织中兔出血症病毒（ Ｒ Ｈ Ｄ Ｖ）的形态发生。感染早期，在细胞核内可见电子致密颗粒（１５ ｎｍ ）和未成熟的病毒颗粒 （２５ ｎｍ ）。中期，在细胞核和胞浆内出现大量成熟的病毒颗粒（３４ ｎｍ ），并发现部分核内病毒通过扩大的核膜孔、核膜溶解扩大的核孔和乳头状突起的核膜向胞浆释放。感染末期，核染色质消失，核内大量感染病毒清淅可见。最终细胞溶解，病毒颗粒释放至细胞间隙。提示 Ｒ Ｈ Ｄ Ｖ 是在核内复制和装配的，应归属于细小病毒科。本试验结果不排除同时存在另一种小 Ｒ Ｎ Ａ 病毒。 相似文献
14.
伪狂犬病病毒吉林分离株感染BHK-21细胞的超微结构变化 总被引：1，自引：0，他引：1
以猪伪狂犬病病毒(PRV)吉林分离株PRV-JL感染体外培养的BHK-21细胞为模型,通过透射电镜对PRV的形态发生学和宿主细胞超微结构的动态变化规律进行研究。结果显示,PRV能导致BHK-21细胞圆缩,并发生细胞融合,形成合胞体;电镜观察到的病毒粒子呈球形或椭圆形,成熟的病毒粒子直径大小为140~210 nm,未成熟病毒粒子直径为90~150 nm,多呈中空状,部分呈致密核芯。病毒吸附于细胞后以膜融合的方式进入细胞,在胞核内复制,装配好的病毒粒子以出芽的方式离开细胞核,获得最初的囊膜,进入胞浆;在胞浆内的病毒粒子又利用高尔基体的膜结构合成第2层囊膜,形成完整的病毒粒子;最后包裹有完整病毒粒子的高尔基囊泡与细胞膜发生融合,将病毒粒子释放到细胞外。感染细胞超微结构变化主要表现为:细胞胞浆空泡增多,内质网扩张,线粒体增生、嵴肿胀、脱落,最后空泡化,整个细胞裂解、破碎。 相似文献
15.
传染性囊病病毒诱导细胞凋亡的初步观察 总被引：5，自引：0，他引：5
用１株ＩＢＤＶ强毒株感染易感小鸡，对病鸡法氏囊进行电镜观察及ＤＮＡ电泳分析，直接观察到病鸡法氏囊中Ｂ淋巴细胞凋亡的典型形态学特征和生化变化：染色质凝聚成团，集于核膜旁，胞膜与核膜出现凹陷，细胞拉长变形，最后细胞裂解成由膜包围着的小团，被网状细胞和巨噬细胞吞噬；感染ＩＢＤＶ２４～４８ｈ的法氏囊细胞总ＤＮＡ在电泳谱上呈梯状条带，而从正常的法氏囊提取的总ＤＮＡ在电泳谱上只有１条带。结果表明，ＩＢＤＶ感染小鸡之后，导致了法氏囊中Ｂ淋巴细胞的凋亡。作者据此推断，细胞凋亡是造成Ｂ淋巴细胞数量减少，从而导致小鸡免疫抑制的原因 相似文献
16.
Blanco A Moyano R Vivo J Flores-Acuña R Molina A Blanco C Monterde JG 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(6):286-287
Purkinje cerebellar cells were studied in three Arabian horses aged between 6 and 8 months with clinical disorders in their movements, tremors and ataxia; the occurrence of apoptosis in this cell population was investigated by the (terminal deoxynucleotidyl transferase biotin-dUTP nick-end labelling (TUNEL) method. Both optical and electron microscopical images showed a scant number of Purkinje cells, most of them with morphological features of apoptosis such as condensation of the nucleus and cytoplasm as well as segregation and fragmentation of the nucleus into apoptotic bodies. The TUNEL technique revealed a substantial number (65%) of positive immunoreactive Purkinje cells. 相似文献
17.
Homogenized lung tissue was used to experimentally reproduce lethal viral pneumonia in guinea pigs. The resultant lesions corresponded with those of the spontaneous disease. Pneumonia with necrotic bronchiolar epithelium accompanied by basophilic intranuclear inclusion bodies was the primary finding. With transmission electron microscopy, numerous viral particles were found--mainly within the nucleus of pulmonary epithelial cells but also within the cytoplasm and in extracellular space. The appearance of viral particles, in particular their paracrystalline and crystalline deposition within the nucleus, indicates that our experimentally induced pneumonia was caused by an adenovirus. 相似文献
18.
采用HE、Giemsa染色、透射电镜以及DNA琼脂糖凝胶电泳等研究了水泡性口炎病毒（VSV）诱导BHK-21细胞凋亡的过程。结果显示：VSV感染BHK-21细胞后,光镜下可见细胞圆缩,细胞器固缩、核仁消失、染色质凝聚和核碎裂、凋亡小体出现;电镜下观察到染色质聚集形成典型的新月形,胞浆中充满大量空泡,细胞核因染色质凝聚也发生了空泡化;1％的琼脂糖凝胶电泳出现180-200bp整倍数的DNA梯形条带。结果表明,VSV诱导BHK-21细胞凋亡是其致细胞病变的主要表现形式之一。 相似文献
19.
应用透射电镜和超薄切片技术观察鸭病毒性肠炎病毒（Duck Enteritis Virus，DEV）CH强毒株人工感染成年鸭各组织器官的形态学特征。结果表明，脾脏、胸腺、法氏囊、十二指肠固有层中淋巴细胞除了坏死变化外还有很明显的凋亡变化，两者往往同时存在。疾病过程中，淋巴细胞凋亡数量明显增多。凋亡细胞的形态学改变是细胞体积缩小，细胞器结构正常，染色质初期浓集成团，聚集于核膜的周边，随后出现染色质凝聚，核碎裂以及凋亡小体形成等现象。淋巴细胞的坏死和凋亡共同造成了淋巴细胞的大量损耗，淋巴细胞的这种变化可能在鸭病毒性肠炎的发病机制中起着重要的作用。研究结果表明DEV急性感染可诱导成年鸭体内淋巴细胞的凋亡。 相似文献
20.
The aim of the study was to verify whether the in vitro senescence process of tissue pool neutrophils of the bovine mammary gland is accompanied by similar changes of ultrastructure as typically occurs in in vivo conditions. The experiments were carried out in four clinically healthy, Holstein x Bohemian Red-Pied crossbred heifers aged 14-16 months. With the aid of transmission electron microscopy (TEM), scanning electron microscopy (SEM) and flow cytometry (FCM), neutrophil apoptosis in vivo was detected and during senescence it was monitored in vitro. The neutrophil apoptosis comprised three ultrastructurally different stages: (1) karyopyknosis, (2) zeiosis, and (3) apoptotic bodies. These stages were obvious in the apoptotic neutrophils both in vivo and in vitro. In addition to the common morphological signs, however, ultrastructural differences were also detected in apoptotic neutrophils in vitro. These in vitro ultrastructural differences mostly comprised hyper-vacuolation of the cytoplasm with mega-vacuoles and secondary necrosis of apoptotic neutrophils. Morphological features of apoptosis during in vitro senescence of tissue pool neutrophils of the bovine mammary gland were shown to be in close accordance with these in vivo signs. 相似文献