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1.
通过电解质外渗法和匍匐茎恢复试验对‘阳江’狗牙根及其12个通过形态鉴定选出的坪用价值高且花序密度低的诱变后代进行抗寒性鉴定。电解质外渗法结果表明:诱变后代间的抗寒性具有较大差异,其叶片半致死温度(LT50)的变异范围为-7.6~-0.2℃(最低值与最大值相差7.4℃);参试材料抗寒性由强到弱依次为M18>M4>M26>M28> M22>阳江>M29>M31>M10>M37>M16>M1>M25,其中,有5个诱变后代抗寒性优于亲本,分别是M18、M4、M26、M28、M22。匍匐茎恢复实验结果表明:诱变后代M1、M22、M26、M31、M25在0℃和-5℃低温胁迫后的恢复生长率都高于亲本,恢复能力均优于亲本;M10、M37、M28在-5℃低温胁迫下,恢复生长能力低于亲本,抗寒性相对较弱,M16和M4在0℃和-5℃低温胁迫下,恢复生长率都低于亲本,抗寒性明显弱于亲本。综合2种方法鉴定结果显示:诱变后代M1、M25的恢复能力较强;M4、M28的叶片抗寒性较好,青绿期较长;M22、M18、M26的叶片抗寒性和匍匐茎恢复能力均较强;M29,M31的叶片抗寒性和匍匐茎恢复能力与亲本相似;M10、M16、M37的叶片抗寒性和匍匐茎恢复能力均较弱,整体抗寒性较弱。  相似文献   

2.
Irrigation of Netted Gem potatoes was scheduled during three growing seasons by three methods: (a) when plants displayed first visual symptoms of moisture stress, (b) when indicated by a soil moisture budget involving estimated evapotranspiration, and (c) on the basis of tensiometer readings of soil moisture suction. When the tensiometer method of scheduling was used, the mean yields of tubers were 55.0 and 25.8 cwt/acre (6160 and 2890 kg/ha) higher than those obtained with the other two scheduling methods. Methods did not affect the specific gravity of potatoes. Method (a) scheduled irrigations least frequently. Scheduling by the budget method was not always adequate because it was based on the assumption that the crop extracted water from a constant 4 ft (1.2 m) profile from planting to full vegetative growth. In one year the budget method scheduled the first irrigation earlier than necessary and delayed the second irrigation during a critical period of crop growth. From full vegetative cover to harvest the irrigation schedules were alike for both the budget and tensiometer methods.  相似文献   

3.
In the western Sahel, indigenous plants become important staples when cereal harvests are inadequate to support populations inhabiting that region of Africa. The purpose of this study was to assess the nutrient content of several of these edible wild plants. The leaves of the following seven plant foods were analyzed: Ziziphus mauritiana, Cerathotheca sesamoides, Moringa oleifera, Leptadenia hastata, Hibiscus sabdarifa, Amaranthus viridi, and Adansonia digitata. The fatty acid, vitamin E, carotenoid, selected mineral and amino acid contents of these plant foods were determined. These same analyses were performed on the fruit of the Adansonia digitata. In quantitative and qualitative terms, Amaranthus viridis was found to be an excellent source of protein. Its amino acid composition compared favorably to that of a World Health Organization (WHO) protein standard. It also contained considerable amounts of the two fatty acids that are essential in humans (linoleic and -linolenic) and a number of minerals including iron, magnesium, calcium and zinc. The leaves of Hibiscus sabdarifa contained an appreciable quantity of protein the composition of which was comparable to the WHO standard. The mineral content of the leaves of this plant was also exceptionally high; noteworthy was its high zinc content. H. sabdarifa also contained significant quantities of the two essential fatty acids. Ziziphus mauritiana was an excellent source of the essential fatty acid linoleic acid and several of the metals including iron, calcium, magnesium and zinc. Its content of other essential nutrients, however, was rather low. In general, Adansonia digitata leaves were nutritionally superior to the fruit of the tree; however, the fruit did contain useful quantities of potassium, phosphorus, zinc and -linolenic acid. The Leptadenia hastata leaves were an especially good source of lutein and -carotene. These data should be useful to the people who inhabit the western Sahel in helping them devise healthy diets during times when cereal staples are in short supply.  相似文献   

4.
橡胶树丛枝病病原的抗血清制备与应用   总被引:5,自引:1,他引:4  
以感染橡胶树丛枝病病原的长春花为材料,制备得到橡胶丛枝病病原菌抽提液,以抽提液为抗原免疫家兔,制备抗血清,经微量沉淀测定,抗血清效价为1:2048。应用橡胶丛枝病抗血清检测橡胶褐皮病,无症苗木检出率达30%-37%,可疑的褐皮病树检出率达85.7%。  相似文献   

5.
MADS-box基因家族广泛分布于真核生物中,巴西橡胶树的MADS-box基因家族主要参与花形态建成,对生殖生长起到重要的调节作用。目前,MADS-box基因家族的26个相关基因已被克隆分析,但它们在染色体上的具体位置还未确定。本研究以巴西橡胶树‘热研7-33-97’品种为材料,将MADS-box基因家族的6个成员(HbAGL8HbAG15HbAGL30HbTT16HbAP1HbSVP1)定位在细胞核染色体上,通过双探针荧光原位杂交技术(FISH)对巴西橡胶树MADS-box基因家族的这6个成员在细胞核染色体上进行物理定位分析。结果表明:MADS-box基因家族的6个基因分别位于不同的染色体上,其中HbAGL15HbAG8HbAG30HbSVP1基因定位在第4、5、7和8号染色体长臂上,其信号位点到着丝粒的平均百分距离是11.85、39.71、48.94和6.70;HbTT16HbAP1基因定位在第1和13号染色体短臂上,其信号位点到着丝粒的平均百分距离是22.19和18.01。本研究结果揭示了巴西橡胶树MADS-box基因家族的6个成员在细胞核染色体上的实际位置,展现家族基因之间的分布特点和连锁遗传关系,不仅丰富了橡胶树分子细胞遗传学信息,也为橡胶树的分子辅助育种和比较基因组学研究提供了分子细胞遗传学的科学理论依据。  相似文献   

6.
2002~2003年在0~3次中耕条件下研究了中耕方式对玉米生长发育和产量形成的影响。结果表明,多次中耕增大了玉米倒伏率,降低了玉米最大叶面积指数、株高及各时期叶片叶绿素含量,对花粒期玉米净光合速率、气孔导度、蒸腾速率也产生一定的负面影响。少中耕有增产的趋势,并且可以降低生产成本、增加经济效益。  相似文献   

7.
东北三省玉米气候适宜度变化分析   总被引:6,自引:1,他引:5  
利用东北三省25个农业气象观测站平均资料及176个气象站1961~2007年的逐日气象资料,对玉米全生育期及各主要玉米生育阶段的气候因子及综合气候适宜度进行计算分析。结果表明,自1961年以来,玉米各发育期对气候因子适宜程度不同,播种期-出苗期、出苗期-开花期、开花期-成熟期温度适宜度呈上升趋势,日照适宜度呈下降趋势;播种期-出苗期降水呈上升趋势,其余各生育期降水均为下降趋势。玉米全生育期降水、日照适宜度呈下降趋势,温度适宜度呈上升趋势,综合气候适宜度东北三省均呈下降趋势。各气候因子组合效果较好,玉米产量与各生育阶段的不同气候因子的适宜度相关性差异较大。  相似文献   

8.
对造成福建省漳州市东山县的芦笋枯萎病病原菌进行分子鉴定,以期确定病原菌的属、种名,为防治该病害寻求理论依据。试验对分离纯化的病原菌核糖体DNA的ITS区进行测序,在Genbank中搜索其同源性并构建它们的系统发育树,结果表明:引起芦笋枯萎病的病原菌是镰刀属中的尖孢镰刀菌(Fusarium oxysporum),且病原菌之间的同源性高达89%,遗传差异不显著。  相似文献   

9.
A broadcast application of PCNB (Terraclor) resulted in a reasonably uniform distribution of the chemical in the soil with the concentration decreasing in a linear fashion with increasing depth in the soil profile. A banded application resuluted in a considerably higher chemical concentration at the 4–6 inch depth than at the 0–2 or the 2–4 inch depths. The problem of the lack of uniformity of chemical distribution could probably be solved by altering nozzle placement and size and allow use of the band method of application to minimize grower cost. More complete disking in the case of the broadcast treatment would probably result in a relatively uniform distribution of the chemical but at higher cost to the grower. PCNB application rates of 10, 15 and 25 lbs per acre broadcast and 71/2, 10 and 121/2 lbs per acre in a band significantly reduced the severity ofRhizoctonia infection of Russet Burbank potatoes but did not increase potato yields significantly.  相似文献   

10.
麦芽糖提高水稻花药培养效率的研究   总被引:33,自引:2,他引:33  
 研究了浓度均为150 mmol/L的9种碳源(含组合)对3个籼稻品种花药的愈伤组织诱导率的影响,发现麦芽糖为碳源的培养基效果最佳。进一步以7个籼稻2个粳稻和4个杂交组合为材料,系统比较了150 mmol/L的麦芽糖与蔗糖作为诱导培养基的碳源对花药培养的影响,结果表明,以麦芽糖为碳源的培养基上,平均愈伤组织诱导率和随后的绿苗分化率分别为54.81%与9.70%,而在相应的蔗糖培养基上只有11.85%和3.30%,差异极显著。讨论了提高籼稻花培效率的簧略,井建议采用统一的方法计算愈伤组织诱导率.  相似文献   

11.
This study was designed to follow patients regarding to achieving control, identify disease exacerbations and discover relation between exacerbation and risk factors. In a cross sectional-analytic study, 400 asthmatic patients in the range of 2-14 years old who were visited at the specialized outpatient clinic of Tabriz University of Medical Sciences were divided and studied on the base of disease severity. At the end (at least one year treatment), patients were categorized based on level of disease control and one year after this stage, cases of exacerbation were identified and studied. By excluding patients with poor adherence, 342 patients continued treatment as intermittent, mild persistent, moderate persistent and severe persistent asthma (38, 235, 61 and 8 patients, respectively). After a period of one year treatment, the patients were divided as well controlled, partly controlled and uncontrolled (68, 30 and 2%, respectively). Cases of disease exacerbations were more if sinusitis, familial asthma history and poor adherence were present (p < 0.01, p < 0.001 and p < 0.05, respectively). There is meaningful association between poor adherence, sinusitis and positive family history of asthma with disease exacerbation.  相似文献   

12.
波罗蜜叶片突变体叶绿素含量测定和超微结构观察   总被引:1,自引:0,他引:1  
波罗蜜(Artocarpus heterophyllus)是一种热带果树,至今对其突变体的研究少有报道。本研究以波罗蜜叶片为试验材料,探究其叶片出现白化和返绿现象的可能原因。(1)用分光光度计法、比色法测定波罗蜜叶片突变体和正常绿叶的叶绿素及叶绿素前体物质含量;(2)用叶绿素酶Elisa试剂盒测定叶绿素酶活性;(3)用透射电子显微镜对叶绿体的超微形态结构进行观察。研究结果表明:(1)不同叶色所产生的叶绿素a含量、叶绿素b含量、叶绿素总含量、类胡萝卜素含量均存在显著差异;(2)波罗蜜叶片突变体和正常绿叶的叶绿素合成前体物质含量之间并没有出现显著性差异;(3)对波罗蜜叶片的叶绿素酶活性进行测定时,发现其活性出现显著差异,但对其叶绿素含量的差异性并没有产生较大影响;(4)观察波罗蜜叶片内叶绿体超微形态结构时,发现正常绿叶的叶绿体形态完好且数量较多,白化叶和返绿叶的叶绿体内部结构存在缺陷,其原因是叶绿体基粒构建阶段受阻;基于测定波罗蜜叶片中的叶绿素、叶绿素前体物质含量和叶绿素酶活性,并观察波罗蜜叶片内叶绿体的超微形态结构,对得到的数据结果进行比较分析。本研究推测是在叶绿素合成阶段,原脱植基叶绿素合成叶绿素时受阻及叶绿体发不良导致波罗蜜出现白化和返绿现象,为今后进一步综合研究波罗蜜突变体提供理论依据。  相似文献   

13.
Data from in vivo digestibility trial with four to six horses fed twenty-seven forage-based diets are used to calculate prediction equations for the digestibility of dry and organic matter, based on the crude ash (CA), crude protein (CP) and crude fibre (CF) contents of diets and faeces. The most precise prediction of dry-matter digestibility (r.s.d. = 0.032, R2= 0.80) was derived from a multiple regression including faecal (CP, CF) and dietary parameters (CF). Among faecal parameters, CP was the best single predictor of both digestibility (r.s.d) = 0.040, r2= 0.63) and dietary CP content (r.s.d = 0.028, r2= 0.59). For biological reasons we propose a non-linear model that allows prediction of dry- and organic-matter digestibility from faecal CP Content with reasonable Precision (r.s.d = 0.038, 0.036, r2= 0.65, 0.74, respectively). This will be adequate for many studies, especially for free-living animals in rangelands.  相似文献   

14.
粤西地区橡胶树炭疽病流行因素分析   总被引:2,自引:0,他引:2  
对橡胶树炭疽病发生和流行的调查、观察结果表明,病菌越冬场所广泛,其寄主带菌率高(40%-100%)。一年中只要抽嫩叶病菌都可以侵染为害,在橡胶树抽第一蓬叶时遇上雨天高湿环境病害就可注行。分析粤西地区20a资料发现,嫩叶期间的雨日和>90%RH的天数、嫩叶期长短、嫩叶期寒潮(11-15℃)的天数及最低温度在11℃以下的天数与寅害流行密切相关。嫩叶期间的雨日和>90%RH的天是病害流行的主导因素,低温阴雨天气是加速病害发展的重要条件。  相似文献   

15.
为深入研究簕杜鹃种质资源的遗传多样性和后期簕杜鹃种质资源的创制与利用提供参考,本研究对国内外收集的100份簕杜鹃种质资源的9个数量性状和19个质量性状进行测定与评价,利用相关性分析、主成分分析和聚类分析等方法,探讨其种质资源表型性状的遗传多样性。结果表明,在测定的19个质量性状中共检测到78个变异类型,28个表型性状的遗传多样性指数在0.61~2.06之间,9个数量性状的变异系数为12.38%~37.68%,以花序梗长度的变异系数最大(37.68%)。相关性分析结果表明性状之间关系复杂,大部分性状之间呈现显著相关性,其中叶片大小与苞片大小呈极显著正相关。通过主成分分析将簕杜鹃28个表型性状转化成9个主成分因子,累计贡献率达到70.42%,其中叶片长度、叶片宽度、苞片长度、苞片宽度、花冠直径、花被管长度、叶节间长度、花被管形状、株型等是造成簕杜鹃种质资源表型性状差异的主要因素。聚类分析可将100份簕杜鹃种质资源分为6类,其中第一类群包含7份簕杜鹃种质资源,该类群品种多为重瓣品种,具有较高的观赏价值,但后期苞片宿存,影响观赏性。第二类包含16份簕杜鹃种质资源,叶片大小中等,形状多为椭圆形,苞片形状以卵形为主。第三类包含10份簕杜鹃品种,该类群簕杜鹃品种苞片先端性状为渐尖,基部形状为心形,叶面多稍微上捧。第四类包含18份材料,该类群品种叶片中等,叶柄较短,苞片形态为阔椭圆形。第五类群包含8份簕杜鹃品种,该类群簕杜鹃品种苞片形状特异,叶柄短小,叶节间短缩,其中“塔类”系列簕杜鹃品种,整体形似宝塔,观赏价值较高。第六类包含41份材料,其中在欧式距离为17时,可将第六类群分为4个亚群,第1亚群仅包含1个品种,第2亚群簕杜鹃品种叶片多含有次色,第3亚群叶片较大,第4亚群叶片主要以卵形为主,叶面光滑无毛,苞片主要以卵形为主,花被管性状以纤细和中部收缩为主。本研究明确了不同簕杜鹃种质资源的农艺性状的特异性和遗传多样性,筛选具有特异性状的簕杜鹃种质资源,为簕杜鹃新品种的选育提供理论基础。  相似文献   

16.
为了探讨龙眼(Dimocarpus longan Lour.)种质果实性状的稳定性,连续3a对40份不同基因型龙眼种质果实数量性状的年际间表现进行测定比较。结果表明,在气候条件差异较大的不同年份,单果重、果皮重、果肉重、种子重、果皮厚、可食率、可溶性固形含量等果实性状均表现丰富的多样性,并存在年际间的显著或极显著水平差异,气候条件对果实数量性状有显著影响;不同性状的稳定性有差异,其中可食率的稳定性最高,其次是可溶性固形物含量。通过聚类分析,可将40份龙眼种质按果实性状稳定性分成7类。  相似文献   

17.
The main seed proteins of Pisum and faba beans are the storage proteins synthesised in the seed and subsequently utilised to supply nitrogen and carbon for biosynthesis during germination and seedling development. There are two major families of storage proteins, legumin and vicilin. Legumins are hexameric proteins comprising of 6M r 60 000 subunits which, as isolated from mature seeds, show considerable charge and size heterogeneity. Vicilins are a less well-defined group of proteins consisting largely ofM r 50 000 subunits and non-equimolar amounts of lower molecular weight subunits. A third storage protein, convicilin, is also present in peas and beans. It is a vicilin-type protein with vicilin immunological determinants. Proteins homologous to legumin have been found in Arachis, Glycine,Vigna unguiculata, Lupinus, Cicer, Lens and Lathyrus. Equivalent vicilin-type proteins occur inPhaseolus vulgaris, Glycine, Arachis andVigna unguiculata. The storage proteins have an unusual amino acid composition related to their biological role. They are multimeric and readily undergo association/dissociation reactions. They show a high degree of charge and size heterogeneity, partly caused by co and post-translational modifications and partly due to gene divergence.  相似文献   

18.
新粳型不育系和恢复系是通过籼粳交或粳粳交的方式培育而成的中间型稳定品系,杂交组合的育性正常或接近正常。粳型不育系和恢复系配组,杂种F1的产量性状差异显著或极显著。组合9512A/R364、73A/R55、150A/R198、9512A/R2690、150A/R55,具有产量的正向超亲、超标优势,另一些组合呈显著或极显著的负向优势;本研究产量性状一般配合力好的不育系是中14A、辽150A、73A、9512A、9214A,恢复系是R198-2、R238、R46、C418、R364和花38;亲本的产量性状一般配合力和特殊配合力在不同性状的作用没有交叉。杂种优势的表现与双亲亲和力高低、配合力高低有明显的相关性。辽150A、9512A的超亲优势明显,R198、R55、R2690、R364、C204等恢复系具有较强的杂交优势。不育系对产量性状的作用显著大于恢复系。  相似文献   

19.
玉米大斑病菌对广州地区甜玉米的致病性观测   总被引:2,自引:1,他引:2       下载免费PDF全文
通过对桂甜和华珍两个甜玉米品种的玉米大斑病病斑扩展速率及病情指数的研究结果表明,不同品种对玉米大斑病菌的抗性存在明显的差异;病斑的产孢特性显示,病斑首次产孢量显著高于再次产孢量。通过比较不同温度下的菌丝生长量,分析出广州地区玉米大斑病菌的主要构成属于高温型。  相似文献   

20.
Mechanism of action of insecticidal secondary metabolites of plant origin   总被引:1,自引:0,他引:1  
Insect pest management is facing the economic and ecological challenge worldwide due to the human and environmental hazards caused by majority of the synthetic pesticide chemicals. Identification of novel effective insecticidal compounds is essential to combat increasing resistance rates. Botanicals containing active insecticidal phytochemicals appear to be promising to address some of these problems. Therefore, there is a continuous need to explore new active molecules with different mechanisms of action. Secondary metabolites present in plants apparently function as defense (toxic), which inhibits reproduction and other processes. The phytochemical biomolecules could be used for maximizing the effectiveness and specificity in future insecticide design with specific or multiple target sites, while ensuring the economic and ecological sustainability. In this article, the current state of knowledge on phytochemical sources and insecticidal activity, their mechanism of action in insects, resistance, and promising advances made in phytochemical research are reviewed.  相似文献   

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