共查询到20条相似文献，搜索用时 109 毫秒
1.
【目的】利用微胶囊技术以果胶、壳聚糖为壁材对姜黄素进行包被处理,制备双层包被微胶囊,通过形态特征、机械强度、包埋率等分析确定最佳制备条件,并对其进行体外人工胃肠道耐受性研究。【方法】以8%果胶、不同浓度姜黄素(3.5、4.0、4.5、5.0和5.5 mg/mL)、氯化钙(CaCl2)溶液(0.3、0.4、0.5和0.6 mol/L)及不同配比姜黄素与果胶溶液(3∶7、2∶8、1∶9(V/V))为第一层包被,筛选果胶姜黄素微胶囊的最佳制备条件;以不同pH(4.2、4.6、5.0、5.4和5.8)的0.8%壳聚糖为第二层包被,筛选制备果胶/壳聚糖姜黄素微胶囊的最佳条件。以筛选的最佳条件制备3组微胶囊(T1、T2和T3),并对其外貌形态、机械强度、包埋率、载药量及体外人工胃肠道耐受性进行评价。【结果】当以8%果胶、4.5 mg/mL姜黄素、姜黄素与果胶配比为1∶9(V/V)、0.5 mol/L CaCl2为第一层包被条件时制得的果胶姜黄素微胶囊包埋率达98%;以pH为4.6、5.0和5.4的0.8%壳聚糖为第二层包被条件时制备的微胶囊大小均匀、形状... 相似文献
2.
《饲料工业》2016,(10)
为探究三种投喂方式对小池塘鲤草鲫混养效果的影响,以肠道健康膨化饲料(蛋白水平30%、脂肪水平4%)为试验饲料,采用三种投喂方式:自动投喂(AF)、人工投喂(MF)和自助摄食(SF),在室外小池塘(10 m×10 m×1 m)条件下,研究不同投喂方式对鲤草鲫增重率、特定生长率、存活率、摄食率、蛋白质效率和饲料系数的影响。每种投喂方式设置3个重复,每个小池塘放养300尾鲤鱼、100尾草鱼、200尾鲫鱼,鲤鱼初始体重为(93.03±0.20)g、草鱼初始体重为(176.61±6.79)g、鲫鱼初始体重为(75.83±0.08)g,每天按照相同的投喂量(体重的2%~3%)投喂4次,试验周期为8周。试验结果显示:在鲤草鲫混养条件下,自动投喂组、人工投喂组和自助摄食组的鱼类存活率、蛋白质效率和饲料系数无显著差异(P0.05)。自助摄食组的鲤鱼和鲫鱼增重率、特定生长率明显高于人工投喂组和自动投喂组(P0.05),而草鱼的情况刚好相反(P0.05)。池塘鲤草鲫混养条件下,鲤鱼由93 g生长到318 g,草鱼由177 g生长到526 g,鲫鱼由76 g生长到155 g,肠道健康膨化饲料的饲料系数平均为1.37。试验表明:池塘中鲤草鲫混养时,三种投喂方式不影响鱼类的存活率、蛋白质效率和饲料系数,自助摄食方式利于鲤鱼和鲫鱼的摄食。根据本试验,建议在混养池塘中采用不需要电力又省力的自助摄食方式。 相似文献
3.
本文以酵母粉蛋白胨培养基为基础培养基,探讨了不同培养时间、接种量、初始pH、碳源、氮源、金属离子对枯草芽孢杆菌BGJ222表达β-半乳糖苷酶能力的影响。结果表明,菌株BGJ222最大产酶量的培养条件为:初始pH 6.6,接种量2%(V/V),培养温度37℃,培养时间24 h;培养基组成为:酵母粉0.5%(m/V),蛋白胨1.0%(m/V),NaCl 0.5%(m/V),三梨醇1.0%(m/V),MgCl20.5%(m/V)。在此条件下,菌株BGJ222表达β-半乳糖苷酶达到11456.4 Miller,比基础培养基的表达量(860 Miller)提高了12倍。 相似文献
4.
4种不同粒径(3.0、4.0、5.0和6.5mm)和营养成分相同的饲料按照等颗粒数目混合,投喂3种不同规格的星斑川鲽幼鱼,体质量分别为(55±5)、(95±5)和(135±5)g,以饱食为准,研究星斑川鲽规格与摄食饲料粒径间的关系。结果表明:1)幼鱼摄食粒径6.5mm饲料出现明显的回吐饲料与吞咽困难现象,这种现象在(55±5)g幼鱼中尤为显著。2)(55±5)g幼鱼摄食率(5.73)最高,(95±5)g幼鱼摄食率(5.20)次之,(135±5)g幼鱼摄食率(4.95)最低;(95±5)g幼鱼明显加大对粒径4.0mm饲料的摄食数目,约为(55±5)g幼鱼的3倍。3)幼鱼体质量与摄食的相应饲料数目关系为Y5.0=4.6273e0.0105X(R2=0.9215),Y4.0=6.2075e0.0165X(R2=0.8445),Y3.0=24.71e0.0048X(R2=0.4827,相关性极差),X为幼鱼体质量/g,Y3.0、Y4.0和Y5.0分别为幼鱼摄食3.0、4.0和5.0mm粒径的饲料数目。综上所述,对50～140g的星斑川鲽幼鱼投喂粒径为4.0与5.0mm的饲料,其颗粒数目比Y4.0/Y5.0=(6.2075e~(0.016.5x))/(4.6273e~(0.0105x))。在星斑川鲽幼鱼的人工养殖中,可根据投喂量和单粒饲料的质量,换算为2种颗粒饲料的质量比更加便捷。 相似文献
5.
为实现功能化氧化石墨烯(graphene oxide,GO)和多种羧基化碳纳米管(carboxylated carbon nanotube,CNTs)复合物的制备及载药性能研究,首先将壳聚糖(chitosan,CS)共价修饰于各碳纳米材料表面,进一步缩合用于示踪的异硫氰酸荧光素(fluorescein isothiocyanate,FITC)及负载羟基喜树碱(hydroxycamptothecin,HCPT),随后采用Zeta电位和透射电镜进行碳纳米载药复合物的表征,紫外可见吸收光谱(UV-vis)进行载药性能及药物体外释放的研究,CCK-8法测定其对细胞的毒性。结果显示:相比多种CNTs纳米复合物,GO纳米复合物有较高的载药量(LC)和载药效率(LE),LC和LE分为1.24 mg/mg和92.7%;药物释放量与pH值相关,酸性条件(pH=5.8)下,36 h时HCPT累计释放量达到56.15%,中碱性条件(pH=7.4和pH=8.0)下,HCPT累计释放量分别是12.58%和7.35%;CCK-8显示GO纳米载药复合物在50μg·mL~(-1)范围内使用时,无细胞毒性。提示:以GO为载体合成的纳米复合物在生物学中具有广阔的应用前景,值得进一步研究。 相似文献
6.
《饲料工业》2021,(20)
为研究由枯草芽孢杆菌[Bacillus subtilis,(6.7±1.8)×10~7CFU/g]、嗜酸乳杆菌[Lactobacilli acidophilus,(2.1±0.6)×10~8CFU/g]和酿酒酵母[Saccharomyces cerevisiae,(8.4±2.3)×10~6CFU/g]组成的复合益生菌对大菱鲆(Scophthalmus maximus)幼鱼免疫及抗病力的影响,在以鱼粉和豆粕为主要蛋白源的基础饲料中分别添加0%[0 g/kg,对照(Z_0)组]、0.2%(2 g/kg,Z_1组)、0.4%(4 g/kg,Z_2组)、0.6%(6 g/kg,Z_3组)、0.8%(8 g/kg,Z_4组)的复合益生菌粉,制成5组饲料分别投喂体质量为(8.94±0.02)g的大菱鲆60 d。结果显示:大菱鲆血清酸性磷酸酶(ACP)活性,与Z_0组相比,Z_1、Z_2、Z_3组的呈增高趋势,但差异不显著(P0.05),Z_4组与Z_0组的差异也不显著(P0.05),但显著低于Z_1、Z_2、Z_3组(P0.05)。与Z_0组相比,大菱鲆血清的酶活性发生了以下变化:Z_2组的碱性磷酸酶(AKP)、Z3组的溶菌酶(LZM)、Z_1～Z_4组的过氧化氢酶(CAT)、Z_1～Z_3的组的超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)及攻毒后大菱鲆的成活率均显著提高(P0.05)。综上,饲料中添加复合益生菌粉能增强大菱鲆免疫酶活性和抗病力,适宜添加量为0.6%。 相似文献
7.
采用超高效液相色谱-串联质谱法(UPLC-MS/MS),结合PRIME HLB快速通过式前处理技术,优化样品前处理及色谱-质谱条件,建立了同时提取、动态多反应监测(MRM)的测定β-内酰胺类、抗病毒类、大环内酯类、喹诺酮类、四环素类、林可胺类、磺胺类、氯霉素类和氟虫腈及其代谢物共9类38种药物残留的新方法。鸡蛋样品经80%(V/V)乙腈-水溶液(含0.02 mol/L EDTA)提取后,PRIME HLB固相萃取柱净化,基质匹配外标法定量。38种药物在3个不同浓度水平下,平均回收率为71.4%～108.2%;相对标准偏差RSD为0.7%～8.1%;检出限(LOD,S/N=3)和定量限(LOQ,S/N=10)分别为0.01～1.0μg/kg和0.05～3.5μg/kg。本方法简便快速,灵敏可靠,适用于鸡蛋中药物多残留高通量快速检测分析。 相似文献
8.
桔梗的“引经”作用对氟苯尼考药动学的影响 总被引：3，自引：0，他引：3
20只新西兰白兔随机均分成2组(A、B组),A组单剂量1次灌胃内服氟苯尼考30 mg/kg;B组氟苯尼考30mg/kg与桔梗煎液2 g/kg单荆量1次同时灌胃内服给药;采用高效液相色谱法测定血药浓度,血药浓度最低检测限为0.05 mg/L,以3p97药代动力学程序和SPSSl2.0统计分析软件对所得数据进行分析.结果,药-时数据均符合一级吸收一室开放模型(W=1/C2),主要药代动力学参数及拟合方程如下.A组:T1/2ka(0.461±0.066)h,T1/2kc(2.013±0.195)h,Tpeak(1.180±0.123)h,Cmax(7.332±1.000)mg/L,AUC(31.445±3.566)mg·h-1·L-1,V/F(2.995±0.330)L/kg,拟合方程为:C=19.833(e-0.396t-e-2.387t);B组;T1/2ka(0.550±0.112)h,T1/2ke(3.308±0.270)h,Tpeak(1.414±0.183)h,Cmax(4.737±0.360)mg·L-1,AUC(23.128±2.096)mg·h-1·L-1,V/F(1.400±0.127)L/kg,拟合方程为:C-11.021(e-0.342t-e-10860t).结果表明,桔梗与氟苯尼考同时灌胃内服后,桔梗的"引经"对氟苯尼考在家兔体内药物代谢有较大影响. 相似文献
9.
超高效液相色谱-串联质谱检测鸡蛋、鸡肉和猪肉中酰胺醇类药物残留 总被引：1，自引：0，他引：1
《畜牧与兽医》2020,(6)
为了检测畜禽产品中酰胺醇类药物残留是否超标,采用超高效液相色谱-串联质谱法建立了同时检测鸡蛋、鸡肉和猪肉中氯霉素、甲砜霉素、氟苯尼考及其代谢产物氟苯尼考胺等酰胺醇类药物的分析方法。样品经乙腈∶氨水(98∶2,V/V)和乙酸乙酯提取、氮吹、CNWBOND Si柱净化、正己烷去脂后,经ACQUITY UPLC CSH C18 (100 mm×2.1 mm,1.7μm)柱分离,以2 mmol/L乙酸铵水溶液和2 mmol/L乙酸铵乙腈溶液为流动相进行梯度洗脱,流速为0.3 mL/min,多反应监测模式(MRM)检测,分别以氯霉素-d5为氯霉素、甲砜霉素内标,以氟苯尼考-d3为氟苯尼考、氟苯尼考胺内标进行内标法定量。结果显示:4种药物在0.2～100 ng/mL范围内均成良好线性关系,相关系数均在0.99以上,方法检测限为0.1μg/kg,定量限为0.5μg/kg,在0.5～2.0μg/kg添加范围内,回收率范围为70.6%～112.3%,批内相对标准偏差为1.5%～12.7%,批间相对标准偏差为3.2%～14.5%。结果表明该方法灵敏度高,稳定性好,能满足同时检测畜禽产品中多种酰胺醇类药物残留的分析要求。 相似文献
10.
蟹源地衣芽孢杆菌培养条件的优化 总被引：1，自引：0，他引：1
以牛肉膏蛋白胨培养基为初始培养基,探讨了不同培养时间、初始pH值、温度、盐度(NaCl浓度)和转速对中华绒螯蟹源地衣芽孢杆菌ESB3生长的影响。结果表明:菌株ESB3产最大活菌数的培养条件为培养时间20h,初始pH为7.8,温度为30℃左右,盐度为1.5%(m/V),转速为190r/min。采用单因子试验和正交试验优化了种子培养基的组成,结果表明,最佳种子培养基:可溶性淀粉(2%)(m/V)、胰蛋白胨(2%)(m/V)、K2HPO4(0.3%)(m/V),此条件下菌株ESB3活菌数达11.09×108CFU/mL。 相似文献
11.
Benjamin J. Schlegel Victoria J. Nowell Valeria R. Parreira Glenn Soltes John F. Prescott 《Canadian journal of veterinary research》2012,76(4):248-254
This study examined known or possible virulence-associated genes in type A Clostridium perfringens from cases of both bovine clostridial abomasitis (BCA) and jejunal hemorrhage syndrome (JHS) and compared these to isolates from calves that were healthy or had undifferentiated diarrheal illness. A real-time polymerase chain reaction (PCR) assay was used to genotype the 218 C. perfringens isolates. Isolates were sourced from healthy and diarrheic young and mature cattle (n = 191), from calves with confirmed or suspected BCA (n = 22), and from mature cattle with JHS (n = 5). Of 216 isolates (96%), 208 were positive for the cpa gene and 13% (29/218) were positive for atypical cpb2. Three of 8 (37.5%) confirmed BCA isolates, 2 of 13 (15.4%) suspected BCA isolates, and no JHS isolates tested positive for atypical cpb2. As all isolates were negative for cpb, cpb2, cpe, etx, netB, and tpeL, the results of the present study do not support a role for these genes in BCA or JHS. A subset of unique genes identified in 1 bovine clostridial abomasitis isolate (F262), for which a genome sequence is available, was searched for in 8 BCA isolates by PCR. None of the 10 genes was consistently present in all or even in a majority of BCA isolates. Many of these genes were also variably and inconsistently present in type A isolates from calves that did not have BCA. Although a virulence signature to aid in the diagnosis of BCA caused by C. perfringens type A was not identified, further work may discover a gene or group of genes that would constitute such a signature. 相似文献
12.
仔猪痢清口服液的急性毒性实验研究 总被引：2，自引：1，他引：1
为了了解仔猪痢清口服液在使用过程中的安全性,参照《兽药试验技术规范汇编》中有关急性毒性试验的要求,选用小白鼠进行急性毒性试验。在试验中,灌胃给药的最大剂量已达22．50g/kg（生药）,腹腔注射给药的最大剂量已达8．65g/kg（生药）,结果显示没有对小鼠造成任何可见的毒性反应,判定为实际无毒,表明仔猪痢清口服液是一种安全的中草药制剂。 相似文献
13.
Single intravenous and oral dose pharmacokinetics of florfenicol in the channel catfish (Ictalurus punctatus) 总被引：1，自引：0，他引：1
Gaunt PS Langston C Wrzesinski C Gao D Adams P Crouch L Sweeney D Endris R 《Journal of veterinary pharmacology and therapeutics》2012,35(5):503-507
Plasma distribution and elimination of florfenicol in channel catfish were investigated after a single dose (10 mg/kg) of intravenous (i.v.) or oral administration in freshwater at a mean water temperature of 25.4 °C. Florfenicol concentrations in plasma were analyzed by means of liquid chromatography with MS/MS detection. After i.v. florfenicol injection, the terminal half-life (t(1/2)), volume of distribution at steady state (V(ss)), and central volume of distribution (V(c)) were 8.25 h, 0.9 and 0.381 L/kg, respectively. After oral administration of florfenicol, the terminal t(1/2), C(max), T(max), and oral bioavailability (F) were 9.11 h, 7.6 μg/mL, 9.2 h, and 1.09, respectively. There was a lag absorption time of 1.67 h in oral dosing. Results from these studies support that 10 mg florfenicol/kg body weight in channel catfish is an efficacious dosage following oral administration. 相似文献
14.
L D Rowe D E Corrier J C Reagor L P Jones 《American journal of veterinary research》1987,48(6):992-997
The dried, ground aerial portions of the plant Cassia roemeriana were administered to each of seven calves at a dosage of 10 g/kg of body weight/day for 2 to 10.5 days and to each of six goats at a dosage of 10 g/kg/day for 5 days or 5 or 7 g/kg/day for 23 to 25 days. Experimentally induced C roemeriana poisoning in both species resulted in hepatopathic poisoning characterized by a brief survival period (3.9 to 7.9 days), moderate-to-severe hepatocellular damage, and little or no evidence of injury to skeletal muscle or resulted in myopathic poisoning characterized by a longer period of survival, mild-to-severe skeletal myopathy, and mild hepatocellular injury. The minimal dosage that induced hepatopathic poisoning (also the minimal lethal dosage) was 10 g/kg/day for 3 days in calves and for 5 days in goats. The minimal dosage that induced the myopathic syndrome (as determined by the earliest increase in serum creatine kinase activity) was 10 g/kg/day for 6 days for calves and 5 g/kg/day for 10 to 16 days for goats. 相似文献
15.
建立了测定珠蛋白肽对血管紧张素转化酶抑制活性的高效液相色谱分析方法。以马尿酰-组胺酰-亮氨酸为底物,血管紧张素转化酶为催化剂,反应产物马尿酸为检测指标,未加珠蛋白肽的反应为空白对照。最佳色谱条件为:ZorbaxSB-C18(4.6mm×260mm,粒径5μm),柱温25℃,流动相为乙腈/水=25/75[体积比,各含0.05%三氟乙酸(体积分数)],流速1.0mL/min,紫外检测器,检测波长为228nm,进样量10μL。马尿酸浓度为0.005～1mmol/L,线性相关系数为0.9998,最低检测浓度为1μmol/L,回收率在96.66%～101.34%之间,相对标准偏差为1.34%之间。该方法操作简单、分析时间短、准确性和精密度高,利于对珠蛋白肽生产进行质量控制。 相似文献
16.
本研究旨在建立定量检测猪瘟病毒（CSFV）E2重组蛋白的双抗体夹心ELISA方法。用 CSFV WH303单克隆抗体包被96孔酶标板,用CSFV 1B6单克隆抗体连接HRP作为酶标抗体,以杆状病毒表达纯化的CSFV E2蛋白作为标准品蛋白,建立双抗体夹心ELISA定量方法。用SDS-PAGE方法检测标准品蛋白的纯度,BCA蛋白定量法定量标准品蛋白的浓度。稀释标准品至线性区间,绘制标准曲线。用棋盘法确定包被单克隆抗体和酶标抗体及标准品蛋白的最适稀释度。用批间重复和批内重复试验验证该方法的重复性和稳定性。SDS-PAGE结果显示,CSFV E2蛋白的标准品纯度>95%,BCA蛋白定量法测定CSFV E2蛋白的标准品浓度为1.553 mg/mL。包被单克隆抗体的浓度为0.1 μg/mL,酶标抗体的最适稀释度为1∶400。CSFV E2蛋白标准品稀释浓度在2.43~77.65 μg/mL范围内有很好的线性关系,相关系数R2值在0.99以上。批间和批内重复性检测试验变异系数均<15%。本试验成功建立了定量检测CSFV E2重组蛋白的双抗体夹心ELISA检测方法,该方法具有良好的重复性和稳定性,为CSFV E2蛋白的定量提供了有效方法。 相似文献
17.
Abstract AIM: To determine the pharmacokinetics and bioavailability of florfenicol in the plasma of healthy Japanese quail (Coturnix japonica). METHODS: Sixty-five quail were given an I/V and I/M dose of florfenicol at 30 mg/kg bodyweight (BW). A two-period sequential design was used, with a wash-out period of 2 weeks between the different routes of administration. Concentrations of florfenicol in plasma were determined using high-performance liquid chromatography (HPLC). RESULTS: A naíve pooled data analysis approach for the plasma concentration-time profile of florfenicol was found to fit a non-compartmental open model. After I/V administration, the mean residence time (MRT), mean volume of distribution at steady state (Vss), and total body clearance of florfenicol were 12.0 (SD 0.37) h, 8.7 (SD 0.22) L/kg, and 1.3 (SD 0.08) L/h/kg, respectively. After I/M injection, the MRT, mean absorption time (MAT), and bioavailability were 12.3 (SD 0.37) h, 0.2 (SD 0.02) h, and 79.1 (SD 1.79)%, respectively. CONCLUSIONS: The time for the concentration of florfenicol to fall below the probable effective concentration of 1 µg/ml of approximately 10 h is sufficient for the minimum inhibitory concentration needed for many bacterial isolates. Further pharm acodynamic studies in quail are needed to evaluate a suitable dosage regimen. 相似文献
18.
试验旨在研究果香味剂对奶牛乳腺上皮细胞培养液中乳成分含量及培养液风味的影响。试验选用自制的奶牛乳腺上皮细胞,试验设5个处理组,果香味剂的添加剂量分别为0、5、10、15和20μg/ml。结果表明:①当培养基中果香味剂添加量为15μg/ml时,乳腺上皮细胞培养液中总蛋白水平、甘油三酯及乳糖含量均达到最高,其中对脂肪含量影响显著(P<0.05),而对蛋白和乳糖含量影响不显著(P>0.05),之后呈下降趋势,说明果香味剂可能会诱导奶牛乳腺上皮细胞乳蛋白、乳脂和乳糖分泌水平的升高;②运用PT/GC-MS分析乳腺培养液中的风味成分,与对照组相比,各试验组中除具有原有乳腺培养液的风味外,还有乙酸异丁酯、乙酸异戊酯、丙酸异戊酯、丙酸戊酯、丁酸异丁酯、丁酸戊酯、丁酸乙酯、邻苯二甲酸二异丁酯等物质成分,而这些物质成分正好是果香味剂中进入到乳腺培养液中的风味物质,且当添加剂量为15μg/ml时,各物质成分含量为最高(P<0.05),之后呈下降趋势。果香味剂能显著影响奶牛乳腺上皮培养液中乳成分含量和风味。 相似文献
19.
目的：研究不同剂量的当归水煎醇沉液对四氯化碳致小鼠肝损伤的保护作用。方法：取小鼠50只,随机均分为5组：对照组（Ⅰ）、模型组（Ⅱ）、当归高剂量组（Ⅲ）、当归中剂量组（Ⅳ）、当归低剂量组（Ⅴ）。造模前,Ⅲ、Ⅳ、Ⅴ组小鼠分别按20.0、10.0、5.0g/kg皮下注射当归水煎醇沉液,Ⅰ、Ⅱ组注射等量生理盐水,1次/d,连续6d,末次给药后2h,Ⅱ、Ⅲ、Ⅳ、Ⅴ组小鼠腹腔注射0.1%四氯化碳石蜡油溶液（0.1mL/10g）建立肝损伤模型,Ⅰ组注射等量石蜡油。禁食不禁水,24h后处死小鼠。取肝脏左叶相同部位组织,10%的甲醛固定,制作组织切片,H.E染色,光镜下观察肝组织结构与病理变化。结果：当归水煎醇沉液高、中、低剂量组肝细胞损害程度轻于模型组。结论：当归水煎醇沉液对小鼠四氯化碳致肝损伤有明显的保护作用。 相似文献
20.
Hoenig M Alexander S Holson J Ferguson DC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(5):529-532
Intravenous glucose tolerance tests (IVGTTs) are used in cats and other species to assess insulin sensitivity. Several dosages have been reported but the dosage that maximally stimulates insulin secretion in cats has not been determined nor has it been compared in lean and obese animals. IVGTTs were performed in 4 lean and 4 obese spayed female cats with 5 glucose dosages: 0.3 (A), 0.5 (B), 0.8 (C), 1.0 (D). and 1.3 (E) g/kg body weight (BW). Each cat received each dosage in a random design. The glucose disposal rate was significantly different only between lean and obese cats at the highest glucose dosage. The area under the curve for insulin increased significantly among A, B, C, and D in lean and among A, B, and C in obese cats but not between D and E in lean and among C, D, and E in obese cats. Baseline insulin secretion was significantly higher (P = .03) and 1st peak insulin secretion was approximately 50% lower in obese as compared to lean cats (P = .03). Lean but not obese cats reached baseline insulin concentrations at all dosages at 120 minutes. We conclude that the glucose dosage for maximal insulin secretion is 1.0 g/ kg BW in lean and 0.8 g/kg BW in obese cats, supporting routine use of 1 g/kg BW to maximally stimulate insulin secretion regardless of body composition. Obese cats showed an abnormal insulin secretion pattern, indicating a defect in insulin secretion with obesity and insulin resistance. 相似文献